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2022

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Evaluation Of Aluminum Dissolution, Current Density, And Pitting Patterns During Electrocoagulation, Monica Castro Carias Jun 2022

Evaluation Of Aluminum Dissolution, Current Density, And Pitting Patterns During Electrocoagulation, Monica Castro Carias

USF Tampa Graduate Theses and Dissertations

Electrocoagulation (EC) is a sustainable water treatment process involving the generation of coagulants on-site through the application of a voltage on metal (usually aluminum or iron alloys). The factors that impact EC system performance have been well researched, however further research is needed on the factors that impact anodic dissolution of aluminum electrodes, especially when the technology is transferred to full-scale applications. Electrode fouling is one of the factors that can impact dissolution of the anode during EC due to the formation of a protective oxide/hydroxide layer on the electrode surface. Previous studies have addressed electrode fouling by reversing polarity, …


Inhibitory Control Of Emotional Interference In Deaf Children: Evidence From Event-Related Potentials And Event-Related Spectral Perturbation Analysis, Qiong Chen, Junfeng Zhao, Huang Gu, Xiaoming Li Ph.D. Jun 2022

Inhibitory Control Of Emotional Interference In Deaf Children: Evidence From Event-Related Potentials And Event-Related Spectral Perturbation Analysis, Qiong Chen, Junfeng Zhao, Huang Gu, Xiaoming Li Ph.D.

Faculty Publications

Background: Impairment of interference control ability may reflect a more general deficit in executive functioning, and lead to an increase in internal-externalized problems such as impulsivity, which has been reported in deaf children. However, few researches have examined the neural mechanism of this impairment.

Methods: This study applied the electroencephalogram (EEG) technique to investigate the interference control ability in 31 deaf children and 28 hearing controls with emotional face-word stroop task.

Results: Results from behavioral task showed that deaf children exhibited lower accuracy compared to hearing controls. As for EEG analysis, reduced activation of ERP components in N1 and enhanced …


Crispr/Cas13 Effectors Have Differing Extents Of Off-Target Effects That Limit Their Utility In Eukaryotic Cells Jun 2022

Crispr/Cas13 Effectors Have Differing Extents Of Off-Target Effects That Limit Their Utility In Eukaryotic Cells

Faculty, Staff and Students Publications

CRISPR/Cas13 effectors have garnered increasing attention as easily customizable tools for detecting and depleting RNAs of interest. Near perfect complementarity between a target RNA and the Cas13-associated guide RNA is required for activation of Cas13 ribonuclease activity. Nonetheless, the specificity of Cas13 effectors in eukaryotic cells has been debated as the Cas13 nuclease domains can be exposed on the enzyme surface, providing the potential for promiscuous cleavage of nearby RNAs (so-called collateral damage). Here, using co-transfection assays in Drosophila and human cells, we found that the off-target effects of RxCas13d, a commonly used Cas13 effector, can be as strong as …


Top Tips For Clinical Governance In Primary Care, R Anderson, R Witton, E Mccoll Jun 2022

Top Tips For Clinical Governance In Primary Care, R Anderson, R Witton, E Mccoll

Peninsula Dental School

No abstract provided.


A New Species Of Neostenoptera Meunier (Diptera: Cecidomyiidae: Winnertziinae) From Hawai‘I, John D. Plakidas, Nhu H. Nguyen, Michael L. Ferro Jun 2022

A New Species Of Neostenoptera Meunier (Diptera: Cecidomyiidae: Winnertziinae) From Hawai‘I, John D. Plakidas, Nhu H. Nguyen, Michael L. Ferro

Insecta Mundi

A new paedogenetic midge (Diptera: Cecidomyiidae: Winnertziinae: Heteropezini) from O‘ahu Island, Hawai‘i, Neostenoptera hawaiiensis Plakidas, Nguyen, and Ferro, new species, is described and illus­trated. A key to all species in the genus is provided. Specimens were emergent from deadwood gathered at Waimea Arboretum and Botanical Garden. Neostenoptera appalachiensis Plakidas and Ferro were collected from the same set of samples in Hawai‘i, and additional specimens are reported from Georgia and South Carolina, three new state records. The discovery of two paedogenetic midges in Hawai‘i poses a unique set of questions as to their possible mode of arrival on an …


Tomoxia Bucephala A. Costa (Coleoptera: Mordellidae), A Palearctic Tumbling Flower Beetle Established In North America, R. F. C. Naczi, Robert A. Androw, John Rosenfeld Jun 2022

