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2020

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Effect Of Microwave And Roast Treatment On The Degradation Of Sulfamethazine Residue In Tilapia Meat, C.-C. Lan, B.-S. Hwang, M.-F. Tu Jul 2020

Effect Of Microwave And Roast Treatment On The Degradation Of Sulfamethazine Residue In Tilapia Meat, C.-C. Lan, B.-S. Hwang, M.-F. Tu

Journal of Food and Drug Analysis

Tilapia (Tilapia mossambica) was force-fed sulfamethazine (SMA) and residue of this antibiotic remained in the meat. We measured the change of residual SMA after microwave and roast treatment on Tilapia by different levels. The content of SMA in Tilapia increased slowly 6 hrs after feeding, had a sharp climb in 6-12 hrs and decreased gradually in 12-24 hrs. Taking the Tilapia after feeding in 1, 6, 12 and 24 hrs, we measured SMA amount in fish meat is 0.3, 0,45, 1.60 and 1.2 ppm respectively. Microwaving the fish meat after feeding in 6, 12, 24 hrs, we found SMA levels …

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Mutagenic Analysis Of Fermenting Strains And Fermented Brine For Stinky Tofu, H.-O. Chang, S.-W. Wang, J.-C. Chen, L.-F. Hsu, S.-M. Hwang Jul 2020

Mutagenic Analysis Of Fermenting Strains And Fermented Brine For Stinky Tofu, H.-O. Chang, S.-W. Wang, J.-C. Chen, L.-F. Hsu, S.-M. Hwang

Journal of Food and Drug Analysis

Stinky tofu, a popular fermented food in Taiwan and South China, is conventionally made by open-type natural fermentation in which the product quality is not easily controlled. The Food Industry Research and Development Institute (FIRDI) in Taiwan has attempted to isolate the major fermenting strains from stinky brine in order to produce stinky tofu that is sanitary and ensure its quality. This study investigated the mutagenicities of isolated fermenting strains and their fermented brine for stinky tofu by two assays: (I) Ames test and (II) mutation assay with human lymphoblast TK6 cells. In the Ames test, the number of revertants …

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Characterization Of Escherichia Coli Serotype O157 Strains Isolated In Taiwan By Pcr And Multilocus Enzyme Analysis, L.-C. Chiueh, W.-H. Shiang, D.Y.C. Shih Jul 2020

Characterization Of Escherichia Coli Serotype O157 Strains Isolated In Taiwan By Pcr And Multilocus Enzyme Analysis, L.-C. Chiueh, W.-H. Shiang, D.Y.C. Shih

Journal of Food and Drug Analysis

Escherichia coli serotype O157 strains were isolated from fecal samples of cattle and sheep, as well as raw meat in Taiwan from July 1997 to June 1999. In total, six strains of E. coli serotype O157 were collected. Of these, only one strain, isolated from fecal samples of sheep, was characterized as E. coli O157:H7. The remaining five strains were determined as H41, H45, H? (non-typeable with 43 O-antisera) and NM (non-motile). All six strains were analyzed for pathogenic genes of Enterohemorrhagic E. coli (EHEC) by the Polymerase chain reaction (PCR) method. The E. coli O157:H7 was the only strain …

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Pharmacological Functions Of Chinese Medicinal Fungus Cordyceps Sinensis And Related Species, S.-Y. Wang, M.-S. Shiao Jul 2020

Pharmacological Functions Of Chinese Medicinal Fungus Cordyceps Sinensis And Related Species, S.-Y. Wang, M.-S. Shiao

Journal of Food and Drug Analysis

Cordyceps sinensis, an entomogenous fungus used in traditional Chinese medicine, exhibits very broad biological and pharmacological actions in hepatic, renal, cardiovascular, and immunologic systems as well as anticancer activity. Pharmacological functions of Cordyceps are primarily due to the bioactive polysaccharides, modified nucleosides, and cyclosporin-like metabolites produced by this fungus and related species. The beneficial effects on renal and hepatic function and immunomodulation-related antitumor activities are most promising and deserve great attention. Many previous studies used fruiting bodies, but recently an increasing number of studies have used cultured mycelia in investigations. It is difficult to determine if the same bioactive ingredients …

