Engineering Commons^™

Measuring Volatile Emissions From Moss Gametophytes: A Review Of Methodologies And New Applications, Danlyn L. Brennan, Leslie M. Kollar, Scott Kiel, Timea Deakova, Aurélie Laguerre, Stuart F. Mcdaniel, Sarah Eppley, Elliott T. Gall, Todd Rosenstiel

Center for Life in Extreme Environments Publications

Mosses inhabit nearly all terrestrial ecosystems and engage in important interactions with nitrogen-fixing microbes, sperm-dispersing arthropods, and other plants. It is hypothesized that these interactions could be mediated by biogenic volatile organic compounds (BVOCs). Moss BVOCs may play fundamental roles in influencing local ecologies, such as biosphere–atmosphere–hydrosphere communications, physiological and evolutionary dynamics, plant–microbe interactions, and gametophyte stress physiology. Further progress in quantifying the composition, magnitude, and variability of moss BVOC emissions, and their response to environmental drivers and metabolic requirements, is limited by methodological and analytical challenges. We review several sampling techniques with various analytical approaches and describe best practices …

Genes Involved In Cell Division In Acinetobacter Baumannii Are Coregulated By Umudab And Ddrr, Belinda Candra, Xiaohong Li, Eric Rouchka, Deborah Cook, Janelle Hare

2022 Celebration of Student Scholarship - Poster Presentations

In the presence of DNA damage, the multi-drug resistant bacteria Acinetobacter baumannii employs the proteins UmuDAb and DdrR to repress the expression of error-prone polymerases.

Bistability And Switching Behavior In Moving Animal Groups, Daniel Strömbom, Stephanie Nickerson, Catherine Futterman, Alyssa Difazio, Cameron Costello, Kolbjørn Tunstrøm

Northeast Journal of Complex Systems (NEJCS)

Moving animal groups such as schools of fish and flocks of birds frequently switch between different group structures. Standard models of collective motion have been used successfully to explain how stable groups form via local interactions between individuals, but they are typically unable to produce groups that exhibit spontaneous switching. We are only aware of one model, constructed for barred flagtail fish that are known to rely on alignment and attraction to organize their collective motion, that has been shown to generate this type of behavior in 2D (or 3D). Interestingly, another species of fish, golden shiners, do exhibit switching …

Synthesis Of Thiol-Acrylate Hydrogels For 3d Cell Culture And Microfluidic Applications, Anowar Hossain Khan

LSU Doctoral Dissertations

Globally cell culture is an $18.98 billion industry as of 2020, with an 11.6 percent annual growth rate. Drug discovery has an estimated worth of $69.8 billion in 2020 and is predicted to grow to $110.4 billion by 2025. Three-dimensional (3D) cell culture of cancer cells is one of the rapidly growing felids since it better recapitulates in vivo conditions compared to two-dimensional (2D) models. However, it is challenging to grow 3D tumor spheroids outside the body, and some of the existing technology can generate these spheroids outside the human body but poorly mimic in vivo tumor models. Therefore, there …

Development Of Enhanced Algae Strains And Sustainable Cultivation Processes For Production Of Algal Lipids For Biofuels And Bioproducts, Enlin Lo

USF Tampa Graduate Theses and Dissertations

The establishment of an algae industry is crucial for addressing global biofuel and bioproduct demand that meets the Sustainable Development Goals (SDG) worldwide. Photosynthetic microalgae are excellent sources for food, fiber, fuel, feed, and fertilizer, which are the 5Fs of the SDG. However, at present, algae-based materials are not cost-competitive or sufficiently sustainable. Improved productivity, lower cultivation cost, and reduced use of resources are required to transition from lab to industry, necessitating the development of superior strains that have high productivity and can tolerate environmental stress, such as temperature and salinity, so they can be cultivated at large scale outdoors …

Seabem: An Artificial Intelligence Powered Web Application To Predict Cover Crop Biomass, Aime Christian Tuyishime, Andrea Basche

Honors Program: Senior Projects (Public)

