Digital Commons Network^™

The “Best Test” For Success In Academic Advising: Exploring The Purpose Of Advising Through The Lens Of Servant-Leadership., Jeffrey Mcclellan

Servant Leadership: Theory & Practice

For years, advising has been associated with student success and retention. However, while these are valuable byproducts of effective academic advising, they do not represent the purpose of advising. This article explores the purpose of academic advising through the lens of servant-leadership theory with an emphasis on Greenleaf’s (2002) best test, which asks, “Do those served grow as persons? Do they, while being served, become healthier, wiser, freer, more autonomous, more likely themselves to become servants?” (p. 27). The outcomes alluded to in this statement are examined and explored as they relate to the purpose of academic advising and student …

Analysis Of Biologically Active Secondary Metabolites From Fungi, Elizabeth E. Guevara, Kirk Manfredi

Summer Undergraduate Research Program (SURP) Symposium

Fungi produce secondary metabolites, which are organic compounds that allow the organism to be competitive in their environment. Secondary metabolites are not involved in growth and development, instead they affect ecological interactions to increase survivability. These compounds will often have antimicrobial properties, leading to their use in antibiotics. Isolating a compound with significant antimicrobial activity could lead to its use in therapeutic antibiotics. New antibiotics are needed to address the growing issue of antimicrobial resistant bacteria. This project explores the antimicrobial compounds produced by fungi collected Wind Cave National Park or carried by local insects. Accurate taxonomic identification of the …

Functional Analysis Of Spermidine Synthase (Spds) Using Virus-Induced Gene Silencing In Barley, Ammar A. Shaikh, Tilahun Abebe

Summer Undergraduate Research Program (SURP) Symposium

Polyamines are low molecular weight aliphatic nitrogenous compounds with two or more amino groups (Liu et al., 2015). Polyamines are found in all organisms. They play diverse roles in growth and development as well as environmental stress response. One of the major polyamines in plants is spermidine. Spermidine increases grain filling by increasing hormones and enzymes in sucrose biosynthesis (Luo et al., 2019). Expression of genes in the spermidine biosynthesis pathway increases during drought in barley (Abebe et al., 2010). However, the role of spermidine biosynthesis for drought tolerance has not been systematically investigated in barley. In the past, we …

Analysis Of The Role Of Gamma-Aminobutyrate (Gaba) In Drought Tolerance In Barley Using Virus-Induced Gene Silencing, Thomas Manternach, Tilahun Abebe

Summer Undergraduate Research Program (SURP) Symposium

Gamma-aminobutyric acid (GABA) is a messenger in cellular communication. As GABA is also an enhancer in stress tolerance, this often leads to rapid accumulation during drought. . GABA increases photosynthesis and reduces water loss through the stomata (Li et al., 2021; Xu et al., 2021). The metabolization of GABA occurs via the GABA shunt, which is produced primarily from a glutamic acid catalyzed by glutamate decarboxylase (GAD) in the cytosol. GABA is imported into the mitochondria and subsequently degraded by GABA-transaminase (GABA-T) into succinic semialdehyde (SSA) . In barley, both GABA and GAD genes are induced during drought (Abebe et …

Comparison Of Gut And Leg Pollen Composition In Female Halictus Ligatus With Flower Source, Sriya Kalala, Ai Wen

Summer Undergraduate Research Program (SURP) Symposium

It is commonly believed by bee biologists that wild bees visit one single flower species during each trip to make nest provision. However, the exact foraging behavior is not clear and may vary in different species of bees, and therefore the pollination patterns have continued to be studied. When a bee undergoes the process of pollination, the majority of the pollen consumed by the bee passes through the digestive tract and remains undigested. However, when the pollen is digested and used as an energy source, the pollen grain digestion occurs in the midgut intestine within the bee instead of the …

Iowa’S Agricultural Landscape: Is Tef A Viable Option?, Kyran Hines, Tilahun Abebe

