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Effects Of Two Different Chopping Lengths Of Maize Silage On Silage Quality And Dairy Performance, K. Mahlkow, J. Thaysen

IGC Proceedings (1977-2023)

Maize silage harvested at dough stage contains less fibre structure than grass silage. Due to higher proportions of maize silage in dairy rations and due to the fact that with longer chopping length of maize silage the contribution of fibre structure increases. The objective of this study was to assess the effect of two chopping lengths in the silage on animal performance with respect to feed intake, milk yields and ingredients as well as physiological effects of digestion.

Effect Of Additives At Harvest On The Digestibility In Lambs Of Whole Crop Barley Or Wheat Silage, S. Muhonen, I. Olsson, P. Lingvall

IGC Proceedings (1977-2023)

There are very few published articles about how silage additives affect digestibility of whole crop silage. In this experiment, male lambs were given whole crop barley or wheat silage harvested at dough stage with a number of different acid-based and bacterial additives.

Use Of Silage Additives In Ensiling Of Whole-Crop Barley And Wheat - A Comparison Of Round Big Bales And Precision Chopped Silages, M. Knický, P. Lingvall

IGC Proceedings (1977-2023)

An increasing use of whole-crop cereals, as supplementary feed, has increased interest in development of efficient ways of preserving these forages to achieve a high hygienic quality. It is known that ensiling of whole-cereals often results in silages with high concentrations of butyric acid (Weissbach & Haacker, 1988). Furthermore, problems with poor aerobic stability still persist despite the use of lactic acid bacteria (Filya et al., 2000). Therefore, the objective of this study was to examine the effect of different types of additive mixtures on the fermentation process and aerobic stability of precision chopped and baled silages.

The Fierce Green Fire: Vol. 13 Issue 12, Wofford College. Department Of Environmental Studies

The Fierce Green Fire

No abstract provided.

Medical School Watercooler Newsletter - February 17, 2023, Lindsey Lyle

Watercooler Newsletter

This is the February 17, 2023 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents include:

• Inabnet to present Fazier / Leigh Memorial Lectures
• Faculty development to focus on conflict resolution
• Teaching kitchen provides hands-on culinary medicine experience for medical students
• Menger invited to present at international symposium
• Pierce presents talk on HPV, cervical cancer
• Solidarity Bingo winners announced for solidarity week
• Save the date: USA Neuroscience Symposium
• Distinguished Scientists seminar set for Feb. 16
• USA researchers uncover novel mechanism that supports survival of pancreatic cancel cells

New Nsaid Conjugates As Potent And Selective Cox-2 Inhibitors: Synthesis, Molecular Modeling And Biological Investigation, Riham M. Bokhtia, Siva S. Panda, Adel S. Girgis, Nermin Samir

Faculty Journal Articles

No abstract provided.

Widespread Perturbation Of Ets Factor Binding Sites In Cancer., Sebastian Carrasco Pro, Heather Hook, David Bray, Daniel Berenzy, Devlin Moyer, Meimei Yin, Adam Thomas Labadorf, Ryan Tewhey, Trevor Siggers, Juan Ignacio Fuxman Bass

Faculty Research 2023

Although >90% of somatic mutations reside in non-coding regions, few have been reported as cancer drivers. To predict driver non-coding variants (NCVs), we present a transcription factor (TF)-aware burden test based on a model of coherent TF function in promoters. We apply this test to NCVs from the Pan-Cancer Analysis of Whole Genomes cohort and predict 2555 driver NCVs in the promoters of 813 genes across 20 cancer types. These genes are enriched in cancer-related gene ontologies, essential genes, and genes associated with cancer prognosis. We find that 765 candidate driver NCVs alter transcriptional activity, 510 lead to differential binding …

A Quantitative Evaluation Of Topological Motifs And Their Coupling In Gene Circuit State Distributions., Benjamin Clauss, Mingyang Lu

