Engineering Commons^™

Automated Foam Control In Single-Use Bioreactors Using The Single Use Foam Probe, Jordan Cobia, Ben Madsen, Emilee Madsen, Alex Hodge

Single-Use Technologies III: Scientific and Technological Advancements

Headspace foam in bioreactors can cause significant issues to a biological process among which are unfavorable metabolic conditions for cells, increased shear from bursting bubbles, cell death/entrainment, mass transfer interference and potential fouling of exhaust filters which can allow a point of entry for contamination or lead to pressure build-up and possible failure of the bioprocess container. This study investigates the efficacy of using a single use foam probe coupled with an automated response of an antifoam addition with an integrated DeltaV controller. Two 50L S.U.B.s run in parallel clearly illustrate differences; the first employing foam control using the foam …

Polyestyrene And Low Density Polyethylene Oregano´S Essential Oil Functionalization For Possible Antimicrobial Active Packaging Applications, Edgar Díaz-Acosta, Claudia Rodríguez-González, Laura Valencia-Gómez, Santos Adriana Martel-Estrada, Maria Hernández-González, Hortensia Reyes-Blas, Imelda Olivas Armendáriz 1761524

Journal of Applied Packaging Research

Low density polyethylene (LDPE) and polystyrene (PS) samples were functionalized with oregano´s essential oil (OEO) for possible antimicrobial packing applications. Different concentrations of OEO were used (0.5-1.5 % v/v). Polymers were UV light irradiated during 60 min to improve the OEO adhesion through the polar groups (COOH, OH or COO-) generation on their surface. The effect of OEO different concentration on their antimicrobial activity, mechanical properties and cytotoxicity of the functionalized polymers were analyzed. All functionalized samples showed antimicrobial activity against Gram positive and Gram negative bacteria and this activity increased with OEO concentration. The addition of OEO did not …

Experimental Tests And Numerical Simulations For Failure Investigation On Corrugated Boxes Used On Household Appliance Packaging, Diego Fernandes Rodrigues, José Carlos Pereira

Journal of Applied Packaging Research

Packages made of corrugated paper are fundamental to the protection, transportation and handling of the appliance product market. During the storage and sales stages of a product, the package must resist compressive loads in different directions beyond moderate impacts. In this context, the objective of this work is to develop and implement a post-processor that allows the simultaneous analysis of two of the most common failure modes of packages made of corrugated paper: failure due to tensile or compressive stress limit, and failure due to local buckling, when the buckling of the faces of the corrugated paper between two peaks …

Ph Evolution In Solution After Contact With Multilayer Films After Different G- Irradiation Doses And Thus Reconciliation Of Ph And Toc With Carboxylic Acids Detected By Ion Chromatography, Samuel Dorey, Fanny Gaston, Nathalie Dupuy, Magali Barbaroux, Sylvain R.A. Marque

Single-Use Technologies III: Scientific and Technological Advancements

For a number of various uses (storage, mixing, freezing, transportation, formulation, and filling) biopharmaceutical solutions are stored in sterile single-use plastic bags. Material transfers can then occur between containers and contents. These migrations, of different types, depend on the physicochemical characteristics of the material (composition, pH, solubility, viscosity, molecular weight, etc.), the nature of the product (solid, semi-solid and liquid) and the conditions of the material utilization. In the case of single-use polymers, γ-irradiation sterilization of the polymer is often carried out. The interactions could be therefore influenced by the dose and the contact time between the container and the …

Intensive Mentoring And Micro-Electronics Research For Students In Engineering (Immerse) 2016 Mentoring Environment Grant, Aaron Hawkins

Journal of Undergraduate Research

The MEG funds used for this projects supported undergraduate students working in the IMMERSE program starting in the Summer of 2016. IMMERSE employed a total of 49 students. Portions of the hourly wages charged by these students for their research work came from this MEG grant.

