Digital Commons Network^™

Optimization Of Vacuum Tumbling Combined Ultra High-Pressure Treatment Preparation Of Conditioned Chicken Breast, Xu Mei-Yu, He Dan, Song Hong-Bo, Tang Dao-Bang, Huang Qun

Food and Machinery

Water retention rate and cooking loss rate were used as the evaluation index. To optimize the proportion of low phosphorus water retaining agent and the technological conditions of conditioning chicken breast prepared by vacuum tumble-ultra-high pressure, orthogonal test and response surface analysis were used respectively, and the texture indexes of the treated chicken breast were measured. The results showed that the optimal low-phosphorus water-retaining agent were formulated as follows: sodium pyrophosphate 0.2%, starch 7.0%, and sodium bicarbonate 1.5%. The optimized conditions of vacuum rolling ultra high-pressure process were ultra-high pressure processing for 10 min with, pressure 190 MPa after a …

Research Progress On The Mechanism Of Uric Acid-Lowering Natural Bioactive Compounds, Jiang Lin-Hong, Yang Xi-Hong, Xie Jing-Wen, Xie Wan-Cui

Food and Machinery

Based on purine metabolism and the pathway of uric acid, four kinds of uric acid-lowering modes and mechanisms of natural bioactive compounds were summarized, mainly analyzed the uric acid-lowering modes and research results of natural extracts, protein enzymatic hydrolysates, polypeptides, probiotics and other active compounds. It is provided guidance for the development of medicinal resources, medical auxiliary food and natural anti-gout drugs.

Effect Of L-Cysteine Hydrochloride On The Contents Of Four Toxic Aldehydes And Fluorescent Ages In Biscuits, Ting Weng, Jie Zheng, Cai-Huan Huang, Shi-Yi Ou

Food and Machinery

In the current study, different levels of L-cysteine hydrochloride (0.3, 0.6, 1.0, 1.5 g/kg) were incorporated in dough for the preparation of biscuits to investigate the effect of L-cysteine on the appearance and the content of four toxic aldehydes and fluorescent advanced glycation end products(AGEs) in biscuits. The results showed that the addition of L-cysteine hydrochloride brightened the color of the biscuits, and cause a slight decrease in pH values. The incorporation of L-cysteine hydrochloride significantly decreased the content of 5-hydroxymethyl furfural(HMF), 3-deoxyglucosone(3-DG), glyoxal(GO), methylglyoxal(MGO) and fluorescent AGEs in biscuits in a dose-dependent manner. The addition of L-cysteine at the …

Preparation And Structural Characterization Of Zinc-Binding Antioxidant Peptides From Corbicula Fluminea Hydrolysate, Jing-Jing Liu, Yun-Tao Xu, Chen-Hui Zhu, Qian-Qian Huang, Yao-Ping Han, Xue-Feng Wang, Ran Zhang, Yang-Jun Dai

Food and Machinery

Using clam meat as protein source, the hydrolysate was obtained by enzymehydrolysis with neutral protease. Antioxidant peptides were then separated and purified from the enzymatich-ydrolysate of Corbicula flumineaby using glucose gel G-50. Zinc-binding antioxidant peptides were prepared according to optimal process. Qualitative analysis of chelate products was carried out byninhydrin method and sodium sulfide method. Ultraviolet spectra, Fourier infrared and fluorescent light spectra, X-radiation diffraction and scanning electron microscopy were applied to predict the structure of the chelate products. The results showed that the product powder is a kind of polypeptide zinc chelate. The carboxyl, amino and amide bonds of …

The Effects Of High-Pressure Microfluidization On The Properties Of Lotus Seed Starch-Glycerin Monostearin Complex, Yi-Xin Zheng, Ze-Bin Guo, Yi Zhang, Bao-Dong Zheng, Shao-Xiao Zeng, Hong-Liang Zeng

Food and Machinery

In order to investigate the effects of microfluidization in different pressure combined with pullulanase debranching treatment on the structural properties and starch composition of lotus seed starch-glycerin monostearin complex, the determination of amylose, complex index, crystal structure, microstructural morphology and starch composition were discussed and analyzed. The results showed that the appropriate microfluidization conditions were more conducive to exert the debranching of pullulanase and facilitate the formation of lotus seed starch-glycerin monostearin complex. 80 MPa of microfluidization condition contributed to the crystal structure of the starch complex developing in the direction of high-microcrystalline V_6I-type, and the formation of …

