Digital Commons Network^™

Elucidating The Architecture Of The Tclijn Complex That Converts Cysteine To Thiazoles In The Biosynthesis Of Micrococcin, Diana G. Calvopina Chavez

Theses and Dissertations

Thiopeptides are a family of antimicrobial peptides that are characterized for having sulfur-containing heterocycles, and for being highly post-translationally modified. Numerous thiopeptides have been identified; almost all of which inhibit protein synthesis in gram-positive bacteria. These intrinsic antimicrobial properties make thiopeptides promising candidates for the development of new antibiotics. The antimicrobial peptide micrococcin is a thiopeptide that is synthesized by the ribosome and undergoes several post-translational modifications (PTMs). Micrococcin is formed from a precursor peptide, TclE. TclE comprises an N-terminal leader (35-AA) that is crucial for recognition of the PTM machinery, alongside a C-terminal core sequence (14-AA) that undergoes multiple …

Radiofrequency Inactivation Of Salmonella In Black Pepper And Dried Basil Leaves Using In-Package Steaming, Surabhi Wason, Rossana Villa-Rojas, Jeyamkondan Subbiah

Department of Food Science and Technology: Faculty Publications

Radiofrequency (RF) heating has been extensively studied for pasteurizing low‐moisture foods. Currently, bulk foods are treated with radiofrequency; potential cross‐contamination may occur during packaging of pasteurized products. As an alternative, in‐package RF processing was evaluated for Salmonella inactivation on black peppercorns and dried basil leaves and prevention of cross‐contamination during storage postprocessing. Inpackage steaming refers to the process in which the samples were heated in a steam vent package to generate and retain steam during the treatment. This treatment achieved good heating uniformity which could be because of the circulation of steam within the package. One‐way steam vent allowed the …

Lipid Membranes-Mediated Interactions And Self-Assembly Of Spherocylindrical Nanoparticles, Abash Sharma

Electronic Theses and Dissertations Archive

This dissertation highlights the pivotal role of nanoparticles (NPs) anisotropy on their adhesion and potential self-assembly on lipid membranes. The research, which is performed in the context of spherocylindrical NPs (SCNPs), offers valuable insights into the complex interaction between NPs and lipid membranes and the utilization of lipid membranes as a medium for SCNPs self-assembly, potentially opening the door to various advanced applications. Modeling SNCPs as triangulated hollow shells, the adhesion modes and endocytosis of SCNPs on tensionless planar membranes are investigated using molecular dynamics simulations of a coarse-grained implicit-solvent model. The SCNPs are shown to adhere to the membrane …

Trend Of Outcome Metrics In Recent Out-Of-Hospital-Cardiac-Arrest Research: A Narrative Review Of Clinical Trials, Natalie N Htet, Daniel Jafari, Jennifer A Walker, Ali Pourmand, Anna Shaw, Khai Dinh, Quincy K Tran

Faculty, Staff and Student Publications

Cardiopulmonary resuscitation (CPR) research traditionally focuses on survival. In 2018, the International Liaison Committee on Resuscitation (ILCOR) proposed more patient-centered outcomes. Our narrative review assessed clinical trials after 2018 to identify the trends of outcome metrics in the field OHCA research. We performed a search of the PubMed database from 1 January 2019 to 22 September 2023. Prospective clinical trials involving adult humans were eligible. Studies that did not report any patient-related outcomes or were not available in full-text or English language were excluded. The articles were assessed for demographic information and primary and secondary outcomes. We included 89 studies …

Eicosanoid And Eicosanoid-Related Inflammatory Mediators And Exercise Intolerance In Heart Failure With Preserved Ejection Fraction, Emily S Lau, Athar Roshandelpoor, Shahrooz Zarbafian, Dongyu Wang, James S Guseh, Norrina Allen, Vinithra Varadarajan, Matthew Nayor, Ravi V Shah, Joao A C Lima, Sanjiv J Shah, Bing Yu, Mona Alotaibi, Susan Cheng, Mohit Jain, Gregory D Lewis, Jennifer E Ho

