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University of Nebraska - Lincoln

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Articles 691 - 720 of 1371

Full-Text Articles in Bioresource and Agricultural Engineering

Controlled De-Cross-Linking And Disentanglement Of Feather Keratin For Fiber Preparation Via A Novel Process, Helan Xu, Yiqi Yang Jan 2014

Controlled De-Cross-Linking And Disentanglement Of Feather Keratin For Fiber Preparation Via A Novel Process, Helan Xu, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Pure protein fibers were fabricated from chicken feathers via a potentially green process. In the last several decades, efforts have been made to produce keratin-based industrial products, especially fibers. However, the methods of producing keratin fibers directly from chicken feathers could not be repeated. In this research, protein fibers from chicken feathers were developed using chemicals that could be either derived from renewable resources or facilely recycled. Backbones of keratin were preserved after cleavage of disulfide bonds using cysteine. Sodium dodecyl sulfate (SDS) was applied to dissolve keratin for spinning. Increasing SDS concentration intensified the ordered conformation of keratin, first …

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Water-Stable Three-Dimensional Ultrafine Fibrous Scaffolds From Keratin For Cartilage Tissue Engineering, Helan Xu, Shaobo Cai, Lan Xu, Yiqi Yang Jan 2014

Water-Stable Three-Dimensional Ultrafine Fibrous Scaffolds From Keratin For Cartilage Tissue Engineering, Helan Xu, Shaobo Cai, Lan Xu, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Intrinsically water-stable scaffolds composed of ultrafine keratin fibers oriented randomly and evenly in three dimensions were electrospun for cartilage tissue engineering. Keratin has been recognized as a biomaterial that could substantially support the growth and development of multiple cell lines. Besides, three-dimensional (3D) ultrafine fibrous structures were preferred in tissue engineering due to their structural similarity to native extracellular matrices in soft tissues. Recently, we have developed a nontraditional approach to developing 3D fibrous scaffolds from alcohol-soluble corn protein, zein, and verified their structural advantages in tissue engineering. However, keratin with highly cross-linked molecular structures could not be readily dissolved …

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Intrinsically Water-Stable Keratin Nanoparticles And Their In Vivo Biodistribution For Targeted Delivery, Helan Xu, Zhen Shi, Narendra Reddy, Yiqi Yang Jan 2014

Intrinsically Water-Stable Keratin Nanoparticles And Their In Vivo Biodistribution For Targeted Delivery, Helan Xu, Zhen Shi, Narendra Reddy, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Highly water-stable nanoparticles of around 70 nm and capable of distributing with high uptake in certain organs of mice were developed from feather keratin. Nanoparticles could provide novel veterinary diagnostics and therapeutics to boost efficiency in identification and treatment of livestock diseases to improve protein supply and ensure safety and quality of food. Nanoparticles could penetrate easily into cells and small capillaries, surpass detection of the immune system, and reach targeted organs because of their nanoscale sizes. Proteins with positive and negative charges and hydrophobic domains enable loading of various types of drugs and, hence, are advantageous over synthetic polymers …

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Development And Testing Of An In-Stream Phosphorus Cycling Model For The Soil And Water Assessment Tool, Michael J. White, Daniel E. Storm, Aaron R. Mittelstet, Philip R. Busteed, Brian E. Haggard, Colleen Rossi Jan 2014

Development And Testing Of An In-Stream Phosphorus Cycling Model For The Soil And Water Assessment Tool, Michael J. White, Daniel E. Storm, Aaron R. Mittelstet, Philip R. Busteed, Brian E. Haggard, Colleen Rossi

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The Soil and Water Assessment Tool is widely used to predict the fate and transport of phosphorus (P) from the landscape through streams and rivers. The current in-stream P submodel may not be suitable for many stream systems, particularly those dominated by attached algae and those affected by point sources. In this research, we developed an alternative submodel based on the equilibrium P concentration concept coupled with a particulate scour and deposition model. This submodel was integrated with the SWAT model and applied to the Illinois River Watershed in Oklahoma, a basin influenced by waste water treatment plant discharges and …