Breeding Of Yarrowia Lipolytica With High Lipase Production By Atmospheric Room Temperature Plasma Mutagenesis, Ya-Mei Ji, Li-Mei Wang, Bin Qi

Food and Machinery

A lipase-producing Yarrowia lipolytica strain YL1 was mutagenized by atmospheric room temperature plasma. Tributyrin plate method, p-NPP method and acid-base titration method were used to screen out the target strain C4 with high lipase production, and then its genetic stability was studied. The results showed that the optimal mutagenesis time of Y. lipolytica strain YL₁ was 60 s, and the lethality rate of the strain reached 97.45%. The lipase activity of the mutant strain C4 was 13.4 U/mL, which was 82.6% higher than the original strain, and it was genetically stable after multiple generations of culture. Compared with the …

Effect Of Extraction Method On The Functional Characteristics Of Leaf Protein From Green Leaves Ramie, Shi-Qi Zhi, Man-Sheng Wang, Feng-Ling Ye, Long Chen, Xiao-Ting Zhang, Hao-Nan Qiu, Qiang He, Xia-Yu Xiong, Yi Dong

Food and Machinery

In this paper, 3 kinds of methods such as ultrasonic assisted alkali solution extraction, salt solution extraction and water extraction, were used to extract the green leaf ramie protein, and the protein was also analyzed on the protein concentration, nitrogen solubility index, foaming property, foaming stability, emulsifying property and emulsifying stability. Results: The protein extracted with ultrasonic assisted salt solution exhibited the highest protein concentration (10.73 mg/mL) and nitrogen dissolution index (61.95%). Additionally, the foaming property (26.34%) and foam stability (24.49%) of the protein extracted by this method were significantly better than the other two extraction methods. The protein extracted …

Progress In Saccharomyces Cerevisiae Genome Research And Relative Application, Bi-Yan Chen, Li Wen

Food and Machinery

No abstract provided.

Saliva As A Diagnostic Tool To Measure Polycyclic Aromatic Hydrocarbon Exposure In Dental Patients Exposed To Intimate Partner Violence (Ipv), Aramandla Ramesh, Leslie R. Halpern, Janet H. Southerland, Samuel E. Adunyah, Pandu R. Gangula

School of Dentistry Faculty Publications

Background: Social habits such as tobacco use, alcohol consumption, and chemically contaminated diet contribute to poor oral health. Intimate Partner Violence (IPV) is a global public health epidemic which can exacerbate the prevalence of health conditions affecting a victim's lifespan. This study investigates using saliva as a biomarker for detecting levels of benzo(a)pyrene [B(a)P]; a toxicant present in cigarette smoke and barbecued meat in a population of IPV + female patients. Methods: A cross-sectional IRB-approved study utilized 63 female participants (37 African Americans [AA], and 26 non-African Americans [NAA]), who provided consent for the study. Participants submitted samples of saliva, …

Mitigation Of Salmonella On Food Contact Surfaces By Using Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba), Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich

Department of Food Science and Technology: Faculty Publications

Contaminated surfaces can transmit pathogens to food in industrial and domestic food-handling environments. Exposure to pathogens on food contact surfaces may take place via the cross-contamination of pathogens during postprocessing activities. Formaldehyde-based commercial sanitizers in recent years are less commonly being used within food manufacturing facilities due to consumer perception and labeling concerns. There is interest in investigating clean-label, food-safe components for use on food contact surfaces to mitigate contamination from pathogenic bacteria, including Salmonella. In this study, the antimicrobial effects of two types of organic acid mixtures containing 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), Activate DA™ and Activate US WD-MAX™, …

Mitigation Of Salmonella On Food Contact Surfaces By Using Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba), Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich

Department of Food Science and Technology: Faculty Publications

Contaminated surfaces can transmit pathogens to food in industrial and domestic food-handling environments. Exposure to pathogens on food contact surfaces may take place via the cross-contamination of pathogens during postprocessing activities. Formaldehyde-based commercial sanitizers in recent years are less commonly being used within food manufacturing facilities due to consumer perception and labeling concerns. There is interest in investigating clean-label, food-safe components for use on food contact surfaces to mitigate contamination from pathogenic bacteria, including Salmonella. In this study, the antimicrobial effects of two types of organic acid mixtures containing 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), Activate DA™ and Activate US WDMAX …

Drosophila Model For Potential Plasticizer Induced Hyperactivity, Xavier Hayes, Erika L. Doctor, Cassandra S. Korte, Kimberly D. Rowland

Faculty and Staff Publications & Presentations

Plasticizing additives such as phthalates, are known to cause disruptions in human nervous systems linked to neurological disorders, thus with the Drosophila activity monitoring (DAM) system test can be conducted to consider whether the flies are exhibiting hyperactivity (Hlisníková et al., 2021). Drosophila is a useful model for exposure of metabolic compounds followed by general and specific assays of their effects. Our experiments propose a link between exposure to phthalates and hyperactivity between humans that can be investigated with flies as a model for testing hyperactivity (Praveena et al., 2020). The link that is proposed is a cross-sectional study that …

Resilience And Mental Health In College Students, Indyah Ferrouillet, Bemsimbom Nkuo, Daniel Ceccoli, Patrick J. Cooper

Faculty and Staff Publications & Presentations

Research suggests a moderate incidence of depression, anxiety, and especially stress among college students (Ramon-Arbues et al., 2020). It is reasonable to believe that these mental health outcomes might be inversely correlated with resilience, the ability to adapt to new challenges.

In this study, we hypothesize an inverse correlation between resilience (as measured by the selfreported BRS-6) and stress, anxiety, and depression (as measured by the DASS-21). In other words, college students who feel resilient will experience greater mental health and wellness.

To replicate previous research that suggests these effects in females only (Ahmed & Julius, 2015), biological sex differences …

The Coronavirus Disease 2019 Pandemic Unmasked The Challenges Faced By Early-Stage Faculty In Infectious Diseases: A Call To Action, Erin M Scherer, Martin Backer, Karen Carvajal, Lara Danziger-Isakov, Sharon Frey, Leigh M Howard, Felicia Scaggs Huang, Angelica C Kottkamp, Tara Reid, Maria C Rodriguez-Barradas, Helen C Stankiewicz Karita, Zheyi Teoh, Anna Wald, Jennifer Whitaker, Zanthia Wiley, Igho Ofotokun, Kathryn M Edwards, Infectious Diseases Clinical Research Consortium (Idcrc) Mentorship Program Writing Group

Faculty, Staff and Students Publications

The coronavirus disease 2019 (COVID-19) pandemic and associated increase in family care responsibilities resulted in unsustainable personal and professional workloads for infectious diseases (ID) faculty on the front lines. This was especially true for early-stage faculty (ESF), many of whom had caregiving responsibilities. In addition, female faculty, underrepresented in medicine and science faculty and particularly ESF, experienced marked declines in research productivity, which significantly impacts career trajectories. When combined with staffing shortages due to an aging workforce and suboptimal recruitment and retention in ID, these work-life imbalances have brought the field to an inflection point. We propose actionable recommendations and …

Best Practices For Creating And Maintaining High-Impact Student Organizations, Emily Miranda, Alyssa Brule, Patrick J. Cooper

Faculty and Staff Publications & Presentations

College students often overlook, or are unaware of, the opportunities provided by their university outside of the classroom. Most universities offer extensive resources and programs that supplement students’ academic education, often in the form of student-run clubs, honor societies, and other organizations. Holding an official position or immersing yourself into new academically social situations helps to eliminate the boundaries of reaching future success in your career.

