Digital Commons Network^™

Development Of Antioxidant Film Based On Gelatin And Carboxymethylcellulose Incorporated With Tecoma Stans (L.) Juss. Ex Kunth Petals Extract For Biodegradable Food Packaging, Pacharawan Ratanasongtham, Passara Sukplee, Yutthana Wongnongwa

Journal of Metals, Materials and Minerals

The aim of this research was to develop a novel combination of antioxidant blended film for use as biodegradable packaging in the food industry.  The antioxidant film was prepared based on gelatin (G) and carboxymethyl cellulose (CMC) incorporated with Tecoma stans (L.) Juss. ex Kunth petals extract (TKE) at various concentrations (0.5, 1.0, and 2.0 mg∙L‒1) via solution casting method. The structural and surface morphology of G/CMC-TKE film were characterized using FTIR spectrometry technique and scanning electron microscopy (SEM), including determining antioxidant activity, water solubility, water vapor permeability, and biodegradability. According to FTIR analysis, the significant interaction between the gelatin …

Recent Advances In Synthesis And Bio-Applications Of Natural Stabilizers For Metal Nanoparticles, Abhishek Sharma, Navneet Kaur, Ayashkanta Nanda, Manvinder Kaur, Rajeev Sharma, Harvinder Singh Sohal

Journal of Metals, Materials and Minerals

Due to their exceptional physicochemical properties, the synthesis and application of metal nano-particles gained significant traction and a grip in industries and scientific fields or regions. However, the thermodynamic instability of metal nanoparticles poses or leads to challenges in their controlled synthesis and stabilization. To address this stability and the immobilization strategies, natural polymers such as cellulose, starch, alginate, chitosan, and hyaluronic acid have been explored for their non-toxic, biodegradable, and environmentally friendly characteristics. Recent advances in nanotechnology have led to an increased focus on these natural polymer’s utilization as effective stabilizers for diverse metal nanoparticles. This review comprehensively examines …

Sbr Elastomer Response To Renewable Diesel Blends: An Experimental Investigation, Andrias Rahman Wimada, Ni Putu Dian Nitamiwati, Fatimah Tresna Pratiwi, Maharanni Dewi Solikhah, Bina Restituta Barus, Eki Dwi Wijanarko, Riesta Anggarani, Lies Asiyah, Setyo Widodo, Arfi Thahar, Soni Solistia Wirawan

Journal of Metals, Materials and Minerals

To investigate the impact of Styrene Butadiene Rubber (SBR) exposure to renewable fuels during handling and storage processes, compatibility tests were conducted on three different renewable fuel mixtures: 30% biodiesel, 40% biodiesel, and 30% biodiesel + 10% hydrogenated vegetable oil. These tests involved static immersion experiments conducted at room temperature for a duration of 1000 h, following the ASTM D471 standards. Before and after the immersion tests, the physical properties and visually assessed elastomers concentrations were investigated by varying the SBR content. Additionally, the post-immersion fuel properties were examined. The results showed that mass and volume swelling increased with the …

Bifunctional Cationic Modification Of Modal Fabric For Sustainable In Textile Dyeing With Spent Coffee Grounds, Monthon Nakpathom, Rattanaphol Mongkholrattanasit, Sarute Ummartyotin, Pisutsaran Chitichotpanya, Nattaya Vuthiganond

Journal of Metals, Materials and Minerals

In order to dye cellulose or regenerated cellulose with natural dyes, toxic metal mordants are generally employed. In response to this issue, the novel bifunctional cationic agent was pretreated to modify modal (regenerated cellulose) fabric prior to dyeing to enhance the fiber-dye affinity. The cationization reaction was carried out using a compound containing bicationic sites, N,N'-(oxybis (ethane-2,1-diyl))bis(3-chloro-2-hydroxy-N,N-dimethylpropan-1-aminium. This unique cationic agent is capable of producing nonvolatile amine and has no odor problem. The successful cationic modification was confirmed through FTIR spectroscopy and zeta potential measurement. The cationized process was optimized at a cationic and sodium hydroxide concentration …

Designing Biphasic Nanocellulose Hydrogels To Mimic The Complex Cartilage-Bone Interface, Sairash Sairash, Sirinee Jirajessada, Supansa Yodmuang

Journal of Metals, Materials and Minerals

Osteochondral lesions, which affect both the cartilage and the bone, present significant challenges in treatment due to the complex mechanical and biochemical properties of these tissues. A crucial consideration in developing tissue replacements for these lesions is the simultaneous regeneration of cartilage and calcified cartilage, which forms the transition zone to bone. Our current study aims to fabricate a bilayer polymeric hydrogel designed not only to support cartilage regeneration but also to serve as an interface between cartilage and bone. The bilayer hydrogel was created by combining oxidized bacterial nanocellulose, gelatin, and alginate in one layer, while the other layer …

