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Biochemical and Biomolecular Engineering Commons™

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2007

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Articles 1 - 22 of 22

Full-Text Articles in Biochemical and Biomolecular Engineering

Effect Of Sparger Design On Hydrodynamics Of A Gas Recirculation Anaerobic Bioreactor, Rajneesh Varma, Muthanna H. Al-Dahhan Dec 2007

Effect Of Sparger Design On Hydrodynamics Of A Gas Recirculation Anaerobic Bioreactor, Rajneesh Varma, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

The Effects of Sparger Design and Gas Flow Rate On, Gas Holdup Distribution and Liquid (Slurry) Recirculation Velocity Have Been Studied in a Surrogate Anaerobic Bioreactor Used for Treating Bovine Waste with a Conical Bottom Mixed by Gas Recirculation. a Single Orifice Sparger (SOS) and a Multi-Orifice Ring Sparger (MORS) with the Same Orifice Open Area and Gas Flow Rates (Hence the Same Process Power Input) Are Compared in This Study. the Advanced Non-Invasive Techniques of Computer Automated Tomography (CT) and Computer Automated Radioactive Particle Tracking (CARPT) Were Employed to Determine Gas Holdup, Liquid Recirculation Velocity, and the Poorly Mixed …

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Numerical Simulation Of Gas-Solid Dynamics In A Circulating Fluidized-Bed Riser With Geldart Group B Particles, S. Vaishali, Shantanu Roy, Satish Bhusarapu, M. (Muthanna) H. Al-Dahhan, M. P. Dudukovic Dec 2007

Numerical Simulation Of Gas-Solid Dynamics In A Circulating Fluidized-Bed Riser With Geldart Group B Particles, S. Vaishali, Shantanu Roy, Satish Bhusarapu, M. (Muthanna) H. Al-Dahhan, M. P. Dudukovic

Chemical and Biochemical Engineering Faculty Research & Creative Works

Circulating Fluidized-Bed (CFB) Risers with Geldart Group B Particles Have Found Significant Application in Combustion Reactions. the Present Work Attempts to Study the Solids Flow Dynamics in a CFB Riser that is Operated with Group B Particles, using Computational Fluid Dynamics (CFD) Techniques. the Key Feature in the Present Study is that the Various Closure Schemes in the CFD Model Have Been Evaluated Against Data from Non-Invasive Experimental Techniques: Computer Automated Radioactive Particle Tracking (CARPT) for Solids Velocity Field and Computed Tomography (CT) for Solids Holdup. Since Solids Flow in a Riser is Multiscale in Character, in Addition to the …

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Moment Analysis Of Near-Equilibrium Binding Interactions During Electrophoresis, Yousef Daneshbod, James D. Sterling, Ali Nadim Nov 2007

Moment Analysis Of Near-Equilibrium Binding Interactions During Electrophoresis, Yousef Daneshbod, James D. Sterling, Ali Nadim

Business and Information Technology Faculty Research & Creative Works

The electrophoretic transport of three chemically reacting species, two of which can bind reversibly to form the third, is analyzed mathematically. The species are assumed to move horizontally through a long channel with different electrophoretic mobilities and diffusion coefficients. By considering small perturbations of the system about equilibrium or when one of the two binding species is much more abundant than the other, the governing advection-reaction- diffusion equations can be linearized and studied via the method of moments. The result is a set of coupled ordinary differential equations for the moments that can be solved analytically. Analysis of the long-time …

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Phase Transformations Of Calcium Phosphates Formed In Wet Field Environments, O. M. Clarkin, Mark R. Towler, G. M. Insley, M. E. Murphy Oct 2007

Phase Transformations Of Calcium Phosphates Formed In Wet Field Environments, O. M. Clarkin, Mark R. Towler, G. M. Insley, M. E. Murphy

Chemical and Biochemical Engineering Faculty Research & Creative Works

The crystal phase and morphology of calcium phosphate salts precipitated in a wet field environment at temperatures between 30 and 70 °C and pHs between 3 and 8 were examined. Dicalcium Phosphate Dihydrate (DCPD) was the most prevalent phase precipitated. Using accelerated ageing study techniques, precipitates studied were aged, under dry conditions at 50 °C for 8 and 16 days, before being re-examined using XRD, FTIR and SEM techniques. DCPD was found to be most stable when precipitated at 40 °C and 5 pH. Considerably more phase transformation to Octacalcium Phosphate (OCP), Amorphous Calcium Phosphate (ACP) and Hydroxyapatite (HA) was …

