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Articles 151 - 180 of 332

Full-Text Articles in Chemical Engineering

Transgenic Fibrinogen, William H. Velander Aug 2006

Transgenic Fibrinogen, William H. Velander

Department of Chemical and Biomolecular Engineering: Patents

A transgenic, non-human mammalian animal is capable of expressing a heterologous gene for human or other recombinant physiologically functional fibrinogen holoprotein or individual subunit chain polypeptides thereof or a modified or fusion fibrinogen in mammary glands of the animals and secreting the expressed product into a body fluid. Methodology employing such a mammal yields recombinant physiologically functional fibrinogens, subunit chain polypeptides thereof, and modified or fusion fibrinogens.

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Efficient, Thermally Stable, Second Order Nonlinear Optical Response In Organic Hybrid Covalent/Ionic Self-Assembled Films, Kevin E. Van Cott May 2006

Efficient, Thermally Stable, Second Order Nonlinear Optical Response In Organic Hybrid Covalent/Ionic Self-Assembled Films, Kevin E. Van Cott

Papers in Biomolecular Engineering

A covalent/electrostatic layer-by-layer self-assembly method was used to achieve polar ordering of a water soluble, reactive dye in the fabrication of nonlinear optical (NLO) films. We observed a quadratic relationship between the second harmonic intensity I2 and bilayer number for all films made with Procion Brown MX-GRN, demonstrating that the polar ordering of the chromophores is consistent in each successive bilayer. As the ionic strength of the dye deposition solution was increased to 0.5 M NaCl, the of the films increased by approximately 250% to 50 × 10-9 esu, with a corresponding average chromophore tilt angle of 38. This was …

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Non-Isothermal Reaction-Diffusion Systems With Thermodynamically Coupled Heat And Mass Transfer, Yaşar Demirel May 2006

Non-Isothermal Reaction-Diffusion Systems With Thermodynamically Coupled Heat And Mass Transfer, Yaşar Demirel

Papers in Analytical Chemistry

Non-isothermal reaction-diffusion (RD) systems control the behavior of many transport and rate processes in physical, chemical, and biological systems. A considerable work has been published on mathematically coupled nonlinear differential equations of RD systems by neglecting the possible thermodynamic couplings among heat and mass fluxes, and reaction velocities. Here, the thermodynamic coupling refers that a flux occurs without its primary thermodynamic driving force, which may be gradient of temperature, or chemical potential, or reaction affinity. This study presents the modeling equations of non-isothermal RD systems with coupled heat and mass fluxes excluding the coupling of chemical reactions using the linear …

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Means Of Seeding And Metallizing Polyimide, Ravi F. Saraf Apr 2006

Means Of Seeding And Metallizing Polyimide, Ravi F. Saraf

Department of Chemical and Biomolecular Engineering: Patents

A method to selectively metallize polyimide with an all electroless process.

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A Quantitative Model Of Error Accumulation During Pcr Amplification, Elsje Pienaar, M. Theron, M. Nelson, Hendrik J. Viljoen Apr 2006

A Quantitative Model Of Error Accumulation During Pcr Amplification, Elsje Pienaar, M. Theron, M. Nelson, Hendrik J. Viljoen

Hendrik J. Viljoen Publications

The amplification of target DNA by the polymerase chain reaction (PCR) produces copies which may contain errors. Two sources of errors are associated with the PCR process: (1) editing errors that occur during DNA polymerase-catalyzed enzymatic copying and (2) errors due to DNA thermal damage. In this study a quantitative model of error frequencies is proposed and the role of reaction conditions is investigated. The errors which are ascribed to the polymerase depend on the efficiency of its editing function as well as the reaction conditions; specifically the temperature and the dNTP pool composition. Thermally induced errors stem mostly from …

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Retrofit Of Distillation Columns Using Thermodynamic Analysis, Yaşar Demirel Apr 2006