Summer Undergraduate Research Program (SURP) Symposium

Tef (Eragrostis tef Zucc.) Trotter is a significant grain crop in Ethiopia, occupying over 30% of the country's total cultivated land. Tef is known for its long shelf life, drought resistance, natural pest resistance, versatility in food, beverages, and cattle feed. The crop has recently gained popularity in the United States for its nutritional qualities. Tef has a high content of minerals, amino acids, and is also gluten-free. In the context of climate change and the need for sustainable agriculture, exploring alternative crops to ensure food security is crucial. This research focuses on identifying tef accessions that are well-adapted to …

Forming A Comprehensive Pollen Library For The Purpose Of Native Bee Pollen Identification And Analysis, Madeline Roubik, Ai Wen

Summer Undergraduate Research Program (SURP) Symposium

The Iowa prairie is home to many species of bees who collect pollen for nutrition or nesting purposes (Real, 1983). Most bees collect pollen in their scopa, a small pollen collection pouch on their back legs or abdomen. However, the entire bee, including their thorax, can come in contact with pollen. Because of this, it is assumed that a bee’s pollen collection will be more diverse on its thorax where it unintentionally comes into contact with pollen, than its legs where it intentionally collects pollen. In this study, we created a comprehensive pollen library of native Iowa prairie flowers, and …

Investigating Gene Looping In Yeast, Abbie Bangs, Justin Peters

Summer Undergraduate Research Program (SURP) Symposium

Saccharomyces cerevisiae, also known as brewer’s yeast, is the model organism we chose to interrogate a possible length dependence of gene looping. Gene looping occurs when the promoter and the terminator regions of a gene come together in a physical interaction to form a loop. RNA polymerase transcribes the gene at an amplified rate due to the gene loop (Figure 1), but little research exists as to what factors are important for this process, including the mechanics of the underlying DNA.

Molybdenum Disulfide (Mos₂) As A Nanocellulose Substrate, Mariana Torres, Dylan Seiffert, Elise Hong-Miller, T. E. Kidd

Summer Undergraduate Research Program (SURP) Symposium

Cellulose is a substance abundant in nature, found in fruits and vegetables, plant cell walls (National Center for Biotechnology Information, 2023), and wood (Sjostrom, 1993). The abundance of cellulose is attributable to its linear chemical structure, as it is composed of long strands of glucose molecules linked together by hydrogen atoms, which enable the substance to withstand significant pressure without breaking (Rongpipi et al., 2019). This property—known as high tensile strength—is joined by its non-toxicity and sustainability, given cellulose may be degraded and renewed naturally (Kaur, 2021). These various properties indicate versatility, and its resultant current and desired uses—such as …

Flow Through Processing Of Nanocellulose For Space Exploration Applications, Dylan Seiffert, T. E. Kidd

Summer Undergraduate Research Program (SURP) Symposium

Nanocellulose is synthesized from cellulose, which is the most abundant organic molecule found in nature. Nanocellulose solids are biodegradable and have a strength-to-weight ratio eight times that of steel¹. The production of nanocellulose solids is challenging due to the large volume loss that occurs during the curing process as well as the time and cost associated with ultrasonic processing. The loss of volume during curing often results in cracking and deformation of the cured solid. The issues caused by the large volume loss can be mitigated using additives that do not compromise the strength, shape, or machineability of …

Impacts And Applications Of Vegetable Glycerin On Nanocellulose, Elise Hong-Miller, Dylan Seiffert, T. E. Kidd

Summer Undergraduate Research Program (SURP) Symposium

Nanocellulose is created by breaking down cellulose, the most common organic molecule found in nature. Nanocellulose has attractive properties, such as a very high strength-to-weight ratio, for many different applications. A major challenge is that nanocellulose has a very high shrinkage % (~90%) when it is dried, as well as a strong probability to crack. Different additives can be added in varying amounts to either mitigate or intensify these aspects, as well as affect its drying conditions. This investigation is helping the National Aeronautics and Space Administration (NASA) and the U.S. Army to find a use for this highly renewable …

