Engineering Commons^™

04. Botany, Northeastern State University

Oklahoma Research Day Abstracts

No abstract provided.

Jainism And Nonviolence: From Mahavira To Modern Times, Lana E. Sims

The Downtown Review: An Interdisciplinary Journal Written and Peer-Reviewed by Mandel Honors College Students at Cleveland State University

The research contained in this paper focuses on the religion of Jainism, its main principle of ahimsa, or nonviolence, and how that philosophy has evolved over the years, eventually inspiring today’s nonviolent movements. First, a look will be taken at the origin of Jainism, with a brief explanation of its most important principles and beliefs. Then, ahimsa is explained as it has been traditionally perceived by Jains. The recent evolution of the meaning of ahimsa and its impact on modern nonviolence movements is discussed, with a focus on the influence of second-generation Jains in the United States on the …

03. Biology, Northeastern State University

Oklahoma Research Day Abstracts

No abstract provided.

Stem Gateway Course Redesign Teaching Professional Development: Resources For Teaching And Learning, Gary Smith, Audriana Stark

STEM Gateway

The learning object is a collection of teaching professional development presentations and workbooks for guiding faculty in the re-design of lower-division college science and mathematics courses. The materials were designed and implemented during the University of New Mexico STEM Gateway Project, funded by the U.S. Department of Education Title V program during 2012-2016. The teaching professional development curriculum consisted of a 2.5-day course redesign institute followed by roughly monthly sessions on topics that include teaching diverse students; building learning strategies for students; obtaining student buy-in for active learning; evaluating alignment of learning objectives, activities, and assessment; peer observation of teaching, …

H.F. Hauff Pruner Housing & Ergonomics, Erich E. Heilman

All Undergraduate Projects

The technology of electric tree pruners in the current agricultural market has many engineering problems, including; a deficit in power output, excessive overheating, and ergonomics. The 3-man pruner team has coordinated with H.F. Hauff Co. Inc., an agricultural equipment company owned by Neil Hauff, to produce an electric pruner prototype which addresses these problems. The methods used to design the optimal pruner prototype includes traditional engineering stress analysis, force flow analysis, finite element software, and Solidworks modeling software. The construction of the pruner involved manual machining methods and C.N.C. (Computerized Numerical Control) mill technology. During the design process, the team …

02. Animal Science, Northeastern State University

Oklahoma Research Day Abstracts

No abstract provided.

Cold Atmospheric Plasma Inhibits Hiv-1 Replication In Macrophages By Targeting Both The Virus And The Cells., Olga Volotskova, Larisa Dubrovsky, Michael Keidar, Michael Bukrinsky

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Cold atmospheric plasma (CAP) is a specific type of partially ionized gas that is less than 104°F at the point of application. It was recently shown that CAP can be used for decontamination and sterilization, as well as anti-cancer treatment. Here, we investigated the effects of CAP on HIV-1 replication in monocyte-derived macrophages (MDM). We demonstrate that pre-treatment of MDM with CAP reduced levels of CD4 and CCR5, inhibiting virus-cell fusion, viral reverse transcription and integration. In addition, CAP pre-treatment affected cellular factors required for post-entry events, as replication of VSV-G-pseudotyped HIV-1, which by-passes HIV receptor-mediated fusion at the plasma …

Exploring Agricultural Production Systems And Their Fundamental Components With System Dynamics Modelling, Jeffrey P. Walters, David W. Archer, Gretchen F. Sassenrath, John R. Hendrickson, Jon D. Hanson, John M. Halloran, Peter Vadas, Vladimir J. Alarcon

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

Agricultural production in the United States is undergoing marked changes due to rapid shifts in consumer demands, input costs, and concerns for food safety and environmental impact. Agricultural production systems are comprised of multidimensional components and drivers that interact in complex ways to influence production sustainability. In a mixed-methods approach, we combine qualitative and quantitative data to develop and simulate a system dynamics model that explores the systemic interaction of these drivers on the economic, environmental and social sustainability of agricultural production. We then use this model to evaluate the role of each driver in determining the differences in sustainability …

