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Biochemical and Biomolecular Engineering Commons™
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Articles 1 - 17 of 17
Full-Text Articles in Biochemical and Biomolecular Engineering
Cultivation Of Arthrospira Platensis (Spirulina) In Food-Based Industrial Wastewater For High-Yield Phycocyanin Extraction, Pavithira Sathinathan
Cultivation Of Arthrospira Platensis (Spirulina) In Food-Based Industrial Wastewater For High-Yield Phycocyanin Extraction, Pavithira Sathinathan
Student Works (2020-2029)
Arthrospira platensis (Spirulina) is a filamentous cyanobacterium valued for its nutritional profile, particularly the pigment–protein phycocyanin, with potent antioxidant and anti-inflammatory properties. Despite its industrial significance, large-scale commercialization remains constrained by high cultivation costs and inefficient extraction methods. This study investigated the use of food-based industrial wastewater as an alternative nutrient medium for Spirulina cultivation, aiming to reduce production costs, enhance phycocyanin yield, and support sustainable wastewater remediation. Three types of food-based wastewater with different nutrient composition (WW1, WW2, WW3) was diluted with Kosaric medium (1:1) and used for Spirulina cultivation. The highest biomass concentration was achieved in WW2 (1.93 …
Isolated Bacillus Licheniformis Sp01 From Daqing Oilfield In Reducing Chromium(Iii) Crosslinked Polymer Residue And Enhancing Oil Recovery, Xiao Hui
Student Works (2020-2029)
After polymer gel formation, the residual partially hydrolyzed polyacrylamide (HPAM) and the crosslinker (Cr3+) caused plugging and decreased oil recovery. Microorganisms can degrade HPAM to address the issues, but Cr³⁺ inhibits the process and reduces oil recovery. This study explores the underexamined biodegradation of HPAM and improved oil recovery in the presence of Cr³⁺, offering new perspectives for microbial enhanced oil recovery (MEOR) after polymer gel formation. HPAM and crude oil were innovatively used as the sole nitrogen(N) and carbon(C) sources to isolate the best-growing strain from Daqing Oilfield produced water, identified as Bacillus licheniformis SP01. The oil displacement potential …
Torrefaction Of Oil Palm Empty Fruit Bunch Biomass, Mohamad Azri Sukiran
Torrefaction Of Oil Palm Empty Fruit Bunch Biomass, Mohamad Azri Sukiran
Student Works (2020-2029)
Empty fruit bunches (EFB) have great potential to be used as a renewable fuel. Its current low rate of utilization due largely to high moisture content and low calorific value (CV) can be overcome via a thermal pretreatment namely torrefaction. This study investigated the effects of torrefaction temperature, residence time, particle size and moisture content on EFB’s final products distribution and their characteristics in a fixed bed reactor. The solid products (torrefied EFB) obtained were analyzed by various methods such as proximate and ultimate analyses, CV, scanning electron microscopy (SEM), Fourier transform infrared (FTIR), x-ray diffraction (XRD) and thermogravimetric analysis …
Synthesis And Characterization Of Silver Nanoparticles In Lamellar Lyotropic Liquid Crystal, Siti Mariah Mohd Yasin
Synthesis And Characterization Of Silver Nanoparticles In Lamellar Lyotropic Liquid Crystal, Siti Mariah Mohd Yasin
Student Works (2020-2029)
Lamellar lyotropic liquid crystal-silver nanoparticles (LLLC-AgNPs) are synthesized using the in-situ method. The polarizer microscope showed a mosaic-like pattern with fan shape textures. The high resolution transmission electron microscope (HRTEM) confirmed that the AgNPs are spherical in shape with 4 – 12 nm size. Differential scanning calorimetry (DSC) determined the LLLC-AgNPs transition anisotropic phase to the highly ordered isotropic phase at 300 °C, and crystallinity was completed at 350 °C. Thermogravimetric analysis (TGA) verified that the LLLC-AgNPs were completely decomposed at 700 °C. Ultra-violet visible spectroscopy (UV-vis) found the absorption peaks at around 400 nm and proved that the AgNPs …
