Digital Commons Network^™

Inactivation Of The Ul37 Deamidase Enhances Virus Replication And Spread Of The Hsv-1(Vc2) Oncolytic Vaccine Strain And Secretion Of Gm-Csf, Carolyn M. Clark, Nithya Jambunathan, Therese M.A. Collantes, Konstantin G. Kousoulas

Faculty Publications

The HSV-1 (VC2) live-attenuated vaccine strain was engineered with specific deletions in the amino termini of glycoprotein K (gK) and membrane protein UL20, rendering the virus unable to enter neurons and establish latency. VC2 replicates efficiently in epithelial cell culture but produces lower viral titers and smaller viral plaques than its parental HSV-1 (F) wild-type virus. VC2 is an effective live-attenuated vaccine against HSV-1 and HSV-2 infections in mice and guinea pigs and an anti-tumor immunotherapeutic and oncolytic virus against melanoma and breast cancer in mouse models. Previously, we reported that the gK/UL20 complex interacts with the UL37 tegument protein, …

Comparison Of High-Resolution Naip And Unmanned Aerial Vehicle (Uav) Imagery For Natural Vegetation Communities Classification Using Machine Learning Approaches, Parth Bhatt, Ann Maclean

Michigan Tech Publications, Part 1

To map and manage forest vegetation including wetland communities, remote sensing technology has been shown to be a valid and widely employed technology. In this paper, two ecologically different study areas were evaluated using free and widely available high-resolution multispectral National Agriculture Imagery Program (NAIP) and ultra-high-resolution multispectral unmanned aerial vehicle (UAV) imagery located in the Upper Great Lakes Laurentian Mixed Forest. Three different machine learning algorithms, random forest (RF), support vector machine (SVM), and averaged neural network (avNNet), were evaluated to classify complex natural habitat communities as defined by the Michigan Natural Features Inventory. Accurate training sets were developed …

Soil Organic Matter Diagenetic State Informs Boreal Forest Ecosystem Feedbacks To Climate Change, Allison N. Myers-Pigg, Karl Kaiser, Ronald Benner, Susan E. Ziegler

Faculty Publications

The fate of soil organic carbon (SOC) in boreal forests is dependent on the integrative ecosystem response to climate change. For example, boreal forest productivity is often nitrogen (N) limited, and climate warming can enhance N cycling and primary productivity. However, the net effect of this feedback on the SOC reservoir and its longevity with climate change remain unclear due to difficulty in detecting small differences between large and variable carbon (C) fluxes needed to determine net changes in soil reservoirs. The diagenetic state of SOC – resulting from the physicochemical and biological transformations that alter the original biomolecular composition …

Arxiv Annual Report 2022, Cornell Tech

Copyright, Fair Use, Scholarly Communication, etc.

arXiv is a curated research-sharing platform open to anyone. As a pioneer in open access, arXiv.org now hosts more than 2.1 million scholarly articles in eight subject areas, curated by our strong community of volunteer moderators who balance content quality with distribution speed. arXiv provides an article submission portal, a TeX compilation service, search and discovery tools, web distribution for human readers,API access, machine readable data sets, and community-developed tools. Our emphasis on openness, collaboration, and scholarship provides the strong foundation on which arXiv thrives.

Mission — arXiv is an open platform where researchers can share and discover new, relevant, …

District Court Rejects Constitutional Challenge To Federal Hate Crime Prosecution In Anti-Lgbtq+ Bias Case, Arthur S. Leonard

Other Publications

No abstract provided.

Adoption Ouroboros: Repeating The Cycle Of Adoption As Rescue, Malinda L. Seymore

Faculty Scholarship

Ouroboros—the circular symbol of the snake eating its tail; an endless cycle. As the U.S. recently withdrew from Afghanistan in chaos and Russia invaded Ukraine, the attention of Americans turned, as it frequently has in times of international conflict, to the plight of children in need of rescue. For many Americans, rescue is synonymous with adoption. The history of international adoption began with rescues following America’s wars in Europe and Asia and continues today through other violent upheavals. International adoption is an ouroboros, repeating the pattern of adoption as a response to humanitarian crises. But as human and charitable as …

Originalism: Erasing Women From The Body Politic, Malinda L. Seymore

Faculty Scholarship

In Dobbs v. Jackson Women's Health, the Court relied on originalism to excise women from the Constitution. Originalism is purposefully backward-looking. With cherry-picked history, the Court created a future that looks to the past: a past where unwed pregnancy is shameful and can be redeemed only by secret adoption. Yet the case has revealed originalism as a flawed method, harmed the legitimacy of the Court, and energized those supporting abortion rights.

