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Biochemical and Biomolecular Engineering Commons™

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2003

Articles 1 - 17 of 17

Full-Text Articles in Biochemical and Biomolecular Engineering

Centrifugal Extraction Of Avocado Oil From The Pulp, J. W. Ntalikwa Dec 2003

Centrifugal Extraction Of Avocado Oil From The Pulp, J. W. Ntalikwa

Tanzania Journal of Engineering and Technology (TJET)

Absract

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Kinetics Of Wet Air Oxidation Of Phenol Over A Novel Catalyst, Jing Guo, Muthanna H. Al-Dahhan Oct 2003

Kinetics Of Wet Air Oxidation Of Phenol Over A Novel Catalyst, Jing Guo, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

The Wet Air Oxidation Kinetics of an Aqueous Solution of Phenol Was Studied over Extrudates of an Al-Fe Pillared Clay Catalyst in the Temperature Range of 90-150°C and Air Pressure Range of 0.8-2.5 MPa. the Variables Studied Included Reaction Temperature, Air Pressure, Solution PH, Initial Phenol Concentration, and Catalyst Loading. the Obtained Findings Are Compared with Those Obtained using Hydrogen Peroxide Oxidation. a Group of Kinetic Models Considering Both the Power-Law and Langmuir-Hinshelwood Approaches Was Evaluated to Describe the Catalytic Kinetics of the Phenol Disappearance. the Nonlinear Dependence of the Phenol Conversion Rate on the Catalyst Concentration Was Taken into …

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Computation Of Effectiveness Factors For Partially Wetted Catalyst Pellets Using The Method Of Fundamental Solution, E. Palmisano, P. A. Ramachandran, K. Balakrishnan, M. (Muthanna) H. Al-Dahhan Oct 2003

Computation Of Effectiveness Factors For Partially Wetted Catalyst Pellets Using The Method Of Fundamental Solution, E. Palmisano, P. A. Ramachandran, K. Balakrishnan, M. (Muthanna) H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

Trickle Bed Reactors Are Widely Used in Many Process Industries. the Catalyst Particles Are Often Incompletely Wetted Especially in the Trickling Flow Regime and Hence to Design These Reactors, the Effectiveness Factor of Partially Wetted Catalyst Needs to Be Calculated Accurately. Numerical Solutions by Traditional Methods Are Time Consuming and Not Very Accurate, Especially for Some Commonly Used Complex Catalyst Shapes Such as Trilobes, Quadrilobes Etc. the Paper Presents a Novel Numerical Solution for These Problems based on the Method of Fundamental Solutions. the Advantage of the Method is that It Involves Only Boundary Collocation and Can Be Applied to …

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Preliminary Work On The Antibacterial Effect Of Strontium In Glass Ionomer Cements, A. Guida, Mark R. Towler, J. G. Wall, R. G. Hill, S. Eramo Oct 2003

Preliminary Work On The Antibacterial Effect Of Strontium In Glass Ionomer Cements, A. Guida, Mark R. Towler, J. G. Wall, R. G. Hill, S. Eramo

Chemical and Biochemical Engineering Faculty Research & Creative Works

The antibacterial effect of strontium in glass ionomer cements (GIC) was discussed. The glass transition temperatures were found to decrease with the increasing fluorine content consistent with the fluorine replacing bridging oxygens. However no significant correlation was found between the fluoride release and the antibacterial activity.

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Resolution Of Multiple Ssdna Structures In Free Solution Electrophoresis, Eve F. Fabrizio, Ali Nadim, James D. Sterling Oct 2003

Resolution Of Multiple Ssdna Structures In Free Solution Electrophoresis, Eve F. Fabrizio, Ali Nadim, James D. Sterling

Business and Information Technology Faculty Research & Creative Works

By using high concentrations of buffer, electroosmotic flow within uncoated channels of a microfluidic chip was minimized, allowing the free solution electrophoretic separation of DNA. More importantly, because of the ability to efficiently dissipate heat within these channels, field strengths as high as 600 V/cm could be applied with minimal Joule heating (< 2 °C). As a result of the higher field strengths, separations within an 8-cm-long channel were achieved within a few minutes. However, when the electrophoretic separation of single-stranded DNA (ssDNA) less than 22 bases in length was performed, containing the fluorophore Texas Red as an end label, more than the expected single peak was observed at this high electric field. On the other hand, the free solution electrophoresis of a double-stranded DNA (dsDNA) consisting of a random sequence did exhibit the expected single peak. The appearance of these multiple peaks for ssDNA is shown to be dependent upon the base content and sequence of the ssDNA as well as on the chemical structure of the fluorophore used to tag the DNA for detection. Specifically, the peaks can be attributed to different secondary structures that result either from hydrophobic interactions between the DNA bases and an uncharged fluorescent dye or from G-quadruplexes within guanine-rich strands.