Faculty Research 2023

One of the major challenges in biology is to understand how gene interactions collaborate to determine overall functions of biological systems. Here, we present a new computational framework that enables systematic, high-throughput, and quantitative evaluation of how small transcriptional regulatory circuit motifs, and their coupling, contribute to functions of a dynamical biological system. We illustrate how this approach can be applied to identify four-node gene circuits, circuit motifs, and motif coupling responsible for various gene expression state distributions, including those derived from single-cell RNA sequencing data. We also identify seven major classes of four-node circuits from clustering analysis of state …

Hector Sanchez-Escandon V. Attorney General United States

2023 Decisions

Agency

Acetylcholinesterase Activity Staining In Freshwater Planarians, Christina Rabeler, Taixi Gong , '24, Danielle Ireland, O. Cochet-Escartin, Eva-Maria S. Collins

Biology Faculty Works

The serine hydrolase acetylcholinesterase (AChE) is an important neuronal enzyme which catalyzes the hydrolysis of the neurotransmitter acetylcholine and other choline esters. The breakdown of acetylcholine by AChE terminates synaptic transmission and regulates neuromuscular communication. AChE inhibition is a common mode of action of various insecticides, such as carbamates and organophosphorus pesticides. Freshwater planarians, especially the species Dugesia japonica, have been shown to possess AChE activity and to be a suitable alternative model for studying the effects of pesticides in vivo. AChE activity can be quantified in homogenates using the Ellman assay. However, this biochemical assay requires specialized equipment …

Marilyn Withrow, ’71, Donates Gift To Purchase Chemistry And Microbiology Equipment, Office Of University Communications

Gardner-Webb NewsCenter Archive

Alumna Marilyn Withrow, ’71, has made a generous gift to enhance the Gardner-Webb University chemistry and microbiology programs. This multi-year commitment provides advanced equipment and protects the investment by including funds for maintenance and upkeep.

A Data-Driven Optimization Method For Coarse-Graining Gene Regulatory Networks., Cristian Caranica, Mingyang Lu

Faculty Research 2023

One major challenge in systems biology is to understand how various genes in a gene regulatory network (GRN) collectively perform their functions and control network dynamics. This task becomes extremely hard to tackle in the case of large networks with hundreds of genes and edges, many of which have redundant regulatory roles and functions. The existing methods for model reduction usually require the detailed mathematical description of dynamical systems and their corresponding kinetic parameters, which are often not available. Here, we present a data-driven method for coarse-graining large GRNs, named SacoGraci, using ensemble-based mathematical modeling, dimensionality reduction, and gene circuit …

Was Kaposi’S Sarcoma-Associated Herpesvirus Introduced Into China Via The Ancient Silk Road? An Evolutionary Perspective, Zhenqiu Liu, Qiwen Fang, Jialu Zuo, Veenu Minhas, Charles Wood, Na He, Tiejun Zhang

Nebraska Center for Virology: Faculty Publications

Kaposi’s sarcoma-associated herpesvirus (KSHV) has become widely dispersed worldwide since it was first reported in 1994, but the seroprevalence of KSHV varies geographically. KSHV is relatively ubiquitous in Mediterranean areas and the Xinjiang Uygur Autonomous Region, China. The origin of KSHV has long been puzzling. In the present study, we collected and analysed 154 KSHV ORF-K1 sequences obtained from samples originating from Xinjiang, Italy, Greece, Iran and southern Siberia using Bayesian evolutionary analysis in BEAST to test the hypothesis that KSHV was introduced into Xinjiang via the ancient Silk Road. According to the phylogenetic analysis, 72 sequences were subtype A …

Art Students Explore Curative Plants And Botanical Research In The South, Shannon Thomason

Browse All News

No abstract provided.

Conditioned Place Avoidance Is Associated With A Distinct Hippocampal Phenotype, Partly Preserved Pattern Separation, And Reduced Reactive Oxygen Species Production After Stress, D. Parker Kelley, Lucas Albrechet-Souza, Shealan Cruise, Rajani Maiya, Aspasia Destouni, Siva S.V.P. Sakamuri, Alexander Duplooy, Meghan Hibicke, Charles Nichols, Prasad V.G. Katakam, Nicholas W. Gilpin, Joseph Francis

