Engineering Commons^™

Attachment Kinetics And Differentiations Of Osteoblasts On Different Biomaterials, U. Meyer, D. H. Szulczewski, K Moller, H. Heide, D. B. Jones

Cells and Materials

Primary bovine osteoblast cell cultures were used to study the response of osteoblasts ton three different biomaterials, ionomeric cement (IC), tri-calcium phosphate poly-L-lactic acid composite foil (TCP) and poly-L-lactate-polycitric acid composite foil (PLA). The cells which grew on the materials produced all typical bone matrix proteins and were osteoblast-Iike as shown by immuno-staining. Analysis of the cell attachment kinetics revealed significant differences within the first 7 hours between the various materials. The best rate of cell attachment was found on the IC surface, followed by the TCP and then the PLA surface. The kinetics of attach ment appeared to be …

Cell-Degradation Of Calcium Phosphate Ceramics, P. Frayssinet, N. Rouquet, F. Tourenne, J. Fages, D. Hardy, G. Bonel

Cells and Materials

Calcium phosphate ceramics are used in bone surgery under different forms: dense or porous ceramic s as bone substitute, thin ceramic coatings on metallic implants as an osseointegration enhancer. Their degradation depends on their physico-chemical properties and particularly on their chemical composition. Natural calcium phosphates of bone are degraded by mononuclear or multinuclear cells and the extracellular matrix induces the differentiation of the degrading-cells. Hydroxyapatite, which is one of the most used calcium phosphates , is known as a low degradation material. However, the histological analysis of implanted HA-materials both in animals and in humans showed that a cellular degradation …

Analysis Of The Bony Interface With Various Types Of Hydroxyapatite In Vitro, J. D. De Bruijn, J. S. Flach, K. De Groot, C. A. Van Blitterswijk, J. E. Davies

Cells and Materials

Rat bone marrow cells, capable of forming bonelike tissue, were cultured on three types of plasma sprayed hydroxyapatite that differed in degree of crystallinity from 15%, 43% to 69%. The interface between the mineralized extracellular matrix and the hydroxyapatite was studied with scanning and transmission electron microscopy. At the onset of bone formation, calcium and phosphorous-rich afibrillar globules, with a diameter varying from 0.2 to 0.8 μm, were produced and deposited onto the different substrata. These globules subsequently fused to form a homogeneous layer to which collagen fibres became anchored. Individual globules could be distinguished on the non-degrading 69 % …

Intravital Microscopic Evidence That Polylactide-Polyglycolide (Plga) Delays Neo-Osteogenesis And Neo-Angiogenesis In Healing Bone, H. Winet, J. O. Hollinger

Cells and Materials

The bone chamber implant (BCI) has allowed monitoring a healing bone defect as well as the effect of an eroding implant on the healing process. The BCI is a useful tool and intravital microscopy a valuable technique for obtaining quantitative data chronicling osseous wound healing. The physiological parameters that form the initial data base documenting healing are neo-osteogenesis and neo-angiogenesis. This review compares and characterizes osseous wound healing in a BCI loaded with an erodible copolymer, PLGA (polylactide-polyglycolide). To determine if a statistically significant deviation from normal healing had occurred, the results were compared with present and historical controls. In …

Simulation Of Daily Precipitation From A Nonparametric Renewal Model, Balaji Rajagopalan, Upmanu Lall, David G. Tarboton

Reports

Wet/dry spell characteristics of daily precipitation are of interest for a number of hydrologic applications (e.g., flood forecasting or assessment of erosion potential). Here, we examine issues related to designing an appropriate nonparametric scheme that focuses on spell characteristics for resampling historically daily precipitation data. A subset of the nonparametric wet/dry spell model presented in Lall et al (1993) is tested with synthetic data to justify the strategy proposed for applications. An application of the nonparametric wet/dry spell model to a Utah data set follows. Performance is judged on a set of statistical measures. Numerical comparisons of model performance with …

A Kernel Quantile Function Estimator For Flood Frequency Analysis, Young-Ii Moon, Upmanu Lall

Reports

A kernel estimator (KQ) of the quantile function is presented here. Boundary kernels are used for extrapolation of tail quantiles. The bandwidth of the estimator is chosen using an automatic, "plus-in" method. Confidence intervals for the estimated quantile are estimated by bootstrapping. Comparisons of the estimator with selected tail probability estimators are offered. The KQ estimator presented here is shown to be competitive with other estimators.