Deep Learning Model Compression For Resource-Constrained Environments., Stephen Burke

ICT

This study examines the effects of three Deep Neural Network compression techniques—Quantisation, Pruning, and Weight Sharing/Clustering—on CNN and ANN models trained for image classification tasks. The models were tested on the CIFAR-10 dataset for multiclass classification and a binary classification task using a dataset derived from COCO. The best validation accuracy achieved was 74.7% with a CNN on CIFAR-10 and 53% with the best ANN. On the COCO dataset, a modified CIFAR-10 CNN model achieved 75%. The models were compressed using the three techniques and benchmarked on a ThinkPad laptop and Raspberry Pi 3B+ based on metrics relevant for resource-constrained …

Editing The Tri5 Gene Of Fusarium Graminearum Using Crispr/Cas9, Madelyn Rae Waters

Honors Program Theses

No abstract provided.

Storage Facilities And Strategies In The Nile Valley Between The Borderscape Of The First And Third Cataracts (4000 Bc-1000 Bc), Ana Oliveira

Theses and Dissertations

Between the 4th millennium and 1st millennium BC, different communities inhabited the region located within the First to the Third Cataracts. These groups obtained, stored, and distributed foodstuff and commodities in various ways. This paper presents archaeological data from this area and period to shed light into storage strategies and facilities in use during this period on the upper course of the Nile Valley in an attempt to better understand subsistence strategies and economies.

21st Century Churches And Federal Tax Law, Lloyd Hitoshi Mayer, Ellen P. Aprill

Journal Articles

Federal tax treatment matters to churches, the term the IRS uses for all types of religious congregations, including synagogues, mosques, and temples. The federal tax provisions most significant for churches and certain entities closely related to them, however, are not those that the public and commentators often assume. Exemption from income tax and the ability of donors to deduct contributions, the benefits that receive the most public attention, in fact provide surprisingly little benefit either to churches in the aggregate or to most individual churches. Their status as organizations taxexempt under section 501(c)(3) of the Internal Revenue Code, moreover, imposes …

Churching Nimbys: Creating Affordable Housing On Church Property, Patrick E. Reidy, C.S.C.

Journal Articles

In recent years, faith communities across the United States have begun to create affordable housing on church property, inspired by sincerely held religious beliefs. Some are building microhomes behind their houses of worship. Others are converting residences once used by religious ministers—from rectories to abbeys to convents—into units for seniors and low-income families. Still others are repurposing their vacant schools, church parking lots, and undeveloped parcels of land for denser multifamily structures, from townhouses to apartment buildings. Within housing-advocacy circles and among faith communities, these continent-wide efforts to create affordable housing on church property have manifested an affirmative declaration: “Yes, …

Sacred Easements, Patrick E. Reidy, C.S.C.

Journal Articles

In the last forty years, Native American faith communities have struggled to protect their sacred sites using religious liberty law. When confronting threats to sacred lands, Native Americans stridently assert constitutional and statutory free exercise protections against public authorities. But unlike litigation involving non-Indian religious property, cases involving sacred sites seek to protect land that tribal faith communities do not themselves own. Because they lack an explicit ownership interest, Native Americans struggle to protect their sacred sites from desecration and destruction. Courts asked to weigh Indian religious liberty claims against non-Indian property claims always side with the landowner. Since sacred …

A Scoping Review On Mobile Health Technology For Assessment And Intervention Of Upper Limb Motor Function In Children With Motor Impairments, Md Raihan Mia, Sheikh Iqbal Ahamed, Alissa V. Fial, Samuel Nemanich

Computer Science Faculty Research and Publications

Upper limb (UL) motor dysfunctions impact residual movement in hands/shoulders and limit participation in play, sports, and leisure activities. Clinical and laboratory assessments of UL movement can be time-intensive, subjective, and/or require specialized equipment and may not optimally capture a child's motor abilities. The restrictions to in-person research experienced during the COVID-19 pandemic have inspired investigators to design inclusive at-home studies with child participants and their families. Relying on the ubiquity of mobile devices, mobile health (mHealth) applications offer solutions for various clinical and research problems. This scoping review article aimed to aggregate and synthesize existing research that used health …