Faculty, Staff and Student Publications

Systemic inflammation has been implicated in the pathobiology of heart failure with preserved ejection fraction (HFpEF). Here, we examine the association of upstream mediators of inflammation as ascertained by fatty-acid derived eicosanoid and eicosanoid-related metabolites with HFpEF status and exercise manifestations of HFpEF. Among 510 participants with chronic dyspnea and preserved LVEF who underwent invasive cardiopulmonary exercise testing, we find that 70 of 890 eicosanoid and related metabolites are associated with HFpEF status, including 17 named and 53 putative eicosanoids (FDR q-value < 0.1). Prostaglandin (15R-PGF2α, 11ß-dhk-PGF2α) and linoleic acid derivatives (12,13 EpOME) are associated with greater odds of HFpEF, while epoxides (8(9)-EpETE), docosanoids (13,14-DiHDPA), and oxylipins (12-OPDA) are associated with lower odds of HFpEF. Among 70 metabolites, 18 are associated with future development of heart failure in the community. Pro- and anti-inflammatory eicosanoid and related metabolites may contribute to the pathogenesis of HFpEF and serve as potential targets for intervention.

Increased Igfbp2 Levels By Placenta-Derived Mesenchymal Stem Cells Enhance Glucose Metabolism In A Taa-Injured Rat Model Via Ampk Signaling Pathway, Dae-Hyun Lee, Hyeri Park, Jun-Hyeong You, Jin Seok, Dong-Wook Kwon, Young-Ran Kim, Gi-Jin Kim

Faculty, Staff and Student Publications

The insulin resistance caused by impaired glucose metabolism induces ovarian dysfunction due to the central importance of glucose as a source of energy. However, the research on glucose metabolism in the ovaries is still lacking. The objectives of this study were to analyze the effect of PD-MSCs on glucose metabolism through IGFBP2-AMPK signaling and to investigate the correlation between glucose metabolism and ovarian function. Thioacetamide (TAA) was used to construct a rat injury model. PD-MSCs were transplanted into the tail vein (2 × 10

Evaluation Of The Us Covid-19 Scenario Modeling Hub For Informing Pandemic Response Under Uncertainty, Emily Howerton, Lucie Contamin, Luke C Mullany, Michelle Qin, Nicholas G Reich, Samantha Bents, Rebecca K Borchering, Sung-Mok Jung, Sara L Loo, Claire P Smith, John Levander, Jessica Kerr, J Espino, Willem G Van Panhuis, Harry Hochheiser, Marta Galanti, Teresa Yamana, Sen Pei, Jeffrey Shaman, Kaitlin Rainwater-Lovett, Matt Kinsey, Kate Tallaksen, Shelby Wilson, Lauren Shin, Joseph C Lemaitre, Joshua Kaminsky, Juan Dent Hulse, Elizabeth C Lee, Clifton D Mckee, Alison Hill, Dean Karlen, Matteo Chinazzi, Jessica T Davis, Kunpeng Mu, Xinyue Xiong, Ana Pastore Y Piontti, Alessandro Vespignani, Erik T Rosenstrom, Julie S Ivy, Maria E Mayorga, Julie L Swann, Guido España, Sean Cavany, Sean Moore, Alex Perkins, Thomas Hladish, Alexander Pillai, Kok Ben Toh, Ira Longini, Shi Chen, Rajib Paul, Daniel Janies, Jean-Claude Thill, Anass Bouchnita, Kaiming Bi, Michael Lachmann, Spencer J Fox, Lauren Ancel Meyers, Ajitesh Srivastava, Przemyslaw Porebski, Srini Venkatramanan, Aniruddha Adiga, Bryan Lewis, Brian Klahn, Joseph Outten, Benjamin Hurt, Jiangzhuo Chen, Henning Mortveit, Amanda Wilson, Madhav Marathe, Stefan Hoops, Parantapa Bhattacharya, Dustin Machi, Betsy L Cadwell, Jessica M Healy, Rachel B Slayton, Michael A Johansson, Matthew Biggerstaff, Shaun Truelove, Michael C Runge, Katriona Shea, Cécile Viboud, Justin Lessler

