Engineering Commons^™

New Microarray Image Segmentation Using Segmentation Based Contours Method, Yuan Cheng

Doctoral Dissertations

The goal of the research developed in this dissertation is to develop a more accurate segmentation method for Affymetrix microarray images. The Affymetrix microarray biotechnologies have become increasingly important in the biomedical research field. Affymetrix microarray images are widely used in disease diagnostics and disease control. They are capable of monitoring the expression levels of thousands of genes simultaneously. Hence, scientists can get a deep understanding on genomic regulation, interaction and expression by using such tools.

We also introduce a novel Affymetrix microarray image simulation model and how the Affymetrix microarray image is simulated by using this model. This simulation …

Microfluidic Devices Applied On Enriching Post –Translational Modified Proteins For Proteomics, Hui Xia

Doctoral Dissertations

In this work, microfluidic devices were developed for enriching post-translational modified proteins. Post-translational modifications (PTM) of proteins play essential roles in cellular physiology and disease. The identification of protein substrates and detection of modification site helps understand PTM-mediated regulation in essential biological pathways and functions in various diseases. However, PTM proteins are typically present only at trace levels, making them difficult to identify in mass spectrometry based proteomics. This work study is about the design, fabrication and testing of the microfluidic device for the enrichment of abundant amount of PTMs. Carbonylated protein is used as a representative PTM to illustrate …

Rheological Study Of Asphalt Binders With A Wax-Based Warm Mix Additive And Its Relationships To Mix Compaction And Rutting, Rajan Saha

Doctoral Dissertations

Although the energy saving Warm Mix Asphalt (WMA) technology that produces asphalt mixes at lower temperatures are around for about a decade, there still exist some concerns that are hindering its wide spread implementation. The overall objective of this study is to investigate if the viscosity reductions at higher temperatures have any impacts on asphalt mix compaction and rutting performances. This study reveals that there exists a critical temperature for each asphalt binder below which viscosity will increase with addition of Sasobit®. Therefore, compaction below the critical temperature can negatively impact density.

For PG 64-22, the critical temperature is 104 …

Ultrasonication Assisted Layer-By-Layer Technology For The Preparation Of Multi-Functional Anticancer Drugs Paclitaxel And Lapatinib, Xingcai Zhang

Doctoral Dissertations

In this dissertation, ultrasonication assisted Layer-by-Layer (LbL) technology for the preparation of multifunctional poorly water-soluble anticancer drug nanoparticles, paclitaxel and lapatinib, has been developed. Many FDA approved drugs are very low soluble in water; therefore, it is very difficult to load and control their release and targeting efficiently, which greatly confines their application. The development of this method will pave the way for the development and application of those low soluble anticancer drugs.

In the first part of this dissertation, the first approach for powerful ultrasonication, the top-down approach (sonicating bulk drug crystals in polyelectrolyte solution), was successfully applied for …

Using Power-Law Properties Of Social Groups For Cloud Defense And Community Detection, Justin L. Rice

Doctoral Dissertations

The power-law distribution can be used to describe various aspects of social group behavior. For mussels, sociobiological research has shown that the Lévy walk best describes their self-organizing movement strategy. A mussel's step length is drawn from a power-law distribution, and its direction is drawn from a uniform distribution. In the area of social networks, theories such as preferential attachment seek to explain why the degree distribution tends to be scale-free. The aim of this dissertation is to glean insight from these works to help solve problems in two domains: cloud computing systems and community detection.

Privacy and security are …

Engineering Microenvironments To Modulate Calcium Information Processing In Neuronal Cells, Kinsey Cotton Kelly

Doctoral Dissertations

Tissue engineered microenvironments were constructed to test the effects glial cells have on calcium information processing, and to mimic conditions in vivo for tumor invasion and residual cancer after resection of tumor. Submaximal, nM, glutamate (GLU) stimuli were applied to the engineered environments, and the resulting calcium dynamic behavior of neuronal cells was measured to help predict and interpret chaotic systems in the experimental realm. Calcium is a key signaling ion which signals through the N-methyl-D-aspartate (NMDA) glutamate receptor on the neuronal membrane. GLU binding to the NMDA receptor (NMDAR) causes a large and dynamic increase in neuronal intracellular calcium. …