Effectiveness Determination Of Two Different Water-Saving Devices For Toilet Tanks, C. Earl Israelsen, Gilberto Urroz

Reports

Introduction: The project was successful in obtaining eight different brands of new toilet tanks in the 3.5 gals-per-flish-bariety, and two used ones requiring 5.0 gals per flish. The tanks were installed on a t atime on a padded stell frame and connected to a water supply. The float was adjusted to maintain the water in the tank at the level recommended by the manufacturer. When the flush and shut-off mechanisms were adjusted properly, the testing begain.

A Prototype Computer Based Tutorial For The Use Of Highway Patrol Radar Guns, William J. Grenney, Thad E. Senti

Reports

Executive Summary: The software contained in this package, termed POSAT (Police Officer Standards and Training) is the prototype knowledge-base decision support system developed specifically for the Utah State Police Academy. All police officers must be certified annual for the use of radar guns. This self-study computer program is intended to reduce the cost of providing field training for hundreds of police officers each year. By installing the POSAT software at remote locations, polic officers within a certain districut need only to travel to the nearest remote testing sight to take the exam and renew their radar certification. POSAT automatically transmits …

Computer Methodology For Transportation Agencies To Screen Technologies For Hazardous Waste Remediation, William J. Grenney, Ravi Kumar Penmetsa

Reports

Executive Summary: Many pathways exist for transportation agencies and other public and private agencies to become responsible for sites contaminated by hazardous wastes. As a reult, agencies frequently face a multitude of lega, regulatory, financial, technical, and health risk problems. When transportation agencies become involved in the remediation of hazardous waste sites, the common practice is to hire consultants and contractors for the clean up process. Because the field of hazardous waste site remediation is changing so rapidly, agency personnel evaluating the consultant's recommendations need to ahve access to the most recent regulatory and remediation information. Early stages of the …

Development Of Mountain Climate Generator And Snowpack Model For Erosion Predictions In The Western United States Using Wepp: Phase Iv, David S. Bowles, U. Lall, D. G. Tarboton, E. Malek, B. Rajagopalan, T. Chowdhury, E. Kluzak

Reports

Executive Summary: Introduction: This report summarizes work conducted during the funding period (December 1, 1991 through September 30, 1992) of a Research Joint Venture Agreement between the Intermountain Research Station, Forest Service, U. S. Department of Agriculture and the Utah Water Research Laboratory (UWRL), Utah State University (USU). The purpose of the agreement is to develop a Western Mountain Cilmate Generator (MCLIGEN) similar in function to the existing (non-orographic area) Climate Generator (CLIGEN), which is part of the Water Erosion Prediciton Project (WEPP) procedure. Aso, we are developing a Western U.S. Snowpack Simulation Model for includsion in WEPP. In the …

Interpretation Of Drill Log Data: Dlog3d - A Probabilistic Tool For Analyzing Subsurface Soil Variability, Alaa El-Din Ali, Upmanu Lall

Reports

Groundwater contamination potential is directly related to the location of zones of high hydraulic conductivity. Sedimentary depositional environments can be quite complex. Pockets of sand of varying thickness and orientation may be scattered over the aquifer. A contaminant pathway can esist where these pockets or lenses are interconnected. Aquifer pump tests do not provide useful information on the occurrence of such lenses. Drill log data directly sample the soil, but may represent very local sections of the aquifer, and are of relatively poor quality. Here we present a methodology (DLOG3D) that allows a probabilistic interpretation of drill log data that …

Candi (Chemical And Irrigation Management) Users Manual, Vs 2.0., A. H. Aly, Richard C. Peralta

Civil and Environmental Engineering Faculty Publications

This manual describes an interactive computer model, CANOl. CANOl is a decision support tool designed to aid in on-farm pesticides and irrigation management. CANOl simulates downward chemical movement through the soil in response to different irrigation practices. CANOl estimates the relative amount of a non-polar organic chemical which remains in the soil profile by the time it reaches a specified depth. Future releases of CANOl will involve the management of different types of chemicals. CANOl is also able to delineate the wellhead protection areas for domestic wells. For this purpose, CANOl involves USEPA's model (MWCAP). Different versions of CANOl handle …

Best Management Of Pesticide And Irrigation Application Systems: Background And Candi Software, Alaa H. Aly, Richard C. Peralta, Howard M. Deer

Civil and Environmental Engineering Faculty Publications

In this chapter, we discuss a commonly-used volume balance approach for simulating the movement of water through the root zone. We then show how this approach can be coupled with pesticide leaching prediction. Finally we show how information about irrigation system design can be used with the previous processes. The result is an integrated approach for estimating the environmental consequences of irrigation and pesticide management.

