Engineering Commons^™

N-Glycan Modification In Aspergillus Species, Elke Kainz, Andreas Gallmetzer, Christian Hatzl, Juergen H. Nett, Huijuan Li, Thorsten Schinko, Robert Pachlinger, Harald Berger, Yazmid Reyes-Dominguez, Andreas Bernreiter, Tillmann Gerngross, Stefan Wildt, Joseph Strauss

Dartmouth Scholarship

The production by filamentous fungi of therapeutic glycoproteins intended for use in mammals is held back by the inherent difference in protein N-glycosylation and by the inability of the fungal cell to modify proteins with mammalian glycosylation structures. Here, we report protein N-glycan engineering in two Aspergillus species. We functionally expressed in the fungal hosts heterologous chimeric fusion proteins containing different localization peptides and catalytic domains. . This strategy allowed the isolation of a strain with a functional -1,2-mannosidase producing increased amounts of N-glycans of the Man 5 GlcNAc 2 type. This strain was further engineered by the introduction of …

Spatial Distributions Of Heating, Cooling, And Industrial Degree-Days In Turkey, Ilhami Yildiz, B. Sosaoglu

BioResource and Agricultural Engineering

The degree-day method is commonly used to estimate energy consumption for heating and cooling in residential, commercial and industrial buildings, as well as in greenhouses, livestock facilities, storage facilities and warehouses. This article presents monthly and yearly averages and spatial distributions of heating, cooling, and industrial degree-days at the base temperatures of 18 °C and 20 °C, 18 °C and 24 °C, and 7 °C and 13 °C, respectively; as well as the corresponding number of days in Turkey. The findings presented here will facilitate the estimation of heating and cooling energy consumption for any residential, commercial and industrial buildings …

Biomass And Biofuels In Maine: Estimating Supplies For Expanding The Forest Products Industry, Jonathan Rubin, Kate Dickerson, Jacob Kavkewitz

Energy & the Environment

This paper estimates the renewable energy potential of Maine’s forest resources, and how much energy these resources could potentially provide the state. Using the most recent state-specific data available, and a methodology similar to the Billion Tons Report, we find that ethanol production from Maine’s forest residues could potentially provide 18% of Maine’s transportation (gasoline) fuels with a fermentation wood to ethanol process. Making Fischer-Tropsch diesel (F-T diesel) using forest residues can replace 39% of Maine’s petro-diesel consumption. Actual levels of biofuels that can be produced will depend on conversion factors and forestry residue removals that are subject to uncertainty.

2007 Scholars And Artists Bibliography, Michael Schwartz Library, Cleveland State University, Friends Of The Michael Schwartz Library, Joanne E. Goodell Ph.D.

Scholars and Artists Bibliographies

This bibliography was created for the annual Friends of the Michael Schwartz Library Scholars and Artists Reception, recognizing scholarly and creative achievements of Cleveland State University faculty, staff and emeriti. Dr. Joanne Goodell was the guest speaker.

Are There Rearrangement Hotspots In The Human Genome?, Max A. Alekseyev, Pavel A. Pevzner

Faculty Publications

In a landmark paper, Nadeau and Taylor [18] formulated the random breakage model (RBM) of chromosome evolution that postulates that there are no rearrangement hotspots in the human genome. In the next two decades, numerous studies with progressively increasing levels of resolution made RBM the de facto theory of chromosome evolution. Despite the fact that RBM had prophetic prediction power, it was recently refuted by Pevzner and Tesler [4], who introduced the fragile breakage model (FBM), postulating that the human genome is a mosaic of solid regions (with low propensity for rearrangements) and fragile regions (rearrangement hotspots). However, the rebuttal …

Inference Of Genetic Regulatory Networks With Recurrent Neural Network Models Using Particle Swarm Optimization, Rui Xu, Donald C. Wunsch, Ronald L. Frank

Electrical and Computer Engineering Faculty Research & Creative Works

Genetic regulatory network inference is critically important for revealing fundamental cellular processes, investigating gene functions, and understanding their relations. The availability of time series gene expression data makes it possible to investigate the gene activities of whole genomes, rather than those of only a pair of genes or among several genes. However, current computational methods do not sufficiently consider the temporal behavior of this type of data and lack the capability to capture the complex nonlinear system dynamics. We propose a recurrent neural network (RNN) and particle swarm optimization (PSO) approach to infer genetic regulatory networks from time series gene …