‘Chinese Virus’: A Critical Discourse Analysis Of Anti-Asian Racist Discourse During The Covid-19 Pandemic, Peiwen Wang, Theresa Catalano

Department of Teaching, Learning, and Teacher Education: Faculty Publications

Since the emergence of COVID-19, researchers have documented an increase in cases of anti-Asian racism and hate crimes. Research shows a possible connection between the ‘Chinese virus’ discourse of the Trump administration and violence in society (Arora and Kim 2020:387). Drawing from critical discourse studies we explore 2,071 comments from one YouTube video which documents anti-China rhetoric by the Trump administration in order to understand the underlying strategies commenters relied on in their reproduction and defence of this discourse. Findings show the trickle-down influence of Trump’s discourse on YouTube commenters, but also ways in which social media created a platform …

The Life Cycle Of The Neotropical Water Strider Telmatometra Withei In Different Salinity Environments, Anakena M. Castillo, Luis F. De León

Neotropical Biodiversity

No abstract provided.

Emotional Support Animal Partnerships: Behavior, Welfare, And Clinical Involvement, Jillian Ferrell, Susan L. Crowley

Psychology Student Research

The present study gathered information about the characteristics of individuals and dogs in Emotional Support Animal (ESA) partnerships, instances of service-animal misrepresentation, animal welfare and behavior, dimensions of bond quality, and health professional involvement. Seventy-seven adults (53 female, 24 male) with a canine ESA were surveyed via Qualtrics panel services. Data were analyzed for descriptive data, as well as correlational analyses among variables. Participants reported high frequencies of misrepresentation of emotional support animals, access law violations, and problematic instances including ESAs with a history of aggression and times when participants were unable to care for their dog. The majority of …

The Impact Of Place-Based Education On Middle School Students’ Environmental Literacy And Stewardship, Erica R. Hamilton, Lisa Marckini-Polk

Open Access Publishing Support Funded Articles

This article centers on the use of place-based education (PBE), an internationally known and employed pedagogy. This yearlong, mixed-methods study centers on a grant-funded program aimed at supporting secondary teachers’ implementation of environmental place-based education (PBE), specifically meaningful watershed education experiences (MWEEs). Intended outcomes of this grantbased program included increasing middle school students’ environmental stewardship capacity; expanding their Great Lakes literacy; building teachers’ capacity for implementing robust environmental PBE; and increasing teachers’ capacity for teaching Great Lakes content. Situated in the Midwestern United States, two experienced PBE teachers and their middle school students (N = 226) from two separate schools …

Inducible Nitric Oxide Synthase Embedded In Alginate/Polyethyleneimine Hydrogel As A New Platform To Explore No-Driven Modulation Of Biological Function, Shaimaa Maher, Lauren A. Smith, Celine A. El-Khoury, Haitham F. Kalil, Khalid Sossey-Alaoui, Mekki Bayachou

Chemistry Faculty Publications

Nitric oxide (NO), a small free radical molecule, turned out to be pervasive in biology and was shown to have a substantial influence on a range of biological activities, including cell growth and apoptosis. This molecule is involved in signaling and affects a number of physiologic functions. In recent decades, several processes related to cancer, such as angiogenesis, programmed cell death, infiltration, cell cycle progression, and metastasis, have been linked with nitric oxide. In addition, other parallel work showed that NO also has the potential to operate as an anti-cancer agent. As a result, it has gained attention in cancer-related …

Widespread Variation In Salt Tolerance Within Freshwater Zooplankton Species Reduces The Predictability Of Community-Level Salt Tolerance, Matthew Schuler

Department of Biology Faculty Scholarship and Creative Works

The salinization of freshwaters is a global threat to aquatic biodiversity. We quantified variation in chloride (Cl⁻) tolerance of 19 freshwater zooplankton species in four countries to answer three questions: (1) How much variation in Cl⁻ tolerance is present among populations? (2) What factors predict intraspecific variation in Cl⁻ tolerance? (3) Must we account for intraspecific variation to accurately predict community Cl⁻ tolerance? We conducted field mesocosm experiments at 16 sites and compiled acute LC50s from published laboratory studies. We found high variation in LC50s for Cl⁻ tolerance in multiple species, which, in the …

