Digital Commons Network^™

Innovative Solutions In Medical Diagnostics, Seafood Industry, Environmental, And Forensic Applications Through Integrating Raman Spectroscopy And Machine Learning, Luis Perez Almodovar

Electronic Theses & Dissertations (2024 - present)

There is an urgent need for rapid and on-site analytical methods to address various environmental and forensic challenges. This dissertation explores the application of Raman spectroscopy combined with machine learning for three critical areas: evaluating perfluoroalkyl or polyfluoroalkyl substances (PFAS) exposure in wildlife, authenticating tuna species in the seafood industry, identifying body fluids at crime scenes, and medical diagnostics.

First, Raman spectroscopy and support vector machine discriminant analysis (SVMDA) were employed to detect PFAS levels in the blood plasma of smallmouth bass from two different tributaries, rivers, or streams that flow into larger bodies of water. The method achieved 100% …

Advancements In Forensic Analysis: Development Of Mass Spectrometric And Chemometric Approaches For The Identification Of Synthetic New Psychoactive Substances And Plant Materials, Mónica Ventura

Electronic Theses & Dissertations (2024 - present)

While forensic science is a well-established discipline, a number of federal agencies have highlighted challenges that continue to plague the field and the extent to which these challenges remain unaddressed. Examples are the illegal trade of wildlife timber and the drug epidemic. Characterization of these materials requires nuanced method development for compound determination, matrix material-specific protocols, and heavy use of expensive consumables. The application of a technique such as direct analysis in real time – high-resolution mass spectrometry (DART-HRMS) provides the opportunity to circumvent many of the challenges presented by conventional methods. In general, little to no sample preparation is …

Assessment Of Spatial Distribution Patterns And Infestation Risk Over Time Of Spotted Lanterfly (Lycorma Delicatula) In New York State, Alanna Richman

Electronic Theses & Dissertations (2024 - present)

Spotted Lanternfly (Lycorma delicatula) is an invasive treehopper native to Asia. It was first introduced to Pennsylvania in 2014, and as of 2020 it has expanded to New York state. Their preferred host tree is the invasive tree of heaven (Ailanthus altissima), but they will feed on over 173 different plants worldwide. Preferred host species in New York state include agricultural vegetation such as grape vines and fruit trees. Positive Spotted Lanternfly site data collected by the New York Department of Agriculture and Markets and iMapInvasives were analyzed using ArcGIS Pro to assess spatial distribution patterns. …

A Descriptive Analysis Of Depressive Symptoms And Body Image By Race/Ethnicity And Nativity Status Among Adolescents, Radhika Prasad

Electronic Theses & Dissertations (2024 - present)

Previous research on the immigrant health paradox among children and adolescents show a mental health advantage yet it is unclear if this applies in a similar magnitude to females (Fillion et al., 2018; Zarei et al., 2023). Immigrants may be exposed to stressors related to immigration and acculturation. Some research suggests that females respond more to stress through internalizing symptoms relative to males (Hall-Lande et al., 2007; Adkins et al., 2009). Given these gender differences in stress response, female immigrants may be particularly vulnerable to the psychological consequences of immigration related stressors. The immigrant health paradox has also overlooked subjective …

"Not Trans Enough": A Qualitative Inquiry Of Non-Binary Individuals' Experiences Of Transphobia Within The Lgbtq+ Community, Alyssa Lombardi

Electronic Theses & Dissertations (2024 - present)

Transphobia has been well-documented to have detrimental impacts on the lives of transgender individuals. However, research typically has not focused on the experience of non-binary individuals, and particularly even less so on their experiences of within-group discrimination. This study used Interpretative Phenomenological Analysis to understand both the experience and expression of transphobia toward non-binary people from within the LGBTQ+ community. Twelve interviews were conducted with six non-binary individuals ranging in age from 23 to 33. Each interview was analyzed and coded, resulting in four superordinate themes: The Experience of Enbyphobia, The Impact of Enbyphobia, The Expression of Enbyphobia, and The …

The Study Of Ribosome-Amplified Metabolism, Rambo, Effect In Vitro And In Live Cells, Jianchao Yu

Electronic Theses & Dissertations (2024 - present)

Quinary interactions arise due to the extreme macromolecular crowding inside live cells. Quinary interactions affect intracellular protein stability, activity, and homeostasis. Ribosomes, traditionally known for their role in protein synthesis, have been found to engage in quinary interactions with metabolic enzymes, modulating their activity and thereby revealing a potential regulatory role for ribosomes in cellular metabolism. This phenomenon, termed the Ribosome-Amplified MetaBOlism (RAMBO) effect, was investigated in this dissertation through a series of in vitro and in vivo studies using primarily Nuclear Magnetic Resonance (NMR) spectroscopy.