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Elisa, Western Blotting, Immunocytolocalization And Immunoaffinity Column For Naturally Occuring Bioactive Compounds Using Monoclonal Antibodies, S.-J. Shan, W. Putalun, N. Fukuda, O. Morinaga, H. Tanaka, Y. Shoyama Jul 2020

Elisa, Western Blotting, Immunocytolocalization And Immunoaffinity Column For Naturally Occuring Bioactive Compounds Using Monoclonal Antibodies, S.-J. Shan, W. Putalun, N. Fukuda, O. Morinaga, H. Tanaka, Y. Shoyama

Journal of Food and Drug Analysis

Anti-sennoside A (SA) and anti-glycyrrhizin (GC) MAbs were used for screening and cloning. And they were also used for ELISA. New western blotting method of determination for GC and ginsenoside was established that compounds separated by silica gel TLC were transferred to a polyvinylidene difluoride (PVDF) membrane. The membrane was treated with NaIO4 solution followed by BSA, resulting in a ginsenoside-BSA conjugate on the PVDF membrane which was stained by the general western blotting. Immunocytolocalization of GC in the fresh root of Glycyrrhiza species was conducted using anti-GC MAb after blotting to PVDF membrane. Immunoaffinity column chromatography using anti-ginsenoside Rb1 …

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Some Transpacific Thoughts On The Regulatory Need For Standardization Of Herbal Medical Products, L.A. Mitscher, S. Pillai, D.M. Shankel Jul 2020

Some Transpacific Thoughts On The Regulatory Need For Standardization Of Herbal Medical Products, L.A. Mitscher, S. Pillai, D.M. Shankel

Journal of Food and Drug Analysis

The history and present status of herbal products/neutraceuticals in The United States is briefly reviewed. A case is presented for further, more comprehensive, regulatory oversight than is presently the circumstance under the DSHEA act.

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Preparative Isolation And Gas Chromatography-Mass Spectrometry Analysis Of Triterpenoids In Kansui Radix, J.-H. Lin, Y.-R. Ku, Y.-T. Lin, S.-F. Teng, K.-C. Wen, C.-H. Liao Jul 2020

Preparative Isolation And Gas Chromatography-Mass Spectrometry Analysis Of Triterpenoids In Kansui Radix, J.-H. Lin, Y.-R. Ku, Y.-T. Lin, S.-F. Teng, K.-C. Wen, C.-H. Liao

Journal of Food and Drug Analysis

Two triterpenoids, euphol and tirucallol were isolated from Kansui Radix (Euphorbia kansui Liou, Euphorbiaceae) by a modified isolation method. These constituents were used as marker substances for qualffy control of Kansui Radix using a home-developed gas chromatography-mass spectrometry (GC-MS) method. The linear calibranon ranges were 50.0-400.0 μg/mL (r=0.9988) for euphol and 20.0-200.0 μg/mL (r=0.9976) for tirucallol. The relative standard deviations of two marker substances for intraday and interday analyses were 0.90-3.07% and 3.93-8.17%, respectively. Recoveries were euphol 98,3±1.2%, and tirucallol 84.8±.0%. The contents of two marker substances in seven crude drugs of Kansui Radix were euphol 0.10-0.19%, and drucallol 0.05-0.07%.

