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Synthesis Of 6,6- And 7,7-Difluoro-1-Acetamidopyrrolizidines And Their Oxidation Catalyzed By The Nonheme Fe Oxygenase Lolo, Nabin Panth

Theses and Dissertations--Chemistry

One of the remarkable steps in loline alkaloid biosynthesis is the installation of an ether bridge between two unactivated C atoms in 1-exo-acetamidopyrrolizidine (AcAP). LolO, a 2-oxoglutarate-dependent nonheme Fe oxygenase, catalyzes both the hydroxylation of AcAP and the resulting alcohol's cycloetherification to give N-acetylnornoline (NANL). The mechanism of hydroxylation is well understood, but the mechanism of the oxacyclization is not. I synthesized difluorinated analogs of AcAP in an attempt to further understand the mechanism of the unusual cycloetherification step.

I prepared 6,6-F₂-AcAP in eight steps from N,O-protected 4-oxoproline. The key step was a Dieckmann …

Mass Spectrometric Analysis Of Lignin Model Compounds: Fundamental Investigations Of Ionization And Supramolecular Interactions For Lignocellulosic Biomass Applications, Kimberly Dean

Theses and Dissertations--Chemistry

Lignocellulosic biomass is pivotal for the development of renewable energy sources and materials essential to mitigate the exploitation of fossil fuels causing climate change and environmental pollution issues. The conversion of biomass into fuel requires the hydrolysis of cellulose and a biproduct of this process is the isolation of millions of tons of lignin as biorefinery waste. Lignin is a complex high molecular weight polymer whose structure remains undefined and critically limits potential industrial applications of lignocellulosic biomass. The advancement of analytical methods for structural elucidation of lignin and its ensemble of phenolic compounds is therefore essential to advance this …

The Role Of Charge On Dna Packaging And Integrity Within Reconstituted Peptide-Dna Assemblies, Ehigbai Oikeh

Theses and Dissertations--Chemistry

In nature, DNA exists primarily in a highly compacted form. The compaction of DNA in vivo is mediated by cationic proteins; histone in somatic nuclei and arginine-rich peptides called protamines in sperm chromatin. The packaging in the sperm nucleus is significantly higher than somatic nuclei resulting in a final volume roughly 1/20^th that of a somatic nucleus. This tight packaging results in a near crystalline packaging of the DNA helices. While the dense packaging of DNA in sperm nuclei is considered essential for both efficient genetic delivery as well as DNA protection against damage by mutagens and oxidative species, …

Development Of Fluorescence Based Approaches To Understand Astrocyte Biology In The Context Of Nicotine And Nicotinic Receptor Activity, Surya P. Aryal

Theses and Dissertations--Chemistry

Smoking and tobacco use (STU) is a major global health problem and worldwide more than six million people die due to tobacco related diseases each year. Although majority of smokers try to quit smoking several times in their life, traditional therapeutic approaches, which focus only on neuronal cells, have a very low success rate. Understanding the effect of nicotine on glial cells, synaptic communication and blood vasculature in the brain can provide further insights on the neurobiology of substance abuse and can potentially help to design better therapeutic approaches. Glial cells are non-excitable cells in the brain which do not …

Application Of Mass Spectrometry For Characterization Of Plant-Based Phenolics And Alkaloids, Masoumeh Dorrani

Theses and Dissertations--Chemistry

Plant-derived compounds have the potential to produce value-added compounds with a variety of applications. For example, the lignin part of the lignocellulosic biomass, produced in large quantities as waste from the paper and pulp industries, is a rich source of phenolics with potential applications in the renewable energy sector, pharmaceutical, and chemical industries. On the other hand, plant alkaloids are the primary source for developing plant-derived therapeutics. Unfortunately, the recalcitrant nature of plant cell walls, low extraction yields of small secondary metabolites, and the lack of effective analytical methods for a rapid and accurate identification of plant-based compounds and plant’s …

Nanomaterial Synthesis And Real Time Investigation Of Thermal Effects On Nanomaterial And Nano-Interfaces For Real World Applications, Rose H. Pham

Theses and Dissertations--Chemistry

As interest in nanomaterials and nanotechnology continues to grow, so does the need for more efficient and economical synthesis methods to keep up with the demand. The applications for nanomaterials are seemingly endless as they have functions in energy, biomedical, environmental, and many more. Working to develop different morphologies and sizes of nanomaterials will further help expand its utility. With the use of advanced characterization techniques such as in situ transmission electron microscopy (TEM), real time studies on the effects of external forces on nanomaterials are possible under controlled environments. This will give insight on how nanomaterials will perform in …