Firstly, an NMR-based direct enzyme assay compatible with ribosome was developed and validated using …

Stabilizing Bulk Lead-Free Tin Halide Perovskites, William Rice

Electronic Theses & Dissertations (2024 - present)

Tin halide perovskites are a growing candidate in the semiconducting materials field due to their band gap, and ability to be doped (specifically p-type doped). Halide perovskites are sensitive to light and air which causes the degradation of the material from its preferred cubic perovskite lattice. This thesis introduces a way of stabilizing bulk CsSnCl3 through the Goldschmidt tolerance factor. It will also introduce potential new ways to p-type dope halide perovskites. We attempted to p-type dope our halide perovskite by substitutional, interstitial, and vacancy doping to increase the carrier concentration to compete with current perovskites. During our trials of …

Developing Cellular Systems To Elucidate Rna Structural Dynamics Of Cag Expansion Transcripts In Spinocerebellar Ataxias, Victoria Demeo

Electronic Theses & Dissertations (2024 - present)

Spinocerebellar ataxias (SCAs) are a diverse group of over 40 genetically heterogeneous neurodegenerative disorders, many of which are caused by a trinucleotide CAG repeat expansion in the coding region of specific genes. These expansions lead to the production of polyglutamine (polyQ) tracts that interfere with normal protein function, triggering cellular dysfunction and contributing to disease pathogenesis. The most well-known of these SCAs, such as SCA1, SCA2, and SCA3, exhibit progressive neurodegeneration, yet the precise mechanisms through which these mutations cause disease remain poorly understood.

A significant challenge in studying CAG repeat expansion disorders lies in the complexity of the disease …

“A True Tower Of Babel”: Nation, Race, And Gender In The French Foreign Legion, 1831 To 1854, Nicholas Michael Charnley

Electronic Theses & Dissertations (2024 - present)

The French Foreign Legion, part of the July Monarchy’s 1831 expansion of the Army of Africa, sought to create a non-French force entirely deployable outside of the metropole. The creation of the expatriate corps intended to solve two of France’s most pressing problems at the time: an excess of foreign migrants at home, and a dearth of French soldiers abroad. The Paris government hoped to attract recruits from its considerable non-national male population, particularly political exiles and migrant workers fleeing Europe’s 1830 revolutionary wave. Initially intended as a temporary expedient for the homeland’s demographic crisis, the expatriate fighting force grew …

Dishes Of Yesteryear: Memories, Stories, And Representations Of The Past Within Food Media, Diana Willis Bottomley

Electronic Theses & Dissertations (2024 - present)

In 1963, Julia Child revolutionized cooking television. Child’s The French Chef, which ran for ten years on the Boston public television station, WGBH, changed how American’s viewed, produced, and consumed food. And in the sixty years since Child’s debut, food media has grown in scope and popularity, increasingly altering how we communicate identity in and through food and foodways. In this dissertation, I examine the relation between food, media, and memory, with particular attention to: how the past moderates the connection between food and identity; how food media produce a sense of place integral to identity; and how food …

Androgen Regulation Of The Hpa Axis And Stress-Related Behaviors In Mice: The Role Of Crf And Crf Receptor 1, Krystyna Ann Rybka

Electronic Theses & Dissertations (2024 - present)

Mood disorders such as anxiety and depression are nearly twice as prevalent in women when compared to men, with potential contributing factors involving sex-dependent exposure to gonadal hormones and dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis. Androgen actions through androgen receptors (AR) or actions at estrogen receptors such as ERα, following the conversion of androgens to estradiol, have been shown to regulate stress-related behaviors as well as HPA axis responsiveness. Where androgen actions at AR appear to decrease the stress response and subsequent stress-related behaviors, actions at ERα seem to do the opposite and increase HPA axis reactivity. When the HPA …

The Guild House: A Mixed-Methods Case Study Of Game-Utilizing Information/Learning Design For Autonomy-Supportive Instruction In An Asynchronous Online Course, Sturdy Knight

Electronic Theses & Dissertations (2024 - present)

Gamification has emerged over the past ~10-12 years as a popular design approach to problems of motivation in online or blended learning, with a particular emphasis on distinguishing between extrinsic motivation driven by points, grades, class rank, etc, vs. intrinsic motivation driven by personal values and interest in the curriculum. Self-Determination Theory (SDT) forms the theoretical basis of much of this literature, yet the mediating constructs of learner self-regulation and autonomous vs. controlled regulatory styles, which describe the processes by which extrinsic motivators like game rewards or curriculum requirements are integrated with intrinsic goals, are under-addressed. This has led to …

