Digital Commons Network^™

Edible Mushrooms: An Emerging Therapeutic Of Diabetes, Nilanjan Chakraborty, Anuron Banerjee, Sudeshna Nandi, Swagata Sarkar, Krishnendu Acharya

The Thai Journal of Pharmaceutical Sciences

Diabetes is the third most customary non-infectious disease occurring worldwide and leads to untimely disability and mortality. This metabolic disorder is characterized by impaired insulin secretions and high blood glucose levels. Among others, type 2 diabetes is the most common. Various chemical and biochemical hypoglycemic drugs are utilized to control and treat hyperglycemia but required further interventions to reduce their toxicity and side effects on the users. Edible mushrooms have been employed from ancient times to heal many diseases as it composed of anti-oxidant, alkaloids, fibers, triterpenoids, and other phytochemicals. The bioactive secondary metabolites of mushrooms can improve insulin resistance …

Eluication Of Lipid Metabolic Pathways In Differentiating Giardia Lamblia Using High Resolution Mass Spectrometry, Cameron Ellis

Open Access Theses & Dissertations

Giardia lamblia is an intestinal protozoan found worldwide, including the U.S. This parasite exists in two morphologic stages - a replicative trophozoite and a relatively dormant yet viable cyst. While exposures of cysts to gastric acid during passage through the human stomach induces excystation, factors in the small intestine, where trophozoites colonize trigger encystation or cyst formation. Transformation into cyst stage is essential for Giardia to survive in the environment for months before infecting new hosts. Because of its small genome size (11.7 Mb), metabolic pathways in Giardia are highly reduced. As far as lipid metabolism is concerned, only limited …

Development Of A Ph-Triggered Cargo Delivery System With Esxa Mutants, Shuchen Chen

Open Access Theses & Dissertations

Tuberculosis (TB) is a communicable disease caused by Mycobacterium tuberculosis (Mtb). It was the leading cause of human death generated by a single infectious agent before the Coronavirus (COVID-19) pandemic. Mtb has a special mechanism to evade the hostâ??s immune system and utilize macrophages as reservoirs. Latent TB infections have been a threat to public health. Liposome has been widely used for drug delivery. Immunoliposomes are special liposome carriers coated with ligands, such as antigens, targeting certain cells or organs to regulate the uptake of liposome. By constructing the liposome components, or surface modification, cargo release of liposome can be …

Characterization Of Esxa/Esxb Heterodimer Membrane Interaction With A Novel Popc/Popg Liposome Model, Salvador Vazquez Vazquez Reyes

Open Access Theses & Dissertations

EsxA and EsxB are co-secreted as a heterodimer and have been shown to play critical roles in phagosome rupture and translocation of Mycobacterium tuberculosis into the cytosol. Current in vitro studies support a model in which the EsxA:EsxB heterodimer is dissociated upon acidification, which might allow EsxA insertion into liposome membranes. While the membrane permeabilizing activity (MPA) of EsxA has been well characterized in liposomes composed of di-oleoyl-phosphatidylcholine (DOPC), the MPA of EsxA:EsxB heterodimer has not been detected with the DOPC liposome. In this study, we established a new in vitro membrane assay to test the MPA of EsxA and …

Reflections On The Adaptation Of A Postgraduate Degree In Water Management From In-Person To Remote Delivery, Murray Clamen, Emma Anderson, Johanna Dipple, Jan Adamowski

Journal of Contemporary Water Research and Education

In early 2020, the COVID-19 pandemic spurred the rapid adaptation of university course delivery to an online format. Though in-person delivery partially resumed in the Fall of 2021, future conditions may favor a return to, or addition of, remote delivery. It is therefore important for instructors, program directors, and institutions to capitalize on this learning opportunity and reflect on adaptation measures’ successes (and failures) to inform future online course design. The reworking of McGill University's Master of Science Program in Integrated Water Resources Management (IWRM) provides a case study to evaluate the adaptation of remote teaching of water resource management. …

Developing Confidence And Interest In Teaching Relevant Mathematical Modeling Lessons, Jacy Beck

