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Full-Text Articles in Engineering

Case Study: Modeling A Grain Bin For Safe Entry Retrofit, Michael Dyer, Serap Gorucu, Randall Bock, Roderick Thomas, Jude Liu, Linda Fetzer Apr 2023

Case Study: Modeling A Grain Bin For Safe Entry Retrofit, Michael Dyer, Serap Gorucu, Randall Bock, Roderick Thomas, Jude Liu, Linda Fetzer

Office of Research Faculty and Staff Publications

All new grain bins produced after 2018 are recommended to have anchor points capable of handling a 2000 lb loading for attachment of bin entry lifeline systems. This study aims to assess the feasibility of a safe entry anchor point retrofit by using finite element analysis (FEA). We used a grain bin owned by Penn State for 3D FEA modeling in SolidWorks. To validate the model results from the FEA model, first strain and then deflection measurements were conducted on the grain. Strain gauges were applied to the grain bin in five locations and strain values were obtained after applying …


Mechanical Properties And Morphological Alterations In Fiber-Based Scaffolds Affecting Tissue Engineering Outcomes, James Dolgin, Samerender Nagam Hanumantharao, Stephen Farias, Carl G. Simon, Smitha Rao Apr 2023

Mechanical Properties And Morphological Alterations In Fiber-Based Scaffolds Affecting Tissue Engineering Outcomes, James Dolgin, Samerender Nagam Hanumantharao, Stephen Farias, Carl G. Simon, Smitha Rao

Michigan Tech Publications

Electrospinning is a versatile tool used to produce highly customizable nonwoven nanofiber mats of various fiber diameters, pore sizes, and alignment. It is possible to create electrospun mats from synthetic polymers, biobased polymers, and combinations thereof. The post-processing of the end products can occur in many ways, such as cross-linking, enzyme linking, and thermal curing, to achieve enhanced chemical and physical properties. Such multi-factor tunability is very promising in applications such as tissue engineering, 3D organs/organoids, and cell differentiation. While the established methods involve the use of soluble small molecules, growth factors, stereolithography, and micro-patterning, electrospinning involves an inexpensive, labor …


Deficit Irrigation Management For Irrigated Corn In Nebraska: Economically Viable?, Lia Nogueira, Cory Walters, Emily O'Donnell, Wesley Peterson, Suat Irmak Apr 2023

Deficit Irrigation Management For Irrigated Corn In Nebraska: Economically Viable?, Lia Nogueira, Cory Walters, Emily O'Donnell, Wesley Peterson, Suat Irmak

Cornhusker Economics

In this study we determine the economic value of deficit irrigation management using both technological and methodological advancements. The use of soil moisture probes represents the technological improvement. We provide improvements in the methodology as follows. Regarding data, we employ a field-size study, instead of plots, where the irrigation decision is determined by the moisture level in the soil measured through a soil moisture probe. Regarding the understanding of the yield response to water, although we examine the commonly used quadratic function, we improve upon this specification by also examining an alternative response function, the linear response stochastic plateau. Our …


Human Microbiome Transfer In The Built Environment Differs Based On Occupants, Objects, And Buildings, Andrew J. Hoisington, Christopher E. Stamper, Katherine L. Bates, Maggie A. Stanislawski, Michael C. Flux, Teodor T. Postolache, Christopher A. Lowry, Lisa A. Brenner Apr 2023

Human Microbiome Transfer In The Built Environment Differs Based On Occupants, Objects, And Buildings, Andrew J. Hoisington, Christopher E. Stamper, Katherine L. Bates, Maggie A. Stanislawski, Michael C. Flux, Teodor T. Postolache, Christopher A. Lowry, Lisa A. Brenner