Outcome-Based Patient-Therapist Matching Implementation: A Study Of Clinician Beliefs And Attitudes, Ashleigh Smith

Electronic Theses & Dissertations (2024 - present)

Background. Implementation science aims to identify methods that support the adoption of evidence-based practices (EBPs), including measurement-based care (MBC) and MBC-informed patient-therapist matching. Examining clinician attitudes can elucidate potential barriers to implementing EBPs, and attitudinal differences may exist across groups of therapists. In the context of a patient-therapist matching implementation project, this multi-method study investigated the relationship between clinician characteristics, attitudes toward EBPs/MBC, and implementation climate. Method. A sample of N = 72 therapists completed measures of demographic and clinical characteristics, attitudes toward EBPs, perceptions of implementation climate, and attitudes toward monitoring and feedback. A subset of N …

Public - Private In Vietnamese Universities: Exploring Sectoral Distinctiveness In Program Offerings, Lan Hoang

Electronic Theses & Dissertations (2024 - present)

The public-private issues in higher education have been a longstanding important topic of academic and policy interest, especially in the context of the public - private duality in most higher education systems. With PHE’s impressive growth over the past few decades, now enrolling one third of global HE enrollments, the study of PHE has also been expanded, with several national case studies and world/regional edited books yet being mostly ad hoc with minimal synthesis or comparative analysis (Levy, 2024). The current case study of Vietnam, employing Levy’s double distinctiveness approach in sectoral distinctiveness analysis with the intrasectoral dissection on the …

Sources And Significance Of Symmetric Instability In The Outflow Layer Of Tropical Cyclones, Minghao Zhou

Electronic Theses & Dissertations (2024 - present)

The tropical cyclone (TC) outflow layer is an integral part of the TC transverse circulation: warm and moist air converges in the boundary layer of the TC; rising motion and condensational heating occur in the TC eyewall and rainbands; the TC warm core and divergent anticyclone establish in the mid-upper troposphere, with the mass imported into the TC center exported away through outflow near the tropopause.

A series of state-of-the-art numerical simulations were utilized to investigate different scenarios of TC outflow evolution, from an idealized axisymmetric setup to a semi-idealized asymmetric setup, to a real-case setup of TC–trough interaction. Large …

Investigating The Uncertainties Of Forecasting Ne Cold Season Precipitation In Numerical Weather Prediction Models, Yanna Chen

Electronic Theses & Dissertations (2024 - present)

A major winter storm on March 2, 2018, posed significant challenges in predicting precipitation type (P-type) in the northeast U.S., particularly the Hudson Valley. While National Weather Service (NWS) forecasts predicted a mix of rain and snow, observations showed predominantly snow, with errors in snow depth forecasts of 8-12 inches near Albany.

This study used the Weather Research and Forecasting (WRF) model to investigate how model physics (microphysics, cumulus, boundary layer, and radiation schemes) and domain configuration influenced snow depth predictions. Results revealed significant sensitivities to cumulus and radiation schemes, as well as the vertical resolution in the middle troposphere. …

Enhancing A Computer Model To Evaluate Rainwater Harvesting And Reuse Strategies For Landscape Irrigation Under Current And Future Climate Conditions, Siyu Li

Electronic Theses & Dissertations (2024 - present)

Urbanization and climate change have detrimental impacts on water quantity and quality. Rainwater harvesting/reuse for landscape irrigation with rain barrels/cisterns, which can assist urban hydrological/water quality restoration and provide a beneficial resource for irrigating landscape, holds significant potentials in urban areas under current and future climates. However, the impacts of rainwater harvesting/reuse strategies for landscape irrigation under a changing climate need to be comprehensively evaluated to assist decision-making. In addition, a comprehensive tool is needed to help stakeholders develop efficient strategies to harvest rainwater for landscape irrigation with rain barrels/cisterns.

To address the needs, the simulation of the sub-daily hydrological …

A Study Of Mercury Speciation Analysis Of Blood Using Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry For Human Biomonitoring And Clinical Applications, Emily Jean Pacer

Electronic Theses & Dissertations (2024 - present)

Mercury (Hg) exposure from environmental sources and consumer products presents a significant public health concern. The toxicity of methylmercury (MeHg) differs considerably from inorganic mercury (iHg), such that their separation and quantification in whole blood is helpful in identifying sources and possible pathways of exposure. Speciation methods provide a more detailed picture regarding human exposure to toxic mercury species and their effects on human health. This dissertation focuses on advanced analytical techniques for human biomonitoring and clinical diagnosis of Hg exposure. The primary aim of this research is to address important knowledge gaps and analytical challenges in studies of Hg …

