Engineering Commons^™

Monosynaptic Functional Connectivity In Cerebral Cortex During Wakefulness And Under Graded Levels Of Anesthesia, Jeannette A. Vizuete, Siveshigan Pillay, Kamran Diba, Kristina M. Ropella, Anthony G. Hudetz

Biomedical Engineering Faculty Research and Publications

The balance between excitation and inhibition is considered to be of significant importance for neural computation and cognitive function. Excitatory and inhibitory functional connectivity in intact cortical neuronal networks in wakefulness and graded levels of anesthesia has not been systematically investigated. We compared monosynaptic excitatory and inhibitory spike transmission probabilities using pairwise cross-correlogram (CCG) analysis. Spikes were measured at 64 sites in the visual cortex of rats with chronically implanted microelectrode arrays during wakefulness and three levels of anesthesia produced by desflurane. Anesthesia decreased the number of active units, the number of functional connections, and the strength of excitatory connections. …

Computational Model Of Neuron-Astrocyte Interputational Model Of Neuron-Astrocyte Interactions During Focal Seizure Generationactions During Focal Seizure Generation, Davide Reato, Mario Cammarota, Lucas C. Parra, Giorgio Carmignoto

Publications and Research

Empirical research in the last decade revealed that astrocytes can respond to neurotransmitters with Ca2+ elevations and generate feedback signals to neurons which modulate synaptic transmission and neuronal excitability. This discovery changed our basic understanding of brain function and provided new perspectives for how astrocytes can participate not only to information processing, but also to the genesis of brain disorders, such as epilepsy. Epilepsy is a neurological disorder characterized by recurrent seizures that can arise focally at restricted areas and propagate throughout the brain. Studies in brain slice models suggest that astrocytes contribute to epileptiform activity by increasing neuronal excitability …

An Indigoidine Biosynthetic Gene Cluster From Streptomyces Chromofuscus Atcc 49982 Contains An Unusual Indb Homologue, Dayu Yu, Fuchao Xu, Jonathan Valiente, Siyuan Wang, Jixun Zhan

Biological Engineering Faculty Publications

A putative indigoidine biosynthetic gene cluster was located in the genome of Streptomyces chromofuscus ATCC 49982. The silent 9.4-kb gene cluster consists of five open reading frames, named orf1, Sc-indC, Sc-indA, Sc-indB, and orf2, respectively. Sc-IndC was functionally characterized as an indigoidine synthase through heterologous expression of the enzyme in both Streptomyces coelicolor CH999 and Escherichia coli BAP1. The yield of indigoidine in E. coli BAP1 reached 2.78 g/l under the optimized conditions. The predicted protein product of Sc-indB is unusual and much larger than any other reported IndB-like protein. The N-terminal portion of this enzyme resembles IdgB and the …

Toe Brace Designs, Tarun Goswami, Allison L. Van Horn, Alexander O. Sheets

Biomedical, Industrial & Human Factors Engineering Faculty Publications

In various embodiments, provided are braces for use in supporting the metatarophalangeal joint, reducing or maintaining the intermetatarsal angle, enhancing or maintaining alignment of the hallux, or combinations thereof in a subject having hallux valgus.

An Information Architecture For Icme, William Marsden

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

Multiscale Modeling For Material Design Of Solid Oxide Fuel Cell Electrode, Y. Umeno, S. Hara, T. Tada, N. Shikazono

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

Some (Other) Uses Of Diffusion Couples In Metallurgy: Model Calibration And Mapping Transitions In Mechanisms, Christopher Hutchinson

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

From Genome To Flying Cyberalloys: The First Half Century, G. B. Olson

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

A Hybrid Computational-Experimental Approach For Automated Crystal Structure Solution, Bryce Meredig, Scott Kirklin, Chris Wolverton

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

Predicting Microstructure-Property Relationships In Structural Materials Via Multiscale Models Validated By In-Situ Synchrotron Observation, Peter D. Lee, Lang Yuan, Chedtha Puncreobutr, S. Karagadde

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

Nist Databases For Materials Research, T. Vanderah, Vicky Karen, Peter Linstrom, Donald Burgess

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

The Materials Project – A Public Materials Design Platform, Kristin Persson

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

New Stage Of Matnavi, Materials Database At Nims, Toshio Ogata, Masayoshi Yamazaki

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

Method And Apparatus For Utilizing Amplitude-Modulated Pulse-Width Modulation Signals For Neurostimulation And Treatment Of Neurological Disorders Using Electrical Stimulation, Jeffrey Hargrove, Theodore Stokes, Mary L. Esty