Faculty Publications

Compared to microbiomes on other skin sites, the bacterial microbiome of the human hand has been found to have greater variability across time. To increase understanding regarding the longitudinal transfer of the hand microbiome to objects in the built environment, and vice versa, 22 participants provided skin microbiome samples from their dominant hands, as well as from frequently and infrequently touched objects in their office environments. Additional longitudinal samples from home environments were obtained from a subset of 11 participants. We observed stability of the microbiomes of both the hand and built environments within the office and home settings; however, …


Characterization Of Physical And Biochemical Traits In Wheat And Corn Plants Using High Throughput Image Analysis, Kantilata Thapa Apr 2023

Characterization Of Physical And Biochemical Traits In Wheat And Corn Plants Using High Throughput Image Analysis, Kantilata Thapa

Department of Biological Systems Engineering: Dissertations and Theses

Plant phenotyping has been recognized as a rapidly growing field of research due to the labor-intensive, destructive, and time-consuming nature of traditional phenotyping methods. These phenotyping bottlenecks can be addressed by advancements in image-based phenotyping like RGB and hyperspectral imaging for the assessment of plant traits important for breeding purposes. This study aims (1) to characterize the physical and biochemical traits of wheat and corn plants using RGB and hyperspectral imaging in the greenhouse, and (2) to estimate leaf nitrogen (N), phosphorus (P), and potassium (K) content using hyperspectral imaging and an analytical spectral device (ASD spectrometer) and compare the …


Flexible Materials And Applications For Wearable Sensors, Brock Ferrari Apr 2023

Flexible Materials And Applications For Wearable Sensors, Brock Ferrari

Senior Honors Theses

This literature review aimed to address the limitations of rigid wearable sensors in the medical community by investigating the development of flexible materials for remote health monitoring. A keyword search was conducted on Google Scholar, PubMed, and the Jerry Falwell Library, which yielded 9,102 articles. After applying filtering techniques, the results were narrowed down to 21 articles, which were categorized into "Present Market Conditions," "Flexible Materials for Medical Use," "Applications for Wearable Sensors," and "Potential Use Cases." Discussions were held on prominent materials such as substrate, nanocomposite, and liquid metal materials, exploring their potential applications for chemical and physical sensing, …


Establishment Of Wildflower Islands To Enhance Roadside Health, Ecological Value, And Aesthetics Phase Ii, Jackson Ebbers, Heidi L. Hillhouse, John A. Guretsky, Thomas J. Weissling Mar 2023

Establishment Of Wildflower Islands To Enhance Roadside Health, Ecological Value, And Aesthetics Phase Ii, Jackson Ebbers, Heidi L. Hillhouse, John A. Guretsky, Thomas J. Weissling

Nebraska Department of Transportation: Research Reports

No abstract provided.


Enhanced Internal Coating Structure And Light Reflectance Of Coated Papers: A Sludge Valorization Process, Bilge N. Altay, Anamika Huq, Burak Aksoy, Richard Hailstone, Muslum Demir, Cem Aydemir, Arif Karademir, Scott Williams Mar 2023

Enhanced Internal Coating Structure And Light Reflectance Of Coated Papers: A Sludge Valorization Process, Bilge N. Altay, Anamika Huq, Burak Aksoy, Richard Hailstone, Muslum Demir, Cem Aydemir, Arif Karademir, Scott Williams

Articles

Paper mill sludge (PMS) is a high-volume waste of the pulppapermaking industry, containing cellulose fibers. This paper introduces cellulose nanofibers extracted from PMS (CNFsPMS) and their application in paper coatings using a wire-wound coating method to enhance internal and optical properties of paper. Three factors were investigated: (i) CNFsPMS amount, (ii) number of layers, and (iii) surface treatment via a 4 × 2 × 2 factorial design. The experimental responses were tracked by fundamentally important internal properties including porosity, air permeability, thickness, roughness, and optical properties of brightness, gloss, and CIE L*a*b* color coordination. The results showed that CNFPMS enhances …


Split And Join: An Efficient Approach For Simulating Stapled Intestinal Anastomosis In Virtual Reality, Di Qi, Suvranu De Feb 2023