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Prediction Of The Kolmogorov Entropy Derived From Computed Tomography Data In A Bubble Column Operated Under The Transition Regime And Ambient Pressure, Stoyan Nedeltchev, Ashfaq Shaikh, Muthanna H. Al-Dahhan Oct 2007

Prediction Of The Kolmogorov Entropy Derived From Computed Tomography Data In A Bubble Column Operated Under The Transition Regime And Ambient Pressure, Stoyan Nedeltchev, Ashfaq Shaikh, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

The Kolmogorov Entropy (KE) Algorithm Was Successfully Applied to Single Source Γ-Ray Computed Tomography (CT) Data Measured by Three Scintillation Detectors in a 0.162 M-ID Bubble Column Equipped with a Perforated Plate Distributor (163 Holes X ∅ 1.32. 10-3 M). the Aerated Liquid Height Was Set at 1.8 M. Dried Air Was Used as a Gas Phase, While Therminol LT (PL = 886 Kg M-3, ΜL = 0.88.10-3 Pa S, Σ = 17.10-3 N M-1) Was Used as a Liquid Phase. at Ambient Pressure, the Superficial Gas Velocity, ΜG, Was Increased …

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Mesophilic Digestion Kinetics Of Manure Slurry, Khursheed Karim, K. Thomas Klasson, Sadie R. Drescher, Whitney Ridenour, Abhijeet P. Borole, Muthanna H. Al-Dahhan Sep 2007

Mesophilic Digestion Kinetics Of Manure Slurry, Khursheed Karim, K. Thomas Klasson, Sadie R. Drescher, Whitney Ridenour, Abhijeet P. Borole, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

Anaerobic Digestion Kinetics Study of Cow Manure Was Performed at 35°C in Bench-Scale Gas-Lift Digesters (3.78 L Working Volume) at Eight Different Volatile Solids (VS) Loading Rates in the Range of 1.11-5.87 G L-1 Day-1. the Digesters Produced Methane at the Rates of 0.44-1.18 L L-1 Day-1, and the Methane Content of the Biogas Was Found to Increase with Longer Hydraulic Retention Time (HRT). based on the Experimental Observations, the Ultimate Methane Yield and the Specific Methane Productivity Were Estimated to Be 0.42 L CH4 (G vs. Loaded)-1 and 0.45 L CH …

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Influence Of Two Changes In The Composition Of An Acrylic Bone Cement On Its Handling, Thermal, Physical, And Mechanical Properties, G. Lewis, J. Xu, S. Madigan, Mark R. Towler Aug 2007

Influence Of Two Changes In The Composition Of An Acrylic Bone Cement On Its Handling, Thermal, Physical, And Mechanical Properties, G. Lewis, J. Xu, S. Madigan, Mark R. Towler

Chemical and Biochemical Engineering Faculty Research & Creative Works

This study is a contribution to the growing body of work on the influence of changes in the composition of an acrylic bone cement on various properties of the curing and cured material. The focus is on one commercially-available acrylic bone cement brand, Surgical Simplex®P, and three variants of it and a series of properties, namely, setting time, maximum exotherm temperature, activation energy and frequency factor for the polymerization reaction, diffusion coefficient for the uptake of phosphate buffered saline, at 37°C, ultimate compressive strength (UCS), plane-strain fracture toughness, fatigue life (under fully-reversed tension-compression stress), hardness (H) and elastic modulus (both …

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A Chemical Engineering Laboratory Over Distributed Control And Measurement Systems, Jing Guo, David Kettler, Muthanna H. Al-Dahhan Aug 2007

A Chemical Engineering Laboratory Over Distributed Control And Measurement Systems, Jing Guo, David Kettler, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