Belonging: The Heart Of Our Family Calling, Janice M. Brewer-Stevens

Doctor of Ministry

Transracial families are part of our society whether people like it or not. There are not enough adoptive families of any kind, and children need support, love, and acceptance. Last August the Census Bureau reported that the number of non-Hispanic Americans who identify as multiracial jumped by 127 percent over the last decade, and for people who identified as Hispanic, the increase was even higher. Also, from 2017 to 2019, half of all U.S. adoptions were of non-white children, and half of these adoptions were transracial. The result? An increase in transracial adoptions in the United States and the subsequent …

Choline Supplementation Alters Hippocampal Cytokine Levels In Adolescence And Adulthood In An Animal Model Of Fetal Alcohol Spectrum Disorders, Jessica A. Baker, Tamara S. Bodnar, Kristen Breit, Joanne Weinberg, Jennifer D. Thomas

Psychology Faculty Publications

Alcohol (ethanol) exposure during pregnancy can adversely affect development, with long-lasting consequences that include neuroimmune, cognitive, and behavioral dysfunction. Alcohol-induced alterations in cytokine levels in the hippocampus may contribute to abnormal cognitive and behavioral outcomes in individuals with fetal alcohol spectrum disorders (FASD). Nutritional intervention with the essential nutrient choline can improve hippocampal-dependent behavioral impairments and may also influence neuroimmune function. Thus, we examined the effects of choline supplementation on hippocampal cytokine levels in adolescent and adult rats exposed to alcohol early in development. From postnatal day (PD) 4-9 (third trimester-equivalent), Sprague-Dawley rat pups received ethanol (5.25 g/kg/day) or sham …

The Facet Of Human Impact: Solenopsis Invicta Buren, 1972 Spreading Around The Atlantic Forest, Manuela O. Ramalho, Victor H. Nagatani, Juliana M. C. Alves, Otavio G. M. Silva, Eduardo G. P. Fox, Rodrigo F. Souza, Debora Y. Kayano, Ricardo Harakava, Alexandre W. S. Hilsdorf, Maria S. C. Morini

Biology Faculty Publications

The present investigation deals with some aspects of the diversity of fire ants (Hymenoptera: Formicidae) in their native range. The Red Imported Fire Ant Solenopsis invicta is native to the tropical and subtropical inland territories of South America. In Brazil, it mainly occurs around the Pantanal region and across the Paraguay river, a region composed of grasslands which are seasonally flooded. Recent studies have evidenced this fire ant species is gradually spreading to other regions of Brazil. In the present investigation, we surveyed the molecular diversity of S. invicta populations across fragments of Atlantic Forest in Sao Paulo, Brazil, using …

Long‑Term Adaptation To Galactose As A Sole Carbon Source Selects For Mutations Outside The Canonical Gal Pathway, Artemiza A. Martínez, Andrew Conboy, Sean Buskirk, Daniel A. Marad, Gregory I. Lang

Biology Faculty Publications

Galactose is a secondary fermentable sugar that requires specific regulatory and structural genes for its assimilation, which are under catabolite repression by glucose. When glucose is absent, the catabolic repression is attenuated, and the structural GAL genes are fully activated. In Saccharomyces cerevisiae, the GAL pathway is under selection in environments where galactose is present. However, it is unclear the adaptive strategies in response to long-term propagation in galactose as a sole carbon source in laboratory evolution experiments. Here, we performed a 4,000-generation evolution experiment using 48 diploid Saccharomyces cerevisiae populations to study adaptation in galactose. We show that fitness …

Multiple Behaviors For Turning Performance Of Pacific Bluefin Tuna (Thunnus Orientalis), Abigail M. Downs, Allison Kolpas, Barbara A. Block, Frank E. Fish

Biology Faculty Publications

Tuna are known for exceptional swimming speeds, which are possible because of their thunniform lift-based propulsion, large muscle mass and rigid fusiform body. A rigid body should restrict maneuverability with regard to turn radius and turn rate. To test if turning maneuvers by the Pacific bluefin tuna (Thunnus orientalis) are constrained by rigidity, captive animals were videorecorded overhead as the animals routinely swam around a large circular tank or during feeding bouts. Turning performance was classified into three different types: (1) glide turns, where the tuna uses the caudal fin as a rudder; (2) powered turns, where the animal uses …

The Banner - V. 158, No. 2 - (February 2023), Christian Reformed Church In North America

The Banner

No abstract provided.

