Digital Commons Network^™

Repurposing Atovaquone As A Therapeutic Against Acute Myeloid Leukemia (Aml): Combination With Conventional Chemotherapy Is Feasible And Well Tolerated, Alexandra Mclean Stevens, Eric S Schafer, Minhua Li, Maci Terrell, Raushan Rashid, Hana Paek, Melanie B Bernhardt, Allison Weisnicht, Wesley T Smith, Noah J Keogh, Michelle C Alozie, Hailey H Oviedo, Alan K Gonzalez, Tamilini Ilangovan, Alicia Mangubat-Medina, Haopei Wang, Eunji Jo, Cara A Rabik, Claire Bocchini, Susan Hilsenbeck, Zachary T Ball, Todd M Cooper, Michele S Redell

Center for Medical Ethics and Health Policy Staff Publications

Survival of pediatric AML remains poor despite maximized myelosuppressive therapy. The pneumocystis jiroveci pneumonia (PJP)-treating medication atovaquone (AQ) suppresses oxidative phosphorylation (OXPHOS) and reduces AML burden in patient-derived xenograft (PDX) mouse models, making it an ideal concomitant AML therapy. Poor palatability and limited product formulations have historically limited routine use of AQ in pediatric AML patients. Patients with de novo AML were enrolled at two hospitals. Daily AQ at established PJP dosing was combined with standard AML therapy, based on the Medical Research Council backbone. AQ compliance, adverse events (AEs), ease of administration score (scale: 1 (very difficult)-5 (very easy)) …

Comparison Of Two Diagnostic Assays For The Detection Of Serum Neutralizing Antibody To Porcine Epidemic Diarrhea Virus, Justin Brown, Korakrit Poonsuk, Ting-Yu Cheng, Chris Rademacher, Erin Kalkwarf, Liying Tian, Lauren A. Mckeen, Chong Wang, Luis Gimenez-Lirola, Gimenez-Lirola Baum, Locke A. Karriker

Nebraska Center for Virology: Faculty Publications

Lactogenic immunity is important for the protection of piglets against many pathogens including porcine epidemic diarrhea virus. Circulating neutralizing antibodies levels in sow sera may help determine if a detectable immune response could confer protection to piglets. Neutralizing antibodies can be detected through various diagnostic assays. This study evaluated the diagnostic characteristics of two neutralizing antibody assays for porcine epidemic diarrhea virus neutralizing antibodies in serum of challenged gilts. Four treatment groups, control, non-vaccinated, vaccinated prior to challenge, and vaccinated following challenge, were comprised of 20 gilts. Serum sample were collected from each gilt prior to and following challenge with …

Combining Corn N Recommendation Tools For An Improved Economical Optimal Nitrogen Rate Estimation, Curtis J. Ransom, Newell R. Kitchen, James J. Camberato, Paul R. Carter, Richard B. Ferguson, Fabián G. Fernández, David W. Franzen, Carrie A. M. Laboski, David Brenton Myers, Emerson D. Nafziger, John E. Sawyer, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Improving corn (Zea mays L.) nitrogen (N) rate fertilizer recommendation tools can improve farmers’ profits and mitigate N pollution. Numerous approaches have been tested to improve these tools, but to date improvements for predicting economically optimum N rate (EONR) have been modest. This work’s objective was to use ensemble learning to improve our estimation of EONR (for a single at-planting and split N application timing) by combining multiple corn N recommendation tools. The evaluation was conducted using 49 corn N response trials from eight states in the US Corn Belt and three growing seasons (2014–2016). Elastic net and decision …

Plant Species’ Capacity For Range Shifts At The Habitat And Geographic Scales: A Trade-Off-Based Framework, Bailey H. Mcnichol, Sabrina E. Russo

School of Biological Sciences: Faculty Publications

Climate change is causing rapid shifts in the abiotic and biotic environmental conditions experienced by plant populations, but we lack generalizable frameworks for predicting the consequences for species. These changes may cause individuals to become poorly matched to their environments, potentially inducing shifts in the distributions of populations and altering species’ habitat and geographic ranges. We present a trade-off-based framework for understanding and predicting whether plant species may undergo range shifts, based on ecological strategies defined by functional trait variation. We define a species’ capacity for undergoing range shifts as the product of its colonization ability and the ability to …

