Engineering Commons^™

Thermal Preconditioning Effects On Perforation Propensity Of Transvenous Implantable Cardiac Leads Used For Pacing And Defibrillation, Diane Muff

Master's Theses

Thermal preconditioning to simulate shipping, handling and storage conditions did not affect distal tip stiffness values for permanently implantable cardiac leads used for pacing and defibrillation. Leads that were subjected to extreme temperatures and temperature cycling did not show changes in buckle force values compared with control specimens which were maintained at ambient room conditions. Absolute differences between all measurements were small, generally under 0.05 N and were attributable to measurement variability.

Buckle force values are used to assess the propensity of leads to perforate the heart, a rare but potentially serious complication of implantable pacing and defibrillation systems. Since …

In-Vitro Hydrogel Blood Clots, Ethan Evans, Khoi-Austin Ngo

Bioengineering Senior Theses

Creating a sustainable, ethical, and affordable substitute for animal blood in balloon catheter testing for Deep Vein Thrombosis (DVT) is important to take into consideration. This research utilizes agarose and dextran to develop hydrogel-based artificial blood clots. These hydro gels replicate the mechanical characteristics of human thrombi across acute, subacute, and chronic stages through their elastic and viscous moduli (G' and G") and phase angles. This project addresses extant testing protocols, including temperature regulation, agarose processing, and well plate size, and suggests efficient approaches to encounter these problems.

The hydrogels were characterized by means of a thorough literature review and …

Packed-Bed Reactor For Integrated Peritoneal Dialysis, Julia Appleget, Ally Kwong, Megan Moglia

Bioengineering Senior Theses

Patients with chronic kidney disease (CKD) have lost kidney function. 800,000 Americans have end-stage kidney disease resulting in one of the leading causes of mortality cross-listed with diabetes and heart disease. Over the past decade, the percentage of patients using peritoneal dialysis (PD) has nearly doubled. PD which is a portable treatment involves the insertion of a catheter into the selectively permeable peritoneal cavity infused with a hyperosmolar dialysis fluid drawing the toxins from capillary beds of the peritoneum and into a gravity fed waste solution with a reported clearance of 87%. In order to improve the clearance of the …

Technocene, Vir Joseph Naidu

Masters Theses

Embodied human communication within the Anthropocene. Existing at the intersection of technology, and the body.

The design industry has developed technology that is, paradoxically, isolating. The exposure to a vast audience in the digital sphere has introduced new societal pressures, leading to a disconnection from our immediate surroundings, detached, and donning metaphorical masks. Technocene lives on the fringes of the discipline by blending conceptual thinking with practical application. Through curious, experimental artifacts, it prompts us to shed our masks and embrace vulnerability. Technocene endeavors to reimagine the human experience by acting as a discursive design project. It probes the boundaries …

Advances In Bone And Orthopedics 2024 Edition, Jean-Philippe Berteau, Laurent Pujo-Menjouet

Publications and Research

We are delighted to present the latest edition of ”Advances in Bone and Orthopedics,” a comprehensive compilation of pioneering research and innovative developments in the field. This volume brings together a selection of peer-reviewed abstracts from BONITOS 2024, the Fourth Season of the Bone and Orthopedics Interdisciplinary Symposium, held on May 31st, 2024, at the College of Staten Island in New York. This symposium, a hallmark event in the calendar of professionals dedicated to bone health and orthopedic excellence. The contributions within this volume reflect the breadth and depth of research presented at BONITOS 2024. These abstracts represent cutting-edge studies …

Smart Cushion Device For Elderly Aging In Place, Anna Dion, Nate Colley, Leonardo Bravo

Biomedical Engineering

Our task at hand is to develop a consumer-focused solution for improving elder care to provide additional peace of mind for seniors and caregivers using technology to extend independent living. We intend to create a device that will improve the quality of life of elderly individuals living alone by encouraging periodical movement throughout the day, along with monitoring pressure distribution and alerting when pressure concentration is potentially harmful. It will also alert users to the presence of moisture which can arise from spills or incontinence. The key stakeholders of this project include elderly adults over the age of 65 who …

Aerobic Exercise Improves Depressive Symptoms In The Unilateral 6-Ohda-Lesioned Rat Model Of Parkinson's Disease, Hannah Loughlin, Jacob Jackson, Chloe Looman, Alayna Starll, Jeremy Goldman, Zhiying Shan, Chunxiu Yu