Split And Join: An Efficient Approach For Simulating Stapled Intestinal Anastomosis In Virtual Reality, Di Qi, Suvranu De

Engineering Faculty Articles and Research

Colorectal cancer is a life-threatening disease. It is the second leading cause of cancer-related deaths in the United States. Stapled anastomosis is a rapid treatment for colorectal cancer and other intestinal diseases and has become an integral part of routine surgical practice. However, to the best of our knowledge, there is no existing work simulating intestinal anastomosis that often involves sophisticated soft tissue manipulations such as cutting and stitching. In this paper, for the first time, we propose a novel split and join approach to simulate a side-to-side stapled intestinal anastomosis in virtual reality. We mimic the intestine model using …


Editorial: Special Issue “Protein Modeling And Simulation: Selected Articles From The Computational Structural Bioinformatics Workshop 2021”, Negin Forouzesh, Kamal Ai Nasr Feb 2023

Editorial: Special Issue “Protein Modeling And Simulation: Selected Articles From The Computational Structural Bioinformatics Workshop 2021”, Negin Forouzesh, Kamal Ai Nasr

Computer Science Faculty Research

No abstract provided.


Nanoclays‑Containing Bio‑Based Packaging Materials: Properties, Applications, Safety, And Regulatory Issues, Kalpani Y. Perera, Maille Hopkins, Amit K. Jaiswal Dr, Swarna Jaiswal Feb 2023

Nanoclays‑Containing Bio‑Based Packaging Materials: Properties, Applications, Safety, And Regulatory Issues, Kalpani Y. Perera, Maille Hopkins, Amit K. Jaiswal Dr, Swarna Jaiswal

Articles

Food packaging is an important concept for consumer satisfaction and the increased shelf life of food products. The introduction of novel food packaging materials has become an emerging trend in recent years, which could be mainly due to environmental pollution caused by plastic packaging and to reduce food waste. Recently, numerous studies have been carried out on nanoclays or nanolayered silicate to be used in packaging material development as reinforcing filler composites. Different types of nanoclays have been used as food packaging materials, while montmorillonite (MMT), halloysite, bentonite (BT), Cloisite, and organically modified nanoclays have become of great interest. The …


A Review Of 3d Polymeric Scaffolds For Bone Tissue Engineering: Principles, Fabrication Techniques, Immunomodulatory Roles, And Challenges, Hassan Nageh, Ahmed G. Abdelaziz, Sara M. Abdo, Mohga S. Abdalla, Asmaa A. Amer, Abdalla Abdal-Hay, Ahmed Barhoum Feb 2023

A Review Of 3d Polymeric Scaffolds For Bone Tissue Engineering: Principles, Fabrication Techniques, Immunomodulatory Roles, And Challenges, Hassan Nageh, Ahmed G. Abdelaziz, Sara M. Abdo, Mohga S. Abdalla, Asmaa A. Amer, Abdalla Abdal-Hay, Ahmed Barhoum

Nanotechnology Research Centre

Over the last few years, biopolymers have attracted great interest in tissue engineering and regenerative medicine due to the great diversity of their chemical, mechanical, and physical properties for the fabrication of 3D scaffolds. This review is devoted to recent advances in synthetic and natural polymeric 3D scaffolds for bone tissue engineering (BTE) and regenerative therapies. The review comprehensively discusses the implications of biological macromolecules, structure, and composition of polymeric scaffolds used in BTE. Various approaches to fabricating 3D BTE scaffolds are discussed, including solvent casting and particle leaching, freeze-drying, thermally induced phase separation, gas foaming, electrospinning, and sol–gel techniques. …


Development Of A Porous Layer-By-Layer Microsphere With Branched Aliphatic Hydrocarbon Porogens, Farah Shahjin, Milankumar Patel, Mahmudul Hasan, Jacob D. Cohen, Farhana Islam, Md Ashaduzzaman, Mohammad Ullah Nayan, Mahadevan Subramaniam, You Zhou, Irene Andreu, Howard E. Gendelman, Bhavesh D. Kevadiya Feb 2023

