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Full-Text Articles in Process Control and Systems
Microcystin-Lr Removal From Water Via Enzymatic Linearization And Ultrafiltration, Abelline Fionah, Cannon Hackett, Hazim Aljewari, Laura Brady, Faisal Alqhtani, Isabel C. Escobar, Audie K. Thompson
Microcystin-Lr Removal From Water Via Enzymatic Linearization And Ultrafiltration, Abelline Fionah, Cannon Hackett, Hazim Aljewari, Laura Brady, Faisal Alqhtani, Isabel C. Escobar, Audie K. Thompson
Chemical Engineering Faculty Publications and Presentations
Microcystin-LR (MC-LR) is a toxin produced by cyanobacteria that can bloom in freshwater supplies. This study describes a new strategy for remediation of MC-LR that combines linearization of the toxin using microcystinase A, MlrA, enzyme with rejection of linearized byproducts using membrane filtration. The MlrA enzyme was expressed in Escherichia coli (E. coli) and purified via a His-tag with 95% purity. Additionally, composite membranes made of 95% polysulfone and 5% sulfonated polyether ether ketone (SPEEK) were fabricated and used to filter a solution containing cyclic and linearized MC-LR. Tests were also performed to measure the adsorption and desorption of MC-LR …
Peptoid Microsphere Coatings To Improve Performance In Sandwich Elisa Microarrays, Jesse L. Roberts, German R. Perez Bakovic, Shannon L. Servoss
Peptoid Microsphere Coatings To Improve Performance In Sandwich Elisa Microarrays, Jesse L. Roberts, German R. Perez Bakovic, Shannon L. Servoss
Chemical Engineering Faculty Publications and Presentations
Enzyme-linked immunosorbent assay (ELISA) microarray performance is limited by low assay sensitivity and dynamic range. Increasing the surface area for reagent binding can help to improve performance, but standard techniques such as roughening the surface or adding a polymer coating lead to increased non-specific fluorescence and do not have reproducibly improved performance. Another approach to increase surface area is adding a microsphere coating on the surface. Poly-N-substituted glycine (peptoid) microspheres are ideal for this application due to low immunogenicity, protease-resistance, and biocompatibility. Peptoids are polymers with a backbone similar to peptides, but with the side chains appended to nitrogen rather …