Natural Carbon Sequestration In The Commonwealth, Carbon Sequestration Task Force, Virginia Coastal Policy Center, William & Mary Law School, Virginia Institute Of Marine Science

Virginia Coastal Policy Center

The Virginia General Assembly created the Carbon Sequestration Task Force in 2021 to examine the feasibility and potential to increase carbon sequestration in the Commonwealth. Specifically, the Task Force must (i) consider possible methods of increasing carbon sequestration within the natural environment through state land and marine resources use policies; agricultural, aquacultural, and silvicultural practices; and other practices to achieve natural resources restoration and long-term conservation; (ii) recommend short-term and long-term benchmarks for increasing carbon sequestration; (iii) develop a standardized methodology to establish baseline carbon levels and account for increases in carbon sequestration over time; (iv) identify existing carbon markets …

Organizational Implications Of Pope Francis’ Integral Ecology, Frank J. Barrett, Ryan G. Duns

Theology Faculty Research and Publications

We explore Pope Francis's “integral ecology” in the encyclical Laudato Si (Francis, 2015) as it provides us with an agenda for a planetary virtue ethic that should inspire the field of Organizational Development to reconsider the moral implications of our work. We begin by offering the framework of virtue ethics as a way of understanding Laudato Si (LS). We then summarize the argument in LS as it focuses on four ecological issues—climate change, pollution, water, and the plight of the poor as we tease out the document's implicit virtue ethic. Finally, we propose how OD practitioners can become more aware …

Venerating Earth: Three Sacramental Perspectives, Jame Schaefer

Theology Faculty Research and Publications

three prominent ways in which the sacramentality of creation has been nuanced over the centuries are explored: (1) Experiencing the presence of God in the world with focus on Ignatius of Loyola’s final contemplation in his Spiritual Exercises; (2) reflecting on manifestations of God’s goodness, power and wisdom that eminent patristic and medieval theologians discerned when studying the world and novel attributes that are discernible today when informed by current scientific findings; and (3) receiving the Eucharist as a heightened encounter with God that can strengthen individuals and communities to act cooperatively. These three ways of perceiving the world within …

On Foxes And Hedgehogs, Roger P. Alford

Journal Articles

This Article is about John Nagle’s many means to one great end. It will outline the many themes of his scholarship: (i) environmental law, (ii) statutory interpretation, (iii) constitutional law, (iv) nuisance and pollution, (v) election law and campaign finance, (vi) Christianity and the environment, and (vii) national parks. It will offer conclusions on how he used his scholarly interests as a means to pursue his overarching worldview.

The Ecology And Dispersal Of The Avian Vampire Fly In Its Native And Invasive Ranges, Carly M. Crow

Graduate Research Theses & Dissertations

Invasive species are considered a leading threat to global biodiversity. There are three major steps in a successful invasion: 1. A species arrives to a non-native location 2. It establishes a breeding population 3. It spreads beyond the location of arrival. The need to understand what variables facilitate a successful invasion across these steps is pressing, especially when considering systems that are vulnerable to biodiversity loss such as islands. The first chapter of this thesis focuses on the arrival step of an invasion. I present research on insects found on a tourist boat traveling between islands in the Galapagos archipelago. …

Grassland Bird Responses To Bison And Prescribed Fire Disturbances At Nachusa Grasslands And Kankakee Sands, Antonio Del Valle

Graduate Research Theses & Dissertations

Grazing from native herbivores such as bison (Bison bison), in combination with prescribed fire, are applied to tallgrass prairies by managers to recreate important disturbance patterns in this ecosystem. Bird communities may be indirectly impacted by these disturbances through their direct impact on plants that provides critical breeding habitat for grassland birds. The objectives of this research are to determine the impacts that bison and prescribed fire have on grassland breeding birds in two tallgrass prairie preserves, Kankakee Sands and Nachusa Grasslands. Birds, vegetation structure, and bison activity were surveyed systematically at these two preserves in 2020 and 2021. Prescribed …

A Phenomenological Study Of Individuals Who Practice A Religion Based On Yorùbá Traditions And Have Formally Engaged In Professional Counseling, Jamille J. Edwards