Remote Control of Lab Apparatus Via the Internet Allows Flexible Scheduling of Experiments and Exercises. This Paper Presents a Novel Application of Computers and Internet Technology in Chemical Engineering Education that Highlights Interactive Learning for an Undergraduate Course in Online Measurement and Control. the Proposed Client-Server Architecture Can Be Adapted to Drive Different Kinds of Devices and Can Be Easily Expanded. the Developed Online Laboratory Has Been Tested by Many Students using Different Connections and Operating Conditions. This Internet-Based Solution Can Improve Undergraduate Engineering Education in a Conventional Unit Operation Laboratory. as an Example, the Application of Remote Experiments to …

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Anwendung Von Interferenz- Und Ir-Mikroskopie Zur Charakterisierung Und Untersuchung Des Stofftransportes In Nanoporösen Materialien, Lars Heinke, Christian Chmelik, Pavel Kortunov, Sergey Vasenkov, Douglas M. Ruthven, Dhananjai B. Shah, Jörg Kärger Aug 2007

Anwendung Von Interferenz- Und Ir-Mikroskopie Zur Charakterisierung Und Untersuchung Des Stofftransportes In Nanoporösen Materialien, Lars Heinke, Christian Chmelik, Pavel Kortunov, Sergey Vasenkov, Douglas M. Ruthven, Dhananjai B. Shah, Jörg Kärger

Chemical & Biomedical Engineering Faculty Publications

Die Anwendung der Interferenz-Mikroskopie bedeutet einen entscheidenden Durchbruch in der Untersuchung des Stofftransports von Gastmolekülen in nanoporösen Wirtsmaterialien. Diese Technik ermöglicht die Beobachtung der zeitaufgelösten intrakristallinen Konzentrationsprofile, die durch eine Änderung des umgebenden Gasdruckes entstehen. Dieser Ansatz wurde zur Untersuchung von Adsorptions- und Desorptionsprozessen in verschiedenen Zeolithsystemen verwendet. Die Vorteile dieser Technik, vor allem wenn sie mit Infrarotabsorptionsmessungen kombiniert wird, werden an Hand der experimentellen Resultate des Methanol-in-Ferrierit-Systems illustriert.

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Influence Of Acid Washing On The Surface Morphology Of Ionomer Glasses And Handling Properties Of Glass Ionomer Cements, C. M. Crowley, J. Doyle, Mark R. Towler, N. Rushe, S. Hampshire Aug 2007

Influence Of Acid Washing On The Surface Morphology Of Ionomer Glasses And Handling Properties Of Glass Ionomer Cements, C. M. Crowley, J. Doyle, Mark R. Towler, N. Rushe, S. Hampshire

Chemical and Biochemical Engineering Faculty Research & Creative Works

Acid washing is known to influence the handling properties of ionomer glasses used in glass ionomer cements due to the production of an ion depleted zone on the surface of the glass particles. The influence of acid washing on the particle size distribution and surface area of four glasses was examined by scanning electron microscopy (SEM), particle size analysis (PSA) and accelerated surface area porosimetry (ASAP) and the working and setting times of cements, produced from the glasses, correlated to changes in surface morphology. A linear relationship was found between the specific surface area of acid-washed SiO2-Al2 …

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Software Engineering: A Fundamental Approach To Automate And Interface Medical Electronic Systems With Computers In Healthcare Systems, Hashim Twaakyondo Jun 2007

Software Engineering: A Fundamental Approach To Automate And Interface Medical Electronic Systems With Computers In Healthcare Systems, Hashim Twaakyondo

Tanzania Journal of Engineering and Technology (TJET)

The cost for application software and propriatry standards (i.e. data structure and formats) required for storing clinical datasets from electronic device cause the use of time-consuming paper-based documentation and/or the transfer of electronic lab records manually to the PC database systems in Mother-Offspring Malaria Study (MOMS) Laboratory Unit located in Morogoro Regional Hospital, Tanzania. We have explored the ability to extract laboratory results from Haematology analyzer machine for easy management, access and storage. The methodology and tools used during the study includes; site survey and hands-on practice for requirement engineering, visual basic 6.0 for interface design and Microsoft Access for …

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Oaming Characteristics Of The Food-Industry Biological Wastewater Treatment Plant, Samwel V. Manyele Jun 2007

