Engineering Commons^™

A Mechanistic Model For Internal Bone Remodeling Exhibits Different Dynamic Responses In Disuse And Overload, Scott J. Hazelwood, R. Bruce Martin, Mark M. Rashid, Juan J. Rodrigo

Biomedical Engineering

Bone is a dynamic tissue which, through the process of bone remodeling in the mature skeleton, renews itself during normal function and adapts to mechanical loads. It is, therefore, important to understand the effect of remodeling on the mechanical function of bone, as well as the effect of the inherent time lag in the remodeling process. In this study, we develop a constitutive model for bone remodeling which includes a number of relevant mechanical and biological processes and use this model to address differences in the remodeling behavior as a volume element of bone is placed in disuse or overload. …

Michaelis–Menten Kinetics For Enzymatic Depolymerization Of A Linear Polyester Synthesized From Z-Protected Glutamic Acid, R. M. Tadros, Hossein Noureddini, Delmar C. Timm

Papers in Biomaterials

A biodegradable polyester resin was polymerized from N-benzyloxycarbonyl-L-glutamic acid and ethylene glycol. Rhizopus delemar lipase was used as a biocatalyst for the rupture of ester bonds during the hydrolysis studies. Depoly-merization was observed to followa Michaelis–Menten mechanism, with the maximumrate of monomer formation dP/dt_max = 1.12 x 10^-8 mol/s and the rate constant K_m x 2.03 x 10^-4 mol. Subject to initial conditions described by the most probable distribution and Michaelis–Menten–type depolymerization rate expressions, population density distribution dynamics of the polymeric molecules that formed the resin were explicitly described using a deterministic approach.

Semiautomated Skeletonization Of The Pulmonary Arterial Tree In Micro-Ct Images, Christopher C. Hanger, Steven Thomas Haworth, Robert C. Molthen, Christopher A. Dawson

Biomedical Engineering Faculty Research and Publications

We present a simple and robust approach that utilizes planar images at different angular rotations combined with unfiltered back-projection to locate the central axes of the pulmonary arterial tree. Three-dimensional points are selected interactively by the user. The computer calculates a sub- volume unfiltered back-projection orthogonal to the vector connecting the two points and centered on the first point. Because more x-rays are absorbed at the thickest portion of the vessel, in the unfiltered back-projection, the darkest pixel is assumed to be the center of the vessel. The computer replaces this point with the newly computer-calculated point. A second back-projection …

Pulmonary Arterial Remodeling Revealed By Microfocal X-Ray Tomography, Kelly L. Karau, Robert C. Molthen, Anita H. Dhyani, Steven Thomas Haworth, Christopher A. Dawson

Biomedical Engineering Faculty Research and Publications

Animal models and micro-CT imaging are useful for understanding the functional consequences of, and identifying the genes involved in, the remodeling of vascular structures that accompanies pulmonary vascular disease. Using a micro-CT scanner to image contrast-enhanced arteries in excised lungs from fawn hooded rats (a strain genetically susceptible to hypoxia induced pulmonary hypertension), we found that portions of the pulmonary arterial tree downstream from a given diameter were morphometrically indistinguishable. This 'self-consistency' property provided a means for summarizing the pulmonary arterial tree architecture and mechanical properties using a parameter vector obtained from measurements of the contiguous set of vessel segments …

An Automated Approach For Comparative Analysis Of Interval And Heart Rate Variability, Taral Oza

Theses

Cardiovascular disease is the primary cause of death in the adult population. Half of these cardiac deaths occur as sudden death. Some of the parameters from the surface ECG recording (a noninvasive technique) carry important information and have special significance in research, as they are good indicators of diseases leading to heart attacks, sudden cardiac death and other similar cardiac problems. Measurement of QT interval variability and heart rate variability are two such parameters, which has received much attention of researchers.