School of Medicine Faculty Publications

Stress is associated with contextual memory deficits, which may mediate avoidance of trauma-associated contexts in posttraumatic stress disorder. These deficits may emerge from impaired pattern separation, the independent representation of similar experiences by the dentate gyrus-Cornu Ammonis 3 (DG-CA3) circuit of the dorsal hippocampus, which allows for appropriate behavioral responses to specific environmental stimuli. Neurogenesis in the DG is controlled by mitochondrial reactive oxygen species (ROS) production, and may contribute to pattern separation. In Experiment 1, we performed RNA sequencing of the dorsal hippocampus 16 days after stress in rats that either develop conditioned place avoidance to a predator urine-associated …

2023 February 17 - Board Of Trustees Agenda And Minutes, Board Of Trustees, East Tennessee State University

Board of Trustees Agendas and Minutes

No abstract provided.

2023 February 17 - Board Of Trustees Academic, Research, And Student Success Committee Agenda And Minutes, Board Of Trustees, East Tennessee State University

Board of Trustees Agendas and Minutes

No abstract provided.

Cd5 Expression By Dendritic Cells Directs T Cell Immunity And Sustains Immunotherapy Responses, Mingyu He, Kate Roussak, Feiyang Ma, Nicholas Borcherding, Vince Garin, Mike White, Charles Schutt, Trine I Jensen, Yun Zhao, Courtney A Iberg, Kairav Shah, Himanshi Bhatia, Daniel Korenfeld, Sabrina Dinkel, Judah Gray, Alina Ulezko Antonova, Stephen Ferris, David Donermeyer, Cecilia Lindestam Arlehamn, Matthew M Gubin, Jingqin Luo, Laurent Gorvel, Matteo Pellegrini, Alessandro Sette, Thomas Tung, Rasmus Bak, Robert L Modlin, Ryan C Fields, Robert D Schreiber, Paul M Allen, Eynav Klechevsky

Faculty, Staff and Student Publications

The induction of proinflammatory T cells by dendritic cell (DC) subtypes is critical for antitumor responses and effective immune checkpoint blockade (ICB) therapy. Here, we show that human CD1c+CD5+ DCs are reduced in melanoma-affected lymph nodes, with CD5 expression on DCs correlating with patient survival. Activating CD5 on DCs enhanced T cell priming and improved survival after ICB therapy. CD5+ DC numbers increased during ICB therapy, and low interleukin-6 (IL-6) concentrations promoted their de novo differentiation. Mechanistically, CD5 expression by DCs was required to generate optimally protective CD5hi T helper and CD8+ T cells; further, deletion of CD5 from T …

Antiangiogenic Drugs In Combination With Seaweed Fucoidan: A Mechanistic In Vitro And In Vivo Study Exploring The Vegf Receptor And Its Downstream Signaling Molecules In Hepatic Cancer, Maha R.A. Abdollah, Aya A. Ali, Hassnaa Hamed Elgohary, M. M. Elmazar

Pharmacy

Hepatocellular carcinoma (HCC) is one of the most common cancers reported worldwide with poor morbidity and high mortality rates. HCC is a very vascular solid tumour as angiogenesis is not only a key driver for tumour progression but also an exciting therapeutic target. Our research investigated the use of fucoidan, a sulfated polysaccharide readily abundant in edible seaweeds commonly consumed in Asian diet due to their extensive health benefits. Fucoidan was reported to possess a strong anti-cancer activity, but its anti-angiogenic potential is still to be fully unraveled. Our research investigated fucoidan in combination with sorafenib (an anti-VEGFR tyrosine kinase …

Fort Hays State University Tiger Weekly, February 17, 2023, University Relations

Tiger Weekly Newsletters

No abstract provided.