SEABEM, the Stacked Ensemble Algorithms Biomass Estimator Model, is a web application with a stacked ensemble of Machine Learning (ML) algorithms running on the backend to predict cover crop biomass for locations in Sub-Saharan. The SEABEM model was developed using a previously developed database of crop growth and yield that included site characteristics such as latitude, longitude, soil texture (sand, silt, and clay percentages), temperature, and precipitation. The goal of SEABEM is to provide global farmers, mainly small-scale African farmers, the knowledge they need before practicing and benefiting from cover crops while avoiding the expensive and time-consuming operations that come …

A Non-Iteration Solution For Solving The Backward-In-Time Two-Dimensional Burgers’ Equation With A Large Reynolds Number, Yen-Shen Chang, Yung-Wei Chen, Chein-Shan Liu, Jiang-Ren Chang

Journal of Marine Science and Technology–Taiwan

This article proposes a noniteration solution based on the Lie-group shooting method (LGSM) to solve the backward-in-time two-dimensional Burgers’ equation with a large Reynolds number. The backward problem is famous for seriously ill-posed cases because the solution is generally unstable and highly dependent on the input data. Small perturbations in the input data, such as random errors inherent to the measurements in the analysis, can cause large oscillations in the solution. To handle a large Reynolds number under long time spans, it is very difficult to integrate towards the time direction. To avoid time integration and numerical iteration, the noniteration …

Polyketides And Terpenes In Marine Microalgae: Ecological Roles And Mechanisms Of Biosynthesis, Yunxuan Xie, Mohammad Elsheikh

Journal of Marine Science and Technology–Taiwan

Marine microalgae comprise a large number of polyketides and terpenes identified to date with highly diverse molecular scaffolds and the biological activities (Kalaitzis et al., 2010). These compounds play significant roles in marine food web and nutraceutical supplementation in marine animals. Compounds of this class are often synthesized by gene clusters with dedicated domains responsible for carbon chain extension and modification. The unique molecular structure of these compounds encourages people to investigate their biosynthetic mechanisms in-depth. In this review, we summarize relevant articles to unravel the shared mechanisms of these compounds to further understand the variety of domain organizations and …

Unbaited Light-Emitting Diode Traps Performance For Catching Orange Mud Crabs, Muhamad Naimullah, Kuo-Wei Lan, Mhd Ikhwanuddin, Adnan Amin-Safwan, Wei-Yu Lee

Journal of Marine Science and Technology–Taiwan

Mud crabs (Scylla spp.) are a vital fishery resource and targeted for a valuable source of income for coastal communities throughout the Indo-Pacific region. Baited traps are a considerable expense in crustacean fishing. Hence, the present study was performed to investigate the catchability of Scylla olivacea in response to light-emitting diodes (LEDs) in captivity and field conditions. We used a new experimental setup in the captivity condition that revealed most S. olivacea were attracted to green, blue, and white LED lights and no attractive effect by red LED lights similar to the controls. Field studies have shown that the catch …

Catch And Effort Standardization For Taiwanese Swordtip Squid Uroteuthis Edulis Fisheries In The Southern East China Sea, Ke-Yang Chang, Yu-Jie Hsu, Tzu-Yun Ching, Cheng-Hsin Liao, Chih-Shin Chen

Journal of Marine Science and Technology–Taiwan

The swordtip squid Uroteuthis edulis is a neritic squid species that is widely distributed within the coastal waters of subtropical and tropical Indo-western Pacific regions. This squid is a commercially important species throughout its distribution range and plays a critical role in marine ecosystems. Stock assessments of the squid in the East China Sea (ECS) are scarce, although it is crucial information for the sustainability of the squid and relevant fisheries. The squid was harvested by various fisheries in the southern ECS, which could restrict methods for catch-effort standardization. In this study, catch-effort data of Taiwanese swordtip squid fisheries in …

Uniform Torsion Analysis Of Composite Shafts Using Point Collocation Method Based On Pascal Polynomials, Chih-Wen Chang, Jyh-Hong Wu, Ying-Ru Lin, Yu-Feng Lin, Nan-Nong Huang

