Single-Cell Measurements Of Ige-Mediated Fcεri Signaling Using An Integrated Microfluidic Platform, Yanli Liu, Dipak Barua, Peng Liu, Bridget S. Wilson, Janet M. Oliver, William S. Hlavacek, Anup K. Singh

Chemical and Biochemical Engineering Faculty Research & Creative Works

Heterogeneity in responses of cells to a stimulus, such as a pathogen or allergen, can potentially play an important role in deciding the fate of the responding cell population and the overall systemic response. Measuring heterogeneous responses requires tools capable of interrogating individual cells. Cell signaling studies commonly do not have single-cell resolution because of the limitations of techniques used such as Westerns, ELISAs, mass spectrometry, and DNA microarrays. Microfluidics devices are increasingly being used to overcome these limitations. Here, we report on a microfluidic platform for cell signaling analysis that combines two orthogonal single-cell measurement technologies: on-chip flow cytometry …

Low-Complexity Turbo Detection For Single-Carrier Low-Density Parity-Check-Coded Multiple-Input Multiple-Output Underwater Acoustic Communications, Longbao Wang, Jun Tao, Chengshan Xiao, T. C. Yang

Electrical and Computer Engineering Faculty Research & Creative Works

A low-complexity turbo detection scheme is proposed for single-carrier multiple-input multiple-output (MIMO) underwater acoustic (UWA) communications using low-density parity-check (LDPC) channel coding. The low complexity of the proposed detection algorithm is achieved in two aspects: first, the frequency-domain equalization technique is adopted, and it maintains a low complexity irrespective of the highly dispersive UWA channels; second, the computation of the soft equalizer output, in the form of extrinsic log-likelihood ratio, is performed with an approximating method, which further reduces the complexity. Moreover, attributed to the LDPC decoding, the turbo detection converges within only a few iterations. The proposed turbo detection …

Performance Measures For Local Agency Traffic Signals, Jay Grossman, Darcy M. Bullock

Indiana Local Technical Assistance Program (LTAP) Technical Reports

The project focused on accelerating implementation of research recommendations emerging from traffic signal research projects conducted by the National Cooperative Research Program and Indiana Department of Transportation. This project developed specification language that was subsequently used in procurements in Elkhart County, IN; Lafayette, IN; and Morgantown, WV. The performance measures specified in Appendix A and subsequently deployed in Elkhart County, IN, and Lafayette, IN, were the first commercial deployment of real-time traffic signal performance measures recommended by NCHRP 3-79a. The deployment in Lafayette, IN, received an ACEC award in March 2012. In addition to accelerating implementation, this research project examined …

2013 Directory Of Indiana State, County, City And Town Officials Responsible For Road And Street Work, Indiana Ltap

Indiana Local Technical Assistance Program (LTAP) Directory

Contact information for local agency officials and employees responsible for Indiana’s local road infrastructure.

2012 Statewide Bridge Sufficiency Rating Report, Indiana Ltap, Indiana Department Of Transportation, Indiana Department Of Local Government Finance

Indiana Local Technical Assistance Program (LTAP) Publications

The main purpose of this report is to provide bridge sufficiency rating information for the bridges in each county to county officials as a convenient starting point for formulating a long-range plan to cope with their county bridge deficiencies. This information results from the data generated by the national Bridge Inspection Standards (NBIS) program authorized by the 1968 Federal-Aid Highway Act.

2013 (Spring) Ensi Informer Magazine, Morehead State University. Engineering Sciences Department

ENSI Informer Magazine Archive

The ENSI Informer Magazine published in the spring of 2013.

Bs News March/April

Building Services Engineering

No abstract provided.

