Transport Phenomena Commons^™

A Generalized Design For Affinity Chromatography Columns, Lee-Wei Kao, Nien-Hwa Linda Wang

The Summer Undergraduate Research Fellowship (SURF) Symposium

In affinity chromatography, an adsorbent with a high selectivity for a target solute is used to isolate the target molecule from other impurities. With sufficient selectivity, the target molecule can be isolated in a highly purified and concentrated state. Common applications of affinity chromatography include Protein A chromatography for antibody purification and Immobilized Metal Affinity Chromatography (IMAC) for protein purification. The well-known design method based on constant-pattern mass transfer zone analysis does not apply to small feed batches, which are insufficient to form constant-pattern frontal waves. Other literature design methods rely on simulation or experimental trials that can be time-consuming …

Octanol/Water Partitioning Coefficients Of Pcb Mixtures For Environmental Fate And Transport, Phylicia Cicilio

Honors Theses and Capstones

Polychlorinated biphenyls (PCBs) are a human and environmental toxin introduced to the environment from the 1920’s to the 1970’s from manufacturing items such as transformers and capacitors. PCBs remain in the environment today due to their low water solubility and resistance to chemical reaction. Due to their properties PCBs bioaccumulate in the environment and pose health risks to animals and humans, as they are deemed a probable carcinogen by the EPA. Octanol-water partitioning coefficients are a means of measuring how PCBs will travel in the environment, either partitioning to water or into organic carbon. Octanol-water partitioning coefficients will be determined …

Froth Flotation Performance Enhancement By Feed Cavitation And Magnetic Plastic Particle Addition, Mehmet Saracoglu

Theses and Dissertations--Mining Engineering

Froth flotation is the most commonly used process to recover and upgrade the portion of the coal preparation plant feed that has a particle size smaller than 150 microns. Problems that occur when employing froth flotation in the coal industry include i) coal surfaces that are weakly-to-moderately hydrophobic, and ii) flotation systems that are overloaded and limited by insufficient retention time.

Research was performed to evaluate techniques that could be implemented to improve flotation performance under the aforementioned scenarios. Pre-aeration of flotation feed using a cavitation system was extensively evaluated in laboratory and full-scale test programs. The benefits of adding …

The Critical Role Of Mechanism-Based Models For Understanding And Predicting Liposomal Drug Loading, Binding And Release Kinetics, Sweta Modi

Theses and Dissertations--Pharmacy

Liposomal delivery systems hold considerable promise for improvement of cancer therapy provided that critical formulation design criteria can be met. The main objective of the current project was to enable quality by design in the formulation of liposomal delivery systems by developing comprehensive, mechanism-based mathematical models of drug loading, binding and release kinetics that take into account not only the therapeutic requirement but the physicochemical properties of the drug, the bilayer membrane, and the intraliposomal microenvironment.

Membrane binding of the drug affects both drug loading and release from liposomes. The influence of bilayer composition and phase structure on the partitioning …