Digital Commons Network^™

Cardiovascular Adaptation To Whole-Body Vibration Exercise In Obese Young Adults: A Systematic Review, Meta-Analysis, And Exploratory Analysis Of Sex Differences, Rafiu Okuneye, Abdulkareem Taiwo, Fatai Akinola Apalara, Okechukwu Kingsley Oforka, Isaiah Whenayon Ajoseh, Ogar Emmanuel Ekawu

Indonesian Journal of Research in Physical Education, Sport, and Health

Background: Obesity is strongly associated with hypertension and autonomic dysfunction. Whole-body vibration exercise (WBVE) has been proposed as a low-impact intervention for improving cardiovascular function in obese adults, but sex-specific responses remain unclear. Objective: This study aimed to evaluate the effects of whole-body vibration exercise on blood pressure, autonomic function, and cardiac output in adults with obesity, and to explore potential sex differences in cardiovascular adaptations. Methods: We conducted a systematic review and meta-analysis of randomized controlled trials and quasi-experimental studies examining the effects of WBV on systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate variability (HRV), and …

Physical Fitness, Body Mass Index, And Physical Activity Among Senior Secondary Students: A Cross-Sectional Study In An Indonesian Islamic Secondary School, Ilona Pratiwi Hutabarat, Acep Rohmat Nurhidayat, Aprizal Fikri, Teguh Andi Prabowo, I Bagus Endrawan

Indonesian Journal of Research in Physical Education, Sport, and Health

Background: Physical fitness is a fundamental component of adolescent health and contributes to academic performance, mental well-being, and lifelong health outcomes. Following the COVID-19 pandemic and the rise in sedentary behavior, evidence on the fitness profiles of Indonesian senior secondary students—particularly in Islamic secondary schools (Madrasah Aliyah)—remains scarce. Objective: To assess the physical fitness level of Madrasah Aliyah students and to examine its association with body mass index (BMI) and self-reported physical activity. Methods: A cross-sectional survey was conducted among 35 students (18 male, 17 female; aged 15–19 years) at MA Raudlatul Irfan, South Tangerang, using purposive sampling. Physical fitness …

Dietary Patterns And Nutritional Status In Soccer Athletes: A Cross-Sectional Study From An Ergonomic Perspective, Rina Mayangsari, Ravi Masitah

Indonesian Journal of Research in Physical Education, Sport, and Health

Background: Normal nutritional status has been established as one of the principal determinants in an athlete's performance. Dietary quality plays a critical role in optimizing athletes' physical fitness and performance outcomes. Objective: To examine the associations between dietary patterns on the MacPersona System and nutritional status of soccer players (energy, protein, et cetera, fat, and carbohydrates). Methods: A cross-sectional quantitative study was conducted involving 21 soccer players (total sampling). Dietary patterns were assessed using a validated Semi-Quantitative Food Frequency Questionnaire (SQ-FFQ). Nutritional status was measured using Body Mass Index(BMI) based on anthropometric data. The chi-square test was used for statistical …

Cardiorespiratory Fitness In Indonesian Adolescents: Limited Role Of Bmi And Dominant Influence Of Age And Sex In A Large Cross-Sectional Study, Anies Setiowati, Sri Sumartiningsih, Nur Ilhaini Sucipto, Fatih Hazar, Philip X. Fuchs

Indonesian Journal of Research in Physical Education, Sport, and Health

Background: Cardiorespiratory fitness (CRF) is a crucial indicator of long-term cardiovascular and metabolic health. Whether body mass index (BMI) independently predicts CRF beyond demographic factors in large, real-world adolescent cohorts remains inconsistently defined. Objective: To determine the independent contributions of body mass index (BMI), age, and sex to cardiorespiratory fitness among Indonesian adolescents using a large community-based dataset.. Methods: A cross-sectional study was conducted using community-based fitness screening data from Central Java, Indonesia. A total of 2,730 adolescents aged 10-18 years (mean 13.3 +/- 2.2 years; 49.6% male) were included. The primary outcome was CRF measured by the 1,600-meter run …

The Intricate Dance Of Emotions And Psychophysiology: Unveiling The Secrets Of Microexpressions, Jaiteg Singh, Deepika Sharma, Babar Shah, Sukhjit Singh Sehra, Farman Ali, Irfan Hussain