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The Hydraulic Conductivity Structure Of Gravel-Dominated Vadose Zones Within Alluvial Floodplains, Ronald B. Miller, Derek M. Heeren, Garey A. Fox, Todd Halihan, Daniel E. Storm, Aaron R. Mittelstet Jan 2014

The Hydraulic Conductivity Structure Of Gravel-Dominated Vadose Zones Within Alluvial Floodplains, Ronald B. Miller, Derek M. Heeren, Garey A. Fox, Todd Halihan, Daniel E. Storm, Aaron R. Mittelstet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The floodplains of many gravel-bed streams have a general stratigraphy that consists of a layer of topsoil covering gravel-dominated subsoil. Previous research has demonstrated that this stratigraphy can facilitate preferential groundwater flow through focused linear features, such as paleochannels, or gravelly regions within the vadose zone. These areas within the floodplain vadose zone may provide a route for interactions between the floodplain surface and alluvial groundwater, effectively extending the hyporheic zone across the floodplain during high stream stage. The objective of this research was to assess the structure and scale of texture heterogeneity within the vadose zone within the gravel …

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Simulation Supported Estimation Of End-To-End Transmission Parameters In Non-Viral Gene Delivery, Beata J. Wysocki, Tadeusz A. Wysocki, Timothy M. Martin, Angela K. Pannier Jan 2014

Simulation Supported Estimation Of End-To-End Transmission Parameters In Non-Viral Gene Delivery, Beata J. Wysocki, Tadeusz A. Wysocki, Timothy M. Martin, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Communications, in general, involve delivery of information from a source to a sink. At nano-scale, an example of a man-made communications involving interfacing with biological systems at intra-cellular level is non-viral gene delivery. From a telecommunications engineering perspective, important end-to-end parameters of such a system are: the endto- end delay, system capacity, and packet loss rate. There are neither known methods to estimate those parameters theoretically nor they are ready available from standard measurements. The paper provides estimates for those parameters based on the simulation of non-viral gene delivery system based on the queuing theory. The simulator used has been …

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Alginate/Silica Hybrid Materials For Immobilization Of Green Microalgae Chlorella Vulgaris For Cellbased Sensor Arrays, Angela K. Pannier, Ulrich Soltmann, Bettina Soltmann, Rolf Altenburger, Mechthild Schmitt-Jansen Jan 2014

Alginate/Silica Hybrid Materials For Immobilization Of Green Microalgae Chlorella Vulgaris For Cellbased Sensor Arrays, Angela K. Pannier, Ulrich Soltmann, Bettina Soltmann, Rolf Altenburger, Mechthild Schmitt-Jansen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Thin layers and patterned dot arrays of sodium alginate containing living microalgal cells were deposited onto glass carriers which were subsequently gelled using amino-functionalized silica sol to obtain reinforced alginate hydrogels. The resulting alginate/silica hybrid materials showed improved stability in salt-containing solutions compared to alginate gels gelled by traditional methods using Ca2+-ions. Cell arrays were patterned by printing nanolitre-scale drops of sodium alginate/cell suspension using a noncontact micro-dosage system which allows the printing of solutions of high viscosity. Cultures of the green microalga Chlorella vulgaris were immobilized within the newly developed alginate/silica hydrogels in order to demonstrate the …

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In Vitro Development Of Preimplantation Porcine Embryos Using Alginate Hydrogels As A Three-Dimensional Extracellular Matrix, Catherine Sargus-Patino, Elane C. Wright, Sarah A. Plautz, Jeremy R. Miles, Jeff L. Vallet, Angela K. Pannier Jan 2014

In Vitro Development Of Preimplantation Porcine Embryos Using Alginate Hydrogels As A Three-Dimensional Extracellular Matrix, Catherine Sargus-Patino, Elane C. Wright, Sarah A. Plautz, Jeremy R. Miles, Jeff L. Vallet, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Between Days 10 and 12 of gestation, porcine embryos undergo a dramatic morphological change, known as elongation, with a corresponding increase in oestrogen production that triggers maternal recognition of pregnancy. Elongation deficiencies contribute to embryonic loss, but exact mechanisms of elongation are poorly understood due to the lack of an effective in vitro culture system. Our objective was to use alginate hydrogels as three-dimensional scaffolds that can mechanically support the in vitro development of preimplantation porcine embryos. White cross-bred gilts were bred at oestrus (Day 0) to Duroc boars and embryos were recovered on Days 9, 10 or 11 of …