Development Of A Porous Layer-By-Layer Microsphere With Branched Aliphatic Hydrocarbon Porogens, Farah Shahjin, Milankumar Patel, Mahmudul Hasan, Jacob D. Cohen, Farhana Islam, Md Ashaduzzaman, Mohammad Ullah Nayan, Mahadevan Subramaniam, You Zhou, Irene Andreu, Howard E. Gendelman, Bhavesh D. Kevadiya

Nebraska Center for Biotechnology: Faculty and Staff Publications

Porous polymer microspheres are employed in biotherapeutics, tissue engineering, and regenerative medicine. Porosity dictates cargo carriage and release that are aligned with the polymer physicochemical properties. These include material tuning, biodegradation, and cargo encapsulation. How uniformity of pore size affects therapeutic delivery remains an area of active investigation. Herein, we characterize six branched aliphatic hydrocarbon-based porogen(s) produced to create pores in single and multilayered microspheres. The porogens are composed of biocompatible polycaprolactone, poly(lactic-co-glycolic acid), and polylactic acid polymers within porous multilayered microspheres. These serve as controlled effective drug and vaccine delivery platforms.


The Impact Of Organized Activism On Technology Sharing, Ahmed Chennak, Amalia Yiannaka Jan 2023

The Impact Of Organized Activism On Technology Sharing, Ahmed Chennak, Amalia Yiannaka

Cornhusker Economics

What factors influence a firm's decision to share its technology with competitors? While patent licensing has received considerable attention in the literature, studies have typically focused on the optimal licensing contract for product and process innovations in various market settings. A key finding of this literature is that the optimal licensing strategy depends on the type of the patentee; that is, whether the patentee is a producer in the market (insider patentee) or not (outsider patentee). Little attention has been given, however, to the role the general public can play on firms' incentives to share their innovations through licensing contracts …


Role Of Social Network On Technology Adoption: Application To Nebraska Producers In The Face Of Undesirable Vegetation Transitions, Sabrina Gulab, Holly K. Nesbitt, Simanti Banerjee, Theresa Floyd Jan 2023

Role Of Social Network On Technology Adoption: Application To Nebraska Producers In The Face Of Undesirable Vegetation Transitions, Sabrina Gulab, Holly K. Nesbitt, Simanti Banerjee, Theresa Floyd

Cornhusker Economics

Conclusion

Producers need to have access to information regarding new conservation practices and technologies to ensure land management in the face of ecological threats in general and vegetation transitions (VTs) in the context of our study. This study investigates the role of an individual producer's social network on the willingness to seek information about technologies and management practices and the likelihood of new technology adoption with special attention to risk attitudes and producer spillover effects. Our results provide evidence that network composition and information obtained through a producer's social network don't influence an individual's willingness to seek information about new …


Clutch Analysis And Design For The Wildcat Pulling Team Quarter Scale Tractor Drivetrain, Lauren Doyle Jan 2023

Clutch Analysis And Design For The Wildcat Pulling Team Quarter Scale Tractor Drivetrain, Lauren Doyle

Lewis Honors College Thesis Collection

The University of Kentucky (UK) Wildcat Pulling Team competes in the annual ASABE International Quarter Scale Tractor Student Design Competition. In 2022 the team placed 3rd overall in the competition, missing 1st place by 23 points. The loss in points was due to the clutch subassembly having issues related to maneuverability, manufacturability, and serviceability. The goal of this project was to redesign the 2022 tractor’s drivetrain for the new 2023 competition tractor. Background research was conducted on three types of clutches: manual, electric, and centrifugal. The electric clutch and centrifugal clutch with manual override were tested to determine their viability …


Economic And Input-Use Efficiency Of Nitrogen Management Techniques In Non-Irrigated Maize Production, Katie J. Bathke Jan 2023