Bet Inhibition Reforms The Immune Microenvironment And Alleviates T Cell Dysfunction In Chronic Lymphocytic Leukemia, Audrey L. Smith, Sydney A. Skupa, Alexandria P. Eiken, Timothy E. Reznicek, Elizabeth Schmitz, Nolan Williams, Dalia Y. Moore, Christopher R. D'Angelo, Avyakta Kallam, Matthew A. Lunning, Gregory Bociek, Julie M. Vose, Eslam Mohamed, Anna R. Mahr, Paul W. Denton, Ben Powell, Gideon Bollag, M. Jordan Rowley, Dalia El-Gamal

Journal Articles: Oncology and Hematology

Redundant tumor microenvironment (TME) immunosuppressive mechanisms and epigenetic maintenance of terminal T cell exhaustion greatly hinder functional antitumor immune responses in chronic lymphocytic leukemia (CLL). Bromodomain and extraterminal (BET) proteins regulate key pathways contributing to CLL pathogenesis and TME interactions, including T cell function and differentiation. Herein, we report that blocking BET protein function alleviates immunosuppressive networks in the CLL TME and repairs inherent CLL T cell defects. The pan-BET inhibitor OPN-51107 reduced exhaustion-associated cell signatures resulting in improved T cell proliferation and effector function in the Eμ-TCL1 splenic TME. Following BET inhibition (BET-i), TME T cells coexpressed significantly fewer …

Chemoreflex Sensitization Occurs In Both Male And Female Rats During Recovery From Acute Lung Injury, Kajal Kamra, Irving H. Zucker, Harold D. Schultz, Han-Jun Wang

Journal Articles: Cellular & Integrative Physiology

Introduction: Sex-specific patterns in respiratory conditions, such as asthma, COPD, cystic fibrosis, obstructive sleep apnea, and idiopathic pulmonary fibrosis, have been previously documented. Animal models of acute lung injury (ALI) have offered insights into sex differences, with male mice exhibiting distinct lung edema and vascular leakage compared to female mice. Our lab has provided evidence that the chemoreflex is sensitized in male rats during the recovery from bleomycin-induced ALI, but whether sex-based chemoreflex changes occur post-ALI is not known. To bridge this gap, the current study employed the bleomycin-induced ALI animal model to investigate sex-based differences in chemoreflex activation during …

Targeted Insertion Of Conditional Expression Cassettes Into The Mouse Genome Using The Modified I-Pitt, Hiromi Miura, Ayaka Nakamura, Aki Kurosaki, Ai Kotani, Masaru Motojima, Keiko Tanaka, Shigeru Kakuta, Sanae Ogiwara, Yuhsuke Ohmi, Hirotaka Komaba, Samantha L. P. Schilit, Cynthia C. Morton, Channabasavaiah B. Gurumurthy, Masato Ohtsuka

Journal Articles: Genetics, Cell Biology & Anatomy

BACKGROUND: Transgenic (Tg) mice are widely used in biomedical research, and they are typically generated by injecting transgenic DNA cassettes into pronuclei of one-cell stage zygotes. Such animals often show unreliable expression of the transgenic DNA, one of the major reasons for which is random insertion of the transgenes. We previously developed a method called "pronuclear injection-based targeted transgenesis" (PITT), in which DNA constructs are directed to insert at pre-designated genomic loci. PITT was achieved by pre-installing so called landing pad sequences (such as heterotypic LoxP sites or attP sites) to create seed mice and then injecting Cre recombinase or …

Thrive: Success Strategies For The Modern-Day Faculty Member, Elizabeth L. Beam, Betsy J. Becker, Jeana Benton, Megan Berger, Garima Bhandari, Eric Bloomquist, Brian P. Boerner, Kathleen Brandert, Emily Brandt, Liliana Bronner, Russell J. Buzalko, Kim Castellano, Creative Coaching, Teresa Cochran, Giovanni Consolino, Beth Culross, Tanya M. Custer, Precious Davis, Dele Davies, Holly Dingman, Jayme Dowdall, Steven Ebers, Mary Edgerton, April Elker, Bryant England, Sandra Friimpong, Kellie Gossman, Julie Gregg, Dianna Hemsath, Karen Honeycutt, Michelle Howell, Grace C. Johnson, Heidi Keeler, Victoria Kennel, Birgit Khandalavala, Jess King, Jessica Koran-Scholl, Gail Kotulak, Nora Kovar, Michael Kozak, Darby Kurtz, Crystal Krause, Trek Langenhan, Jennifer L. Larsen, Kristan Lester, Bethany Lowndes, Linda M. Love, Riley D. Machal, Alberto Marcelin, Jasmine R. Marcelin, Jessica Maxwell, Sarah B. Mcbrien, Analisa Mcmillan, Carrie Meyer, Kyle Meyer, Jane L. Meza, Kim Michael, Luann Mill, Peggy Moore, Judy Neville, Dana Raml, Priscila Rodrigues Armijo, Cory Rohlfsen, Khalid Sahak, Lepaine Sharp-Mchenry, Kari Simonsen, Nicole M. Sleddens, Zachary P. Slocum, Christopher J. Smith, Michael Smith, Matt Sorensen, G. Talmon, Erin J. Torell, Kathleen G. Volkman, Jana L. Wardian, Steven P. Wengel, Debra Wekesa, Tessa M. Wells, Maggie Winnicki, Rowen K. Zetterman, Andrea Zimmer, Kenneth A. Zoucha