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Long-Term Activity Of Biohybrid Coatings Of Atrazine-Degrading Bacteria Pseudomonas Sp. Adp, Angela K. Pannier, T. Lehrer, M. Vogel, U. Soltmann, H. Bottcher, S. Tarre, M. Green, J. Raff, K. Pollmann Jan 2014

Long-Term Activity Of Biohybrid Coatings Of Atrazine-Degrading Bacteria Pseudomonas Sp. Adp, Angela K. Pannier, T. Lehrer, M. Vogel, U. Soltmann, H. Bottcher, S. Tarre, M. Green, J. Raff, K. Pollmann

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The atrazine-degrading bacterial strain Pseudomonas sp. ADP was immobilized by the sol–gel process within thin silica layers coated onto water-retaining carrier materials (expanded clay pellets and scoria). The performance of the obtained biohybrid material has been investigated concerning long-term activity under non-growth conditions. Experiments were run in phosphate buffer containing atrazine (20 mg l-1) as the sole source of carbon and nitrogen. Even after one year of consecutive batch tests, P. ADP immobilized onto expanded clay pellets showed a high atrazine degradation activity. In the course of long-term batch experiments, the average amount of removed atrazine was about …

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Nutrient Concentrations Of Runoff As Affected By The Diameter Of Unconsolidated Material From Feedlot Surfaces, John E. Gilley, Gregory Boone, David B. Marx Jan 2014

Nutrient Concentrations Of Runoff As Affected By The Diameter Of Unconsolidated Material From Feedlot Surfaces, John E. Gilley, Gregory Boone, David B. Marx

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Beef cattle feedlots contain unconsolidated material that accumulates on the feedlot surface during a feeding cycle. This study was conducted to measure the effects of varying diameters of unconsolidated surface material and varying flow rates on nutrient concentrations in runoff. Unconsolidated surface material with an average diameter of 4.76, 9.53, 19.1, or 47.5 mm and a composite sample with a 15.2 mm mean diameter were placed within 0.75 m wide × 4.0 m long plot areas. Flow was then introduced at the top of the plots in successive increments, and runoff samples for water quality analyses were obtained. Particle diameter …

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Dynamic Analysis Of Dna Nanoparticle Immobilization To Model Biomaterial Substrates Using Combinatorial Spectroscopic Ellipsometry And Quartz Crystal Microbalance With Dissipation, Tadas Kasputis, Alex Pieper, Mathias Schubert, Angela K. Pannier Jan 2014

Dynamic Analysis Of Dna Nanoparticle Immobilization To Model Biomaterial Substrates Using Combinatorial Spectroscopic Ellipsometry And Quartz Crystal Microbalance With Dissipation, Tadas Kasputis, Alex Pieper, Mathias Schubert, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Gene expression within cells can be altered through gene delivery approaches, which have tremendous potential for gene therapy, tissue engineering, and diagnostics. Substrate-mediated gene delivery describes the delivery of plasmid DNA or DNA complexed with nonviral vectors to cells from a surface, with the DNA immobilized to a substrate through specific or nonspecific interactions. In this work, DNA-nanoparticle (DNA–NP) adsorption to substrates is evaluated using combinatorial, in situ spectroscopic ellipsometry and quartz crystal microbalance with dissipation (SE/QCM-D), to evaluate the basic dynamic processes involved in the adsorption and immobilization of DNA–NP complexes to substrates. The concentration of DNA–NP solutions influences …

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Seed Tape Effects On Corn Emergence Under Greenhouse Conditions, Ramarao Venkatesh, Peter R. Thomison, Colette K. Gabriel, Mark A. Bennett, Elaine M. Grassbaugh, Matthew D. Kleinhenz, Scott A. Shearer, Santosh Pitla Jan 2014

