Digital Commons Network^™

Tarantula Toxin Transcripts And Toxin Structure Prediction By Alphafold, Christine Vega, Rebekkah Sepulveda, Ying Jia

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Tarantula venom predominantly comprises peptides and small proteins (venom toxins). To explore this further, we generated a combined gland cDNA library from five different tarantula species. Subsequently we randomly sequenced 752 clones, which led to the identification of sixteen distinct toxin groups, including one novel group. Each group consists of a minimum of three identical sequences. A comprehensive analysis of these toxin groups was carried out, and we simulated the 3D structures of certain predicted toxins. These transcripts can be used to the production of venom toxins through recombinant techniques, offering promising applications in various biomedical applications.

Green Synthesis And Characterization Of Senps Using Pediastrum Boryanum Extract And Evaluation Of Their Biological Activities, Ahmed H Al-Wakeel, Samia Elbahnaswy, Amany Abd El‐Moaty, Ayman M. El-Ghamry, Ayman Y. El-Khateeb, Engy Risha, Eman Zahran

Mansoura Veterinary Medical Journal

Background Selenium nanoparticles (SeNPs) were synthesized using a green method with Pediastrum boryanum extract as the reducing and capping agent. Objectives The algal extract and green synthesized SeNPs were characterized for their morphological characters and biological efficacy. Methods Characterization was conducted using UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and zeta potential analysis. Results TEM revealed spherical and tetragonal SeNPs with sizes ranging from 72.16-89.45 nm. FTIR spectroscopy confirmed the presence of functional groups from the algal extract on the SeNPs surfaces, indicating their role as capping agents. Phytochemical analysis showed that the extract had higher …

The Anti-Inflammatory And Antioxidant Effects Of Melissa Officinalis (Lemon Balm)On Paracetamol Induced Hepatotoxicity In Broiler Chickens, Ghada A. Abou Zead, Sara T. Elazab, Azza E. Hassan, Ahmed I. Ateya, Magdy S. Amer

Mansoura Veterinary Medical Journal

background : This study aimed to investigate the hepatoprotective effects of Melissa officinalis (M officinalis) against paracetamol N-acetyl-p-aminophenol (APAP)-induced hepatotoxicity in broiler chickens. METHODS: A total of 48 one-day-old cobb healthy chicks were divided into four groups of (12 chicks). The control group received a basal diet for 21 days, while the APAP group received APAP at 2 g/kg body weight daily from the 15th day onwards and until the termination of the experiment; M officinalis + APAP group was offered a 10 g/kg diet for 2 weeks followed by APAP for one week; and the APAP + M officinalis …

Association Between Lactoferrin Gene Polymorphisms And Mastitis Susceptibility In Baladi Goat, Osama Muhammad, Mohamed Fouda, Basma Hendam, Ahmed Ateya

Mansoura Veterinary Medical Journal

Objective: To explore the association between lactoferrin (LTF) gene polymorphisms and mastitis resistance and susceptibility in Baladi goats. Design: Crossover study Animals: Thirty female Baladi goats. Procedures: Blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. Polymerase chain reaction (PCR) was performed to amplify the 430-bp LTF gene. Results: PCR-DNA sequencing genetic assessment revealed nucleotide sequence variants in the LTF gene associated with mastitis susceptibility among the enrolled Baladi goats (GenBank accession numbers gb|PP037928| and gb|PP037929| for healthy and mastitic does, respectively). One non-synonymous single nucleotide polymorphism (SNP) G299A was discovered …

Detection Of Hepatitis A Virus And Enteroviruses In Raw Milk And Some Dairy Products From Dakahlia Governorate, Egypt Using Real Time Pcr, Ahmed El Hady, Mayada Gwida, Waled Morsy El-Senousy, Seham F. Hasan, Maha Al-Ashmawy

Mansoura Veterinary Medical Journal

Background: The present study aimed to evaluate the presence of hepatitis A virus (HAV) and enterovirus (EV) in raw milk and dairy products and highlight the potential significance of real-time polymerase chain reaction (PCR) in the detection of foodborne viruses.

Methods: A total of 165 samples of dairy products (including milk cream and kareesh cheese, 15 samples each) and 135 raw milk samples were randomly collected from different households with either hand milking or bulk tanks in Dakahlia Governorate, Egypt, over a period of 28 months from July 2020 to November 2022), using real-time PCR as a diagnostic method.