Books: Faculty Development

The THRIVE collection is intended to help faculty thrive in their roles as educators, scholars, researchers, and clinicians. Each section contains a variety of thought-provoking topics that are designed to be easily digested, guide personal reflection, and put into action. Please use the THRIVE collection to help:

• Individuals study topics on their own, whenever and wherever they want
• Peer-mentoring or other learning communities study topics in small groups
• Leaders and planners strategically insert faculty development into existing meetings
• Faculty identify campus experts for additional learning, grand rounds, etc.

If you have questions or want additional information on a topic, simply …

Declaring And Terminating Public Health Emergencies: Performative Utterances That Can Change The World, Christine N. Coughlin, Ana S. Iltis

Maryland Law Review

No abstract provided.

Editorial: Intersection Of Diet, Intestinal Microbiota, And Their Metabolites On Cancer Prevention, Nancy D. Turner, Tiffany L. Weir, Jacques Izard

Journal Articles: Internal Medicine

No abstract provided.

The Ambivalent Logics Of Business Representation In International Organizations, Melissa (M.J.) Durkee

Scholarship@WashULaw

The United Nations and its bodies have 'opened up' to a broad range of non-state actors over the last three decades, including for-profit actors and their representatives. The shift is reflected in the UN's sustainable development goals and the Global Compact, emphasizing public-private partnerships; in greater participation of corporations at treaty conferences; in trade group roles as observers at organizations; and in multi-stakeholder projects. Yet international organizations have generally not developed robust responses to legitimacy concerns about businesses becoming closely involved in lawmaking and governance projects. These concerns focus on interest group capture, entrenchment of western economic elites, creeping privatization, …

Interplay Of Antibody Responses To Closely Related Viruses And Autoimmunity, Chun-Yang Lin

Theses and Dissertations (ETD)

Antibodies produced by B cells are crucial for eliminating infectious pathogens and preventing secondary infection. However, antibodies may also bind to self-antigens and contribute to autoimmune disease. While pathogen infections have been associated with development of autoimmune diseases, the precise contributions of viral infections to autoimmunity remain elusive. The presence of autoreactive antibodies is associated with an increased risk of developing autoimmune disease. Thus, analyzing the impact of viral infection and vaccination on the development of autoreactive antibodies will provide essential insight into mechanisms that contribute to autoimmune disease.

Examining How Non-Antifungal Medications Impact Host-Fungal Interactions, Parker Ryan Reitler

Theses and Dissertations (ETD)

Invasive fungal infections (IFIs) are estimated to kill 1.5 million people each year with mortality rates remaining high (around 30-70%), despite the availability of three major classes of antifungal drugs. Furthermore, millions more suffer debilitating infections of the mucosal membranes or destructive subcutaneous mycoses. One of the most common IFIs is invasive candidiasis, mostly commonly caused by Candida albicans infections, with mortality rates of approximately 30%. In addition, the population of susceptible individuals and the incidences of both mucosal and systemic mycoses continue to rise due to the widespread use of broad-spectrum antibacterial agents, cancer chemotherapies, and the immune suppressive …

Molecular Determinants Of Trp Channel Gating, Briar J. Bell

Theses and Dissertations (ETD)

Transient Receptor Potential (TRP) ion channels are a family of proteins that contribute to a myriad of physiological functions. They have been extensively studied since their discovery 35 years ago; however, there are still many questions regarding the mechanisms by which they function. This family of proteins is characterized by a generally shared structure: an intracellular N terminus featuring ankyrin repeats, six transmembrane helices including the pore domain, a re-entrant loop and TRP helix, and an intracellular C terminus with varying structure. Each of these regions has been implicated in the regular function of TRPs, while mutations that alter trafficking …

Anatomical And Physiological Characterization Of Striatal Activation Of The Cerebral Cortex, Yuhan Wang

Theses and Dissertations (ETD)