Research suggests that participating in organizational groups enhances overall personal autonomy, understanding of group interests and dynamics, and other nonacademic decision-making (Moore et al., 1998). For that reason, students should connect and participate in …

Braf V600e-Mutant Cancers Treated With Vemurafenib Alone Or In Combination With Everolimus, Sorafenib, Or Crizotinib Or With Paclitaxel And Carboplatin (Vem-Plus) Study, Blessie Elizabeth Nelson, Jason Roszik, Filip Janku, David S Hong, Shumei Kato, Aung Naing, Sarina Piha-Paul, Siqing Fu, Apostolia Tsimberidou, Maria Cabanillas, Naifa Lamki Busaidy, Milind Javle, Lauren Averett Byers, John V Heymach, Funda Meric-Bernstam, Vivek Subbiah

Faculty, Staff and Student Publications

Combined BRAF + MEK inhibition is FDA approved for BRAF V600E-mutant solid tumors except for colorectal cancer. However, beyond MAPK mediated resistance several other mechanisms of resistance such as activation of CRAF, ARAF, MET, P13K/AKT/mTOR pathway exist among other complex pathways. In the VEM-PLUS study, we performed a pooled analysis of four phase one studies evaluating the safety and efficacy of vemurafenib monotherapy and vemurafenib combined with targeted therapies (sorafenib, crizotinib, or everolimus) or carboplatin plus paclitaxel in advanced solid tumors harboring BRAF V600 mutations. When vemurafenib monotherapy was compared with the combination regimens, no significant differences in OS or …

Vitamin D Association With Renal Health And Filtration In Healthy Individuals Free Of Cardiometabolic Diseases: A Pilot Study, Meena K. Meiyyappan, Ricardo Torres, Tomas Chapman-Lopez, Keri Collins, Bayley Humphrey, Andrew Gallucci, Brent Dragoo, Leslee Funderburk, Panagiotis Koutakis, Jeff S. Forsse

International Journal of Exercise Science: Conference Proceedings

The effect of vitamin D (VITD) on bone, muscle, and over health is well know in renal failure and chronic kidney disease (CKD). However, the influence of VITD on renal health and filtration (RHF) in healthy individuals is unclear. Currently, only serum creatinine concentration (sCr) methods are used to assess renal status in health individuals. However, newer biomarkers like serum Cystatin C (CyC) and urine epidermal growth factor (uEGF) show promise in evaluating baseline RHF. The impact of Vitamin D on filtration in healthy individuals of various ages is still unknown. PURPOSE: To determine the impact of VITD on RHF …

The Usage Of Skeletal Muscle Oxygenation And Heart Rate Variability As Predictors Of Aerobic Fitness., Ricardo Torres, Tomas Chapman-Lopez, Catherine Lowry, Chris Wynveen, Panagiotis Koutakis, Jeff S. Forsse

International Journal of Exercise Science: Conference Proceedings

Heart rate variability (HRV) is used to assess the autonomic nervous system’s (ANS) activity on the heart, while skeletal muscle oxygenation (SmO2) measures how well muscles uptake oxygen from the blood. Both measurements have demonstrated strong associations with cardiorespiratory fitness and are altered with increased exercise workloads. Both have been used to assess athletic performance. While the gold standard for assessing cardiorespiratory fitness is VO2 max testing, several situations preclude the usage of a true VO2 max. Purpose: To determine if HRV and SmO2 possess predictive qualities to accurately assess cardiorespiratory fitness levels. Methods: Thirty-six healthy fit individuals (n = …

Comparison Of Ultrastructural Alterations In Peripheral Artery Disease Skeletal Muscle, Dylan T. Wilburn, Emma Fletcher, Dimitrios Miserlis, Bernd Zechmann, Panagiotis Koutakis

International Journal of Exercise Science: Conference Proceedings

Peripheral artery disease (PAD) is characterized by obstructed hemodynamics and claudication reducing quality of life and muscle function. A myopathy has been shown to develop in PAD patients and characterization of changes in skeletal muscle needs further elucidation. PURPOSE: To assess myofibrillar ultrastructural changes between control and stage IV PAD patients. METHODS: Twenty-six participants (13 control:13 stage IV) were recruited to take part in this cross-sectional study. The mean(±SD) age, mass, height, and BMI were 53(±11) years, 81(±22) kg, 165(±15) cm, and 30(±1.5) kg/m². Muscle samples were collected from the gastrocnemius and prepared for transmission electron microscopy. Relative …

Selective Inhibition Of Ribosomal Biogenesis Over Cap-Dependent Translation By Rapamycin In L6 Myotubes, Selina Uranga, James D. Fluckey