Retrofit Of Distillation Columns Using Thermodynamic Analysis, Yaşar Demirel

Papers in Physical Properties

Thermodynamic analysis provides the column grand composite curves and exergy loss profiles, which are becoming readily available for a converged distillation column simulation. For example, the Aspen Plus simulator performs the thermodynamic analysis through its Column–Targeting tool for rigorous column calculations. This study uses the column grand composite curves and the exergy loss profiles obtained from Aspen Plus to assess the performance of the existing distillation columns, and reduce the costs of operation by appropriate retrofits in a methanol plant. Effectiveness of the retrofits is also assessed by means of thermodynamics and economics. The methanol plant utilizes two distillation columns …

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Use Of A Modified Zirconia Support In The Separation Of Immunoproteins, Anuradha Subramanian, Sabyasachi Sarkar Mar 2006

Use Of A Modified Zirconia Support In The Separation Of Immunoproteins, Anuradha Subramanian, Sabyasachi Sarkar

Papers in Biotechnology

Zirconia beads (25–38 μm in diameter) were modified with N,N,N′,N′-ethylenediaminetetramethylenephosphonic acid to generate a zirconia based pseudoaffinity support, further referred to as r_PEZ. The influence of pH, salt concentration and temperature on the binding of human immunoglobulin G (hIgG) to r_PEZ was studied. Temperature had no significant impact on the maximum binding capacity (Qmax), and the equilibrium-binding constant (Kd), whereas pH and the salt concentration had a noticeable impact on both Qmax and Kd. The Qmax value of 55 mg hIgG/ml of bead was obtained at a pH of 5.5 and found to decrease with an increase of pH. The …

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A Comparative Study Of Monoclonal Antibodies (Mabs) Purified From Cell Culture Supernatant On Edtpa-Modified Zirconia Beads And Protein A-Hyper D Support, Anuradha Subramanian, Blanca Martinez, Jill Holm, Peter W. Carr, Clayton V. Mcneff Mar 2006

A Comparative Study Of Monoclonal Antibodies (Mabs) Purified From Cell Culture Supernatant On Edtpa-Modified Zirconia Beads And Protein A-Hyper D Support, Anuradha Subramanian, Blanca Martinez, Jill Holm, Peter W. Carr, Clayton V. Mcneff

Papers in Biotechnology

Colloidal zirconia was spray dried to yield zirconia particles, which were further modified with N, N, N0, N0- Ethylenediamine tetra methylenephosphonic acid (EDTPA) to yield a sup-port for use in bioseparations. EDTPA modified zirconia particles will be further referred to as, r_PEZ. Cell culture supernatants rich in monoclonal antibody (Mab) subtypes IgG1, IgG2a, IgG2b, and IgG3 were chromatographed on a r_PEZ column, and on a protein A-hyper D col-umn that was purchased commercially. All Mab subtypes bound to r_PEZ and process yields in the range of 88 to 99% were obtained. The purity of the Mab products were ascertained by …

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Discover Magazine - Cyborg Bacteria, Ravi F. Saraf, Vikas Berry Feb 2006

Discover Magazine - Cyborg Bacteria, Ravi F. Saraf, Vikas Berry

Department of Chemical and Biomolecular Engineering: News Releases

In a society that has goldplated everything from hubcaps to teeth, it was perhaps inevitable that someone would find a way to add some bling to bacteria.

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Engineering Opens Year With New Lab Celebrations Jan 2006

Engineering Opens Year With New Lab Celebrations

Department of Chemical and Biomolecular Engineering: News Releases

The Department of Chemical and Biomolecular Engineering hosted rt ribborx-cuttlng ceremony Jan. 6 to mark the lab's move from the first floor of Othmer HaIl to the basement.

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Engineering Opens Year With New Lab Celebrations Jan 2006

Engineering Opens Year With New Lab Celebrations

Department of Chemical and Biomolecular Engineering: News Releases

The Department of Chemical and Biomolecular Engineering hosted a ribbon-cutting ceremony Jan. 6 to mark the lab's move from the first floor of Othmer Hall to the basement. The new lab is designed to mimic a factory so students can use equipment similar to that found in factories and research labs, said William Velander, chair of Chemical and Biomolecular Engineering. The lab features enhanced utility hookups necessary for chemical processing.