Development Of Novel Fiber Optic Humidity Sensors And Their Derived Applications, Jinesh Mathew

Doctoral

The main focus of this thesis is on the design and development of novel fiber optic devices for relative humidity (RH) sensing with emphasis on high sensitivity, a wide humidity range, low temperature dependence, fast response time and good stability.
Novel RH sensors based on fiber bends are fabricated by coating the surface of the buffer stripped bent fiber with selected hygroscopic materials such as Polyethylene oxide or Agarose. It is shown that the Polyethylene oxide coated device has a high sensitivity in a narrow RH range while the Agarose coated fiber bend shows a linear RH sensitivity in a …

Note: A Simple Thermal Gradient Annealing Unit For The Treatment Of Thin Films, C. J. Metting, Johnathan K. Bunn, Ellen A. Underwood, Yihao Zhu, G. Koley, T. Crawford, Jason R. Hattrick-Simpers

Faculty Publications

A gradient annealing cell has been developed for the high-throughput study of thermalannealing effects on thin-film libraries in different environments. The inexpensive gradientannealing unit permits temperature gradients as large as 28 °C/mm and can accommodate samples ranging in length from 13 mm to 51 mm. The system was validated by investigating the effects of annealing temperature on the crystallinity, resistivity, and transparency of tin-doped indium oxide deposited on a glass substrate by magnetron sputtering. The unit developed in this work will permit the rapid optimization of materials properties such as crystallinity, homogeneity, and conductivity across a variety of applications.

Three-Dimensional Engineered Matrix To Study Cancer Stem Cells And Tumorsphere Formation: Effect Of Matrix Modulus, Xiaoming Yang, Samaneh Kamali Sarvestani, Seyedsina Moeinzadeh, Xuezhong He, Esmaiel Jabbari

Faculty Publications

Maintenance of cancer stem cells (CSCs) is regulated by the tumor microenvironment. Synthetic hydrogels provide the flexibility to design three-dimensional (3D) matrices to isolate and study individual factors in the tumor microenvironment. The objective of this work was to investigate the effect of matrix modulus on tumorsphere formation by breast cancer cells and maintenance of CSCs in an inert microenvironment without the interference of other factors. In that regard, 4T1 mouse breast cancer cells were encapsulated in inert polyethylene glycol diacrylate hydrogels and the effect of matrix modulus on tumorsphere formation and expression of CSC markers was investigated. The gel …

Adaptation Of Classical Tidal Harmonic Analysis To Nonstationary Tides, With Application To River Tides, Pascal Matte, David A. Jay, Edward D. Zaron

Civil and Environmental Engineering Faculty Publications and Presentations

One of the most challenging areas in tidal analysis is the study of nonstationary signals with a tidal component, as they confront both current analysis methods and dynamical understanding. A new analysis tool has been developed, NS_TIDE, adapted to the study of nonstationary signals, in this case, river tides. It builds the nonstationary forcing directly into the tidal basis functions. It is implemented by modification of T_TIDE; however, certain concepts, particularly the meaning of a constituent and the Rayleigh criterion, are redefined to account for the smearing effects on the tidal spectral lines by nontidal energy. An error estimation procedure …

Women's High Heel Discomfort Analysis And Prototype Solution, Kevin Blakley, Annamarie Kepple

Industrial and Manufacturing Engineering

ABSTRACT

WOMEN’S HIGH HEEL DISCOMFORT ANALYSIS AND SOLUTION

Kevin Blakley and Annamarie Kepple

Women love high heeled shoes, but this love comes at the cost of comfort. Wearing high heels causes a lot of pain and, in the long term, health complications. This project tries to overcome the problem by ultimately making high heels more comfortable. In order to do this, one must first understand the reasons that high heels are uncomfortable, such as arch angle, heel height, and the changes in walking motion. Once there is a true understanding of the factors that contribute to high heel discomfort, the …

Film Cooling In Fuel Rich Environments, Jacob J. Robertson

Theses and Dissertations

The ultra compact combustor is a high performance gas turbine design concept that portends reduced weight for future weapons platforms. A natural outcome of the design is the continual presence of fuel-rich air in the turbine component of the engine. Because modern cooling schemes for hot section turbine blades involve injecting cool, oxygen-rich air adjacent to the surface, the potential arises for reaction with the unconsumed radicals in the mainstream ow and augmented heat transfer to the blade. This outcome is contrary to the purpose of film cooling, and can lead to early life-cycle turbine failure. This study examined the …