Developing Methods For Measuring In Vivo Lipid Metabolism, Russell L. Denton

Theses and Dissertations

Lipid metabolism is critically important to the normal function of individual cells and for signaling between tissues of the human body. There is a great need to quantify changes in lipid metabolism because of its implications in both normal healthy conditions and during disease development. In our first study, we developed a liquid chromatography mass spectrometry based workflow to assess in vivo metabolism of murine brain lipids. This involved sample preparation, data acquisition, and analysis software development and improvements. Regarding sample preparation, we maintained the mice at 5% body water for the duration of the experiment. As mouse metabolism proceeds, …

An Innovative Approach To Improve Oil Production And Quality Of Mustard (Brassica Juncea L.) With Multi-Nutrient-Rich Polyhalite, Biswajit Pramanick, B.S. Mahapatra, Debarati Datta, Prithwiraj Dey, S.P. Singh, Ajay Kumar, Bappa Paramanik, Neeraj Awasthi

Department of Agronomy and Horticulture: Faculty Publications

Polyhalite popularly known as POLY4 is a multi-nutrient fertiliser containing K, S, Mg, Ca, and micronutrients. POLY4 has a low carbon footprint, is certified for organic agriculture, and has the potential to improve crop productivity and quality attributes Indian mustard which often faces challenges due to imbalanced nutrition supplied in the current fertilisation schedule. The hypothesis of the study was that the multi-nutrient fartiliser POLY4 can ensure balanced nutrition for Indian mustard. Considering this, a field experiment was conducted during the winter seasons of 2017–18 and 2018–19 to evaluate the effect of POLY4 on Indian mustard (Brassica juncea L.) …

Sakurabonsai: Protocol Design Of A Novel, Prospective Study To Explore Clinical, Imaging, And Biomarker Outcomes In Patients With Aqp4-Igg-Seropositive Neuromyelitis Optica Spectrum Disorder Receiving Open-Label Satralizumab, Jeffrey L Bennett, Kazuo Fujihara, Ho Jin Kim, Romain Marignier, Kevin C O'Connor, Robert C Sergott, Anthony Traboulsee, Heinz Wiendl, Jens Wuerfel, Scott S Zamvil, Veronica G Anania, Regine Buffels, Thomas Künzel, Annemarie N Lekkerkerker, Siân Lennon-Chrimes, Sean J Pittock

Wills Eye Hospital Papers

Background: Neuromyelitis optica spectrum disorder (NMOSD) is a rare, autoimmune disease of the central nervous system that produces acute, unpredictable relapses causing cumulative neurological disability. Satralizumab, a humanized, monoclonal recycling antibody that targets the interleukin-6 receptor, reduced NMOSD relapse risk vs. placebo in two Phase 3 trials: SAkuraSky (satralizumab ± immunosuppressive therapy; NCT02028884) and SAkuraStar (satralizumab monotherapy; NCT02073279). Satralizumab is approved to treat aquaporin-4 IgG-seropositive (AQP4-IgG+) NMOSD. SAkuraBONSAI (NCT05269667) will explore fluid and imaging biomarkers to better understand the mechanism of action of satralizumab and the neuronal and immunological changes following treatment in AQP4-IgG+ NMOSD.

Objectives: …

Tracking The Evolution Of Esophageal Squamous Cell Carcinoma Under Dynamic Immune Selection By Multi-Omics Sequencing, Sijia Cui, Nicholas Mcgranahan, Jing Gao, Peng Chen, Wei Jiang, Lingrong Yang, Li Ma, Junfang Liao, Tian Xie, Congying Xie, Tariq Enver, Shixiu Wu

Children’s Nutrition Research Center Staff Publications

Intratumoral heterogeneity (ITH) has been linked to decreased efficacy of clinical treatments. However, although genomic ITH has been characterized in genetic, transcriptomic and epigenetic alterations are hallmarks of esophageal squamous cell carcinoma (ESCC), the extent to which these are heterogeneous in ESCC has not been explored in a unified framework. Further, the extent to which tumor-infiltrated T lymphocytes are directed against cancer cells, but how the immune infiltration acts as a selective force to shape the clonal evolution of ESCC is unclear. In this study, we perform multi-omic sequencing on 186 samples from 36 primary ESCC patients. Through multi-omics analyses, …

A Review And Comparison Of The Film And Series Versions Of "A League Of Their Own", Emily Stier Adler, Roger Clark

Sociology Between the Gaps: Forgotten and Neglected Topics

No abstract provided.