Dna Methylation And Histone Modification In Low-Grade Gliomas: Current Understanding And Potential Clinical Targets, Ahmad Ozair, Atulya Khosla, Rupesh Kotecha, Yazmin Odia, Michael Mcdermott, Manmeet Ahluwalia

All Publications

Gliomas, the most common type of malignant primary brain tumor, were conventionally classified through WHO Grades I–IV (now 1–4), with low-grade gliomas being entities belonging to Grades 1 or 2. While the focus of the WHO Classification for Central Nervous System (CNS) tumors had historically been on histopathological attributes, the recently released fifth edition of the classification (WHO CNS5) characterizes brain tumors, including gliomas, using an integration of histological and molecular features, including their epigenetic changes such as histone methylation, DNA methylation, and histone acetylation, which are increasingly being used for the classification of low-grade gliomas. This review describes the …

Tcr-Engineered Adoptive Cell Therapy Effectively Treats Intracranial Murine Glioblastoma, Maximilian O. Schaettler, Rupen Desai, Anthony Z. Wang, Alexandra J. Livingstone, Dale K. Kobayashi, Andrew T. Coxon, Jay A. Bowman-Kirigin, Connor J. Liu, Mao Li, Diane E. Bender, Michael J. White, David M. Kranz, Tanner M. Johanns, Gavin P. Dunn

2020-Current year OA Pubs

BACKGROUND: Adoptive cellular therapies with chimeric antigen receptor T cells have revolutionized the treatment of some malignancies but have shown limited efficacy in solid tumors such as glioblastoma and face a scarcity of safe therapeutic targets. As an alternative, T cell receptor (TCR)-engineered cellular therapy against tumor-specific neoantigens has generated significant excitement, but there exist no preclinical systems to rigorously model this approach in glioblastoma.

METHODS: We employed single-cell PCR to isolate a TCR specific for the Imp3

RESULTS: We isolated and characterized the 3×1.1C TCR that displayed a high affinity for mImp3 but no wild-type cross-reactivity. To provide a …

Hydrogel Encapsulation Of Genome-Engineered Stem Cells For Long-Term Self-Regulating Anti-Cytokine Therapy, Kelsey H Collins, Lara Pferdehirt, Leila S Saleh, Alireza Savadipour, Luke E Springer, Kristin L Lenz, Dominic M Thompson, Sara J Oswald, Christine T N Pham, Farshid Guilak

2020-Current year OA Pubs

Biologic therapies have revolutionized treatment options for rheumatoid arthritis (RA) but their continuous administration at high doses may lead to adverse events. Thus, the development of improved drug delivery systems that can sense and respond commensurately to disease flares represents an unmet medical need. Toward this end, we generated induced pluripotent stem cells (iPSCs) that express interleukin-1 receptor antagonist (IL-1Ra, an inhibitor of IL-1) in a feedback-controlled manner driven by the macrophage chemoattractant protein-1 (Ccl2) promoter. Cells were seeded in agarose hydrogel constructs made from 3D printed molds that can be injected subcutaneously via a blunt needle, thus simplifying implantation …

Decision-Making Under Risk And Theory Of Mind In Adolescent Offenders In Provisional Deprivation Of Liberty, Rubens José Loureiro, Flavio Takemi Kataoka, Thiago Wendt Viola, Gisele Iesbich Vargas, Breno Sanvicente-Vieira, Rodrigo Grassi-Oliveira, Bruno Kluwe-Schiavon

Faculty, Staff and Student Publications

INTRODUCTION: Delinquent behaviors are risky behaviors that increase during puberty and reach their highest peak in late adolescence. It has been proposed that poor decision-making and theory of mind (ToM) are key cognitive processes implicated with delinquency during adolescence, affecting evaluation of risks and impairing appreciation of social norms. Nevertheless, it is not yet clear whether adolescent offenders who are subjected to provisional deprivation of liberty due to conflict with the law (adolescents in conflict with the law [ACL]) might, in fact, present a specific profile with regard to these cognitive processes.