All Works

Background: Emotion recognition plays a pivotal role in behavioral analysis, mental health assessment, and human-computer interaction. Micro-expressions, which are brief and involuntary facial movements, offer valuable insights into concealed emotions. However, validating micro-expressions remains a challenge due to their subtlety and short duration. This study aims to enhance the validation and classification of micro-expressions by integrating electromyogram (EMG) signals with facial action units (AUs). Methods: EMG data was collected using the EMG Muscle Sensor Module V3.0, interfaced with an Arduino Mega 2560 microcontroller. To ensure signal clarity, various data filtration techniques were applied to eliminate noise, motion artifacts, and baseline …

Lgr5: From Stem Cell Marker To Therapeutic Target, Peyton C High, Maya G Cappellino, Tiffani A Blackburn, Kendra S Carmon

Faculty, Staff and Student Publications

Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) is an established marker of normal and cancer stemlike cells. LGR5 has been implicated in promoting cancer cell plasticity that drives tumorigenesis, metastasis, and therapeutic resistance. LGR5 rapidly and constitutively internalizes and potentiates Wnt (Wingless/Int-1)/β-catenin and adhesion signaling pathways, though its precise mechanisms and interacting partners remain unresolved. An improved understanding of LGR5 signaling may provide invaluable insight into its intricate and important functions in cancer progression. Moreover, several LGR5-targeting therapies, including peptibody- and antibody-drug conjugates and bispecific antibodies, are showing promising efficacy and tolerability in colorectal cancer and other tumor types. This …

Mitochondrial Presequences Harbor Variable Strengths To Maintain Organellar Function, Youmian Yan, Baigalmaa Erdenepurev, Thiago N Menezes, Ian Collinson, Natalie M Niemi

2020-Current year OA Pubs

Hundreds of mitochondrial proteins rely on N-terminal presequences for organellar targeting and import. While generally described as positively charged amphiphilic helices, presequences lack a consensus motif and thus likely promote protein import into mitochondria with variable efficiencies. Indeed, the concept of presequence strength underlies biological models such as stress sensing, yet a quantitative analysis of what dictates strong versus weak presequences is lacking. Furthermore, the extent to which presequence strength affects mitochondrial function and cellular fitness remains unclear. Here, we capitalize on the MitoLuc protein import assay to define multiple aspects of presequence strength. We find that select presequences, including …

Telaah Kritis Mengenai Perspektif Historis-Evolusioner Dalam Studi Hukum Dan Perkembangan Nasional Di Indonesia, Jimly Asshiddiqie, S.H., M.H.

Jurnal Hukum & Pembangunan

Abstract

Kemungkinan Terjadinya Pencemaran Akibat Penambangan Dasar Laut Dalam Dl Laut Banda Ditinjau Dari Segi Hukum Lingkungan, H. Ruhulessin, S.H.

Jurnal Hukum & Pembangunan

Abstract

Quantifying Dag-Ness: A Multi-Component Framework, Erik Csikos

Northeast Journal of Complex Systems (NEJCS)

Directed acyclic graphs (DAGs) are important structures across many disciplines, including mathematics and network science. They are especially useful in modeling causal and hierarchical relationships in a wide variety of applications. The primary
features of a DAG, directedness and acyclicity, are traditionally treated as binary. A graph is either DAG or it is not. Because of this, graphs are not usually interpreted as partially acyclic or partially directed. To address this gap in understanding, we
introduce a framework, consisting of five components, that will provide a continuous measure of DAG-ness. This measure will take into account edge acyclicity, node cyclicity, …

International Student Transition: An Autoethnographic And Archival Study, Abdulsamad Olajide Yusuf, Jepkorir Rose Chepyator-Thomson

Journal of Comparative & International Higher Education

Within the framework of a globalized university campus, this study investigates the process that an international student goes through while adapting to a new culture. Using autoethnography and archival research methods, this study recounts the personal and lived experiences of a doctoral international student navigating the cultural adjustment contexts of a southeastern public university, providing an understanding of how the challenges and opportunities faced by international students are historicized and located within a broader cultural and social context. This approach includes maintaining a reflexive journal, self-analysis, personal conversations, and analyzing historical records and correspondences, including policy papers, factbooks, and institutional …