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Determination Of Unbound Hesperetin In Rat Blood And Brain By Microdialysis Coupled To Microbore Liquid Chromatography, T.-H. Tsai, Y.-F. Chen Jul 2020

Determination Of Unbound Hesperetin In Rat Blood And Brain By Microdialysis Coupled To Microbore Liquid Chromatography, T.-H. Tsai, Y.-F. Chen

Journal of Food and Drug Analysis

A rapid and sensitive microbore high-performance liquid chromatography (HPLC) coupled to microdialysis technique for simultaneous determination of unbound hesperetin in rat blood and brain has been developed. The HPLC assay was carried out using a microbore reversed-phase C-18 column (100 × 1 mm I.D., 5 μm particle size). The mobile phase used was acetonitrile-methanol-20 mM monosodium phosphate (pH 5.3) (40:10:50, v/v/v) for hesperetin at a flow-rate of 0.05 mL/min. The assay was performed by monitoring the wavelength of maximum UV absorbance at 288 nm. Microdialysis probes were inserted into the jugular vein/right atrium and brain striatum of male Sprague-Dawley rats. …

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Method Development For Evaluation Of Hepato-Protection Functionality Of Health Food, C.-M. Hu, H.-W. Cheng Jul 2020

Method Development For Evaluation Of Hepato-Protection Functionality Of Health Food, C.-M. Hu, H.-W. Cheng

Journal of Food and Drug Analysis

Proper regulation of the acclaimed functionality and safety of the health food is a consensus among the health authorities of every nation. The objective of this study is focused on the methods development for safety and functionality of hepatic injury prevention of health food using CCl4-induced hepatotoxicity as model. The result from this study could provide some useful information for setting a feasible and reasonable evaluating method for regulatory purpose. In this study, we have shown that in the untreated rats, administration of CCl4 at 0.05 and 0.20 mL/kg, BW caused the increase of SGOT activities from 118 ± 25 …

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Biosynthesis Of Folates By Streptococcus Thermophilus And Lactobacillus Delbruekii Ssp. Bulgaricus, M.-Y. Lin, C.-M. Young Jul 2020

Biosynthesis Of Folates By Streptococcus Thermophilus And Lactobacillus Delbruekii Ssp. Bulgaricus, M.-Y. Lin, C.-M. Young

Journal of Food and Drug Analysis

The folate synthesizing ability of yogurt bacteria, including Streptococcus thermophilus MC and ATCC 19258 and Lactobacillus delbrueckii ssp. bulgaricus 448 and 449, were evaluated. The major effort of this study focused on increasing the folate contents of yogurt bacteria fermented milk by adding lactose or calcium chloride. The addition of 2% lactose in reconstituted non-fat dry milk increased folate synthesis. Although folates increased only about 3 to 7% for yogurt cultures incubated for 6 hr, folates increased 12 to 198% for yogurt bacteria grown for 18 hr. The folate content decreased for reconstituted milk fermented with S. thermophilus MC or …

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Analysis Of Bentazon And Its Metabolites In Urine And Blood, M.-F. Chen, H.-P. Li, S.-S. Wong Jul 2020

Analysis Of Bentazon And Its Metabolites In Urine And Blood, M.-F. Chen, H.-P. Li, S.-S. Wong

Journal of Food and Drug Analysis

A rapid analysis method of herbicide bentazon and its metabolites (6-OH-bentazon and 8-OH-bentazon) in urine and blood has been developed. Automatic solid phase extraction and HPLC/UV were used to extract and analyze pesticides. The recoveries of bentazon, 6-OH-bentazon and 8-OH-bentazon in urine spiked with 2 μg of standards were 62.16 ± 3.05%, 59.01 ± 3.19%, and 109.93 ± 0.01%, respectively. In blood, the recoveries were 119.45 ± 3.46%, 138.38 ± 6.72%, and 131.02 ± 3.86%, respectively. The detection limits of bentazon, 6-OH-bentazon and 8-OH-bentazon in urine are 0.047, 0.052, and 0.024 ppm, respectively. Gas chromatography-mass spectrometry (GC-MS) was also used …

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Characteristics And Mutagenicity Of Fumes Obtained From Commercial Edible Oils, S.-C. Wu, G.-C. Yen Jul 2020

Characteristics And Mutagenicity Of Fumes Obtained From Commercial Edible Oils, S.-C. Wu, G.-C. Yen