Predicting Heart Failure Readmission Using Home Health Clinical Indices, Jamie Murphy

Dissertations

Heart failure is the most rapidly growing cardiovascular disease in the U. S. (Boxer et al., 2010) and is the most common cause for hospital readmission (Fang et al., 2008; Heidenreich et al., 2013; Jencks et al., 2009) with a 30-day readmission rate of 24.8% (Centers for Medicare and Medicaid, 2012). Currently, many heart failure patients are discharged quickly from the hospital without adequate skills and knowledge to perform self-care tasks. Moreover, home health nurses provide the same care for all heart failure patients and have limited knowledge on what clinical health indicators might affect readmission. As a result, home …

The Effects Of Simulation On Nursing Student Knowledge, Attitudes, And Clinical Preparedness In The Care Of Transgender Clients, Julie Cartney

Dissertations

Nationally, nursing education provides extremely limited coverage of LGBTQIA+ topics and as a result, nursing students lack clinical confidence and knowledge to assess and intervene effectively in this marginalized population. Despite national health goals and organizational calls for health equity, transgender persons continue to experience significant healthcare disparities and barriers in accessing quality health care.

The purpose of this project was to determine if the incorporation of a simulation scenario that features the care of transgender patient would have a positive effect on the knowledge, attitudes, and clinical preparedness of practical nursing students.

Exploiting Metabolic Vulnerabilities After Anti-Vegf Antibody Therapy In Ovarian Cancer, Deanna Glassman, Mark S Kim, Meredith Spradlin, Sunil Badal, Mana Taki, Pratip Bhattacharya, Prasanta Dutta, Charles V Kingsley, Katherine I Foster, Olamide Animasahun, Jin Heon Jeon, Abhinav Achreja, Anusha Jayaraman, Praveen Kumar, Minal Nenwani, Fulei Wuchu, Emine Bayraktar, Yutuan Wu, Elaine Stur, Lingegowda Mangala, Sanghoon Lee, Timothy A Yap, Shannon N Westin, Livia S Eberlin, Deepak Nagrath, Anil K Sood

Faculty, Staff and Student Publications

Despite modest clinical improvement with anti-vascular endothelial growth factor antibody (AVA) therapy in ovarian cancer, adaptive resistance is ubiquitous and additional options are limited. A dependence on glutamine metabolism, via the enzyme glutaminase (GLS), is a known mechanism of adaptive resistance and we aimed to investigate the utility of a GLS inhibitor (GLSi). Our in vitro findings demonstrated increased glutamine abundance and a significant cytotoxic effect in AVA-resistant tumors when GLSi was administered in combination with bevacizumab. In vivo, GLSi led to a reduction in tumor growth as monotherapy and when combined with AVA. Furthermore, GLSi initiated after the emergence …

Corrigendum: Strengthened Multi-Stakeholder Linkages In Valuation Studies Is Critical For Improved Decision Making Outcomes For Valuable Mangroves – The Malaysian Case Study, Soon Loong Lee, Amy Yee Hui Then, Hong Ching Goh, Caroline Hattam, Andrew Edwards-Jones, Melanie C. Austen

School of Biological and Marine Sciences

No abstract provided.

Exploiting Prmt5 As A Target For Combination Therapy In Mantle Cell Lymphoma Characterized By Frequent Atm And Tp53 Mutations, Yuxuan Che, Yang Liu, Yixin Yao, Holly A Hill, Yijing Li, Qingsong Cai, Fangfang Yan, Preetesh Jain, Wei Wang, Lixin Rui, Michael Wang

Faculty, Staff and Student Publications

Constant challenges for the treatment of mantle cell lymphoma (MCL) remain to be recurrent relapses and therapy resistance, especially in patients harboring somatic mutations in the tumor suppressors ATM and TP53, which are accumulated as therapy resistance emerges and the disease progresses, consistent with our OncoPrint results that ATM and TP53 alterations were most frequent in relapsed/refractory (R/R) MCL. We demonstrated that protein arginine methyltransferase-5 (PRMT5) was upregulated in R/R MCL, which predicted a poor prognosis. PRMT5 inhibitors displayed profound antitumor effects in the mouse models of MCL with mutated ATM and/or TP53, or refractory to CD19-targeted CAR T-cell therapy. …