Assessment Of Dna Concentration And Degradation From Extracted Bumble Bee Thorax Muscle 2023, Joel Lado, Ai Wen, Kenneth Elgersma

Summer Undergraduate Research Program (SURP) Symposium

Deoxyribonucleic acid (DNA) is the foundation of an organism's ability to develop, survive, and reproduce. High genomic DNA is imperative for many scientific studies and applications such as Biotechnology, Genetics, & Pharmaceuticals. The separation of genomic DNA once purified from other cellular components (RNA, protein) can be sequenced to study entire genomes, develop antibodies, and limit genetic mutations.

Although a very stable molecule, DNA is susceptible to degradation under normal cellular conditions to various factors. Hence it is essential to properly extract genetic material from bee specimens, to better understand their population genetics.

DNA often needs to be stored for …

Short-Term Improvement In Self-Perceived Knowledge And Weight Bias Following A 15-Week Course On Pediatric Obesity, Rosanna P. Watowicz, Harrine Ramesh

Journal of Dietetic Education

Registered dietitian nutritionists (RDNs)are an integral part of weight management teams; however, previous research indicates that RDNs may hold weight-related biases. Additionally, there may be a gap between what RDNs learn about weight management during their education and training and the competencies that RDNs should achieve before working in weight management. The objective of this study was to examine whether a 15-week graduate course on pediatric obesity led to a short-term increase in self-perceived knowledge of 13 Obesity Medicine Education Collaborative competencies, as well as a short-term decrease in weight bias. Nutrition graduate students enrolled in the course completed a …

The Role Of Adenovirus Serotype 5 E4 11k In The Relocalization Of The Cellular P Body Proteins, Kasey A. Karen, La`Quita Randolph, Kevin Neubrecht, Heather Vincent

Georgia Journal of Science

Human adenoviruses are a useful tool to understand basic cellular biology in addition to viral infections. Historically, cellular splicing was first discovered in adenovirus, but other cellular processes, such as double-strand break repair and aggresome formation, have been further elucidated through adenoviral infection. The adenovirus protein E4 11k has been shown to disrupt cytoplasmic processing bodies (p bodies), which are not well-understood but are involved in mRNA metabolism. Several p body proteins were found to be reorganized in the cytoplasm with adenovirus serotype 5 (Ad5) able to cause the colocalization of these p body proteins with aggresomes. The p body …

Watermark Hiding In Hdr Image Based On Visual Saliency And Tucker Decomposition, Roa'a M. Al-Airaji, Ibtisam A. Aljazaery, Haider Th. Salim Alrikabi

Karbala International Journal of Modern Science

Recently, great attention has been paid to high dynamic range (HDR) images because of their richly detailed and high dynamic range of intensity. The need for pre-processing of the HDR image format with tone mapping (TM) operators makes it unique; the TM operators are provided on display with a low dynamic range (LDR). On the other hand, TM can be regarded as an inevitable attack when protecting HDR image ownership is considered. An adaptive approach for concealing watermarks based on visual saliency and Tucker decomposition has been presented in this article. In the first step, three feature maps were produced …

New Corona Virus Provokes Peripheral Gangrene In Lower Limb Of Type-1 Diabetic Patient: A Case Report, Hussein A. Ghanimi, Diaa K. Abd Ali

Al-Ameed Journal for Medical Research and Health Sciences

Introduction: Corona virus disease 2019 (COVID-19) presents with un-expected complications. Diabetic patients are more susceptible for lower limb complications. Importance: Peripheral gangrene may be associated with COVID-19 in diabetic patients. Case presentation: We described one diabetic patient with COVID-19 associated peripheral gangrene (PG) in lower limb. A 56 years old female patient with type-1 diabetes tested positive for COVID-19. She suffered from PG in her left foot. Hematological, biochemical, Doppler sonography, echocardiography (Echo) and electrocardiogram (ECG) tests were done for diagnosis and evaluation of the case. General checkup indicated presence of an abscess in her foot. Later, abscess drainage surgery …