Seed Tape Effects On Corn Emergence Under Greenhouse Conditions, Ramarao Venkatesh, Peter R. Thomison, Colette K. Gabriel, Mark A. Bennett, Elaine M. Grassbaugh, Matthew D. Kleinhenz, Scott A. Shearer, Santosh Pitla

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Seed tape has recently received attention as an alternative planting system for smallholder farmers in underdeveloped regions of South America, Africa, China, and India (Mateus, 2014). Seed companies are also developing seed-tape planting systems for germplasm evaluations (Deppermann et al., 2013). Although seed tape has been promoted as a method for ensuring uniform seed spacing and plant density of small-seeded flowers, herbs, and vegetables (Chancellor, 1969), little or no information is available on the use of seed tape for larger-seeded row crops and its effect on crop emergence. The objective of this study was to compare the emergence of corn …

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2014 Nebraska Water Monitoring Programs Report, Marty Link, Ryan Chapman Jan 2014

2014 Nebraska Water Monitoring Programs Report, Marty Link, Ryan Chapman

Nebraska Department of Environmental Quality: Reports

The Nebraska Department of Environmental Quality (NDEQ) is charged with monitoring, assessing, and to the extent possible, managing the state’s water resources. The purpose of this work is to protect and maintain high quality water and encourage or execute activities to improve poor water quality. Monitoring is done on nearly 17,000 miles of flowing rivers and streams, more than 134,000 acres of surface water in lakes and reservoirs, as well as the vast storage of groundwater in Nebraska’s aquifers.

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2014 Nebraska Groundwater Quality Monitoring Report Jan 2014

2014 Nebraska Groundwater Quality Monitoring Report

Nebraska Department of Environmental Quality: Reports

The 2001 Nebraska Legislature passed LB329 (Neb. Rev. Stat. §46-1304) which, in part, directed the Nebraska Department of Environmental Quality (NDEQ) to report on groundwater quality monitoring in Nebraska. Reports have been issued annually since December 2001. The text of the statute applicable to this report follows: “The Department of Environmental Quality shall prepare a report outlining the extent of ground water quality monitoring conducted by natural resources districts during the preceding calendar year. The department shall analyze the data collected for the purpose of determining whether or not ground water quality is degrading or improving and shall present the …

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Soybean Yield, Evapotranspiration, Water Productivity, And Soil Water Extraction Response To Subsurface Drip Irrigation And Fertigation, Suat Irmak, James E. Specht, Lameck O. Odhiambo, J. M. Rees, K. G. Cassman Jan 2014

Soybean Yield, Evapotranspiration, Water Productivity, And Soil Water Extraction Response To Subsurface Drip Irrigation And Fertigation, Suat Irmak, James E. Specht, Lameck O. Odhiambo, J. M. Rees, K. G. Cassman

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Soybean [Glycine max (L.) Merr.] yield, irrigation water use efficiency (IWUE), crop water use efficiency (CWUE), evapotranspiration water use efficiency (ETWUE), and soil water extraction response to eleven treatments of full, limited, or delayed irrigation versus a rainfed control were investigated using a subsurface drip irrigation (SDI) system at a research site in south-central Nebraska. The SDI system laterals were 0.40 m deep in every other row middle of 0.76 m spaced plant rows. Actual evapotranspiration (ETa) was quantified in all treatments and used to schedule irrigation events on a 100% ETa replacement basis in all but …

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Combined Qcm-D/Ge As A Tool To Characterize Stimuli-Responsive Swelling Of And Protein Adsorption On Polymer Brushes Grafted Onto 3d-Nanostructures, Meike Koenig, Tadas Kasputis, Daniel Schmidt, Keith B. Rodenhausen Jr., Klaus-Jochen Eichhorn, Angela K. Pannier, Mathias Schubert, Manfred Stamm, Petra Uhlmann Jan 2014