Everyone’S A Critic: Processes Of Legitimation In The Video Essay Genre On Youtube, Kendall Nagi

Electronic Theses & Dissertations (2024 - present)

The rise of video essays as a popular genre on YouTube is a notable development in the media criticism and intellectual work in online spaces. Theorization on criticism as intellectual work and the performance of expertise and credibility is well developed within sociology (Alexander 2004; Jacobs & Townsley 2017) but esearch on YouTube specifically is quite limited within the social sciences. Most existing research on the video essay genre comes from media studies and communications scholarship (McWhirter 2022; Smarandache 2021; Sylvia & Moody 2022). This study seeks to understand how creators working in the video essay genre, and more specifically, …

Equipping Middle School Teachers With Culturally Responsive Pedagogy In Teaching Computer Science Through Professional Learning: A Longitudinal Case Study, Xintong Bausch

Electronic Theses & Dissertations (2024 - present)

Culturally responsive pedagogy (CRP) is an effective way to foster equitable and meaningful computer science (CS) learning experience for historically marginalized students. This dissertation study employed longitudinal data to investigate five middle school teachers’ development of CRP understandings and enactments over many years participating in the CS Pathways Research Practitioner Partnership (RPP) project. Grounded in conceptual change theory (knowledge-in-pieces perspective), the study explored the five teachers’ CRP conceptual change trajectories as indicated by their definitions and enactments of the pedagogy over time. From the epistemological perspective, the findings presented how teachers reflected, refined, and revised their naïve knowledge of CRP …

Transcription And Translation Regulation Of Germ Cell To Maternal Transition, Noor Kotb

Electronic Theses & Dissertations (2024 - present)

In sexually reproducing organisms, the process of gametogenesis produces mature eggs and sperms required for fertilization to occur. The egg's quality is crucial for the healthy development of the next generation, making oogenesis a tightly regulated process with multiple benchmarks. To investigate the intricacies of oogenesis, we utilized the Drosophila oogenesis system as our primary model. This choice was motivated by several factors. Firstly, the process of egg development in Drosophila is conserved across species, making it a valuable proxy for understanding oogenesis in other organisms, including humans. Secondly, Drosophila melanogaster has long been established as a powerhouse in genetic …

Stakeholders’ Participation In Pcaob Audit Standard Setting, Xiaoshuai Yang

Electronic Theses & Dissertations (2024 - present)

Lobbying is an integral part of the Public Company Accounting Oversight Board’s standard-setting process. Although the PCAOB has been drafting auditing standards for over 20 years, there is still much to learn about how stakeholder behavior in submitting comment letters to the PCAOB varies by interest group, comment period (concept release, proposal, and reproposal), the stakeholder’s position on the proposal, arguments used, and emotional strategies. This paper examines PCAOB Docket 034, which proposed audit report reforms, including the auditor’s disclosure of critical audit matters (CAMs). There was strong stakeholder participation and conflicting opinions when this standard was under discussion. To …

Characterization And Measurement Limitations Using Non-Destructive Mueller Matrix Scatterometry (Mmse) And X-Ray Diffraction (Xxrd) Techniques For Gate All Around (Gaa) Transistor Test Structures: Limitations And Superlattice Effects In Advanced Si/Si(1-X)Ge(X) Superlattice Nanowire Test Structures And Measurability Of Simulated Horizontal Gaa Test Structures, Ezra Mel Beltran Pasikatan

Electronic Theses & Dissertations (2024 - present)

Advanced semiconductor devices are moving toward 3D geometries due to scaling demands and performance requirements. Nondestructive metrology necessary for process control of 3D structures must be advanced to facilitate their transition from technology development to high volume manufacturing. Thin film metrology using Mueller Matrix Spectroscopic Ellipsometry (MMSE) and X-ray Diffraction (XRD) film metrology, as well as patterned structure metrology using Optical Critical Dimension (OCD) and X-ray Fluorescence (XRF) techniques have proved capable of measuring the Si/ Si_(1-x)Ge_(x) superlattices and gate-all-around (GAA) transistor test structures. Because these techniques are indirect, their limitations associated with superlattice device structures need …

History, Theology, And Implementation Of A Volunteer Lay Pastor Ministry In The Territory Of The North American Division Of Seventh-Day Adventists, José Cortés Jr.