Results: …

Individual Genomic Loci In The Caprine Β-Lactoglobulin Gene Linked To Mastitis Incidence In Baladi Goats, Yosra Abdel-Maksoud, Mohamed Fouda, Adel Abdelkhalek, Ahmed Ateya

Mansoura Veterinary Medical Journal

This research attempts to inquire at the genetic differences in the β-lactoglobulin gene (β-LG) along with how it affects mastitis tolerance/susceptibility in Baladi goats. For the purpose of extracting DNA, blood samples have been gathered from thirty female Baladi goats and loaded into tubes containing the anticoagulant disodium EDTA. PCR was implemented to amplify the β-LG gene's 427 base pairs. PCR-DNA sequencing analysis spotted β-LG gene differences taking the form of C390A single nucleotide polymorphism (SNP) correlated with mastitis tolerance in Baladi goats (GenBank with accession numbers submissions gb|PP265530|, and gb|PP265531|| for healthy and mastitic does accordingly). The β-LG gene …

Poor Water Quality As A Trigger Of Harmful Algal Blooms In Lake Manzala, Mohammed A. Hussein, Alaa Eldin Eissa, Reham M. Eltarabily, Mohamed F. Abdelghany, Mahmoud A. Elnakeeb, Eman M. Ismail, Reham H. Ragab, Amina A. Dessouki

Mansoura Veterinary Medical Journal

Objective: The current study aimed to assess the direct effects of poor water quality on the eruption of heavy harmful algal blooms and to assess the physicochemical properties of water in Lake Manzala, as well as the molecular identification of the retrieved green algae.

Procedures: Subsurface water samples were collected from three different sites in September 2023 for water quality assessment. Moreover, morphological and molecular identification of suspected harmful algae is the most reliable diagnostic tool for this eruption.

Results: This study was conducted in the northern and northeastern regions of Lake Manzala. These regions receive large amounts of municipal, …

Influence Of Spirulina Platensis Inclusion Upon Some Biochemical Markers Besides Quality Of Meat In Broiler Chickens, Enas Hassan, Gehad El-Sayed, Azza Hassan, Shimaa El-Sayed

Mansoura Veterinary Medical Journal

OBJECTIVES The current study aimed to evaluate the influence of Spirulina Platensis inclusion on biochemical indices besides meat quality in broilers.

METHODS One hundred newly hatched meat-yielding chicks purchased from a local hatchery in Gamasa City were randomly divided into five groups, with 20 birds per group.

RESULTS Spirulina platensis acted as an excellent growth-promoting agent. Spirulina platensis inclusion at doses of 0.5, 1, and 2 g in the poultry diet enhanced growth performance indices, as body weight, body weight gain, feed consumption. Spirulina positive influence on meat quality was evident through decreased thawing and cooking losses, an increase in …

Antioxidant, Anti-Apoptotic, And Anti-Inflammatory Potential Of Nigella Sativa Extract Orchestrates Its Role Against Fipronil-Induced Toxicity In Rats’ Brain, Naglaa Mostafa Maher, Ola A. Habotta, Fathy Sleem

Mansoura Veterinary Medical Journal

Background: Fipronil (FPN) a phenyl pyrazole insecticide, is widely applied in agriculture and veterinary products. However, its use is accompanied by neurotoxic effects. Hence, the current study was designed to assess the possible neuroprotective action of Nigella sativa extract (NS) against FPN-induced neurobehavioral toxicity in Wister male albino rats.

Methods: Thirty male albino rat weighing one hundred fifty to one hundred eighty grams. Animals were given FPN (12.6 milligrams per Bodyweight) orally or pre-treated using NS (100 and 200 mg/kg BW, respectively). The research study had been carried out within a four-week period.

Results: The findings demonstrated considerable …

Genetic Characterization Of Growth Hormone Gene In Dromedary Camels, Ahmed Kamel, Ahmed Ateya, Adel Abdelkhalek, Huda El- Emam

Mansoura Veterinary Medical Journal

Background: This work aimed to explore the genetic characteristics of the GH gene in dromedary camels.

Methods: Thirty dromedary she-camels were used to provide blood samples using disodium EDTA as an anticoagulant for DNA extraction. Using the polymerase chain reaction (PCR), the 613-bp GH gene was amplified. The dromedary she-camels that were enrolled were examined for polymorphisms in the GH gene (613 bp) using the PCR-DNA sequencing method.