Michigan Tech Publications

Aerobic exercise has been shown to have established benefits on motor function in Parkinson's disease (PD). However, the impact of exercise on depressive symptoms in PD remains unclear. This study aimed to investigate the effects of regular exercise, specifically using a forced running wheel, on both motor performance and the prevalence of depression in a unilateral 6-OHDA-lesioned rat model of PD. The behavioral outcomes of exercise were assessed through the rotarod test (RT), forelimb adjusting step test (FAST), sucrose consumption test (SCT), and novelty sucrose splash test (NSST). Our data revealed evident depressive symptoms in the PD animals, characterized by …

Prosthetic Forearm Controller, Claire Tylutki, Kevin Lopatka, Sofia Zaletel, Hardit Singh

Electrical Engineering

The EMG controlled prosthetic arm project represents a significant advancement in upper limb prosthetics, leveraging cutting-edge technology to address critical user needs. With over two million individuals in the United States living with limb loss, a substantial portion being upper limb amputees, the demand for accessible and functional prosthetic solutions is pressing. This project focuses largely on refining prosthetic limb control through the integration of electromyography (EMG) signal processing and control. Using surface electrodes, this project employs non-invasive means of data collection combined with modern digital signal processing methods to implement faster and more accurate control. The control system also …

Bolin Symes Prosthetic, Samuel C. Collins, Rowan Sun, Jake Hacker

Biomedical Engineering

This project’s purpose is to design a comfortable prosthetic for an individual with a Syme’s amputation, which is a below-knee amputation that removes the foot from the leg at the ankle joint. The goal is to minimize the discomfort that is associated with the current prosthetic leg options for the client, Mr. Bolin, who has the Syme’s amputation. This project also seeks to improve mobility, quality of life, and activity levels of the client. The stakeholder for this project is Mr. Bolin. The final prototype performed successfully when worn by the client and passed comfort testing. Furthermore, COMSOL modeling of …

Foot And Ankle Exoskeleton, Janet M. Velasquez Cabral, Kathryn C. Hamilton, Grant R. Lautze

Biomedical Engineering

This paper presents a non-motorized foot and ankle exoskeleton to assist persons who have no lower limb or abdominal function. Exoskeletons are defined as “an artificial external supporting structure” [1], they are wearable devices that can be used to assist the capabilities of the human body. Although the exoskeleton must be used with crutches, plantarflexion and dorsiflexion will be implemented within the ankle joint to alleviate strain on the foot and ankle, strategically designed in a way that supports the user so their walking performance is as ideal as possible. Additionally, safety straps will be integrated into the foot portion …

Prosthetic Aid For Lifting (Pal), Emma Caringella, Cameron Yartz, Dawn Veditz

Biomedical Engineering

Approximately 1.7 million people in the United States use a limb prosthetic [1]. For these individuals, their prosthetic often enables them to engage in their choice of physical activities. Many prosthetic companies design prosthetic limbs for use in specific athletic activities such as weightlifting. These devices are not designed for everyday wear or a large variety of tasks but enable the person to interact with free weights and workout machines. According to an email conversation between the team and Bob Radocy, Founder of Therapeutic Recreational Services (TRS Inc.) and upper limb amputee, while he could not reveal how many people …

Deep Vein Thrombosis Diagnostic Device, Alisha Mitha, Karina Martel, Ayla Silbahar

Biomedical Engineering

Deep Vein Thrombosis is one of the leading causes of death in hospitals worldwide due its ability to form in patients who are sedentary after receiving care. There is a need for a DVT diagnostic device that can be worn constantly in order to alert medical professionals if a thrombosis is forming, which is the basis of this senior project. The current solutions are time consuming, and look for thrombi after they have fully formed. The goal of this senior project is to design and construct a device to constantly monitor in-hospital patients for early stage DVT and alert hospital …

Accessible Real-Time Eye-Gaze Tracking For Neurocognitive Health Assessments, A Multimodal Web-Based Approach, Daniel C. Tisdale

Master's Theses

We introduce a novel integration of real-time, predictive eye-gaze tracking models into a multimodal dialogue system tailored for remote health assessments. This system is designed to be highly accessible requiring only a conventional webcam for video input along with minimal cursor interaction and utilizes engaging gaze-based tasks that can be performed directly in a web browser. We have crafted dynamic subsystems that capture high-quality data efficiently and maintain quality through instances of user attrition and incomplete calls. Additionally, these subsystems are designed with the foresight to allow for future re-analysis using improved predictive models, as well as enable the creation …