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

What is mathematical modeling and how can inservice and pre-service teachers develop the skills and competencies necessary to increase confidence and interest in teaching relevant mathematical modeling lessons? Mathematical modeling is “the process of choosing and using appropriate mathematics and statistics to analyze empirical situations, to understand them better, and to improve decisions” (CSSM, 2010, p. 72). By providing students with an opportunity to engage in relevant mathematical modeling prompts, we provide them with transferable skills and knowledge. The aim of this paper will be to provide insight into the relevance of teaching mathematical modeling, provide resources for integrating modeling …

Modeling A Pandemic: Investigating Student Learning About Disease Spread In The Context Of Agent-Based Modeling, Siyu Wu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The COVID-19 pandemic has highlighted a need for students to learn about public health issues, including the transmission of disease and methods for the prevention of epidemics. This study presents data from a project focused on developing computational microworlds to help middle school students learn about these topics. The microworld is designed to help students model and test their ideas about how a disease spreads through a population and how an epidemic can be prevented. I employed a lab-based case study approach to conduct one-on-one 1.5-hour interviews through Zoom with four middle-school students (ages 12-14). During the interview, the student …

Pesticide-Leaf Interactions And Their Implications For Pesticide Fate Modeling, Ashlie Kinross

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The work described here provides measured data to improve the understanding of the interaction between a pesticide and leaf surface after application. Two methods were developed, one using a newly introduced instrument, for the extraction of pesticides from leaves. This is required to measure the concentration of pesticide in the leaves. Using one of the developed extraction methods, measurements were made to determine how a pesticide equilibrates between a leaf and the air above it. These measurements were incorporated into a pesticide fate model that predicts how a pesticide moves through the environment after application to an agricultural field. The …

Chondronecrosis With Osteomyelitis In Broilers: Bacterial Species Involvement And Influence Of Probiotics And Synbiotics, Amer Abdullah Hasan

Graduate Theses and Dissertations

Since 1945, the market for broiler production has evolved from a collection of smallholder poultry farms into an industry worth billions of dollars. Poultry production has not gone without challenges. Lameness in young broilers is presently a serious issue in the poultry industry for reasons of animal health and welfare, as well as significant lost production and revenue. Bacterial chondronecrosis with osteomyelitis (BCO) is a major form of lameness in broilers. This dissertation contains information about (i) the influence of Staphylococcus agnetis, Staphylococcus saprophyticus, Staphylococcus epidermidis and Enterococcus faecalis on inducing BCO lameness, (ii) evaluation of of probiotics for reducing …

Examination Of Amphibian Community And Environmental Relationships In South Texas Using Environmental Dna (Edna), Sean Michael Collins

Theses and Dissertations

Developing more efficient tools to assess amphibian biodiversity and understanding what environmental variables drive amphibian biodiversity are top priorities, as amphibians are facing extinction events across the globe. Environmental DNA (eDNA) surveys are a promising new tool to asses amphibian biodiversity. Throughout the study eDNA metabarcoding along with a targeted eDNA assay and traditional survey methods were used to provide foundational information on amphibian community assemblages throughout South Texas. Water quality, habitat characteristics and soil composition data were collected and used to examine environmental relationships. eDNA metabarcoding detected significantly more amphibian taxonomic units compared to traditional survey …

Evaluation Of The Feeding Value Of Proso Millet In Growing-Finishing Diets For Pigs And Effects Of Feed Ingredients And Medium-Chain Fatty Acids On Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Survivability, Khang Nguyen

Department of Animal Science: Dissertations, Theses, and Student Research

The objective of the project were to: 1) evaluate the feeding value of proso millet as a potential substitute for corn in corn-soybean meal-based diets for pigs; and 2) investigate the relationship between feed ingredients and virus survivability, followed by examining feed additive candidates that could mitigate the risk of transmission of viral pathogens.