Design And Testing Of An H2/O2 Predetonator For A Simulated Rotating Detonation Engine Channel, Stephen J. Miller

Theses and Dissertations

A study is presented on the relationship between a pre-detonator and a detonation channel of an RDE. Testing was conducted on a straight narrow channel made of clear polycarbonate windows connected to an H2/O2 pre-detonator to simulate the RDE initiation scheme and allow for flow visualization. A comparison is made on decoupling distance and wave velocities for a range of pre-detonator designs, inclination angles, equivalence ratios and geometries placed within the simulated channel. Regardless of inclination angle or equivalence ratio the detonation wave decoupled within 25 mm from the pre-detonator exit into the channel. A step change in diameter 25 …

Modelling And Hazard Analysis For Contaminated Sediments Using Stamp Model And Stpa Tool, Karim Hardy, Franck Guarnieri

Publications

The goal of this article is dual: first, introducing a new model of accident named STAMP (systems-theoretic accident modeling and processes); then applying the model to an innovative process for the treatment of contaminated substances and the re-use of treated substances. This article is a demonstration for a need of a new tool to take into account hazards and safety within socio-technical systems.

Robust Controller Design For An Electrostatic Micromechanical Actuator, Lili Dong, Jason Edwards

Electrical and Computer Engineering Faculty Publications

In this paper, a robust feedback controller is developed on an electrostatic micromechanical actuator to extend the travel range of it beyond pull-in limit. The actuator system is linearized at multiple operating points, and the controller is constructed based on the linearized model. Two kinds of controller designs are developed for set-point tracking of the actuator despite the presences of sensor noise and external disturbance. One of them is a regular fourth order Active Disturbance Rejection Controller (ADRC) and is able to achieve 97% of the maximum travel range. And the other one is a novel multi-loop controller with a …

An Active Disturbance Rejection Based Approach To Vibration Suppression In Two‐Inertia Systems, Shen Zhao, Zhiqiang Gao

Electrical and Computer Engineering Faculty Publications

This study concerns the resonance problems found in motion control, typically described in a two‐inertia system model as compliance between the motor and the load. We reformulate the problem in the framework of active disturbance rejection control (ADRC), where the resonance is assumed to be unknown and treated as disturbance, estimated and mitigated. This allows the closed‐loop bandwidth to go well beyond the resonant frequency, which is quite difficult using existing methods. In addition, such level of performance is achieved with minimum complexity in the controller design and tuning: no parameter estimation or adaptive algorithm is needed, and the controller …

Me-Em Enewsbrief, March 2013, Department Of Mechanical Engineering-Engineering Mechanics, Michigan Technological University

Department of Mechanical and Aerospace Engineering eNewsBrief

No abstract provided.

Analyzing Communication Performance: A Comparison Of Native-English Speakers And Non-Native English Speakers, Tami Marie Gibbs

Doctoral Dissertations and Master's Theses

The purpose of this thesis was to research how well native and non-native English speakers proficiently communicated using learned Air Traffic Control (ATC) phraseology, and how well they communicated using plain phraseology. The participants in this study were Embry-Riddle Aeronautical University (ERAU) flight students at the Daytona Beach campus. This study used a sample of students from English-speaking countries and a sample of students from non-English speaking countries. A quantified study of the types of communication errors was conducted by listening to a pre-defined amount of voice communications between the Daytona Beach International Airport tower facility and ERAU flight student …

Pre-Exposure Training As A Means To Reduce Vection Induced Symptoms Of Motion Sickness, Nicholas James Stapleton

Doctoral Dissertations and Master's Theses

The focus of this study was designed to empirically examine the effect of inducing mild motion sickness as an ameliorative to a more severe motion sickness exposure. Twenty-seven participants were selected for this study based upon their susceptibility to motion sickness, that is, only people who were determined to be motion susceptible were tested. All participants were exposed to a motion sickness-inducing environment. Eighteen participants were trained to adapt to motion sickness by exposure to a milder motion sickness-inducing environment, ending either 6 hours or 24 hours prior to the more severe motion test environment.