Administering Surveys To International Undergraduates—Recommendations For Best Practices, Ricardo Paniagua, Barry Fass-Holmes

Journal of Comparative & International Higher Education

Researchers and educators historically have administered surveys to collect data, track perceptions, and/or solicit opinions from undergraduates, including internationals, attending American institutions. To promote survey responses’ generalizability, the present report advocates the following recommendations for best practices in survey administration. 1) Use stratified random sampling methodology to identify which specific subset of students from the campus’ source population to invite to the survey. 2) Strive for, design, and implement an administration process that achieves a high response rate (preferably above 90%). 3) Choose an incentive that is truly salient for survey invitees to recruit and reward them for their time. …

Epidemiological Survey Of Abortion In Camels (Camelus Dromedarius) And Histopathological Investigation Of Toxoplasmosis And Neosporosis In Those Animals From Slaughterhouses In Southeastern Algeria, Amira Djeddou-Benabid, Hind Houssou, Omar Bennoune, Ferhat Nouicer, Amel Djaout

The Thai Journal of Veterinary Medicine

Toxoplasma gondii and Neospora caninum are heteroxenous apicomplexan parasites recognized as major causes of reproductive disorders in numerous mammalian species. The present study aimed to assess through an epidemiological survey, the prevalence of reproductive losses in 75 dromedary herds during the period 2022-2025, as well as the associated risk factors, and to detect these protozoa by histopathological analysis in 63 dromedaries slaughtered at the slaughterhouse of El Oued province (126 samples: 63 hearts and 63 diaphragms). The survey revealed that 80% of the herds had a history of abortions, of which 60% also reported cases of stillbirth. Risk factor analysis …

The Effect Of The Mind Diet On Cognitive Decline In Als Patients With Frontotemporal Dementia, Riya Thomas, Reba Thomas, Eliana Gitelman

Department of Nutrition Student Projects

Amyotrophic lateral sclerosis (ALS) is a progressive disease that affects nerve cells in the brain and the spinal cord, leading to a broad range of neurological issues. Individuals with ALS experience progressive muscle weakness, dysphagia, slurred speech, and impaired movement and cognition. In addition to physical symptoms, around 15-20% of individuals with ALS also develop frontotemporal dementia (FTD). There are limited interventions to slow cognitive decline in this specific population. The Mediterranean-DASH Intervention for Neurodegenerative Delay (MIND) diet has been associated with slower cognitive decline and reduced risk in neurodegenerative disease among older adults; however, its effects have not been …

Comparative Evaluation Of Irrigants On Sealer Penetration: An In Vitro Confocal Laser Scanning Microscopy Study, Savani Purohit, Priyanka Zinge, Roshan Samuel, Aayush Bhatt, Rucha Pawar

International Arab Journal of Dentistry

Objectives: Effective smear layer removal enhances sealer penetration in endodontics. Chelating agents vary in their efficacy, influencing treatment outcomes. In this study, the depth of penetration of AH Plus and Bio-C root canal sealers into dentinal structures was analysed using confocal laser scanning microscopy (CLSM), following the application of four final irrigants: 17% EDTA, 20% citric acid, 0.2% chitosan, and 5% fulvic acid.

Methods: Ninety-six single-rooted human premolars were prepared and irrigated with sodium hypochlorite. Final irrigation used one of four agents: 17% EDTA, 20% citric acid, 0.2% chitosan, or 5% fulvic acid, followed by sonic activation. Canals were obturated …

Study Of The Changes In Vestibular Bone Plate Absorption Values After Implant Placement Under Immediate Loading For A Period Of 5 Months Using Cbct: An In Vivo Study, Hassen Merzouk, Samir Habibi, Ben Ali Boutabout

International Arab Journal of Dentistry

Introduction: The study focuses on the success and durability of dental implants, which depends heavily on the bone surrounding them. Changes in this bone affect how the implant looks and functions. The researchers aimed to use CBCT, a modern three-dimensional imaging tool, to accurately measure changes in the thickness of the bone plate on the cheek side (vestibular bone plate) around implants placed in the lower jaw (mandible). Specifically, they looked at bone changes five months after placing implants that were immediately loaded (meaning they were used for chewing right away) in 15 patients who had missing molars or premolars. …

