Biomedical Engineering and Bioengineering Commons^™

Detection Of Leukocytes Stained With Acridine Orange Using Unique Spectral Features Acquired From An Image-Based Spectrometer, Courtney J. Hunter

Biomedical Engineering Undergraduate Honors Theses

A leukocyte differential count can be used to diagnosis a myriad blood disorders, such as infections, allergies, and efficacy of disease treatments. In recent years, attention has been focused on developing point-of-care (POC) systems to provide this test in global health settings. Acridine orange (AO) is an amphipathic, vital dye that intercalates leukocyte nucleic acids and acidic vesicles. It has been utilized by POC systems to identify the three main leukocyte subtypes: granulocytes, monocytes, and lymphocytes. Subtypes of leukocytes can be characterized using a fluorescence microscope, where the AO has a 450 nm excitation wavelength and has two peak emission …

Characterization Of The Response Of Trpv4 To Chemical Stimulation, Jacob V. Schluns

Biomedical Engineering Undergraduate Honors Theses

Traumatic Brain Injury (TBI) is a source of acute and chronic health issues for many patients. One of the components of the brain’s response to injury is astrogliosis, in which astrocytes that normally function to repair the brain instead form scar tissue that halts repair processes. Transient Receptor Potential Vanilloid Type 4 (TRPV4) is a trans-membrane calcium channel involved in astrogliosis. Through Fura-2AM based calcium imaging, the base activity of this channel in mouse astrocyte cells was recorded. The cells were then subjected to TRPV4 agonist and antagonist stimulation and their subsequent activity levels were recorded. The data showed that …

Development Of Peritoneal Microbubble Oxygenation As An Extrapulmonary Treatment For Hypoxia, Nathan Legband

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

Patients affected by a respiratory disease or injury experience a substantially impaired respiratory system and as a consequence are unable to obtain a sufficient amount of oxygen. Hypoxia can quickly result in developing permanent tissue damage or death. Currently, the medical methods of treating hypoxia are mechanical ventilation or extracorporeal membrane oxygenation. However, these treatments are ineffective in certain cases and possess significant additional risks including barotrauma, infection, hemorrhage, and thrombosis.

The extrapulmonary method of peritoneal oxygenation has been investigated by other research groups as a potential alternative to providing supplemental oxygen in hypoxic animals. In peritoneal oxygenation, the peritoneum, …

Design And Validation Of An In Vivo Long-Term Attachment Capsule Robot, Wanchuan Xie

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

The invention of capsule endoscopy (CE) made the non-invasive monitoring of the entire small bowel possible and became the primary means for diagnosing small bowel pathology. In the last decade, capsule robots have been transformed from diagnostic devices into a widely studied biomedical platform with the potential for active locomotion, drug delivery and therapeutic capabilities. To perform accurate on-site drug release and therapy, it is necessary for a capsule robot to be able to attach to the intestinal tissue and maintain its position long-term. Design challenges derive from the task of long-term mucosal adhesion which requires firm, quick-response attachment without …

Biplane Fluoroscopy For Hindfoot Motion Analysis During Gait: A Model-Based Evaluation, Janelle A. Cross, Ben Mchenry, Robert C. Molthen, Emily Exten, Taly Gilat-Schmidt, Gerald F. Harris

Biomedical Engineering Faculty Research and Publications

The purpose of this study was to quantify the accuracy and precision of a biplane fluoroscopy system for model-based tracking of in vivo hindfoot motion during over-ground gait. Gait was simulated by manually manipulating a cadaver foot specimen through a biplane fluoroscopy system attached to a walkway. Three 1.6-mm diameter steel beads were implanted into the specimen to provide marker-based tracking measurements for comparison to model-based tracking. A CT scan was acquired to define a gold standard of implanted bead positions and to create 3D models for model-based tracking. Static and dynamic trials manipulating the specimen through the capture volume …

Optimizing A Standard Fasting Time For 2-Nbdg Uptake Studies In Murine Breast Cancers, Andrew C. Briley

Biomedical Engineering Undergraduate Honors Theses

Recently, there has been a larger use of 2-NBDG, a fluorescent glucose analog, to study glucose uptake in different cell types. These cell types have ranged anywhere from bacteria to human cancer cells. However, there has yet to be a standard procedure and practice for using 2-NBDG. In this study, our goal is to create a standard fasting time for the cells before introducing 2-NBDG to them. This study uses 4T07 cells, a murine breast cancer cell line, to help optimize this fasting time. These cells were fasted at different time points in order to find the ideal fasting time. …