Journal of Marine Science and Technology–Taiwan

Torsion bars are important elements in the marine structure applications. The usage of the composite torsion bars has increased recently because it takes advantage of the different material property of each constituent. The current research focuses mainly on the uniform warping analysis of composite torsion shafts by using the point collocation method (PCM). The PCM is based on Pascal's triangle polynomial expansion and coupled with subdomain technique that is indispensable for the analysis of composite bars. In addition to satisfying the field equation (Laplace equation for warping function) and traction-free boundary condition for each component, the continuity conditions in both …

Local Reinforcement Design And Safety Factor Evaluation Of Elliptical Hatch Of Pressure Hull, Guang-Min Luo, Jun-Wei Chen

Journal of Marine Science and Technology–Taiwan

A pressure hull is the primary pressure-resistant structure of a submarine. To safeguard the compressive strength of such a hull, local structural reinforcement is required for various hatches on the hull. Hatches are commonly reinforced through increasing the thickness of the hull regions around them; alternatively, hatch nozzle walls are connected with stiffened frames to maintain the continuity of reinforced structures. This study focused on a pressure hull with an elliptical hatch. A PD-5500 cylindrical pressure hull was used to design a cylindrical pressure hull structure, and a model of hatch nozzle reinforcement was designed using the Boiler and Pressure …

Effects Of Dietary Lipid, L-Carnitine And L-Lysine Supplementation On The Growth Performance And Body Composition Of Epinephelus Lanceolatus Larvae, Jen-Hong Chu, Yu-Cong Hong, Angela Chien, Shyn-Shin Sheen

Journal of Marine Science and Technology–Taiwan

The effects of different combinations of dietary lipid, L-carnitine and L-lysine on the growth performance and fatty acid profiles of larval grouper Epinephalus lanceolatus were evaluated. Eight treatment diets were formulated based on a 2x2x2 factorial design, namely 5 and 14% dietary oil mixture (3:1 fish oil/soybean oil), 0 and 0.5% L-carnitine and 0 and 2.83% L-lysine. Each diet was randomly assigned to triplicate groups of ten 0.08g E. lanceolatus larvae for 42 days. The grouper larvae fed with diets containing 14% lipid had significantly higher weight gain percentages than those fed with diets containing 5% lipid. The weight gain …

Antimicrobial Activity Of Zinc Oxide Nanoparticles Compared To Silver Nanoparticles Administered Towards Methicillin-Resistant Staphylococcus Aureus Biofilms, Kevin Núño

USF Tampa Graduate Theses and Dissertations

Zinc oxide nanoparticles (ZnO NPs), silver nanoparticles (AgNPs), and chitosan nanoparticles have strong antibacterial activity as a broad-spectrum antibiotic against multi-drug resistant (MDR) bacteria. MDR bacteria have a mechanism that increases antibiotic resistance through biofilm formations. MDR methicillin-resistant Staphylococcus aureus BAA-1720 was chosen for an in vitro assessment to calculate the biofilm reduction rate and the median effective dose (ED50) by performing biofilm quantification. Dynamic light scattering was chosen to determine the physiochemical characteristics of ZnO NPs, silver nanoparticles (AgNPs), and chitosan NPs. Transmission electron microscopy was conducted to verify each treatment’s physiochemical characteristics. Antimicrobial susceptibility testing of broad-spectrum and …

The Optimal Relationship Between Actuator Stiffness And Actuation Timing For A Passive Ankle Exoskeleton: An Opensim Simulation, Cody Anderson, Hafizur Rahman, Sara Myers

UNO Student Research and Creative Activity Fair

Introduction

The passive ankle exoskeleton developed by Collins et al. (2015) reduced the metabolic cost of walking with an actuation-timing of ~16% of stance [1]; however, other actuation timings have not been extensively investigated. Therefore, the purpose of this study was to determine the optimal relationship between actuator-stiffness and actuation-timing for a passive ankle exoskeleton by using musculoskeletal modeling.