The Wildland Firefighter Respiratory Protection Device, Oliver Pilon, Xaviera Pons, Calder Wood, Tommy Ozawa, Jack Adema, Payton Mayer

Biomedical Engineering

This paper presents the development of First Respire and their design of a powered air-purifying respirator (PAPR) specifically curated for wildland firefighter while simultaneously addressing the critical absence of respiratory protection for wildland firefighters operating in the challenging wildland-urban interface (WUI) environments. Current respiratory protection options, such as bandanas and N95 masks, fall short in providing adequate defense against toxic particulate matter and gases, exposing firefighters to increased risks of health complications, including cancers and respiratory diseases. Through collaborative efforts of the Wildfire Conservancy, the WUI Fire Institute at Cal Poly, and CAL FIRE, First Respire aims to create a …

Liftwalker, Kai A. De La Cruz, Lexie Caterine, Adam Monroe, Jame Whealan

Mechanical Engineering

The LiftWalker’s purpose is to lift users with degenerative diseases from a seated to standing position and support them as they practice the motion of walking as a form of physical therapy. The current LiftWalker is upward of $5,000 so we redesigned the LiftWalker to be more accessible around the world. This meant using affordable and accessible materials and manufacturing processes. To achieve this, we made our design mostly out of bike parts and off-the-shelf components. When it came to the lift system, we found that to maintain a safe and comfortable design it was best to purchase a trailer …

Mechanical Environment Influences Muscle Activity During Infant Rolling, Danielle N. Siegel, Safeer F. Siddicky, Wyatt D. Davis, Erin M. Mannen

Mechanical and Biomedical Engineering Faculty Publications and Presentations

An infant's musculoskeletal and motor development is largely affected by their environment. Understanding how different mechanical environments affect an infant's movements and muscle use is necessary to inform the juvenile products industry and reduce incidents involving inclined nursery products each year. The purpose of this study was to determine how the coordinated movements and corresponding muscle activation patterns are affected by different mechanical environments, specifically the back incline angle. Thirty-eight healthy infants (age: 6.5 ± 0.7 months; 23 M/15 F) were enrolled in this IRB-approved in-vivo biomechanics study. Surface electromyography sensors recorded muscle activity of the erector spinae, abdominal muscles, …

Kinomatic Hip Arthroplasty Pelvic Tilt, Emily Wolfert, Teddy Alberton, Sydney Dresser

Biomedical Engineering

Kinomatic is a startup company that provides custom preoperative surgical plans for orthopedic surgeons. The surgical plans are intended for total hip and knee arthroplasty to provide patient-specific measurements and implant sizing. Surgeons can view the surgical plans as 3D models in virtual reality with an Oculus Quest 2 headset or as a PDF summary. Most patients receive a diagnostic anterior-posterior (AP) x-ray that allows their surgeon to determine if they are eligible for a total hip or knee replacement. After diagnosis, the patient can also choose a Kinomatic surgical plan to provide their surgeon with additional data points before …

2-Channel Eeg Neurofeedback System, Tim Erwin, Donna Nikjou, Sebastian Turkewitz

Electrical Engineering

This work describes the design of an EEG-based neurofeedback system which provides users with real-time feedback on their level of focus or relaxation. By analyzing the spectral content of the brain activity measured via scalp electrodes, focus and relaxation levels can be quantified. Based on these measurements, live feedback in the form of moving bar graphs is provided to users, allowing them to gain awareness of their mental-state and more efficiently learn how to consciously relax or focus. This project covers the design of the system, including amplification and filtering stages, digitization, and signal processing. The system interfaces with a …

Naturify 2300, Yarina Yiwei Dai

Masters Theses

In my art practice, I explore the interplay between human desires to manipulate and anthropomorphize nature, as seen in the technological augmentation of plants and living entities. This investigation delves into how this intersection, alongside empathy towards these creations, contributes to fears of uncontrollability and the risks of addiction and excessive dependence on technology.