Graduate Research Theses & Dissertations

This qualitative study explored the lived experiences of individuals who practice a religion based on Yorùbá traditions and have formally engaged in professional counseling. There are a variety of indigenous religious systems that have derived from Yorùbá which are often viewed as people who are unlikely to discuss their engagement in a formal counseling relationship. This study considered the nature of how many individuals who practice a religion based on Yorùbá traditions approach counseling from a different perspective. The philosophy of Carl Rogers’ person-centered theory was used as the framework for defining the counseling relationship and the counseling process. An …

Investigating The Influence Of Stress On Motivational Dysfunction In Male And Female Rodents, Emily Laine Errante

Graduate Research Theses & Dissertations

Motivational dysfunction is present in a variety of disorders, including major depressive disorder, schizophrenia, and Parkinsonism and can negatively impact social functioning, employment, and treatment response. Interestingly, outside stressors and stress hormones have been shown to contribute to depressive episodes in humans and depressive-like behaviors in rodents, including reducing motivated behaviors. Effort-related decision making procedures have been utilized in both humans and animals in order to examine motivational dysfunction, as it offers a subject a choice between high effort/reward and low effort/reward options, but little research has examined the effects of stress on effort-related decision making behaviors. Moreover, almost all …

Wetland Restoration Efforts Result In Increasing Phylogenetic Diversity, Nicholas T. Foster

Graduate Research Theses & Dissertations

Over the course of ecological restoration efforts, it has been observed that, despite restoration activities, species richness sometimes declines in a given habitat. While this response can be interpreted to mean that restoration activities are ineffective, other measures known as Phylogenetic Diversity Metrics can show that the community is actually becoming more diverse. Utilizing plant inventories collected as transect data from 1992-2021 of five wetland sites under various types of restoration in northern Illinois, a regional wetland community phylogeny was assembled. The community phylogeny was then analyzed for phylogenetic diversity measures through this 30-year period across the five sites. Additionally, …

Stress Analysis On Walls In Complex Fluids With Fluid-Solid Interaction, Dehong Fang

Graduate Research Theses & Dissertations

The wall shear stress (WSS) in blood vessels influences many biological processes, such as atherosclerosis, clot growth, angiogenesis, aneurysm rupture, and blood cell transport and adhesion. The deformable red blood cells (RBCs) in blood flow make the WSS even more complex. To analyze the WSS in complex fluids with cell suspensions, numerical simulations of blood flows in simple straight channels were conducted, where the fluid-solid interactions were simulated by immersed boundary-lattice Boltzmann method. The spatiotemporal dynamics of WSS were analyzed using dynamic mode decomposition (DMD), which is a technique that can extract the low-rank spatiotemporal features from complex fluids. The …

Understanding The Invasion History And Dispersal Patterns Of The Faucet Snail, Bithynia Tentaculata, Emma B. Grindle

Graduate Research Theses & Dissertations

Invasive species pose a major threat to biodiversity and are becoming increasingly common across the globe. The success of these invasive species often comes down to an ability to disperse effectively into novel environments over time and at multiple scales. Aquatic invasive species can be particularly challenging as they are not readily visible and are often well established before being formally observed. In the Upper Mississippi River region, an invasive European snail species, the faucet snail (Bithynia tentaculata) has invaded from Europe via the Great Lakes. The snail serves as an intermediate host for several species of trematode parasites that …

Factors Contributing To College Counselors’ Probability To Take A Client’S Sexual History, Adam Gregory

Graduate Research Theses & Dissertations

Many young adults are experiencing human sexuality related issues and disorders. Researchers have found ample evidence linking hindered human sexuality discussions with clients by mental health professionals to low and reduced comfort, lack of willingness, and non-positive attitudes about sex. There is limited research that shows there is a connection between sex-based discussions in the field of mental health and the lack of clinicians’ education and training in the field of human sexuality. One of the most essential ways to identify, treat, and promote sexual health is a thorough sexual history taking by clinicians. Mental health professionals agree that taking …

Transport Of Polyethylene Microplastic Spheres In A Sandy Aquifer, Alyssa Marie Graveline