Mechanical Engineering Patents

A computing device-controlled system is described for the generation of amplitude-modulated pulse-width modulation (AMPWM) signals for use in treating neurological dysfunction via cranial neurostimulation, where the AMPWM signal is specifically designed to minimize the electrical impedance of the tissues of the head. A low-frequency carrier signal is determined for the AMPWM signal by measuring EEG activity at a reference site or sites, generally corresponding with the location of suspected brain dysfunction. Carrier signal frequency is variably related to critical frequency components of the EEG power spectral density, determined from statistical analysis of amplitudes and variability, and dynamically changed as a …

Designing For Success: Developing Engineers Who Consider Universal Design Principles, Kimberly Edginton Bigelow

Mechanical and Aerospace Engineering Faculty Publications

Engineers must design for a diverse group of potential users of their products; however, engineering curricula rarely include an emphasis on universal design principles. This research article details the effectiveness of a design project implemented in a first-year engineering course in an effort to raise awareness of the need for engineers to be more inclusive when designing. Students were asked to apply universal design principles to redesign an engineering laboratory to make it more usable to all, including individuals with disabilities who use the room. A representative from the university’s disability services staff, as well as individuals with first-hand experience …

Materials Design Based On Ab Initio Thermodynamics, Jorg Neugebauer, Fritz Kormann, Blazej Grabowski, Tilmann Hickel, Dierk Raabe

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

Materials Property Microscopy Tools For The Materials Genome Initiative, J. C. Zhao, David Cahill

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

High-Throughput Synthesis And Analysis For Searching New Permanent Magnet Materials, Gerhard Schneider, Dagmar Goll, Ralf Loffller, Roman Karimi

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

A Software Framework For Designing Material, David Cebon, Mike Ashby

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

Rapid Temporal Changes In The Expression Of A Set Of Neuromodulatory Genes During Alcohol Withdrawal In The Dorsal Vagal Complex: Molecular Evidence Of Homeostatic Disturbance., Kate Freeman, Mary M. Staehle, Zeynep H Gümüş, Rajanikanth Vadigepalli, Gregory E Gonye, Carmen N Nichols, Babatunde A Ogunnaike, Jan B Hoek, James S Schwaber

College of Science & Mathematics Departmental Research

BACKGROUND: Chronic alcohol exposure produces neuroadaptation, which increases the risk of cellular excitotoxicity and autonomic dysfunction during withdrawal. The temporal progression and regulation of the gene expression that contributes to this physiologic and behavioral phenotype is poorly understood early in the withdrawal period. Further, it is unexplored in the dorsal vagal complex (DVC), a brainstem autonomic regulatory structure.

METHODS: We use a quantitative polymerase chain reaction platform to precisely and simultaneously measure the expression of 145 neuromodulatory genes in more than 100 rat DVC samples from control, chronically alcohol-exposed, and withdrawn rats. To gain insight into the dynamic progression and …

Pedaling-Related Brain Activation In People Post-Stroke: An Fmri Study, Nutta-On Promjunyakul

Dissertations (1934 -)

This study aimed to enhance our understanding of supraspinal control of locomotion in stroke survivors and its relationship to locomotor impairment. We focused mainly on the locomotor component of walking, which involves rhythmic, reciprocal, flexion and extension movements of multiple joints in both legs. Functional magnetic resonance imaging (fMRI) was used to record human brain activity while pedaling was used as a model of locomotion. First, we examined the spatiotemporal characteristics of hemodynamic responses recorded with fMRI and found that they were different in stroke survivors and control subjects. However, these differences were not substantial enough to require altering the …

Quantitation And Identification Of Protein S-Nitrosylation: Implication For The Progression Of Alzheimer's Disease, Siyang Wang

Doctoral Dissertations

Protein S-nitrosylation, the covalent modification of a cysteine sulfhydryl group by nitric oxide (NO), plays a critical role in post-translational modification (PTM) that regulates a large variety of cellular functions and signalling events. The nitrosylation state changes with oxidative damage and is involved in variety of cancers and neurodegeneration diseases.