Journal of Food and Drug Analysis

Seven commercial edible oils including soybean oil, corn germ oil, sunflower oil, peanut oil, blend peanut oil, calola oil and lard were investigated for their physical and chemical properties as well as for the mutagenicity of oil fumes by applying the Ames test. The smoking points of those oils were 118, 119, 95, 98, 107, 138 and 137°C, respectively. Lard had the best oxidative stability among those seven oils as determined by the Rancimat method. Peanut oil produced the largest amount of fumes while corn germ oil and sunflower oil produced the least amounts. The oil fumes (0-10 μg/plate) of …

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Hplc Analysis Of N-Acetyl-Chito-Oligosaccharides During The Acid Hydrolysis Of Chitin, K.L.B. Chang, J. Lee, W.-R. Fu Jul 2020

Hplc Analysis Of N-Acetyl-Chito-Oligosaccharides During The Acid Hydrolysis Of Chitin, K.L.B. Chang, J. Lee, W.-R. Fu

Journal of Food and Drug Analysis

The hydrolysis of chitin (93% N-acetylation) by hydrochloric acid produced N-acetylchito-oligosaccharides (NACOs) with low degrees of polymerization (DP) from 2 to 6. The HPLC retention time of NACOs decreased with increased water content in the mobile phase. A gradient elution procedure, using acetonitrile to water (v/v) ratio lowered linearly from 80/20 to 60/40 within 60 min, provided optimal resolution and characterization for the NACOs. The natural logarithm of the retention time of NACOs correlated linearly with the DP values. Hydrolysis of chitin followed a series-parallel reaction mechanism. At the same time the NACOs with higher DP's were generated from chitin, …

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The Relationship Between The Cold Chain System And Vaccine Potency In Taiwan: (Ii) Oral Polio Vaccine, D.-Y. Wang, R.-I. Yang, Y.-C. Yang, S.-Y. Yeh, T.-L. Chen, H.-F. Cheng, J.-T. Hsieh, C.-P. Lin Jul 2020

The Relationship Between The Cold Chain System And Vaccine Potency In Taiwan: (Ii) Oral Polio Vaccine, D.-Y. Wang, R.-I. Yang, Y.-C. Yang, S.-Y. Yeh, T.-L. Chen, H.-F. Cheng, J.-T. Hsieh, C.-P. Lin

Journal of Food and Drug Analysis

The live attenuated oral poliovirus vaccine (OPV) has been used for the poliomyelitis eradication program in Taiwan for a long time. Since it is manufactured from a highly thermolabile vaccine strain of poliovirus, it should be stored under the recommended temperature of -20°C or below. This study investigated and evaluated the efficiency of the cold chain system for maintaining the polio vaccine potency in Taiwan. We selected several health stations and local hospitals/pediatric offices in each County/City of Taiwan to take the OPV samples and test their potency from Nov. 1997 to Feb. 1998. Our results showed that the type-1 …

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Investigation For Contamination Of Parasite And Aerobic Bacteria In Frozen Tilapia Fillets In Taiwan, J.-W. Shin, Y.-H. Huang Jul 2020

Investigation For Contamination Of Parasite And Aerobic Bacteria In Frozen Tilapia Fillets In Taiwan, J.-W. Shin, Y.-H. Huang

Journal of Food and Drug Analysis

Three hundred frozen tilapia fillet samples obtained from shopping malls, supermarkets and conventional markets from different geographical areas of Taiwan in the period July 1997 to June 1998 were screened for parasite and microorganism infections. Results showed that no samples were infected with parasitic metacercaria or larva, nor with Vibrio. However, 0.36% test samples were found to be contaminated with Micrococcus or Aeromonas. The average aerobic bacteria counts for all the test samples were 1.2 × 105 CFU/g; samples obtained from northern, central and southern Taiwan were 7.8 × 104, 1.4 × 105 and 1.5 × 105 CFU/g, respectively, and …

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Determination Of Folates Synthesized By Lactic Acid Bacteria, C.-M. Young, M.-Y. Lin Jul 2020

