Engineering Commons^™

Biomechanical Properties Of Hemlocks: A Novel Approach To Evaluating Physical Barriers Of The Plant-Insect Interface And Resistance To A Phloem-Feeding Herbivore, Paul Ayayee, Fuqian Yang, Lynne K. Rieske

Entomology Faculty Publications

Micromechanical properties that help mediate herbivore access may be particularly important when considering herbivorous insects that feed with piercing-sucking stylets. We used microindentation to quantify the micromechanical properties of hemlock, Tsuga spp., to quantify the hardness of the feeding site of the invasive hemlock woolly adelgid, Adelges tsugae. We measured hardness of the hemlock leaf cushion, the stylet insertion point of the adelgid, across four seasons in a 1 y period for four hemlock species growing in a common garden, including eastern, western, mountain, and northern Japanese hemlocks. Leaf cushion hardness was highest in the fall and winter and …

Developent Of A Phospholipid Encapsulation Process For Quantum Dots To Be Used In Biologic Applications, Logan Grimes

Master's Theses

The American Cancer Society predicts that 1,665,540 people will be diagnosed with cancer, and 585,720 people will die from cancer in 2014. One of the most common types of cancer in the United States is skin cancer. Melanoma alone is predicted to account for 10,000 of the cancer related deaths in 2014. As a highly mobile and aggressive form of cancer, melanoma is difficult to fight once it has metastasized through the body. Early detection in such varieties of cancer is critical in improving survival rates in afflicted patients. Present methods of detection rely on visual examination of suspicious regions …

Ultraviolet Led Biofouling Mitigation, Andrew Lam

Computer Engineering

The goal is to determine if low-cost UV LEDs can mitigate marine biofouling on small glass or acrylic camera lenses. A microprocessor-controlled experimental setup to control the illumination of low-cost UV LEDs of various wavelength and packaging was fashioned. The system consists of a programmed microcontroller, a manufactured LED breakout interface, and a submergible UV LED array enclosed in a borosilicate glass tube. A preliminary qualitative assessment of four different UV LEDs was conducted during a three-week deployment of the experimental setup in the raw seawater system at the Cal Poly Center for Costal and Marine Sciences in Avila Beach, …

Team Joseph: Adaptive Aquatic Device, Paul Sands, Lilly Hoff, Andrea Voigt

Mechanical Engineering

The scope of this project included designing and fabricating an adaptive aquatic device for Joseph, a 20 year old student in the Special Education Program at San Luis Obispo High School with a subset of cerebral palsy known as spastic quadriplegia. The project was presented at the beginning of the Fall 2013 quarter to the mechanical engineering students at Cal Poly with the aspiration that a team of engineers would construct a device that would allow Joseph, his friends and family to compete in their first triathlon on July 27, 2014. The project was humbly accepted by mechanical engineering students …

Smooth Muscle Cell Organization In The Stem Region Of The Gracilis Collateral Circulation, Amanda Krall

Biomedical Engineering

Many patients who suffer from the ischemic Peripheral Arterial Occlusive Disease (PAOD) experience intermittent claudication, which can be attributed to impaired vasodilation. Collateral vessels are the primary site of resistance to blood flow downstream; therefore maximizing vasodilation in collaterals is crucial for efficient circulation. Collaterals function as natural bypasses around the occluded arteries and the increase in flow into these vessels causes them to outwardly remodel into conduit vessels. However, functional vasodilation in the stem region of collateral vessels is impaired at day 7 following femoral ligation, which can be attributed to smooth muscle cell malfunction. However, the increase in …

Optimization Of Growth Parameters For Algal Regrowth Potential Experiments, Chadwick D. Boggess

Master's Theses

Water cost is one of the key deterrents in making algal biofuels a feasible alternative to traditional petroleum fuels. Research into recycling of algal media was conducted in a laboratory setting at the California Polytechnic State University in San Luis Obispo. A growth assay was created to allow for algal cultures, specifically Ward’s Chlorella sp., to proliferate in sealed culture vessels. Bold’s Basal Medium (BBM) was selected as the media due to its extensive use with freshwater algal strains. However, this medium can limit algal growth through nutrient limitation and it became necessary to augment the original recipe. 50mM …

