Engineering Commons^™

Aqueous Reactive Species Induced By A Surface Air Discharge: Heterogenous Mass Transfer And Liquid Chemistry Pathways, D. X. Liu, Z. C. Liu, C. Chen, A. J. Yang, D. Li, M. Z. Rong, H. L. Chen, M. G. Kong

Bioelectrics Publications

Plasma-liquid interaction is a critical area of plasma science and a knowledge bottleneck for many promising applications. In this paper, the interaction between a surface air discharge and its downstream sample of deionized water is studied with a system-level computational model, which has previously reached good agreement with experimental results. Our computational results reveal that the plasma-induced aqueous species are mainly H+, nitrate, nitrite, H2O2 and O-3. In addition, various short-lived aqueous species are also induced, regardless whether they are generated in the gas phase first. The production/loss pathways for aqueous species are quantified for an air gap width ranging …

Incorporating Machine Vision In Precision Dairy Farming Technologies, Anthony N. Shelley

Theses and Dissertations--Electrical and Computer Engineering

The inclusion of precision dairy farming technologies in dairy operations is an area of increasing research and industry direction. Machine vision based systems are suitable for the dairy environment as they do not inhibit workflow, are capable of continuous operation, and can be fully automated. The research of this dissertation developed and tested 3 machine vision based precision dairy farming technologies tailored to the latest generation of RGB+D cameras. The first system focused on testing various imaging approaches for the potential use of machine vision for automated dairy cow feed intake monitoring. The second system focused on monitoring the gradual …

Monitoring Yogurt Culture Fermentation And Predicting Fermentation Endpoint With Fluorescence Spectroscopy, Timothey P. Mains, Frederick Alan Payne, Michael P. Sama

Biosystems and Agricultural Engineering Faculty Publications

Determination of the endpoint of yogurt culture fermentation is a process parameter that could benefit from automation. The feasibility of using a fluorescence sensor technology based on 280 nm excitation and 350 nm emission to predict the endpoint of yogurt culture fermentation was investigated and compared with the endpoint prediction from a near-infrared (880 nm) light backscatter sensor. Yogurt cultures with three levels of milk solids (8%, 10%, and 12%) and three temperatures (40°C, 43°C, and 46°C) were tested with three replications in a 3 x 3 factorial design (n = 27). Prediction models were developed for each optical measurement …

Effects Of Chemical Amendments To Swine Manure On Runoff Quality, Elizabeth L. Bullock, Dwayne R. Edwards, Phillip A. Moore Jr., Richard S. Gates

Biosystems and Agricultural Engineering Faculty Publications

Land-applied swine manure can be an environmental concern when runoff losses of manure constituents occur. The use of chemical amendments to mitigate these losses has been investigated for poultry litter, but materials such as swine manure have received less attention in this context, particularly at the plot scale or larger. The objective of this experiment was to evaluate the impacts of aluminum sulfate (alum; Al₂(SO₄)₃), aluminum chloride (AlCl₃), and ferric chloride (FeCl₃) addition on runoff of selected constituents of land-applied swine manure. Manure was collected from feeder pigs fed a …

Anticancer, Biophysical And Computational Investigations Of Half-Sandwich Ruthenium(Ii) Thiosemicarbazone Complexes: The Effect Of Arene Versus Thiacrown Face-Cap, Floyd A. Beckford, Alyssa Stott, P. Canisius Mbarushimana, Marc-Andre Leblanc, Kinsey Hall, Samantha Smith, Jimmie L. Bullock, Dennis J. Houghton, Alvin A. Holder, Nikolay Gerasimchuk, Antonio Gonzalez-Sarrías

Chemistry & Biochemistry Faculty Publications

A series of half-sandwich ruthenium complexes, two containing an arene face-cap and the other a thiacrown ether face-cap were synthesized to investigate the necessity of the arene for anticancer activity in this class of compounds. The complexes are formulated as [(h⁶-p-cymene)Ru(dmabTSC)Cl]PF₆, [(h⁶-benzene)Ru(dmabTSC)Cl]PF₆ (arene complexes), and [([9]aneS₃(dmabTSC)Cl]PF₆ (dmabTSC = dimethylaminobenzaldehye thiosemicarbazone). It was observed that none of the complexes showed good anticancer activity in vitro against HCT-116 and Caco-2 (colon adenocarcinoma) cells. All three complexes can bind strongly to calf-thymus DNA with binding constants on the order of 10 …

Assessing Public Health Burden Associated With Exposure To Ambient Black Carbon In The United States, Ying Li, Daven K. Henze, Darby Jack, Barron H. Henderson, Patrick L. Kinney