Determination Of Folates Synthesized By Lactic Acid Bacteria, C.-M. Young, M.-Y. Lin

Journal of Food and Drug Analysis

Eight strains of lactic acid bacteria, including Bifidobacterium longum B6 and 15708, Lactobacillus acidophilus N-1 and 4356, Lactobacillus bulgaricus 448 and 449, and Streptococcus thermophilus MC and 573 were investigated for their folate synthesizing ability by applying the high-performance liquid chromatographic method. The results showed that lactic acid bacteria synthesized maximum levels of folate in six hours in fermented milk. B. longum B6 demonstrated the highest folate synthesizing ability, and B. longum 15708 also produced folate satisfactorily. These two strains increased folate 3.5 and 2.4 fold, respectively, after 6h fermentation at 37°C. 5-Methyltetrahydrofolate was the main derivative of folate found …

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Analysis, Formation And Inhibition Of Cholesterol Oxidation Products In Foods: An Overview (Part I), C.-Y. Tai, Y.C. Chen, B.H. Chen Jul 2020

Analysis, Formation And Inhibition Of Cholesterol Oxidation Products In Foods: An Overview (Part I), C.-Y. Tai, Y.C. Chen, B.H. Chen

Journal of Food and Drug Analysis

Cholesterol oxidation products (COPs) formed in cholesterol-containing foods during heating or illumination have been found to impart a potential hazard to health. Numerous studies have indicated that COPs may have several adverse biological effects, such as mutagenicity, carcinogenicity, angiotoxicity, cytotoxicity, atherogenicity, atherosclerosis, cell membrane damage and inhibition of cholesterol biosynthesis. Therefore, the safety of COPs has become a major concern for the public. This paper is an overview of analysis, formation and inhibition of COPs in foods. COPs are routinely extracted by organic solvents, followed by saponification and solid phase extraction for enrichment of COPs, and separation and identification by …

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A Rapid Gas Chromatographic Method For Direct Determination Of Free Sterols In Animal And Vegetable Fats And Oils, Y.-M. Choong, H.-J. Lin, C.-W. Chen, M.-L. Wang Jul 2020

A Rapid Gas Chromatographic Method For Direct Determination Of Free Sterols In Animal And Vegetable Fats And Oils, Y.-M. Choong, H.-J. Lin, C.-W. Chen, M.-L. Wang

Journal of Food and Drug Analysis

In this study, a rapid and simple method without a derivatization process for determination of the free sterols in vegetable and animal oils was developed. A direct injection gas chromatography with a non-polar megabore column (DB-1, 5 m × 0.53 mm, 1μm) was used. The recoveries of cholesterol, campesterol, stigmasterol and β-sitosterol from lard were at the range of 89%-106% with a coefficient of variation less than 12%. Total sterols were determined using the same method after saponification of the oil sample. Fourteen samples, including olive oil, sunflower oil, peanut oil, soybean oil, sesame oil, lard and egg yolk oil, …

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Effects Of The Application Of Hydrogen Peroxide On The Preservation Of Firm Tofu, H.-W. Chang, W.-L. Chen Jul 2020

Effects Of The Application Of Hydrogen Peroxide On The Preservation Of Firm Tofu, H.-W. Chang, W.-L. Chen

Journal of Food and Drug Analysis

In this study, hydrogen peroxide concentrations for treatment of firm tofu was investigated. Because firm tofu has high moisture content, high water activity, and is a low-acid food, it is difficult to preserve. Hydrogen peroxide treatment could possibly be a good preservative. The analytical methods for hydrogen peroxide, obtained regression equations by the peroxidase method and Merck RQflex. Reflectometer methods were Y=0.314X-0.0845 (R2=0.998) and Y=1.03X-0.438 (R2=0.977), respectively. These were accurate methods for hydrogen peroxide analysis, with the Merck RQflex Reflectometer method being the more convenient method. The shelf-life at room temperature of firm tofu is 1 day without hydrogen peroxide …