Methods

Kinematics and ground reaction forces were recorded while a healthy-young male walked on overground force-plates, and these data were exported to a musculoskeletal modeling software (OpenSim) for simulation. A passive ankle exoskeleton model was designed and integrated with a default …

Design And Development Of Software With A Graphical User Interface To Display And Convert Multiple Microscopic Histology Images, Sayed Ahmadreza Razian, Majid Jadidi, Alexey Kamenskiy

UNO Student Research and Creative Activity Fair

Histological images are widely used to assess the microscopic anatomy of biological tissues. Recent advancements in image analysis allow the identification of structural features on histological sections that can help advance medical device development, brain and cancer research, drug discovery, vascular mechanobiology, and many other fields. Histological slide scanners create images in SVS and TIFF formats that were designed to archive image blocks and high-resolution textual information. Because these formats were primarily intended for storage, they are often not compatible with conventional image analysis software and require conversion before they can be used in research. We have developed a user-friendly …

Breaks In Longitudinal Elastic Fibers Of Human Femoropopliteal Arteries, Elham Zamani

UNO Student Research and Creative Activity Fair

Breaks in Longitudinal Elastic Fibers of Human Femoropopliteal Arteries

Elham Zamani1, Majid Jadidi1

1 Department of Biomechanics, University of Nebraska Omaha, Omaha, NE

Introduction: Elastin is a major protein in the body with half-life >50 years. It is thought that elastic fibers are formed before the postnatal period. In the femoropopliteal artery (FPA), the main artery in the leg, longitudinal elastic fibers are present in External Elastic Lamina (EEL). Our team has studied more than 1000 cadaveric human FPA and has noticed that there are big breaks in their longitudinal elastic fibers in some subjects. Our goal in this work …

Low-Cost Prosthetic Feet For Underserved Populations: A Comparison Of Gait Analysis And Mechanical Stiffness, Kaleigh Renninger, Brevin Banks, Dustin A. Bruening

Library/Life Sciences Undergraduate Poster Competition 2022

• Lower limb loss is an ongoing cause of disability throughout the world.
• Despite advancements in prosthetic technologies, there are numerous underserved populations in need of effective low-cost prosthetic foot options.

Hsp90 Inhibitors Modulate Sars-Cov-2 Spike Protein Subunit 1-Induced Human Pulmonary Microvascular Endothelial Activation And Barrier Dysfunction, Ruben Manuel Luciano Colunga Biancatelli, Pavel Solopov, Betsy W. Gregory, Yara Khodour, John D. Catravas

Bioelectrics Publications

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused more than 5 million deaths worldwide. Multiple reports indicate that the endothelium is involved during SARS-Cov-2-related disease (COVID-19). Indeed, COVID-19 patients display increased thrombophilia with arterial and venous embolism and lung microcapillary thrombotic disease as major determinants of deaths. The pathophysiology of endothelial dysfunction in COVID-19 is not completely understood. We have investigated the role of subunit 1 of the SARS-CoV-2 spike protein (S1SP) in eliciting endothelial barrier dysfunction, characterized dose and time relationships, and tested the hypothesis that heat shock protein 90 (HSP90) inhibitors would prevent and repair such injury. S1SP …

Impacts Of Temperature And Carbon Dioxide On Boro Rice Production, Sabiha Ferdous

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

There are different anticipated impacts of climate change (CC) on agriculture all over the world. Bangladesh as a developing country has potential vulnerability and negative impacts on CC. This change in meteorological parameters creates negative impacts on agricultural production which is a threat to food security and economic development. The country has recently achieved food security in rice which is mainly due to increased yields and area coverage of irrigated dry season (Boro) rice over the last decades. It is necessary to predict the future Boro rice production in accordance with CC as well as take supplementary actions to ensure …

Mytrials: Clinical Trial Application Concept, Katie Hollister

Graphic Communication

A clinical trial’s success is dependent upon whether a patient attends their highly-structured clinical trial visit schedule, which may include both in-person clinic visits and virtual check-in visits. MyTrials is a clinical trial journey app prototype that enables patients to schedule, reschedule, and attend join their in-person or virtual clinic visits. This product seeks to reduce the burden that scheduling places on the patient, and provides trial-provided resources and a daily and weekly timeline to help patients navigate their clinical trial journey.