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Transgenic Non-Human Mammals Producing Fibrinogen In Their Milk, William H. Velander Jan 2006

Transgenic Non-Human Mammals Producing Fibrinogen In Their Milk, William H. Velander

Department of Chemical and Biomolecular Engineering: Patents

A transgenic, non-human mammalian animal is capable of expressing a heterologous gene for human or other recombinant physiologically functional fibrinogen holoprotein or individual subunit chain polypeptides thereof or a modified or fusion fibrinogen in mammary glands of the animals and secreting the expressed product into a body fluid. Methodology employing such a mammal yields recombinant physiologically functional fibrinogens, subunit chain polypeptides thereof, and modified or fusion fibrinogens.

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Purification And Scale-Up Of A Recombinant Heavy Chain Fragment C Of Botulinum Neurotoxin Serotype E In Pichia Pastoris Gs115, Michael P. Dux, Rick Barent, Jayanta Sinha, Mark Gouthro, Todd Swanson, Ardis Barthuli, Mehmet Inan, John T. Ross, Leonard A. Smith, Theresa J. Smith, Robert Webb, Bonnie Loveless, Ian Henderson, Michael M. Meagher Jan 2006

Purification And Scale-Up Of A Recombinant Heavy Chain Fragment C Of Botulinum Neurotoxin Serotype E In Pichia Pastoris Gs115, Michael P. Dux, Rick Barent, Jayanta Sinha, Mark Gouthro, Todd Swanson, Ardis Barthuli, Mehmet Inan, John T. Ross, Leonard A. Smith, Theresa J. Smith, Robert Webb, Bonnie Loveless, Ian Henderson, Michael M. Meagher

Michael Meagher Publications

A recombinant C-terminus heavy chain fragment from botulinum neurotoxin serotype E (BoNT/E) is proposed as a vaccine against the serotype E neurotoxin. This fragment, rBoNTE(Hc), was produced intracellular in Pichia pastoris GS115 by a three-step fermentation process, i.e., glycerol batch phase and a glycerol fed-batch phase to achieve high cell densities, followed by a methanol fed-batch induction phase. The rBoNTE(Hc) protein was purified from the soluble fraction of cell lysates using three ion-exchange chromatography steps (SP Sepharose Fast Flow, Q Sepharose Fast Flow, Sp Sepharose High Performance) and polished with a hydrophobic charge induction chromatography step …

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Royal Society Of Chemistry - Gold-Plated Bacteria, Ravi F. Saraf, Vikas Berry Oct 2005

Royal Society Of Chemistry - Gold-Plated Bacteria, Ravi F. Saraf, Vikas Berry

Department of Chemical and Biomolecular Engineering: News Releases

No Abstract

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Nature - Nanotechnology-Live Wire, Ravi F. Saraf, Vikas Berry Oct 2005

Nature - Nanotechnology-Live Wire, Ravi F. Saraf, Vikas Berry

Department of Chemical and Biomolecular Engineering: News Releases

No Abstact.

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Phase Stability Analysis Using Interval Newton Method With Nrtl Model, Hatice Gecegormez, Yaşar Demirel Oct 2005

Phase Stability Analysis Using Interval Newton Method With Nrtl Model, Hatice Gecegormez, Yaşar Demirel

Papers in Fluid Mechanics

The Gibbs energy minimization using activity coefficient models and nonlinear equation solution techniques are commonly applied for phase stability problems. However, dependence on the initial estimates and multiple solutions for these highly nonlinear equations are common drawbacks for some of the conventional approaches. We have used interval Newton method with the local composition model of NRTL for the phase stability analysis of 10 binary systems and 2 ternary systems at various feed compositions to locate all the stationary points. Results indicate that the interval Newton method is reliable and efficient.