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Effect Of Dietary Vitamin D3 On T Helper-Related Activities Of Lupus-Prompt New Zealand Black/New Zealand White F1 Mice, B.-C. Yang, C.-W. Liu, C.-K. Yu Jul 2020

Effect Of Dietary Vitamin D3 On T Helper-Related Activities Of Lupus-Prompt New Zealand Black/New Zealand White F1 Mice, B.-C. Yang, C.-W. Liu, C.-K. Yu

Journal of Food and Drug Analysis

We investigated the effect of dietary vitamin D3 on T helper-related activities and progress of lupus in NZB/NZW F1 mice. Mice were fed with 0.4 or 2 μg vitamin D3 dissolved in 50 μL olive oil every other day. Expression of T cell-related cytokine genes including those of IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 and IFN-γ was monitored during the disease course by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). In addition, total serum IgG1 and IgG2a and anti-dsDNA antibody were measured. We observed a prolonged survival of mice receiving 2μg of vitamin D3 as compared with control mice. However, …

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Determination Of Copper In Edible Oils By Direct Graphite Furnace Atomic Absorption Spectrometry, S.-S. Chen, C.-M. Chen, C.-C. Cheng, S.-S. Chou Jul 2020

Determination Of Copper In Edible Oils By Direct Graphite Furnace Atomic Absorption Spectrometry, S.-S. Chen, C.-M. Chen, C.-C. Cheng, S.-S. Chou

Journal of Food and Drug Analysis

A simple method for determination of copper in edible oils was developed, using a polarized Zeeman graphite furnace atomic absorption spectrophotometer and a pyro graphite tube. Edible oils were diluted with 2% lecithin-cyclohexane and analyzed directly by the standard addition method. The optimal ashing and atomizing temperatures were 900°C and 2700°C, respectively. A standard solution diluted with 2% lecithin-cyclohexane was superior to salad oil or cyclohexane. Results were best measured when the concentration of lecithin in cyclohexane was higher than 2%. The detection limit of this method was 2.0 ng/mL. The respective range of recovery of copper was 85.5-90.0% and …

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The Relationship Between The Cold Chain System And Vaccine Potency In Taiwan: (I) Live Measles Vaccine And Mmr Vaccine, D.-Y. Wang, R.-I. Yang, Y.-C. Yang, S.-Y. Yeh, T.-L. Chen, H.-F. Cheng, J.-T. Hsieh, C.-P. Lin Jul 2020

The Relationship Between The Cold Chain System And Vaccine Potency In Taiwan: (I) Live Measles Vaccine And Mmr Vaccine, D.-Y. Wang, R.-I. Yang, Y.-C. Yang, S.-Y. Yeh, T.-L. Chen, H.-F. Cheng, J.-T. Hsieh, C.-P. Lin

Journal of Food and Drug Analysis

Taiwan has a long history of using vaccines in order to protect people against certain infectious diseases. Several types of vaccines have been used successfully for protecting children from poliomyelitis, measles, and other acute infectious diseases. However, for the monovalent measles vaccine (MV), and the trivalent measles-mumps-rubella combined vaccine (MMR vaccine) which are made from live attenuated viruses, they are unstable unless stored at a proper low temperature, at a certain pH level, in darkness, and combined with some specific stabilizers. Maintaining these live virus vaccines at the recommended temperatures of +2 to +8°C is one of the most important …

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The Κ-Opioid Receptor In Opioid Dependence, W. Wongchanapai, B.K. Tsang, I.K. Ho Jul 2020

The Κ-Opioid Receptor In Opioid Dependence, W. Wongchanapai, B.K. Tsang, I.K. Ho

Journal of Food and Drug Analysis

Opioid dependence that particularly mediates through the μ-opioid receptor remains a major concern of opioid analgesics. Drugs which interact with κ-opioid receptors are increasingly used as an alternative to μ- agonist analgesic. Several studies have reported that chronic administration of κ-opioid agonists such as U-50488H, U-69,593, and butorphanol also results in development of physical dependence/withdrawal. In addition, the ability of a highly selective κ-opioid antagonist, nor-binaltorphimine, given systemically or spinally, to precipitate withdrawal behaviors in opioid-dependent animals further demonstrates that both supraspinal and spinal sites of κ-opioid receptors play an important role in opioid dependence/withdrawal. With regard to the role …