Variability Of Extreme Precipitation In Coastal River Basins Of The Southern Mexican Pacific Region, Nancy Perez-Morga, Thomas Kretzschmar, Tereza Cavazos, Stephen V. Smith, Francisco Munoz-Arriola

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Extreme wet and dry years (± 1 standard deviation, respectively), as well as the top 95 percentile (P95) of daily precipitation events, derived from tropical cyclone (TC) and nontropical cyclone (NTC) rainfall, were analyzed in coastal river basins in Southern Oaxaca, Mexico (Río Verde, Río Tehuantepec, and the Southern Coast). The study is based on daily precipitation records from 47 quality-controlled stations for the 1961 to 1990 period and TC data for the Eastern Tropical Pacific (EPAC). The aim of this study was to evaluate extreme (dry and wet) trends in the annual contribution of daily P95 precipitation events and …

Study Of The Effects Of Changing Physiological Conditions On Dielectric Properties Of Breast Tissues, Abas Sabouni, Camerin Hahn, Sima Noghanian, Edward Sauter, Tim Weiland

Electrical Engineering Faculty Publications

This paper addresses the changes in the physical characteristics (temperature and water/blood content) of breast tissue under different physiological conditions. We examined ex vivo specimens of breast tissue excised at the time of surgery to study the effects of physiological conditions on dielectric properties. We observed that the dielectric properties strongly depend on tissue physiological state. When the biological tissues undergo physiological changes, such as those due to disease or those induced by external changes such as variations in the environmental temperature, the microscopic processes deviate from their normal state and impact the overall dielectric properties. This suggests that microwave …

Flexible Supercapacitor Sheets Based On Hybrid Nanocomposite Materials, Qiang Liu, Osama Nayfeh, Munir H. Nayfeh, Siu-Tung Yau

Electrical and Computer Engineering Faculty Publications

Nanomaterials with quasi-zero, one and two dimensionalities, including silicon nanoparticles, carbon nanotubes, titanium oxide particles and graphene flakes, have been incorporated into the conducting polymer polyaniline to form nanocomposite materials for making flexible supercapacitor sheets. Characterization of the capacitor sheets showed that the inclusion of the nanomaterials in polyaniline has significantly improved the energy and power capabilities of the capacitor. In particular, a specific capacitance of 477.1 F/g has been obtained. The important properties of carbon nanotubes of improving the charge storage and reducing the resistance of the nanocomposite material and hence to enhance the power capability of the capacitor …

2013-2014 Kentucky & Tennessee Tobacco Production Guide, Kenneth W. Seebold, Robert C. Pearce, William A. Bailey, Lowell P. Bush, Jonathan D. Green, Robert D. Miller, Laura A. Powers, William M. Snell, Lee H. Townsend, Mark Purschwitz, Larry G. Wells, John H. Wilhoit, Margarita Velandia, Steve Bost, Gene Burgess, Frank Hale, Darrell Hensley, Paul Denton, Neil Rhodes

Agriculture and Natural Resources Publications

Tobacco growers in Kentucky and Tennessee have faced many challenges during the past two years, including weather, labor availability, and rising production costs. However, there have been opportunities as well. Some buying stations have closed or moved, but new buyers have come into the market and contract volumes have been stable overall. Good quality burley tobacco is in relatively short supply, and this has offered opportunities to those who have been able to overcome weather and labor challenges and produce good crops. These changes have had different impacts in different regions of both states, based largely on where buying stations …

A Note On The Occurrence Of Yttrium And Rare Earth Elements In Coal Combustion Products, James C. Hower, Shifeng Dai, Vladimir V. Seredin, Lei Zhao, Irena J. Kostova, Luis F.O. Silva, Sarah M. Mardon, Gulbin Gurdal