Combined Qcm-D/Ge As A Tool To Characterize Stimuli-Responsive Swelling Of And Protein Adsorption On Polymer Brushes Grafted Onto 3d-Nanostructures, Meike Koenig, Tadas Kasputis, Daniel Schmidt, Keith B. Rodenhausen Jr., Klaus-Jochen Eichhorn, Angela K. Pannier, Mathias Schubert, Manfred Stamm, Petra Uhlmann

Department of Agricultural and Biological Systems Engineering: Faculty Publications

A combined setup of quartz crystal microbalance and generalized ellipsometry can be used to comprehensively investigate complex functional coatings comprising stimuli-responsive polymer brushes and 3D nanostructures in a dynamic, noninvasive in situ measurement. While the quartz crystal microbalance detects the overall change in areal mass, for instance, during a swelling or adsorption process, the generalized ellipsometry data can be evaluated in terms of a layered model to distinguish between processes occurring within the intercolumnar space or on top of the anisotropic nanocolumns. Silicon films with anisotropic nanocolumnar morphology were prepared by the glancing angle deposition technique and further functionalized by …

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Parallel Synthesis Of Poly(Amino Ether)-Templated Plasmonic Nanoparticles For Transgene Delivery, James Ramos, Thrimoorthy Potta, Olivia Scheideler, Kaushal Rege Jan 2014

Parallel Synthesis Of Poly(Amino Ether)-Templated Plasmonic Nanoparticles For Transgene Delivery, James Ramos, Thrimoorthy Potta, Olivia Scheideler, Kaushal Rege

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Plasmonic nanoparticles have been increasingly investigated for numerous applications in medicine, sensing, and catalysis. In particular, gold nanoparticles have been investigated for separations, sensing, drug/nucleic acid delivery, and bioimaging. In addition, silver nanoparticles demonstrate antibacterial activity, resulting in potential application in treatments against microbial infections, burns, diabetic skin ulcers, and medical devices. Here, we describe the facile, parallel synthesis of both gold and silver nanoparticles using a small set of poly(amino ethers), or PAEs, derived from linear polyamines, under ambient conditions and in absence of additional reagents. The kinetics of nanoparticle formation were dependent on PAE concentration and chemical composition. …

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Surface-Enhanced Raman Spectral Biomarkers Correlate With Ankle Brachial Index And Characterize Leg Muscle Biochemical Composition Of Patients With Peripheral Arterial Disease, Kim Cluff, Abby M. Kelly, Panagiotis Koutakis, Xiang N. He, Xi Huang, Yongfeng Lu, Iraklis I. Pipinos, George P. Casale, Jeyamkondan Subbiah Jan 2014

Surface-Enhanced Raman Spectral Biomarkers Correlate With Ankle Brachial Index And Characterize Leg Muscle Biochemical Composition Of Patients With Peripheral Arterial Disease, Kim Cluff, Abby M. Kelly, Panagiotis Koutakis, Xiang N. He, Xi Huang, Yongfeng Lu, Iraklis I. Pipinos, George P. Casale, Jeyamkondan Subbiah

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Peripheral arterial disease (PAD) is characterized by atherosclerotic blockages of the arteries supplying the lower extremities, which cause a progressive accumulation of ischemic injury to the skeletal muscles of the lower limbs. This injury includes altered metabolic processes, damaged organelles, and compromised bioenergetics in the affected muscles. The objective of this study was to explore the association of Raman spectral signatures of muscle biochemistry with the severity of atherosclerosis in the legs as determined by the Ankle Brachial Index (ABI) and clinical presentation. We collected muscle biopsies from the gastrocnemius (calf muscle) of five patients with clinically diagnosed claudication, five …

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The Biophysical Link Between Climate, Water, And Vegetation In Bioenergy Agro-Ecosystems, Justin E. Bagley, Sarah C. Davis, Matei Georgesu, Mir Zaman Hussain, Jesse Miller, Stephen W. Nesbitt, Andy Vanloocke, Carl J. Bernacchi Jan 2014