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Authentication Of Chinese Medicinal Materials By Dna Technology, P.-C. Shaw, F.-N. Ngan, P.P.-H. But, J. Wang Jul 2020

Authentication Of Chinese Medicinal Materials By Dna Technology, P.-C. Shaw, F.-N. Ngan, P.P.-H. But, J. Wang

Journal of Food and Drug Analysis

Chinese medicinal materials are widely used in the Orient and have found increased popularity in the West as an alternative source of health care. Traditionally, authentication of these materials relies on organoleptic methods. Progress in pharmacognosy has capitalised on the anatomical and chemical profiles as markers for authentication. The advancement of techniques in molecular biology has provided new means for examining genotypic characteristics. Since 1990, several research groups have employed polymerase chain reaction based methods, restriction fragment length polymorphism and DNA sequencing to authenticate medicinal materials and to define the phylogenetic relationship of related species. This review provides an account …

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Determination Of Melatonin In Rat Plasma By High Performance Liquid Chromatography With Electrochemical Detection, S.-T. Chou, T.-F. Perng Jul 2020

Determination Of Melatonin In Rat Plasma By High Performance Liquid Chromatography With Electrochemical Detection, S.-T. Chou, T.-F. Perng

Journal of Food and Drug Analysis

Melatonin ( 5-methoxy-N-acetyltryptamine ) plays a role in a number of distinct physiological functions. We have developed a convenient high performance liquid chromatography assay with electrochemical detection ( HPLC / ECD ) for the accurate determination of melatonin in biological samples. This assay involves direct addition of rat plasma to acetonitrile for simultaneous protein precipitation and melatonin extraction. The assay was validated by addition of melatonin to rat plasma with a recovery rate between 92 and 99 %. The correlation coefficient of calibration curve was 1.000. The relative standard deviations for intraday and interday analyses were 1.5-2.2 % and 3.5-5.0 …

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A Screening Program On Anti-Metastasis From Microbial Metabolites, Y.-H. Lin, C.-H. Tang, C.-C. Chen, S.-S. Chen, C.-Y. Chen, G.-F. Yuan, S.-M. Hwang Jul 2020

A Screening Program On Anti-Metastasis From Microbial Metabolites, Y.-H. Lin, C.-H. Tang, C.-C. Chen, S.-S. Chen, C.-Y. Chen, G.-F. Yuan, S.-M. Hwang

Journal of Food and Drug Analysis

A malignant tumor is hard to eradicate by removing the mass surgically because the abnormal cells often metastasize. How to find and kill these metastatic cells is an important key to cure cancer. We have isolated over 100 filamentous fungi from soil of extreme environments in Taiwan, including forest, hot spring, lake and fruit tree rhizosphere in order to screen some biologically active compounds. Screening of anti-metastatic compounds, we used a set of highly metastatic cell lines, B16-F10 (mouse, melanoma) and HT-1080 (human, fibrosarcoma) and cell lines with low metastatic ability, B 16-F1 (mouse, melanoma) and MRC-5 (human, lung, normal …

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Comparative Study Of Antioxidant Activity Of Grape (Vitis Vinifera) Seed Powder Assessed By Different Methods, H. Li, X. Wang, P. Li, Y. Li, H. Wang Jul 2020

Comparative Study Of Antioxidant Activity Of Grape (Vitis Vinifera) Seed Powder Assessed By Different Methods, H. Li, X. Wang, P. Li, Y. Li, H. Wang