ETSU Faculty Works

Black carbon (BC) is a significant component of fine particulate matter (PM_2.5) air pollution, which has been linked to a series of adverse health effects, in particular premature mortality. Recent scientific research indicates that BC also plays an important role in climate change. Therefore, controlling black carbon emissions provides an opportunity for a double dividend. This study quantifies the national burden of mortality and morbidity attributable to exposure to ambient BC in the United States (US). We use GEOS–Chem, a global 3-D model of atmospheric composition to estimate the 2010 annual average BC levels at 0.5 x 0.667° …

Using Zeolite For Soil Moisture Retention And Salinity Control, Mark Torres

Open Access Theses & Dissertations

There is a great need in the desert southwest United States for exploring ways to save water in agriculture as it accounts for up to 90 percent of water consumption in many western states (USDA 2016). As surface water reaches critical lows the dependence on ground water has increased leading to the need for deeper wells with higher salt concentrations. The studies conducted for this research included using zeolites from the St Cloud Mining Company in Winston, New Mexico to determine the feasibility of using these zeolites to help and retain moisture in zeolite treated soils. The Volumetric Water Content …

High Fidelity Localization And Sensing For Cattle Analytics, Luis Carlos Bañuelos Chacon

Open Access Theses & Dissertations

Global beef production is projected to reach approximately 60 million tons in 2015 and opportunities to increase efficiencies are significant with recent advancements in remote sensing. By economically monitoring the location and conditions of a herd such as feeding patterns, body temperature, heart rate, and other biometric information, the herd performance can now be enhanced through data-driven optimization. Next generation sensors provide high fidelity data on a wide range of measurable properties, and simultaneously, the wide adoption of these sensor systems in many consumer applications is leading to commoditization and reduced cost due to the associated economies of scale. Deploying …

Vascularization In Interconnected 3d Printed Ti-6al-4v Foams With Hydrogel Matrix For Biomedical Bone Replacement Implants, Victor Correa

Open Access Theses & Dissertations

Abstract:

Vascularization or angiogenesis on newly implanted metal orthopedic implants has remained a challenge in the field of tissue engineering. In this research, an interconnected foam structure of Ti-6Al-4V was micro-fabricated by Electron Beam Melting (EBM) technique. The foam in question has a density of 1.77g/cm3 with 60% porosity and a tensile strength of 18GPa. An Extracellular Matrix based hydrogel was added as an aqueous matrix to the foam in question. Hypoxia mimetic stress has been closely related to many wound healing biomedical applications as it increases survival and proliferation molecular signals. To that end, increased expression of Hypoxia-Inducible Factor-1α …

Development And Initial Testing Of A Low-Cost, Electronic, Microprocessor-Controlled Prosthetic Knee, Lucas Jonathan Galey

Open Access Theses & Dissertations

Cost-effective lower-limb prostheses have been successful in restoring mobility, independence, and a way of life to millions of global amputees who do not have the means to afford more sophisticated prosthetics. Comprehensively, the current above knee (AK) prosthetic market is segmented into two extremes â?? very affordable relief style knees that offer basic functionality with high risk of accident due to falls, and very expensive styles that offer electronic microprocessor stumble control and adaptive cadence. There remains a gap for a middle-ground system that provides stumble control and greater stability within an achievable price bracket for the developing world. This …

The Viability Of High-Frequency Oscillation Analysis In Eeg Signals For Seizure Prediction, Bryan David Kern

Open Access Theses & Dissertations

Seizure prediction is a decades-old research problem that has yet to reach any satisfying conclusions. Many studies have tackled the problem with varying results, but there has yet to be any major breakthroughs that define the direction of all future research. However, a promising, new predictor may have presented itself in recent years in the form of high frequency oscillations (HFOs). With the discovery of HFOs (80 - 800 Hz) as a biomarker for epilepsy, new interest has been placed in studying the high frequency content of EEGs to find a possible link between epilepsy and HFOs. In this paper, …

A Flexible Pet-Based Wearable Sensor For Arterial Pulse Waveform Measurement, Dan Wang, Dean Krusienski, Zhili Hao

Mechanical & Aerospace Engineering Faculty Publications

In light of the need of health monitoring, the paper presents a flexible polyethylene terephthalate (PET)-based wearable sensor for arterial pulse waveform measurement. The sensor encompasses a polydimethylsiloxane (PDMS) microstructure embedded with an electrolyte-enabled 5×1 transducer array, which spans 6mm and has a spatial resolution of 1.5mm. A pulse signal exerts a deflection on the microstructure and is recorded as a resistance change by a transducer at the site of the pulse. An untrained individual can easily align the sensor on a targeted artery with a negligible margin and then acquire the arterial pulse waveform continuously and non-invasively. This sensor …