Using Electronic Health Records To Characterize Prescription Patterns: Focus On Antidepressants In Nonpsychiatric Outpatient Settings, Joseph J. Deferio, Tomer T. Levin, Judith Cukor, Samprit Banerjee, Rozan Abdulrahman, Amit Sheth, Neel Mehta, Jyotishman Pathak

Publications

Objective To characterize nonpsychiatric prescription patterns of antidepressants according to drug labels and evidence assessments (on-label, evidence-based, and off-label) using structured outpatient electronic health record (EHR) data.

Methods A retrospective analysis was conducted using deidentified EHR data from an outpatient practice at a New York City-based academic medical center. Structured “medication–diagnosis” pairs for antidepressants from 35 325 patients between January 2010 and December 2015 were compared to the latest drug product labels and evidence assessments.

Results Of 140 929 antidepressant prescriptions prescribed by primary care providers (PCPs) and nonpsychiatry specialists, 69% were characterized as “on-label/evidence-based uses.” Depression diagnoses were associated …

Managing Diffuse Phosphorus At The Source Versus At The Sink, Katrina A. Macintosh, Brooke K. Mayer, Richard W. Mcdowell, Stephen M. Powers, Lawrence A. Baker, Treavor H. Boyer, Bruce E. Rittmann

Civil and Environmental Engineering Faculty Research and Publications

Judicious phosphorus (P) management is a global grand challenge and critical to achieving and maintaining water quality objectives while maintaining food production. The management of point sources has been successful in lowering P inputs to aquatic environments, but more difficult is reducing P discharges associated with diffuse sources, such as nonpoint runoff from agriculture and urban landscapes, as well as P accumulated in soils and sediments. Strategies for effective diffuse-P management are imperative. Many options are currently available, and the most cost-effective and practical choice depends on the local situation. This critical review describes how the metrics of P quantity …

Noncontact Speckle Contrast Diffuse Correlation Tomography Of Blood Flow Distributions In Tissues With Arbitrary Geometries, Siavash Mazdeyasna, Chong Huang, Mingjun Zhao, Nneamaka B. Agochukwu, Ahmed A. Bahrani, Lesley Wong, Guoqiang Yu

Biomedical Engineering Faculty Publications

A noncontact electron multiplying charge-coupled-device (EMCCD)-based speckle contrast diffuse correlation tomography (scDCT) technology has been recently developed in our laboratory, allowing for noninvasive three-dimensional measurement of tissue blood flow distributions. One major remaining constraint in the scDCT is the assumption of a semi-infinite tissue volume with a flat surface, which affects the image reconstruction accuracy for tissues with irregular geometries. An advanced photometric stereo technique (PST) was integrated into the scDCT system to obtain the surface geometry in real time for image reconstruction. Computer simulations demonstrated that a priori knowledge of tissue surface geometry is crucial for precisely reconstructing the …

Field Instrumentations And Numerical Analysis Of Geosynthetic Reinforced Soil – Integrated Bridge System (Grs-Ibs), Allam Ardah

LSU Doctoral Dissertations

Geosynthetic reinforced soil (GRS) is a special soil with geosynthetic fabric closely stacked in layers as soil stabilization and considered an alternative design method to the conventional bridge support technology. In this research study, a field case study of Maree Michel bridge, which is located in Route LA 91 Vermilion Parish in Louisiana, was instrumented with six different types of instrumentations to monitor the performance of GRS-IBS bridge abutment and to develop 2D and 3D finite element models. The instrumentations include Shape Acceleration Array (SAA), earth pressure cells, strain gauges, piezometers, and thermocouples. Additionally, surveying was conducted at the bridge …

The Proper Use Of Extractables Data - Aspects Beyond Extractables-Measurment, Samuel Dorey, Armin Hauk, Roberto Menzel, Thomas Loewe, Ina Pahl

Single-Use Technologies III: Scientific and Technological Advancements

Appropriate extraction techniques for SUS/SUT and methods for analysis of extractables have been intensively discussed over the last years. Today, several proposals for common methods are available and used to conduct extractables studies in the bio-pharmaceutical industry. Therefore it is expected that the number of available extractables data will significantly rise over the next years and it is worth to (re-)consider their proper use for materials and device qualification and risk assessment. While this exercise is straightforward for container closure systems (CCS), for SUS/SUT the situation is more complex. In CCS applications, a single drug product, in contact with a …

