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Articles 1 - 4 of 4
Full-Text Articles in Systems and Integrative Engineering
High-Throughput Robotic Ethanol Inhibition Assays For Engineered Thermophilic Biofuel Strains, Kevin He, Daniel Olson, Marybeth Maloney, Anthony Lanahan
High-Throughput Robotic Ethanol Inhibition Assays For Engineered Thermophilic Biofuel Strains, Kevin He, Daniel Olson, Marybeth Maloney, Anthony Lanahan
Wetterhahn Science Symposium Posters
Ethanol stress assays are commonly used to evaluate microbial tolerance, metabolic adaptation, and fermentation performance. However, manual liquid handling introduces variability across replicate wells and small-volume pipetting steps, limiting reproducibility and throughput. This study developed an automated OT-2 robotic workflow to generate replicated ethanol concentration gradients for high-throughput inhibition assays in engineered thermophilic biofuel strains. Kinetic plate-reader measurements were used to quantify ethanol-dependent growth responses under anaerobic fermentation conditions. The reasearch question is: How do engineered thermophilic biofuel strains differ in ethanol-dependent growth inhibition under anaerobic fermentation conditions, and can automated robotic assays improve the reproducibility of these measurements? Can …
Thermophysical Properties Of Aqueous Amine Salts For Closed Air Cabin Revitalization, Mahmud R. Yusuf, Isabela Flores, Ben Hines, Li Hua Zhang, Seth Christa, Brooks D. Rabideau, Kevin West, Breanna Dobyns, James Davis
Thermophysical Properties Of Aqueous Amine Salts For Closed Air Cabin Revitalization, Mahmud R. Yusuf, Isabela Flores, Ben Hines, Li Hua Zhang, Seth Christa, Brooks D. Rabideau, Kevin West, Breanna Dobyns, James Davis
Shelby Hall Graduate Research Forum Posters
Carbon dioxide (CO2) capture plays a crucial role in closed cabin air revitalization, however current CO2 capture technologies pose several issues. Traditional amine solutions, such as monoethanolamine, are commonly used in CO2 capture on the International Space Station but face drawbacks including high volatility, thermal degradation, and unpleasant odor. To overcome these challenges, amine salt solutions have emerged as promising alternatives. Amine salt solutions have reduced environmental impact, lower corrosion potential, and improved CO2 loading capacity, making them a more efficient and sustainable replacement. Knowing these physical properties are critical for modeling and optimizing a scrubbing process that employs these …
The Subject Librarian Newsletter, Engineering And Computer Science, Fall 2017, Buenaventura "Ven" Basco
The Subject Librarian Newsletter, Engineering And Computer Science, Fall 2017, Buenaventura "Ven" Basco
Libraries' Newsletters
No abstract provided.
Beyond Biobricks: Synthesizing Synergistic Biochemical Systems From The Bottom-Up, Mark A. Bedau
Beyond Biobricks: Synthesizing Synergistic Biochemical Systems From The Bottom-Up, Mark A. Bedau
Systems Science Friday Noon Seminar Series
Engineers who attempt to discover and optimize the behavior of complex biochemical systems face a dauntingly difficult task. This is especially true if the systems are governed by multiple qualitative and quantitative variables that have non-linear response functions and that interact synergistically. The synthetic biology community has responded to this difficulty by promoting the use of "standard biological parts" called "BioBricks", which are supposed to make biology into traditional engineering and enable engineers to "program living organisms in the same way a computer scientists can program a computer". But the BioBricks research program faces daunting hurdles, because the nonlinearity and …