The Role Of E-Cadherin Force In The Maintenance Of Homeostasis In Epithelial Acini, Fnu Vani Narayanan

Theses and Dissertations

Numerous three-dimensional model systems have emerged for emulating the biochemical and physiological states of native tissue. Yet little is known about the effects of mechanical forces on cell behavior in the context of an organized tissue structure in three-dimensional cell-culture. Epithelial cells cultured in a three-dimensional environment comprised of extracellular matrix proteins form spheroids of polarized cells. Cellular responses to mechanical cues, generated from dynamic interactions with the extracellular matrix and neighboring cells, are known to influence cellular behavior to a great extent. Previous studies have shown that tumorigenic progression has been frequently linked to the down regulation of E-cadherin, …

Early Life History Of Three Pelagic-Spawning Minnows Macrhybopsis Spp. In The Lower Missouri River, T. A. Starks, M. L. Miller, J. M. Long

United States Army Corps of Engineers, Omaha District

Life-history characteristics of age-0 sturgeon chub Macrhybopsis gelida, shoal chub Macrhybopsis hyostoma and sicklefin chub Macrhybopsis meeki were compared using several methods. All Macrhybopsis species consumed mostly midge pupae, but M. meeki had the most general diet (Levins’ index, B=0⋅22) compared with M. hyostoma (B=0⋅02) and M. gelida (B=0⋅09). Morisita’s diet overlap index among species pairs ranged from 0⋅62 to 0⋅97 and was highest between M. hyostoma and M. gelida. Daily ages estimated from lapilli otoliths for each species ranged from 15 to 43 days for M. gelida, 19 to 44 for …

Noninvasive Measurement Of Electrical Events Associated With A Single Chlorovirus Infection Of A Microalgal Cell, Seung-Woo Lee, Eun-Hee Lee, Gerhard Thiel, James L. Van Etten, Ravi Saraf

Department of Chemical and Biomolecular Engineering: Faculty Publications

Chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) contains a viral-encoded K⁺ channel imbedded in its internal membrane, which triggers host plasma membrane depolarization during virus infection. This early stage of infection was monitored at high resolution by recording the cell membrane depolarization of a single Chlorella cell during infection by a single PBCV-1 particle. The measurement was achieved by depositing the cells onto a network of one-dimensional necklaces of Au nanoparticles, which spanned two electrodes 70 μm apart. The nanoparticle necklace array has been shown to behave as a single-electron device at room temperature. The resulting electrochemical field-effect transistor …

Temporal Dynamics Of Maize Plant Growth, Water Use, And Leaf Water Content Using Automated High Throughput Rgb And Hyperspectral Imaging, Yufeng Ge, Geng Bai, Vincent Stoerger, James C. Schnable

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Automated collection of large scale plant phenotype datasets using high throughput imaging systems has the potential to alleviate current bottlenecks in data-driven plant breeding and crop improvement. In this study, we demonstrate the characterization of temporal dynamics of plant growth and water use, and leaf water content of two maize genotypes under two different water treatments. RGB (Red Green Blue) images are processed to estimate projected plant area, which are correlated with destructively measured plant shoot fresh weight (FW), dry weight (DW) and leaf area. Estimated plant FW and DW, along with pot weights, are used to derive daily plant …

Recalibration Methodology To Compensate For Changing Fluid Properties In An Individual Nozzle Direct Injection Systems, Joe D. Luck, S. A. Shearer, B. D. Luck, M. P. Sama

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Limited advancement of direct injection pesticide application systems has been made in recent years, which has hindered further commercialization of this technology. One approach to solving the lag and mixing issues typically associated with injection-based systems is high-pressure individual nozzle injection. However, accurate monitoring of the chemical concentrate flow rate can pose a challenge due to the high pressure, low flow, and changing viscosities of the fluid. A methodology was developed for recalibrating high-pressure chemical concentrate injectors to compensate for fluid property variations and evaluate the performance of this technique for operating injectors in an open-loop configuration. Specific objectives were …

Pch1 Integrates Circadian And Light- Signaling Pathways To Control Photoperiod-Responsive Growth In Arabidopsis, He Huang, Chan Yul Yoo, Rebecca Bindbeutel, Jessica Goldsworthy, Allison Tielking, Sophie Alvarez, Michael J. Naldrett, Bradley S. Evans, Meng Chen, Dmitri A. Nusinow