OBJECTIVES: To assess deliberative decision-making and ToM among …

Development Of A Slow-Degrading Polymerized Curcumin Coating For Intracortical Microelectrodes, Alexis M. Ziemba, Mary Clare Crochiere Woodson, Jessica L. Funnell, Douglas Wich, Bailey Balouch, Deniz Rende, Dahlia N. Amato, Jonathan Bao, Ingrid Oprea, Dominica Cao, Neda Bajalo, Evon S. Ereifej, Jeffrey R. Capadona, Edmund F. Palermo, Ryan J. Gilbert

Chemical and Biochemical Engineering Faculty Research & Creative Works

Intracortical microelectrodes are used with brain-computer interfaces to restore lost limb function following nervous system injury. While promising, recording ability of intracortical microelectrodes diminishes over time due, in part, to neuroinflammation. As curcumin has demonstrated neuroprotection through anti-inflammatory activity, we fabricated a 300 nm-thick intracortical microelectrode coating consisting of a polyurethane copolymer of curcumin and polyethylene glycol (PEG), denoted as poly (curcumin-PEG1000 carbamate) (PCPC). The uniform PCPC coating reduced silicon wafer hardness by two orders of magnitude and readily absorbed water within minutes, demonstrating that the coating is soft and hydrophilic in nature. Using an in vitro release model, curcumin …

Dosimetric Delivery Validation Of Dynamically Collimated Pencil Beam Scanning Proton Therapy, Jen Yu, Alonso Gutierrez

All Publications

Objective. Pencil beam scanning (PBS) proton therapy target dose conformity can be improved with energy layer-specific collimation. One such collimator is the dynamic collimation system (DCS), which consists of four nickel trimmer blades that intercept the scanning beam as it approaches the lateral extent of the target. While the dosimetric benefits of the DCS have been demonstrated through computational treatment planning studies, there has yet to be experimental verification of these benefits for composite multi-energy layer fields. The objective of this work is to dosimetrically characterize and experimentally validate the delivery of dynamically collimated proton therapy with the DCS …

Chimes: February 20, 2023, Calvin University

Chimes

Rangeela returns by Savannah Shustack

Calvin community responds to shooting at Michigan State University by Abigail Ham

As AI takes its next big step, schools ponder how to respond by David Ogboro

Communication issues and lack of clarity have faculty questioning leadership team's role by Grace Buller

Former Dance Guild choreographers spearhead new Calvin Knights Cheer Program by Lauren Nyong

What goes up(stream) must come down(stream) by Lainey Moore

From "Institutes" to Confessions: anonymous confessions at Calvin by Katie Rosendale

Differential Regulation Of H3k9/H3k14 Acetylation By Small Molecules Drives Neuron-Fate-Induction Of Glioma Cell, Xincheng Liu, Cui Guo, Tiandong Leng, Zhen Fan, Jialuo Mai, Jiehong Chen, Jinhai Xu, Qianyi Li, Bin Jiang, Ke Sai, Wenzhuo Yang, Jiayu Gu, Jingyi Wang, Shuxin Sun, Zhijie Chen, Yingqian Zhong, Xuanming Liang, Chaoxin Chen, Jing Cai, Yuan Lin, Jiankai Liang, Jun Hu, Guangmei Yan, Wenbo Zhu, Wei Yin

Faculty, Staff and Student Publications

Differentiation therapy using small molecules is a promising strategy for improving the prognosis of glioblastoma (GBM). Histone acetylation plays an important role in cell fate determination. Nevertheless, whether histone acetylation in specific sites determines GBM cells fate remains to be explored. Through screening from a 349 small molecule-library, we identified that histone deacetylase inhibitor (HDACi) MS-275 synergized with 8-CPT-cAMP was able to transdifferentiate U87MG GBM cells into neuron-like cells, which were characterized by cell cycle arrest, rich neuron biomarkers, and typical neuron electrophysiology. Intriguingly, acetylation tags of histone 3 at lysine 9 (H3K9ac) were decreased in the promoter of multiple …

Identification Of And Mechanistic Insights Into Sars-Cov-2 Main Protease Non-Covalent Inhibitors: An In-Silico Study, Jian-Xin Shen, Wen-Wen Du, Yuan-Ling Xia, Zhi-Bi Zhang, Ze-Fen Yu, Yun-Xin Fu, Shu-Qun Liu