Purpose. The striatum receives excitatory glutamatergic input from nearly all parts of the cerebral cortex, and its output is delivered by direct pathway medium spiny neurons (dMSNs) expressing D1 type dopamine (DA) receptors (D1Rs) and indirect pathway MSNs (iMSNs) expressing D2 DA receptors (D2Rs). Loss of DA results in the imbalance between the activities of dMSNs and iMSNs in Parkinson’s disease (PD), leading to ab-normal interactions between the cerebral cortex and striatum. Thus, understanding the inter-actions between the cortex and striatum is important. The monosynaptic corticostriatal cir-cuit has been extensively studied. In contrast, due to the poly-synaptic nature of the …

Characterizing The Cellular Response To Astrovirus Infection In The Central Nervous System, Amy E. Davis

Theses and Dissertations (ETD)

Central nervous system (CNS) diseases caused by infectious agents pose significant health challenges, often leading to severe neurological dysfunction with high morbidity and mortality rates. Astroviruses (AstVs), traditionally known for causing gastroenteritis, have recently been identified as neuroinvasive pathogens capable of inducing neurological diseases. This study explores the cellular and molecular mechanisms by which AstV, specifically non-classical VA1 and classical HAstV1 genotypes, induce CNS pathology in human neurons and astrocytes. Utilizing primary human neurons and astrocytes from various brain regions, this research investigates the cytotoxic effects, apoptotic pathways, and inflammatory responses triggered by these viruses. This study reveals that VA1 …

Improving Health Outcomes In Children And Adolescents With Asthma, Elizabeth Kate Coffey Moore

Theses and Dissertations (ETD)

Asthma is among the most prevalent chronic illnesses found in the United States, affecting millions of people under the age of 18 each year. The disease burden for affected children, adolescents, their families, and the healthcare system is immense. Finding ways to mitigate its impact is critical to improving the lives of affected children, adolescents, and their families. The three chapters included in this dissertation aimed to document the asthma triggers that children and adolescents are exposed to and strategies for reducing them and to demonstrate using Imogene King’s conceptual framework to guide nursing practice to care for and nursing …

La Négociation De L’Altérité Aux Marges : L’Agentivité Des Aventuriers Dans Les Sauteurs Et The Land Between, Destiny Rosulme , '24

Senior Theses, Projects, and Awards

No abstract provided.

Imaginer La Vie De La Fin De L'Anthropocène Dans La Mort De La Terre, Caroline Lucas , '24

Senior Theses, Projects, and Awards

No abstract provided.

Investigating The Role Of The Mammalian Dsrnai Pathway In Preventing Infection By Respiratory Syncytial Virus And Human Coronavirus, Iliana Portelli

Theses and Dissertations (Comprehensive)

dsRNA interference (dsRNAi) is an antiviral innate immune response that uses long dsRNA (>40bp) to inhibit gene expression through the RNA interference (RNAi) pathway. dsRNAi is an important antiviral defense mechanism in plants, invertebrates, and fungi. However, its use as an antiviral mechanism in vertebrates is not well understood. Evidence proving the significance of dsRNAi in vertebrate cells does exist, but there is still a lack of research in this area, particularly with the use of long dsRNA. Respiratory Syncytial Virus (RSV) and human coronaviruses (HCoVs) are two single stranded RNA (ssRNA) viruses that infect airway cells. This study …

Speaking Back To Sexual Privacy Invasions, Brenda Dvoskin

Scholarship@WashULaw

Many big players in the internet ecosystem do not like hosting sexual expression. They often justify these bans as a protection of sexual privacy. For example, Meta states that it removes sexual imagery to prevent the nonconsensual distribution of sexual images. In response, this Article argues that banning digital sexual expression is counterproductive if the aim is to alleviate the harms inflicted by sexual privacy losses.

Contemporary sexual privacy theory, however, lacks analytical tools to explain why nudity bans harm the interests they intend to protect. This Article aims at building those tools. The main contribution is an invitation to …

Provisioning Digital Tools And Systems For Government Use, Brenda Dvoskin, Julie E. Cohen, Paul Ohm, Meg Leta Jones, Smitha Krishna Prasad, Nina-Simone Edwards

Scholarship@WashULaw

This document is part of a larger project aimed at reinventing the administrative state for effective governance of the digital, information-driven economy. It explores how the administrative state can more effectively equip itself with digital tools and systems that align with and improve government’s ability to serve public values. Established approaches to digital provisioning fail in many important respects. Among others, they introduce thorny coordination problems while doing little to ensure design for broader public values; they cause obsolete and/or poorly conceived requirements to cascade through the development process for new tools and systems; they magnify the potential for technology-driven …