Nebraska Center for Biotechnology: Faculty and Staff Publications

Plants react to seasonal change in day length through altering physiology and development. Factors that function to harmonize growth with photoperiod are poorly understood. Here we characterize a new protein that associates with both circadian clock and photoreceptor components, named PHOTOPERIODIC CONTROL OF HYPOCOTYL1 (PCH1). pch1 seedlings have overly elongated hypocotyls specifically under short days while constitutive expression of PCH1 shortens hypocotyls independent of day length. PCH1 peaks at dusk, binds phytochrome B (phyB) in a red light-dependent manner, and co-localizes with phyB into photobodies. PCH1 is necessary and sufficient to promote the biogenesis of large photobodies to maintain an …

Biolabeling Through The Use Of Water-Soluble Colloidal Quantum Dots, Cody Stombaugh

Honors Projects

Nanomaterials continues to be a growing field of study due to their wide range of potential applications. Quantum dots are artificially synthesized crystalline clusters of atoms able to confine electron motion as a result of their incredibly small size. Recently, medical applications of nanomaterials have expanded greatly. Quantum dots are ideal for biolabeling due to their rather narrow photoluminescence emission peaks. By synthesizing quantum dots of a specific diameter, it is possible to predetermine the peak photoluminescence wavelength of a sample. Through ligand exchange and immunoconjugation of the quantum dots with proteins, it is possible to use the quantum dots …

Triclocarban Influences Antibiotic Resistance And Alters Anaerobic Digester Microbial Community Structure, Daniel Elliott Carey, Daniel Zitomer, Krassimira R. Hristova, Anthony D. Kappell, Patrick J. Mcnamara

Civil and Environmental Engineering Faculty Research and Publications

Triclocarban (TCC) is one of the most abundant organic micropollutants detected in biosolids. Lab-scale anaerobic digesters were amended with TCC at concentrations ranging from the background concentration of seed biosolids (30 mg/kg) to toxic concentrations of 850 mg/kg to determine the effect on methane production, relative abundance of antibiotic resistance genes, and microbial community structure. Additionally, the TCC addition rate was varied to determine the impacts of acclimation time. At environmentally relevant TCC concentrations (max detect = 440 mg/kg), digesters maintained function. Digesters receiving 450 mg/kg of TCC maintained function under gradual TCC addition, but volatile fatty acid concentrations increased, …

Density Functional Theory Calculations On The Doping Sequence Of Mnxfen(1-X)Ge, Jordan C. Ball

Honors Capstones

No abstract provided.

Aconitic Acid, Glycerol And Cinnamic Acid Polyester Characterization For Bone Tissue Engineering, Katie Joanna Hogan

Honors Capstones

No abstract provided.

An Analysis Of Plasticity In The Rat Respiratory System Following Cervical Spinal Cord Injury And The Application Of Nanotechnology To Induce Or Enhance Recovery Of Diaphragm Function, Janelle Lorien Walker

Wayne State University Dissertations

Second cervical segment spinal cord hemisection (C2Hx) results in ipsilateral hemidiaphragm paralysis. However, the intact latent crossed phrenic pathway can restore function spontaneously over time or immediately following drug administration.

WGA bound fluorochromes were administered to identify nuclei associated with diaphragm function in both the acute and chronic C2Hx models. WGA is unique in that it undergoes receptor mediated endocytosis and is transsynaptically transported across select physiologically active synapses. Comparison of labeling in the acutely injured to the chronically injured rat provided an anatomical map of spinal and supraspinal injury induced synaptic plasticity. The plasticity occurs over time in the …

Development Of An Elderly Female Torso Finite Element Model For Restraint System Research And Development Applications, Anil Kalra Kalra

Wayne State University Dissertations

Elderly females are found to be one of the most vulnerable segments of population during motor vehicle crashes and their population is increasing in the coming decades. Current design of restraint systems as well as other passive safety measures are designed based on anthropomorphic details of younger population. Developing another dummy representing elderly female population is a costly effort, while a finite element (FE) model of elderly female with similar anthropomorphic details and age- and gender-specific material properties can be a better alternative solution. The current research focuses on the development of a FE model of an elderly female torso, …

Nanosecond Pulsed Platelet-Rich Plasma (Nsprp) Improves Mechanical And Electrial Cardiac Function Following Myocardial Reperfusion Injury, Barbara Y. Hargrave, Frency Varghese, Nektarios Barabutis, John Catravas, Christian Zemlin