Ground-Water Policy-Making Support: Usem Optimization Modelling Plus Gis And Graphics, Richard C. Peralta, Christopher M. U. Neale, Ali Gharbi, Mazibur Khan, Oscar Daza, Douglas Ramsey, Kurt Vest

Civil and Environmental Engineering Faculty Publications

A support tool for ground-water management decision making involves integration of a multiobjective optimization model, GIS and graphics software. Two results are improved consideration of spatial data within the optimization model and improved conversion of optimal strategies to real-world application. Another result is improved visualization of the trade-offs involved between conflicting management goals.

Us/Wells Vs. 1.05 User's Manual, Alaa H. Aly, Richard C. Peralta

Civil and Environmental Engineering Faculty Publications

Presented is a computer program, US/WELLS, that is designed to solve several types of groundwater management problems. The acronym US/WELLS stands for Utah State WELL System. This decision-support tool is usable by persons slightly familiar with groundwater hydraulics. It can be valuable for practical management of groundwater systems that satisfy certain criteria.

Sprinkler Irrigation-Pesticide Best Management Systems, A. Y. Ranjha, Richard C. Peralta, Robert W. Hill, A. M. Requena, Howard M. Deer, M. Ehteshami

Civil and Environmental Engineering Faculty Publications

The relative reduction in potential groundwater contamination due to pesticides at several sites in Utah was determined by comparing alternative irrigation system designs, water management practices, and pesticides. Alternative sprinkler irrigation distribution coefficients were used to estimate irrigation application depths. The movement of pesticides through soils following sprinkler irrigations was simulated with a one-dimensional model. Pesticide contamination of groundwater can be reduced by careful selection of pesticides, using properly designed irrigation systems, and improved water management techniques. Procedures for selecting an appropriate sprinkler system design and pesticide are presented. KEYWORDS. Pesticides, Leaching, Sprinkler irrigation, Irrigation depth, Irrigation system design, Soil, …

Comparison Of Optimization Methodologies For Sustained-Yield Groundwater Pumping Planning In East Shore Area, Utah, Shu Takahashi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Combined simulation and optimization models, which are helpful for long-term groundwater planning of complex nonlinear aquifer systems, are developed using alternative modelling approaches. The models incorporate a representation of steady-state, quasi-three-dimensional head response to pumping within an optimization. An embedding model which describes exactly the nonlinear flow of an unconfined aquifer is presented. In contrast with the embedding models presented in the Utah State University Ground Water Model, it directly achieves the optimal solution without a "cycling." To address the nonlinearity of the flow system, response matrix models couple superposition with the cycling procedure. Their linear influence coefficients are generated …

Microstructure Of Mica Glass-Ceramics And Interface Reactions Between Mica Glass-Ceramics And Bone, W. Holand, W. Gotz, G. Carl, W. Vogel

Cells and Materials

This review paper characterizes glass-ceramics containing mica as main crystal phase. The phase formation reactions in dependence of the chemical composition and the microstructure are shown. Microstructure of mica glass-ceramics has been studied by electron replica and scanning electron microscopic (SEM) techniques.

Mica glass-ceramics have previously been developed in Si02-B20rA120rMgO-F--base glasses. The material is machinable because of the precipitation of micas of fluorophlogopite-type. Also, a machinable glass-ceramic for dental applications was developed based on KMg2_5(Si40 10)F2-micas. We developed mica glass-ceramics in the Si02-Al20rMgO-NaiO-K20-F glass system. Phase formation within these glasses was observed by SEM. A double controlled nucleation and crystallization …

The Effect Of Polymethylmethacrylate Bone Cement Vibration On The Bone-Cement Interface, A. M. C. Thomas, D. J. W. Mcminn, M. Haddaway, I. W. Mccall

Cells and Materials

Low frequency vibration of polymethylmethacrylate (PMMA) bone cement reduces the viscosity of the cement by shear thinning. The effect of this low frequency vibration on the bone-cement interface was studied using microfocal radiography (MFR) and scanning electron microscopy (SEM). Effects were studied in-vitro and in-vivo. In-vitro, samples of Palacos low viscosity PMMA were placed on blocks of Kiel bone and vibrated. MFR and SEM demonstrated an improvement in the appearance of the bone-cement interface. In-vivo, PMMA was injected into the upper tibia of the dog. An assessment of the effect of high and low pressure injection, and the effect of …

Ultrastructural Assessment Of Lesion Development In The Collared Rabbit Carotid Artery Model, J. E. Beesley, A. C. Honey, J. F. Martin

Cells and Materials

Cellular reactions associated with the formation of lesions generated in the carotid artery of rabbits fed either normal or high cholesterol diets by the placement of a flexible, silastic collar around the artery, were studied by electron microscopy.