Doctor of Ministry Projects

Problem

This study examines the strategic necessity and implementation of a Divisionwide Volunteer Lay Pastor Ministry (VLPM) within the North American Division (NAD) of the Seventh-day Adventist Church (SDA Church). The NAD oversees the church’s work in the United States, Canada, Bermuda, Guam, and Micronesia, serving over 1.2 million members across 6,300 congregations. Despite its strong presence, the NAD faces challenges reaching a diverse population of approximately 370 million. With 69 percent of its congregations either plateauing or declining, the church is at a critical point that requires proactive measures to maintain its mission.

Method

Our study begins with an …

Developing And Implementing An Evangelistic Vision: A Study Of Four Different Cohorts In The Birmingham, Alabama Area, Albert Eugene Frazier

Doctor of Ministry Projects

Problem

The foundation of the Seventh-day Adventist church is based on principles of Holy Spirit-inspired vision and execution, established on the example of Jesus who directly implemented spiritual graces in ministry. Within Birmingham First Seventh-day Adventist church, pastoral observation revealed a disconnect in developing and implementing vision and the spiritual graces to achieve evangelistic activity within church leadership and laity. This divide has resulted in a decrease in effective evangelism.

Method

A program focusing on vision development/implementation and teaching/implementation of the spiritual graces was developed utilizing a combination of presentations and practical applications. The project lasted from May 2022 through …

Co-Producing Ethics Guidelines Together With People With Learning Disabilities, Mary Bottomley, Jodie Bradley, Lisa Clark, Bryan Collis, Bojana Daw Srdanovic, Victoria Farnsworth, Annie Ferguson, Dan Goodley, Andrew Fox, Nikita K. Hayden, Charlotte Lawthom, Rebecca Lawthom, Claudia Magwood, Robert Mclean, Ian Middleton, Alison Owen, Matty Prothero, Simon Rice, Simon Richards, Katherine Runswick-Cole, Kelly Scargill, Rohit Shankar, Toni Ann Wood

Peninsula Medical School

Background: We are a research team of clinical, academic and advocacy-based researchers with and without learning disabilities, working on the Humanising Healthcare (for people with learning disabilities) project. The project is dedicated to finding and sharing healthcare practices that enhance the lives of people with learning disabilities. As part of our ethics applications to access National Health Service study sites for fieldwork, we worked together to write guiding principles for co-producing research ethics with researchers with learning disabilities. In this paper, we introduce these Participatory Ethics Good Practice Guidelines and reflect on our collaboration. Methods: We reflect on developing the …

The Cost Of Providing Care By Family And Friends (Informal Care) In The Last Year Of Life: A Population Observational Study, Miriam J. Johnson, David C. Currow, Jade Chynoweth, Helen Weatherly, Gamze Keser, Ann Hutchinson, Annie Jones, Laurie Dunn, Victoria Allgar

Peninsula Medical School

Introduction: Little is known about replacement costs of care provided by informal carers during the last year of life for people dying of cancer and non-cancer diseases. Aim: To estimate informal caregiving costs and explore the relationship with carer and decedent characteristics. Design: National observational study of bereaved carers. Questions included informal end-of-life caregiving into the 2017 Health Survey for England including estimated recalled frequency, duration and intensity of care provision. We estimated replacement costs for a decedent’s last year of life valuing time at the price of a substitutable activity. Spearman rank correlations and multivariable linear regression were used …

Data-Driven Communicative Behaviour Generation: A Survey, Nurziya Oralbayeva, Amir Aly, Anara Sandygulova, Tony Belpaeme

School of Engineering, Computing and Mathematics

The development of data-driven behaviour generating systems has recently become the focus of considerable attention in the fields of human–agent interaction and human–robot interaction. Although rule-based approaches were dominant for years, these proved inflexible and expensive to develop. The difficulty of developing production rules, as well as the need for manual configuration to generate artificial behaviours, places a limit on how complex and diverse rule-based behaviours can be. In contrast, actual human–human interaction data collected using tracking and recording devices makes humanlike multimodal co-speech behaviour generation possible using machine learning and specifically, in recent years, deep learning. This survey provides …

“We’Ve All Come Together”: A Board Game Intervention For Children And Adults With Autism And Other Developmental Disabilities, Gray Atherton, Emily Dawson, Liam Cross

School of Psychology

The authors discuss how board games offer individuals with autism (whooften struggle with social settings and require structured outlets for building relationships) an affordable, naturalistic, collaborative environmentthat is stimulating, engaging, and educational. Board games, they assert,provide opportunities to practice social skills, including cooperation andsocial deduction, essential skills for both neurotypical and atypical people.The authors include recommendations for introducing board games intospecial educational settings. K

Protecting The Mental Health Of People Seeking Sanctuary In The Uk's Evolving Legislative Landscape, Rohit Shankar

Peninsula Medical School

No abstract provided.