Ex-Vivo Slaughterhouse Porcine Crystalloid-Perfused Beating Heart Via Langendorff Method, Rahiemin Talukder

Electronic Theses and Dissertations

The objective of this study was to resuscitate isolated beating porcine hearts obtained from slaughterhouse swine for a minimum of 1 hour with a crystalloid buffer reperfusion instead of blood via an extracorporeal Langendorff apparatus. The isolated beating heart functions outside of the body under simulated physiologic conditions. Live functional anatomy was controlled under benchtop experimental settings. Porcine hearts (26 total) were topically cooled with saline. 300mL of 25°C cardioplegia with 20KU Streptokinase was preflushed via aortic root. WIT was less than 5 minutes. 1L 4°C cardioplegia was flushed antegrade with aortic cannula (Group 1) or coronary catheterization (Group 2). …

Journal Of The Arkansas Academy Of Science - Volume 70 2016, Academy Editors

Journal of the Arkansas Academy of Science

No abstract provided.

Light Scattering Characterization Of Elastin-Like Polypeptide Trimer Micelles, Ilona Tsuper, Daniel Terrano, Adam Maraschky

Undergraduate Research Posters 2016

Elastin-Like Polypeptides (ELP) can be used to form thermo-reversible vehicles for drug delivery systems. The ELP nanoparticles are composed of three-armed star polypeptides. Each of the three arms extending from the negatively charged foldon domain includes 20 repeats of the (GVGVP) amino acid sequence. The ELP polymer chains are soluble at room temperature and become insoluble at the transition temperature (close to 50 oC), forming micelles. The size and shape of the micelle is dependent on the temperature and the pH of solution, along with the concentration of the Phosphate Buffered Saline (PBS) solvent. The technique of Depolarized Dynamic Light …

Design And Fabrication Of A Microfluidic Device Using A 3d Printer, Aaron Smith

Undergraduate Research Posters 2016

The goal of this work was to design and implement a microfluidic mixer-based chemical reactor using a 3-D printer. Microfluidic devices are very useful for processes that use expensive reactants or require a high level of control, and we hypothesize that the ability to develop a prototype using a 3-D printer would lower their costs and help overcome some of the limitations of soft-lithography based devices. One-level and two-level designs were made using SolidWorks® software, and various stages of the model were printed on Objet® and FDM® printers. The Objet printer offers the advantage of printing a prototype using a …

Light Scattering Study Of Mixed Micelles Made From Elastin-Like Polypeptide Linear Chains And Trimers, Ilona Tsuper, Daniel Terrano, Adam Maraschky

Undergraduate Research Posters 2016

Temperature sensitive nanoparticles (E20F) were generated from a construct of three chains of Elastin- Like Polypeptides (ELP) linked to a negatively charged foldon domain. This ELP system was mixed at different ratios with a single linear chain of ELP (H40L) which was deprived of the foldon domain. The mixed system is soluble at room temperature and at a transition temperature will form swollen micelles with the hydrophobic linear chains hidden inside. This system was studied using Depolarized Dynamic Light Scattering (DDLS) and Static Light Scattering (SLS) to model the size, shape, and internal structure of the mixed micelles. The mixed …

Life Behind Glass: Bioreactor Studies On The Salt-Water Adaptation Of Scenedesmus Dimorphus, Joshua Hartranft, Alex Fedai, Sahar Ataya

Undergraduate Research Posters 2016

Algae has considerable promise as source for liquid biofuel because of high productivity and because algae farms do not compete with food crops for arable land. Freshwater algae is preferred over saltwater algae because of the higher lipid content in the former. Unfortunately, freshwater algae “farms” would require a vast amount of fresh water, and given the scarcity of fresh water, this is not a viable long-term solution. Therefore, the adaption of a fresh-water species of algae to a salt-water environment is a potential solution. In this study, it was shown that the growth rate of the freshwater algae S. …