Spatial And Temporal Variation Of Selected Water Quality Parameters In The Tanzanian Side Of Lake Victoria, Richard Joseph Kimwaga

Tanzania Journal of Engineering and Technology (TJET)

Lake Victoria’s water quality is increasingly becoming under heavy pressure mainly due to land based activities and aerial inputs which are taking place within the basin. This study was carried out to assess the spatial, including vertical and temporal, variation of the water quality of Lake Victoria on the Tanzanian side. Historical water quality data for the period from 2000 to 2016 was collected. Temperature, pH, Dissolved Oxygen (DO) and Turbidity were selected for study since they are the mostly measured and monitored water quality parameters. Land use-land cover changes were analysed using ArcGIS. ILWIS 3.7 software was used to …

The Low Abundance Of Cpg In The Sars-Cov-2 Genome Is Not An Evolutionarily Signature Of Zap, Ali Afrasiabi, Hamid Alinejad-Rokny, Azad Khosh, Mostafa Rahnama, Nigel Lovell, Zhenming Xu, Diako Ebrahimi

Plant Pathology Faculty Publications

The zinc finger antiviral protein (ZAP) is known to restrict viral replication by binding to the CpG rich regions of viral RNA, and subsequently inducing viral RNA degradation. This enzyme has recently been shown to be capable of restricting SARS-CoV-2. These data have led to the hypothesis that the low abundance of CpG in the SARS-CoV-2 genome is due to an evolutionary pressure exerted by the host ZAP. To investigate this hypothesis, we performed a detailed analysis of many coronavirus sequences and ZAP RNA binding preference data. Our analyses showed neither evidence for an evolutionary pressure acting specifically on CpG …

Prostacyclin Promotes Degenerative Pathology In A Model Of Alzheimer’S Disease, Tasha R. Womack, Craig T. Vollert, Odochi Ohia-Nwoko, Monika Schmitt, Saghi Montazari, Tina L. Beckett, David Mayerich, M. Paul Murphy, Jason L. Eriksen

Molecular and Cellular Biochemistry Faculty Publications

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that is the most common form of dementia in aged populations. A substantial amount of data demonstrates that chronic neuroinflammation can accelerate neurodegenerative pathologies. In AD, chronic neuroinflammation results in the upregulation of cyclooxygenase and increased production of prostaglandin H2, a precursor for many vasoactive prostanoids. While it is well-established that many prostaglandins can modulate the progression of neurodegenerative disorders, the role of prostacyclin (PGI2) in the brain is poorly understood. We have conducted studies to assess the effect of elevated prostacyclin biosynthesis in a mouse model of AD. Upregulated prostacyclin expression …

Therapeutic Treatment With The Anti-Inflammatory Drug Candidate Mw151 May Partially Reduce Memory Impairment And Normalizes Hippocampal Metabolic Markers In A Mouse Model Of Comorbid Amyloid And Vascular Pathology, David J. Braun, David K. Powell, Christopher J. Mclouth, Saktimayee M. Roy, D. Martin Watterson, Linda J. Van Eldik

Neuroscience Faculty Publications

Alzheimer's disease (AD) is the leading cause of dementia in the elderly, but therapeutic options are lacking. Despite long being able to effectively treat the ill-effects of pathology present in various rodent models of AD, translation of these strategies to the clinic has so far been disappointing. One potential contributor to this situation is the fact that the vast majority of AD patients have other dementia-contributing comorbid pathologies, the most common of which are vascular in nature. This situation is modeled relatively infrequently in basic AD research, and almost never in preclinical studies. As part of our efforts to develop …

Behavioral Responses Of Blue-Winged Teal And Northern Shoveler To Unmanned Aerial Vehicle Surveys, Mason D. Ryckman, Kaylan Kemink, Christopher J. Felege, Brian J. Darby, Gregory S. Vandeberg, Susan N. Ellis-Felege