Tuflux Tpe Tubing For Pharma Processing, Pradnya Parulekar, Michael Drechsel

Single-Use Technologies III: Scientific and Technological Advancements

The guidelines for launching new products and manufacturing those approved products are becoming more stringent on a routine basis. Quality certifications, clean room requirements, forwards and backwards traceability on all components are the new standard for providing FDA-approve products. Part of insuring that quality criteria are being met is to understand how the raw material is created, how it is processed in to a technologically useful processing aid (tube) and then how is that tube tested. Thermoplastic resin is the key ingredient in creating TuFlux tubing. The broad chemistry footprint allows specialized compounders to create unique blends of components (using …

Mixing Operations For 50 L To 2000 L Single-Use Mixer: Liquid-Liquid Mixing Characterization And Slurry Suspension, Jordan Cobia, Kevin Mullen, Becky Staerk

Single-Use Technologies III: Scientific and Technological Advancements

Mixers are employed throughout all parts of bioprocessing. Single-use mixers (S.U.M.) are commonly used for upstream operations including media formulation and hydration, media holding, sterile filtration and downstream operations including media and product storage, viral inactivation, buffer preparation, and resin/slurry preparation for column packing. This study presents the automation of both simple and complex mixing procedures using the Thermo Fisher Touchscreen Control Panel. Also investigated are the selection method for scalable mixing parameters allowing normalized mixing performance across all single use mixer sizes. The following applications are demonstrated in this study:

• Characterization of scalable mixing parameters by comparing power input …

Conference Program, Weibing Ding, Martina Micheletti, Robert Repetto

Single-Use Technologies III: Scientific and Technological Advancements

No abstract provided.

Full Proceedings, 2018, Jianshun Zhang

International Building Physics Conference 2018

Full conference proceedings for the 2018 International Building Physics Association Conference hosted at Syracuse University.

Qualification Of Low Drift Single-Use Ph Sensors For Use In Single-Use Bioreactor Platforms, Jason Brown, Alexander J. Hodge, Nephi Jones

Single-Use Technologies III: Scientific and Technological Advancements

The biopharmaceutical industry is currently limited by access to low drift, gamma stable, easy-to-integrate single-use pH sensors for application in single-use bioreactors (S.U.B.). Hamilton Company has developed the OneFerm VP 70 sensor, a unique single-use glass electrode, which provides an additional option for customers wishing to implement reliable single-use pH sensing capabilities in the S.U.B. platform. In order to demonstrate the efficacy of these devices, a series of qualification experiments were carried out using a Thermo Fisher Scientific 50L bioprocess container (BPC) custom-fitted with Hamilton OneFerm sensors. BPCs were manufactured in a cGMP facility and allowed to age for a …

An Example Of Thermoset – Silicone, Csilla Kollar

Single-Use Technologies III: Scientific and Technological Advancements

Abstract is not available.

Single-Use Ph Sensor Via A Coplanar Ph Glass Electrode Design, Cd Feng, Robert Garrahy

Single-Use Technologies III: Scientific and Technological Advancements

The elimination of high temperature and high pressure sterilization processes by the advent of Single-Use-Systems (SUS) opens up a new era for the glass electrode based pH sensor. Without the requirement for pretreatment of a pH glass electrode under high temperature and pressure, the classic pH sensing technology delivers a more reliable, more rugged, and more accurate pH measurement. However, the innovative and unique SUS practice also posts new challenges, such as the long shelf-life requirement and the limitation of access to the sensor for calibration once on site,.