Economic And Input-Use Efficiency Of Nitrogen Management Techniques In Non-Irrigated Maize Production, Katie J. Bathke

Honors Theses

The efficiency of nitrogen (N) management has become a main concern in agricultural cropping systems for understanding the optimal N rate to help producers improve economically and reduce the exhaustion of natural resources environmentally. Nitrogen rates vary both temporally and spatially by the interactions of the soil environment and rainfall through a growing season. Thus, a site-specific approach can further optimize this variability with the understanding of the role soil variability plays in the N rate applied to a non-irrigated field. The objective of this study was to evaluate the various nitrogen treatments for their nutrient use efficiency and economic …


Marineepi: A Gui-Based Matlab Toolbox To Simulate Marine Pathogen Transmission, Gorka Bidegain, Tal Ben-Horin, Eric N. Powell, John M. Klinck, Eileen E. Hofmann Jan 2023

Marineepi: A Gui-Based Matlab Toolbox To Simulate Marine Pathogen Transmission, Gorka Bidegain, Tal Ben-Horin, Eric N. Powell, John M. Klinck, Eileen E. Hofmann

CCPO Publications

The Graphical User Interface (GUI) MarineEpi is presented as a Matlab toolbox for easily (i) constructing disease transmission models for different marine host-pathogen systems, (ii) running simulations by specifying initial conditions and model parameters, and (iii) interpreting the resulting time series of the host and pathogen population dynamics. MarineEpi users can generate models for systems in which pathogen transmission occurs through contact with infected individuals (SI), contact with dead infected individuals (SID), contact with environmental pathogens released by infected individuals (SIP), and contact with environmental pathogens released by dead infected individuals (SIPD). MarineEpi is a freely available GUI that provides …


Swosu Research And Scholarly Activity Fair 2023, Swosu Office Of Sponsored Programs Jan 2023

Swosu Research And Scholarly Activity Fair 2023, Swosu Office Of Sponsored Programs

SWOSU Research and Scholarly Activity Fair Programs

On behalf of the members of the University Research and Scholarly Activity Committee (USRAC) and the Office of Sponsored Programs (OSP) at Southwestern Oklahoma State University (SWOSU) - Welcome to the Thirtieth SWOSU Research and Scholarly Activity Fair! There are 77poster presentations and 2 oral presentations involving over 130 student and faculty researchers, writers, presenters, artists, collaborators, and faculty sponsors encompassing activities from the SWOSU College of Pharmacy; SWOSU School of Nursing & Allied Health Sciences; and SWOSU Departments of Allied Health Sciences; Art, Communication & Theatre; Biological Sciences; Business; Computer Science; Chemistry & Physics; Engineering Technology; Language & Literature; …


Conductive 3d Nano-Biohybrid Systems Based On Densified Carbon Nanotube Forests And Living Cells, Roya Bagheri, Alicia K. Ball, Masoud Kasraie, Aparna Chandra, Xinqian Chen, Ibrahim Miskioglu, Zhiying Shan, Parisa Pour Shahid Saeed Abadi Jan 2023

Conductive 3d Nano-Biohybrid Systems Based On Densified Carbon Nanotube Forests And Living Cells, Roya Bagheri, Alicia K. Ball, Masoud Kasraie, Aparna Chandra, Xinqian Chen, Ibrahim Miskioglu, Zhiying Shan, Parisa Pour Shahid Saeed Abadi

Michigan Tech Publications, Part 2

Conductive biohybrid cell-material systems have applications in bioelectronics and biorobotics. To date, conductive scaffolds are limited to those with low electrical conductivity or 2D sheets. Here, 3D biohybrid conductive systems are developed using fibroblasts or cardiomyocytes integrated with carbon nanotube (CNT) forests that are densified due to interactions with a gelatin coating. CNT forest scaffolds with a height range of 120–240 µm and an average electrical conductivity of 0.6 S/cm are developed and shown to be cytocompatible as evidenced from greater than 89% viability measured by live-dead assay on both cells on day 1. The cells spread on top and …