Effect Of Storage Temperature And Time On Lyophilized Water Bark Extract's Biological Activity, Jillian Schneider

Biological and Agricultural Engineering Undergraduate Honors Theses

Sweetgum bark extract has been known to show biological activities such as antimicrobial and antioxidant capabilities. The storage capacity of the extract, however, was unknown and previously thought to diminish over time. However, upon experimentation, the freeze-dried sweetgum bark extract showed no signs that storage time or storage temperature had any significant effect on the biological activities. There was no significant difference across storage temperature over time in the experiment (ANOVA RM, P0.05). Therefore, the antioxidant capabilities of the sweetgum extract were not affected by the storage time or temperature treatments studied in this work. For the antimicrobial experiment, there …

Unravelling The Mysteries Of Cognitive Decline In Cancer Survivors Using Functional Near Infrared Spectroscopy, Dhivya Srinivasan

Bioengineering Theses - Archive

In recent years, there has been an increased incidence of cognitive impairment in cancer survivors as a side-effect of chemotherapy. This troubling deterioration in cognitive ability is commonly termed as 'Chemo brain' in which patients have problems with working memory, executive function, decision-making, concentration, visuospatial skills and attention which can pose problems to get on with day-to-day life. Breast Cancer is primarily adopted as the model for studying effects of 'chemobrain'. A better understanding of neural correlates & hemodynamic changes related to cognitive deficits would help clinicians to design targeted treatments and cognitive rehabilitation interventions. Aim: The primary goal of …

Design And Development Of Real-Time Single And Dual-Wavelength Dcs Systems To Study Skeletal Muscle Blood Perfusion And Oxygen Metabolism, Chandan Ganesh Bangalore Yogananda

Bioengineering Theses - Archive

The aim of this study was to utilize Diffused Correlation Spectroscopy (DCS) to design and develop real-time single and dual wavelength systems and assess the physiological impacts of exercises on blood perfusion. The study consisted of 4 parts. Part 1 was to Re-design and develop a real-time single wavelength DCS system, part 2 was to validate this system by a cross validation method with the help of a concurrent study alongside Doppler ultrasound. Part 3 was to design & develop a real-time dual wavelength DCS system and part 4 was to validate the dual wavelength DCS system. DCS is a …

Light Reflectance Spectroscopy For The Detection Of Positive Surgical Margin Of Prostate Cancer, Henry Thek Chan

Bioengineering Theses - Archive

During a radical prostatectomy in the operating room, there exists a certain degree of risks of a surgical positive margin of prostate cancer. Positive surgical margins are defined as cancer that has spread beyond the margin of the prostate and into the surrounding tissues. Surgeons will be able to utilized the concept of Light Reflectance Spectroscopy (LRS) in real-time during surgery to help detect these positive surgical margins and to provide the surgeon a quantitative decision to remove the excess surrounding tissues. This modality would essentially lower the risks of positive surgical margins and prevent the recurrence of cancer, effectively …

The Biomechanics Of Music Performance, Rachel F. Bellisle, Jessika Decker

Senior Honors Projects

When first learning to play a wind instrument, beginner musicians are taught how to hold their instrument and correctly position their body. They are taught how to sit, where to put their hands and fingers on the keys, and how to hold their arms. This initial lesson on posture and hand positioning is often short, as one quickly moves on to learn the embouchure and breathing techniques that allow sound to be produced. As a musician progresses in skill, positioning is emphasized more, and they learn that it can affect their risk of strain or injury and improve their sound …

Common Treatments And Procedures Used For Fractures Of The Distal Radius And Scaphoid: A Review, Basel A. Khader, Mark R. Towler

Chemical and Biochemical Engineering Faculty Research & Creative Works

The distal radius and the scaphoid are the most commonly injured carpal bones among both active adults and the osteoporotic elderly. The purpose of surgical treatment is to restore form and function to the wrist. Depending on the nature of the fracture, either topical procedures or invasive surgery can be applied. This article critiques the treatments currently used for fixation of wrist fractures in order to drive the development of new materials to improve patient outcomes.