In Exp. 1, 36 barrows were fed 1 of 4 dietary treatments, including: Diet 1, corn-soybean meal-based (control), and Diets 2, 3, and 4 had proso millet replacing 33%, 67%, and 100% of corn in the control diet, respectively. During Phase 3, pigs consuming the proso …

Investigating Bacterial Community Composition And Antimicrobial Resistance Genes In Beef Cattle, Arena E. See

Department of Animal Science: Dissertations, Theses, and Student Research

While antibiotics have been critical over the last decade for curbing infectious diseases, they have contributed to the rise of antimicrobial resistance (AMR). Infections resulting from antimicrobial-resistant pathogens are correlated with severe morbidity, mortality, and healthcare costs. With the increasing concerns regarding AMR and its emergence, it is paramount to understand not only the effects of antibiotics on microbial community and diversity, but also to develop a novel tool that gives us a better understanding of the spread and functional traits of AMR genes.

To this end, rumen, fecal, and pen surface samples were collected from 72 animals at United …

Covid-19 Vaccine Hesitancy Among Non-Refugees And Refugees In Kenya, Ryan Rego, Anthony Ngugi, Antonia Johanna Sophie Delius, Stanley Luchters, Joseph C. Kolars, Furqan B. Irfan, Eileen Weinheimer-Haus, Amina Abubakar, Reena Shah, Ji Zhu

Population Health, East Africa

Factors associated with COVID-19 vaccine hesitancy (which we define as refusal to be vaccinated when asked, resulting in delayed or non- vaccination) are poorly studied in sub-Saharan Africa and among refugees, particularly in Kenya. Using survey data from wave five (March to June 2021) of the Kenya Rapid Response Phone Survey (RRPS), a household survey representative of the population of Kenya, we estimated the self-reported rates and factors associated with vaccine hesitancy among non-refugees and refugees in Kenya. Non-refugee households were recruited through sampling of the 2015/16 Kenya Household Budget Survey and random digit dialing. Refugee households were recruited through …

Pulmonary Fibrosis Model Using Micro-Ct Analyzable Human Psc-Derived Alveolar Organoids Containing Alveolar Macrophage-Like Cells, Seri Choi, Jiwoong Choi, Seongmin Cheon, Jihong Song, Seung-Yeon Kim, Ji-Eun Kim, Dae-Hwan Nam, Gohar Manzar, Su-Man Kim, Hyung-Sik Kang, Kee K Kim, Sang Hoon Jeong, Ju-Han Lee, Eun-Kee Park, Minseob Lee, Hyang-Ae Lee, Ki-Suk Kim, Han-Jin Park, Won Keun Oh, Chungoo Park, Chang Hyun Lee, Eun-Mi Kim

Faculty, Staff and Student Publications

Human lung organoids (hLOs) are useful for disease modelling and drug screening. However, a lack of immune cells in hLOs limits the recapitulation of in vivo cellular physiology. Here, we generated hLOs containing alveolar macrophage (AMφ)-like cells derived from pluripotent stem cells (PSC). To bridge hLOs with advanced human lung high-resolution X-ray computed tomography (CT), we acquired quantitative micro-CT images. Three hLO types were observed during differentiation. Among them, alveolar hLOs highly expressed not only lung epithelial cell markers but also AMφ-specific markers. Furthermore, CD68

Functional Characterization Of The Toxoplasma Gondii Endolysosomal System, Leah Brock Thornton

All Dissertations

Toxoplasma gondii is an obligate intracellular parasite that can infect a wide range of mammalian hosts. T. gondii can cause severe disease in immunocompromised individuals as well as pregnant women, in which congenital toxoplasmosis can lead to spontaneous abortion and fetal blindness. T. gondii infects up to roughly one-third of the global human population and is the causative agent of toxoplasmosis. Given this health burden of toxoplasmosis and that antibiotics currently used for the treatment of infection lead to strong side effects, identification of parasite-specific targets are necessary for novel therapeutic strategies.