Scalable Assembly Of Nanoparticles Onto Templated Substrates, John Juchnowski

Undergraduate Research Posters 2016

Anisotropic nanoparticles, such as carbon nanotubes and noble metal nanorods, have excellent electrical, mechanical, and thermal properties. This work examined techniques for the nanopatterning of silicon substrates for the subsequent deposition of anisotropic nanoparticles in order to exploit their properties in macroscopic applications. Argon-ion-sputter induced rippling of Si (100) as well as a microparticle mask were examined as methods for templating a silicon substrate. For the ion-sputter

Identifying Patterns Of Stress Through Biological Markers, Kristyn Oravec

Undergraduate Research Posters 2016

technique, the angle of incidence of the ion beam was 67° from the surface normal,

Novel Approaches For Assessment Of Copper Toxicity: Fast Scan Cyclic Voltammetry And Optical Bioassays, Annette R. Tremonti

Wayne State University Dissertations

Anthropogenic activities negatively impact fresh water ecosystems through toxic contaminants that are released into the environment. Copper (Cu) is a water contaminant that is fundamentally persistent once introduced into the environment that has the potential for bioaccumulation. Although Cu toxicity has been studied for decades, there is still a continuing problem with new sources and pathways. New approaches are needed to understand distribution and transport of Cu and its potential for complex biological impacts beyond the simple assessment of lethality. Several novel approaches were used in this research project to advance our understanding of Cu toxicity, including fast scan cyclic …

Glycerolipid Analysis Of Adaptation To Saline Changes In The Culture Conditions Of Algae, Scenedesmus Dimorphus, By Gc-Ms, Tyler Fitzgerald, Satya Girish Chandra Avula, Chandana Mannem

Undergraduate Research Posters 2016

Since fossil fuels are decreasing over time an alternative energy source will be soon required. The algae, Scenedesmus dimorphus, grows in freshwater and is known for its fast growth of glycerolipid content which is used for biodiesel production. After the algae is grown in optimal conditions, the released fatty acids and glycerolipids are transformed into fatty acid methyl esters (FAMEs) which are used as biodiesel. The FAMEs were quantitatively determined by gas chromatography mass spectrometry (GC-MS) to determine the total glycerolipid content in the different algae samples. The samples that were analyzed include freshwater controls and saline adapted samples. Analysis …

Broadband And Mobile Internet Services In Nonmetropolitan Nebraska, Rebecca Vogt, Cheryl Burkhart-Kriesel, Randolph Cantrell, Bradley Lubben, L. J. Mcelravy

Rural Futures Institute: Publications

A recent Pew Research Center survey revealed that home broadband adoption has plateaued in the United States. In addition, there has been an increase in adults who use a smartphone to access the Internet but do not have traditional broadband service in their home. Given these national trends, how do rural Nebraskans access the Internet? Are they accessing the Internet from their cell phones? Are they subscribing to high-speed Internet services at home? How satisfied are they with their Internet services? How important do they feel Internet access is for various items? This paper provides a detailed analysis of these …

Thermodynamics And Kinetics Of The Three-Way Junction Of Phi29 Motor Prna And Its Assembly Into Nanoparticles For Therapeutic Delivery To Prostate Cancer, Daniel W. Binzel

Theses and Dissertations--Pharmacy

The emerging field of RNA nanotechnology necessitates creation of functional RNA nanoparticles, but has been limited by particle instability. Previously, it was found the three-way junction (3WJ) of the Phi29 DNA packaging motor pRNA was found to be ultra-stable and assemble in solution without the presence of metal ions. The three-way junction is composed of three short oligo RNA strands and proven to be thermodynamically stable. Here the assembly mechanism, thermodynamic and enzymatic stabilities, and kinetics are examined in order to understand the stability behind this unique motif. Thermodynamic and kinetics studies found that the pRNA 3WJ formed out of …