In Vivo Multi-Parametric Imaging Of Metastatic And Non-Metastatic Breast Cancer, Raisa B. Rasul

Biomedical Engineering Undergraduate Honors Theses

A current issue in cancer therapy is the characterization of metastatic tumors, which can increase ease of treatment and patient trials. We present an in vivo study of metastatic (4T1) and non-metastatic (4T1-TWIST KO) breast tumor sister cell lines to understand their metabolic behavior, determine differences in two modes of imaging (reflection & transmission), and observe effect of breathing higher oxygen percentage on vascular hemoglobin oxygen saturation. After injection of 10,000 cells into mice dorsal window chambers, the glucose intake and hemoglobin oxygen saturation was measured using a fluorescent glucose analog (2-NBDG) and hyperspectral trans-illumination imaging from 520-620 nm at …

Metabolic Response To Stress Differentiates Heterogeneous Cancer Cells With Varying Metastatic Potential, Kinan Alhallak

Biomedical Engineering Undergraduate Honors Theses

Intratumoral heterogeneity is ubiquitously present within primary tumors and contributes to intractable behaviors such as metastasis and mutability spatiotemporally. Mounting evidence has shown that heterogeneous cell populations can adversely affect cell metabolism and metastatic potential. The cell’s only fluorescent molecules within the electron transport chain, flavin adenine dinucleotide (FAD) and nicotinamide adenine dinucleotide (NADH), can allow the quantitation of cell metabolism. We demonstrate the use of the optical redox ratio (FAD/(NADH+FAD)) to determine the metabolic behaviors of a heterogeneous panel of cells with varying metastatic programs at normal conditions and following acute hypoxia. At normal conditions, we reveal an attenuation …

Role Of Trpv4 In Astrocyte Extracellular Matrix Production, Abby Terlouw

Biomedical Engineering Undergraduate Honors Theses

Traumatic Brain Injury (TBI) is an alteration of brain pathology following damage of the central nervous system (CNS) by an external force. In the CNS, glial scar formation often occurs following TBI, and astrocytes are widely believed to contribute to this scar formation. While the role of astrocytes in extracellular matrix (ECM) production is known, the exact mechanism(s) for this event remain unclear. One possible method is the activation of transient receptor potential vanilloid 4 (TRPV4). TRPV4 is a channel protein found in the astrocyte membrane which has been shown to generate intracellular calcium ions following mechanical stimulation. Previous research …

Characterization Of Murine Breast Cancer Cell Lines For Anti-Cancer Vaccine, Haven N. Frazier

Biological Sciences Undergraduate Honors Theses

Breast cancer is the most commonly diagnosed cancer in women and the second leading cause of cancer death among women in the United States (1). While treatments involving radiation and chemotherapy currently exist, disease must be detected early in order for the treatments to be somewhat effective, and there is no effective treatment after metastasis occurs (2). Additionally, current therapies do not mitigate tumor immunosuppression. Decreasing the tumor-associated immunosuppressive conditions while activating antitumor immunity could prevent recurrence and metastasis, possibly leading to an effective treatment for cancer (3). Tumor cell vaccines could possibly address this issue and have become a …

Alternative Treatment Technologies For Low-Cost Industrial And Municipal Wastewater Management, Alan J. Hodges

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Roughly the same volume of water that rushes over the Niagara Falls is produced as wastewater in North America. This wastewater is treated through a variety of means to ensure that it can be safely returned to the natural ecosystem. This thesis examines two novel means for this treatment, one biological and one physical-chemical in nature, namely, Rotating Algae Biofilm Reactor treatment and expanded shale augmented coagulation-flocculation.

Rotating algae biofilm reactors (RABRs) support biofilm algae growth, and in turn, the algae take up harmful contaminants from the wastewater. This system was tested in wastewater from petroleum refining operations. The efficacy …

Algae-Based Biofilm Productivity And Treatment Of Dairy Wastewater: Effects Of Temperature And Organic Carbon Concentration, Zachary T. Fica

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Production of dairy and associated products is a source of millions of gallons of wastewater every year. Water used in cleaning feeding stalls as well as the liquid component of the animal waste are two of the major volumetric components of this wastewater. This water is nutrient rich, often limiting the viability as a land applied fertilizer. However, these same nutrients could be used as an inexpensive feedstock for the cultivation of algae, which can then be used to produce downstream products including animal feed and aquaculture.