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Expressions For Evaluating The Possibility Of Slip At The Liquid-Solid Interface In Open Tube Capillary Electrochromatography, B. A. Grimes, Athanasios I. Liapis Jul 2003

Expressions For Evaluating The Possibility Of Slip At The Liquid-Solid Interface In Open Tube Capillary Electrochromatography, B. A. Grimes, Athanasios I. Liapis

Chemical and Biochemical Engineering Faculty Research & Creative Works

In this work, expressions are constructed and solved that describe the velocity field of electroosmotic flow (EOF) in open tube capillary electrochromatography (CEC) systems when the possibility of having unequal tangential velocities at the liquid-solid interface is considered and a slip condition is employed as a boundary condition for the velocity of the EOF at the capillary wall. the coupled equations of hydrodynamics (momentum balance equation) and electrostatics (Poisson equation) are solved numerically in order to obtain the distribution of the velocity field as well as the value of the volumetric flow rate in the open tube. Also, expressions for …

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Characterization And Optimization Of A Chromatographic Process Based On Ethylenediamine-N,N,N′,N′-Tetra(Methylphosphonic) Acid-Modified Zirconia Particles, Sabyasachi Sarkar, Peter W. Carr, Clayton V. Mcneff, Anuradha Subramanian Jun 2003

Characterization And Optimization Of A Chromatographic Process Based On Ethylenediamine-N,N,N′,N′-Tetra(Methylphosphonic) Acid-Modified Zirconia Particles, Sabyasachi Sarkar, Peter W. Carr, Clayton V. Mcneff, Anuradha Subramanian

Papers in Biotechnology

The primary objective of work was to characterize, optimize and model a chromatographic process based on ethylenediamine-N,N,N′,N′-tetra(methylphosphonic) acid (EDTPA)-modified zirconia particles. Zirconia particles were produced by spray-drying colloidal zirconia. Zirconia spheres produced were further classified, calcined and modified with EDTPA to yield a solid-phase support for use in bio-chromatography (r_PEZ). Specifically, the ability of r_PEZ to selectively bind and enrich IgG, IgA, and IgM from biological fluids was evaluated and demonstrated. To better understand the force of interaction between the IgG and the r_PEZ, the equilibrium disassociation constant (Kd) was determined by static binding isotherms, as a function of temperature …

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Catalytic Wet Oxidation Of Phenol By Hydrogen Peroxide Over Pillared Clay Catalyst, Jing Guo, Muthanna H. Al-Dahhan Jun 2003

Catalytic Wet Oxidation Of Phenol By Hydrogen Peroxide Over Pillared Clay Catalyst, Jing Guo, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

Extrudates of Al-Fe Pillared Clay Catalyst Suitable for Packed-Bed Operations Are Evaluated for Wastewater Treatment Via a Wet Oxidation Process Employing Hydrogen Peroxide as the Oxidant. the Reaction Was Carried Out in a Semibatch Basket Reactor under Rather Mild Conditions. Operational Parameters Were Studied under the Following Conditions: Temperature from 25 to 90 °C, Atmospheric Pressure, Initial Phenol Concentration from 100 to 2000 Ppm of the Liquid Phase, Catalyst Loading from 0 to 10 G/L, and Input H2O2 Concentration from 0.15 to 0.6 Mol/L. under These Conditions, the Al-Fe Pillared Clay Catalyst Achieves a Total Elimination of Phenol and Significant …

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Shape-Controlled Synthesis Of Iron Oxide Nanoparticles, Yangchuan Xing, T. P. Kole, J. L. Katz Jun 2003

Shape-Controlled Synthesis Of Iron Oxide Nanoparticles, Yangchuan Xing, T. P. Kole, J. L. Katz

Chemical and Biochemical Engineering Faculty Research & Creative Works

A study on shape-controlled synthesis of iron oxide nanoparticles was presented. a specially designed counterflow diffusion flame (CDF) reactor was used to synthesize the iron oxide nanoparticles. It was observed that the properties of surface atoms on the nanoparticles are associated with the chemical environments, leading to the preferential growth of a particular crystallographic surface.