Impact Of Atmospheric Correction On Classification And Quantification Of Seagrass Density From Worldview-2 Imagery, Victoria J. Hill, Richard C. Zimmerman, Paul Bissett, David Kohler, Blake Schaeffer, Megan Coffer, Jiang Li, Kazi Aminul Islam Jan 2023

Impact Of Atmospheric Correction On Classification And Quantification Of Seagrass Density From Worldview-2 Imagery, Victoria J. Hill, Richard C. Zimmerman, Paul Bissett, David Kohler, Blake Schaeffer, Megan Coffer, Jiang Li, Kazi Aminul Islam

OES Faculty Publications

Mapping the seagrass distribution and density in the underwater landscape can improve global Blue Carbon estimates. However, atmospheric absorption and scattering introduce errors in space-based sensors’ retrieval of sea surface reflectance, affecting seagrass presence, density, and above-ground carbon (AGCseagrass) estimates. This study assessed atmospheric correction’s impact on mapping seagrass using WorldView-2 satellite imagery from Saint Joseph Bay, Saint George Sound, and Keaton Beach in Florida, USA. Coincident in situ measurements of water-leaving radiance (Lw), optical properties, and seagrass leaf area index (LAI) were collected. Seagrass classification and the retrieval of LAI were compared after empirical line …


Study Of Legionella Pneumophila Treatment With Copper In Drinking Water By Single Cell-Icp-Ms, Lei Xu, Austin Sigler, Anna Chernatynskaya, Lindsey Rasmussen, Jingrang Lu, Endalkachew Sahle-Demessie, David J. Westenberg, Hu Yang, Honglan Shi Jan 2023

Study Of Legionella Pneumophila Treatment With Copper In Drinking Water By Single Cell-Icp-Ms, Lei Xu, Austin Sigler, Anna Chernatynskaya, Lindsey Rasmussen, Jingrang Lu, Endalkachew Sahle-Demessie, David J. Westenberg, Hu Yang, Honglan Shi

Biological Sciences Faculty Research & Creative Works

Legionella pneumophila is a persistent opportunistic pathogen that poses a significant threat to domestic water systems. Previous studies suggest that copper (Cu) is an effective antimicrobial in water systems. A rapid and sensitive quantification method is desired to optimize the conditions of L. pneumophila treatment by Cu and to better understand the interaction mechanisms between Cu and cells. In this study, we developed a highly sensitive single cell (SC)-ICP-MS method to monitor L. pneumophila cell concentration and track their uptake of Cu. The SC-ICP-MS method showed excellent sensitivity (with a cell concentration detection limit of 1000 cells/mL), accuracy (good agreement …


Thermotrophy Exploratory Study, James Weifu Lee Jan 2023

Thermotrophy Exploratory Study, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

The question of whether environmental heat energy could be utilized as a source of energy for biological metabolism is the center of this exploratory research. In 1979, this author postulated a hypothesis for the existence of thermotrophs that could isothermally utilize environmental heat energy as a source of their energy on Earth. According to this hypothesis, the thermotrophs could be the first primitive forms of life in the early Earth environment. The chemotrophs and phototrophs that we currently are all well familiar with might have been evolved somehow from the primitive thermotrophs. Furthermore, all the organisms currently regarded as the …


Neutrosophic Superhyperalgebra And New Types Of Topologies, Florentin Smarandache, Memet Sahin, Derya Bakbak, Vakkas Uluçay, Abdullah Kargı Jan 2023

Neutrosophic Superhyperalgebra And New Types Of Topologies, Florentin Smarandache, Memet Sahin, Derya Bakbak, Vakkas Uluçay, Abdullah Kargı

Branch Mathematics and Statistics Faculty and Staff Publications

In general, a system S (that may be a company, association, institution, society, country, etc.) is formed by sub-systems Si { or P(S), the powerset of S }, and each sub-system Si is formed by sub-sub-systems Sij { or P(P(S)) = P^2(S) } and so on.