Results: The GH gene sequence comparison among the camels under investigation revealed nucleotide sequence variation in the form of the A430C single nucleotide polymorphism (SNP), which has been submitted to GenBank under …

Characterization And Antimicrobial Susceptibility Of Salmonella Enterica Isolated From Broiler Chicks, Rana Hagras, Noura F. Mostafa, Asmaa Sadat, Amal Awad

Mansoura Veterinary Medical Journal

Objective: Salmonella is frequently implicated in several harmful processes that affect both humans and animals, including poultry. This study aimed to isolate and identify Salmonella serovars from clinically diseased broiler chicks collected from poultry farms as well as evaluate the susceptibility of the isolates to the antimicrobial agents frequently used in poultry farms. Design: Two- hundred broiler chicks’ samples were collected from broiler farms located in Dakahlia province and subjected to bacteriological examination for the isolation of salmonellae. Salmonella isolates were then subjected to serological identification to detect Salmonella serovars circulating in broiler farms. In addition, the isolated strains were …

Cryptosporidium Infection In Ducks And Pigeons Collected From Live Bird Markets In Two Nile Delta Governorates (Dakahlia And Gharbia), Egypt, Ahmed Essam, Ibrahim Abbas, Bassem Elmishmishy, Salah Abu Elwafa

Mansoura Veterinary Medical Journal

Background: Cryptosporidiosis is a zoonotic parasitic disease that infects a wide range of hosts, including birds. Cryptosporidium infections vary across avian species and cause considerable economic losses. Data on cryptosporidiosis in ducks and pigeons are scarce.

Objectives: The present study aimed to investigate Cryptosporidium infections in household ducks and pigeons collected from live bird markets in two Nile Delta governorates (Dakahlia and Gharbia) in Egypt.

Methods: The intestinal contents of household ducks (n=109) and pigeons (n=57) collected from live bird markets in two Nile Delta governorates (Dakahlia and Gharbia) were tested using a sugar flotation test coupled with modified …

Impact Of Ellagic Acid Loaded Zinc Nanoparticles On Cardiac And Hepatic Tissue On Type 1 Diabetic Rats, Gehad R. M. Elsayed, Afaf D. Abdelmagid, Hatem B. Huseiny, Ahmed Fotoh, Faten E. Shoker

Mansoura Veterinary Medical Journal

Background:

Biochemical, molecular, and histopathological alterations are the most noticeable clinical and pathological characteristics attributed to diabetes mellitus. An imbalance in these parameters increases the risk of developing complications. Therefore, the main objective of this study was to determine the cardiac and hepatic protective effects of ellagic acid-loaded zinc nanoparticles (EA-ZnNPs) on diabetes, which were evaluated biochemically, molecularly, and histopathologically.

Methods: Four groups of 40 adult male rats were established in this study. The groups were subsequently classified as control, streptozotocin (STZ) diabetic, STZ treated with ellagic acid-loaded zinc nanoparticles (EA-ZnNPs), and STZ treated with insulin. After six weeks of …

Enhancing Yogurt Quality With Inulin: A Study On Chemical, Microbial, And Sensory Improvements, Md Sodor Uddin, Snigdha Sharmin, Fahmida Jahan Fahim, Sohel Rana, Kazi Zinnah, Ferdaus Mohd Altaf Hossain

Mansoura Veterinary Medical Journal

Objective: This research investigates the impact of inulin, a fructo-oligosaccharide (FOS), on enhancing the chemical, microbial, and sensory attributes of yogurt, aiming at improving its overall quality and shelf-life. Design: Through a controlled experimental setup, yogurt samples were prepared with 1.5% concentrations of inulin and analyzed against control samples over different storage conditions. Results: Our findings indicate that the control group has a better sensory profile than the inulin-treated samples, and microbial stability by fostering the growth of beneficial Lactic Acid Bacteria (LAB), thereby enriching the yogurt's probiotic profile. Additionally, sensory evaluations reveal that inulin addition maintains desirable yogurt characteristics, …

Re-Globalizing Yorkville: The Languages On Shop Signs In A Non-Migrant Neighborhood, Lisabeth G. Dashman

Electronic Theses & Dissertations (2024 - present)

The term “linguistic landscape” is used to describe the usage and placement of language in public places of a specific geographic area, such as on buildings, street names, pedestrian notices, vehicular warnings, and advertising billboards. Studies of a space’s linguistic landscape typically involve an accounting and analysis of the visible signs and/or the presentation of bystanders’ or researchers’ thoughts and observations. This study instead probes the intentions of the sign creators: the owners of the façade sign and the business it advertises. The façade sign, often called the shop sign, is frequently the first image for a business that a …