A Study Of Alterations In Dna Epigenetic Modifications (5mc And 5hmc) And Gene Expression Influenced By Simulated Microgravity In Human Lymphoblastoid Cells, Basudev Chowdhury, Arun Seetharam, Zhiping Wang, Yunlong Liu, Amy C. Lossie, Jyothi Thimmapuram

Cyber Center Publications

Cells alter their gene expression in response to exposure to various environmental changes. Epigenetic mechanisms such as DNA methylation are believed to regulate the alterations in gene expression patterns. In vitro and in vivo studies have documented changes in cellular proliferation, cytoskeletal remodeling, signal transduction, bone mineralization and immune deficiency under the influence of microgravity conditions experienced in space. However microgravity induced changes in the epigenome have not been well characterized. In this study we have used Next-generation Sequencing (NGS) to profile ground-based “simulated” microgravity induced changes on DNA methylation (5-methylcytosine or 5mC), hydroxymethylation (5-hydroxymethylcytosine or 5hmC), and simultaneous gene …

Underwater Explosion Energy Dissipation Near Waterborne Infrastructure, Paul R. Smith

Theses and Dissertations--Civil Engineering

Underwater explosions pose a significant threat to waterborne infrastructure though destructive pressure waves that can travel significant distances through the water. However, the use of bubble screens can attenuate the peak pressure and energy flux created by explosions to safe levels. This study investigates the prediction of pressure wave characteristics based on accumulated data, the damage potential of underwater explosions based on applied loads and effective material strength, and the bubble screen parameters required to prevent damage. The results were compiled to form a procedure for the design and implementation of a bubble screen the protection of waterborne infrastructure.

Lifetime And Effectiveness Evaluation Of Ceramic Pot Filters, Carlo Salvinelli

Doctoral Dissertations

"Poor water quality is a major contributing factor to disease in developing countries. Ceramic pot filters (CPFs) represent an effective and sustainable technology for poor communities, but the characterization of CPF lifetimes is on-going, and the water production seems to be the limiting factor. This dissertation describes laboratory and field investigations conducted to characterize the parameters that impact CPF effectiveness and lifetime in terms of water production and treatment efficacy, both under controlled and real use conditions.

CPF initial flow rate is the most common quality control parameter, but it may not be representative of the long-term effectiveness of the …

Modeling The Compressibility Behavior Of Hard Red Wheat Varieties, Aaron P. Turner, Michael D. Montross, Samuel G. Mcneill, Michael P. Sama, Mark E. Casada, Josephine M. Boac, Rumela Bhadra, Ronaldo G. Maghirang, Sidney A. Thompson

Biosystems and Agricultural Engineering Faculty Publications

The bulk density of grain in a storage structure varies vertically and horizontally due to the overburden pressure created by the cumulative weight of the overlying material. As the overburden pressure increases, the stored material compacts. This compaction is believed to be caused by rearrangement of kernels along with higher intergranular stress between particles, leading to kernel deformation. This compaction is of primary concern when estimating the amount of grain in a storage structure. In this comprehensive study, confined uniaxial compression tests were conducted on 27 different samples of hard red winter wheat, at three moisture levels, over the range …

Error Analysis Of Stored Grain Inventory Determination, Aaron P. Turner, Michael D. Montross, Joshua J. Jackson, Samuel G. Mcneill, Mark E. Casada, Josephine M. Boac, Rumela Bhadra, Ronaldo G. Maghirang, Sidney A. Thompson

Biosystems and Agricultural Engineering Faculty Publications

Estimation of the quantity of stored grain is important for crop insurance, financial statements, and inventory control. Traditionally, the height of grain has been measured using weighted tape measures, and the volume is subsequently computed using standard geometric shapes (cylinders and cones) along with visual correction of the grain surface. Field measurements by four trained USDA Farm Service Agency and crop insurance agents on older farm-sized bins (8.2 to 11.0 m, or 27 to 36 ft, in diameter) resulted in standard deviations between 0.02 and 0.30 m for the equivalent height when the grain surface was not level. The largest …