Advances In Drug Delivery Systems: A Mini-Review, Lina M. Shaker, Ahmed A. Al-Amiery, Abdul Amir H. Kadhum

Al-Ameed Journal for Medical Research and Health Sciences

Drug delivery systems (DDS) refer to technologies designed to improve the safety and efficacy of drugs by controlling their release, absorption, and distribution in the body. DDS has gained significant attention due to its potential to enhance the therapeutic outcomes of various drugs. This mini-review article provides an overview of the current status of DDS. The article begins with a brief introduction to DDS, highlighting its importance in drug development and delivery. It also provides a historical background of DDS to provide context to its current developments. Next, the mini-review focuses on the various types of DDS, including oral, transdermal, …

Pyronaridine As A Bromodomain-Containing Protein 4-N-Terminal Bromodomain (Brd4-Bd1) Inhibitor: In Silico Database Mining, Molecular Docking, And Molecular Dynamics Simulation, Mahmoud A. A. Ibrahim, Mahmoud M. H. Abdelhamid, Khlood A. A. Abdeljawaad, Alaa H. M. Abdelrahman

Faculty Journal Articles

No abstract provided.

Platelet Rich Plasma For Women With Recurrent Implantation Failure Undergoing Frozen Embryo Transfer, Gabriela Ortiz

Yale School of Medicine Physician Associate Program Theses

In vitro fertilization with embryo transfer is often considered a solution for infertile women. The proportion of infertile women who fail to become pregnant after an embryo transfer remains high despite advances in assisted reproductive technologies. Use of autologous platelet-rich plasma may have a beneficial effect on implantation. Our objective is to determine if autologous platelet-rich plasma prior to embryo transfer is effective in improving pregnancy rates among women with recurrent implantation failure. We will design a randomized controlled trial such that women will receive an intrauterine infusion of either autologous platelet-rich plasma or saline 48 hours prior to transferring …

Historical Trends Of Aquatic Invasive Species Introduction And Establishment In Illinois, Usa, Carter S. Cranberg, Reuben Keller

School of Environmental Sustainability: Faculty Publications and Other Works

Tracking the introduction and establishment of aquatic invasive species (AIS) is important for monitoring the biological and economic health of freshwater environments. The state of Illinois (USA) is a critical region for understanding the threats of AIS because it possesses the only continuous aquatic habitat connecting the Laurentian Great Lakes and Mississippi River Basins. In this study, we update a previous effort to catalogue and evaluate historical AIS records from Illinois. Our updated database shows that there are now at least 92 nonindigenous aquatic species established in Illinois and a further 51 have been recorded as introduced but not established. …

Beef Color And Tenderness Response To Production Systems Utilizing Additive Combinations Of Growth-Promotant Technologies, Megan J. Webb, Janna J. Block, John R. Jaeger, R. Funston, Michael G. Gonda, Keith R. Underwood, Judson K. Grubbs, Kenneth C. Olson, Amanda D. Blair

West Central Research and Extension Center, North Platte

The objective of this study was to compare the influence of beef production systems using additive combinations of growth-promotant technologies on meat quality. Steer calves (n = 120) were assigned to 1 of 4 treatments: 1) no technology (NT; control), 2) antibiotic treated (ANT; NT plus therapeutic antibiotics, monensin, and tylosin), 3) implant treated (IMP; ANT plus a series of three implants), and 4) beta-agonist treated (BA; IMP plus ractopamine-HCl). Muscle biopsy samples from the longissimus lumborum were extracted from a subset (n = 4 per treatment) of steers to evaluate expression of calpain-1, calpain-2, and calpastatin using …