Tomoxia Bucephala A. Costa (Coleoptera: Mordellidae), A Palearctic Tumbling Flower Beetle Established In North America, R. F. C. Naczi, Robert A. Androw, John Rosenfeld

Insecta Mundi

Tomoxia bucephala A. Costa (Coleoptera: Mordellidae), a Palearctic tumbling flower beetle native to Europe, Asia, and northernmost Africa, is now known from North America. The first known occurrences were in 2015 in Essex and Union counties, New Jersey, U.S.A. and in 2019 in Passaic County, New Jersey, all in the New York City metropolitan area. An additional collection documents the species in 2016 from Allegheny County, Pennsylvania, in the Pittsburgh metropolitan area. The multiple occurrences, the large distance between those in New Jersey and Pennsylvania, and multiple detections in natural areas indicate T. bucephala is established in North America and …


Mentoring Undergraduate Research In Mathematical Modeling, Glenn Ledder Jun 2022

Mentoring Undergraduate Research In Mathematical Modeling, Glenn Ledder

Department of Mathematics: Faculty Publications

In writing about undergraduate research in mathematical modeling, I draw on my 31 years as a mathematics professor at the University of Nebraska–Lincoln, where I mentored students in honors’ theses, REU groups, and research done in a classroom setting, as well as my prior experience. I share my views on the differences between research at the undergraduate and professional levels, offer advice for undergraduate mentoring, provide suggestions for a variety of ways that students can disseminate their research, offer some thoughts on mathematical modeling and how to explain it to undergraduates, and discuss the challenges involved in broadening research participation …


Positive Rate-Dependent Action Potential Prolongation By Modulating Potassium Ion Channels, Candido Cabo Jun 2022

Positive Rate-Dependent Action Potential Prolongation By Modulating Potassium Ion Channels, Candido Cabo

Publications and Research

Pharmacological agents that prolong action potential duration (APD) to a larger extent at slow rates than at the fast excitation rates typical of ventricular tachycardia exhibit reverse rate dependence. Reverse rate dependence has been linked to the lack of efficacy of class III agents at preventing arrhythmias because the doses required to have an anti-arrhythmic effect at fast rates may have pro-arrhythmic effects at slow rates due to an excessive APD prolongation. In this report we show that, in computer models of the ventricular action potential, APD prolongation by accelerating phase 2 repolarization (by increasing IKs) and decelerating …


Multifunctional Nanomaterials For Energy Applications, Simas Rackauskas, Federico Cesano, Mohammed Jasim Uddin Jun 2022

Multifunctional Nanomaterials For Energy Applications, Simas Rackauskas, Federico Cesano, Mohammed Jasim Uddin

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)

No abstract provided.


Engineering The Lanthanide And Actinide Dopants Effects On The Local Environment Of Luminescent Complex Metal Oxide, Yuming Wang Jun 2022

Engineering The Lanthanide And Actinide Dopants Effects On The Local Environment Of Luminescent Complex Metal Oxide, Yuming Wang

LSU Doctoral Dissertations

Rare earth (RE) based phosphor materials are widely used as luminescent probes in high-temperature optical thermometry where standard methods are unsuitable. The current generation of luminescent thermometers lacks stability and energy transport within the operating temperature ranges. The challenge is developing an oxide-based material with high luminescence intensity and thermal sensitivity/stability, suitable for an optical temperature sensor/thermal barrier coating. This work aims to develop a fundamental understanding of the local environment surrounding RE or actinide dopants in metal oxide hosts to engineer their luminescence property. To maximize the luminescence, the local symmetry, doping concentration, and radiative/non-radiative relaxation pathways must be …


Efficacy And Outcomes Of Faecal Microbiota Transplantation For Recurrent Clostridioides Difficile Infection In Children With Inflammatory Bowel Disease, Zev Davidovics, Michael Docktor, Michael Dole, Jonathan M Gisser, Alka Goyal, Suchitra K Hourigan, M Kyle Jensen, Jess L Kaplan, Richard Kellermayer, Judith R Kelsen, Melissa A Kennedy, Sahil Khanna, Elizabeth D Knackstedt, Jennifer Lentine, Jeffery D Lewis, Sonia Michail, Paul D Mitchell, Maria Oliva-Hemker, Tiffany Patton, Karen Queliza, Sarah Sidhu, Aliza B Solomon, David L Suskind, Madison Weatherly, Steven Werlin, Edwin F De Zoeten, Stacy A Kahn, North American Society Of Pediatric Gastroenterology And Nutrition Faecal Microbiota Transplantation Special Interest Group Jun 2022