Bactericidal Effects Of Cold Plasma Technology On Geobacillus Stearothermophilus And Bacillus Cereus Microorganisms, Angela D. Morris, Gayle B. Mccombs, Susan L. Tolle, Mounir Laroussi, Wayne L. Hynes

Dental Hygiene Faculty Publications

Cold plasma is a state of matter that contains a large number of particles that are electrically charged. Plasmas generate chemically reactive species and ultraviolet radiation making them useful in decontamination applications (Kong & Laroussi, 2003). Research regarding the inactivation of gram-positive bacteria by cold plasma has been studied by Laroussi et al (2003); however, there is limited research regarding the germicidal effectiveness of cold plasma on Geobacillus stearothermophilus and Bacillus cereus microorganisms. The purpose of this study was to determine if cold plasma technology inactivates Geobacillus stearothermophilus and Bacillus cereus vegetative cells and spores. This study consisted of 981 …

America Competes Act, United States

Ethics in Science and Engineering National Clearinghouse

The full text of the America COMPETES Act. See Sec. 7008. Postdoctoral Research Fellows, and Sec. 7009. Responsible Conduct of Research.

Sheep Updates 2007 - Part 2, Ian Mcfarland, Catherine Stockman, Anne Barnes, David Pethick, Jenny Davis, Brett Thompson, Ron Leng, Sally Pieruzzini, Elizabeth Jackson, Mohammed Quaddus, Nazrul Islam, John Stanton, Melanie Dowling

Sheep Updates

This session covers six papers from different authors:

CONCURRENT SESSIONS

FINISHING LAMB AND BEEF

1. Precision Feedlot Lamb, Ian McFarland, Department of Agriculture and Food, Western Australia

2. Feeding sheep under high heat load did not decrease intake of feedlot rations, Catherine Stockman, Department of Agriculture and Food, Western Australia & Murdoch University, Anne Barnes, Murdoch University David Pethick, Murdoch University

3. Taking the stress out of fifishing lambs and cattle - EasyFeed solutions, Jenny Davis, Brett Thomson, Milne AgriGroup, Welshpool WA, Ron Leng, Emeritus Professor, University of New England, Armidale, NSW

WOOL

4. DAFWA …

Sheep Updates 2007 - Part 4, Dean Thomas, Dominique Blache, Dean Revell, Hayley Norman, Phil Vercoe, Zoey Durmic, Serina Digby, Di Mayberry, Megan Chadwick, Martin Sillence, David Masters, R. Silberstein, F. Byrne, P. G. H. Nichols, J. Young, L. Aitkins, M. G. Wilmot, A. J. Rintoul, T. Lambert, D. R. Mcclements, P. Raper, P. Ward, C. Walton, T. York, David Pethick, David Hopkins, Malcolm Mcphee, D. B. Savage, J. V. Nolan, I. R. Godwin, A. Aoetpah, T. Nguyen, N. Baillie, C. Lawler, Ken Geenty, John Smith, Darryl Smith, Tim Dyall, Grant Uphill, John Young, Michael Paton, John Dorrian

Sheep Updates

This session covers eight papers from different authors:

GRAZING

1. The impact of high dietary salt and its implications for the management of livestock grazing saline land, Dean Thomas, Dominique Blache, Dean Revell, Hayley Norman, Phil Vercoe, Zoey Durmic, Serina Digby, Di Mayberry, Megan Chadwick, Martin Sillence and David Masters, CRC for Plant-based Management of Dryland Salinity, Faculty of Natural & Agricultural Sciences, The University of Western Australia, WA.

2. Sustainable Grazing on Saline Lands - outcomes from the WA1 research project, H.C. Norman^1,2, D.G. Masters^1,2, R. Silberstein^1,2, F. Byrne^2,3, P.G.H. Nichols …

Development And Analysis Of A Software Package To Quantify In Vivo Polyethylene Wear After Total Hip Arthroplasty, Allison Kinney, Catherine G. Ambrose

Mechanical and Aerospace Engineering Faculty Publications

Since the first total hip arthroplasty (THA) in 1938, THA evolved and developed into one of the major concentrations of orthopaedic research. The typical hip implant device used today incorporates a femoral and an acetabular component that serve to replicate the anatomical and mechanical functions of the natural hip joint. However, several problems exist that can effect the function of the implant device. Wear in the polyethylene liner of the acetabular component of the total hip replacement device is known as one of the major factors that affects the longevity of total hip replacement devices. Both manual and computer-aided techniques …