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Enhancement Of Protein Secretion In Pichia Pastoris By Overexpression Of Protein Disulfide Isomerase, Mehmet İnan, Dinesh Aryasomayajula, Jayanta Sinha, Michael M. Meagher Sep 2005

Enhancement Of Protein Secretion In Pichia Pastoris By Overexpression Of Protein Disulfide Isomerase, Mehmet İnan, Dinesh Aryasomayajula, Jayanta Sinha, Michael M. Meagher

Papers in Biochemical Engineering

A potential vaccine candidate, Necator americanus secretory protein (Na-ASP1), against hookworm infections, has been expressed in Pichia pastoris. Na- ASP1, a 45 kDa protein containing 20 cysteines, was directed outside the cell by fusing the protein to the preprosequence of the α-mating factor of Saccharomyces cerevisiae. Most of the protein produced by single copy clones was secreted outside the cell. However, increasing gene copy number of Na-ASP1 protein in P. pastoris saturated secretory capacity and therefore, decreased the amount of secreted protein in clones harboring multiple copies of Na-ASP1 gene. Overexpression of the …

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Cgmp Recombinant Fix For Iv And Oral Hemophilia B Therapy, William H. Velander Principal Investigator, Abramson Stephan Lead Investigator, Cooper Julian Lead Investigator, William Dernell Key Person, Mark Manning Lead Investigator, Michael Meagher Lead Investigator, Paul Mohanan Lead Investigator, Timothy Nichols Lead Investigator, Todd Swaanson Key Person, Kevin E. Van Cott Sep 2005

Cgmp Recombinant Fix For Iv And Oral Hemophilia B Therapy, William H. Velander Principal Investigator, Abramson Stephan Lead Investigator, Cooper Julian Lead Investigator, William Dernell Key Person, Mark Manning Lead Investigator, Michael Meagher Lead Investigator, Paul Mohanan Lead Investigator, Timothy Nichols Lead Investigator, Todd Swaanson Key Person, Kevin E. Van Cott

Department of Chemical and Biomolecular Engineering: Funded Proposals

Three specific aims are proposed: Specific Aim # 1. Process engineer and scale-up the recovery and purification of transgenic recombinant human Factor IX. The University of Nebraska-Lincoln Biological Process Development Facility will complete process development and scale-up, and produce clinical grade materials for preclinical studies. The endpoint is a proposed final product specification to help facilitate transfer to current Good Manufacturing Practices compliant production of clinical grade material to support an Investigational New Drug filing with the United States Food and Drug Administration (FDA) leading to clinical trials. Specific Aim #2. Characterize and formulate transgenic recombinant human Factor IX for …

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Under Graduate Course Description Sep 2005

Under Graduate Course Description

Department of Chemical and Biomolecular Engineering: Courses

No abstract provided.

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Crosslinked Chitosan: Its Physical Properties And The Effects Of Matrix Stiffness On Chondrocyte Cell Morphology And Proliferation, Anuradha Subramanian, Hsin-Yi Lin Aug 2005

Crosslinked Chitosan: Its Physical Properties And The Effects Of Matrix Stiffness On Chondrocyte Cell Morphology And Proliferation, Anuradha Subramanian, Hsin-Yi Lin

Papers in Biotechnology

Chitosan [<(1-4)-2 amino-2-deoxy-d-glucose], the natural polyaminosaccharide derived from N-deacetylation of chitin [<(1-4)-2 acetamide-2-deoxy-d-glucose], has been shown to possess attractive biological and cell interactive properties. Recently chitosan and chitosan analogs have also been shown to support the growth and continued function of chondrocytes. In the present study, chitosan substrates are crosslinked with a func-tional diepoxide (1,4 butanediol diglycidyl ether) to alter its mechanical property, and the viability and proliferation of the canine articular chondrocytes seeded on the crosslinked surface are further assayed. Of interest is the impact of substrate stiffness on the growth and proliferation of articular canine chondrocytes. Cross linked scaffolds were also sub-jected to degradation by chitosanase to examine the impact of cross linking on enzyme- assisted degradation. The hydrophilicity and compression modulus of the crosslinked sur-faces were measured via contact-angle measurements and compression tests, respec-tively. Scanning electron microscopy (SEM) and fluorescent staining were used to ob-serve the proliferation and morphology of chondrocyte cells on noncrosslinked and crosslinked surfaces. The crosslinked chitosan was found to be nontoxic to chondrocytes and more hydrophilic. Its compression modulus and stiffness increased, which may im-prove the scaffold resistance to wear and in vivo shrinkage once implanted. The in-creased stiffness also seemed to serve as an additional mechanical stimulus to promote chondrocyte growth and proliferation. The cell morphology on crosslinked scaffolds seen by SEM and fluorescent stain was the typical chondrocytic rounded shape. The method proposed provides a nontoxic way to increase the mechanical strength of the chitosan scaffolds.