Efficacy And Outcomes Of Faecal Microbiota Transplantation For Recurrent Clostridioides Difficile Infection In Children With Inflammatory Bowel Disease, Zev Davidovics, Michael Docktor, Michael Dole, Jonathan M Gisser, Alka Goyal, Suchitra K Hourigan, M Kyle Jensen, Jess L Kaplan, Richard Kellermayer, Judith R Kelsen, Melissa A Kennedy, Sahil Khanna, Elizabeth D Knackstedt, Jennifer Lentine, Jeffery D Lewis, Sonia Michail, Paul D Mitchell, Maria Oliva-Hemker, Tiffany Patton, Karen Queliza, Sarah Sidhu, Aliza B Solomon, David L Suskind, Madison Weatherly, Steven Werlin, Edwin F De Zoeten, Stacy A Kahn, North American Society Of Pediatric Gastroenterology And Nutrition Faecal Microbiota Transplantation Special Interest Group

Faculty, Staff and Students Publications

BACKGROUND: Children with inflammatory bowel disease [IBD] are disproportionally affected by recurrent Clostridioides difficile infection [rCDI]. Although faecal microbiota transplantation [FMT] has been used with good efficacy in adults with IBD, little is known about outcomes associated with FMT in paediatric IBD.

METHODS: We performed a retrospective review of FMT at 20 paediatric centres in the USA from March 2012 to March 2020. Children with and without IBD were compared with determined differences in the efficacy of FMT for rCDI. In addition, children with IBD with and without a successful outcome were compared with determined predictors of success. Safety data …


Osteosarcoma Occurrence In Preclinical And Clinical Experiments With Teriparatide: A Qualitative Review, Wlla Wail Al-Halbouni, Moawia M. Al-Tabakha, Akram A. Ashames, Adi I. Arida, Muaed J. Alomar Jun 2022

Osteosarcoma Occurrence In Preclinical And Clinical Experiments With Teriparatide: A Qualitative Review, Wlla Wail Al-Halbouni, Moawia M. Al-Tabakha, Akram A. Ashames, Adi I. Arida, Muaed J. Alomar

The Thai Journal of Pharmaceutical Sciences

Teriparatide (TPTD) is one of the medications commonly prescribed for the treatment of osteoporosis since its approval in November 2002 by the U.S. Food and Drug Administration (FDA). While some preclinical toxicological studies have linked the drug to a dose-dependent increase in the occurrence of osteosarcoma, clinical trials showed the benefits outweighing the risk for TPTD-induced human osteosarcoma. The objective of this qualitative review was to qualitatively assess the literature for the association of osteosarcoma and the usage of TPTD in animal and human subjects. Studies were selected from all available records in the electronic databases covering the period from …


Developing A Scale To Measure Destination Gender, Tingting Zhang Jun 2022

Developing A Scale To Measure Destination Gender, Tingting Zhang

Rosen Research Review

Whether they depict the rugged landscape of the American West or the seductive charm of a South Asian beach, photographs and their accompanying text are powerful drivers of tourism demand. But what lies behind our response to such tourism tropes? Rosen College researchers have produced a new study which reappraises destination marketing through a gender-based lens. Led by Dr. Tingting Zhang, the study has developed and validated a cross-cultural scale to determine ‘destination gender’, or whether a location should be thought of as having masculine or feminine gender traits and appeal.


Effects Of Forest Restoration On The Recovery Of Dead Wood, Associated Arthropods, And Insect-Mediated Wood Decomposition, Estefania Fernandez Barrancos Jun 2022

Effects Of Forest Restoration On The Recovery Of Dead Wood, Associated Arthropods, And Insect-Mediated Wood Decomposition, Estefania Fernandez Barrancos

Dissertations

Dead wood represents 8% of terrestrial carbon stocks and is an important source of habitat and food for decomposer and non-decomposer arthropods. However, anthropogenic disturbance reduces the amount of dead wood, putting at risk the presence of a habitat that is essential for many arthropods and other organisms that rely on it for food and shelter. Forest restoration aims to assist the recovery of ecosystems that have been damaged or destroyed and could be a means to recover both dead wood and its associated arthropod communities. This doctoral dissertation lies at the intersection of climate change, biodiversity loss and ecological …


Medical School Watercooler Newsletter - June 23, 2022, Lindsey Lyle Jun 2022

Medical School Watercooler Newsletter - June 23, 2022, Lindsey Lyle

Watercooler Newsletter

This is the June 23, 2022 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents include:

  • Graduate students and faculty present at ATS International Conference
  • Meet a Med Student: Sarah Blackstock
  • USA Health residents, fellows compete in 2022 scholarship exposition


Effect Of Ischemia-Reperfusion Injury And Preconditioning On Lung Parenchyma After Acute Limb Ischemia, David Hutagaol, Dhama S. Susanti, Wuryantoro Soeharto, Muhammad A. Putra, Suprayitno Wardoyo, William Makdinata, Moira Setiawan Jun 2022

Effect Of Ischemia-Reperfusion Injury And Preconditioning On Lung Parenchyma After Acute Limb Ischemia, David Hutagaol, Dhama S. Susanti, Wuryantoro Soeharto, Muhammad A. Putra, Suprayitno Wardoyo, William Makdinata, Moira Setiawan

The New Ropanasuri Journal of Surgery

Introduction. Acute limb ischemia, a sudden decrease of perfusion to the extremities, can compromise the survival of the limbs. Medical intervention and surgery are often needed to return perfusion. However, reperfusion injury can trigger oxidative stress and inflammatory response, leading to local and remote tissue damage, such as the lungs, which increases morbidity and mortality. This research aims to study the effects of hypothermia and remote ischemic preconditioning (RIPC) on the lung parenchyma after being exposed to reperfusion after acute limb ischemia in rabbits.

Method. Eighteen New Zealand White rabbits were divided into three groups of 6. The …


Nitrogen Fixation Rates In Forested Mountain Streams: Are Sediment Microbes More Important Than Previously Thought?, Erica A. H. Bakker, Carmella Vizza, Clay Arango, Sarah S. Roley Jun 2022

Nitrogen Fixation Rates In Forested Mountain Streams: Are Sediment Microbes More Important Than Previously Thought?, Erica A. H. Bakker, Carmella Vizza, Clay Arango, Sarah S. Roley

Biology Faculty Scholarship

  1. Biological nitrogen (N) fixation, the microbial conversion of N2 gas to ammonia, makes N available to food webs. Low-N streams often have a high relative abundance of N-fixing taxa, suggesting that N fixation is an important N source in these systems. Despite this potential, stream N fixation has not been well-characterised, particularly compared to lakes and marine environments. One unknown is the relative contributions of various N-fixing organisms, particularly heterotrophic microbes.
  2. In low-N streams in the Cascade Mountains (Washington, USA), three groups of N-fixers predominate: cyanobacteria (Nostoc paramelioides) colonies that house a midge symbiont (Cricotopus spp.), …


Peripherally Restricted Transthyretin-Based Delivery System For Probes And Therapeutics Avoiding Opioid-Related Side Effects, Md Tariqul Haque Tuhin, Dengpan Liang, Fang Liu, Hala Aldawod, Toufiq Ul Amin, Joshua S. Ho, Rasha Emara, Arjun D. Patel, Melanie A. Felmlee, Miki S. Park, James A. Uchizono, Mamoun M. Alhamadsheh Jun 2022

Peripherally Restricted Transthyretin-Based Delivery System For Probes And Therapeutics Avoiding Opioid-Related Side Effects, Md Tariqul Haque Tuhin, Dengpan Liang, Fang Liu, Hala Aldawod, Toufiq Ul Amin, Joshua S. Ho, Rasha Emara, Arjun D. Patel, Melanie A. Felmlee, Miki S. Park, James A. Uchizono, Mamoun M. Alhamadsheh

School of Pharmacy Faculty Articles

Several investigations into the sites of action of opioid analgesics have utilized peripherally acting mu-opioid receptor antagonists (PAMORAs), which have been incorrectly assumed to possess limited permeability across the blood-brain barrier. Unfortunately, the poor pharmacokinetic properties of current PAMORAs have resulted in misunderstandings of the role of central nervous system and gastrointestinal tract in precipitating side effects such as opioid-induced constipation. Here, we develop a drug delivery approach for restricting the passage of small molecules across the blood-brain barrier. This allows us to develop naloxone- and oxycodone-based conjugates that display superior potency, peripheral selectivity, pharmacokinetics, and efficacy in rats compared …


Experience Life In A Refugee Camp - And Learn How To Help - June 25, Staff Jun 2022

Experience Life In A Refugee Camp - And Learn How To Help - June 25, Staff

Browse All News

No abstract provided.


