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Articles 121 - 121 of 121
Full-Text Articles in Biochemical and Biomolecular Engineering
Optimizing Diabetes Diagnostics: Electrophoretic Separation And Detection Of Hemoglobin A1c With Boronate, Rixlie Fozilova
Optimizing Diabetes Diagnostics: Electrophoretic Separation And Detection Of Hemoglobin A1c With Boronate, Rixlie Fozilova
Dissertations, Master's Theses and Master's Reports
Diabetes affects 537 million people worldwide, while 136 million Americans have diabetes or prediabetes; roughly 22.6% of individuals are undiagnosed. Diabetes is the inability to regulate blood sugar levels. Hemoglobin A1c (HbA1c) is glycosylated hemoglobin and indicates average blood sugar levels over the past three months.
We adapted the selective boronic acid binding to the HbA1c sugar moieties from boronate affinity chromatography and translated it into a portable microchip paper-based electrophoresis platform. We explored a range of boronate concentrations at a steady voltage and sample volume to determine the optimal HbA1c binding and subsequent electrophoretic separation. A pH above 11 …