Biomedical Engineering and Bioengineering Commons^™

Application Of Animal-Free Recombinant Bioactive Protein Supplements To Improve The Performance Of Cell-Based Viral Vaccine Production, Kenneth Bertram, Marina Ross, Domenica Cavallaro, Larissa Chirkova, Geoffrey Francis

Vaccine Technology III

Animal cell culture for the production of viral vaccines has been performed for more than 50 years, and currently this technology is expanding rapidly to meet present and future demands of the health sector. The development and regulatory approval of continuous cell lines for manufacturing viral vaccines has brought numerous benefits to production processes. However, greater advances in the last decade have been achieved in mammalian cell production of biological therapeutics, including monoclonal antibodies, hormones, growth factors, cytokines and clotting factors. We and others have contributed to these upstream advances by improving cell culture media with the development of animal-free …

Development Of Recombinant Protein Based Chemical Conjugate Malaria Vaccines Targeting The Pre-Erythrocytic Stage, Transmission Blocking, Or Both, David L. Narum, Nicholas Macdonald, David Jones, Ruth Ellis, Yimin Wu, Patrick E. Duffy

Vaccine Technology III

The development of a Plasmodium falciparum malaria vaccine is critical for future control and elimination programs. Recombinant protein based chemical conjugate vaccines, covering different parasite stages, are being developed due to complexity of the parasite and sub-optimal immunogenicity of recombinant malaria proteins in humans, respectively. Chemical conjugation of recombinant malaria proteins to carrier proteins improves their immunogenicity in animal studies. A transmission blocking vaccine comprised of the ookinete protein Pfs25 chemically conjugated to Pseudomonas aeruginosa ExoProtein A (EPA) is currently being developed for pilot scale cGMP production. Bulk lots of Pfs25 and EPA have already been produced and released following …

Clinical Development Of Formulated Therapeutic And Prophylactic Dna-Based Vaccines, Alain Rolland

Vaccine Technology III

Over the recent years, plasmid DNA vaccines have reached licensure against infectious hematopoietic necrosis virus in farmed salmon (Canada), West Nile virus in horses and metastatic melanoma in dogs (United States). A number of approaches are currently evaluated in clinical trials to enhance the potency of DNA vaccines in humans, including formulation of DNA with delivery systems and adjuvants as well as administration of DNA with devices. This presentation will report on the development of TransVaxTM, a poloxamer-formulated therapeutic DNA vaccine against human cytomegalovirus (CMV) in transplant patients. A vaccine that increases CMV-specific T-cell responses could reduce CMV reactivation after …

Establishing A Platform For Spray Drying Inhalable Vaccines In South Africa, Willem Germishuizen, L Venter, A Khosa, F Mudau, P.B. Fourie, B Pulliam, M Kabadi, A Schiermeier, D.A . Edwards

Vaccine Technology III

Mycobacterium bovis BCG is the current vaccine for tuberculosis (TB). However, BCG as it is currently administered shows highly variable efficacy in protecting adults against TB. The natural route of infection of TB is via inhalation of bacilli-containing aerosols and it is postulated that immunization by the natural route of infection may lead to a greater immunity given the fact that the lungs are the primary target of infection. By eliciting both local and systemic immune responses, it is anticipated that an inhaled form of BCG will offer greater protection against pulmonary TB.

Current commercial BCG vaccine preparations are filled …

The Gel Documentation System: A Cornerstone To The Implementation Of The Introduction To Biotechnology And Introduction To Bioinformatics Cross-Disciplinary Course Series, Marcy Kelly, Gregory Lampard, Constance Knapp

Cornerstone 3 Reports : Interdisciplinary Informatics

No abstract provided.

Dielectrophoretic Separation Of Mouse Melanoma Clones, Ahmet C. Sabuncu, Jie A. Liu, Stephen J. Beebe, Ali Beskok

Bioelectrics Publications

Dielectrophoresis (DEP) is employed to differentiate clones of mouse melanoma B16F10 cells. Five clones were tested on microelectrodes. At a specific excitation frequency, clone 1 showed a different DEP response than the other four. Growth rate, melanin content, recovery from cryopreservation, and in vitro invasive studies were performed. Clone 1 is shown to have significantly different melanin content and recovery rate from cryopreservation. This paper reports the ability of DEP to differentiate between two malignant cells of the same origin. Different DEP responses of the two clones could be linked to their melanin content.