Bioengineering and genetic modification have cultivated unprecedented developments, allowing humans to manipulate the fundamental building blocks of life. My research speculates on this technology further, modifying the genetic code of organisms and creating bioengineered wearable entities with enhanced traits or entirely new functionalities. The primary objective …

Architecture As A Carbon-Based Practice, Qixin Yu

Masters Theses

Carbon serves as both a silent protagonist and a looming antagonist in the narrative of architecture, shaping not only the physical structures we inhabit but also the ecological legacy we leave behind.

Centuries of human exploitation of the environment have led to climate and material crises. Shifting this dynamic requires action at micro (matter), meso (material), and macro (materiality) levels. Biogenic materials offer significant potential for carbon sequestration and present opportunities for the building industry to collaborate with nature rather than merely extract from it.

This thesis establishes a research and manufacturing practice that prioritizes material innovation, carbon sequestration, environmental …

Coolsplint: Inflammation Reduction Accessory, Laura C. Denney, Winston H. Giang, Aanchal Singh

Biomedical Engineering

This document outlines a project sponsored by Elizabeth Mentel and Michael Clausson that addresses the shortcomings in current splinting options, particularly for wrist fractures in elderly patients. Stakeholders include clinicians, patients, and emergency medical technicians. Multiple improvements can be made to current splinting options to help alleviate inflammation along with splint versatility, affordability, accessibility, and ease of use. After determining the critical customer requirements, the primary objective of this project was to find a way to manage inflammation. The project explores existing splinting solutions and proposes a new design that utilizes cooling rods to reduce inflammation. The document reviews various …

Recommendation Of Minimal Distal Tibial Length For Long Axis Coordinate System Definitions, Erika P. Muhlrad, Andrew C. Peterson, Abigail M. Anderson, Katelyn C. Aragon, Rich J. Lisonbee, Bruce A. Macwilliams, Karen M. Kruger, Amy L. Lenz

Biomedical Engineering Faculty Research and Publications

Accurate anatomical coordinate systems for the foot and ankle are critical for interpreting their complex biomechanics. The tibial superior-inferior axis is crucial for analyzing joint kinematics, influencing bone motion analysis during gait using CT imaging and biplane fluoroscopy. However, the lack of consensus on how to define the tibial axis has led to variability in research, hindering generalizability. Even as advanced imaging techniques evolve, including biplane fluoroscopy and weightbearing CT, there exist limitations to imaging the entire foot together with the full length of the tibia. These limitations highlight the need to refine axis definitions. This study investigated various superior-inferior …

Cervical Spinal Cord Angiography And Vessel-Selective Perfusion Imaging In The Rat, Seongtaek Lee, Brian Schmit, Shekar N. Kurpad, Matthew D. Budde

Biomedical Engineering Faculty Research and Publications

Arterial spin labeling (ASL) has been widely used to evaluate arterial blood and perfusion dynamics, particularly in the brain, but its application to the spinal cord has been limited. The purpose of this study was to optimize vessel-selective pseudocontinuous arterial spin labeling (pCASL) for angiographic and perfusion imaging of the rat cervical spinal cord. A pCASL preparation module was combined with a train of gradient echoes for dynamic angiography. The effects of the echo train flip angle, label duration, and a Cartesian or radial readout were compared to examine their effects on visualizing the segmental arteries and anterior spinal artery …

Optimizing Recombinant Cas9 Expression: Insights From E. Coli Bl21(De3) Strains For Enhanced Protein Purification And Genome Editing, Shilpi Agrawal, Made Harumi Padmaswari, Abbey L. Stokes, Daniel Maxenberger, Morgan Reese, Adila Khalil, Christopher E. Nelson

Biomedical Engineering Faculty Publications and Presentations

The CRISPR-Cas9 system is a revolutionary tool in genetic engineering, offering unprecedented precision and efficiency in genome editing. Cas9, an enzyme derived from bacteria, is guided by RNA to edit DNA sequences within cells precisely. However, while CRISPR-Cas9 presents notable benefits and encouraging outcomes as a molecular tool and a potential therapeutic agent, the process of producing and purifying recombinant Cas9 protein remains a formidable hurdle. In this study, we systematically investigated the expression of recombinant SpCas9-His in four distinct Escherichia coli (E. coli) strains (Rosetta2, BL21(DE3), BL21(DE3)-pLysS, and BL21(DE3)-Star). Through optimization of culture conditions, including temperature and …

Scaffold-Free Development Of Multicellular Tumor Spheroids With Spatial Characterization Of Structure And Metabolic Radial Profiles, Shelby N. Bess, Gaven K. Smart, Matthew J. Igoe, Timothy J. Muldoon

Biomedical Engineering Faculty Publications and Presentations

Purpose

In vitro assays are essential for studying cellular biology, but traditional monolayer cultures fail to replicate the complex three-dimensional (3D) interactions of cells in living organisms. 3D culture systems offer a more accurate reflection of the cellular microenvironment. However, 3D cultures require robust and unique methods of characterization.