Fabrication Of Ankle-Foot Orthoses Using Selective Laser Sintering Technology, Allison Kinney, M. C. Faustini, Richard R. Neptune, R. H. Crawford, S. J. Stanhope

Mechanical and Aerospace Engineering Faculty Publications

Passive dynamic ankle-foot orthoses (AFOs) are often prescribed to improve gait performance for those with various neuromuscular disorders. Designs and materials used for AFOs range from simple polypropylene braces to advanced custom carbon fiber dynamic AFOs that passively store and release mechanical energy during gait. AFO designs vary in the shape and length of the foot component as well as the stiffness and length of the tibial component, depending on the desired functional outcomes. However, the current fabrication technology is not ideally suited for refined customization of AFO characteristics to optimize performance, or for rapid lowcost, high volume manufacturing and …

A Hybrid First Year Science Course For Engineering Students – Integrating Biology With Chemistry, David Harding, Pauline Schwartz, Jean Nocito-Gobel, Agamemnon Koutsospyros

Civil Engineering Faculty Publications

Biology is playing an increasingly important role in many engineering fields. With the typical engineering program already having a high credit hour requirement, the question becomes, how to best integrate biology concepts into a packed engineering curriculum. A typical biology course is not likely to introduce the important concepts of biology to engineering students. The solution here is to develop a hybrid course that integrates chemistry and biology. In the course, Chemistry with Applications to Biosystems, the concept is to develop a course that integrally links important concepts of chemistry and biology. The course focuses on the areas of biology …

Ethics Across The Curriculum Modules For Eac Toolkit Workshops, William J. Frey, Jose A. Cruz-Cruz

Ethics in Science and Engineering National Clearinghouse

This collection of modules has been designed to show how the Ethics Across the Curriculum Toolkit project provides for the preparation and pairing of student and instructor modules. These modules have been brought together and paired to aid in Toolkit demonstrations designed for faculty developement workshops in ethics across the curriculum.

Abstract by author.

Corn Stover Availability And Collection Efficiency Using Typical Hay Equipment, Robert M. Prewitt, Michael D. Montross, Scott A. Shearer, Timothy S. Stombaugh, Stephen F. Higgins, Samuel G. Mcneill, Shahab Sokhansanj

Biosystems and Agricultural Engineering Faculty Publications

Corn stover has been identified as a potential feedstock for the production of fermentable sugars and thermochemical processes. The availability and efficiency of typical hay equipment for collecting corn stover has not been well quantified. Corn stover was collected for two years on a central Kentucky farm near Louisville. Six different harvesting treatments, using traditional hay equipment, were used to harvest corn stover. A rotary mower, rotary scythe (flail-type mower with windrow-forming shields), parallel bar rake, and a round baler were utilized. The average stover moisture content prior to grain harvest was above 40%, and field drying was required before …

Functional Dissociation In Frontal And Striatal Areas For Processing Of Positive And Negative Reward Information, Xun Liu, David K. Powell, Hongbin Wang, Brian T. Gold, Christine R. Corbly, Jane E. Joseph

Neuroscience Faculty Publications

Reward-seeking behavior depends critically on processing of positive and negative information at various stages such as reward anticipation, outcome monitoring, and choice evaluation. Behavioral and neuropsychological evidence suggests that processing of positive (e.g., gain) and negative (e.g., loss) reward information may be dissociable and individually disrupted. However, it remains uncertain whether different stages of reward processing share certain neural circuitry in frontal and striatal areas, and whether distinct but interactive systems in these areas are recruited for positive and negative reward processing. To explore these issues, we used a monetary decision-making task to investigate the roles of frontal and striatal …

Ethics Bowl Competition As Capstone Activity For Practical And Professional Ethics Classes, William J. Frey, Jose A. Cruz-Cruz

Ethics in Science and Engineering National Clearinghouse

In Connexions, a course is both a course (what is offered in a school curriculum) and a collection of modules. This course as a collection of modules has been designed to pull together the modules published in Connexions by this author for the purpose of showing different aspects of the Ethics Bowl competition and how it can be used in a university course on practical and professional ethics. The Ethics Bowl concept comes from Robert Ladenson of the Illinois Institute of Technology. Through the Association of Practical and Professional Ethics, the Ethics Bowl competition has been carried out nationally for …

Ua66 2007 Student Awards Ceremony, Wku Ogden College Of Science & Engineering

WKU Archives Records

Program recognizing Ogden College students with brief list of activities for each student.