The Known And Unknowns Of Aphid Biotypes, And Their Role In Mediating Host Plant Defenses, Neetu Khanal, Christopher Vitek, Rupesh Kariyat

Entomology and Plant Pathology Faculty Publications and Presentations

Insect species are subjected to disparate selection pressure due to various biotic and abiotic stresses. Management practices including the heavy use of chemical insecticides and introduction of insect-resistant plant cultivars have been found to accelerate these processes. Clearly, natural selection coupled with human intervention have led to insect adaptations that alter phenotypes and genetic structure over time, producing distinct individuals with specialized traits, within the populations, commonly defined as biotypes. Biotypes are commonly found to have better fitness in the new environment and, in the case of aphids, the most commonly studied system for biotypes, have the ability to successfully …

Subclonal Evolution And Expansion Of Spatially Distinct Thy1-Positive Cells Is Associated With Recurrence In Glioblastoma, Wajd N. Al-Holou, Hanxiao Wang, Visweswaran Ravikumar, Sunita Shankar, Morgan Oneka, Ziad Fehmi, Roel Gw Verhaak, Hoon Kim, Drew Pratt, Sandra Camelo-Piragua, Corey Speers, Daniel R. Wahl, Todd Hollon, Oren Sagher, Jason A. Heth, Karin M. Muraszko, Theodore S. Lawrence, Ana C. De Carvalho, Tom Mikkelsen, Arvind Rao, Alnawaz Rehemtulla

Neurosurgery Articles

PURPOSE: Glioblastoma(GBM) is a lethal disease characterized by inevitable recurrence. Here we investigate the molecular pathways mediating resistance, with the goal of identifying novel therapeutic opportunities.

EXPERIMENTAL DESIGN: We developed a longitudinal in vivo recurrence model utilizing patient-derived explants to produce paired specimens(pre- and post-recurrence) following temozolomide(TMZ) and radiation(IR). These specimens were evaluated for treatment response and to identify gene expression pathways driving treatment resistance. Findings were clinically validated using spatial transcriptomics of human GBMs.

RESULTS: These studies reveal in replicate cohorts, a gene expression profile characterized by upregulation of mesenchymal and stem-like genes at recurrence. Analyses of clinical databases …

February 2023 Louisiana Bar Exam, Louisiana Supreme Court Committee On Bar Admissions

Louisiana Bar Exams

No abstract provided.

Clinical Risk Factors Of Adverse Outcomes Among Women With Covid-19 In The Pregnancy And Postpartum Period: A Sequential, Prospective Meta-Analysis, Emily R. Smith, Erin Oakley, Gargi Wable Grandner, Gordon Rukundo, Fouzia Farooq, Kacey Ferguson, Sasha Baumann, Kristina Adams Waldorf, Yalda Afshar, Marleen Temmerman

Obstetrics and Gynaecology, East Africa

Objective: This sequential, prospective meta-analysis (sPMA) sought to identify risk factors among pregnant and postpartum women with COVID-19 for adverse outcomes related to: disease severity, maternal morbidities, neonatal mortality and morbidity, adverse birth outcomes.

Data sources: We prospectively invited study investigators to join the sPMA via professional research networks beginning in March 2020.

Study eligibility criteria: Eligible studies included those recruiting at least 25 consecutive cases of COVID-19 in pregnancy within a defined catchment area.

Study appraisal and synthesis methods: We included individual patient data from 21 participating studies. Data quality was assessed, and harmonized variables for risk factors and …

The Role Of Salinity In Recovery Of White Sturgeon (Acipenser Transmontanus) From Stimulated Angling Stress, Ryan B. Shartau, Jacelyn Shu, Daniel W. Baker

Biology Faculty Publications and Presentations

White sturgeon (Acipenser transmontanus) in the Lower Fraser River are the focus of a catch-and-release angling fishery in British Columbia, Canada. However, the lower region of the catch area includes areas where tidal waters invade, and the consequence of salinity levels on recovery from an angling challenge are not characterized in sturgeon, despite theoretical implications of its import. We acclimated white sturgeon to various salinities (0, 10 and 20 (parts per thousand)) to investigate the effects of acclimation on recovery from stimulated angling stress that was induced through manual chasing. This challenge elicited the traditional physiological responses such as ion …