Let Your Head Hang Down: A Narrative Examination Of Cultic & Conspiratorial Romance, Kyle Macy

Electronic Theses & Dissertations (2024 - present)

A response to recent cultural trends of radicalization, extremism, and violence in American society, this dissertation, a novel rendered in ephemeral fragments of oral histories, interrogates the romanticist postures that compel a community of musical artists, the so-called “Folk Revival Revival,” toward infamy and tragedy. Where more traditional sociological approaches to cultic formations stress the importance of centralized charismatic authority, and more traditional psychological approaches rely upon a conspiratorial Cold War ethos of cognitive bias and coercive control (i.e. “brainwashing”), this project meets such assumptions with incredulity, asserting instead that cultic and conspiratorial entrancement awakens first from within, and may …

A 3d Co-Culture Model Of Senescent Cell Burden And Salivary Gland Fibrosis, Stephen Rose

Electronic Theses & Dissertations (2024 - present)

Patients with head and neck cancer often suffer collateral radiation damage to the salivary glands, leading to fibrosis. Histological examinations indicate that radiation encourages fibrosis by promoting cellular changes leading to both senescent and myofibroblast phenotypes, coupled with a significant loss of regenerative and acinar cells. Chronic hypofunction remains a primary concern, even with modified radiation approaches to sparing salivary glands. Current treatments primarily offer symptomatic relief without rectifying irreversible glandular tissue damage. This limitation arises, in part, from a deficiency in predictive preclinical models to study this multifaceted problem.

We introduce a 3D in vitro microtissue model to address …

Assessment Of Spatial Distribution Patterns And Infestation Risk Over Time Of Spotted Lanterfly (Lycorma Delicatula) In New York State, Alanna Richman

Electronic Theses & Dissertations (2024 - present)

Spotted Lanternfly (Lycorma delicatula) is an invasive treehopper native to Asia. It was first introduced to Pennsylvania in 2014, and as of 2020 it has expanded to New York state. Their preferred host tree is the invasive tree of heaven (Ailanthus altissima), but they will feed on over 173 different plants worldwide. Preferred host species in New York state include agricultural vegetation such as grape vines and fruit trees. Positive Spotted Lanternfly site data collected by the New York Department of Agriculture and Markets and iMapInvasives were analyzed using ArcGIS Pro to assess spatial distribution patterns. …

The Secret Feminine: The Distortion Of The Womb In The Medieval Period, Lindsey Craig

Research Papers

No abstract provided.

The Effects Of Masks On Speech Perception: A Case Study Of A 5-Year-Old, Megan Jameson

Online Theses and Dissertations

Through a case study design, this research examined whether mask usage in communication partners influenced speech perception in a 5-year-old child, and if so, what that effect was. Participants were recruited using a mix of electronic and physical flyers. Participants had to meet all inclusion criteria, including being English speaking, typical developing, and the age of 5 years for the duration of the study. Typical development was determined through the usage of three screeners; hearing, articulation, and language. Three children were screened, but only one met all inclusion criteria for the study. This individual went on to participate in phase …

Green Poems, Lillian I. Emerick Valentine

Graduate Student Theses, Dissertations, & Professional Papers

With broad lyric range, the ecopoems in Green center around the ideology and ethics of the American West. The speaker’s position within that as a descendent of settler laborers is interrogated, as well as language itself. Grammar is used as a tool to perform deconstructive work, examining how labor intersects with colonialism and climate change. Melding intellectual analyses of etymology with the physical act of agricultural labor, these poems range from the conversational and playful to lyric explorations of loss.

Interwoven with this is the speaker’s self-examination of femininity and matrilinear inheritance. How do we use the language we’ve been …

Old Stock, Grace K. Butler

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

Motion And Rest, Claire M. Tuna

Graduate Student Theses, Dissertations, & Professional Papers

Motion and Rest is a collection of lyric, experimental, and hybrid poems that feature transit as a recurring trope. The speaker of these poems travels through town on a bicycle, through the sky on a plane flying backwards in time, on a train through the countryside, and, among water aerobicizers, from one end of the pool to the other. Along with physical space, the speaker traverses emotional space, moving through boredom, worry, curiosity, aching, inertia, presence, and play, slowing at times, but avoiding a full stop.