Oaming Characteristics Of The Food-Industry Biological Wastewater Treatment Plant, Samwel V. Manyele

Tanzania Journal of Engineering and Technology (TJET)

Intensive foaming observed during aeration of the aerobic food industry wastewater treatment plant is presented. This study was conducted to establish the foaming characteristics using a pilot three-phase fluidized bed bioreactor (TPFBB). A fluidization column (0.2 m i.d. and 6 m high) was used. The TPFBB was loaded with variable masses of novel biomass support particles (BSP). Wastewater samples collected from a plant processing both vegetable oil (VOPWW) and soap (SPWW) were immediately introduced in the TPFBB for aeration. The characteristics studied include: variation of foam volume with time, foam multiplicity, foaming and foam collapse rates. The SPWW produced excessive …

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Using A Fiber-Optic Probe For The Measurement Of Volumetric Expansion Of Liquids, Sean G. Mueller, Jay R. Werber, Muthanna H. Al-Dahhan, Milorad P. Dudukovic Jun 2007

Using A Fiber-Optic Probe For The Measurement Of Volumetric Expansion Of Liquids, Sean G. Mueller, Jay R. Werber, Muthanna H. Al-Dahhan, Milorad P. Dudukovic

Chemical and Biochemical Engineering Faculty Research & Creative Works

A Fiber-Optic Probe is Developed for the Fast, In-Situ Measurement of Volumetric Expansion of Multiphase and Multicomponent Systems. an Experiment with the Binary Mixtures of CO2-Toluene and CO2-Ethanol Was Conducted to Demonstrate the Usefulness of the Fiber-Optic Probe in Accurately Tracking the Isothermal Volumetric Expansion as a Function of Pressure. in the 1-L Autoclave that Has Been Used, the Probe Was Shown to Detect the Liquid Level Height within a Precision of 0.35% of the Total Height of the Vessel. the Results for the Volumetric Expansion of Toluene and Ethanol with CO2 Correlate Well with …

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Measuring Gas-Liquid Distribution In A Pilot Scale Monolith Reactor Via An Industrial Tomography Scanner (Its), M. (Muthanna) H. Al-Dahhan, A. Kemoun, A. R. Cartolano, S. Roy, R. Dobson, J. Williams Jun 2007

Measuring Gas-Liquid Distribution In A Pilot Scale Monolith Reactor Via An Industrial Tomography Scanner (Its), M. (Muthanna) H. Al-Dahhan, A. Kemoun, A. R. Cartolano, S. Roy, R. Dobson, J. Williams

Chemical and Biochemical Engineering Faculty Research & Creative Works

An Industrial Tomography Scanner (ITS) Was Designed and Developed to Study and Quantify the Phase Distribution in a Two-Phase Flow Pilot Scale Monolith Reactor that Was 24 In. (0.60 M) in Diameter and 192 In. (4.9 M) in Height. the Monolith Reactor Was Operated Co-Current Up-Flow in the Taylor Flow Regime with Water as the Liquid Phase and Air as the Gas Phase. the Cross-Sectional Holdup Distributions Were Measured at Three Axial Elevations. the Operating Conditions Were Selected to Bracket Commercial Operating Conditions for Fixed Bed Monolithic Reactor Systems. the Results Show that its Can Capture the Flow Features in …

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Preliminary Work On The Development Of A Novel Detection Method For Osteoporosis, P. Moran, Mark R. Towler, S. Chowdhury, J. Saunders, M. J. German, N. S. Lawson, H. M. Pollock, I. Pillay, D. Lyons Jun 2007

Preliminary Work On The Development Of A Novel Detection Method For Osteoporosis, P. Moran, Mark R. Towler, S. Chowdhury, J. Saunders, M. J. German, N. S. Lawson, H. M. Pollock, I. Pillay, D. Lyons

Chemical and Biochemical Engineering Faculty Research & Creative Works

Osteoporosis affects both the organic and mineral phases of bone resulting in a decrease in resistance to fracture. Dual x-ray absorptiometry (DEXA) scans are used for diagnosing osteoporosis, which is conventionally characterized by a decrease in mineral density. Unfortunately, some patients who suffer osteoporotic fractures have normal bone density, because both the organic and the mineral phase are affected. However, there are currently no methods of evaluating the health of the organic phase. Patients undergoing treatment for osteoporosis have reported hardening of their fingernails. As the properties of nail and bone may be linked in a comparable, measurable way, this …