As part of this study, algal biomass was cultivated on dairy wastewater from …

Production Of Phenol-Formaldehyde Adhesives From Catalytic Pyrolysis Oil, Angela M. Akude

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Phenol-formaldehyde adhesives conventionally synthesized from fossil fuel resources pose two major concerns: sustainability issues and environmental concerns. The sustainability issues arise from the inevitable depletion of fossil fuel resources while
the environmental concerns, primarily, stem from the emission of volatile organic compounds. Thus, opting for alternative raw materials from renewable resources reduces the dependence on fossil fuels as well as promote the production of environmentally-friendly products.

The successful commercialization of bio-adhesives requires that the substitution of phenol with bio-oil during resin synthesis should be above 40%. In this study, catalytic pyrolysis oil produced from pinyon-juniper biomass and red mud alumina …

Anaerobic Digestion Of Wastewater: Effects Of Inoculants And Nutrient Management On Biomethane Production And Treatment, Jason Peterson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Due to population expiation and the increased awareness of the impact on the environment by wastewater treatment, improved wastewater treatment systems are needed to treat municipal and agricultural wastewater. Treating wastewater with oxygen decreases carbon compounds at the expense of energy to move carbon and oxygen to be in contact with each other. Anaerobic digestion of wastewater can reduce the cost by utilizing microbes to treat high amounts of carbon in wastewater without the need for extensive oxygen requirement. With a proper balance of nutrients, microbes also produce methane, a renewable energy source.

It has been suggested that microalgae be …

Development And Evaluation Of A Biocompatible Electroactive Sensor For Continuous Blood Pressure Measurement., Scott D. Cambron

Electronic Theses and Dissertations

Piezo-active composites have been implemented for sensing and transduction for decades. The 0-3 ceramic/polymer composite is one of the most common composite types used for sensing applications, owing to their tailorable properties of the two-phase composition, consisting of a three-dimensionally connected polymer/rubber matrix (inactive phase) with a dispersion of isolated piezo-ceramic particles (active phase). This thesis describes a method to develop novel biocompatible perivascular band comprised of a two-phase piezo-active composite to be fabricated using simple manufacturing processes. Biomaterials such as tissue scaffolds comprised of silk fibroin (SF) and chitosan (CS), and biocompatible soft rubbers will be implemented as the …

Pegda Pouches To Prevent Adhesions With In Vivo Bioreactor- Based Vascular Graft Strategies, Kranthi Vuppuluri

Theses and Dissertations

Different strategies have been investigated for the fabrication of vascular grafts. Using the peritoneal cavity of the patient as an “in vivo bioreactor” to recruit autologous cells to the implanted vascular conduit is one of the promising options. One of the main drawbacks with this strategy is the potential to form adhesions in the peritoneal cavity. In this project we are trying to address this potential side-effect by using polyethylene (glycol) diacrylate (PEGDA) to produce a hydrogel pouch into which the electrospun conduits are placed, with the goal of reducing the potential to form peritoneal adhesion after implantation. PEG is …

Development Of A Directional Bone Reaming System., Richard J Ackermann

Electronic Theses and Dissertations

Preparation of long bones such as the femur or tibia for placement of intramedullary devices for the treatment of fractures usually involves reaming with a series of central cutters driven by a drill-like device with a flexible shaft over a guide wire. The reamers sequentially enlarge the intramedullary canal into a tunnel of circular cross-section and a diameter appropriate for the procedure. The current technology is concentric, meaning that the system is self-centering within the original intramedullary canal and the expansion is symmetric with respect to the original centerline. A novel system for laterally deflecting the head of a 12mm …

Monitoring Heart Rate Variability In Relation To Sleep Quality, Yi-Tzu Wang

Theses and Dissertations

The development and analysis of heart rate variability for sleep quality monitoring have been concerned for many years in human being. Sleep is a physiological process that is essentially maintaining the function of human body. Heart rate variability have undergone much-needed development to assess the effects such as sleep disorder, insomnia, high blood pressure, stress and even diabetes. Accurately evaluating physiological signals that are practical to measure the sleep quality in individuals monitoring heart rate variability is an important activation of diverse biological systems during sleep. A noteworthy role of measuring the heart rate variability is played by the autonomic …

Synthesis And Characterization Of 3-D Tissue Engineered Tumor Models For Melanoma Study And Extracellular Matrix Investigation, Joseph L. Spano