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Purification And Scale-Up Of A Recombinant Heavy Chain Fragment C Of Botulinum Neurotoxin Serotype E In Pichia Pastoris Gs115, Michael P. Dux, Rick Barent, Jayanta Sinha, Mark Gouthro, Todd Swanson, Ardis Barthuli, Mehmet İnan, John T. Ross, Leonard A. Smith, Theresa J. Smith, Robert Webb, Bonnie Loveless, Ian Henderson, Michael M. Meagher Aug 2005

Purification And Scale-Up Of A Recombinant Heavy Chain Fragment C Of Botulinum Neurotoxin Serotype E In Pichia Pastoris Gs115, Michael P. Dux, Rick Barent, Jayanta Sinha, Mark Gouthro, Todd Swanson, Ardis Barthuli, Mehmet İnan, John T. Ross, Leonard A. Smith, Theresa J. Smith, Robert Webb, Bonnie Loveless, Ian Henderson, Michael M. Meagher

Papers in Biochemical Engineering

A recombinant C-terminus heavy chain fragment from Botulinum neurotoxin serotype E (BoNT/E) is proposed as a vaccine against the serotype E neurotoxin. This fragment, rBoNTE(Hc), was produced intracellular in Pichia pastoris GS115 by a three-step fermentation process, i.e., glycerol batch phase and a glycerol fed-batch phase to achieve high cell densities, followed by a methanol fed-batch induction phase. The rBoNTE(Hc) protein was purified from the soluble fraction of cell lysates using three ion-exchange chromatography steps (SP Sepharose Fast Flow, Q Sepharose Fast Flow, Sp Sepharose High Performance) and polished with a hydrophobic charge induction chromatography step (MEP HyperCel). Method development …

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The Phosphoglycolate Phosphatase Gene And The Mutation In The Phosphoglycolate Phosphatase-Deficient Mutant (Pgp1-1) Of Chlamydomonas Reinhardtii, Kensaku Suzuki, Hidenobu Uchida, Tarlan G. Mamedov Jul 2005

The Phosphoglycolate Phosphatase Gene And The Mutation In The Phosphoglycolate Phosphatase-Deficient Mutant (Pgp1-1) Of Chlamydomonas Reinhardtii, Kensaku Suzuki, Hidenobu Uchida, Tarlan G. Mamedov

Papers in Biochemical Engineering

The sequences of the phosphoglycolate phosphatase (PGPase) gene Pgp1 and the 5′-upstream region from Chlamydomonas reinhardtii wildtype 2137 and the pgp1-1 mutant N142 that lacks the activity of PGPase (PGP1) were determined. The comparison revealed the alteration of a G to A at position 98 relative to the start codon. This destroyed the “GT” splice donor site at the beginning of the first intron of this gene, resulting in an extension of the first exon to 49 translatable codons followed by a stop codon, containing the codons corresponding to whole transit peptide for the chloroplast stroma and the first four …

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Uv-Vis -Infrared Optical And Afm Study Of Spin-Cast Chitosan Films, W H. Nosal, D. W. Thompson, Sabyasachi Sarkar, Anuradha Subramanian, John A. Woollam, L Yan Jun 2005

Uv-Vis -Infrared Optical And Afm Study Of Spin-Cast Chitosan Films, W H. Nosal, D. W. Thompson, Sabyasachi Sarkar, Anuradha Subramanian, John A. Woollam, L Yan