Faculty, Staff and Student Publications

Our ability to forecast epidemics far into the future is constrained by the many complexities of disease systems. Realistic longer-term projections may, however, be possible under well-defined scenarios that specify the future state of critical epidemic drivers. Since December 2020, the U.S. COVID-19 Scenario Modeling Hub (SMH) has convened multiple modeling teams to make months ahead projections of SARS-CoV-2 burden, totaling nearly 1.8 million national and state-level projections. Here, we find SMH performance varied widely as a function of both scenario validity and model calibration. We show scenarios remained close to reality for 22 weeks on average before the arrival …

7 Cups Therapy, Allie Mccutcheon, Jasmine Awad

High Impact Practices Student Showcase Fall 2023

The purpose of this project is to showcase our service learning experiences from volunteering with 7 cups. 7 Cups is a therapy website that provides free emotional support and talk therapy to those who really need that help. Jasmine and I created a 7 cups listener account where we would log onto the 7 cups portal and find a member who needs help. This whole website is all anonymous in order to protect the listeners and the members identities. Jasmine and I would chat with members and figure out what seems to be troubling them and how to combat that …

Examining The Role Of Cteg Using Drosophila, Jadaya V. Davis, Om Patel, Sebastian Velez

High Impact Practices Student Showcase Fall 2023

This project focused on creating viable drosophila models that could be used to evaluate and understand the significance of the N-tarp portion in the effector genes of Chlamydia Trachomatis. During our project we performed multiple DNA clean ups, PCR's, gel electrophoresis, double digestions, and DNA ligations. In this we learned not only the methodological importance within these laboratory techniques but that a great deal of learning occurs in being able to evaluate and analyze ways to troubleshoot when intended results from an experiment are not observed.

Ageism By A Community Sample Of Young Adults: Expanding The Contact Hypothesis To Explore Ageism, Helena Lucia Swanson

College of Science and Health Theses and Dissertations

Aging demographics globally are changing rapidly, resulting in the category of “older adults” (65 years and older) growing to eventually become the largest age demographic (United Nations, 2022). As our world gets older, ageism is a growing concern given its health, societal, cultural, and political consequences (Chrisler et al., 2016; Bugental & Hehman, 2007; Levy et al., 2020). Following Allport’s Contact Hypothesis (1954), this dissertation empirically analyzed predictors of ageism, including contact with older adults, for younger adults (18-26 years old) residing in U.S. communities. Participants were recruited using Prolific Academic, an online crowdsourcing platform and responded to five scales …

Supporting Novice Nurse Transition To Practice Through Mentorship, Jesssica M. Cox

Walden Dissertations and Doctoral Studies

No abstract provided.

Volume Cxliii, Number 8, November 10, 2023, Lawrence University

The Lawrentian

No abstract provided.

A Network Pharmacology And Molecular-Docking-Based Approach To Identify The Probable Targets Of Short-Chain Fatty-Acid-Producing Microbial Metabolites Against Kidney Cancer And Inflammation, Md Rezaul Karim, Md Niaj Morshed, Safia Iqbal, Shahnawaz Mohammad, Ramya Mathiyalagan, Deok Chun Yang, Yeon Ju Kim, Joon Hyun Song, Dong Uk Yang

Faculty, Staff and Student Publications

(1) Background: A large and diverse microbial population exists in the human intestinal tract, which supports gut homeostasis and the health of the host. Short-chain fatty acid (SCFA)-secreting microbes also generate several metabolites with favorable regulatory effects on various malignancies and immunological inflammations. The involvement of intestinal SCFAs in kidney diseases, such as various kidney malignancies and inflammations, has emerged as a fascinating area of study in recent years. However, the mechanisms of SCFAs and other metabolites produced by SCFA-producing bacteria against kidney cancer and inflammation have not yet been investigated. (2) Methods: We considered 177 different SCFA-producing microbial species …