Epidermal Neural Crest Stem Cell (Epi-Ncsc)--Mediated Recovery Of Sensory Function In A Mouse Model Of Spinal Cord Injury, Yao Fei Hu, Krishnaj Gourab, Clive Wells, Oliver Clives, Brian D. Schmit, Maya Sieber-Blum

Biomedical Engineering Faculty Research and Publications

Here we show that epidermal neural crest stem cell (EPI-NCSC) transplants in the contused spinal cord caused a 24% improvement in sensory connectivity and a substantial recovery of touch perception. Furthermore we present a novel method for the ex vivo expansion of EPI-NCSC into millions of stem cells that takes advantage of the migratory ability of neural crest stem cells and is based on a new culture medium and the use of microcarriers. Functional improvement was shown by two independent methods, spinal somatosensory evoked potentials (SpSEP) and the Semmes-Weinstein touch test. Subsets of transplanted cells differentiated into myelinating oligodendrocytes. Unilateral …

Development Towards A Protocol To Test For The Creation Of A “Diabetic” Environment In A Blood Vessel Mimic, Kaitlyn Kirk, Development Towards A Protocol To Test For The Creation Of A “Diabetic” Environment In A Blood Vessel Mimic

Biomedical Engineering

Diabetes mellitus affects a huge proportion of people; about 6% of the population in the US had diabetes in 2007 (16). Diabetes promotes dyslipidemia, an imbalance in the circulating levels of lipids and lipoproteins, and diabetes is one of the leading risk factors for coronary artery and peripheral vascular diseases (11, 51, 52).  There are two types of diabetes; type 1 diabetes accounts for about 10% of diagnosed cases and type 2 accounts for about 90% (11).  Diabetic patients react differently to stents and there is a great need to enhance the treatment.  The goal of this project was, and …

Frontal Plane Humeral Elevation Effects On The Pullout Strength Of Suture Anchors Used In Rotator Cuff Repair, Peter Joshua Larson

Master's Theses

The rotator cuff offers the stabilizing forces necessary for fine arm movement. The most common injury to the rotator cuff is the partial or full thickness tear of the supraspinatus tendon, which attaches the humerus to the scapula on the superior side, due to an acute or chronic injury. These injuries account for more than 4.5 million physician visits per year. Depending on the degree of tear, arthroscopic surgery may be needed to reattach the torn tendon to the humeral head. The current preferred method of surgery involves the insertion of a suture anchor into the humeral head that ties …

Influence Of Morphology And Processing On Xps Characterisation Of Sro-Ca-Zno-Sio2 Glass, F. R. Laffir, A. W. Wren, Mark R. Towler

Chemical and Biochemical Engineering Faculty Research & Creative Works

A study was conducted to demonstrate the influence of morphology and processing on X-ray photoelectron spectroscopic (XPS) characterization SrO-Ca-ZnO-SiO₂ glass. The glass formulation being investigated was originally synthesized for developing a novel glass polyalkenoate cement (GPC). High resolution photoelectron spectra of Zn 2p, Ca 2p, Sr 3d, Si 2p, O 1s, and C 1s were recorded for the different forms of glass. It was observed that there was preferential enrichment of Zn²⁺ with an increase in the proportion of NBO for the investigated BT101 glass. The relative atomic concentration of Zn in BT101 glass was high and resulted …

Antenna Development For Radio Frequency Hyperthermia Applications, Sergio Curto

Doctoral

This thesis deals with the design steps, development and validation of an applicator for radio frequency hyperthermia cancer therapy. An applicator design to enhance targeted energy coupling is a key enabler for preferential temperature increments in tumour regions. A single-element, near-field approach requires a miniaturised solution, that addresses ergonomic needs and is tolerant to patient anatomy. The antenna war-field rriodality and the high-dielectric patient loading introduce significant analytical and computational resource challenges. The antenna input impedance has to be sufficiently insensitive to in-band resonant cletuning and the fields in the tissue can he targeted to selected areas in the patient. …

Cell-Mediated Elastic Matrix Regeneration Toward Regression Of Abdominal Aortic Aneurysms, Carmen Gacchina