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Pt-Ru Nanoparticles Supported On Carbon Nanotubes As Methanol Fuel Cell Catalysts, Liang Li, Yangchuan Xing May 2007

Pt-Ru Nanoparticles Supported On Carbon Nanotubes As Methanol Fuel Cell Catalysts, Liang Li, Yangchuan Xing

Chemical and Biochemical Engineering Faculty Research & Creative Works

Bimetallic Pt - Ru alloy catalysts have been demonstrated to be more active than pure Pt catalysts in the electrooxidation of methanol. We report here a study on Pt - Ru nanoparticle catalysts supported on sonochemically functionalized carbon nanotubes. the catalysts were prepared by directly reducing the corresponding salts, K2PtCl4 and K 2RuCl5, in an ethylene glycol aqueous solution containing dispersed carbon nanotubes. Three catalysts of different Pt to Ru atomic ratios, namely, Pt53Ru47, Pt69Ru31, and Pt77Ru23, were prepared for investigation of the compositional effects, It was shown that highly dispersed bimetallic Pt - Ru alloy nanoparticles with no agglomeration …

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Raman Spectroscopy Of The Human Nail: A Potential Tool For Evaluating Bone Health?, Mark R. Towler, A. Wren, N. Rushe, J. Saunders, N. M. Cummins, P. M. Jakeman May 2007

Raman Spectroscopy Of The Human Nail: A Potential Tool For Evaluating Bone Health?, Mark R. Towler, A. Wren, N. Rushe, J. Saunders, N. M. Cummins, P. M. Jakeman

Chemical and Biochemical Engineering Faculty Research & Creative Works

Dual X-ray absorptiometry (DEXA) is the current gold standard for the diagnosis of osteoporosis. However, patients can suffer osteoporotic fractures despite normal bone mineral density, partly because of unmeasured influences of both the protein and mineral phases of bone that are affected in osteoporosis. There is currently no clinically applicable method of evaluating the health of the protein phase. The proteins in human nail (keratin) and bone (collagen) require sulphation and disulphide bond (S-S) formation for structural integrity and disorders of either sulphur metabolism or cystathione beta-synthase can lead to structural abnormalities in these tissues. Raman protein spectra provide a …

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Identification And Characterization Of Calcium And Manganese Transporting Atpase (Pmr1) Gene Of Pichia Pastoris, Michael P. Dux, Mehmet Inan Feb 2007

Identification And Characterization Of Calcium And Manganese Transporting Atpase (Pmr1) Gene Of Pichia Pastoris, Michael P. Dux, Mehmet Inan

Papers in Biotechnology

A gene homologous to Saccharomyces cerevisiae PMR1 has been cloned in the methylotrophic yeast Pichia pastoris.The entire P. pastoris PMR1 gene (PpPMR1 ) codes a protein of 924 amino acids. Sequence analysis of the PpPMR1 cDNA and the genomic DNA revealed that there is no intron in the coding region. The putative gene product contains all of the conserved regions observed in P-type ATPases and exhibits 66.2%, 60.3% and 50.6% identity to Pichia angusta (Hansenula polymorpha), Saccharomyces cerevisiae PMR1 and human ATP2C1 gene products, respectively. A pmr1 null mutant strain of P. pastoris exhibited growth defects in media with the …

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Mechanical Characterization Of Spherical Hydrogels: Contact Diameter Measurement, Joshua Bunch Jan 2007

Mechanical Characterization Of Spherical Hydrogels: Contact Diameter Measurement, Joshua Bunch

Opportunities for Undergraduate Research Experience Program (OURE)

In an effort to develop new ophthalmic treatments revolving around the ocular lens, the mechanical characteristics of natural ocular lenses and synthetic models must be determined and compared. One mechanical characteristic that is necessary when making comparisons is the variation of contact circle diameter or contact area with varying strains. A method was used in this experiment to measure the varying contact circle diameter of spherical hydrogels at strains up to 15%. This method could be used to estimate the varying contact diameter of other similar materials such as natural ocular lenses if the precision of the method is improved. …