Root-Associated Bacterial Communities And Root Metabolite Composition Are Linked To Nitrogen Use Efficiency In Sorghum, Yen Ning Chai, Yunhui Qi, Emily Goren, Dawn Chiniquy, Amy M. Sheflin,, Susannah G. Tringe, Jessica E. Prenni, Peng Liu, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

The development of cereal crops with high nitrogen use efficiency (NUE) is a priority for worldwide agriculture. In addition to conventional plant breeding and genetic engineering, the use of the plant microbiome offers another approach to improving crop NUE. To gain insight into the bacterial communities associated with sorghum lines that differ in NUE, a field experiment was designed comparing 24 diverse Sorghum bicolor lines under sufficient and deficient nitrogen (N). Amplicon sequencing and untargeted gas chromatography–mass spectrometry were used to characterize the bacterial communities and the root metabolome associated with sorghum genotypes varying in sensitivity to low N. We …

Algorithmic Design In Recommender Systems: Expanding Dbe Participation In The U.S. Construction Industry, Dick C.E. Davis Ii

USF Tampa Graduate Theses and Dissertations

The multifaceted U.S. construction environment, characterized by vast projects and intricate agreements, is anchored in trust, exemplified by bonding. For Disadvantaged Business Enterprises (DBEs), the bonding process can be challenging.

This dissertation provides an in-depth evaluation of the "System for facilitating a project between contractors and owners," a patented methodology innovatively developed by my father, Dr. Dick Davis, Sr. and later approved through my research efforts, as indicated by the patent number US8346582B1. The system adeptly gathers and processes data from owners, projects, and contractors. Despite its proficiency in managing extensive contractor and project data, the system faces challenges in …

Tert Immunohistochemistry As A Surrogate Marker For Tert Promoter Mutations In Infiltrating Gliomas, Gillian Gordon Perue, Hao Ying, Antonio Bustillo, Lili Zhou, Carolina M Gutierrez, Hannah E Gardener, Judith Krigman, Angus Jameson, Chuanhui Dong, Tatjana Rundek, David Z Rose, Jose G Romano, Ayham Alkhachroum, Ralph L Sacco, Negar Asdaghi, Sebastian Koch

Faculty, Staff and Student Publications

BACKGROUND: Guideline-based hypertension management is integral to the prevention of stroke. We examine trends in antihypertensive medications prescribed after stroke and assess how well a prescriber's blood pressure (BP) medication choice adheres to clinical practice guidelines (BP-guideline adherence).

METHODS AND RESULTS: The FSR (Florida Stroke Registry) uses statewide data prospectively collected for all acute stroke admissions. Based on established guidelines, we defined optimal BP-guideline adherence using the following hierarchy of rules: (1) use of an angiotensin-converting enzyme inhibitor or angiotensin receptor blocker as first-line antihypertensive among diabetics; (2) use of thiazide-type diuretics or calcium channel blockers among Black patients; (3) …

Variations And Similarities In Trumpet And Vocal Pedagogy, Ryn Jenkins

Honors College Theses

While both disciplines fall under the umbrella of professional music performance, trumpet and vocal pedagogy and performance are typically considered to be separate entities. Even though, historically, the two have impacted each other, very few materials evaluate these subjects in tandem. That is what this thesis proposes to do. In examining the two different disciplines, I will explore their similarities and differences, and how they can impact students and teachers today.