At Broadley-James Corporation, a new manufacturing technology has been developed to …

Process Intensification In Biomanufacturing Driven By Advances In Single Use Technologies, Stefan Schmidt

Single-Use Technologies III: Scientific and Technological Advancements

Single use technologies now represent the state of the art in biomanufacturing, but still the full potential of a broad application has not been tapped yet. One strategy that captures most of disposable benefits is process intensification. Here single use systems are optimized to achieve similar output levels as traditional stainless-steel facilities but at much lower capital expenditure, smaller footprints and faster implementation times. This presentation gives an overview on current approaches, describes the opportunities and limits of disposables and delivers recommendations to what should be done by suppliers and users in the near future.

Single Use Plastic Settlers For Clarifying Cell Culture Broth, Selective Removal Of Dead Cells And Affinity Capture Of Antibodies On Protein A Beads, Dhinakar Kompala, Mark Harris, Christopher Glascock, Premsingh Samuel

Single-Use Technologies III: Scientific and Technological Advancements

We have redesigned the successful “inclined settler” technology used as a selective cell retention device in mammalian cell perfusion bioreactor cultures into a more compact and easily scalable design using cylindrical and conical geometries. Through this novel settler design, we have achieved 6 – 10 x more settling area for the same footprint, compared to the traditional multi-plate or lamellar rectilinear scale up design. Using this compact settler design, we have demonstrated significant clarification of the smaller yeast Pichia pastoris cells in continuous harvest or settler effluent stream and high cell densities (700 – 1000 O.D.) in perfusion bioreactors operated …

Purity Testing Protocols For Silicone Tubing, Csilla Kollar, Lise Tan-Sien-Hee

Single-Use Technologies III: Scientific and Technological Advancements

Silicone tubing products are widely used in critical biopharmaceutical processes including final fill and other aseptic operations that require high purity product contact components.

At Dow we have developed reliable and rigorous methods based on the industry standards for particulates, endotoxins and bioburden to test our Platinum-catalyzed silicone tubing family. The tests were conducted per the USP ‹788› Particulate Matter in Injection, the USP ‹85› Bacterial Endotoxins Test, and the ISO 11737-1 Sterilization of medical devices — Microbiological methods — Part 1: Determination of a population of microorganisms on products.

• Method 2 Microscopic Particle Count Test of USP ‹788› was …

Map Based Discovery Of Hydrologic Data In The Hydroshare Collaboration Environment, Zhaokun Xue, Alva Couch, David G. Tarboton

Civil and Environmental Engineering Faculty Publications

Data discovery refers to the process of locating pre-existing data for use in new research. In the HydroShare collaboration environment for water science, there are more than twenty kinds of data that can be discovered, including data from specific sites on the globe, data corresponding to regions on the globe, and data with no geospatial meaning, such as laboratory experiment results. This paper discusses lessons learned in building a data discovery system for HydroShare. This was a surprisingly difficult problem; default behaviors of software components were unacceptable, use cases suggested conflicting approaches, and crafting a geographic view of a large …

Critical Theoretical Frameworks In Engineering Education: An Anti-Deficit And Liberative Approach, Joel A. Mejia, Renata A. Revelo, Idalis Villanueva, Janice Mejia

Engineering Education Faculty Publications

The field of engineering education has adapted different theoretical frameworks from a wide range of disciplines to explore issues of education, diversity, and inclusion among others. The number of theoretical frameworks that explore these issues using a critical perspective has been increasing in the past few years. In this review of the literature, we present an analysis that draws from Freire’s principles of critical andragogy and pedagogy. Using a set of inclusion criteria, we selected 33 research articles that used critical theoretical frameworks as part of our systematic review of the literature. We argue that critical theoretical frameworks are necessary …

“There Is Never A Break”: The Hidden Curriculum Of Professionalization For Engineering Faculty, Idalis Villanueva, Taya Carothers, Marialuisa Di Stefano, Md. Tarique Hasan Kahan

Engineering Education Faculty Publications

The purpose of this exploratory special issue study was to understand the hidden curriculum (HC), or the unwritten, unofficial, or unintended lessons, around the professionalization of engineering faculty across institutions of higher education. Additionally, how engineering faculty connected the role of HC awareness, emotions, self-efficacy, and self-advocacy concepts was studied. A mixed-method survey was disseminated to 55 engineering faculties across 54 institutions of higher education in the United States. Quantitative questions, which centered around the influences that gender, race, faculty rank, and institutional type played in participants’ responses was analyzed using a combination of decision tree analysis with chi-square and …