Coal Combustion and Gasification Products Journal Archive

There is an international need for a variety of lanthanide elements (rare earth elements, or REEs) in modern electronic and related components. This has led to a desire to broaden production from both previously productive locations and to expand production to new sources, possibly including coal-derived fly ash. The concentration of lanthanides in coal-combustion fly ash depends on a number of factors, one of the most important being the concentration of the elements in the feed coal. Unlike some elements, such as Zn and As, the REE concentration is largely not a function of element volatility. In this study, we …

Fouling Intensity Of Three Indian Coals, S. Balakrishnan, R. Nagarajan

Coal Combustion and Gasification Products Journal Archive

Ash particles can arrive at heat-transfer surfaces either in solid (solidified) state or in molten state, depending on the factors such as location inside the boiler, temperature, and residence time. In this study, arrival of ash particles in solid state, which can occur in the convective section of the boiler, was considered. Apart from the inertial force that drives particles .10 mmto the surface, the low-melting inorganic alkali vapors play a vital role in deposit formation by promoting sticking. Chemical equilibrium calculations were performed to calculate the equilibrium composition of flue gas, as well as the amount of condensable inorganic …

A Computational Approach To Simulating The Performance Of A 24-Hour Solar-Fuel Cell-Hydrogen Electric Power Plant, Michael K. Gustafson

Browse all Theses and Dissertations

World energy demand has risen from about 375 exajoules (EJ) in 1990 to around 600 EJ today. The Energy Information Administration predicts that by the year 2035, this figure will rise to around 800 EJ. This places large stresses on the electric generation infrastructure. Increasingly this demand is being met by renewable energy sources. There are several reasons this is the case. The prices of renewables are dropping quickly and reaching grid parity in more regions. Utilizing renewable energy generation can help achieve energy security: adverse weather or military conflicts are less likely to impact supply routes when energy is …

Synthesis And Characterization Of Graphene Oxide/Sulfur Nanocomposite For Lithium-Ion Batteries, Aaron Joseph Blake

Browse all Theses and Dissertations

The growing need for clean and efficient energy storage systems has recently peaked due to concerns of climate change and increased global energy consumption. However, efficiently integrating renewable resources such as solar and wind energy into society will require a complex electrical energy storage (EES) system capable of storing and expending significant amounts of energy. A battery based on the lithium/sulfur couple can yield a theoretical specific energy of 2600Wh/kg, which is about five times higher than that offered by present Li-ion batteries, and hence, is a promising and attractive technology. Despite recent developments in addressing various issues inherent to …

Consequence Based Procedure For Description Logics With Self Restriction, Cong Wang, Pascal Hitzler

Computer Science and Engineering Faculty Publications

We present a consequence based classification procedure for the description logics with self restriction constructor. Due to the difficulty of constructing a concept inclusion model for self restriction, we use a different proof by showing that all the completion rules can simulate all the corresponding ordered resolution inferences.

Reliability-Based Fatigue Assessment Of Mast-Arm Sign Support Structures, Joseph A. Diekfuss

Dissertations (1934 -)

Over the past few decades, there have been issues of poor fatigue performance (the main failure mechanism) of the welded, tube-to-transverse plate connections within sign support structures. Review of the literature has indicated that a considerable amount of research has been devoted to identifying the structural response characteristics of these signs. Others have tried to identify how these connections may be repaired, retrofitted or simply better designed to sustain longer fatigue lives. However, little attention has been given to using a systematic reliability-based approach to assess the risk of fatigue-induced fracture in these structures.

Using a reliability-based approach to solve …

Compliance In The Ether: Cloud Computing, Data Security And Business Regulation, J. Nicholas Hoover

Journal of Business & Technology Law

No abstract provided.