Graduate Research Theses & Dissertations

Microplastic particles are currently listed as a Contaminant of Emerging Concern (CEC) since they are in much of Earth’s water systems and recent studies indicate plastic particles can sorb toxic contaminants. Microplastics (MP) are defined as plastic particles under 5 mm in length. Currently, there is a paucity of data on the transport of MP in the subsurface. This study investigated the transport, filtration, retardation, and influence of 5-100ߎm plastic polyethylene spheres in a shallow sandy aquifer over a distance of 1m by utilizing two forced hydraulic gradient tracer studies. Slug tests were used to estimate hydraulic conductivity prior and …

Through The Labyrinth: Lived Experiences Of Women And The Superintendency, Michelle Christine Graham

Graduate Research Theses & Dissertations

Men continue to dominate the superintendency in the United States, even though more than half of the specialized degrees needed to be a superintendent are earned by women (National Center for Education Statistics, 2019; Warner & Corley, 2017). Nationwide, 26.7% of the superintendents are women, while 27.75% of the superintendents in Illinois are women (Heffernan & Wasonga, 2017; Tienken, 2021). This study examined the lived experiences of five women superintendents from Illinois regarding their access to the superintendency and what they perceived to be significant factors along their trajectories. Data for this study was collected through one-on-one interviews using Seidman’s …

Estimating The Sex Of A Skeleton Using Measurements Of The Femur: Assessing Accuracy Among People Of Different Ancestry And Historic Periods, Hannah Decker

Graduate Research Theses & Dissertations

Two of the most common elements used for sex estimation in humans are the pelvis and the skull, as measurements of these two regions can provide highly accurate results. However, in some forensic cases and archaeological sites, the skull and pelvis may be damaged or missing. As a result, it is important to develop methods for estimating the sex of an individual from other bones of the body. In this study, I specifically examined the femur and determined classification accuracy rates for six femoral measurements: the supero-inferior neck diameter, epicondylar breadth, vertical head diameter, transverse femoral head diameter, femoral shaft …

Caregivers’ Inclination To See Their Children As Psychological Agents And Its Antecedents: A Cross-Cultural Study Of Parental Bonding, Reflective Functioning, And Maternal Mind-Mindedness, Chun-Hao Chiu

Graduate Research Theses & Dissertations

This dissertation examined difference in maternal mind-mindedness among American mothers, Taiwanese mothers, and mothers who emigrated from Taiwan and had their first child in the United States (Taiwanese Immigrant Mothers). It also investigated relationships between maternal mind-mindedness and mothers’ bonding with their parents during early years and their general mentalizing abilities for these three groups of mothers. One hundred twenty-three American mothers (Mage = 33.33 years old; SD = 5.31), 129 Taiwanese mothers (Mage = 35.93 years old; SD = 4.05), and 105 Taiwanese Immigrant Mothers (Mage = 36.85 years old; SD = 4.42) participated in this study online, through …

Perceptions Of Family-Of-Origin Belief Systems Among Cisgender Men In Outpatient Treatment For Sexual Offending: A Phenomenological Investigation, Jessie K. Huebner

Graduate Research Theses & Dissertations

Sexual offending is a public concern and results in lasting detrimental effects. To prevent sexual offending, it is important to identify potential factors that increase an individual’s vulnerability to engage in sexual offending behaviors. Researchers have identified a general connection between early life experiences, specifically family-of-origin experiences, and later sexual offending behaviors. There is also an established agreement on the transmission of thoughts and beliefs present within a family unit on the development of thoughts, beliefs, perceptions, and behaviors for a child as they age and into adulthood. Among individuals who offend sexually, implicit theories, the underlying beliefs that began …

Three Essays Examining The Impact Of Paid Maternity Leave Offers On Women’S Labor Market Outcomes, Murshed Jahan

Graduate Research Theses & Dissertations

This dissertation consists of three essays on the relationship between paid maternity leave benefit offers and different labor market outcomes of women of childbearing age. The first essay examines the impact of paid maternity leave offers in fringe benefit packages on wages of this particular group of women. Since the access to paid maternity leave and wage can be simultaneously determined by other factors, this essay uses an instrumental variable approach to estimate the effect. The information on access to paid maternity leave and other covariates for this analysis is extracted from the National Longitudinal Survey of Youth 1997 (NLSY97). …

Three Essays Examining The Effects Of Labor Market Conditions On College Enrollment And Completion, Dezhi Jiang

Graduate Research Theses & Dissertations

This research explores the causal effects of labor market conditions on college enrollment and attainment of high school graduates and answers the following questions: Does leaving high school in unfavorable labor market conditions cause a higher probability of college enrollment? Is this effect different between gender and across racial/ethnic groups? Does this effect vary regarding cognitive ability? Does this effect change over cohorts/generations? And does the positive effect on college enrollment in the short run translate into a positive effect on college degree attainment later?