T. gondii parasites contain a dynamic and active …

Immunotherapeutic Effects Of Intratumorally Injected Zymosan-Adenovirus Conjugates Encoding Constant Active Irfs In A Melanoma Mouse Model, Margaret Musick

All Dissertations

Current immunotherapies are only effective in a fraction of cancer patients. One of the main contributing factors to therapy resistance and overall poor prognosis is the immune status of the tumor microenvironment (TME). Tumor-associated macrophages (TAMs) have been shown to have significant influence on the immune status of the TME. As key players in maintaining tissue homeostasis, macrophages exhibit a broad range of phenotypes and functions, making TAMs a promising target for immunomodulatory therapies. As liaisons between innate and adaptive immunity, TAMs can promote a robust anti-tumor response involving both innate and adaptive immunity. This study investigated the potential of …

Characterization Of Kinases And Hypothetical Proteins In The Entamoeba Species, Heather Andrews Walters

All Dissertations

Entamoeba histolytica is a protozoan parasite that causes amebic dysentery and amoebic liver abscess. This pathogen possesses a two-stage life cycle consisting of an environmentally stable latent cyst and a pathogenic amoeboid trophozoite. Since infection is acquired by ingestion of cysts from contaminated food and water, this parasite is prevalent in underdeveloped countries. A reptilian pathogen, Entamoeba invadens, which can encyst in culture, has long-served as a surrogate to study stage conversion. Much remains unclear about stage conversion and the stress response in these parasites and current treatments for amoebiasis are lacking, as they cause severe side effects. Ultimately …

The Association Of Prothrombin Gene Mutations And Cytomegalovirus Infection With Abortion Among Iraqi Women, Eman F Turkey, Abbas G Hamad, Abbas K Al-Mansoori

Baghdad Science Journal

Abortion is categorized as the termination of conception caused by the failure or removal of the embryo from the uterus before the conclusion of pregnancy. Microorganisms and genetic factors are two of the many factors associated with abortion. Cytomegalovirus is a widespread congenital virus infection pathogen that affects a wide variety of people. The prothrombin gene is one of the essential causes that trigger blood clotting and the function of abortion women, therefore the aim of the study is to detect and associate Cytomegalovirus and prothrombin gene mutation (Gene ID: 14061 in NCBI) with abortion through genetic and immunological methods. …

The Intricate Interplay Between Cancer Stem Cells And Cell-Of-Origin Of Cancer: Implications For Therapeutic Strategies, Luis Alberto Vega, Misu A Sanson, María Belén Cubria, Shrijana Regmi, Brittany J Shah, Samuel A Shelburne, Anthony R Flores

Faculty, Staff and Student Publications

Antimicrobial resistance-encoding mobile genetic elements (MGEs) may contribute to the disease potential of bacterial pathogens. We previously described the association of Group A Streptococcus (GAS) derived from invasive disease with increasingly frequent antimicrobial resistance (AMR). We hypothesized that a 65-kb AMR-encoding MGE (ICESpyM92), highly conserved among closely related emergent invasive emm92 GAS, contributes to GAS disease potential. Here, we provide evidence that a combination of ICESpyM92- and core genome-dependent differential gene expression (DGE) contributes to invasive disease phenotypes of emergent emm92 GAS. Using isogenic ICESpyM92 mutants generated in distinct emm92 genomic backgrounds, we determined the presence of ICESpyM92 enhances GAS …

Acylcarnitines And Genetic Variation In Fat Oxidation Genes In Hiv-Infected, Antiretroviral-Treated Children With And Without Myopathy, Brian Kirmse, Charlotte Hobbs, Lisa Aaron, Grace Montepiedra, Marshall Summar, Paige L Williams, Caitlin J Smith, Russell Van Dyke, Chunli Yu, Kelli K Ryckman, William Borkowsky

Faculty, Staff and Student Publications

BACKGROUND: Mitochondrial toxicity resulting in myopathy and lactic acidosis has been described in antiretroviral (ARV)-exposed patients. We hypothesized that myopathy in HIV-infected, ARV-treated children would be associated with metabolic (acylcarnitines) and genetic (variants in metabolic genes) markers of dysfunctional fatty acid oxidation (FAO).