An algorithm has been developed to measure and compare the two significant parameters, the QT interval variability and the …

System And Process For Producing Biodiesel Fuel With Reduced Viscosity And A Cloud Point Below Thirty-Two (32) Degrees Fahrenheit , Hossein Noureddini

Papers in Biomaterials

Triglycerides are reacted in a liquid phase reaction with a homogeneous basic catalyst and alcohol, such as ethanol or methanol. The reaction yields a spatially separated two phase result with an upper located non-polar phase consisting principally of transesterified triglycerides and a lower located phase consisting principally of crude glycerol and residual transesterified triglycerides. The transesterified triglycerides and/or glycerol phase is/are optionally, each separately passed through strong cationic ion exchanger(s) to remove anions, resulting in neutral product(s). The crude glycerol is then flashed to remove alcohol and is reacted with an etherifying agent, such as isobutylene or isoamylene, in the …

Manure Impacts On Interrill Erosion, John E. Gilley, Bahman Eghball, J. M. Blumenthal, David D. Baltensperger

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

This study was conducted to measure runoff and erosion from interrill areas as affected by the long-term application of manure and fertilizer to a Tripp sandy loam soil located near Mitchell, Nebraska. Soil that had been removed from the top 0.1 m of the soil profile was placed in a 1 m² soil pan. Rainfall was then applied to the soil pan during initial and wet simulation events. Total runoff was similar on the manure and no-manure treatments. The long-term application of manure (55 years) at a rate of 27 Mg ha^-1 (wet basis) per year did not …

Energy Loss And Stiffness Properties Of Dynamic Elastic Response Prosthetic Feet, Mark Geil

Faculty Articles

No abstract provided.

Application Of Digital Image Processing To Marker-Free Analysis Of Human Gait, Jane Courtney, David Burke, Annraoi Depaor

Articles

The standard method of human gait analysis in use in gait laboratories today invariably involves marker-based motion tracking systems. Although somewhat effective, these methods require accurate placement of awkward external markers. We report on an enhanced approach being researched and developed at the National Rehabilitation Hospital, Dublin based on marker-free motion tracking incorporating advanced digital image processing techniques.

Is Extended Volume Of External Beam Irradiation Beneficial In Post-Esophagectomy High Risk Patients Receiving Combined Chemoradiation Therapy?, E. Yu, A. R. Dar, R. Ash, G. Videtic, P. Truong, L. Stitt, A. Tomiak, M. Vincent, R. Malthaner, I. Craig, E. Brecevic, W. Kocha, R. Inculet, M. Lefcoe

Oncology Presentations

OBJECTIVE: To assess the value of extended volume irradiation with anastomotic coverage in high risk resected esophageal cancer patients.

METHOD: A retrospective study was undertaken at LRCC from 1989-1999 for high risk resected esophageal cancer patients. Adjuvant treatments consisted of 4 cycles of chemotherapy (epirubicin/fluorouracil/cisplatin or cisplatin/fluorouracil), and local regional irradiation with or without coverage of the anastomotic site. Radiation dose ranged from 45-60Gy at 1.8-2.0 Gy/fraction given with initial anterior-posterior/posterior-anterior arrangement with either extended (with anastomotic coverage) or small (without anastomotic coverage) field followed by oblique fields for boost.

RESULT: One hundred eighty-eight charts were reviewed. Seventy-two patients were …

Construction Of A Derivative Of Agrobacterium Tumefaciens C58 That Does Not Mutate To Tetracycline Resistance, Zhao-Qing Luo, Thomas E. Clemente, Stephen K. Farrand

Nebraska Center for Biotechnology: Faculty and Staff Publications

Agrobacterium tumefaciens C58 mutates to tetracycline resistance at high frequency, complicating the use of many broad-host-range cloning and binary vectors that code for resistance to this antibiotic as the selection marker. Such mutations are associated with a resistant gene unit, tet_C58, that is present in the genome of this strain. By deleting the tet_C58 locus, we constructed NTL4, a derivative of C58 that no longer mutates to tetracycline resistance. The deletion had no detectable effect on genetic or physiological traits of NTL4 or on the ability of this strain to transform plants.

Postural Balance And Acceleration Threshold Detection For Anterior Horizontal Translation In Diabetic And Non-Diabetic Elderly, Venkatesh Balasubramanian

Doctoral Dissertations

Slips and falls, and even the fear of failing, can represent a major medical and functional deterrent to living independently, especially among the elderly population. Various groups of elders are at known risk for falling including, but not limited to, those with vestibular dysfunction, those with low visual acuity including visual neuropathies, and those with peripheral neuropathies. The first two groups are fairly well studied, but the relationship between the level of peripheral neuropathy and extent of falling has received relatively less attention.