Papers in Biotechnology

Optical properties OS spin-cast chitosan films have been determined in the infrared, visible, and ultraviolet region or the spectrum ~tsing spectroscopic ellipsometry. Optical constants for the Vvis-near IR spectra from 130 to 1700 nm were determined using Cauchy dispersion forms com-bined with Lorentzian oscillator models in the absorptive shorler wavelength regions. Infrared ndex of refraction and extinction coefficients from 750 to 4000cm-' were determined using ellip-sometric data fits to dispersion models based on harmonic oscillators. This modeling determined that optical onisotropy was present and measurable over all wavelength regions of ellipsomctric data. To obtain information on the micro- and nano-scale …

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Stability Of Transport And Rate Processes [Review], Yaşar Demirel Jun 2005

Stability Of Transport And Rate Processes [Review], Yaşar Demirel

Papers in Thermal Mechanics

About fifty years ago, the Turing instability demonstrated that even simple reaction-diffusion systems might lead to spatial order and differentiation, while the Rayleigh-Bénard instability showed that the maintenance of nonequilibrium might be the source of order in fluids subjected to a thermodynamic force above a critical value. Therefore, distance from global equilibrium in the form of magnitude of a thermodynamic force emerges as another constraint of stability; some systems may enhance perturbations, and evolve to highly organized states called the dissipative structures after a critical distance on the thermodynamic branch. Although the kinetics and transport coefficients represent short-range interactions, chemical …

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Chromatographic Purification Of Mabs With Non-Affinity Supports, Anuradha Subramanian Jun 2005

Chromatographic Purification Of Mabs With Non-Affinity Supports, Anuradha Subramanian

Papers in Biotechnology

The introduction of new protein-based therapeutics such as monoclonal antibodies (MAbs), MAb-based vaccines, growth factors and plasma proteins implies the need to study, characterize, and purify. The separation step is likely to be a bottleneck and cost-effective technology will be needed to rectify it. The currently prevalent matrices for chromatographic separation of immunoglobulins (Igs) are based on Protein A or its recombinant versions (Protein G). They display excellent selectivity and specificity, but are expensive. A Protein A matrix costs $8,000 to 12,000 per L-resin. The typical production column volume is 100 L. The million-dollar matrix is far more expensive than …

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Modeling Of Immunoglobulin Uptake By N,N,N′,N′-Ethylenediaminetetramethylenephosphonic Acid-Modified Zirconia Particles Under Static And Dynamic Conditions, Sabyasachi Sarkar, Anuradha Subramanian May 2005

Modeling Of Immunoglobulin Uptake By N,N,N′,N′-Ethylenediaminetetramethylenephosphonic Acid-Modified Zirconia Particles Under Static And Dynamic Conditions, Sabyasachi Sarkar, Anuradha Subramanian

Papers in Biotechnology

A matrix developed from N,N,N′,N′-ethylenediaminetetramethylenephosphonic acid-modified zirconia beads (further referred to as r_PEZ); 25–38 μm in diameter and with a pore size of 22 ± 3 nm, was utilized for the separation of immunoglobulins (Igs). r_PEZ has been shown to bind to various Igs originating from a wide variety of species. To understand the mechanisms controlling the uptake of Igs by r_PEZ, static protein uptake experiments were carried out. The protein uptake profiles were further modeled with a kinetic rate constant model. Individual studies were undertaken for human immunoglobulin A, G and M (HIgA, HIgG and HIgM). The kinetic rate …

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The Use Of Confocal Laser Scanning Microscopy To Study The Transport Of Biomacromolecules In A Macroporous Support, Anuradha Subramanian, Jennifer Hommerding Apr 2005

The Use Of Confocal Laser Scanning Microscopy To Study The Transport Of Biomacromolecules In A Macroporous Support, Anuradha Subramanian, Jennifer Hommerding

Papers in Biotechnology

Large-pore materials or supports resembling polymer conduits are used as packing material in chromatographic operations. Our ongoing research has shown that, when modified with peptides or ligands, chitosan beads that are 800 m in diameter and have 3.5% solids can be used as matrices in bioseparations. The goal of the present study is to evaluate the transport properties of biomolecules in the modified chitosan beaded matrices. Batch uptake experiments with fluorescently tagged pure human IgG, human IgA and human IgM were conducted to visualize the distribution of binding sites throughout the bead as well as to evaluate restrictions to diffusion, …