That’s why the n-th PowerSet of a Set S { defined recursively and denoted by P^n(S) = P(P^(n-1)(S) } was introduced, to better describes the organization of people, beings, objects etc. in our real world.

The n-th PowerSet was used in defining the SuperHyperOperation, SuperHyperAxiom, and their corresponding Neutrosophic SuperHyperOperation, Neutrosophic SuperHyperAxiom in order …


A Single-Cell Atlas Of Bovine Skeletal Muscle Reveals Mechanisms Regulating Intramuscular Adipogenesis And Fibrogenesis, Leshan Wang, Peidong Gao, Chaoyang Li, Qianglin Liu, Zeyang Yao, Yuxia Li, Xujia Zhang, Jiangwen Sun, Constantine Simintiras, Matthew Welborn, Kenneth Mcmillin, Stephanie Oprescu, Shihuan Kuang, Xing Fu Jan 2023

A Single-Cell Atlas Of Bovine Skeletal Muscle Reveals Mechanisms Regulating Intramuscular Adipogenesis And Fibrogenesis, Leshan Wang, Peidong Gao, Chaoyang Li, Qianglin Liu, Zeyang Yao, Yuxia Li, Xujia Zhang, Jiangwen Sun, Constantine Simintiras, Matthew Welborn, Kenneth Mcmillin, Stephanie Oprescu, Shihuan Kuang, Xing Fu

Computer Science Faculty Publications

Background

Intramuscular fat (IMF) and intramuscular connective tissue (IMC) are often seen in human myopathies and are central to beef quality. The mechanisms regulating their accumulation remain poorly understood. Here, we explored the possibility of using beef cattle as a novel model for mechanistic studies of intramuscular adipogenesis and fibrogenesis.

Methods

Skeletal muscle single-cell RNAseq was performed on three cattle breeds, including Wagyu (high IMF), Brahman (abundant IMC but scarce IMF), and Wagyu/Brahman cross. Sophisticated bioinformatics analyses, including clustering analysis, gene set enrichment analyses, gene regulatory network construction, RNA velocity, pseudotime analysis, and cell-cell communication analysis, were performed to elucidate …


Dfhic: A Dilated Full Convolution Model To Enhance The Resolution Of Hi-C Data, Bin Wang, Kun Liu, Yaohang Li, Jianxin Wang Jan 2023

Dfhic: A Dilated Full Convolution Model To Enhance The Resolution Of Hi-C Data, Bin Wang, Kun Liu, Yaohang Li, Jianxin Wang

Computer Science Faculty Publications

Motivation: Hi-C technology has been the most widely used chromosome conformation capture(3C) experiment that measures the frequency of all paired interactions in the entire genome, which is a powerful tool for studying the 3D structure of the genome. The fineness of the constructed genome structure depends on the resolution of Hi-C data. However, due to the fact that high-resolution Hi-C data require deep sequencing and thus high experimental cost, most available Hi-C data are in low-resolution. Hence, it is essential to enhance the quality of Hi-C data by developing the effective computational methods.

Results: In this work, we propose …


3d-Printed Hydrogels Dressings With Bioactive Borate Glass For Continuous Hydration And Treatment Of Second-Degree Burns, Fateme Fayyazbakhsh, Michael J. Khayat, Candy Sadler, Delbert Day, Yue-Wern Huang, Ming-Chuan Leu Jan 2023

3d-Printed Hydrogels Dressings With Bioactive Borate Glass For Continuous Hydration And Treatment Of Second-Degree Burns, Fateme Fayyazbakhsh, Michael J. Khayat, Candy Sadler, Delbert Day, Yue-Wern Huang, Ming-Chuan Leu