Three technologies were developed for nitrosylated protein detection and identification. Capillary gel electrophoresis with laser induced fluorescence (CGE-LIF) detection was used for the detection and quantitation of nitrosylated proteins. A two-dimensional micro-electrophoresis (2D μ-CE) separations system was also built to detect nitrosylated proteins via poly (methylmethacrylate) microchips. Proteomics following nitrosylated …

The Contribution Of Oxidative Stress In The Protein Damage And Dna Lesion In Alzheimer's Disease Neuropathology, Cheng Zhang

Doctoral Dissertations

Glutathione (GSH) plays an essential role in the intracellular antioxidant defense against the oxidant radicals, especially the ·OH radical. To understand the early and progressive cellular changes in Alzheimer's disease (AD) development, we investigated reduced glutathione/oxidized glutathione (GSH/GSSG) status in a double mutated AD transgenic mouse model (B6.Cg-Tg), which carries Swedish amyloid precursor protein mutation (APPswe) and exon 9 deletion of the PSEN1 gene. Likewise, S-glutathionylation (Pr-SSG) is a specific post-translational modification (PTM) of cysteine residues by the addition of glutathione. S-glutathionylated proteins induced by oxidative stress play an essential role in understanding the pathogenesis of the aging …

A Novel Microfluidic Enrichment Technique For Carbonylated Proteins, Bryant C. Hollins

Doctoral Dissertations

Proteins are the building blocks of cells in living organisms, and are composed of amino acids. The expression of proteins is regulated by the processes of transcription and translation. Proteins undergo post-translational modifications in order to dictate their role physiologically within a cell.

Not all post-translational modifications are beneficial for the protein or the cell. One type of post-translational modification, called carbonylation, irreversibly places a carbonyl group onto an amino acid residue, most commonly proline, lysine, arginine, and threonine. This modification can have severe consequences physiologically, including loss of solubility, loss of function, and protein aggregation.

Carbonylated proteins have commonly …

Oscillator-Based Neuronal Modeling For Seizure Progression Investigation And Seizure Control Strategy, Wu Chen

Doctoral Dissertations

The coupled oscillator model has previously been used for the simulation of neuronal activities in in vitro rat hippocampal slice seizure data and the evaluation of seizure suppression algorithms. Each model unit can be described as either an oscillator which can generate action potential spike trains without inputs, or a threshold-based unit. With the change of only one parameter, each unit can either be an oscillator or a threshold-based spiking unit. This would eliminate the need for a new set of equations for each type of unit. Previous analysis has suggested that long kernel duration and imbalance of inhibitory feedback …

A Hybrid Brain-Computer Interface Based On Motor Intention And Visual Working Memory, Ching-Chang Kuo

Doctoral Dissertations

Non-invasive electroencephalography (EEG) based brain-computer interface (BCI) is able to provide alternative means for people with disabilities to communicate with and control over external assistive devices. A hybrid BCI is designed and developed for following two types of system (control and monitor).

Our first goal is to create a signal decoding strategy that allows people with limited motor control to have more command over potential prosthetic devices. Eight healthy subjects were recruited to perform visual cues directed reaching tasks. Eye and motion artifacts were identified and removed to ensure that the subjects' visual fixation to the target locations would have …

Real-Time Analysis Of Brain Tumor Cell Dynamics: Novel Thermoelectric Detection Of L-Glutamate And Cell Metabolism Using Microfluidics, Siva Mahesh Tangutooru

Doctoral Dissertations

This study describes the design, fabrication and applications of a novel thermoelectric microfluidic bio-sensor. The bio-sensor is used for real time detection of the L-glutamate (L-glu) dynamics and metabolism for brain tumor cells immobilized in a microfluidic device. The microfluidic device is fabricated using a polymer/glass laminating technique (Xurography). An antimony-bismuth thin-film thermopile (primary sensing element) is integrated to the microfluidic device. The brain tumor cells are immobilized over the thermopile covering measuring and reference junctions of the thermopile using a poly-l-lysine coating layer. L-glutamate oxidase (L-GLOD) is immobilized over the measuring junctions of the thermopile prior to the immobilization …

Atomis'c Materials Design Using The Computa'onal Materials Repository, Karsten W. Jacobsen

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

High Throughput Mapping Of The Thermophysical Properties Of Materials, David G. Cahill, Xuan Zheng, Wen-Pin Hsieh, J. C. Zhao

Harnessing The Materials Genome: Accelerated Materials Development via Computational and Experimental Tools

No abstract provided.

Design And Validation Of Bending Test Method For Characterization Of Miniature Pediatric Cortical Bone Specimens, Carolyne Albert, John Jameson, Gerald F. Harris

Biomedical Engineering Faculty Research and Publications

Osteogenesis imperfecta is a genetic disorder of bone fragility; however, the effects of this disorder on bone material properties are not well understood. No study has yet measured bone material strength in humans with osteogenesis imperfecta. Small bone specimens are often extracted during routine fracture surgeries in children with osteogenesis imperfecta. These specimens could provide valuable insight into the effects of osteogenesis imperfecta on bone material strength; however, their small size poses a challenge to their mechanical characterization. In this study, a validated miniature three-point bending test is described that enables measurement of the flexural material properties of pediatric cortical …