Faculty, Staff and Student Publications

The indispensable role of the SARS-CoV-2 main protease (Mpro) in the viral replication cycle and its dissimilarity to human proteases make Mpro a promising drug target. In order to identify the non-covalent Mpro inhibitors, we performed a comprehensive study using a combined computational strategy. We first screened the ZINC purchasable compound database using the pharmacophore model generated from the reference crystal structure of Mpro complexed with the inhibitor ML188. The hit compounds were then filtered by molecular docking and predicted parameters of drug-likeness and pharmacokinetics. The final molecular dynamics (MD) simulations identified three effective candidate inhibitors (ECIs) capable of maintaining …

Multiple Fibrofolliculomas Within A Fibrous Cephalic Plaque In A Patient With Tuberous Sclerosis, Justin Raman, Annie Jin, Jennifer J. Parker, Conor M. Vickers, Daniela M. Proca, Sylvia Hsu, Jason B. Lee

Department of Dermatology and Cutaneous Biology Faculty Papers

No abstract provided.

De Novo Assembly And Comparative Analyses Of Mitochondrial Genomes In Piperales, Runxian Yu, Xudong Chen, Lingjie Long, Matthias Jost, Ran Zhao, Lumei Liu, Jeffrey P. Mower, Claude W. Depamphilis, Stefan Wanke, Yuannian Jiao

Department of Agronomy and Horticulture: Faculty Publications

The mitochondrial genome of Liriodendron tulipifera exhibits many ancestral angiosperm features and a remarkably slow evolutionary rate, while mitochondrial genomes of other magnoliids remain yet to be characterized. We assembled nine new mitochondrial genomes, representing all genera of perianth-bearing Piperales, as well as for a member of the sister clade: three complete or nearly complete mitochondrial genomes from Aristolochiaceae and six additional draft assemblies including Thottea, Asaraceae, Lactoridaceae, and Hydnoraceae. For comparative purpose, a complete mitochondrial genome was assembled for Saururus, a member of the perianth-less Piperales. The average number of short repeats (50–99 bp) was much larger …

Shared Responsibility: Conceptualising How A Public Health Approach May Enhance Police Response To Missing Persons, Katie Gambier-Ross, Joe Apps Dr, Sarah Wayland Dr

International Journal of Missing Persons

When a person is reported missing there are substantial costs for the individual, their family and society. This paper conceptualises the experience of missing persons episodes, through a public health approach. This then allows police, stakeholders and the community to engage in discussions about who is vulnerable to going missing by intervening in a way that addresses risk. Historically, a missing persons episode involves an absence, typically followed by police involvement in consultation with next of kin with establishing the whereabouts of the missing person being the primary focus. Yet, the risk factors of going missing relate more to the …

Research Progress Of Change Rules In Freezing Denaturation Of Surimi Protein And Its Regulatory Methods, Yu Lu-Han, Chen Xu, Cai Xi-Xi, Du Ming, Wu Jin-Hong, Huang Jian-Lian, Wang Shao-Yun

Food and Machinery

The change rule of main indexes of frozen surimi was introduced, and the influencing factors of surimi protein denaturation were briefly analyzed and discussed. Moreover, the regulation methods of surimi protein in antifreeze were reviewed.

Effect Of Ultrasonic Pretreatment On Water Migration Of Apricot Slices During Microwave Freeze-Drying, Jin Li-Wei, Ren Guang-Yue, Duan Xu, Zhang Ying-Min, Qu Zhan-Ping, Li Xin-Lin

Food and Machinery

To explore the drying characteristics and moisture migration and diffusion characteristics of apricot slices in the microwave freeze-drying process of ultrasonic pretreatment, microwave vacuum-freeze drying technology was selected, and the transverse relaxation time T2 inversion spectra of apricot slices during MFD drying under different ultrasonic pretreatment conditions were measured by low field nuclear magnetic resonance. The results showed that ultrasonic pretreatment had a great influence on the drying rate of apricot slices in the early stage of MFD. Scanning electron microscopy showed that the best ultrasonic treatment condition for internal structure was 350 W, 35 ℃, 15 min, and it …