All Dissertations

Abdominal aortic aneurysms (AAAs) are typically fusiform (symmetric) dilations of the aortic wall most commonly arising below the renal arteries. The progression is typically associated with an activated smooth muscle cell (SMC) phenotype, diminished density of mature medial elastic fibers, and an elevated presence of matrix-degrading enzymes (e.g., matrix-metalloproteases; MMPs), which may ultimately lead to vessel rupture. Currently, no surgical or non-surgical methods are available to regress AAAs via regeneration of new elastin matrices to regain normal vessel contour, particularly due to the inherently poor elastin synthesis by adult vascular cells and absence of methods to stimulate the same. Previously, …

Effect Of Simulated Visual Impairment On Nighttime Driving Performance, Joanne Wood, Alex Chaparro, Trent Carberry, Byoung Sun Chu

Publications

Purpose. This study investigated the effects of simulated visual impairment on nighttime driving performance and pedestrian recognition under real-road conditions.

Methods. Closed road nighttime driving performance was measured for 20 young visually normal participants (M = 27.5 +- 6.1 years) under three visual conditions: normal vision, simulated cataracts, and refractive blur that were incorporated in modified goggles. The visual acuity levels for the cataract and blur conditions were matched for each participant. Driving measures included sign recognition, avoidance of low contrast road hazards, time to complete the course, and lane keeping. Pedestrian recognition was measured for pedestrians wearing either black …

Effects Of Monoclonal Anti-Abeta Antibodies On The Amyloid Beta Peptide Fibrillogenesis And Their Involvement In The Clearance Of Alzheimer's Disease Plaques, Jeffy Pilar Jimenez

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease (AD) is the most common cause of senile dementia worldwide. AD is a neurodegenerative disorder characterized by the loss of memory and language skill, collapse of the cognitive function, and distortion of social behavior. As of today, the onset mechanisms of AD and cure are unknown; however, three hallmarks are commonly encountered: extra and intracellular accumulation of amyloid beta (A!) peptide plaques, formation of intracellular neurofibrillary tangles, and inevitable neuronal death. Hypothetically, a possible scenario provoking or involved in the onset of AD is a cascade effect that starts with an imbalance in the production and clearance of …

Exploiting The Concept Of Hysteresis In Ankle Dysfunction And Ankle Strengthening, Akshay Narendra Pujara

Theses

The ankle joint is an important part of the body which is responsible for normal gait cycle. Dorsiflexion, plantar flexion, inversion and eversion are four types of movements controlled by the ankle joint. All these movements are controlled by the ligaments and the muscles associated with the ankle. Disorders of these ligaments and muscles leads to ankle dysfunction.

The hysteresis concept was employed to determine if there is any ankle dysfunction. A recently developed device, the Ankle Torsion Monitor (AnTm) was employed to evaluate the viscoelasticity and stiffness of the ankle joint. The greater the hysteresis loop area (HLA) the …

Automation Of Anatomic Torsion Monitor For Evaluation And Improvement Of Low Back Dysfunction, Vishal Kumar Singh

Theses

The existing Anatomical Torsion Monitor (ATM) to evaluate mechanical stiffness and viscoelasticity of the low back suffers from various inherent defects. This has to be replaced by an improved device. Also the existing ATM cannot provide oscillations to the low back.

The main objective is to automate the existing ATM for evaluating the low back immediately using objective methods. The specific objective is to provide oscillations for improving the low back dysfunction.

The laser platform and the target chart for recording the readings are dispensed with the existing ATM. Instead, the ultrasound transducers are attached to the pads to record …

Comparison Between Different Techniques Of Preprocessing For Resting State Fmri Analysis, Megha Girdhar

Theses

Resting state functional connectivity as the name suggests is defined as significant temporal correlation between spatially distinct regions of the brain during rest. In this thesis, fMRI resting state dataset was analyzed using different available processing techniques with the same fMRI data to study differences between the various methods. All the imaging data from each of the subjects was processed in an identical fashion. The same method was used for detecting connectivity. The number of independent components in the data was used as the base to differentiate the effect of each of these methods. Independent component analysis was performed on …

Axon Guided Axon Growth In Drg Neurons From The Spinal Cord Of Rat Embryos, Neha Jain

Theses

Early studies in the area of neuronal physiology and injury have shown that axons are guided by a number of physical as well as chemical cues. Although the guidance effect of initial axons and their processes on later axons has been shown from a development point of view, little light has been thrown on the subject from the injury point of view. This important ability of axons which plays a key role during development and formation of neuronal networks could become crucial in repairing damaged nerves and disrupted neuronal tracts.

