Biochemical and Biomolecular Engineering Commons^™

Development Of A Method Of Oriented Immobilization Of Lipases On A Solid Carrier, Ziyavitdinov Jamolitdin Fazlitdinovich, Inogamov Utkir Kudratillayevich, Berdiev Nodir Sharifovich, Ishimov Uchkun Jomuradovich

CHEMISTRY AND CHEMICAL ENGINEERING

The methods of oriented immobilization of lipases on a solid carrier - Silochrome C-80 have been developed. The resulting sorbents were tested in the process of obtaining biodiesel from cotton oil. It was shown that immobilized lipase on Silochrome C-80 with NH2 group has the highest transetrification ability and stability, while in sorbents with the enzyme immobilized with -COOH and -SH groups this value was 87 and 53%, respectively. Biocatalysis using an affinity sorbent of immobilized lipase with the –NH2 group has a high operational stability, which, after 240 hours of operation, retains more than 50%, and after 480 hours, …

Development Of A Method Of Oriented Immobilization Of Lipases On A Solid Carrier, Ziyavitdinov Jamolitdin Fazlitdinovich, Inogamov Utkir Kudratillayevich, Berdiev Nodir Sharifovich, Ishimov Uchkun Jomuradovich

CHEMISTRY AND CHEMICAL ENGINEERING

The methods of oriented immobilization of lipases on a solid carrier - Silochrome C-80 have been developed. The resulting sorbents were tested in the process of obtaining biodiesel from cotton oil. It was shown that immobilized lipase on Silochrome C-80 with NH2 group has the highest transetrification ability and stability, while in sorbents with the enzyme immobilized with -COOH and -SH groups this value was 87 and 53%, respectively. Biocatalysis using an affinity sorbent of immobilized lipase with the –NH2 group has a high operational stability, which, after 240 hours of operation, retains more than 50%, and after 480 hours, …

Development Of A Method Of Oriented Immobilization Of Lipases On A Solid Carrier, Ziyavitdinov Jamolitdin Fazlitdinovich, Inogamov Utkir Kudratillayevich, Berdiev Nodir Sharifovich, Ishimov Uchkun Jomuradovich

CHEMISTRY AND CHEMICAL ENGINEERING

The methods of oriented immobilization of lipases on a solid carrier - Silochrome C-80 have been developed. The resulting sorbents were tested in the process of obtaining biodiesel from cotton oil. It was shown that immobilized lipase on Silochrome C-80 with NH2 group has the highest transetrification ability and stability, while in sorbents with the enzyme immobilized with -COOH and -SH groups this value was 87 and 53%, respectively. Biocatalysis using an affinity sorbent of immobilized lipase with the –NH2 group has a high operational stability, which, after 240 hours of operation, retains more than 50%, and after 480 hours, …

Development And Validation Of A Kinetic Model For Enzymatic Hydrolysis Using Candida Rugosa Lipase, Ammar Jamie, Ali Alshami, Zuhair O. Maliabari, Muataz A. Ateih

Chemical Engineering Faculty Publications

Biochemical processing involving enzymatic catalysis of hydrolysis reactions of oils and fats must overcome significant technological barriers before the full benefits of the technology can be realized. Owing to their selectivity and mild reaction conditions, lipases are becoming increasingly important as biocatalysts provided that their kinetics and optimum reaction conditions are well-understood. In this study we report on the development and validation of a kinetic model for the degradation of oils using Candida rugosa lipase, from which a better understanding of the influence of different reaction conditions on hydrolysis kinetics is elucidated. Variations of reaction temperature, mixing speed, enzyme loading …

Development And Utilization Of A Pair Of Sol-Gel Entrapped Lipases For Biodiesel Production From High Free Fatty Acid Oils, Cory M. Schwartz

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Biodiesel, which consists of fatty acid alkyl esters, is one of the most widely adopted and successful renewable fuels. Traditional physiochemical biodiesel production methods require high cost refined feedstocks, and so alternative methods of catalysis and feedstocks have been explored. This research investigated the use of polysiloxane entrapped lipases to catalyze the production of biodiesel from low cost feedstocks.

In this work, lipase from Burkholderia cepacia (lipase PS) and lipase B from Candida antarctica (CalB) were separately entrapped using sol-gel chemistry. Optimal reaction conditions for the esterification of free fatty acids by immobilized CalB with methanol were determined. Immobilized CalB …

Bioreactor System Designs For Lipase-Catalyzed Synthesis Of Saccharide- Fatty Acid Esters In Solvent-Free Media, Ran Ye

Doctoral Dissertations

As nontoxic biobased surfactants derived from plant oils and cellulose or starch, saccharide-fatty acid esters are widely used in cosmetics, food, and pharmaceutical industries due to their biocompatibility, biodegradability as well as antimicrobial activity. Generally, saccharide-fatty acid esters are synthesized chemically under high pressure, temperature and the presence of alkaline or acid catalysts leading to low-quality products (chemo-degradation of double bonds and oxygenated moieties) and large amounts of byproducts. In contrast, biocatalytic synthesis enhances sustainability: near-ambient pressure and temperature, the absence of toxic, acids and bases catalysts, and improved selectivity of products. For lipase-catalyzed synthesis under nearly anhydrous conditions, the …