Endothelial cells remained as a monolayer throughout lesion development. The endothelial cell surface in both experimental and sham operated carotids, 4 hours and 8 hours after the initiation of the experiments, were covered with platelets and leukocytes. Neutrophils were present until 7 days in the arteries from within the collar of animals maintained on a normal diet, but only to 1 day …

The Effect Of Serial Passaging Of Lactobacilli In Liquid Medium On Their Physico-Chemical And Structural Surface Characteristics, Pieter L. Cuperus, Henny C. Van Der Mei, Gregor Reid, Andrew W. Bruce, Antoine E. Khoury, Paul G. Rouxhet, Henk J. Busscher

Cells and Materials

The aim of this study was to examine the effect of serial passaging in liquid medium on physico-chemical and structural surface characteristics of two lactobacillus strains. To this end, starting cultures (p = 1) of urogenital isolate L. acidophilus RC 14 and poultry isolate L. fermentum B54 were serially passaged in liquid medium up to 20 times (p = 20). In both strains, similar physico-chemical and structural changes were observed following serial passaging. According to water contact angle measurements , cells in p = 20 cultures became less hydrophobic. Microelectrophoresis showed that isoelectric points shifted towards the acidic region upon …

The Incorporation Of Glass-Ceramic Implants In Bone After Surface Conditioning Glow-Discharge Treatment, C. M. Muller-Mai, C. Voigt, R. E. Baier, U. M. Gross

Cells and Materials

Glow discharge (GD)-treated and autoclaved glass-ceramics of bone-bonding and non-bonding type were implanted into the femoral diaphysis of rats for 3, 7, 14 and 28 days and were investigated by applying light microscopy and histomorphometry , and scanning and transmission electron microscopy. More bone and chondroid, and faster osteoid development were observed at glow-discharge treated implants even in nonbonding implants when compared to autoclaved controls. Ultrastructural investigations showed a higher leach ing- rate of GD-treated bone-bonding implants during the early days after implantation leading to a higher rugosity, whereas no morphological changes were observed on non-bonding implant surfaces. More extracellular …

Applications Of Fourier Transform Infrared (Ft-Ir) Microscopy To The Study Of Mineralization In Bone And Cartilage, Adele L. Boskey, Nancy Pleshko, Stephen B. Doty, Richard Mendelsohn

Cells and Materials

Knowledge of the phase, composition, and crystallite size and perfection of the mineral in normal and abnormally calcified tissues provides insight into the mechanism by which this mineral was deposited. These data also can be used to develop rational therapies for pathological conditions characterized by abnormal mineral deposition. As illustrated in this review, coupling of an optical microscope with a Fourier transform infrared (FT-IR) spectrophotometer permits the mapping at 20 μm spatial resolution of changes in mineral characteristics (content, particle size, composition) in the growth plate, in bone biopsies, in mineralizing cell culture systems, and in soft tissue calcifications. Based …

A Model For A Model: Pressure Jackets On Nude Mice With Implants Of Hypertrophic Scars, C. Ward Kischer, Jana Pindur

Cells and Materials

The hypertrophic scar, a consequence of deep surface injury, is peculiar to humankind. Subcutaneous implants of these tissues in nude mice provide a quasi - animal model for experimental use. An efficacious treatment to promote acceleration of maturation of the hypertrophic scar is controlled topical pressure produced by flexible dacron fabric wraps. Implants in the nude mouse provide a model for study of the effects of pressure. Preliminary results indicate that those implants in nude mice, responding favorably to pressure jackets, show changes consistent with those previously obtained in human studies. The collagen matrix shows reduced density and parallel layering …

Quantitative Scanning Electron Microscopy (Sem) To Study The Adhesion And Spreading Of Human Endothelial Cells To Surface-Modified Poly(Carbonate Urethane)S, C. James Kirkpatrick, Tillmann Otterbach, Dirk Anderheiden, Johannes Schiefer, Horst Richter, Hartwig Hartwig Hocker, Christian Mittermayer, Albert Dekker