Data As A Service Ecosystem For Data-Driven Research, Leonardo Vieira Jun 2022

Data As A Service Ecosystem For Data-Driven Research, Leonardo Vieira

Theses and Dissertations

For the last two decades, the cloud computing ecosystems has become a major defining force for America, because of their unique economic, social and national importance. These ecosystems have now taken their place alongside the nation’s other infrastructure such as the food/agricultural, energy healthcare, and roads/highways. Scientists, engineers, and researchers over the same period have experienced a tremendous growth in the need for resources to that support assorted research. Cloud computing enables these communities to undertake wide-ranging research efforts, while requiring no maintenance, management or significant invest of local resources. The genius of this project examines how create on-ramps for …


Butte Priority Soils Operable Unit (Bpsou) Draft Final Insufficiently Reclaimed Sites - Field Sampling Plan (Fsp) Bres No. 93 – Soudan Dump., Mike Mcanulty Jun 2022

Butte Priority Soils Operable Unit (Bpsou) Draft Final Insufficiently Reclaimed Sites - Field Sampling Plan (Fsp) Bres No. 93 – Soudan Dump., Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.


Butte Priority Soils Operable Unit (Bpsou) Draft Final Insufficiently Reclaimed Sites - Field Sampling Plan (Fsp) Bres No. 96 – Washoe Dump, Mike Mcanulty Jun 2022

Butte Priority Soils Operable Unit (Bpsou) Draft Final Insufficiently Reclaimed Sites - Field Sampling Plan (Fsp) Bres No. 96 – Washoe Dump, Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.


Reverse-Coded Items Do Not Work In Spanish: Data From Four Samples Using Established Measures, Amanda Venta, Cassandra A. Bailey, Jesse Walker, Alfonso Mercado, Cecilia Colunga-Rodríguez, Mario Ángel-González, Gabriel Davalos-Picazo Jun 2022

Reverse-Coded Items Do Not Work In Spanish: Data From Four Samples Using Established Measures, Amanda Venta, Cassandra A. Bailey, Jesse Walker, Alfonso Mercado, Cecilia Colunga-Rodríguez, Mario Ángel-González, Gabriel Davalos-Picazo

Psychological Science Faculty Publications

The potential for suboptimal psychometric performance of reverse-coded items may be particularly pronounced when scales are translated and administered in Spanish with these problems exacerbated in youth respondents. This is a significant concern, given the rapid rise in Hispanic-American and Spanish-speaking individuals in the US and their rightful, growing representation in psychological research and clinical care. The aim of this study was to examine the psychometric performance of reverse-coded items across four Spanish-speaking samples spanning developmental stages including youth, college students, and parents (N = 1,084; Adolescents n = 107; M = 19.79; SD = 2.09; 41.1% female; Caregivers …


Improved Performance Of Silica-Supported La0.5Ba0.5Feo3 In The Reverse Water Gas Shift - Chemical Looping Process For Carbon Dioxide Reduction – A Density Functional Theory Study, Jiawei Guo Jun 2022

Improved Performance Of Silica-Supported La0.5Ba0.5Feo3 In The Reverse Water Gas Shift - Chemical Looping Process For Carbon Dioxide Reduction – A Density Functional Theory Study, Jiawei Guo

USF Tampa Graduate Theses and Dissertations

Global warming is increasingly obvious, and the reduction of greenhouse gases is an effective way to heal. Increasing the efficiency of catalysts that is applied in the industry can significantly reduce the emission of greenhouse gases. Reverse water gas shift chemical looping (RWGS-CL) is a promising reaction to convert CO2 to CO. La0.5Ba0.5FeO3 (LBF) is a good candidate for RWGS-CL, which shows increased conversion yield when supported on silica. This research focuses on identifying the mechanism of RWGS-CL via silica-supported LBF by exploring the oxygen vacancy formation energy (EO-vac). Density Functional Theory (DFT) is a powerful computational method to solve …


University Officials Mark High Point In New Stem Facility, Edwin B. Smith Jun 2022

University Officials Mark High Point In New Stem Facility, Edwin B. Smith

University of Mississippi News

Ceremony marks topping out of the Center for Science and Technology Innovation


My Authentic Self: Rousseau And The Ethics Of Authenticity In The Age Of Gender Ideology, Steven Kessler Jun 2022

My Authentic Self: Rousseau And The Ethics Of Authenticity In The Age Of Gender Ideology, Steven Kessler