Effect Of Root Canal Sealer Type On Obturation Density: An In Vitro Comparison Of Ah Plus, Bioactive Rcs, Novel Bioactive Glass-Based Bioceramic Sealer, And Zinc Oxide Eugenol, Poulomi Guha, Pradeep Solete, Nishitha Arun, Praveen Kumar Elango, Sanyukta Singh

International Arab Journal of Dentistry

Objectives: To compare the obturation density of four root canal sealers—AH Plus, Bioactive RCS, a novel bioactive glass-based bioceramic sealer, and zinc oxide eugenol (ZOE) using a standardized single-cone obturation technique.

Methods: Forty extracted premolars were endodontically prepared to a 15 mm working length and divided into four groups (n=10). Each group was obturated with the same F2-sized guttapercha cones and one of the sealers being evaluated. Weight variations pre-obturation and postobturation, and after complete sealer setting were used to calculate obturation density. Data were analyzed using one-way ANOVA and Tukey’s post hoc test (p < 0.05) to test for group differences.

Results: The bioactive glass-based sealer …

New Species Of Kronosvirus Bacteriophages That Infect Caulobacter Strain Cbr1, Tannaz Mohammadi, Austin Millwood, Maherah Shaik, Bert Ely

Faculty Publications

Caulobacter segnis strain CBR1 enhances the germination rate and total biomass of Arabidopsis plants. Since bacteriophages are usually present in the rhizosphere, we isolated three additional bacteriophages, designated TMCBR2, TMCBR3, and W2, that can replicate using CBR1 as the host strain. The genome sizes and morphologies of the new phages were similar to those of the previously isolated Kronos phage, and when we compared the nucleotide sequence of these new phage genomes, we found only minor differences among the four phage genomes. Pairwise sequence comparisons indicated that the phage genomes should be grouped into three separate species within Kronosvirus genus. …

Biologically Oriented Preparation Technique In Implant-Supported Fixed Prostheses: A Systematic Review, Ghida Lawand, Roudy Khayat, Yara Ismail, Maryse Nassif, Mahmood Dashti, Jimmy Londono, Rami Al Bahri, Hani Tohme

International Arab Journal of Dentistry

This systematic review was conducted to assess the clinical and esthetic outcomes associated with convergent collar implants and compare them to divergent collar implant. The review was registered on the PROSPERO register (registration number: CRD42022326338). The systematic literature search was conducted in accordance with PRISMA guidelines to identify studies on implant-supported prostheses related to the Biologically Oriented Preparation Technique (BOPT) until June 2023. Inclusion criteria involved experimental and clinical studies published in peer-reviewed journals, excluding expert opinions. Data extraction was performed, adhering to predefined parameters for BOPT, specifications of convergent collar implants, and the assessment of outcomes. The systematic review …

Preliminary Sonification Of Enso Using Traditional Javanese Gamelan Scales, Sandy Hs Herho, Rusmawan Suwarman, Nurjanna J. Trilaksono, Iwan P. Anwar, Faiz R. Fajary

Northeast Journal of Complex Systems (NEJCS)

Sonification—the mapping of data to non-speech audio—offers an underexplored channel for representing complex dynamical systems. We treat El Niño-Southern Oscillation (ENSO), a canonical example of low-dimensional climate chaos, as a test case for culturally-situated sonification evaluated through complex systems diagnostics. Using parameter-mapping sonification of the Niño 3.4 sea surface temperature anomaly index (1870–2024), we encode ENSO variability into two traditional Javanese gamelan pentatonic systems (pelog and slendro) across four composition strategies, then analyze the resulting audio as trajectories in a two- dimensional acoustic phase space. Recurrence-based diagnostics, convex hull ge- ometry, and coupling analysis reveal that the sonification …

Impact Of One Nucleotide On Organ Enumeration And Phyllotaxy In Arabidopsis Thaliana, Hazel R. Frans, Tara Phelps-Durr