Cells and Materials

The polymers currently in use as vascular prostheses are, in the native state, poor substrates for endothelial cell (EC) adhesion and growth. This has a negative effect on the success of pre-seeding regimes. One attempt to improve the polymer substrate is to covalently couple reactive molecules to the surface, which can be used as anchorage points for EC or serve as spacer molecule to couple biological signal molecules such as oligopeptides. We have used a digitized image analysis system coupled to a scanning electron microscope to study the adhesion and spreading of human venous EC to unmodified poly(carbonate urethane) (uPCU), …

Comparative Assessment Of The Cytotoxicity Of Various Substrates In Organ Culture And Cell Culture: A Scanning Electron Microscopy Study, J. L. Duval, R. Warocquier-Clerout, M. F. Sigot-Luizard

Cells and Materials

A comparative study of the behavior of chick embryo endothelial cells grown on various substrates was performed in order to establish the reliability and the limitation of both cell and organ culture methods. Following substrates were analyzed to compare these two different culture techniques: bovine serum albumin, pigskin gelatin and albumin + gelatin cross-linked by glutaraldehyde or carbodiimide, fibrin glue and negative control (Thermanox*). Parameters of cell growth and adhesion were calculated and compared with electron microscopic observations of cell morphology and of the extracellular matrix. Both culture methods provided complementary results and led to a similar classification of the …

Scanning Electron Microscopy Of The Bone Interface With Titanium, Titanium Alloy And Hydroxyapatite, R. D. Orr, J. D. De Bruijn, J. E. Davies

Cells and Materials

Screw implants of commercially pure titanium and titanium alloy together with rods of slip-cast dense hydroxyapatite were implanted transfemorally in young adult rats. The femora were harvested after fixation and freeze-fractured in liquid nitrogen to create two tissue fragments of each specimen. The first contained the implant while the second enabled examination of the tissue immediately adjacent to the implant. In all cases, the tissue abutting the implant was a cement line-like layer which separated the implant from the surrounding bone proper. Greater adherence of this interfacial tissue was demonstrated to hydroxyapatite samples compared with the titanium screws, while no …

Microstructural Changes In Retrieved Amalgam Restorations, Grayson W. Marshall Jr., D. D. Mccoy, John H. Kinney, Hugo Letzel, Sally J. Marshall

Cells and Materials

Many blended or dispersed phase high Cu amalgams show excellent clinical performance in long term clinical trials. However, little is known concerning the microstructural changes that occur during clinical use of these systems. This investigation sought to characterize some of the changes that occur in the Ag-Cu particles and their surrounding reaction zones based on a study by backscattered scanning electron microscopy (BSEM), energy dispersive spectroscopy and a new rnicroprobe technique called Colorad which assigns various colors to elements and element combinations and indicates changes in elemental concentration by changes in color value (brightness). Restorations were retrieved from clinical trials …

The Role Of Implant Surface Geometry On Mineralization In Vivo And In Vitro; A Transmission And Scanning Electron Microscopic Study, B. Chehroudi, J. Ratkay, D. M. Brunette

Cells and Materials

The purpose of th.is study was to examine the effect of substratum surface topography on bone formation in vivo and in vitro. Precise control over substratum topography was achieved using micromachining, a technique developed from the fabrication of microelectronic components. In the in vivo studies, titanium-coated epoxy replicas of micromachined surfaces were implanted subcutaneously in the parietal area of rats. After 6 weeks, bone-like tissue was found adjacent to some micromachined surfaces. Detailed observation of this tissue with the transmission electron microscope revealed osteoblast/osteocyte-like cells and a fully or partially mineralized collagenous matrix. Mineralized matrix and collagen bundles were …

Bone Regeneration Materials For The Mandibular And Craniofacial Complex, Jeffrey Hollinger, Anshumali Chaudhari

Cells and Materials

Due to different functional demands and vascularity, the mandibular and craniofacial complex require bone regenerating materials with physical and chemical properties that are different from long bone. However, certain common requirements to both locales must be addressed. Furthermore, reasons why the autograft and allogeneic bank bones are successful in regenerating bone need to be understood if the researcher is to develop satisfactory alternatives. The purpose of this paper is to review some of the requirements needed for bone regenerating materials for mandibular and craniofacial bone repair. In that effort, we have briefly described the autograft and allogeneic bank bones, animal …