Project Proposal And Development Plan For A New Security Gate At Swanton Pacific Ranch, K. Morgan Perry

Agricultural Education and Communication

The purpose of this project was to develop a prototype gate for Cal Poly’s Swanton Pacific Ranch in Santa Cruz, CA. Swanton Pacific Ranch requested that the author design and build a 21’x5’ single-retractable gate for Swanton Pacific Ranch to help decrease the security issues that have recently arisen at the facility. The design and format of this project are focused towards the desires and goals set forth by Swanton Pacific Ranch and the Operations Manager, Steve Auten in order to obtain a certain outcome. This project provides an outline of what needs to be done in order to produce …

Adaptive Basketball Shooter - Final Project Report, Jenna N. Becker, Richard A. Galanti, Justin D. Haley, Brett Y. Nayudu

Mechanical Engineering

The Friday Club is a joint venture between the Cal Poly Kinesiology Department and the San Luis Obispo Special Olympics that offers people with varying degrees of disability the opportunity to meet weekly and learn various sports and games. At Friday Club, athletes in wheelchairs with limited arm strength use devices built by Cal Poly mechanical engineering students in order to participate in various sports. Many devices are designed to attach to the Universal Play Frame (UPF), a wheel-chair attachment. The purpose of this project was to design and build a UPF device that will launch a basketball, so that …

Is Biofuel A Feasible Long-Term Chief Energy Source? A Global Perspective, Rajee Olaganathan, Aston Lee, Debra Tong, Mameegate Zheng Jun Cheston, Zack Ho Xuan Yi

Publications

This scientific paper examines the feasibility of officiating biofuels as the future chief energy source of the world. It weighs the pros and cons of the four different generations of biofuels that have been discovered over time, explaining how the raw materials used in each generation determines each individual level of long-term sustainability. It then further analyses both general advantages and disadvantages, comprising of economic, social, health and environmental impacts; giving the reader an unbiased perspective. Few case studies have also been reviewed to better understand the implications of drawing energy from biofuels, as well as the current state of …

The Santa Clara, 2014-05-22, Santa Clara University

The Santa Clara

No abstract provided.

The Santa Clara, 2014-05-15, Santa Clara University

The Santa Clara

No abstract provided.

Evaluation Of Methods For Control Of Vegetation In Utility Corridors, George V. Rancea

Master's Projects and Capstones

Vegetation management programs are now one of the largest budget items for most of the utility companies in the United States. Control methods used in line clearance activities include mechanical trimming, planting management, or use of tree growth regulators (chemicals that suppress the production of gibberellins, the plant hormones that control stem elongation). Choosing the appropriate control method is a decision that vegetation program managers should take based on many factors, like costs, environmental impacts, existing agreements with the landowners, terrain, public perception etc. To find a balance between competing interests – a reliable and economic electric system on one …

Determining Forage Intake Of Nursing Calves Grazing Native Sandhills Rangeland, Jennifer A. Walker

Department of Animal Science: Dissertations, Theses, and Student Research

Effective management of grazing lands is important for longevity and productivity. Accurate calculations of stocking rates per animal unit will aid in long term sustainability. Calculating stocking rates for cattle can prove challenging for cows and nursing calves. Limited research has been conducted to determine forage intake of the nursing calf pre-weaning. The objective of Trial 1 was to determine nursing calf forage intake over time until weaning. Trial 1 used 8 crossbred cow-calf pairs in experiment 1 and 12 pairs in experiment 2. Pairs were separated into two groups rotating between grazing upland range or housed in individual pens …

The Santa Clara, 2014-05-08, Santa Clara University

The Santa Clara

No abstract provided.

The Santa Clara, 2014-05-01, Santa Clara University

The Santa Clara

No abstract provided.