International Journal of Exercise Science: Conference Proceedings

The mechanistic target of rapamycin, or mTOR, lies at the center of skeletal muscle anabolic regulation. The mTOR kinase has at least two complexes that aide in carrying out responses to anabolic signals. Complex 1 (mTORC1) activation leads to cap-dependent translation and ribosomal biogenesis via phosphorylation of its two downstream targets 4EBP1 and P70S6K respectively. mTORC1 is inhibited by rapamycin while mTORC2 is not; however, cap-dependent translation via mTORC1 may still occur under rapamycin inhibition. PURPOSE: To identify timing and mechanistic pathway by which selective skeletal muscle anabolism continues in the presence of rapamycin inhibition. METHODS: Murine L6 …

Role Of Ghrelin Receptor In Sarcopenia: Involvement Of Redox Signaling And Rankl., Mariam A. Othman, Joo Hyun Kim, Khaled Moustfa, John Lawler

International Journal of Exercise Science: Conference Proceedings

Sarcopenia is aging-induced debilitating loss of skeletal muscle strength and function. Evidence from aging research suggests an integration of disrupted mechanotransduction and oxidative stress elevation that leads to muscle atrophy. Ghrelin is a 28 amino acid peptide hormone circulating in both acylated (AG) and unacylated (UAG) forms that acts as a nutrient sensor and metabolic regulator. Acylated ghrelin then binds to the growth hormone secretagogue receptor (GHS-R) or the “ghrelin receptor.” Emerging evidence indicates that GHS-R knockout may hold greater promise in protecting against sarcopenia and fasting-induced atrophy (Wu 2020, Sun 2020). Furthermore, ghrelin can promote the elevation of nNOS, …

The Rankl And Nox2 Signaling In The Duchenne Muscular Dystrophy Models Of Skeletal Muscle, Joo Hyun Kim, Mariam Othman, Khaled Moustafa, John Michael Lawler

International Journal of Exercise Science: Conference Proceedings

There is a severe sort of muscle disorder and dysfunction caused by a mutation of the dystrophin gene which is called Duchenne muscular dystrophy (DMD). The establishment of pathology in the limbs, respiratory systems, and hearts of DMD patients is the awareness of the main point for the inflammation and elevation of oxidative stress. NADPH oxidase-2 (Nox2) produces reactive oxygen species in response to skeletal muscle contractions. Recent studies show that various myopathy indicated the key stress response proteins which can modulate Nox2. The TRAF 6 and RANKL lead to Nox2 complex migration and construction. An increase of pro-inflammatory proteins …

Autophagy Is Required For Mtor-Mediated Anabolism In Skeletal Muscle, Patrick Ryan, Sean Stanelle, Megan Humphries, Colleen L. O'Reilly, Selina Uranga, Jessica M. Cardin, James D. Fluckey

International Journal of Exercise Science: Conference Proceedings

PURPOSE: While much has been discovered about the role autophagy in protein degradation, recent evidence suggests that autophagy is required for muscular adaptations to exercise, hinting at a hitherto unknown cross-talk between autophagic proteolysis and muscle protein anabolism. Here, we set out to further elucidate the metabolic mechanisms by which autophagy may influence protein anabolism. METHODS: L6 myoblasts received either electrical pulse stimulation (EPS) to induce muscle contraction or were unstimulated to serve as controls, and were then treated with an inhibitor of the ATG4 enzyme which catalyzes the initial step of autophagy NSC185058 (NSC, 100 μM) or DMSO as …

Randomized Pilot Study Examining The Impact Of An Online, Non-Restrictive Diet Among Working Adults, Diana Kolb, Stephanie Silveira Dr., Ec Synkowski

International Journal of Exercise Science: Conference Proceedings

There is a growing interest in health promotion emphasizing diet quality, specifically increased intake of fruits and vegetables, as a more effective approach for improving health outcomes. Recent studies examined ways to decrease fatigue in the workplace, some showing improved diet quality helps performance in the work environment. PURPOSE: This randomized pilot study aimed to test an online, non-restrictive diet among a sample of full-time employees at the University of North Texas. The primary research question asked if the diet program improved general health indicators: body fat percentage(BF%), body weight, and waist circumference(WC). METHODS: Twenty-three participants were randomized …

Gendered Dietary Supplements: Does The Marketing Reflect Different Formulations?, Alexandra P. Brojanac, Grant M. Tinsley, Madelin R. Siedler