Study On The Hypolipidemic Function Of Noni Wine, Li Xiao-Juan

Food and Machinery

Established a mouse model of hyperlipidemia. The male mice were divided into blank control group, low fat group and high fat group, and the low dose, medium dose, high dose of Noni wine. Serum concentrations of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), respiratory exchange ratio (RER), were measured after 90 days of feeding. The results showed that medium dose and high dose can effectively reduce the body weight or body weight gain of the obese mice, and the testis fat rate, renal fat rate or body fat rate decreased. The serum TC content, …

Development And Quality Evaluation Of Compound Rice Noodles Based On Traditional Recipe For Strengthening Spleen Diet, Zhang Jin-Yao, Tan Wen-Gen, Fan Yu-Bing, Zheng Tao, Zeng Yi-Qiong, Zheng Hui, Yang Yong

Food and Machinery

In order to improve the nutrition and health quality of traditional rice noodles, a special compound rice noodles was developed, using traditional rice noodles technology based on traditional recipe for strengthening spleen diet "Yang chun bai xue cake". In this study, the influence of the amount of single accessories for the recipe (poria cocos, yam, lotus seed, euryales semen) on the quality of rice noodles was used to determine the composition ratio of compound accessories. The amount of compound accessories, xanthan gum and water in the compound rice noodle formula was optimized, and the texture characteristics, product aging degree and …

Optimizing Conditions Of Producing Bacteriocin By Lactobacillus Plantarum M1-Ntg300, An Yu, Wang Ying, Yi Hua-Xi, Zhang Dong-Jie

Food and Machinery

Taking the potency of phytolactin as a characteristic index, the culture conditions for bacteriocin production by Lactobacillus plantarum M1-NTG300 were optimized. Through response surface analysis combined with actual values, the optimal components of the culture medium were determined to include 3.5% glucose concentration, 3.5% peptone, 0.35% Tween 80, and 0.20% magnesium chloride. Under the control of these culture conditions, The titer of bacteriocin, produced by L. plantarum M1-NTG300 was 1 269.4 IU/mL, which was increased by 19.8%.

Effect Of Salt Amount On The Quality Of Chimonobambusa Quadrangularis Shoots Dried By Salt Boiling, Lv Chao-Yan, Gao Zhi-Xi, Liu De-Fen, Jiang Qi, Wang Mi, Ma Yun-Fei, Cai Chun

Food and Machinery

Different amounts of salt (10%, 15%, 20%, 25%, and 30%) were set to cook Chimonobambusa quadrangularis shoots. The effects of salt amount on total sugar, reducing sugar, soluble protein, free amino acid, NaCl, nitrite, as well as moisture content, rehydration ratio, color, sensory score, were studied. The results showed that the water content decreases with the increase of boiling time, showing the salt amount dependent. The rehydration rate increased with the rehydration time, and when it basically stops increasing, the rehydration ratio was 10%＞15%＞20%＞30%＞25% in descending order. The total sugar, reducing sugar, soluble protein, and free amino acid content all …

Effects Expansion Process On Tobacco Quality, Chong Li-Min, Lu Yuan, Jiang Guang

Food and Machinery

To analyze the chemical composition and aroma composition of tobacco before and after expansion in order to develop a carbon dioxide expansion process for producing high-quality tobacco. The influence of different carbon expansion technology conditions on the physical processing quality and sensory quality of expanded tobacco was determined and the trend of change was determined by the single factor test method. After different temperature of carbon dioxide expansion, HT treatment and drying, the variation trend of tobacco aroma composition and sensory quality were basically the same, but the variation trend of tobacco aroma composition and sensory quality decreased more obviously …

Recent Advances In Microbial Pectinase, Yang Tong-Xiang, Wu Kong-Yang, Bai Yun-Fei, Chen Jun-Liang, Wan Su-Yan, Yang Ru-Meng

Food and Machinery

This review covered the advances in the screening of microbial pectinase, breeding technology, some factors affecting pectinase production, enzymatic properties, immobilization, and the development prospects of microbial pectinase were outlined.