In this study, using sliding linear investigative platform for analyzing lower limb stability (SLIP-FALLS), the psychophysical thresholds …

Cationic Surfactant Mediated Hybridization And Hydrophobization Of Dna Molecules At The Liquid/Liquid Interface And Their Phase Transfer, Murali Sastry, Ashavani Kumar, Mrunalini Pattarkine, Vidya Ramakrishnan, Krishna N. Ganesh

Faculty Works

Hybridization of complementary oligonucleotides mediated by a cationic surfactant at the water/hexane interface leads to hydrophobic, double-helical DNA which may be readily phase transferred to the organic phase and cast into thin films on solid substrates.

Evaluation Of The Precision Of Magnetic Resonance Phase Velocity Mapping For Blood Flow Measurements, George P. Chatzimavroudis, John N. Oshinski, Robert H. Franch, Peter G. Walker, Ajit P. Yoganathan, Roderic I. Pettigrew

Chemical & Biomedical Engineering Faculty Publications

Evaluating the in vivo accuracy of magnetic resonance phase velocity mapping (PVM) is not straightforward because of the absence of a validated clinical flow quantification technique. The aim of this study was to evaluate PVM by investigating its precision, both in vitro and in vivo, in a 1.5 Tesla scanner. In the former case, steady and pulsatile flow experiments were conducted using an aortic model under a variety of flow conditions (steady: 0.1–5.5 L/min; pulsatile: 10–75 mL/cycle). In the latter case, PVM measurements were taken in the ascending aorta of ten subjects, seven of which had aortic regurgitation. Each velocity …

Focal Osteolysis At The Junctions Of A Modular Stainless-Steel Femoral Intramedullary Nail, Darron M. Jones, Lawrence Marsh, James V. Nepola, Joshua J. Jacobs, Anastasia K. Skipor, Robert M. Urban, Jeremy L. Gilbert, Josheph A. Buckwater

Biomedical and Chemical Engineering - All Scholarship

Background:

During routine follow-up of patients treated with a three-piece stainless-steel modular femoral nail, osteolysis and periosteal reaction around the modular junctions of some of the nails were noted on radiographs. The purpose of this study was to evaluate the prevalence, etiology, and clinical relevance of these radiographic findings.

Methods:

Forty-four femoral fractures or nonunions in forty-two patients were treated with a modular stainless-steel femoral intramedullary nail. Seventeen nails were excluded, leaving twenty-seven intramedullary nails in twenty-seven patients for this study. All patients had had a femoral diaphyseal fracture; nineteen had had an acute fracture and eight, a nonunion. These …

Consensus Evidence Evaluation In Resuscitation Research: Analysis Of Type I And Type Ii Errors, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

Objective: This paper addresses the following statistical question: ‘if genuine improvements in cardiopulmonary resuscitation (CPR) were discovered that doubled the probability of resuscitation success in a series of randomized clinical trials, would they be recognized and incorporated into consensus guidelines?’ Methods: Statistical powers for hypothetical individual clinical trials comparing experimental and control CPR were computed as a function of the study N when the true probabilities for immediate survival, 24 h survival, and discharge survival in the experimental group were twice those in the control group. Next, the binomial distributions describing the numbers of statistically significant studies in a series …

Biomechanics Of Heading A Soccer Ball: Implications For Player Safety, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

To better understand the risk and safety of heading a soccer ball, the author created a set of simple mathematical models based upon Newton’s second law of motion to describe the physics of heading. These models describe the player, the ball, the flight of the ball before impact, the motion of the head and ball during impact, and the effects of all of these upon the intensity and the duration of acceleration of the head. The calculated head accelerations were compared to those during presumably safe daily activities of jumping, dancing, and head nodding and also were related to established …

Tillage And Fallow Effects On Selected Soil Quality Characteristics Of Former Conservation Reserve Program Sites, John E. Gilley, John W. Doran, Bahman Eghball

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Tillage and fallow have been suggested as management options for converting Conservation Reserve Program (CRP) areas to cropland. This study was conducted to measure selected soil quality characteristics of former CRP sites in Mississippi, Nebraska, and South Dakota that were tilled and then left fallow for 21 or 22 months. Soil samples from depth intervals of 0-7.6 cm and 0-30.5 cm were collected for laboratory assessment of the following soil quality indicators: bulk density, EC, pH, total C, organic C, total N, NO₃-N, NH₄-N, PO₄-P, biomass C, biomass N, anaerobic NH₄-N, lab …