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Identification Of The Mass Transfer Mechanisms Involved In The Transport Of Human Immunoglobulin - G In N,N,N',N' -Ethylnediaminetetramenthylenephosphonic Acid Modified Zirconia, Sabyasachi Sarkar, Perer W. Carr, Anuradha Subramanian Apr 2005

Identification Of The Mass Transfer Mechanisms Involved In The Transport Of Human Immunoglobulin - G In N,N,N',N' -Ethylnediaminetetramenthylenephosphonic Acid Modified Zirconia, Sabyasachi Sarkar, Perer W. Carr, Anuradha Subramanian

Papers in Biotechnology

Zirconia particles modified with N,N,N′,N′-ethylenediaminetetramethylenephosphonic acid (EDTPA), further referred to as r_PEZ, were studied as a support material for use in chromatography. Our previous studies have demonstrated the utility of r_PEZ in the separation of immunoglobulins from biological fluids. In the present study we sought to understand the underlying factors and identify the rate-limiting mechanisms that govern the transport of biomolecules in r_PEZ. Pulse injection techniques were used to elucidate the individual mass transfer parameters. Elution profiles obtained under retained and unretained conditions were approximated by the Gaussian equation and the corresponding HETP contributions were estimated. The dependence of …

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Identification And Expression Analysis Of Two Inorganic C-And N-Responsive Genes Encoding Novel And Distinct Molecular Forms Of Eukaryotic Phosphoenolpyruvate Carboxylase In The Green Microalga Chlamydomonas Reinhardtii, Tarlan G. Mamedov, Eric R. Moellering, Raymond Chollet Mar 2005

Identification And Expression Analysis Of Two Inorganic C-And N-Responsive Genes Encoding Novel And Distinct Molecular Forms Of Eukaryotic Phosphoenolpyruvate Carboxylase In The Green Microalga Chlamydomonas Reinhardtii, Tarlan G. Mamedov, Eric R. Moellering, Raymond Chollet

Papers in Biochemical Engineering

Phosphoenolpyruvate carboxylase (PEPC [Ppc]) has been previously purified and characterized in biochemical and immunological terms from two green microalgae, Chlamydomonas reinhardtii and Selenastrum minutum. The findings indicate that these algae possess at least two distinct PEPC enzyme-forms, homotetrameric Class1 and heteromeric Class-2 that differ significantly from each other and their plant and prokaryotic counterparts. Surprisingly, however, green-algal PEPC has been unexplored to date in molecular terms. This study reports the molecular cloning of the two Ppc genes in C. reinhardtii (CrPpc1, CrPpc2), each of which is transcribed in vivo and encodes a fully active, recombinant PEPC that lacks the regulatory, …

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Crystallization And Preliminary X-Ray Analysis Of Na-Asp-1, A Multi-Domain Pathogenesis-Related-1 Protein From The Human Hookworm Parasite Necator Americanus, Mehmet İnan, Oluwatoyin A. Asojo, Alex Lucas, Rick Barent, Jicai Huang, Brad Plantz, Amber Swanson, Mark Gouthro, Michael M. Meagher, Peter J. Hotez Mar 2005

Crystallization And Preliminary X-Ray Analysis Of Na-Asp-1, A Multi-Domain Pathogenesis-Related-1 Protein From The Human Hookworm Parasite Necator Americanus, Mehmet İnan, Oluwatoyin A. Asojo, Alex Lucas, Rick Barent, Jicai Huang, Brad Plantz, Amber Swanson, Mark Gouthro, Michael M. Meagher, Peter J. Hotez

Papers in Biochemical Engineering

Human hookworm infection is a major cause of anemia and malnutrition in the developing world. In an effort to control hookworm infection, the Human Hookworm Vaccine Initiative has identified candidate vaccine antigens from the infective larval stage (L3) of the parasite, including a family of pathogenesis related- 1 (PR-1) proteins known as the ancylostoma-secreted proteins (ASPs). The functions of the ASPs are unknown. In addition, it is unclear why some ASPs have one while others have multiple PR-1 domains. There are no known structures of a multi-domain ASP and in an effort to remedy this situation, recombinant Na …

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