Process Improvement And Microbial Characterisation Of Biological Nitrogen Removal For Tropical Wastewater With Low Chemical Oxygen Demand/Nitrogen Ratio, How Seow Wah
Student Works (2020-2029)
Malaysia has one of the most established biological nitrogen removal in wastewater treatment plants (WWTPs) among the developing Southeast Asian countries. The current WWTPs’ design and operating guidelines in Malaysia are mainly derived from other developed countries with more experiences in operating biological nitrogen removal. These design guidelines are good practices for WWTPs in the temperate climate region, but may not be optimal for the local wastewater characteristics and tropical climate in Malaysia. The local wastewater industry is keen to improve the design and operation of WWTPs to reduce the energy consumption and operating cost, which helps to foster a …
Fractionation And Characterization Of Extracted Lignin From Oil Palm Empty Fruit Bunch Using Synthesized Deep Eutectic Solvents, Tan Yee Tong
Student Works (2020-2029)
Deep eutectic solvent (DES), a new generation green solvent has been introduced as an alternative pretreatment solvent due to its ability for lignocellulosic biomass fractionation and lignin extraction. Numerous types of compounds can be adopted as DES constituents, which has resulted in abundant variation of DES with varying properties. Selection of the suitable chemical compound category is therefore essential for optimum DES performance. However, investigation on the performance comparison between the different DES categories is lacking. In addition, despite its widely acknowledged effectiveness in biomass fractionation, the properties of DES-fractionated biopolymers, especially lignin are not well understood. Hence, this project …
Alumina Ceramic Membrane Incorporated With Graphene Oxide Frameworks For Protein Recovery, Ishak Noor Fauziyah
Alumina Ceramic Membrane Incorporated With Graphene Oxide Frameworks For Protein Recovery, Ishak Noor Fauziyah
Student Works (2020-2029)
Alumina ceramic membrane is undergoing rapid development and innovation for protein recovery process. However, the limitation of this membrane type is membrane fouling due to the deposition and adsorption of proteins on the surface and pore walls of the membranes. It is crucial to minimize membrane fouling by reducing interactions between protein and the membrane surface via surface modification technique. Thus, the main objective of this research is to develop an alumina membrane with enhanced antifouling property for protein recovery process. An alumina dope consisting of 57 wt.% alumina loading was used to fabricate two alumina membrane configurations; which are …
Waste Cooking Oil Classification Using Artificial Intelligence Technology, Kar Sin Lau
Waste Cooking Oil Classification Using Artificial Intelligence Technology, Kar Sin Lau
Student Works (2020-2029)
Palm oil – one of the most common edible oil consumed in Malaysia. It is because Malaysia is one of the countries which supply palm oil to the global market and it is cheap to obtain for the consumer in Malaysia. Most of the Malaysian consume it via food preparation such as deep-frying and cooking. However, due to widely available for Malaysians, consumers also lacking awareness in dealing after using the edible oil. Most of the household consumers discard excess waste cooking oil (WCO) into sewage and with courtesy, some of them stored them in containers and sell to NGOs. …
Optimization Of Bioethanol Production, Engine Performance And Exhaust Emissions From Manihot Glaziovii And Sweet Sorghum In A Spark Ignition Engine, Sebayang Abdi Hanra
Optimization Of Bioethanol Production, Engine Performance And Exhaust Emissions From Manihot Glaziovii And Sweet Sorghum In A Spark Ignition Engine, Sebayang Abdi Hanra
Student Works (2020-2029)