Biological Sciences Faculty Research & Creative Works

Recent advances in additive manufacturing have led to the development of innovative solutions for tissue regeneration. Hydrogel materials have gained significant attention for burn wound treatment in clinical practice among various advanced dressings due to their soothing and moisturizing activity. However, prolonged healing, pain, and traumatic removal due to the lack of long-term wound hydration are some of the challenges in the treatment of second-degree burn wounds. In this study, 3D-printed dressings were fabricated using gelatin, alginate, and bioactive borate glass (BBG) using an extrusion-based bioprinter. After ionic crosslinking, the 3D-printed dressings were characterized for mechanical properties, degradation rate, hydration …


Telp Theory: Elucidating The Major Observations Of Rieger Et Al. 2021 In Mitochondria, James Weifu Lee Jan 2023

Telp Theory: Elucidating The Major Observations Of Rieger Et Al. 2021 In Mitochondria, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

The transmembrane-electrostatically localized protons (TELP) theory may represent a complementary development to Mitchell's chemiosmotic theory. The combination of the two together can now excellently explain the energetics in mitochondria. Our calculated transmembrane-attractive force between an excess proton and an excess hydroxide explains how TELP may stay within a 1-nm thin layer at the liquid-membrane interface. Consequently, any pH sensor (sEcGFP) located at least 2–3 nm away from the membrane surface will not be able to see TELP. This feature as predicted from the TELP model was observed exactly in the experiment of Rieger et al., 2021. In contrast to their …


Polymer-Based Microfluidic Device For On-Chip Counter-Diffusive Crystallization And In Situ X-Ray Crystallography At Room Temperature, Sarthak Saha, Can Özden, Alfred Samkutty, Silvia Russi, Aina Cohen, Margaret M. Stratton, Sarah L. Perry Jan 2023

Polymer-Based Microfluidic Device For On-Chip Counter-Diffusive Crystallization And In Situ X-Ray Crystallography At Room Temperature, Sarthak Saha, Can Özden, Alfred Samkutty, Silvia Russi, Aina Cohen, Margaret M. Stratton, Sarah L. Perry

Chemical Engineering Faculty Publication Series

Proteins are long chains of amino acid residues that perform a myriad of functions in living organisms, including enzymatic reactions, signalling, and maintaining structural integrity. Protein function is determined directly by the protein structure. X-ray crystallography is the primary technique for determining the 3D structure of proteins, and facilitates understanding the effects of protein structure on function. The first step towards structure determination is crystallizing the protein of interest. We have developed a centrifugally-actuated microfluidic device that incorporates the fluid handling and metering necessary for protein crystallization. Liquid handling takes advantage of surface forces to control fluid flow and enable …


Exposure To Pcb126 During The Nursing Period Reversibly Impacts Early-Life Glucose Tolerance, Brittany B. Rice, Keegan W. Sammons, Sara Y. Ngo Tenlep, Madeline T. Weltzer, Leryn J. Reynolds, Cetewayo S. Rashid, Hollie I. Swanson, Kevin J. Pearson Jan 2023

Exposure To Pcb126 During The Nursing Period Reversibly Impacts Early-Life Glucose Tolerance, Brittany B. Rice, Keegan W. Sammons, Sara Y. Ngo Tenlep, Madeline T. Weltzer, Leryn J. Reynolds, Cetewayo S. Rashid, Hollie I. Swanson, Kevin J. Pearson

Human Movement Sciences & Special Education Faculty Publications

Polychlorinated biphenyls (PCBs) are persistent environmental organic pollutants known to have detrimental health effects. Using a mouse model, we previously demonstrated that PCB126 exposure before and during pregnancy and throughout the perinatal period adversely affected offspring glucose tolerance and/or body composition profiles. The purpose of this study was to investigate the glucose tolerance and body composition of offspring born to dams exposed to PCB126 during the nursing period only. Female ICR mice were bred, and half of the dams were exposed to either vehicle (safflower oil) or 1 µmole PCB126 per kg of body weight via oral gavage on postnatal …