Using Case Studies In Teaching Research Ethics, Kenneth D. Pimple

Ethics in Science and Engineering National Clearinghouse

No abstract provided.

Growth Of Mc3t3-El Pre-Osteoblasts On Borate Containing Glasses & Growth Of Osteoblastic Cells On 13-93 Glass Scaffolds, Agatha Dwilewicz

Opportunities for Undergraduate Research Experience Program (OURE)

Three borate-based glasses, compositions designated 1B, 2B, and 3B, with B₂0₃ levels of 15, 31, and 46 mole percent (Table 1), respectively, were tested for their effects on the growth of MC3T3-El mouse pre-osteoblastic cells under physiological fluid conditions. Silicate type 45S5 glass was used as a control for comparison. Independently, the biocompatibility of recently developed silicate-based porous 13-93 bioactive glass scaffolds was tested; Saos-2 human osteosarcoma cell line was used. Tests performed for the two studies included: contact assays of cell growth at the glass interface, chemical analysis of borate released into the culture medium, quantitative …

Characterization Of Dairy Milk House Waste Water In Kentucky, Anshu Singh, Czarena L. Crofcheck, Gail M. Brion

Biosystems and Agricultural Engineering Faculty Publications

This study focuses on characterization of milk house waste water from eight different farms in Kentucky. The farms were separated into three groups based on the number of cows: small (20-30), medium (30-60), and large (over 60 cows). Samples were collected once a month from four farms and twice a month from the remainder. Samples were analyzed for chemical, biochemical, and microbiological characteristics. Results indicated a large and significant variation in the chemical and microbiological characteristics between the farms. Farm size had a significant effect on the nutrient content of the waste water. Though samples exhibited seasonal variation, there was …

Intellectual Property Management In Health And Agricultural Innovation: A Handbook Of Best Practices, Vol. 2, Anatole Krattiger, Richard T. Mahoney, Lita Nelsen, Jennifer A. Thomson, Alan B. Bennett, Kanikaram Satyanarayana, Gregory D. Graff, Carlos Fernandez, Stanley Kowalski

Law Faculty Scholarship

Prepared by and for policy-makers, leaders of public sector research establishments, technology transfer professionals, licensing executives, and scientists, this online resource offers up-to-date information and strategies for utilizing the power of both intellectual property and the public domain. Emphasis is placed on advancing innovation in health and agriculture, though many of the principles outlined here are broadly applicable across technology fields. Eschewing ideological debates and general proclamations, the authors always keep their eye on the practical side of IP management. The site is based on a comprehensive Handbook and Executive Guide that provide substantive discussions and analysis of the opportunities …

Resistance Of The Target Islet Tissue To Autoimmune Destruction Contributes To Genetic Susceptibility In Type 1 Diabetes., Natasha J. Hill, Aleksandr Stotland, Michelle Solomon, Patrick Secrest, Elizabeth Getzoff, Nora Sarvetnick

Journal Articles: Regenerative Medicine

Type 1 diabetes occurs when self-reactive T lymphocytes destroy the insulin-producing islet beta cells of the pancreas. The defects causing this disease have often been assumed to occur exclusively in the immune system. We present evidence that genetic variation at the Idd9 diabetes susceptibility locus determines the resilience of the targets of autoimmunity, the islets, to destruction. Susceptible islets exhibit hyper-responsiveness to inflammatory cytokines resulting in enhanced cell death and increased expression of the death receptor Fas. Fas upregulation in beta cells is mediated by TNFR2, and colocalization of TNFR2 with the adaptor TRAF2 in NOD beta cells is altered. …

Stream Ambient Noise, Spectrum And Propagation Of Sounds In The Goby Padogobius Martensii: Sound Pressure And Particle Velocity, Marco Lugli, Michael L. Fine

Biology Publications

The most sensitive hearing and peak frequencies of courtship calls of the stream goby, Padogobius martensii, fall within a quiet window at around 100Hz in the ambient noise spectrum. Acoustic pressure was previously measured although Padogobius likely responds to particle motion. In this study a combination pressure (p) and particle velocity (u) detector was utilized to describe ambient noise of the habitat, the characteristics of the goby’s sounds and their attenuation with distance. The ambient noise (AN) spectrum is generally similar for p and u (including the quiet window at noisy locations), although the energy distribution of uspectrum is …