Investigating Antibiotic Resistance Levels Of Salmonella Internalized In Lettuce Leaves, Jason B. Thomas

McNair Scholars Research Journal

Contamination of food crops by the human pathogen Salmonella is a food safety threat worldwide. Though using treated wastewater for irrigation is a sustainable practice, it may introduce trace levels of Salmonella that may contaminate food crops. Salmonella could develop resistance to antibiotics present in wastewater. The overall goal of the project is to increase the understanding of the public health risk associated with the use of treated wastewater to irrigate food crops. The objective of this particular study is to determine the antibiotic resistance level of Salmonella internalized in lettuce leaves. In this experiment, thirty-six plants of the lettuce …

Modeling The Adaptive Immune Response To Mutation-Generated Antigens, Rory J. Geyer

University Scholar Projects

Somatic mutations may drive tumorigenesis or lead to new, immunogenic epitopes (neoantigens). The immune system is thought to represses neoplastic growths through the recognition of neoantigens presented only by tumor cells. To study mutations as well as the immune response to mutation-generated antigens, we have created a conditional knockin mouse line with a gene encoding, 5’ to 3’, yellow fluorescent protein (YFP), ovalbumin (which is processed to the immunologically recognizable peptide, SIINFEKL), and cyan fluorescent protein (CFP), or, YFP-ovalbumin-CFP. A frame shift mutation has been created at the 5’ end of the ovalbumin gene, hence YFP should always be expressed, …

In Vivo Evaluation Of An Automated Pressure Sore Reducer, Linda J. Cross

Honors Capstones

No abstract provided.

Computational Modeling Of Drug Release From Bone Scaffolds, Corey Landry

Honors Capstones

No abstract provided.

Starch-Pectin Matrices For Encapsulation Of Ascorbic Acid, Yiwei Liu

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Starch and pectin are two food-grade carbohydrates widely utilized in the food industry. Starch and pectin polymers have been investigated in encapsulating functional food ingredients and in pharmaceutical applications. Resistance to enzyme hydrolysis, differential solubilities, depending on the pH, and the ability to ‘protect’ unstable molecules are considered some of the beneficial properties of starch and pectin polymers, for encapsulation applications. Food ingredients could be delivered in a controlled manner to a specific target by encapsulating in micro-scale particles, i. e., microencapsulation. Two studies were conducted to investigate the ability of selected starch-pectin blends in microencapsulating ascorbic acid (vitamin C) …

Response Of Large River Fishes To A Prolonged High Water Event In The Missouri River, Nebraska, Nicholas Paul Hogberg

School of Natural Resources: Dissertations, Theses, and Student Research

Regulation and modification of large rivers to accommodate human uses have been a root cause of freshwater biodiversity declines. The Missouri River is among the most drastically-altered large river systems in North America, with a series of mainstem impoundments in the upper watershed altering flow characteristics downstream, and channelization throughout the lower river homogenizing instream habitat and reducing off-channel habitat. Precipitation events during the winter and spring 2010-2011 caused flooding of the greatest magnitude and duration since reservoir completion. The large magnitude and long duration of this flood made it unlike any flood in recent history and provided a unique …

Tht And Capillary Electrophoresis To Monitor The Effects Of Solutions Conditions On Amylin Aggregation, Michael May

Graduate Theses and Dissertations

Amylin (hIAPP) aggregates have been found in 90% of patients with type II diabetes at autopsy, and are suspected to play a role in the death of islet &beta-cells¹. However, this aggregation process is not well understood. Here, we explore methods that utilize capillary electrophoresis (CE) as a means to better understand amylin's aggregation process.

We examined the effects of solutions conditions: agitation, pH, salt, and temperature on amylin aggregation using Thioflavin T, dot blots, and capillary electrophoresis. Thiofalvin T was used to predict the lag time to &beta-sheet formation. Our results indicated all variables with the exception …

Biomechanical Analysis Of Gait Kinetics Resulting From Use Of A Vacuum Socket On A Transtibial Prosthesis, Maria De Lourdes Ramos Gonzalez