Theses and Dissertations

Introduction: Melanoma is the deadliest form of skin cancer, projected to claim over 9,700 American lives and necessitate $3.3 billion in care and treatment costs in 2017. In order to reduce the socioeconomic burdens and lessen cancer-related mortality and morbidity, advancements in early-stage diagnostics and treatments are essential. Two-dimensional (2-D) tissue culture treated plastics (TCTP) are reductionist in vitro models that are commonly used in drug development and preclinical drug evaluation. These models poorly recapture the native tumor microenvironment (TME), providing unrealistic growth conditions, and are thus poorly suited to measure the efficacy of newly emerging therapies. We have developed …

Sensitivity Of Diffuse Reflectance Spectroscopy To Dose- And Depth-Dependent Changes In Tumor Oxygenation After Radiation Therapy, Daria Semeniak

Graduate Theses and Dissertations

Along with chemotherapy, immunotherapy, and surgery, radiotherapy is one of the most common treatments used against cancer. Around 50% of all cancer patients undergo radiation therapy. While for some patients radiotherapy works efficiently and lead to a complete cancer disappearance, for others treatment outcome may be less favorable due to radioresistance processes happening within a tumor on the molecular level. Radioresistance remains a big challenge for modern oncology. The ability to identify radioresistance at the early stage of radiotherapy would help physicians to improve therapy efficiency. At the current moment, despite the rapid progress in cancer understanding and diagnostic modalities, …

Assessment Of Kinematics And Electromyography Following Arthroscopic Single-Tendon Rotator Cuff Repair, Jessica M. Fritz, Ryan R. Inawat, Brooke A. Slavens, John R. Mcguire, Dean W. Ziegler, Sergey S. Tarima, Steven I. Grindel, Gerald F. Harris

Biomedical Engineering Faculty Research and Publications

Background

The increasing demand for rotator cuff (RC) repair patients to return to work as soon as they are physically able has led to exploration of when this is feasible. Current guidelines from our orthopedic surgery clinic recommend a return to work at 9 weeks postoperation. To more fully define capacity to return to work, the current study was conducted using a unique series of quantitative tools. To date, no study has combined 3-dimensional (3D) motion analysis with electromyography (EMG) assessment during activities of daily living (ADLs), including desk tasks, and commonly prescribed rehabilitation exercise.

Objective

To apply a quantitative, …

Segmental Kinematic Analysis Of Planovalgus Feet During Walking In Children With Cerebral Palsy, Karen M. Kruger, Katherine A. Konop, Joseph Krzak, Adam Graf, Haluk Altiok, Peter A. Smith, Gerald F. Harris

Biomedical Engineering Faculty Research and Publications

Pes planovalgus (flatfoot) is a common deformity among children with cerebral palsy. The Milwaukee Foot Model (MFM), a multi-segmental kinematic foot model, which uses radiography to align the underlying bony anatomy with reflective surface markers, was used to evaluate 20 pediatric participants (30 feet) with planovalgus secondary to cerebral palsy prior to surgery. Three-dimensional kinematics of the tibia, hindfoot, forefoot, and hallux segments are reported and compared to an age-matched control set of typically-developing children. Most results were consistent with known characteristics of the deformity and showed decreased plantar flexion of the forefoot relative to hindfoot, increased forefoot abduction, and …

The Agile Approach In Pharmaceutical Software Development, Priyal Bhargava

Honors Scholar Theses

The Agile development model is increasingly being adopted by the pharmaceutical industry for software development. Currently, there are several software projects under development by pharmaceutical companies that are using the Agile process. Other methodologies such as the Waterfall process, a traditional approach to project management, have evolved over time and been compared against the Agile method. Also there are conflicts of interest which should be taken into consideration when in a pharmaceutical setting such as abiding by FDA regulations. In addition, Agile’s sub-divisional approaches add to the versatility of how this iterative approach is applied in the pharmaceutical industry. The …

Patient-Derived Hydrogel As A Sacrificial Matrix For Efficient Cell Loading, Shalmli U. Joshi

Honors Scholar Theses

The field of tissue engineering focuses on delivering patient-derived stem cells to the body through the use of degradable biomaterials, such as hydrogels, which are infused into engineered scaffolds. Hydrogels act as templates to support and guide cells towards the regeneration of new tissue. In this study, we introduce a completely intraoperative procedure for obtaining a fibrin hydrogel for tissue engineering applications. In the past, fibrin hydrogel has been commonly formed by combining fibrinogen protein with animal-derived thrombin. Instead, we have developed an automated, and therefore reproducible, protocol to isolate and form fibrin hydrogel without the use of animal-derived thrombin. …