The ever-increasing fossil fuel consumption over the years is attributable to economic and population growth, which in turn, increases greenhouse gas emissions. Bioethanol is one of the promising solutions to address the depletion of fossil fuels as well as environmental problems. Manihot glaziovii tubers and sweet sorghum grains are inedible feedstocks that can be used for bioethanol production. The carbohydrate content (70%) of these biomasses makes them suitable as feedstocks for bioethanol production. In this study, enzymatic hydrolysis (liquefaction and saccharification) was used to obtain the reduction of sugars. The enzymes α-amylase from Bacillus licheniformis Type XII-A and amyloglucosidase from …
Catalytic Esterification Of Palm-Based Lauric Acid Diethylene Glycol Via Microwave Heating, Abas Noor Azeerah
Catalytic Esterification Of Palm-Based Lauric Acid Diethylene Glycol Via Microwave Heating, Abas Noor Azeerah
Student Works (2020-2029)
Diethylene glycol esters of fatty acids are mixtures of diethylene glycol mono- and di-esters of saturated and unsaturated fatty acids derived from edible oils and fats. The products are produced either by direct esterification of diethylene glycol with fatty acids or by transesterification of diethylene glycol with oils or fats. This study involved in maximizing the conversion of lauric acid to glycol ester via esterification with diethylene glycol, aided by calcined Zn-Mg-Al catalyst in a 250-ml reactor using both conventional and microwave heating. Preliminary catalytic screening involving tin (II) oxalate, Amberlyst-15 and calcined Zn-Mg-Al catalysts in esterification of lauric acid …
Production Of Renewable Hydrocarbon Fuel Via Titanium Oxide-Alumina (Tio2-Al2o3) Catalytic Deoxygenation Reaction, Xian Yih Ooi
Production Of Renewable Hydrocarbon Fuel Via Titanium Oxide-Alumina (Tio2-Al2o3) Catalytic Deoxygenation Reaction, Xian Yih Ooi
Student Works (2020-2029)
The renewable hydrocarbon-like biofuel from biomass is crucial to substitute fossil fuel. Catalytic deoxygenation is a biofuel upgrading process to eliminate the high oxygen content in biofuel which will lead to corrosion, instability and lower heating value problems. The choice of support is significant to provide the maximum acid strength for the hydrogenolysis of C-O bonds. Al2O3 supported catalyst has drawn attention due to the high acidity. However, the high acidity leads to coke deposition, unstable and deactivation of the catalyst. Thus, it is important to develop methods to improve catalyst performance, reduce catalyst coking and enhance catalyst lifetime. Recently, …
Mixed Culture Polyhydroxyalkanaotes (Pha) Production Using Crude Glycerol As Carbon Source, Mohamad Fauzi Ainil Hawa
Mixed Culture Polyhydroxyalkanaotes (Pha) Production Using Crude Glycerol As Carbon Source, Mohamad Fauzi Ainil Hawa
Student Works (2020-2029)
Polyhydroxyalkanoates (PHA) is a biopolymer that has the prospect of becoming a preferred renewable and environmentally friendly resource with various applications including in manufacturing and medicine. Intensive studies have been carried out to find the most practical and economical method for PHA production because current commercial production of PHA is stringent and extremely costly. The aim of this study is to enrich PHA-accumulators with high PHA production capabilities by evaluating the stability of the enrichment process and by observing the effect of organic loading rate (OLR) on the production potential. In this study, PHA-accumulators were cultivated using activated sludge with …
A Microfluidic Paper-Based Analytical Device For Early Diagnosis And Prognosis Of Acute Myocardial Infarction, Lim Wei Yin
A Microfluidic Paper-Based Analytical Device For Early Diagnosis And Prognosis Of Acute Myocardial Infarction, Lim Wei Yin
Student Works (2020-2029)
Acute myocardial infarction (AMI), also termed as heart attack, is often clinically screened by blood test through the measurement of cardiac biomarkers (i.e., troponin (T or I), creatine kinase (CK−MB) and myoglobin) and electrocardiography (ECG) changes which are presented to the hospital. However, the early diagnosis of AMI is challenging because the detection of biomarkers such as troponin (T or I) and CK−MB are only reliable at the intermediate and later stages (> 6 hours after chest pain onset), during myocardial injury and myocardial infarction. On the other hand, myoglobin often been used as early biomarker which is detectable within …