Conveyance is also investigated as it pertains to communications sent and received. The speaker …

Field Of Play, Lauren Ariana Tess

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

Sudden Oak Death, Jeffrey William Guay

Graduate Student Theses, Dissertations, & Professional Papers

A novel set contemporaneously in rural Montana, Sudden Oak Death follows two protagonists, Wade and his teenage daughter Paige. Each are fighting different addictions, as Wade is in alcoholism recovery, and Paige recently came home from a drug treatment program. In order to succeed, Paige must reintegrate herself into public high school, despite suffering from undiagnosed dyslexia. Wade is raising his family as a single father, and struggles to maintain his emotional stability in the face of his own recovery.

Tumor Integrin Targeted Theranostic Iron Oxide Nanoparticles For Delivery Of Caffeic Acid Phenethyl Ester: Preparation, Characterization, And Anti-Myeloma Activities., Barkley Smith, Yuancheng Li, Travis Fields, Michael Tucker, Anna Staskiewicz, Erica Wong, Handong Ma, Hui Mao, Xinyu Wang

PCOM Scholarly Works

Multiple myeloma (MM) is characterized by the accumulation of malignant plasma cells preferentially in the bone marrow. Currently, emerging chemotherapy drugs with improved biosafety profiles, such as immunomodulatory agents and protease inhibitors, have been used in clinics to treat MM in both initial therapy or maintenance therapy post autologous hematopoietic stem cell transplantation (ASCT). We previously discovered that caffeic acid phenethyl ester (CAPE), a water-insoluble natural compound, inhibited the growth of MM cells by inducing oxidative stress. As part of our continuous effort to pursue a less toxic yet more effective therapeutic approach for MM, the objective of this study …

Empowering Consumer Rights And Community Welfare: Enhancing Corporate Accountability Through A Geographical Indication Licensing Policy, Akriti ., Sarang Sureshrao Khadkekar, Pinaki Ghosh

International Journal on Consumer Law and Practice

Geographical Indications are a unique form of intellectual property that can potentially improve social-economic welfare. The Indian GI Act is based on the concept of consumer welfare, which prevents market activities that can mislead or confuse consumers. Such confusion can be about the source of origin, the quality, or the reputation of the goods. However, several studies have shown the rampant use of GI names by unauthorized persons and Corporations, creating dangers of diluting the reputation of GI names. Such unauthorized use of GI names results in an unfair commercial advantage to these infringers. Not only that, a genuine consumer …

James Casada Papers - Accession 903, James Allen Casada

Manuscript Collection

The James Allan Casada Papers consists of materials collected by Dr. James Allan Casada (b. 1942) who was a professor of history at Winthrop University from 1971-1996. Dr. Casada was also the varsity soccer coach from 1975-1987 and was inducted into Winthrop Athletic Hall of Fame. The collection consists mostly of Dr. Casada’s professional research and articles, but also includes materials related to his graduate work, Winthrop teaching records, book reviews, publication materials, and newspaper clippings. A majority of Dr. Casada’s research is related to 19^th century and early 20^th century explorers, mainly of Africa by British Explorers.

Synthetic Dataset Generation Using Photo-Realistic Simulation With Varied Time And Weather Axes, Thomas Lee, Susan Mckeever, Jane Courtney

Articles

To facilitate the integration of autonomous unmanned air vehicles (UAVs) in day-to-day life, it is imperative that safe navigation can be demonstrated in all relevant scenarios. For UAVs using a navigational protocol driven by artificial neural networks, training and testing data from multiple environmental contexts are needed to ensure that bias is minimised. The reduction in predictive capacity when faced with unfamiliar data is a common weak point in trained networks, which worsens the further the input data deviates from the training data. However, training for multiple environmental variables dramatically increases the man-hours required for data collection and validation. In …

Green Synthesis Of Bimetallic Cuo@Nio Nanocomposite For The Removal Of Glyphosate, Kornkanok Boonserm, Anan Sutcha, Rarm Phinjaroenphan

Journal of Metals, Materials and Minerals

Green synthesis of photocatalyst bimetallic CuO@NiO nanocomposite for eliminating organic hazardous glyphosate (Gly) solution has been introduced. The nanocomposite has been successfully developed from mango (Mangifera indica L.) peel extracted solution by a simultaneous reduction process. HRTEM, XRD, and EDX have also been used to explore the nanostructure, crystal conformation, and chemical compositions of CuO@NiO. Using UV-vis spectrometer, we have observed the photo-catalytic activity and kinetic removal rate constant of CuO@NiO in terms of glyphosate elimination under UV light illumination. Compared with pure CuO and NiO nanoparticles, CuO@NiO displayed improved and enhanced photocatalytic activity. This work demonstrates an …