Methods

The goal of this study was to create a 3D spheroid model using cancer cells and macrophages, and to demonstrate a custom image analysis program to assess structural and metabolic changes across spheroid microregions.

Results

Structural characterization shows that cells at the necrotic core show high normalized fluorescence intensities of …

Development Of A Two-Photon Imaging System, Jesseca Hollenbaugh

University Honors Theses

The objective of this project was to convert a Sarastro 2000 confocal laser scanning microscope (CLSM) into a system capable of far-field two-photon excitation (TPE) imaging for the use of the PSU Biology department. TPE microscopy operates on the ability of fluorophores to accept two photons each with half the energy of a desired transition in a single quantum event via a virtual energy state and then emit a higher energy photon upon relaxation. This is preferable to single-photon excitation (SPE) imaging due to lower photon imaging, causing less damage to delicate biological samples, as well as the inherent localization …

Computational Microscopy For Biomedical Imaging With Deep Learning Assisted Image Analysis, Yuwei Liu

Dissertations

Microscopy plays a crucial role across various scientific fields by enabling structural and functional imaging with microscopic resolution. In biomedicine, microscopy contributes to basic research and clinical diagnosis. Conventionally, optical microscopy derives its contrast from the amplitude of the optical wave and provides visualization of the physical structure of the sample qualitatively. To understand the function at the cellular or tissue level, there is a need to characterize the sample quantitatively and explore contrast mechanisms other than light intensity. Image enhancement or reconstruction from microscopic imaging systems is known as computational microscopy, and it involves the application of computational techniques …

Effects Of Exoskeletal-Assisted Walking Rehabilitation On Gait Of Patients Post Stroke, Archisha Kanchan

Theses

Stroke can have detrimental effects on one's body. Its symptoms can range from lightheadedness to blurred vision to body paralysis. One of the more common effects of stroke is difficulty walking where many of the patients who have suffered from stroke are unable to ambulate fully. To mitigate gait difficulties, patients typically follow through with various kinds of physical therapy to help with their gait movements. Recently, robotic exoskeleton (RE) walking therapy has been used as a more innovative approach to gait rehabilitation, in conjunction with other physical therapy session techniques. However, studies involving RE therapy within the stroke population …

Targeted Drug Delivery: Investigating Protein Corona Behavior, Atharva Markale

Theses

Due to high specificity and less toxicity, nanoparticles have become promising for targeted drug delivery against cancer, although they face challenges when they enter the bloodstream, making their behavior unpredictable. The deposition of proteins on nanoparticles, the protein corona, changes their biosignature, affecting their circulation, drug release potency, targeting ability, and immunogenicity. The continuous exchange of protein layers on a nanoscale is complex to analyze.

Nanoparticle tracking analysis (NTA) is a method to characterize nanoparticles in blood plasma by combining principles of light scattering microscopy and Brownian motion. In this work, time-dependent experiments with plasma were conducted to analyze the …

Investigating The Relationship Between Hypoxia, Hypoxia-Inducible Factor 1, And The Optical Redox Ratio In Response To Radiation Therapy, Jesse D. Ivers, Nagavenkatasai Puvvada, Charles M. Quick, Narasimhan Rajaram

Biomedical Engineering Faculty Publications and Presentations

Significance

Radiation resistance is a major contributor to cancer treatment failure and is likely driven by multiple pathways. Multivariate visualization that preserves the spatial co-localization of factors could aid in understanding mechanisms of resistance and identifying biomarkers of response.

Aim

We aim to investigate the spatial and temporal relationship between hypoxia, hypoxia-inducible factor 1 (HIF-1α), and metabolism in response to radiation therapy in two cell lines of known radiation resistance and sensitivity.

Approach

Two-photon excited fluorescence and fluorescence lifetime imaging microscopy were used to quantify the optical redox ratio (ORR) and NAD(P)H fluorescent lifetime and bound fraction in frozen tumor …