Participants during the pre-exposure experimental …

Synthetic Array Processing Of Ocean Ambient Noise For Higher Resolution Seabed Bottom Loss Estimation, Martin Siderius, Lanfranco Muzi, Chris H. Harrison, Peter L. Nielsen

Electrical and Computer Engineering Faculty Publications and Presentations

Predicting transmission loss in the ocean often strongly depends on the bottom loss. Bottom loss can be estimated using ocean noise and vertical array beam-forming [Harrison and Simons, J. Acoust. Soc. Am. 112, 1377–1389 (2002)]. With finite length arrays, the bottom loss estimate using this method can be smoothed due to beam widths. This paper describes how the noise coherence function can be synthetically expanded, which is similar to extending the length of an array. A full wave ocean noise model is used to demonstrate, in simulation, how this leads to improvements in the resolution of bottom loss estimates.

Tracking Knee Joint Functional Axes Through Tikhonov Filtering And Plűcker Coordinates, Wangdo Kim, Yoon-Hyuk Kim, António P. Veloso, Sean S. Kohles

Mechanical and Materials Engineering Faculty Publications and Presentations

Researchers have reported several compensation methods to estimate bone and joint position from a cluster of skin-mounted markers as influenced by Soft Tissue Artifacts (STA). Tikhonov Regularization Filtering (TRF) as a means to estimate Instantaneous Screw Axes (ISA) was introduced here as a means to reduce the displacement of a rigid body to its simplest geometric form. Recent studies have suggested that the ISA of the knee, i.e., Knee Functional Axes (KFA), might be closely connected to the estimation of constraint forces such as those due to medial and lateral connective tissues. The estimations of ISAs were known to be …

An In Situ Experimental Study Of Grain Growth In A Nanocrystalline Fe91ni8zr1 Alloy, Hasan Kotan, Kris A. Darling, Mostafa Saber, Ronald O. Scattergood, Carl C. Koch

Mechanical and Materials Engineering Faculty Publications and Presentations

Grain growth and microstructural evolution of thermally stabilized Fe91Ni8Zr1 were investigated by in situ and ex situ studies. Our investigations suggest that the microstructural evolution is fairly slow and the microstructure shows stabilization up to about 700 °C. Above this temperature, a certain fraction of grains grow abnormally into the nanocrystalline matrix, resulting in a bimodal microstructure and causing the complete loss of thermal stability. The reason for abnormal grain growth and the loss of thermal stability is identified as the appearance of the fcc γ-phase and consequent reduction in the total area of grain boundaries and the overall stored …

Axisymmetric Air Augmented Methanol/Gox Rocket Mixing Duct Experimental Thrust Study, Kyle Jacob Johnson

Master's Theses

A hot-flow axisymmetric Air Augmented Rocket (AAR) test apparatus was constructed to test various mixing duct configurations at static conditions. Primary flow for the AAR was provided through a liquid methanol-gaseous oxygen bipropellant rocket. Experimental thrust measurements were recorded and propellant mass flow rates and chamber conditions were calculated using an iterative solver dependant on recorded propellant line stagnation pressures. Primary rocket flow produced thrust ranging from 14 to 17.9lbf. Primary mass flow rate through testing ranged from 0.071 to 0.085lbm/s with calculated chamber pressures between 298-362psia. Calculated primary flow velocity ranged from 6,600ft/s to 8,000ft/s depending on propellant pressure …

Effects Of Ovariectomy And Anatomical Location On Osteonal Encroachment In Adult Cortical Ovine Bone, Paige Brell Ryan