In the following study, the guidance effect of an older …

The Design And Validation Of A Novel Computational Simulation Of The Leg For The Investigation Of Injury, Disease, And Surgical Treatment, Joseph Iaquinto

Theses and Dissertations

Computational modeling of joints and their function, a developing field, is becoming a significant health and wellness tool of our modern age. Due to familiarity of prior research focused on the lower extremity, a foot and ankle 3D computational model was created to explore the potential for these computational methods. The method of isolating CT scanned tissue and rendering a patient specific anatomy in the digital domain was accomplished by the use of MIMICS™ , SolidWorks™, and COSMOSMotion™ – all available in the commercial domain. The kinematics of the joints are driven solely by anatomically modeled soft tissue applied to …

Effects Of Static Stretching On Foot Velocity During The Instep Soccer Kick, Craig D. Workman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to assess the acute effects of static stretching on foot velocity at impact with a soccer ball. Eighteen Division I female soccer athletes underwent two test conditions separated by 48 hr. Each condition was randomly assigned and began by placing four retro-reflective markers on bony landmarks of the ankle (total of eight markers, four on each ankle). One condition was the no-stretch condition, in which each participant performed a self-paced jog for 5 min as a warm-up, and then sat quietly for 6 min before performing three maximal instep kicks into a net. The …

Waste To Work Using Eccrine Sweat Battery Modules To Enhance Pedestrian Safety, Lauren J. Vesty

Renée Crown University Honors Thesis Projects - All

There is a need for increased pedestrian visibility. Improved athletic clothing with built in visibility features would enhance a roadside athlete’s safety. This experiment aims to create such an athletic shirt, while incorporating wearable biotechnology. A sweat battery that produced a voltage of 0.49 V and a current of 5.16 mA was created to power the visibility aspects of the shirt. The battery uses an aluminum anode, a carbon cathode, a sponge block as the container and semi-permeable membrane, and the body’s eccrine sweat as the electrolyte solution. The battery cells were arranged into blocks to produce an optimal current …

Electrospun Scaffolds For Directed Pancreatic Differentiation Of Human Ips Cells, Karl Sonntag

Biological and Agricultural Engineering Undergraduate Honors Theses

In this work, an electrospinning device for fabricating three-dimensional scaffolds was constructed. The device can be used to produce tissue engineered scaffolds electrospun for directing the differentiation of human induced pluripotent stem (iPS) cells into glucose-responsive, insulin-secreting cells for diabetes treatment. Electrospinning utilizes high electrical charge to generate nanofibers from polymers dissolved in solution. A standard setup for such a device includes a high-voltage power source, a syringe pump, and a grounded collector. The process works by dissolving the polymer in a volatile solvent, and filling a syringe with the polymer/solvent mixture. Next, the syringe is locked into place in …

Effects Of Reamer-Femoral Component Offset On Cement Mantle Penetration In Hip Resurfacing Arthroplasty, Mark Lloyd Paulick

Master's Theses

Hip resurfacing arthroplasty has changed the treatment of end stage arthritis without severe deformity for young, active adults. Presently, there are varying clinical approaches to implant design selection and cementation techniques. The purpose of this project is to determine what amount of reamer-femoral component offset allows for the best cement penetration into the femoral head.

Rapid prototyped femoral component models were produced with reamer femoral component offsets of 0.0 mm, 0.5 mm, and 1.0 mm. After implantation onto models of reamed femoral heads made from high-density open-cell reticulated carbon foam, cement penetration was assessed from cross-sections of the foam-implant unit. …

Liquid Crystalline Elastomers: Effects Of Substrate Anisotropy On Cell Behavior, Christine Depompeo

Renée Crown University Honors Thesis Projects - All

One of the major challenges in tissue engineering today is inducing organization at the cellular level in vitro. Most biological tissues exhibit anisotropic behavior. They perform differently and have different mechanical properties in different directions. This environment is difficult to mimic with traditional cell culturing methods. The development of anisotropic cell substrates may have the potential to encourage the organization required at the cellular level to induce in vitro tissue formation, an exciting prospect for the advancement of the field of tissue engineering.