The Biophysical Link Between Climate, Water, And Vegetation In Bioenergy Agro-Ecosystems, Justin E. Bagley, Sarah C. Davis, Matei Georgesu, Mir Zaman Hussain, Jesse Miller, Stephen W. Nesbitt, Andy Vanloocke, Carl J. Bernacchi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Land use change for bioenergy feedstocks is likely to intensify as energy demand rises simultaneously with increased pressure to minimize greenhouse gas emissions. Initial assessments of the impact of adopting bioenergy crops as a significant energy source have largely focused on the potential for bioenergy agroecosystems to provide global-scale climate regulating ecosystem services via biogeochemical processes. Such as those processes associated with carbon uptake, conversion, and storage that have the potential to reduce global greenhouse gas emissions (GHG). However, the expansion of bioenergy crops can also lead to direct biophysical impacts on climate through water regulating services. Perturbations of processes …

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Divergence And Flow Direction As Indicators Of Subsurface Heterogeneity And Stage-Dependent Storage In Alluvial Floodplains, Derek M. Heeren, Garey A. Fox, A. K. Fox, Daniel E. Storm, Ronald B. Miller, Aaron R. Mittelstet Jan 2014

Divergence And Flow Direction As Indicators Of Subsurface Heterogeneity And Stage-Dependent Storage In Alluvial Floodplains, Derek M. Heeren, Garey A. Fox, A. K. Fox, Daniel E. Storm, Ronald B. Miller, Aaron R. Mittelstet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Assuming homogeneity in alluvial aquifers is convenient, but limits our ability to accurately predict stream-aquifer interactions. Research is needed on (i) identifying the presence of focused, as opposed to diffuse, groundwater discharge/recharge to streams and (ii) the magnitude and role of large-scale bank and transient storage in alluvial floodplains relative to changes in stream stage. The objective of this research was to document and quantify the effect of stage-dependent aquifer heterogeneity and bank storage relative to changes in stream stage using groundwater flow divergence and direction. Monitoring was performed in alluvial floodplains adjacent to the Barren Fork Creek and Honey …

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Use Of Controller Area Network (Can) Data To Determine Field Efficiencies Of Agricultural Machinery, Santosh Pitla, N. Lin, Joe D. Luck, Scott A. Shearer Jan 2014

Use Of Controller Area Network (Can) Data To Determine Field Efficiencies Of Agricultural Machinery, Santosh Pitla, N. Lin, Joe D. Luck, Scott A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Controller Area Network (CAN) bus is used on current agricultural equipment for in-vehicle communication among multiple microcontrollers. In this study, CAN data was collected from the tractor diagnostic port during three field operations: anhydrous ammonia (NH3) application, field cultivation, and planting. Specifically, CAN messages corresponding to engine fuel use rate were filtered, decoded, and analyzed. High fuel use rates were recorded from the tractor when implements were engaged in the soil compared to when the implements were lifted out of the ground at the end of the passes or, when the tractor was turning and idling. Threshold fuel …

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Fate Of Antimicrobials And Antimicrobial Resistance Genes In Simulated Swine Manure Storage, Stacey R. Joy, Xu Li, Daniel D. Snow, John Gilley, Bryan L. Woodbury, Shannon L. Bartelt-Hunt Jan 2014

Fate Of Antimicrobials And Antimicrobial Resistance Genes In Simulated Swine Manure Storage, Stacey R. Joy, Xu Li, Daniel D. Snow, John Gilley, Bryan L. Woodbury, Shannon L. Bartelt-Hunt

School of Natural Resources: Faculty Publications

The behavior of three antibiotics (bacitracin, chlortetracycline, and tylosin) and two classes of antibiotic resistance genes (ARGs), tet and erm, were monitored in swine manure slurry under anaerobic conditions. First-order decay rates were determined for each antibiotic with half-lives ranging from1 day (chlortetracycline) to 10 days (tylosin). ARGs were monitored in the swine manure slurry, and losses of approximately 1 to 3 orders of magnitude in relative abundance were observed during the 40 day storage period. First-order degradation profiles were observed for chlortetracycline and its corresponding resistance genes, tet(X) and tet(Q). Tylosin was degraded to approximately 10% …