Local Tumor Destruction And Liver Resection Increase Overall Survival In Intermediate/Advanced Hepatocellular Carcinoma Patients: Evidence From A Population-Based Study, Yang Zhang, Yi Zhang, Taiyu He, Guangliang Liu, Minjie Duan, Jian Huang, Christy Huang, Scott Lowe, Dazhi Ke, Xiaozhu Liu, Junyi Cao

Student Publications

Background: Liver resection (LR) and local tumor destruction (LTD) are effective treatments, but not commonly recommended for patients with intermediate/advanced hepatocellular carcinoma (HCC). This study aimed to explore whether LR/LTD could improve overall survival (OS) of these patients, and to identify the patients who will most likely benefit from LR/LTD.

Methods: Data of patients with intermediate/advanced HCC between 2001 and 2018 were extracted from Surveillance, Epidemiology, and End Results database. OS was compared between HCC patients who received LR/LTD and those who did not. A nomogram was constructed for predicting OS, and it was then validated.

Results: A total of …

July 28, 2023 Ereporter, University Of Alabama At Birmingham

eReporter (campus newsletter)

No abstract provided.

Subunit Gating Resulting From Individual Protonation Events In Kir2 Channels, Grigory Maksaev, Michael Bründl-Jirout, Anna Stary-Weinzinger, Eva-Maria Zangerl-Plessl, Sun-Joo Lee, Colin G Nichols

2020-Current year OA Pubs

Inwardly rectifying potassium (Kir) channels open at the 'helix bundle crossing' (HBC), formed by the M2 helices at the cytoplasmic end of the transmembrane pore. Introduced negative charges at the HBC (G178D) in Kir2.2 channels forces opening, allowing pore wetting and free movement of permeant ions between the cytoplasm and the inner cavity. Single-channel recordings reveal striking, pH-dependent, subconductance behaviors in G178D (or G178E and equivalent Kir2.1[G177E]) mutant channels, with well-resolved non-cooperative subconductance levels. Decreasing cytoplasmic pH shifts the probability towards lower conductance levels. Molecular dynamics simulations show how protonation of Kir2.2[G178D], or the D173 pore-lining residues, changes solvation, K

Light Alters Leaf Breakdown Rates And Metabolic Activity In Anthropogenically Impacted Nebraska Streams, William Reilly Farrell

School of Natural Resources: Dissertations, Theses, and Student Research

Algal growth influences carbon breakdown rates in aquatic ecosystems; however, the magnitude and direction of these effects differ based on ecosystem context. To explore algal priming effects on decomposition in anthropogenically impacted ecosystems, I conducted light manipulation experiments in three streams, each surrounded by different land uses (i.e., predominantly urban, row crop, or concentrated animal feeding operation) across Nebraska. I measured leaf breakdown rates, metabolic activity, fungal and algal biomass, and leaf percent carbon (C) and phosphorus (P) to assess the effect of light differences. These streams were impacted by sedimentation and turbidity over the course of the leaf incubation. …

Pronghorn Survival And Resource Selection In Western Nebraska's Agriculturally Dominated Landscape, Katie Piecora

School of Natural Resources: Dissertations, Theses, and Student Research

Nebraska’s pronghorn population has been stable over the last decade, yet their presence on the landscape remains a contentious subject amongst private landowners. Conversion of grassland for crop production and increased anthropogenic activity has drastically altered pronghorn behavior throughout their current range, however basic ecology and resource use by pronghorn in Nebraska remains poorly understood. Establishing baseline population metrics and seasonal patterns of resource use for this population at the eastern periphery of the species range is critical to guide management actions. We deployed GPS collars on 110 adult pronghorn to quantify survival, mortality risk, and seasonal resource selection in …

N-Acetyltransferase 9 Ameliorates Aβ42-Mediated Neurodegeneration In The Drosophila Eye, Prajakta Deshpande, Anuradha Venkatakrishnan Chimata, Emily E. Snider, Aditi Singh, Madhuri Kango-Singh, Amit Singh