Scene Understanding For Real Time Processing Of Queries Over Big Data Streaming Video, Alexander Aved

Electronic Theses and Dissertations

With heightened security concerns across the globe and the increasing need to monitor, preserve and protect infrastructure and public spaces to ensure proper operation, quality assurance and safety, numerous video cameras have been deployed. Accordingly, they also need to be monitored effectively and efficiently. However, relying on human operators to constantly monitor all the video streams is not scalable or cost effective. Humans can become subjective, fatigued, even exhibit bias and it is difficult to maintain high levels of vigilance when capturing, searching and recognizing events that occur infrequently or in isolation. These limitations are addressed in the Live Video …

Simulation-Based Cognitive Workload Modeling And Evaluation Of Adaptive Automation Invoking And Revoking Strategies, Christina Rusnock

Electronic Theses and Dissertations

In human-computer systems, such as supervisory control systems, large volumes of incoming and complex information can degrade overall system performance. Strategically integrating automation to offload tasks from the operator has been shown to increase not only human performance but also operator efficiency and safety. However, increased automation allows for increased task complexity, which can lead to high cognitive workload and degradation of situational awareness. Adaptive automation is one potential solution to resolve these issues, while maintaining the benefits of traditional automation. Adaptive automation occurs dynamically, with the quantity of automated tasks changing in real-time to meet performance or workload goals. …

A Comparative Evaluation Of Fdsa,Ga, And Sa Non-Linear Programming Algorithms And Development Of System-Optimal Methodology For Dynamic Pricing On I-95 Express, Don Graham

Electronic Theses and Dissertations

As urban population across the globe increases, the demand for adequate transportation grows. Several strategies have been suggested as a solution to the congestion which results from this high demand outpacing the existing supply of transportation facilities. High –Occupancy Toll (HOT) lanes have become increasingly more popular as a feature on today’s highway system. The I-95 Express HOT lane in Miami Florida, which is currently being expanded from a single Phase (Phase I) into two Phases, is one such HOT facility. With the growing abundance of such facilities comes the need for indepth study of demand patterns and development of …

Interdiffusion Study Of Mg-Aa6061 System, Mian Fu

Electronic Theses and Dissertations

Magnesium (Mg) is a light-weight metal that has extraordinary physical and chemical properties for many potential applications in automobile, military, and electronics. Aluminum alloys, because of its light-weight, high strength and corrosion resistance have a wide range of commercial applications. Given these two, sometime competing, alloy systems, there are now many applications where the metallurgical compatibility of Mg- and Al-alloys are required for engineering applications. One such case is the development of diffusion barrier for U-Mo metallic fuel in Al-alloy cladding, where Mg, with its complete immiscibility with U and Mo is being considered as the diffusion barrier. While negligible …

Hydromorphology Of The Econlockhatchee River, John Baker

Electronic Theses and Dissertations

Climate change and human activities alter the hydrologic systems and exerted global scale impacts on our environment with significant implications for water resources. Climate change can be characterized by the change of precipitation and temperature, and both precipitation pattern change and global warming are associated with the increase in frequency of flooding or drought and low flows. With increasing water demand from domestic, agricultural, commercial, and industrial sectors, humans are increasingly becoming a significant component of the hydrologic cycle. Human activities have transformed hydrologic processes at spatial scales ranging from local to global. Human activities affecting watershed hydrology include land …

Understanding The Capabilities And Limitations Of Advanced Interactive M&S: A Cricothyroidotomy Simulation Case Study, Lillian Campbell-Wynn

Electronic Theses and Dissertations

Simulation for surgical education and training is increasingly perceived as a valuable contribution to traditional teaching methods providing a structured learning experience. Surgical simulations allow surgeons to practice tactics, techniques and procedures numerous times without the cost, limitations and ethical problems of using cadaver-based models. The goal of this research is to advance the use of modeling and simulation in support of emergency medical training. This research explores questions identified through a case study of two different modeling and simulation techniques – virtual reality and mannequins - in the support of combat emergency medical education and training. To reduce the …

Fast-Response Liquid Crystals For Photonic And Display Applications, Jie Sun

Electronic Theses and Dissertations

Liquid crystal devices are attractive for many applications such as information displays, spatial light modulators and adaptive optics, because their optical properties are electrically tunable. However, response time of liquid crystal devices is a serious concern for many applications especially for those who require large phase modulation (≥2π). This is because a thick LC layer is usually needed to achieve a large phase shift while the response time of a nematic LC is highly determined by the cell gap.