Response Surface Optimization Of Crystal Violet Decolorization By Pellets Of Pyncnoporus Sanguineus., Siti Suhana Sulaiman
Response Surface Optimization Of Crystal Violet Decolorization By Pellets Of Pyncnoporus Sanguineus., Siti Suhana Sulaiman
Student Works (2010-2019)
Ligninolytic enzymes of white-rot fungi have broad specificities and have been implicated in transformation and mineralization of organo-pollutant with chemical structure similar to lignin e.g. synthetic dyes. This study was carried out to optimize selected parameters for decolorization of Crystal violet by Pycnoporus sanguineus in shake flasks. The parameters studied were initial dye concentration (ppm), agitation speed (rpm) and process time. They were optimized using response surface methodology (RSM). Subsequent to the optimization, the decolorization process was scaled up in stirred tank reactor (STR). Effect of different geometry of impellers on the decolorization process was studied. Curved blade and angled …
Effect Of Selected Physical And Chemical Parameters On Crystal Violet Decolorization By Immobilized Laccase., Nur Atiqah Ismail
Effect Of Selected Physical And Chemical Parameters On Crystal Violet Decolorization By Immobilized Laccase., Nur Atiqah Ismail
Student Works (2010-2019)
Synthetic dyes are used for coloration of various materials like textiles, leather and food. Wastewater from dyeing industries constitutes a threat to the environment as many synthetic dyes are often toxic, mutagenic and carcinogenic. Besides, light absorption is hindered by the dyes run-off creating problems to photosynthetic aquatic plants and algae. Biodegradation of waste dyestuff with enzymes provides a viable alternative compared to the often expensive physical/chemical methods. Laccase from Trametes versicolor was chosen in this study as it exhibits low substrate specificity and high ability to oxidize many pollutants which suggested its potential in wastewater treatment and bioremediation. The …
Isolation, Screening And Characterisation Of Lactic Acid Bacteria Isolated From Local Food Sources, Ahmad Faris Mohd. Adnan
Isolation, Screening And Characterisation Of Lactic Acid Bacteria Isolated From Local Food Sources, Ahmad Faris Mohd. Adnan
Student Works (2000-2009)
126 bacterial isolates were isolated from several local food sources. Out of these, 55 isolates were subsequently identified as lactic acid bacteria i.e. they were gram-positive and catalase negative. These 55 isolates were put through gel plug test to distinguish between homofermentative and heterofermentative isolates. 16 of the 55 lactic acid bacteria isolates were found to be homofermentative. From the 16 homofermentative isolates, seven were chosen on the basis of origin and sugars used in the enrichment process for preliminary physiological characterisation. Several conditions were chosen such as variable temperature, lactic acid tolerance, NaCl concentration and initial pH These serve …
Production Of Polyhydroaxyalkanoate (Pha) From Palm Oil By Pseudomonas Oleovorans And Indigenous Bacterial Isolate, Pseudomonas Sp., Zazali Alias
Student Works (2000-2009)
In this study, the yield of biomass and polyhydroxyalkanoate (PHA) of Pseudomonas oleovorans grown in batch and fed-batch cultivations were compared. When oleic acid (QA) (0.5% w/v) was used as carbon source, a significant increase in biomass.and PHA content were observed through fedbatch cultivation. The biomass and PHA content obtained from batch cultivation were 1.45±0.19 g/I and 2.50±0.77 % cell dry weight (CDW) respectively, while in the fed-batch cultivation, the biomass and PHA content increased significantly to 3.35±0.78 g/I and 32.72±0.30 %CDW respectively. When saponified palm olein (SPO) (0.5% w/v) was used as a carbon source, the batch cultivation yielded …