Biology Faculty Publications

Alzheimer’s disease (AD), a progressive neurodegenerative disorder, manifests as accumulation of amyloid-beta-42 (Aβ42) plaques and intracellular accumulation of neurofibrillary tangles (NFTs) that results in microtubule destabilization. Targeted expression of human Aβ42 (GMR > Aβ42) in developing Drosophila eye retinal neurons results in Aβ42 plaque(s) and mimics AD-like extensive neurodegeneration. However, there remains a gap in our understanding of the underlying mechanism(s) for Aβ42-mediated neurodegeneration. To address this gap in information, we conducted a forward genetic screen, and identified N-acetyltransferase 9 (Mnat9) as a genetic modifier of GMR > Aβ42 neurodegenerative phenotype. Mnat9 is known to stabilize microtubules by inhibiting c-Jun-N- …

The Effects Of Ultra-Marathon Trail Running And The Physiological Response Of Stress On Salivary Biomarkers, Kevan W. Stout, Jeremy R. Pearson, Catherine A. Hambleton, Jake A. Deckert, Philip M. Gallagher

Journal of Human Performance in Extreme Environments

PURPOSE: This study investigated changes in salivary α-amylase (sAA), cortisol, and interleukin-1β (IL-1β) concentrations following completion of an ultra-marathon trail run (UMT) to better understand the physiological stressors imposed by this extreme type of race. METHODS: Eight subjects participated in this study. Each subject completed a 50 km UMT. Two-minute oral salivary swabs were taken 10 minutes prior to race start and again within 1 minute of race finish. Samples were analyzed for sAA, cortisol and IL-1β using ELISA kits. RESULTS: Our results demonstrated a significant increase in both sAA (p = 0.002) and cortisol (p < 0.001) from baseline. No significant differences were observed for IL-1β. There was no significant relationship between the observed sAA increase and race speed. The observed increase in cortisol was significantly correlated with race speed (R² = 0.582, p …

Optimizing The Timing Of Biologic And Surgical Therapy For Patients With Refractory Chronic Rhinosinusitis With Nasal Polyposis (Crswnp), Emily Garvey, Bita Naimi, Alexander Duffy, Paavali Hannikainen, Chase Kahn, Douglas Farquhar, Marc Rosen, Mindy R. Rabinowitz, Elina Toskala, Gurston G. Nyquist

Department of Otolaryngology - Head and Neck Surgery Faculty Papers

INTRODUCTION: Chronic rhinosinusitis with nasal polyposis (CRSwNP) is often treated with endoscopic sinus surgery (ESS); however, patients may require revision surgery due to recurrence. To date, no studies have compared outcomes for combined surgery and biologic therapy for CRSwNP compared with biologic therapy alone.

METHODS: Retrospective case-control study of CRSwNP patients who underwent ESS while on dupilumab or mepolizumab (ESS-biologic cohort) compared with CRSwNP patients on biologic therapy (biologic-only controls). Cohorts were matched according to indication, aspirin-exacerbated respiratory disease (AERD), sinonasal outcome test-22 (SNOT-22), and total polyp scores.

RESULTS: Sixteen patients underwent ESS while on biologic therapy (13 dupilumab and …

Annotation Of Non-Model Species’ Genomes, Taiya Jarva

Master's Theses

The innovations in high throughput sequencing technologies in recent decades has allowed unprecedented examination and characterization of the genetic make-up of both model and non-model species, which has led to a surge in the use of genomics in fields which were previously considered unfeasible. These advances have greatly expanded the realm of possibilities in the fields of ecology and conservation. It is now possible to the identification of large cohorts of genetic markers, including single nucleotide polymorphisms (SNPs) and larger structural variants, as well as signatures of selection and local adaptation. Markers can be used to identify species, define population …