Journal of Food and Drug Analysis

The phenolic content and antioxidant activity of grape (Vitis vinifera) seed powder extracted by in vitro physiological procedure and chemical procedure were investigated. The antioxidant potential of the extract were assessed by employing different in vitro assays such as CUPRAC, DPPH, ABTS, OH radical scavenging capacity, and peroxidation inhibiting activity. The in vitro physiological procedure yielded a higher phenolic content and antioxidant capacity than the chemical procedure. As for digestive enzymatic extracts, phenolic content and antioxidant capacity of the dialysates of grape seed powder were lower than those of the retentates. As for solvents extracts, extraction with acetone:water (70:30) led …

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Simultaneous Determination Of Podophyllotoxin, Quercetin And Kaempferol In Podophyllin By Liquid Chromatography Tandem Mass Spectrometry, M.-C. Lin, J.-H. Lin, S.-K. Chen, Y.-W. Cheng, H.-W. Cheng Jul 2020

Simultaneous Determination Of Podophyllotoxin, Quercetin And Kaempferol In Podophyllin By Liquid Chromatography Tandem Mass Spectrometry, M.-C. Lin, J.-H. Lin, S.-K. Chen, Y.-W. Cheng, H.-W. Cheng

Journal of Food and Drug Analysis

A quantitative high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) was developed to analyze the constituents of podophyllin. Chromatographic separation was performed on a Cosmosil 5C18-MS (25 cm × 4.6 mm ID., 5 μm) reverse phase column using a gradient of mobile phase (0.25% formic acid-methanol). The column effluent was split 2: 3 into the photodiode detector and tandem mass spectrometer. Podophyllotoxin, quercetin and kaempferol in podophyllin were identified by daughter ion scan mode and then determined their contents by multiple-reaction monitoring (MRM) mode. The limits of detection and quantitation for podophyllotoxin, quercetin and kaempferol were 2.40, 8.01; 2.94, 9.87 and …

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Method Development And Validation For The Gc-Fid Assay Of Ethanol In Reservoir-Type Fentanyl Transdermal Patches, K.-H. Lu, C.-Y. Chen, R.-L. Shih, F.-S. Chin, C.-S. Chien Jul 2020

Method Development And Validation For The Gc-Fid Assay Of Ethanol In Reservoir-Type Fentanyl Transdermal Patches, K.-H. Lu, C.-Y. Chen, R.-L. Shih, F.-S. Chin, C.-S. Chien

Journal of Food and Drug Analysis

With ethanol acting as a skin permeation enhancer in the reservoir-type fentanyl transdermal delivery system, accurate assay of ethanol in the adapted cosolvent system is an important quality control component. This paper describes the development and validation of an isothermal gas chromatography-flame ionization detection (GC-FID) method for the assay of ethanol in reservoir-type fentanyl patches (Durogesic®). Samples were extracted with water by ultrasonic vibration using acetone as the internal standard. Using a polar column (Supelco OVI-G43, 30 m × 0.53 mm) for the gas chromatographic analysis, ethanol and excipients were well resolved. The method was fully validated according to the …

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A Rapid Flow Injection Spectrophotometric Analysis For Tetracycline Chlortetracycline Or Oxytetracycline, W. Ruengsitagoon Jul 2020

A Rapid Flow Injection Spectrophotometric Analysis For Tetracycline Chlortetracycline Or Oxytetracycline, W. Ruengsitagoon

Journal of Food and Drug Analysis

A rapid flow injection (FI) spectrophotometric procedure for the determination of tetracycline(TC), chlortetracycline(CTC) or oxytetracycline(OTC) has been developed. The method is based on the injection of 150 μL standard or sample solution into a reagent stream of iron (III) (500 μg/mL in 5.0 × 10-3 mol/L HNO3) with the optimum flow rate of 4.0 mL/min. The brown iron (III)-tetracycline complex is monitored at 423 nm. The flow injection system and the experimental conditions were optimized by means of univariate method. Under the optimum conditions, calibration graphs were obtained for 1.0 - 100.0 μg/mL and the detection limits (s/n = 3) …

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