The analysis that uses the National Longitudinal Survey of Youth 1997 (NLSY97) data suggests that …

A New Boundary Interlock Geometry Design Method For Fdm Multi-Material Part Strengthening, Shivaram Sree Venkata Shanmukha Kakaraparthi

Graduate Research Theses & Dissertations

Multi-material Fused Deposition Modelling (FDM) enables the cost-effective realization of designs in complex geometries built with multiple materials. However, achieving an adequate level of bonding strength between different materials remains a significant challenge. Besides the traditional way of addressing this challenge by strengthening material bonding at the molecular scale by optimizing the printing parameters, introducing a new design methodology that creates stronger multi-material interface geometry could also offer effective solutions. This work studied the mechanical properties of multi-material printed objects, with a particular focus on the interface zone formed between different materials at their geometrical boundaries. A new mathematical simulation …

Microbial Community And Soil Responses To Land Management Practices In Remnant And Restored Tallgrass Prairies, Desirae Marie Klimek

Graduate Research Theses & Dissertations

Critical ecosystem functions, such as decomposition and nutrient cycling, are driven by microbial communities within soil. As such, it is important to examine the effect of restoration practices, such as the presence of native grazers and prescribed burning, on these microbes and the soil they inhabit. The Nachusa Grasslands provides a chronosequence of restored tallgrass prairies ranging in restoration age from 5 to 33 years, as well as remnant prairies, and agricultural fields. These sites were sampled seasonally from 2013-2020 and microbial ribosomal RNA genes were surveyed to characterize soil microbial communities and assess how common restoration practices affect these …

Longitudinal Associations Between Parenting Stress, Adolescent Temperament, And Externalizing Behaviors, Kristina Kochanova

Graduate Research Theses & Dissertations

Although there is growing evidence that parenting stress and adolescent externalizing problems are linked cross-sectionally similarly to families of younger children, longitudinal research examining the direction of these associations among families of adolescents is limited. This study examined the direction of the link between parenting stress and adolescent externalizing problems over time and whether the associations differed due to adolescent temperament. Participants were 333 biological mothers (Mage = 40.15, SDage = 6.86; 75.7% White) with a 12- to 17-year-old adolescent. They were recruited through Amazon’s Mechanical Turk and completed psychometrically valid measures through Qualtrics at two time points, six months …

Impact Of Prairie Restoration On Geochemistry And Microbial Communities In Groundwater, Kayla Koenig

Graduate Research Theses & Dissertations

Prairie restoration is important for reversing the loss of biodiversity and repairing ecosystem functions humanity is dependent on diverse ecosystems. This study looks at the impact of the beginning phases of prairie restoration on groundwater geochemistry and microbial communities the relationship between them. This research project studied the geochemistry and microbial communities in five wells before, during, and after the beginning phases of the first year of a prairie restoration on the Northern Illinois University campus. Water samples were collected bimonthly for a year and analyzed on an Ion Chromatograph. Microbial samples were collected monthly and underwent DNA extraction, amplification, …

Aspergillus Nidulans Septa Are Indispensable For Surviving Cell Wall Stress, Ryland N. Spence, Walker Huso, Harley Edwards, Alexander Doan, Samantha Reese, Steven D. Harris, Ranjan Srivastava, Mark R. Marten

School of Biological Sciences: Faculty Publications

Septation in filamentous fungi is a normal part of development, which involves the formation of cross-hyphal bulkheads, typically containing pores, allowing cytoplasmic streaming between compartments. Based on previous findings regarding septa and cell wall stress, we hypothesized that septa are critical for survival during cell wall stress. To test this hypothesis, we used known Aspergillus nidulans septation-deficient mutants (ΔsepH, Δbud3, Δbud4, and Δrho4) and six antifungal compounds. Three of these compounds (micafungin, Congo red, and calcofluor white) are known cell wall stressors which activate the cell wall integrity signaling pathway (CWIS), while the three others (cycloheximide, miconazole, and 2,3-butanedione monoxime) …