Quantifying 64 Drugs, Illicit Substances, And D- And L- Isomers In Human Oral Fluid With Liquid-Liquid Extraction, Brian Robbins, Rob E. Carpenter, Mary Long, Jacob Perry

Human Resource Development Faculty Publications and Presentations

Although human oral fluid has become more routine for quantitative drug detection in pain management, detecting a large scope of medications and substances is costly and technically challenging for laboratories. This paper presents a quantitative assay for 64 pain medications, illicit substances, and drug metabolites in human oral fluid. The novelty of this assay is that it was developed on an older model AB SCIEX 4000 instrument and renders obscure the need for more technical and expensive laboratory equipment. This method includes addition of internal standard and a 2-step liquid-liquid extraction and dry-down step to concentrate and clean the samples. …

Teaching Learning Newsletter Vol 3 Issue 1, Sastra Deemed To Be University

TLC Newsletter

No abstract provided.

Columns Spring 2023, Southern Adventist University

Alumni Newsletter

Features information about early childhood education, small business endeavors, service opportunities, and the dedication of land for the future School of Business facility.

Hemoglobin Concentration Impacts Viscoelastic Hemostatic Assays In Icu Admitted Patients, David J Roh, Tiffany R Chang, Aditya Kumar, Devin Burke, Glenda Torres, Katherine Xu, Winni Yang, Azzurra Cottarelli, Ernest Moore, Angela Sauaia, Kirk Hansen, Angela Velazquez, Amelia Boehme, Athina Vrosgou, Shivani Ghoshal, Soojin Park, Sachin Agarwal, Jan Claassen, E Sander Connolly, Gebhard Wagener, Richard O Francis, Eldad Hod

Faculty, Staff and Student Publications

Objectives: Low hemoglobin concentration impairs clinical hemostasis across several diseases. It is unclear whether hemoglobin impacts laboratory functional coagulation assessments. We evaluated the relationship of hemoglobin concentration on viscoelastic hemostatic assays in intracerebral hemorrhage (ICH) and perioperative patients admitted to an ICU.

Design: Observational cohort study and separate in vitro laboratory study.

Setting: Multicenter tertiary referral ICUs.

Patients: Two acute ICH cohorts receiving distinct testing modalities: rotational thromboelastometry (ROTEM) and thromboelastography (TEG), and a third surgical ICU cohort receiving ROTEM were evaluated to assess the generalizability of findings across disease processes and testing platforms. A separate in vitro ROTEM laboratory …

Biological Correlates Of The Effects Of Auricular Point Acupressure On Pain, Chao Hsing Yeh, Nada Lukkahatai, Xinran Huang, Hulin Wu, Hongyu Wang, Jingyu Zhang, Xinyi Sun, Thomas J Smith

Faculty, Staff and Student Publications

BACKGROUND: To identify candidate inflammatory biomarkers for the underlying mechanism of auricular point acupressure (APA) on pain relief and examine the correlations among pain intensity, interference, and inflammatory biomarkers.

DESIGN: This is a secondary data analysis.

METHODS: Data on inflammatory biomarkers collected via blood samples and patient self-reported pain intensity and interference from three pilot studies (chronic low back pain, n = 61; arthralgia related to aromatase inhibitors, n = 20; and chemotherapy-induced neuropathy, n = 15) were integrated and analyzed. This paper reports the results based on within-subject treatment effects (change in scores from pre- to post-APA intervention) for …

Alphaviruses Detected In Mosquitoes In The North-Eastern Regions Of South Africa, 2014 To 2018, Milehna M Guarido, Isabel Fourie, Kgothatso Meno, Adriano Mendes, Megan A Riddin, Caitlin Macintyre, Sontaga Manyana, Todd Johnson, Maarten Schrama, Erin E Gorsich, Basil D Brooke, Antonio Paulo G Almeida, Marietjie Venter

Faculty, Staff and Student Publications

The prevalence and distribution of African alphaviruses such as chikungunya have increased in recent years. Therefore, a better understanding of the local distribution of alphaviruses in vectors across the African continent is important. Here, entomological surveillance was performed from 2014 to 2018 at selected sites in north-eastern parts of South Africa where alphaviruses have been identified during outbreaks in humans and animals in the past. Mosquitoes were collected using a net, CDC-light, and BG-traps. An alphavirus genus-specific nested RT-PCR was used for screening, and positive pools were confirmed by sequencing and phylogenetic analysis. We collected 64,603 mosquitoes from 11 genera, …