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Modeling Of The Fischer-Tropsch Synthesis In Slurry Bubble Column Reactors, Novica Rados, Muthanna H. Al-Dahhan, Milorad P. Dudukovic Apr 2003

Modeling Of The Fischer-Tropsch Synthesis In Slurry Bubble Column Reactors, Novica Rados, Muthanna H. Al-Dahhan, Milorad P. Dudukovic

Chemical and Biochemical Engineering Faculty Research & Creative Works

A Multicomponent One-Dimensional Dynamic Mathematical Model for the Reacting Slurry Systems with a Change in Gas Flow Rate Due to the Chemical Reaction is Developed. a Change in Gas Flow Rate Caused by the Chemical Reaction is Modeled using the overall Gas Mass Balance. Thus, All Relevant Chemical Species Are Included in the Model. Linear First-Order Reaction Kinetics is Considered. the Gas Phase is Modeled using the Two-Bubble Class Hydrodynamic Model. the Interaction between Small and Large Bubbles is Included as the Cross-Flow. Suspension of Liquid and Solids is Assumed to Form a Pseudo Slurry Phase. Back-Mixing in All of …

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Use Of Combinatorial Genetic Libraries To Humanize N-Linked Glycosylation In The Yeast Pichia Pastoris, Byung-Kwon Choi, Piotr Bobrowicz, Robert C. Davidson, Stephen R. Hamilton, David Kung, Huijuan Li, Robert Miele, Juergen Nett, Stefan Wildt, Tillman Gerngross Apr 2003

Use Of Combinatorial Genetic Libraries To Humanize N-Linked Glycosylation In The Yeast Pichia Pastoris, Byung-Kwon Choi, Piotr Bobrowicz, Robert C. Davidson, Stephen R. Hamilton, David Kung, Huijuan Li, Robert Miele, Juergen Nett, Stefan Wildt, Tillman Gerngross

Dartmouth Scholarship

The secretory pathway of Pichia pastoris was genetically re-engineered to perform sequential glycosylation reactions that mimic early processing of N-glycans in humans and other higher mammals. After eliminating nonhuman glycosylation by deleting the initiating alpha-1,6-mannosyltransferase gene from P. pastoris, several combinatorial genetic libraries were constructed to localize active alpha-1,2-mannosidase and human beta-1,2-N-acetylglucosaminyltransferase I (GnTI) in the secretory pathway. First, >32 N-terminal leader sequences of fungal type II membrane proteins were cloned to generate a leader library. Two additional libraries encoding catalytic domains of alpha-1,2-mannosidases and GnTI from mammals, insects, amphibians, worms, and fungi were cloned to generate catalytic domain libraries. …

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Investigation Into The Ultrasound Setting Of Glass Ionomer Cements. Part I. Postulated Modalities, Mark R. Towler, C. M. Crowley, R. G. Hill Apr 2003

Investigation Into The Ultrasound Setting Of Glass Ionomer Cements. Part I. Postulated Modalities, Mark R. Towler, C. M. Crowley, R. G. Hill

Chemical and Biochemical Engineering Faculty Research & Creative Works

Fuji IX glass is a low phosphate, low soda containing glass which has strontia incorporated as a radio-pacifier. This glass material was analyzed during exposure to different durations of ultrasound in an attempt to determine how the glass phase reacts. Cross sections of Fuji IX cement, set both conventionally and by ultrasound, were subsequently compared by microscopy. The resultant data were analyzed in detail.

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Transgenic Non-Human Mammals Expressing Human Coagulation Factor Viii And Von Willebrand Factor, Henryk Lubon, William N. Drohan, William H. Velander Feb 2003

Transgenic Non-Human Mammals Expressing Human Coagulation Factor Viii And Von Willebrand Factor, Henryk Lubon, William N. Drohan, William H. Velander

Papers in Biotechnology

A non-human transgenic mammalian animal, as described above, contains an exogenous double stranded DNA sequence stably integrated into the genome of the animal, which comprises cis-acting regulatory units operably linked to a DNA sequence encoding human Factor VIII protein and a signal peptide, where the cis-acting regulatory units are active in mammary gland cells and the signal peptide is active in directing newly expressed Factor VIII into the milk of the animal. The promoter may be a milk protein promoter such as for whey acidic protein, casein, lactalbumin, or beta-lactoglobulin promoter. The transgenic mammals are preferably farm animals, for example, …