Optically Induced Forces In Scanning Probe Microscopy, Dana Kohlgraf-Owens

Electronic Theses and Dissertations

The focus of this dissertation is the study of measuring light not by energy transfer as is done with a standard photodetector such as a photographic film or charged coupled device, but rather by the forces which the light exerts on matter. In this manner we are able to replace or complement standard photodetector-based light detection techniques. One key attribute of force detection is that it permits the measurement of light over a very large range of frequencies including those which are difficult to access with standard photodetectors, such as the far IR and THz. The dissertation addresses the specific …

High Flux Isolated Attosecond Pulse Generation, Yi Wu

Electronic Theses and Dissertations

This thesis outlines the high intensity tabletop attosecond extreme ultraviolet laser source at the Institute for the Frontier of Attosecond Science and Technology Laboratory. First, a unique Ti:Sapphire chirped pulse amplifier laser system that delivers 14 fs pulses with 300 mJ energy at a 10 Hz repetition rate was designed and built. The broadband spectrum extending from 700 nm to 900 nm was obtained by seeding a two stage Ti:Sapphire chirped pulse power amplifier with mJ-level white light pulses from a gas filled hollow core fiber. It is the highest energy level ever achieved by a broadband pulse in a …

Peak Power Scaling Of Nanosecond Pulses In Thulium Based Fiber Lasers, Christian Gaida

Electronic Theses and Dissertations

Thulium based fiber lasers represent a promising alternative for pulse energy scaling and high peak power generation with ytterbium based systems at 1µm. Advantages of thulium arise from the operation at longer wavelengths and a large gain bandwidth (1.8-2.1µm). Nonlinear effects, such as self phase modulation, stimulated Raman scattering and stimulated Brillouin scattering generally limit peak power scaling in fiber lasers. The longer wavelength of thulium fiber lasers and large mode field areas can significantly increase the nonlinear thresholds. Compared to 1µm systems, thulium fiber lasers enable single mode guidance for two times larger mode field diameter in step index …

Valence-Length Correlations For Chemical Bonds From Atomic Orbital Exponents, F. D. Hardcastle

Journal of the Arkansas Academy of Science

Pauling’s empirical bond valence-length correlation has proven valuable because it offers a quick and convenient way of checking and evaluating molecular structures and determining oxidation states from measured bond lengths. In this study, a simplified quantum-mechanical approach was used to derive Pauling’s empirical bond valence-length relationship by considering overlap of hydrogen-like orbitals. An expression for the b “empirical” fitting parameter was derived in terms of atomic-orbital exponents. A new set of orbital exponents is presented using published atomic/covalent radii and a continuous function for the effective principal quantum. The b parameters calculated from the orbital exponents are consistent with bond …

Swcc And Clay Mineralogy Based Models For Realistic Simulation Of Swell Behavior Of Expansive Soils, Aravind Pedarla

Civil Engineering Dissertations - Archive

Predicting the volume change behavior of expansive soils has been a major challenge to the engineers in the past few years, due to the intricate couple effects caused by different soil composition parameters toward swelling. Hence, the behavior of an expansive soil is better understood with its inherent composition parameters. For this research, eight natural expansive clays have been sampled from different active swell zones across The United States. Basic soil classification was performed on these soil to identify the high plasticity and low plasticity clays. The swell behavior of these clays are studied using different testing methods and initial …

Non-Linear Finite Element-Based Material Constitutive Law For Zero Slump Steel Fiber Reinforced Concrete Pipe Structures, Alena Mikhaylova

Civil Engineering Dissertations - Archive

This study presents a comprehensive investigation of performance and behavior of steel-fiber reinforced concrete pipes (SFRCP). The main goal of this study is to develop the material constitutive model for steel fiber reinforced concrete used in dry-cast application. To accomplish this goal a range of pipe sizes varying from 15 in. (400 mm) to 48 in. (1200 mm) in diameter and fiber content of 0.17%, 0.25%, 0.33%, 0.5%, 0.67% and 83% by volume were produced. The pipes were tested in three-edge bearing condition to obtain the load-deformation response and overall performance of the pipe. The pipes were also subjected to …