International Journal of Exercise Science: Conference Proceedings

Many dietary supplements are marketed with gendered terms, such as “for her” and “for him.” However, whether these statements reflect different nutrient contents of products and a biological basis has not been systematically examined. PURPOSE: The purpose of this analysis was to compare the micronutrient content of dietary supplements that are sold in separate forms based on gendered marketing. METHODS: The National Institutes of Health Dietary Supplements Label Database (DSLD) was searched using gendered terms, such as “hers,” “her,” “women,” “his,” “him,” and “men.” Eighty-nine pairs of micronutrient-containing products that were commercially available as a women’s version and a men’s …

Effect Of Residual Sugar In High Sugar Grass Silages On Aerobic Stability, G. Pahlow, R. J. Merry, P. O'Kiely, T. M. Pauly, J. M. Greef

IGC Proceedings (1977-2023)

New varieties of Lolium perenne, bred for high sugar content, can contain up to 30% of water soluble carbohydrates (WSC). Only a fraction of such high contents are metabolised during a normal fermentation and the high residual sugar content (RSC) of these silages can improve the efficiency of use of nitrogen by ruminants. However, these RSC at opening for feed-out could be preferentially metabolised relative to fermentation products by all aerobically growing fungi and bacteria present on the forage. A high RSC thus can increase the risk of aerobic deterioration over that of extensively fermented silages, containing predominantly organic …

Recent Developments In Methods To Characterise The Chemical And Biological Parameters Of Grass Silage, R. S. Park, R. E. Agnew, M. G. Porter

IGC Proceedings (1977-2023)

Key points

1. Chemical analysis of forages is expensive, time consuming, environmentally unfriendly and relates poorly to the feed value for production purposes.
2. In vivo characterisation of animal feed is not a feasible option in terms of cost and analysis time.
3. NIRS is a rapid, non destructive, environmentally friendly, multi-analytical technique which can estimate the nutritive value of the feed.
4. NIRS predictive equations developed on a master instrument can be transferred to local and international sites.
5. Future assessment of forages necessitates rapid, stable, instrumentation for ‘in field’ studies.

The Effect Of Chop Length And Additive On Silage Intake And Milk Production In Cows, V. Toivonen, T. Heikkilä

IGC Proceedings (1977-2023)

Effects of reduced silage chop length on silage intake and milk production by dairy cows have been variable. Chopping of grass at harvest generally improves silage fermentation quality and consequently intake. The objective of this study was to assess the effect of chopping flail harvested silage ensiled with acid or biological additive prior to feeding on intake, milk yield and milk composition in dairy cows.

Evaluation Of Narrow-Row Forage Maize In Field-Scale Studies, W. J. Cox, J. H. Cherney, D. J. R. Cherney

IGC Proceedings (1977-2023)

Some dairy producers in the north-eastern USA adopted narrow row (0.38 m) maize forage production in the mid-1990s because of its 5% dry matter (DM) yield advantage (Cox et al., 1998). These dairy producers, however, continued to plant forage maize at high plant densities (125,000 plants/ha) under high N fertility (225 kg N/ha), despite research that indicated that forage maize had optimum DM yields and forage quality when planted at the recommended 100,000 plants/ha under 175 kg/ha of N fertility (Cox & Cherney, 2001). We evaluated forage maize at 0.38 and 0.76 m (conventional) row spacing under recommended vs. …

The Feeding Value Of Conserved Whole-Crop Wheat And Forage Maize Relative To Grass Silage And Ad-Libitum Concentrates For Beef Cattle, K. Walsh, P. O'Kiely, F. O'Mara

IGC Proceedings (1977-2023)

Grass is the predominant forage ensiled in Ireland. However, the relatively modest yields achieved in a single harvest allied to variability in digestibility and ensilability (and thus in intake and animal performance response) and the likelihood of effluent production create disadvantages for grass silage compared to the potential of some alternative forage crops. Thus, alternative forages are worthy of consideration on many farms. The objectives of this study were to quantify the relative intake, digestibility and performance of beef cattle offered grass silage, forage maize silage and whole-crop wheat (fermented or urea-treated), rank these relative to cattle offered an ad …