Journal of Ideology

This article examines the origins of the contemporary iteration of gender ideology pervasive in the western world today. To accomplish this, the article is broken into three main components. The first component examines the source of where the current trend in picking pronouns, genders, and sexual orientations emanates from. This is attributed to an ideology called, “the ethics of authenticity.” The tenets of the ethics of authenticity are delineated in the article. Another component of the article attributes the origin of authenticity to the work and thought of Jean Rousseau. Specifically, four of his works are examined as fundamentally important …


We Can Update Our Own Stories: Trans*-Informed Principles For Gender-Inclusive Science Teaching., K. Ren Rende Jun 2022

We Can Update Our Own Stories: Trans*-Informed Principles For Gender-Inclusive Science Teaching., K. Ren Rende

Teacher Education Faculty Publications

Across the United States, Anti-LGBTQ curriculum laws are being passed in staggering numbers, impacting an estimated 25 million children. At a time where a majority of LBGTQ students report feeling unsafe at school, a significant education challenge is how to meet the needs of these students while addressing and dismantling the way the institution of science education continues to uphold systems of oppression. While there have been waves of LGBTQinclusive science education reform, these movements have been stymied by a lack of cohesive guidelines for practice and are generally limited in their inclusion of considerations that attend to the needs …


Wmu Board Of Trustees Formal Session June 23, 2022, Wmu Board Of Trustees Jun 2022

Wmu Board Of Trustees Formal Session June 23, 2022, Wmu Board Of Trustees

WMU Board of Trustees Meetings

Acceptance of the Agenda

Approval of the Minutes

Remarks by the Chair

Remarks by the President

Updates by the Faculty Senate President

Updates by the Western Student Association President

Updates by the Graduate Student Association Vice President

Presentation – WMU’s Investment in the Future – Academic Tenure and Promotion

Public Comments Regarding Action Items

Action Items

  • Proclamation - Celebration of the 50th Anniversary of Title IX
  • Academic Tenure and Promotion
  • Teaching Assistants Union (TAU) Labor Agreement
  • Police Officers Association (POA) Labor Agreement
  • Tuition and Required Fee Rates Fall 2022
  • General Fund Budget, Fiscal Year 2022-2023
  • Student Room, Board, and Apartment …


Lahontan Cutthroat Trout (Oncorhynchus Clarkii Henshawi) And Paiute Cutthroat Trout (Oncorhynchus Clarkii Seleniris) Detection From Environmental Dna Samples: A Dual-Purpose Assay, Thomas W. Franklin, Joseph C. Dysthe, Helen Neville, Michael K. Young, Kevin M. Mckelvey, Michael K. Schwartz Jun 2022

Lahontan Cutthroat Trout (Oncorhynchus Clarkii Henshawi) And Paiute Cutthroat Trout (Oncorhynchus Clarkii Seleniris) Detection From Environmental Dna Samples: A Dual-Purpose Assay, Thomas W. Franklin, Joseph C. Dysthe, Helen Neville, Michael K. Young, Kevin M. Mckelvey, Michael K. Schwartz

Western North American Naturalist

Lahontan cutthroat trout (Oncorhynchus clarkii henshawi) and Paiute cutthroat trout (Oncorhynchus clarkii seleniris) are rare taxa of conservation concern. Their generally low population densities and remote distribution make traditional sampling difficult, but they primarily exist in small streams, a habitat type where environmental DNA (eDNA) sampling has proven extremely effective. We developed a single eDNA assay that allows sensitive detection of both taxa, which occur separate from each other in different regions of the Lahontan Basin. Performance of the assay was evaluated using most lineages of Lahontan cutthroat trout and Paiute cutthroat trout as well as …


A Mathematical Model Of Glut1 Modulation In Rods And Rpe And Its Differential Impact In Cell Metabolism, Andrea Aparicio, Erika T Camacho, Nancy J. Philp, Stephen A Wirkus Jun 2022

A Mathematical Model Of Glut1 Modulation In Rods And Rpe And Its Differential Impact In Cell Metabolism, Andrea Aparicio, Erika T Camacho, Nancy J. Philp, Stephen A Wirkus

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

We present a mathematical model of key glucose metabolic pathways in two cells of the human retina: the rods and the retinal pigmented epithelium (RPE). Computational simulations of glucose transporter 1 (GLUT1) inhibition in the model accurately reproduce experimental data from conditional knockout mice and reveal that modification of GLUT1 expression levels of both cells differentially impacts their metabolism. We hypothesize that, under glucose scarcity, the RPE’s energy producing pathways are altered in order to preserve its functionality, impacting the photoreceptors’ outer segment renewal. On the other hand, when glucose is limited in the rods, aerobic glycolysis is preserved, which …