Biology Faculty Publications

Unmanned aerial vehicles (UAVs) have become a popular wildlife survey tool. Most research has focused on detecting wildlife using UAVs with less known about behavioral responses. We compared the behavioral responses of breeding blue-winged teal (Spatula discors) (n = 151) and northern shovelers (Spatula clypeata) (n = 46) on wetlands flown over with a rotary DJI Matrice 200 quadcopter and control wetlands without flights. Using a GoPro camera affixed to a spotting scope, we conducted focal individual surveys and recorded duck behaviors for 30 minutes before, during, and 30 minutes after UAV flights to determine if …

Transcranial Direct Current Stimulation On Parkinson’S Disease: Systematic Review And Meta-Analysis, Paloma Cristina Alves De Oliveira, Thiago Anderson Brito De Araújo, Daniel Gomes Da Silva Machado, Abner Cardoso Rodrigues, Marom Bikson, Suellen Marinho Andrade, Alexandre Hideki Okano, Hougelle Simplicio, Rodrigo Pegado, Edgard Morya

Publications and Research

Background: Clinical impact of transcranial direct current stimulation (tDCS) alone for Parkinson’s disease (PD) is still a challenge. Thus, there is a need to synthesize available results, analyze methodologically and statistically, and provide evidence to guide tDCS in PD.

Objective: Investigate isolated tDCS effect in different brain areas and number of stimulated targets on PD motor symptoms.

Methods: A systematic review was carried out up to February 2021, in databases: Cochrane Library, EMBASE, PubMed/MEDLINE, Scopus, and Web of science. Full text articles evaluating effect of active tDCS (anodic or cathodic) vs. sham or control on motor symptoms of PD were …

Rapid Response: Cat-Scan Machine And 3d Printers Vital In Covid-19, Laura Meader

Colby Magazine

Colby’s Assistant Professor of Biology Josh Martin is using his expertise and sophisticated scientific equipment to create face shields, respirator masks, and air filter cartridges for local first responders and hospitals in need of equipment that is crucial to treating patients with COVID-19.

Glycocalyx Mechanotransduction Mechanisms Are Involved In Renal Cancer Metastasis, Heriberto Moran, Limary M. Cancel, Peigen Huang, Sylvie Roberge, Tuoye Xu, John M. Tarbell, Lance L. Munn

Publications and Research

Mammalian cells, including cancer cells, are covered by a surface layer containing cell bound proteoglycans, glycoproteins, associated glycosaminoglycans and bound proteins that is commonly referred to as the glycocalyx. Solid tumors also have a dynamic fluid microenvironment with elevated interstitial flow. In the present work we further investigate the hypothesis that interstitial flow is sensed by the tumor glycocalyx leading to activation of cell motility and metastasis. Using a highly metastatic renal carcinoma cell line (SN12L1) and its low metastatic counterpart (SN12C) we demonstrate in vitro that the small molecule Suberoylanilide Hydroxamic Acid (SAHA) inhibits the heparan sulfate synthesis enzyme …

Detecting The Feeding Habitat Zone Of Albacore Tuna (Thunnus Alalunga) In The Southern Indian Ocean Using Multisatellite Remote Sensing Data, Sandipan Mondal, Yang-Chi Lan, Ming-An Lee, Yi-Chen Wang, Bambang Semedi, Wan-Ya Su

Journal of Marine Science and Technology–Taiwan

Remote sensing is an effective method for identifying potential feeding habitat zones. Spatial and temporal distribution patterns of albacore (ALB) tuna were studied using Taiwan longline fisheries data from 2009 to 2014. A generalized additive model (GAM) was used to compile a fishery database and statistically explore the relationship between distribution and environmental factors. Sea surface temperature (SST), sea surface chlorophyll-a concentration (SSC), sea surface salinity (SSS), sea surface height (SSH), mixed layer depth (MLD), and eddy kinetic energy (EKE) were examined. The results indicated SST as the largest contributor on ALB distribution, followed by SSS and SSC. Catches per …