Master's Theses

The purpose of this study is to further quantify adult ovine ovariectomized bone for new remodeling characteristics to obtain a better understanding of how remodeling is occurring and the effectiveness of this animal model for the study of postmenopausal osteoporosis. Postmenopausal osteoporosis is a major health concern and animal models to test new treatment options are needed. The ovine model is a good option because the ewes undergo Haversian remodeling, are a large sized animal, and have a similar hormone profile to humans. Ewes, however, do not undergo a natural menopause, so an ovariectomy surgery was conducted in the sheep …

Modeling Flood Reduction Scenarios For A Small Coastal Community, Evan J. Perez

Master's Theses

The Arroyo Grande Creek Watershed, an approximately 170 mi² watershed located on the central coast of California, drains to the Pacific Ocean via the Arroyo Grande Creek that passes through several coastal cities including the community of Oceano. At the mouth of the Creek is the Arroyo Grande Lagoon, which is connected to another lagoon known as the Oceano Lagoon, by a tidal flap-gate whose hydraulics is a function of water levels in the two lagoons. Historically the Oceano Lagoon has played a part in floods that have occurred in the community of Oceano. The most recent flooding occurred …

System Identification Of A Bridge-Type Building Structure, Pablo D. Ramos Jr.

Master's Theses

The Bridge House is a steel building structure located in Poly Canyon, a rural area inside the campus of California Polytechnic State University, San Luis Obispo. The Bridge House is a one story steel structure supported on 4 concrete piers with a lateral force resisting system (LFRS) composed of ordinary moment frames in the N-S direction and braced frames in the E-W direction and vertically supported by a pair of trusses. The dynamic response of the Bridge House was investigated by means of system identification through ambient and forced vibration testing. Interesting findings such as diaphragm flexibility, foundation flexibility and …

Design And Performance Of Circulation Control Geometries, Rory Martin Golden

Master's Theses

With the pursuit of more advanced and environmentally-friendly technologies of today’s society, the airline industry has been pushed further to investigate solutions that will reduce airport noise and congestion, cut down on emissions, and improve the overall performance of aircraft. These items directly influence airport size (runway length), flight patterns in the community surrounding the airport, cruise speed, and many other aircraft design considerations which are setting the requirements for next generation aircraft. Leading the research in this movement is NASA, which has set specific goals for the next generation regional airliners and has categorized the designs that meet the …

Dc, Rf, And Thermal Characterization Of High Electric Field Induced Degradation Mechanisms In Gan-On-Si High Electron Mobility Transistors, Matthew Anthony Bloom

Master's Theses

Gallium Nitride (GaN) high electron mobility transistors (HEMTs) are becoming increasingly popular in power amplifier systems as an alternative to bulkier vacuum tube technologies. GaN offers advantages over other III-V semiconductor heterostructures such as a large bandgap energy, a low dielectric constant, and a high critical breakdown field. The aforementioned qualities make GaN a prime candidate for high-power and radiation-hardened applications using a smaller form-factor. Several different types of semiconductor substrates have been considered for their thermal properties and cost-effectiveness, and Silicon (Si) has been of increasing interest due to a balance between both factors.

In this thesis, the DC, …

Hydrologic Modeling Of The San Joaquin Valley Watershed For Purposes Of Nitrate Analysis, Stephen Carl Clayton

Master's Theses

The San Joaquin Valley is regarded as one of the most productive agricultural regions in the world. This extensive agriculture has, however, caused extensive pollution of both ground water and surface water. This thesis develops a hydrologic model of the surface and ground waters of the San Joaquin Valley. Such modeling is useful in the development and implementation of water quality regulations such as Total Maximum Daily Loads (TMDLs). A properly validated watershed simulation model can supplement data collection and can account for watershed characteristics including topography, soils, climate, land cover, anthropogenic activities, as well as simulate watershed responses including …