Improved Parameterization To Invert Rayleigh-Wave Data For Shallow Profiles Containing Stiff Inclusions, Carlos Calderon-Macias, Barbara Luke

Civil and Environmental Engineering and Construction Faculty Research

Inversion of shear-wave velocity profiles from phase-velocity measurements of Rayleigh-wave energy for sites containing stiff layers can be erroneous if such layers are not characterized in the starting or reference model. Incorporation of a priori knowledge then is key for converging upon a realistic or meaningful solution. Resolving soil profiles in desert regions where stiff layers cemented with calcium carbonate are intermixed with softer, uncemented media is an application for which locating shallow stiff inclusions has important implications. Identification of the stiff layers is critical for foundation design and cost estimating of excavations. A parameterization that seems adequate for this …

Bioelectric Effects Of Intense Nanosecond Pulses, Karl H. Schoenbach, Barbara Y. Hargrave, Ravindra P. Joshi, Juergen F. Kolb, Richard Nuccitelli, Christopher J. Osgood, Andrei G. Pakhomov, Michael W. Stacey, James R. Swanson, Jody A. White, Shu Xiao, Jue Zhang, Stephen J. Beebe, Peter F. Blackmore, E. Stephen Buescher

Bioelectrics Publications

Electrical models for biological cells predict that reducing the duration of applied electrical pulses to values below the charging time of the outer cell membrane (which is on the order of 100 ns for mammalian cells) causes a strong increase in the probability of electric field interactions with intracellular structures due to displacement currents. For electric field amplitudes exceeding MV/m, such pulses are also expected to allow access to the cell interior through conduction currents flowing through the permeabilized plasma membrane. In both cases, limiting the duration of the electrical pulses to nanoseconds ensures only nonthermal interactions of the electric …

Biotechnology, Life Sciences And Control Engineering:Some Synthesis Issues, Aidan O'Dwyer

Conference papers

The synthesis of biotechnology, life sciences and control engineering is receiving increasing interest. The purpose of this contribution (and the associated poster) is to raise awareness of some of this synthesis work.

Intellectual Property Management In Health And Agricultural Innovation: Executive Guide, Anatole Krattiger, Richard T. Mahoney, Lita Nelsen, Jennifer A. Thomson, Alan B. Bennett, Kanikaram Satyanarayana, Gregory D. Graff, Carlos Fernandez, Stanley Kowalski

Law Faculty Scholarship

Prepared by and for policy-makers, leaders of public sector research establishments, technology transfer professionals, licensing executives, and scientists, this online resource offers up-to-date information and strategies for utilizing the power of both intellectual property and the public domain. Emphasis is placed on advancing innovation in health and agriculture, though many of the principles outlined here are broadly applicable across technology fields. Eschewing ideological debates and general proclamations, the authors always keep their eye on the practical side of IP management. The site is based on a comprehensive Handbook and Executive Guide that provide substantive discussions and analysis of the opportunities …

Intellectual Property Management In Health And Agricultural Innovation: A Handbook Of Best Practices, Vol. 1, Anatole Krattiger, Richard T. Mahoney, Lita Nelsen, Jennifer A. Thomson, Alan B. Bennett, Kanikaram Satyanarayana, Gregory D. Graff, Carlos Fernandez, Stanley Kowalski

Law Faculty Scholarship

Prepared by and for policy-makers, leaders of public sector research establishments, technology transfer professionals, licensing executives, and scientists, this online resource offers up-to-date information and strategies for utilizing the power of both intellectual property and the public domain. Emphasis is placed on advancing innovation in health and agriculture, though many of the principles outlined here are broadly applicable across technology fields. Eschewing ideological debates and general proclamations, the authors always keep their eye on the practical side of IP management. The site is based on a comprehensive Handbook and Executive Guide that provide substantive discussions and analysis of the opportunities …

Rational Risk/Benefit Analysis Of Genetically Modified Crops, Stanley P. Kowalski

Law Faculty Scholarship

Safety concerns over the use of molecular biotechnology in the improvement of crops has generated substantial, heated and confusing debates, often driven by ideology and hysterics. Modification of crops is not new, and biotechnology (in its broadest sense) has been used for over a century to accelerate the development of new crops for food, feed and fibre, so as to meet the demands of a growing global community. The introduction of crops developed via molecular biotechnology [Genetically Modified Crops (GMCs)] represents the latest step in this inexorable innovative progression of technology. However, misinformed concern has led to a broad embrace …