There are many benefits to be gained from studying trumpet as well as voice, but along with those benefits come pitfalls that students can easily fall into. In order …

Merging Acceptance And Commitment Therapy With Exposure Exercises To Treat Social Anxiety In A Teen, Caleb D. Farley, Michael P. Twohig

Psychology Faculty Publications

Social anxiety disorder (SAD) often develops during teenager years, and it is important to conceptualize developmentally appropriate interventions. Exposure therapy framed from a perspective of acceptance and commitment therapy (ACT) shows promise for decreasing pathology and increasing wellbeing. This case illustrates the process through which exposure therapy was integrated with ACT to elicit meaningful outcomes in a case of SAD with a 16-year-old female. Treatment outcomes assessed included engagement in values-based activities as well as assessments of depression, social anxiety, separation anxiety, generalized anxiety, experiential avoidance, and contextually targeted school-based wellbeing. Intervention centered on learning ACT principles through relatable metaphors …

Uncovering Temporal Dynamics In Traffic Safety: Application Of Duration-Based Models For Diagnostic And Predictive Analysis, Diwas Thapa

Electronic Theses and Dissertations Archive

This research consists of four studies aimed at enhancing traffic safety. These studies employ innovative approaches and model frameworks based on duration models. The first study focuses on Work Zone Intrusion Alert Systems (WZIAS) and explores their component layout and impact on work zone safety. By analyzing the duration between work zone intrusion and crashes, this study identifies critical factors contributing to work zone crashes when WZIAS are in use. The study concludes by offering recommendations to optimize WZIAS effectiveness, including potential adjustments to existing work zone guidelines. The second study addresses the limitations of duration models in handling time-varying …

Factors Associated With Urban High School Graduation Rates In Tennessee, Keith Hembree

Electronic Theses and Dissertations Archive

This study aimed to investigate the relationships, if any, between Ready to Graduate indicator, average ACT scores, chronic absenteeism rates, and school counselor to student ratios in Tennessee's four urban counties surrounding Memphis, Nashville, Chattanooga, and Knoxville, Tennessee. Only eight of Tennessee's 95 counties have a population that is at least 75% urban, according to the Tennessee Advisory Commission on Intergovernmental Relations 2016 Report. The existence of segregated schools inside urban regions and the opportunity gap suggests that more research and data about Tennessee's urban communities are needed, especially in relation to graduation rates. The data analyzed for this study …

A Spatial Data Analysis Of Tennessee Accountability School Designations (Tasd) And Their Social, Economic, And Academic Make-Up, Taylor Ray Tartera

Electronic Theses and Dissertations Archive

This study looks closely at geography in education and the impact it has on the schools in Memphis, Tennessee. The location of a school can directly correlates with how successful the school will be- both with proficient test scores as well as funding and support from community stakeholders. Specific schools in Tennessee receive Tennessee Accountability School Designations (TASD). These designations are given to the highest and lowest-performing K-12 public schools in Tennessee. Memphis is Tennessee’s largest school district with a list of 36 schools who received a TASD for low-performing; while on the contrary, 44 schools earned a TASD for …

Prediagnostic Selenium Status, Selenoprotein Gene Variants And Association With Breast Cancer Risk In A European Cohort Study, David J Hughes, Lutz Schomburg, Mazda Jenab, Carine Biessy, Catherine Méplan, Aurelie Moskal, Qian Sun, Kamil Demircan, Veronika Fedirko, Elisabete Weiderpass, Maryam Mukhtar, Anja Olsen, Anne Tjønneland, Kim Overvad, Matthias Schulze, Therese Haugdahl Nøst, Guri Skeie, Karina Standahl Olsen, Fulvio Ricceri, Sara Grioni, Domenico Palli, Giovanna Masala, Rosario Tumino, Fabrizio Pasanisi, Pilar Amiano, Sandra M Colorado Yohar, Antonio Agudo, Maria-Jose Sánchez, Eva Ardanaz, Malin Sund, Anne Andersson, Aurora Perez-Cornago, Ruth Travis, Alicia K Heath, Laure Dossus

Faculty, Staff and Student Publications

Selenium (Se) may help prevent breast cancer (BC) development. Owing to limited observational evidence, we investigated whether prediagnostic Se status and/or variants in the selenoprotein genes are associated with BC risk in a large European cohort. Se status was assessed by plasma measures of Se and its major circulating proteins, selenoprotein P (SELENOP) and glutathione peroxidase 3 (GPX3), in matched BC case-control pairs (2208 for SELENOP; 1785 for GPX3 and Se) nested within the European Prospective Investigation into Cancer and Nutrition (EPIC). Single nucleotide polymorphisms (SNPs, n = 452) in 55 selenoprotein and Se metabolic pathway genes and an additional …

Drug Court Professionals’ Perspectives On Marijuana Legalization Implications For The Drug Court System, Daniel Akwasi Amankwaah

Walden Dissertations and Doctoral Studies

No abstract provided.