Phosphorus Risk Assessment Index Evaluation Using Runoff Measurements, Bahman Eghball, John E. Gilley

Department of Agricultural and Biological Systems Engineering: Faculty Publications

An index to evaluate the phosphorus (P) pollution potential of agricultural fields was proposed by Lemunyon and Gilbert in 1993. Data from three rainfall simulation studies were used to evaluate the relative importance of the variables in the P index. These studies included plots containing sorghum (Sorghum bicolor L.), winter wheat (Triticum aestivum L.), and corn (Zea mays L.) residues on which chemical fertilizer and composted or noncomposted beef cattle feedlot manure were applied under no-till and disked conditions. The factors of erosion, runoff, soil P level P application source, and method, and rate of P addition …

Reducing Long-Term Atrazine Runoff From South Central Nebraska, Walter Scott Gorneau, Thomas G. Franti, Brian L. Benham, Steve D. Comfort

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Heavy reliance on chemical weed control in field crops of South Central Nebraska has resulted in the appearance of atrazine at concentrations greater than established drinking water standards. Our objective was to evaluate the best management practices for atrazine runoff for the tillage and herbicide management practices common to the region under study. Field experiments were performed to measure edge–of–field atrazine and water loss from disk–till, ridge–till, and slot plant (no–till) management systems. Results indicated less water runoff from no–till (34% less) and ridge–till (36% less) than from disk–till. Similarly, atrazine loss was also less: 24% less for no–till and …

Application Of Fuzzy Logic In Automated Cow Status Monitoring, R. M. De Mol, Wayne Woldt

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Sensors that measure yield, temperature, electrical conductivity of milk, and animal activity can be used for automated cow status monitoring. The occurrence of false-positive alerts, generated by a detection model, creates problems in practice. We used fuzzy logic to classify mastitis and estrus alerts; our objective was to reduce the number of false-positive alerts and not to change the level of detected cases of mastitis and estrus. Inputs for the fuzzy logic model were alerts from the detection model and additional information, such as the reproductive status. The output was a classification, true or false, of each alert. Only alerts …

Effects Of Compression Processing Parameters And Antioxidants On Molecular Degradation Of Biodegradable Poly-L-Lactide (Plla), Thomas J. Hannan Jr.

Masters Theses

The purpose of this research was to find a combination of poly (L-lactic acid), also known as poly-L-lactide or (PLLA) and antioxidants that would, together, produce a product whose degradation rate would be advantageous for use in biodegradable medical implants. Intrinsic viscosity tests were conducted on compression molded samples of PLLA that were molded at various processing parameters in order to find optimal parameters. The optimal processing parameters were found to be time 10 minutes, temperature 220°C, and pressure 1000 psi.

The molecular weight of PLLA sample was taken while pressure, time, and temperature were varied. As pressure increased, no …

Novel Processing Of Hydroxyapatite-Zirconia Composites Using Nano-Sized Particles, Mark R. Towler, I. R. Gibson, S. M. Best

Chemical and Biochemical Engineering Faculty Research & Creative Works

A method to produce composites of hydroxyapatite (HA) with ZrO2 (Y-TZP) additions between 0 and 10 wt % at a low sinter temperature of 750 °C is presented. The method limits the amount of reaction between the HA and ZrO2. A ZrO2 loading level that would improve strength while limiting the decomposition of the HA is studied.

Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh

Faculty Works

The hybridization of DNA by sequential electrostatic and hydrogen-bonding immobilization of single-stranded complementary oligonucleotides at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during each stage of complexation in the following sequence: primary single-stranded DNA followed by complementary single-stranded DNA followed by the intercalator, ethidium bromide. Langmuir-Blodgett (LB) films of the ODA-DNA complex were formed on different substrates and characterized using quartz-crystal microgravimetry (QCM), Fourier transform infrared …

Runoff And Soil Loss As Affected By The Application Of Manure, John E. Gilley, L. Mark Risse

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Manure has been used effectively to improve crop production and soil properties because it contains nutrients and organic matter. While it is generally accepted that the improved soil properties associated with manure application lead to changes in runoff and soil erosion, few studies have quantified these impacts. Water quality models used to assess watershed management and estimate total maximum daily load must accurately predict loading rates from fields where manure has been applied. This study was conducted to assemble and summarize information quantifying the effects of manure application on runoff and soil loss resulting from natural precipitation events, and to …

Direct Current Electrical Stimulation Increases The Fusion Rate Of Spinal Fusion Cages, Jeffrey M. Toth, Howard Seim, Jeffrey D. Schwardt, Wendy B. Humphrey, Joel A. Wallskog, A. Simon Turner

Biomedical Engineering Faculty Research and Publications

Study Design. A randomized experimental evaluation of direct current stimulation in a validated animal model with an experimental control group, using blinded radiographic, biomechanical, histologic, and statistical measures.

Objectives. To evaluate the efficacy of the adjunctive use of direct current stimulation on the fusion rate and speed of healing of titanium interbody fusion cages packed with autograft in a sheep lumbar interbody fusion model.

Summary of Background Data. Titanium lumbar interbody spinal fusion cages have been reported to be 90% effective for single-level lumbar interbody fusion. However, fusion rates are reported to be between 70% and 80% in patients with …

Fluid Mechanic Assessment Of The Total Cavopulmonary Connection Using Magnetic Resonance Phase Velocity Mapping And Digital Particle Image Velocimetry, Ann E. Ensley, Agnès Ramuzat, Timothy M. Healy, George P. Chatzimavroudis, Carol Lucas, Shiva Sharma, Roderic Pettigrew, Ajit P. Yoganathan

Chemical & Biomedical Engineering Faculty Publications

The total cavopulmonary connection (TCPC) is currently the most promising modification of the Fontan surgical repair for single ventricle congenital heart disease. The TCPC involves a surgical connection of the superior and inferior vena cavae directly to the left and right pulmonary arteries, bypassing the right heart. In the univentricular system, the ventricle experiences a workload which may be reduced by optimizing the cavae-to-pulmonary anastomosis. The hypothesis of this study was that the energetic efficiency of the connection is a consequence of the fluid dynamics which develop as a function of connection geometry. Magnetic resonance phase velocity mapping (MRPVM) and …

A Statewide Educational Program To Implement Comprehensive Nutrient Management Planning, Richard K. Koelsch, Charles A. Shapiro, R. Wozniak

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

EPA and USDA NRSC have stated that the most effective way for animal feeding operations (AFO's) to minimize water quality and public health risks is to develop and implement comprehensive nutrient management plans. In response a proposed CNMP workbook has been designed to serve as an animal operation's environmental operating plan. This project will initiate field testing of the proposed Nebraska CNMP with livestock producers and advisors. It will also assist with development of supporting resources and training for educational and technical advisors.

Fuzzy Composite Programming To Combine Remote Sensing And Crop Models For Decision Support In Precision Crop Management, David D. Jones, E. M. Barnes

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Precision crop management is by definition a multi-objective decision-making process that must incorporate a diversity of data, opinion, preference and objective. This paper details an approach to decision making that allows users to express individual or corporate values and preferences; highlights the degree of imprecision associated with each input; highlights the degree of imprecision associated with each alternative; facilitates structuring of the decision process; reduces several levels of complex information into a single chart; allows examination of trade-off between alternatives and interests; and forces examination of inter-relationships between interest. The addition of using remote sensing data provides an efficient method …

The Effect Of Anisotrophy On The Potential Distribution In Biological Tissues And Its Impact On Nerve Excitation Simulations, Robert B. Szlavik, Hubert De Bruin

Biomedical Engineering

Presents a finite difference solution of the potential distribution associated with electrical current stimulation in an anisotropic in-homogeneous tissue environment and compare it to the isotropic case. The results demonstrate that there can be significant errors associated with the assumption of isotropic tissue properties in calculating the potential distribution along an axon in nerve excitation simulations. These errors can have a significant impact on predicted nerve fiber recruitment patterns when evaluating the efficacy of specific surface or intramuscular stimulus electrode configurations. The results of this study also suggest when a more comprehensive tissue model should be implemented in an electrode …