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Cell Bank Characterization And Fermentation Optimization For Production Of Recombinant Heavy Chain C-Terminal Fragment Of Botulinum Neurotoxin Serotype E (Rbonte(HC): Antigen E) By Pichia Pastoris, Jayanta Sinha, Mehmet Inan, Sarah Fanders, Shinichi Taoka, Mark Gouthro, Todd Swanson, Rick Barent, Ardis Barthuli, Bonnie M. Loveless, Leonard A. Smith, Theresa Smith, Ian Henderson, John Ross, Michael M. Meagher Jan 2007

Cell Bank Characterization And Fermentation Optimization For Production Of Recombinant Heavy Chain C-Terminal Fragment Of Botulinum Neurotoxin Serotype E (Rbonte(HC): Antigen E) By Pichia Pastoris, Jayanta Sinha, Mehmet Inan, Sarah Fanders, Shinichi Taoka, Mark Gouthro, Todd Swanson, Rick Barent, Ardis Barthuli, Bonnie M. Loveless, Leonard A. Smith, Theresa Smith, Ian Henderson, John Ross, Michael M. Meagher

Papers in Biochemical Engineering

A process was developed for production of a candidate vaccine antigen, recombinant C-terminal heavy chain fragment of the botulinum neurotoxin serotype E, rBoNTE(Hc)in Pichia pastoris. P. pastoris strain GS115 was transformed with the rBoNTE(Hc) gene inserted into pHILD4 Escherichia coli—P. pastoris shuttle plasmid. The clone was characterized for genetic stability, copy number, and BoNTE(Hc) sequence. Expression of rBoNTE(Hc) from the Mut+ HIS4 clone was confirmed in the shake-flask, prior to developing a fed-batch fermentation process at 5 and 19 L scale. The fermentation process consists of a glycerol growth phase in …

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Heat Transfer Coefficients In A High-Pressure Bubble Column, Chengtian Wu, Muthanna H. Al-Dahhan, Anand Prakash Jan 2007

Heat Transfer Coefficients In A High-Pressure Bubble Column, Chengtian Wu, Muthanna H. Al-Dahhan, Anand Prakash

Chemical and Biochemical Engineering Faculty Research & Creative Works

Time-Averaged Local Heat Transfer Coefficients Were Studied in a 0.16 M Inner Diameter High-Pressure Air-Water Bubble Column. the Effects of the Superficial Gas Velocity (Up to 0.30 M/s), Pressure (Up to 10 Bar), Probe Position, and Probe Orientation Were Investigated. the Heat Transfer Coefficients Increased with Superficial Gas Velocity, and the Values in the Center of the Column Were 9-16% Greater Than Those Near the Wall Region under Atmospheric Pressure (1 Bar). with the Increase in Pressure, the Heat Transfer Coefficients Decreased Due to Combined Effects of Bubble Size Decreasing, Gas Holdup Increasing, and Bubble Number Increasing. the Heat Transfer …

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Gas-Liquid Mass Transfer In A High Pressure Bubble Column Reactor With Different Sparger Designs, Lu Han, Muthanna H. Al-Dahhan Jan 2007

Gas-Liquid Mass Transfer In A High Pressure Bubble Column Reactor With Different Sparger Designs, Lu Han, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

The Gas-Liquid Mass Transfer in a 0.162 M High-Pressure Stainless-Steel Bubble Column Was Investigated using Three Different Gas Sparger Designs. an Oxygen-Enriched-Air Dynamic Method and an Optical Oxygen Probe Technique Were Implemented to Measure K1 a Values in the Bubble Column Reactor. using the Interfacial Area (A) Values Measured by a Four-Point Optical Probe Technique at Similar Conditions (Xue, 2004), the K1 Values Were Estimated. Axial Dispersion Model (ADM) and Continuous Stirred Tank Reactor (CSTR) Model Were Used to Calculate K1 a as a Fitted Parameter with the Measured Data. the ADM Gave Better Fits to the Experimental Data Than …

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