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Elementary Implantable Force Sensor For Smart Orthopaedic Implants, Rebecca A. Wachs, David Ellstein, John Drazan, Colleen P. Healey, Richard L. Uhl, Kenneth A. Connor, Eric H. Ledet Dec 2013

Elementary Implantable Force Sensor For Smart Orthopaedic Implants, Rebecca A. Wachs, David Ellstein, John Drazan, Colleen P. Healey, Richard L. Uhl, Kenneth A. Connor, Eric H. Ledet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Implementing implantable sensors which are robust enough to maintain long term functionality inside the body remains a significant challenge. The ideal implantable sensing system is one which is simple and robust; free from batteries, telemetry, and complex electronics. We have developed an elementary implantable sensor for orthopaedic smart implants. The sensor requires no telemetry and no batteries to communicate wirelessly. It has no on-board signal conditioning electronics. The sensor itself has no electrical connections and thus does not require a hermetic package. The sensor is an elementary L-C resonator which can function as a simple force transducer by using a …

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Yield And Crop Water Use Efficiency Response Of Drought-Tolerant And Conventional Maize Hybrids Under Different Irrigation Regimes, Plant Populations And Climate Gradients, Ali T. Mohammed Oct 2013

Yield And Crop Water Use Efficiency Response Of Drought-Tolerant And Conventional Maize Hybrids Under Different Irrigation Regimes, Plant Populations And Climate Gradients, Ali T. Mohammed

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Water scarcity in many regions of the world, including Nebraska, is imposing increasing pressure on water demands in agriculture to meet future food and fiber demand of rapidly growing world populations. One of the viable alternatives to counter this challenge is cultivating new drought-tolerant hybrids/varieties/cultivars. Recently, new drought-tolerant maize (Zea mays L.) hybrids have been developed by major seed companies. However, it is essential to evaluate the performance of these hybrids, in terms of grain yield and crop water use (actual evapotranspiration) in various climatic and soil conditions under large production settings. Research was conducted at four locations across the …

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Narrow Grass Hedge Effects On Nutrient Transport Following Swine Slurry Application, John E. Gilley, Shannon L. Bartelt-Hunt, Seth J. Lamb, Xu Li, David B. Marx, Daniel D. Snow, David B. Parker, Brian L. Woodbury Jul 2013

Narrow Grass Hedge Effects On Nutrient Transport Following Swine Slurry Application, John E. Gilley, Shannon L. Bartelt-Hunt, Seth J. Lamb, Xu Li, David B. Marx, Daniel D. Snow, David B. Parker, Brian L. Woodbury

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The effectiveness of a narrow grass hedge in reducing runoff nutrient loads following swine slurry application was examined. Slurry was applied to 0.75 m wide by 4.0 m long plots established on an Aksarben silty clay loam soil located in southeast Nebraska. Manure treatments consisted of no manure application and manure application to meet the 1, 2, or 3 year nitrogen (N) requirements for corn. Runoff water quality was measured during three 30 min simulated rainfall events. The grass hedge did not significantly reduce runoff nutrient transport after the swine slurry, which contained relatively small amounts of manure, was applied. …

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Finite Element Modeling Of Phosphorus Leaching Through Floodplain Soils Dominated By Preferential Flow Pathways, Ryan P. Freiberger, Derek M. Heeren, Garey A. Fox Jul 2013

Finite Element Modeling Of Phosphorus Leaching Through Floodplain Soils Dominated By Preferential Flow Pathways, Ryan P. Freiberger, Derek M. Heeren, Garey A. Fox

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Phosphorus is a critical nutrient in soils, providing both positive and negative effects to different systems. While optimum crop growth requires a range of P above 0.2 mg/L, preventing surface water enrichment generally requires P to be below 0.03 mg/L. Proper application and control of phosphorus is important to increase farming efficiency and to protect freshwater systems from toxic algal growth. While the movement of phosphorus through many soil types has been well-documented, the presence of highly conductive, gravel outcrops and macropores in soil can have a significant, poorly-documented effect on phosphorus movement. In the Ozark ecoregion, for example, the …

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Zein: Novel Natural Polymer For Nanoparticle- And Film-Mediated Gene Delivery, Jessica D. Taylor Jul 2013