Progress In Preparation And Application Of Double Emulsion, Wen-Hua Xu, Jia Zhang, Zi-Han Wang, Zhuo Jiang, Sheng-Quan Huan, Xian Wu

Food and Machinery

In this study, the latest research results of droplet microfluidics, membrane emulsification, and coaxial electrospray preparation of double emulsion are introduced, and their advantages and disadvantages are compared and analyzed. The limitation of microfluidic prepared double emulsion industrialization and its related research was pointed out. The latest research results of double emulsion in the fields of food, cosmetics, and biomedicine were reviewed, and the advantages and prospects of double emulsion applications prospected.

Differential Proteomics Analysis Of Egg Whites At Different Spray Drying Inlet Temperatures, Li-Li Liu, Sheng-Juan Shi, Ke Chen, Meng-Jun Zhang, Wei-Ming Hao, Yuan-Yuan Li

Food and Machinery

The purpose of this study was to investigate the protein expression of egg white protein at different inlet temperatures of spray drying (140,160,180 ℃). ITRAQ technology was used to identify and bioinformatics egg white proteins, and to investigate the distribution, function and mechanism of different proteins in egg white powder during different drying processes. The results showed that, compared with the control group at 140 ℃, 41 and 46 different proteins were identified at 160 ℃ and 180 ℃ respectively during the spray drying process of egg white (Fold change ＞1.2, P＜0.05). GO function annotation analyzed the biological processes that …

Simultaneous Determination Of 8 Adipate Plasticizers In Infant Formula By Isotope Dilution-Gas Chromatography-Mass Spectrometry, Yong-Hui Huang

Food and Machinery

A method was developed for the simultaneous determination of 8 adipate plasticizers in infant formula by isotope dilution-gas chromatography-mass spectrometry (GC-MS). With the infant formula sample completely dissolved in water, n-hexane was used as the extraction solvent. After ultrasonic extraction, qualitative and quantitative analysis were carried out by GC-MS in selected ion monitoring modes. The correlation coefficients (R²) of 8 adipate plasticizers were over 0.999, The limits of detection (LODs) were 0.08~0.12 mg/kg and the limits of quantification(LODs) were 0.2~0.4 mg/kg. The average recoveries of 8 adipate plasticizers in milk powder were ranged from 94.8% to 103.3%. In …

Effects Of Different Organic Acid Impregnating Treatments On The Quality Of Freeze-Dried Apple Slices, Qian-Ju Wang, Ting-Cai Yan, Qiu-Ju Yan, Chun Hua, Feng Zhou, Hai-Ou Wang

Food and Machinery

The fresh-cut apple slices were impregnated with different concentrations (1%, 2%, 3% and 4%) of organic acid including citric acid, ascorbic acid, malic acid and acetic acid. The color, yield, rehydration, deformation rate, texture characteris-tics and microscopic pore structure of freeze-dried apple chips were used as indicators to analyze the effect of different acid immersion on the quality of vacuum freeze-dried apple chips. The results showed that the △E value of freeze-dried apple slices treated with citric acid, ascorbic acid and malic acid increased with the increase of acid concentration, with relatively small △E value treated with acetic acid, …

Optimization Of Preparation Technology And Structural Characterization Of Tarragon Essential Oil Nanocapsules, Ying Liang, Hui-Yun Zhang, Huai-Bin Kang

Food and Machinery

Chitosan-tarragon essential oil nanocapsules were prepared by ion gelation method. The effects of the mass ratios of tarragon essential oil to chitosan, pH of chitosan solution, and the mass ratio of sodium tripolyphosphate to chitosan on the encapsulation rate of tarragon essential oil nanocapsules were investigated. Box-Behnken experiment design and response surface analysis were used to optimize the preparation process of nanocapsules, and the quadratic regression model was established. The results showed that the optimum preparation process of tarragon essential oil nanocapsules was the mass ratio of tarragon essential oil to chitosan 2∶1, pH=3.5, and the mass ratio of sodium …

Research Progress On The Extraction Of Plant Polyphenols, Fu-Lin Tian, Wen-Jing Huang, Zhan Wang, Xian-Kui Qin, Wang-Yang Shen, Qiao-Yu Cai

Food and Machinery

In this review, the non-mentioned extraction methods of plant polyphenol in the existing research are summarized, including subcritical extraction, low eutectic extraction, and pressurized extraction, etc. The principles, advantages and disadvantages of different extraction methods are deeply analyzed. Moreover, the development direction of plant polyphenol extraction is prospected.