It is well known that liquid crystalline elastomers (LCEs) are highly organized polymers with temperature-dependent properties. …

Using The Continuous Wavelet Transform To Characterize Differences Between Impact Signals From Non-Cleated And Cleated Turf Shoes, Wayne Robert Fischer

Boise State University Theses and Dissertations

The continuous wavelet transform was used to characterize the time-frequency differences between impact forces from non-cleated and cleated turf shoes among male football athletes who perform cut and run activities. This research is significant because it elucidates how athletes experience different impact force and torque frequency content based on the type of shoe they are wearing. The complex Morlet mother wavelet was used to analyze all ground reaction force and vertical ground reaction moment signals to create time-frequency power spectrum plots. For each signal, a statistical confidence interval was calculated and displayed along with the cone of influence caused by …

Finite Element Modeling Of Icd Lead Silicone Soft-Tips, Jose J. Lepe

Master's Theses

Although highly underutilized by the medical device industry, Finite Element Analysis (FEA) in the development of new technologies is gaining popularity as regulatory bodies such as the Food and Drug Administration (FDA) begin to require additional proof of safety through scientific methods. Non-linear FEA allows engineers to realistically simulate the mechanical behavior of implants as seen in the in-vitro, or in some cases, the in-vivo configurations. The work presented in this report investigates how computational methods can be used to simulate the interaction of a St. Jude Medical silicone soft-tip as it passes through a Peel-Away Sheath (i.e. introducer). In …

Osteoclast-Mediated Cell Therapy As An Attempt To Treat Elastin-Specific Vascular Calcification, Chartrisa Simpson

All Dissertations

Vascular calcification occurs during physiological aging and is clinically recognized as a major risk factor for myocardial infarction, systolic hypertension, heart failure and coronary insufficiency caused by loss of aortic recoil. Medial arterial calcification is an active remodeling process that involves the deposition of bone-specific matrix in close association with elastic fibers. Elastin, a major structural protein in the extracellular matrix of arterial walls, provides elastic recoil to the arteries. Increasing evidence suggests that vascular calcification share features with skeletal bone formation such as bone matrix deposition and bone resorption. Bone morphogenetic proteins and bone cells have been found in …

A Synthetic Biological Engineering Approach To Secretion- Based Recovery Of Polyhydroxyalkanoates And Other Cellular Products, Elisabeth Linton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The costs associated with cellular product recovery commonly account for as much as 80% of the total production expense. As a specific example, significant recovery costs limit commercial use of polyhydroxyalkanoates (PHA), which comprise a class of microbially-accumulated polyesters. PHAs are biodegradable compounds that are of interest as a sustainable alternative to petrochemically-derived plastics. Secretion-based recovery of PHAs was studied to decrease PHA production costs. Type I and II secretory pathways are commonly used for the translocation of recombinant proteins out of the cytoplasm of E. coli. Proteins were targeted for translocation using four signal peptides (HlyA, TorA, GeneIII, …

The Hypericum Perforatum Herb As An Antimycobacterial Agent And Its Implications As An Additional Tuberculosis Medication, Trent W. Mortensen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An immediate demand exists for new tuberculosis (TB) antibiotics due to the ever-increasing spread of drug-resistant strains. The drug-development process goes through four phases, the first (Phase 0) of which is to demonstrate and investigate drug effectiveness and toxicity. This research investigated the effectiveness of the Hypericum perforatum herb (commonly St. John's wort (SJW)) in its growth inhibition of mycobacteria and its viability effect on human lung cells.

Organic-solvent SJW extracts were effective at inhibiting every nonpathogenic genetically sequenced Mycobacterium isolate currently available (six isolates) in preliminary studies. Quantitative studies of five Mycobacterium isolates showed an order of concentration sensitivity …

Comparative Studies On Oxygen Mass Transfer For The Design And Development Of A Single-Use Fermentor, Kristan L. Sorenson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Accurate experimental oxygen mass transfer coefficient, a measure of how quickly oxygen travels from a gas bubble to the bulk liquid, is important for comparing performance and for evaluating the oxygen transfer capability of a fermentor. Delays in probe response and changing gas volumes upon start-up of gassing affect the accuracy of oxygen transfer measurements. To mitigate these inaccuracies, a standard correction procedure for oxygen mass transfer data was established for highly oxygenated, well-mixed fermentation systems. Probe response time correction was generated by applying a second-order response model to dissolved oxygen probes and shown to be effective within 4%. By …