Correlating Bulk Density (With Dockage) And Test Weight (Without Dockage) For Wheat Samples, Rumela Bhadra, Mark E. Casada, Josephine M. Boac, Aaron P. Turner, Sidney A. Thompson, Michael D. Montross, Ronaldo G. Maghirang, Samuel G. Mcneill

Biosystems and Agricultural Engineering Faculty Publications

In grain bins, the compaction of stored grain is caused by the overbearing pressure of the bulk material in the bin. To predict the amount of grain in the bin, compaction values must be determined based on the average bulk density (BD) of the stored material. However, BD is determined following the Federal Grain Inspection Service (FGIS) guidelines for measuring test weight (TW), which require that dockage be removed prior to measuring wheat TW. Thus, this creates a problem for predicting grain compaction and conducting inventory studies, because the average BD of the grain in a bin for these calculations …

As-Applied Estimation Of Volumetric Flow Rate From A Single Sprayer Nozzle Series Using Water-Sensitive Spray Cards, Michael P. Sama, John T. Evans, Aaron P. Turner, Surya Saket Dasika

Biosystems and Agricultural Engineering Faculty Publications

The objective of this study was to test the feasibility of using coverage measurements from water-sensitive spray cards to estimate the volumetric flow rate at an individual sprayer nozzle. TeeJet VisiFlow Even Flat Spray Tips were selected due to their uniform distribution of coverage. Spray distribution for each nozzle was validated using a spray patternator table with 2.5 cm sampling widths. A rotary test fixture translated water-sensitive spray cards through the spray dispersion (water at ambient conditions) at a constant angular velocity and a radius of 1.2 m. The test fixture measured volumetric flow and pressure at the nozzle and …

A Review Of Non-Destructive Methods For Detection Of Insect Infestation In Fruits And Vegetables, Nader Ekramirad, Akinbode A. Adedeji, Reza Alimardani

Biosystems and Agricultural Engineering Faculty Publications

Insect damage in fruits and vegetables cause major production and economic losses in the agriculture and food industry worldwide. Monitoring of internal quality and detection of insect infestation in fruits and vegetables is critical for sustainable agriculture. Early detection of an infestation in fruits can facilitate the control of insects and the quarantine operations through proper post-harvest management strategies and can improve productivity. The present review recognizes the need for developing a rapid, cost-effective, and reliable insect infestation monitoring system that would lead to advancements in agriculture and food industry. In this paper, an overview of non-destructive detection insect damages …

Nozzle Sensor For In-System Chemical Concentration Monitoring, Joseph S. Dvorak, Timothy S. Stombaugh, Yongbo Wan

Biosystems and Agricultural Engineering Faculty Publications

Chemical concentration is a vital parameter for determining appropriate chemical application. This study describes the design and testing of a sensor that attempted to monitor concentration of chemicals upstream from each nozzle body. The sensor is based on an LED and photodiode pair. Its ability to detect chemical concentration within the main carrier was tested with a 2,4-D formulation, a glyphosate formulation, and a powdered Acid Blue 9 dye. The liquid herbicide formulations of glyphosate and 2,4-D were tested across common application concentrations of 0% to 12.5% by volume. The powdered dye produced a much stronger effect on the sensor …

Size Specific Transfection To Mammalian Cells By Micropillar Array Electroporation, Yingbo Zu

Doctoral Dissertations

Electroporation serves as a promising non-viral gene delivery approach, while its current configurations carry drawbacks associated with high-voltage electrical pulses and heterogeneous treatment on individual cells. Here, we developed a new micropillar array electroporation (MAE) platform to advance the delivery of plasmid DNA and RNA to mammalian cells. By introducing well-patterned micropillar array on the electrode surface, the number of pillars each cell faces varies with its cell membrane surface area, despite their large population and random locations. In this way, cell size specific electroporation is conveniently done and contributed to a 2.5~3 fold increase on plasmid DNA transfection and …