School of Medical Diagnostics & Translational Sciences Publications

Ischemia and reperfusion (I/R) of the heart is associated with biochemical and ionic changes that result in cardiac contractile and electrical dysfunction. In rabbits, platelet-rich plasma activated using nanosecond pulsed electric fields (nsPRP) has been shown to improve left ventricular pumping. Here, we demonstrate that nsPRP causes a similar improvement in mouse left ventricular function. We also show that nsPRP injection recovers electrical activity even before reperfusion begins. To uncover the mechanism of nsPRP action, we studied whether the enhanced left ventricular function in nsPRP rabbit and mouse hearts was associated with increased expression of heat-shock proteins and altered mitochondrial …

Accessible Bioprinting: Adaptation Of A Low-Cost 3d-Printer For Precise Cell Placement And Stem Cell Differentiation, John A. Reid, Peter A. Mollica, Garett D. Johnson, Roy C. Ogle, Robert D. Bruno, Patrick C. Sachs

School of Medical Diagnostics & Translational Sciences Publications

The precision and repeatability offered by computer-aided design and computer-numerically controlled techniques in biofabrication processes is quickly becoming an industry standard. However, many hurdles still exist before these techniques can be used in research laboratories for cellular and molecular biology applications. Extrusion-based bioprinting systems have been characterized by high development costs, injector clogging, difficulty achieving small cell number deposits, decreased cell viability, and altered cell function post-printing. To circumvent the high-price barrier to entry of conventional bioprinters, we designed and 3D printed components for the adaptation of an inexpensive 'off-the-shelf' commercially available 3D printer. We also demonstrate via goal based …

Subject-Specific Biomechanics Of Trunk: Musculoskeletal Scaling, Internal Loads And Intradiscal Pressure Estimation, F. Ghezelbash, A. Shirazi-Adl, N. Arjmand, Z. El-Ouaaid, A. Plamondon

Études primaires

Development of a subject-specific computational musculoskeletal trunk model (accounting for age, sex, body weight and body height), estimation of muscle forces and internal loads as well as subsequent validation by comparison with measured intradiscal pressure in various lifting tasks are novel, important and challenging. The objective of the present study is twofold. First, it aims to update and personalize the passive and active structures in an existing musculoskeletal kinematics-driven finite element model. The scaling scheme used an existing imaging database and biomechanical principles to adjust muscle geometries/cross-sectional-areas and passive joint geometry/properties in accordance with subjects’ sex, age, body weight and …

Bioactivity/Cytotoxicity Of Micro-/Nano-Materials And Novel Development Of Fiber-Optic Probes For Single Cell Monitoring, Qingbo Yang

Doctoral Dissertations

"Manufactured nano-/micro-materials (MNMs) have been widely used and their interactions with niche biological environment are highly concerned for both of their biohazardous and bioactive effects, whereas no available comprehensive evaluations or regulations have been provided yet. This dissertation thus focuses on three major aspects: 1) fundamental toxicity understandings of a typical MNMs (zinc oxide nanoparticles), 2) bioactivity evaluations of representative bioactive MNMs, and 3) development of novel micro-probes for high spatial resolution monitoring. Firstly, the NP's concentration, irradiation, hydrodynamic size, and the localized pH, ionic strength, NP zeta-potential as well as dissolved oxygen levels were found correlated with the production …

Fluorescent Nanocomposite Of Embedded Ceria Nanoparticles In Crosslinked Pva Electrospun Nanofibers, Nader Shehata, Soha Gaballah, Effat Samir, Aya Hamed, Marwa Saad

Biology Faculty Publications

This paper introduces a new fluorescent nanocomposite of electrospun biodegradable nanofibers embedded with optical nanoparticles. In detail, this work introduces the fluorescence properties of PVA nanofibers generated by the electrospinning technique with embedded cerium oxide (ceria) nanoparticles. Under near-ultra violet excitation, the synthesized nanocomposite generates a visible fluorescent emission at 520 nm, varying its intensity peak according to the concentration of in situ embedded ceria nanoparticles. This is due to the fact that the embedded ceria nanoparticles have optical tri-valiant cerium ions, associated with formed oxygen vacancies, with a direct allowed bandgap around 3.5 eV. In addition, the impact of …

Flexc: Protein Flexibility Prediction Using Context-Based Statistics, Predicted Structural Features, And Sequence Information, Ashraf Yaseen, Mais Nijim, Brandon Williams, Lei Qian, Min Li, Jianxin Wang, Yaohang Li

Computer Science Faculty Publications

The fluctuation of atoms around their average positions in protein structures provides important information regarding protein dynamics. This flexibility of protein structures is associated with various biological processes. Predicting flexibility of residues from protein sequences is significant for analyzing the dynamic properties of proteins which will be helpful in predicting their functions.