Physical Modeling Of Flow Nets In Groundwater And Determination Of Hydraulic Conductivity, Hannah Nicholas, Moses Karakouzian

AANAPISI Poster Presentations

The goal of this study is to physically model the paths that water particles take through soil, and estimate hydraulic conductivity for several soil configurations. Water paths, or flow lines, are shown by injecting dye into sand contained in a rectangular acrylic glass tank with a vertical barrier in the center; water is poured on one side of the tank and a pump is used to maintain constant head loss. If flow lines are formed, a flow net is to be drawn using photos of the tank and hydraulic conductivity is to be calculated.

This project consists of four phases: …

Coir Fiber Reinforced Concrete, Jose De La Serna, Moses Karakouzian

AANAPISI Poster Presentations

This poster exhibits the testing and research done on general Portland Cement Concrete with the addition of coir (coconut) fibers as reinforcement. Using the fundamental constituents of cement (water, cement, fine and coarse aggregate) and processed coir fiber, concrete specimens were batched to test for compressive and flexural strengths (per appropriate ASTM standards). Cylindrical and rectangular beam specimens were subjected to loadings until failure occurred. The capacity and modes of failures of the specimens were observed. It was determined that:

• The addition of processed coir fibers decreases the overall strength of general Portland Cement Concrete. • The tested concrete’s …

Simulation Of The Oxygen Reduction Reaction (Orr) Inside The Cathode Catalyst Layer (Ccl) Of Proton Exchange Membrane Fuel Cells Using The Kinetic Monte Carlo Method, Baosheng Bai, Yi-Tung Chen

Mechanical Engineering Faculty Research

In this paper, a numerical model of the kinetic Monte Carlo (KMC) method has been developed to study the oxygen reduction reaction (ORR) that occurs inside the cathode catalyst layer (CCL). Firstly, a 3-D model of the CCL that consists of Pt and carbon spheres is built using the sphere packing method; secondly, an efficient procedure of the proton-oxygen reaction process is developed and simulated. In the proton-oxygen reaction process, all of the continuous movements of protons and oxygen are considered. The maximum reaction distance is determined to be 8 Å. The input pressures of protons and oxygen are represented …

Evaluation Of Heat Efficiency Of Flat Solar Air Heaters, E.S. Abbasov, M.A. Umurzakova

Journal of Tashkent Institute of Railway Engineers

The article discusses the issues of increasing the thermal efficiency of flat solar air collectors. To achieve the goal, the authors propose to use methods of wall intensification of heat transfer (using the diffuser-confusor channels and surfaces with spherical depressions). In the article formulas for calculating the thermal efficiency and the relative thermal performance of the collectors are developed

Theoretical And Experimental Analysis Of The Process Of Defosphoration Of Steel Used For Parts Of The Mobile Composition Of Railway Transport, N.K. Tursunov, Ya.O. Ruzmetov

Journal of Tashkent Institute of Railway Engineers

Analyzed the process of dephosphoration of steel in coreless induction furnaces with a capacity of 6 tones. As the tested metal used steel grade 20GL. An experimental study of the process of steel dephosphoration was carried out in induction furnace with the use of solid slag-forming mixtures

Some Aspects Of Studying The Structure Of Construction Materials By The Method Of Mercury Purometry, Anvar Ishanovich Adilkhodjayev, I.A. Kadirov

Journal of Tashkent Institute of Railway Engineers

The article gives a classification of methods for controlling the porous structure of materials. The parameters of the porosity of aerated concrete were investigated by the method of mercury porosimetry. The method of mercury porosimetry is based on the fact that a liquid that does not wet a solid penetrates into its pores only when exposed to external pressure. The total porosity of aerated concrete according to mercury porosimetry is 49.28%

The Santa Clara, 2018-09-20, Santa Clara University

The Santa Clara

No abstract provided.