Supporting Entry-Level Staff In Managing Behaviors In Children With Autism Spectrum Disorder In A Respite Care Program, Brittany Anderson

Walden Dissertations and Doctoral Studies

No abstract provided.

Policy Research On Allocation Of Science And Technology Funds For State Key Laboratory After Reorganization, Bo Zhang, Xue Wang, Congli Sun

Bulletin of Chinese Academy of Sciences (Chinese Version)

The state key laboratory is an important component of the national strategic scientific and technological strength. The CPC Central Committee made a major decision to reorganize the state key laboratory system, proposed to increase investment in the reorganized state key laboratory, and to reform support policies. Aiming to achieve sci-tech self-reliance and self-strengthening at higher levels, studying the funds allocation of state key laboratory in the new era has important practical and far-reaching significance. In the previous practice, China’s allocation of funds for state key laboratory has 4 problems, such as weak correlation between fund allocation and the mission orientation …

Probe Into Mental Health Issues Caused By Major Natural Disasters And Crisis Interventions, Yankun Sun, Yimiao Gong, Xuebing Huang, Ying Qian, Siwei Sun, Hongguang Chen, Lin Lu

Bulletin of Chinese Academy of Sciences (Chinese Version)

Major natural disasters not only cause severe personnel casualties and social losses, but also bring long-lasting mental health issues related to psychological trauma. Timely and efficient post-disaster crisis interventions can avoid indelible psychological damages that disasters bring to populations such as survivors, victims’ families, witnesses, and rescuers. This article overviews the impact of major natural disasters on mental health and the characteristics of post-disaster psychological crisis. It also summarizes previous experience in post-disaster crisis interventions. In addition, it probes into future post-disaster crisis interventions, including improvement of the legal system for crisis intervention, clarification of the role of the government …

The 14-Day Rule For In Vitro Human Embryo Research Requires Adjustment: From A Comprehensive Perspective Of Science, Ethics And Policy Research, Xiao Lu, Ming Zhao, Huihui Liu, Tianqing Li, Tao Tan, Weizhi Ji

Bulletin of Chinese Academy of Sciences (Chinese Version)

Since it was proposed, in vitro human embryo research had been following the 14-day rule, which limited in vitro research on human embryos within 14 days after fertilization. With the advancement in embryo culturing technology, this ethical rule has been greatly challenged. In 2021, the International Society for Stem Cell Research (ISSCR) recommended a conditional extension of the existing time limit in ISSCR Guidelines for Stem Cell Research and Clinical Translation, hence the international scientific community has restarted the debate on the 14-day rule. This study systematically analyzed the historical background and controversies of the 14-day rule ethical regulation of …

2023 Issue 11 Content

Bulletin of Chinese Academy of Sciences (Chinese Version)

No abstract provided.

Potential Therapeutic Target Found To Combat Tuberculosis, A Disrupted Nad(H) Homeostasis, Public Relations

Browse All News

No abstract provided.

Exploring Pathways To Food Science Careers In Southern California: A Case Study In Food Science Career Development, Jeremy L. Hsu, Sarah Ahles, Lilian W. Senger, Anuradha Prakash

Food Science Faculty Articles and Research

Southern California is a diverse region that is home to a high concentration of food science companies, with an increasing demand for additional food scientists and technologists to join this workforce. Despite this abundance of food science companies and the high demand for jobs, there is currently a shortage in the number of qualified food scientists and technologists in the region. This shortage is also observed within higher education, with declining enrollments in the food science graduate and undergraduate programs across Southern California. Here, we conduct a case study to explore the factors that influence students from Southern California to …