Zein: Novel Natural Polymer For Nanoparticle- And Film-Mediated Gene Delivery, Jessica D. Taylor

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Gene delivery, the introduction of DNA into cells, is applicable to gene therapy, DNA vaccination, functional genomics and diagnostics, tissue engineering, and drug-eluting medical devices. Particulates incorporating DNA are promising vehicles for gene delivery and overcome some of the barriers that hinder successful gene transfer, with the ability to protect DNA and provide for controlled, localized, and sustained release and transfection. Furthermore, innovative new gene delivery strategies that incorporate DNA particulates or complexes within films or coatings for devices and scaffolds could further provide for controlled and sustained transfection at the site of implant. Zein, a hydrophobic protein from corn, …

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Nanowell-Trapped Charged Ligand-Bearing Nanoparticle Surfaces – A Novel Method Of Enhancing Flow-Resistant Cell Adhesion, Phat L. Tran, Jessica R. Gamboa, Katherine E. Mccracken, Mark R. Riley, Marvin J. Slepian, Jeong-Yeal Yoon Jul 2013

Nanowell-Trapped Charged Ligand-Bearing Nanoparticle Surfaces – A Novel Method Of Enhancing Flow-Resistant Cell Adhesion, Phat L. Tran, Jessica R. Gamboa, Katherine E. Mccracken, Mark R. Riley, Marvin J. Slepian, Jeong-Yeal Yoon

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Assuring cell adhesion to an underlying biomaterial surface is vital in implant device design and tissue engineering, particularly under circumstances where cells are subjected to potential detachment from overriding fl uid fl ow. Cell–substrate adhesion is a highly regulated process involving the interplay of mechanical properties, surface topographic features, electrostatic charge, and biochemical mechanisms. At the nanoscale level, the physical properties of the underlying substrate are of particular importance in cell adhesion. Conventionally, natural, pro-adhesive, and often thrombogenic, protein biomaterials are frequently utilized to facilitate adhesion. In the present study, nanofabrication techniques are utilized to enhance the biological functionality of …

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Impact Of Measurement Scale On Infiltration And Phosphorus Leaching In Ozark Floodplains, Derek M. Heeren, Garey A. Fox, Daniel E. Storm, Brian E. Haggard, Chad J. Penn, Todd Halihan Jul 2013

Impact Of Measurement Scale On Infiltration And Phosphorus Leaching In Ozark Floodplains, Derek M. Heeren, Garey A. Fox, Daniel E. Storm, Brian E. Haggard, Chad J. Penn, Todd Halihan

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Increased nutrient loads have resulted in several adverse impacts on surface water quality, including excessive algal growth, fish kills, and drinking water taste and odor issues across the United States and especially in the Ozark ecoregion of northeastern Oklahoma and northwestern Arkansas. The significance of this problem has been highlighted by litigation, with one case even reaching the U.S. Supreme Court (Arkansas et al. v. Oklahoma et al., 503 U.S. 91) which required the upstream state to meet downstream water quality standards. The overarching objective of this line of research was to characterize phosphorus leaching to alluvial aquifers in the …

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Application Of Isothermal Calorimetry To The Study Of Phosphorus Sorption Onto Soils In A Flow-Through System, Chad J. Penn, Derek M. Heeren, Garey A. Fox Jul 2013

Application Of Isothermal Calorimetry To The Study Of Phosphorus Sorption Onto Soils In A Flow-Through System, Chad J. Penn, Derek M. Heeren, Garey A. Fox

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The degree, mechanisms, and kinetics of phosphorus (P) sorption onto soils can have a significant influence on leaching losses of P from soil. The objectives of this study were to measure the impact of retention time (RT) on P sorption in a flow-through system intended to simulate downward movement of a P solution through two different riparian soils, and determine if isothermal titration calorimetry (ITC) can provide useful information reflective of flow-through results. Topsoil from two riparian/alluvial soils was sampled and characterized for P concentrations and parameters related to P sorption. Flow-through P sorption experiments were conducted in order to …

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