METHODS: Acylcarnitine profiles (ACP) were analyzed for 74 HIV-infected children on nucleoside reverse transcriptase inhibitor (NRTI)-containing ARV. Thirty-seven participants with ≥2 creatine kinase measurements >500 IU (n = 18) or evidence of echocardiographic cardiomyopathy (n = 19) were matched with 37 participants without myopathy. Single nucleotide polymorphisms (SNPs) in FAO genes were also evaluated.

RESULTS: Abnormal ACP …

Antibody Evasion By Sars-Cov-2 Omicron Subvariants Ba2121, Ba4 And Ba5, Qian Wang, Yicheng Guo, Sho Iketani, Manoj S Nair, Zhiteng Li, Hiroshi Mohri, Maple Wang, Jian Yu, Anthony D Bowen, Jennifer Y Chang, Jayesh G Shah, Nadia Nguyen, Zhiwei Chen, Kathrine Meyers, Michael T Yin, Magdalena E Sobieszczyk, Zizhang Sheng, Yaoxing Huang, Lihong Liu, David D Ho

Faculty, Staff and Student Publications

SARS-CoV-2 Omicron subvariants BA.2.12.1 and BA.4/5 have surged notably to become dominant in the United States and South Africa, respectively1,2. These new subvariants carrying further mutations in their spike proteins raise concerns that they may further evade neutralizing antibodies, thereby further compromising the efficacy of COVID-19 vaccines and therapeutic monoclonals. We now report findings from a systematic antigenic analysis of these surging Omicron subvariants. BA.2.12.1 is only modestly (1.8-fold) more resistant to sera from vaccinated and boosted individuals than BA.2. However, BA.4/5 is substantially (4.2-fold) more resistant and thus more likely to lead to vaccine breakthrough infections. Mutation at spike …

Performance Evaluation Of The Xt Microslide Assay Pairs On The Vitros Xt 7600 Compared To Vitros Single Microslide Assays On Vitros 5600, Lily Olayinka, Estella Tam, Sridevi Devaraj

Faculty, Staff and Students Publications

OBJECTIVES: Pediatric hospitals are always challenged by specimen volumes and thus any innovation in this realm is very welcome. With the introduction of Microslide assay pairs, we aimed to evaluate the analytical performance of the Vitros XT MicroSlide assay pairs on the Vitros XT 7600 compared to single MicroSlides.

DESIGN: Performance characteristics included within-run precision, analytical measurable range, method comparison, and interference verification. We compared six XT MicroSlide pairs on the Vitros XT 7600 with twelve corresponding single slide assays on the Vitros 5600 system.

RESULTS: The XT MicroSlides on Vitros XT 7600 demonstrated excellent precision, equivalent analytical measurable range, …

Microplastics In Freshly Fallen Snow: How May It Adversely Impact Human Health And Exacerbate The Covid-19 Crisis?, Ayesha Liaquat, Aleena Kashif, Sushma Rathi, Alishba Raza

Medical College Documents

No abstract provided.

Covid-19 And Hospitality Services: The Role Of Information Sources, Believability, Fear, And Behavioral Intentions, Shaniel Bernard, Imran Rahman, Sijun Liu, Luana Nanu

Journal of Global Hospitality and Tourism

Based on the Social Amplification of Risk Framework (SARF) and crisis decision theory, this study examined the influence of trust in different types of information sources on the believability of COVID-19 information (BCI). Furthermore, the influence of BCI on fearfulness and the corresponding influence of fearfulness on the intention to use hospitality services and stay at home are analyzed. Structural equations modeling, using data from 1,017 American consumers, successfully confirmed the significant influences of trust in media and government on BCI and the corresponding positive effect of BCI on fearfulness. Additionally, the negative effects of fearfulness on intentions to visit …

Mathematical Modeling Of Seir Model With Generalized Incidence Function And The Extension To Covid-19 Model, Shymaa Mohammad Dadoa

Theses

The COVID-19 pandemic had shown the importance of the SEIR model in predicting the outcome of the disease spread and to find the best strategies to contain the pandemic. As this type of model has a limited number of compartments, many other models were derived from the SEIR model to cover, to the maximum, the complex dynamics of the disease spread. These extensions of the SEIR model bring natural validity questions: How can we validate these models? and how far/close are these extended models from giving us real insights into the pandemic?