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Pichia Pastoris Fermentation With Mixed-Feeds Of Glycerol And Methanol: Growth Kinetics And Production Improvement, Wenhui Zhang, Karen J. Hywood Potter, Bradley A. Plantz, Vicki L. Schlegel, Leonard A. Smith, Michael M. Meagher Jan 2003

Pichia Pastoris Fermentation With Mixed-Feeds Of Glycerol And Methanol: Growth Kinetics And Production Improvement, Wenhui Zhang, Karen J. Hywood Potter, Bradley A. Plantz, Vicki L. Schlegel, Leonard A. Smith, Michael M. Meagher

Papers in Biochemical Engineering

Fed-batch fermentation of a methanol utilization plus (Mut+) Pichia pastoris strain typically has a growth phase followed by a production phase (induction phase). In the growth phase glycerol is usually used as carbon for cell growth while in the production phase methanol serves as both inducer and carbon source for recombinant protein expression. Some researchers employed a mixed glycerol-methanol feeding strategy during the induction phase to improve production, but growth kinetics on glycerol and methanol and the interaction between them were not reported. The objective of this paper is to optimize the mixed feeding strategy based on growth kinetic studies …

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Sequence Dependent Mobility Of Short Oligonucleotides In Free-Solution Electrophoresis, James D. Sterling, Eve F. Fabrizio, Ali Nadim Jan 2003

Sequence Dependent Mobility Of Short Oligonucleotides In Free-Solution Electrophoresis, James D. Sterling, Eve F. Fabrizio, Ali Nadim

Business and Information Technology Faculty Research & Creative Works

No abstract provided.

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Bubble Dynamics Measurements Using Four-Point Optical Probe, Junli Xue, Muthanna H. Al-Dahhan, M. P. Dudukovic, R. F. Mudde Jan 2003

Bubble Dynamics Measurements Using Four-Point Optical Probe, Junli Xue, Muthanna H. Al-Dahhan, M. P. Dudukovic, R. F. Mudde

Chemical and Biochemical Engineering Faculty Research & Creative Works

By Conducting an Analysis of the Measurement Errors It Was Found that Bubble Dynamics Obtained by Two-Point Probes Can Be Highly Inaccurate. a Four-Point Optical Probe with Appropriate Data Processing Algorithm Has Been Developed So that the Bubble Velocity Vector Can Be Obtained. This Probe Provides Directly the Value of Specific Gas-Liquid Interfacial Area. However, for Obtaining Accurate Values of Bubble Velocity and Size, the Four-Point Probe Must Be Calibrated by a CCD Camera at the Same Conditions. Calibration Columns Have Been Developed for This Purpose Where the Bubble Size and Velocity Can Be Varied to Calibrate the Measurements by …

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Analysis Of Photobioreactors For Culturing High-Value Microalgae And Cyanobacteria Via An Advanced Diagnostic Technique: Carpt, Hu Ping Luo, Abdenour Kemoun, Muthanna H. Al-Dahhan, J. M.Fernández Sevilla, J. L.García Sánchez, F. García Camacho, E. Molina Grima Jan 2003

Analysis Of Photobioreactors For Culturing High-Value Microalgae And Cyanobacteria Via An Advanced Diagnostic Technique: Carpt, Hu Ping Luo, Abdenour Kemoun, Muthanna H. Al-Dahhan, J. M.Fernández Sevilla, J. L.García Sánchez, F. García Camacho, E. Molina Grima

Chemical and Biochemical Engineering Faculty Research & Creative Works

Photosynthetic Algal Cultures Are a Potential Source of Many High-Value Products. in Photobioreactors (PBR), the Availability and the Intensity of the Light, Which Are Affected by the Cells' Movement, Are the Major Factors Controlling the Biomass Productivity. Hydrodynamics, Hence, Play a Significant Role in the Reactor's Performance, as They Determine Not Only the Flow Field, I.e. Liquid Flow and Mixing, Shear Stresses, Etc., But Also the Movements of the Cells. in This Work, Computer-Automated Radioactive Particle Tracking (CARPT) Technique Was Employed to Evaluate its Feasibility for Characterizing PBRs. Liquid Velocity Profiles, Cells' Movement, and the Temporal Irradiance Patterns Obtained by …

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