Honors College Theses

The technology and design of lower limb prosthetics have evolved greatly since their introduction. The current study proposed to compare the effects of a conventional pin socket attachment and a vacuum socket attachment for a transtibial amputee. Whereas traditional measurements of gait utilize force plates and camera systems, this study made use of a small tri-axial load cell located at the base of the socket to measure the forces and moments exerted during a regular gait cycle. The hypothesis tested stated that a vacuum pump socket attachment, when compared to a non-vacuum (pin) socket, will have a significant effect on …

Modeling The Adaptive Immune Response To Mutation-Generated Antigens, Rory J. Geyer

Honors Scholar Theses

Somatic mutations may drive tumorigenesis or lead to new, immunogenic epitopes (neoantigens). The immune system is thought to represses neoplastic growths through the recognition of neoantigens presented only by tumor cells. To study mutations as well as the immune response to mutation-generated antigens, we have created a conditional knockin mouse line with a gene encoding, 5’ to 3’, yellow fluorescent protein (YFP), ovalbumin (which is processed to the immunologically recognizable peptide, SIINFEKL), and cyan fluorescent protein (CFP), or, YFP-ovalbumin-CFP. A frame shift mutation has been created at the 5’ end of the ovalbumin gene, hence YFP should always be expressed, …

Cryoprotectant Loading In A Microchannel, Shelby Pursley

Honors Capstones

No abstract provided.

Comparing Virus Ultrafiltration Of Bacteriophages With Filtration Of Minute Virus Of Mice, Kent Smith

Graduate Theses and Dissertations

Industrial production of protein therapeutics demand rigorous testing and clearance of viruses. The U.S. Food and Drug Administration dictate the purity of pharmaceuticals with regards to viral contamination. As this testing is time consuming and expensive using mammalian cells and viruses, bacteriophages may provide a faster and cheaper alternative for membrane filtration processes. We used ultrafiltration membranes to filter protein solutions with viruses. Two bacteriophages were tested against membranes with two different pore sizes. These membranes were then tested by inverting the membrane's orientation. Flux measurements and log virus removal data were taken. Flux and log virus removal were seen …

Identifying, Monitoring, Quantifying And Converting Algae To Bio-Fuels In Bio-Reactors, Alice C. Jernigan

Graduate Theses and Dissertations

Growing algae as a source for bio-fuels has become an area of interest due to concerns about global warming and the reliability and ecology of the production of fossil fuels. Dried algae harvested from a pilot water quality improvement technology at the Rockaway Wastewater Treatment Facility in New York were examined as a source of carbohydrates and lipids for the production of bio-fuels in bio-reactors. The length of storage time, storage conditions, sugar and lipid extraction processes, and fuel production were studied. The results show that if the algae is stored dry (0.015 g/g algae even after a year in …

Asymmetric Unilateral Transfemoral Prosthetic Simulator, Tyagi Ramakrishnan

USF Tampa Graduate Theses and Dissertations

amputation, which includes reduced force generation at the knee and ankle, reduced control of the leg, and different mass properties relative to their intact leg. The physical change in the prosthetic leg leads to gait asymmetries that include spatial, temporal, or force differences. This altered gait can lead to an increase in energy consumption and pain due to compensating forces and torques. The asymmetric prosthesis demonstrated in this research aims to find a balance between the different types of asymmetries to provide a gait that is more symmetric and to make it overall easier for an amputee to walk.

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Progress Of Mesenchymal Stem Cell Therapy For Neural And Retinal Diseases, Tsz Kin Ng, Veronica R. Fortino, Daniel Pelaez, Herman S. Cheung

Biology Faculty Articles

Complex circuitry and limited regenerative power make central nervous system (CNS) disorders the most challenging and difficult for functional repair. With elusive disease mechanisms, traditional surgical and medical interventions merely slow down the progression of the neurodegenerative diseases. However, the number of neurons still diminishes in many patients. Recently, stem cell therapy has been proposed as a viable option. Mesenchymal stem cells (MSCs), a widely-studied human adult stem cell population, have been discovered for more than 20 years. MSCs have been found all over the body and can be conveniently obtained from different accessible tissues: bone marrow, blood, and adipose …

The Santa Clara, 2014-04-24, Santa Clara University

The Santa Clara

No abstract provided.