This thesis investigates the SEIR epidemic model and …

Investigating The Biorisk Of Genetically Engineered Thermosynechococcus Elongatus Bp1, Cherrelle Leah Barnes

Chemistry & Biochemistry Theses & Dissertations

Cyanobacteria, also known as blue-green algae, are an ancient group of microorganisms that use simple materials, such as sunlight, carbon dioxide and water, to produce energy while providing oxygen to the atmosphere by performing photosynthesis. Synthetic biology approaches have been employed with cyanobacteria as a platform to produce a range of products, such as biofuels, by inserting a series of genes into the cyanobacterial genome that will allow the conversion of metabolic intermediates to such desired products. Although these methods are promising, it is important to understand any potential bio-risk they pose. This research evaluates the potential bio-risk of genetically …

Cyberchondria And Covid-19 Health Threats Related To Attentional Bias And Safety Behaviors, Jacqueline J. Charles

Electronic Theses and Dissertations

Cyberchondria is described as excessively searching online for health information that ultimately increases worry and anxiety about one’s health (Starcevic et al., 2019). Research has demonstrated an increased attentional bias in anxious individuals for threat-related stimuli. Attentional bias for health information exacerbated by the frequent exposure to health threats during the COVID-19 pandemic may contribute to additional health-seeking behavior. The current study aimed to explore the potential relationship between COVID-19 health threats and cyberchondria level on attentional bias toward illness-related stimuli (symptom words) and the intent to perform safety behaviors while controlling for Trait Anxiety and Health Anxiety. Participants ( …

How Getting Friendly With Bacteria Can Promote Student Appreciation Of Microbial Diversity And Their Civic Scientific Literacy, Davida S. Smyth, Simon Chen, Gina Sompanya, Molly Metz, Theresa Conefrey

Biology Faculty Publications (Archived)

ePortfolios are digital repositories where students can curate papers, projects, and reflections from individual or multiple courses across the disciplines and in a variety of formats to showcase their learning. This transparent and portable medium, which enables students to document their knowledge and abilities for assessment and career development, has been recognized by the American Association of Colleges and Universities as one of 11 high-impact practices. Using tailored rubrics, student assessment of learning gain surveys, and end-of-course exam questions, this study demonstrates how an ePortfolio assignment can be used in microbiology courses taken by majors and nonmajors to measure student …

Special Report: The Global South & Ukraine, Paul Rodell, Cathy Skidmore-Hess

Association of Global South Studies (Archived)

Contributions published in this Special Report:

• The Russian Invasion of Ukraine: Economic Impacts on the Developing World - Philip Szmedra
• The War on Ukraine and Global Food Supply: Humanitarian & Structural Considerations - William Biebuyck
• A View of African Interests in the War on Ukraine: The Past and Future of the African Struggle for Non-alignment - Aran MacKinnon and Elaine MacKinnon
• The War in Ukraine and the Middle East - Jacek Lubecki
• Dorothea A.L. Hoffman-Martin, China's Balancing Act in Response to the War in Ukraine
• Kalyan Chatterjee, Russian Invasion of Ukraine – Indian Foreign Policy Turns Full Circle
• Paul A. …

Menstrual Cycle Parameters Are Not Significantly Different After Covid-19 Vaccination, Thomas Bouchard, Mary Schneider, Madeline Schmidt, Michael D. Manhart, Richard J. Fehring

College of Nursing Faculty Research and Publications

Background:

Some studies have suggested minor changes in the menstrual cycle after COVID-19 vaccination, but more detailed analyses of the menstrual cycle are needed to evaluate more specific changes in the menstrual cycle that are not affected by survey-based recall bias.

Materials and Methods:

Using a pretest–post-test quasi-experimental evaluation of menstrual cycle parameters before and after COVID-19 vaccination, we conducted an anonymous online survey of two groups of North American women who prospectively monitor their menstrual cycle parameters daily including bleeding patterns, urinary hormone levels using the ClearBlue Fertility Monitor, or cervical mucus observations. The primary outcome measures were cycle …