SACAD: Scholarly Activities

Arabidopsis thaliana is a model organism used by scientists to study plant genetics, development, and physiology. CRISPR-Cas9 is a biotechnology tool adapted from a bacterial defense mechanism to precisely edit DNA using a guide mRNA and a Cas9 protein. This project aims to create CRISPR-CAS9 mutations in the APETALA3 (AP3) gene of the model plant Arabidopsis thaliana. AP3 is a class B gene critical to the petal and stamen development of Arabidopsis flowers. The AP3 protein contains a MADS domain, which binds directly to DNA and may be responsible for the expression of the CaRG-box genes. AP3 works in conjunction …

Mondo: Integrating Disease Terminology Across Communities., Nicole A Vasilevsky, Sabrina Toro, Nicolas Matentzoglu, Joseph E Flack, Kathleen R Mullen, Harshad Hegde, Sarah Gehrke, Patricia L Whetzel, Yousif Shwetar, Nomi L Harris, Mee S Ngu, Gioconda L Alyea, Megan S Kane, Paola Roncaglia, Eric Sid, Courtney L Thaxton, Valerie Wood, Roshini S Abraham, Maria Isabel Achatz, Pamela Ajuyah, Joanna S Amberger, Lawrence Babb, Jasmine Baker, James P Balhoff, Jonathan S Berg, Amol Bhalla, Xavier Bofill-De Ros, Ian R Braun, Eleanor C Broeren, Blake K Byer, Alicia B Byrne, Tiffany J Callahan, Leigh Carmody, Lauren E Chan, Amanda R Clause, Julie S Cohen, Marcello Deluca, Natalie T Deuitch, May Flowers, Jamie Fraser, Toyofumi Fujiwara, Vanessa Gitau, Jennifer L Goldstein, Dylan Gration, Tudor Groza, Benjamin M Gyori, William Hankey, Jason A Hilton, Daniel S Himmelstein, Stephanie S Hong, Charles T Hoyt, Robert Huether, Eric Hurwitz, Julius O B Jacobsen, Atsuo Kikuchi, Sebastian Köhler, Daniel R Korn, David Lagorce, Bryan J Laraway, Jane Y Li, Adriana J Malheiro, James Mclaughlin, Birgit H M Meldal, Shruthi Mohan, Sierra A T Moxon, Monica C Munoz-Torres, Tristan H Nelson, Frank W Nicholas, David Ochoa, Daniel Olson, Tudor I Oprea, Tomiko T Oskotsky, David Osumi-Sutherland, Kelley Paris, Helen E Parkinson, Zoë M Pendlington, Xiao P Peng, Amy Pizzino, Sharon E Plon, Bradford C Powell, Julie C Ratliff, Heidi L Rehm, Lyubov Remennik, Erin R Riggs, Sean Roberts, Peter N Robinson, Justyne E Ross, Kevin Schaper, Brian M Schilder, Johanna L Schmidt, Elliott W Sharp, Morgan N Similuk, Damian Smedley, Tam P Sneddon, Rachel Sparks, Ray Stefancsik, Gregory S Stupp, Shilpa Sundar, Terue Takatsuki, Imke Tammen, Kezang C Tshering, Deepak R Unni, Eloise Valasek, Adeline Vanderver, Alex H Wagner, Ryan F Webb, Danielle Welter, Doron Yaya-Stupp, Andreas Zankl, Xingmin Aaron Zhang, Julie A Mcmurry, Christopher G Chute, Ada Hamosh, Christopher J Mungall, Melissa A Haendel

Faculty Research 2026

Precision medicine aims to enhance diagnosis, treatment, and prognosis by integrating multimodal data at the point of care. However, challenges arise due to the vast number of diseases, differing methods of classification, and conflicting terminological coding systems and practices used to represent molecular definitions of disease. This lack of interoperability artificially constrains the potential for diagnosis, clinical decision support, care outcome analysis, as well as data linkage across research domains to support the development or repurposing of therapeutics. There is a clear and pressing need for a unified system for managing disease entities⁠-including identifiers, synonyms, and definitions. To address these …

Regulation Of Gene Expression By Small Upstream Open Reading Frames In Escherichia Coli, Andrea R. Vansledright

Honors Capstone Projects and Theses

No abstract provided.

Design And Numerical Analysis Of A High-Quality Factor Plasmonic 1 × 3 Demultiplexer Based On Mim Waveguides, Karrar A. Kzar, Ahmed Ghanim Wadday, Sinan M. Abdulsatar

Iraqi Journal of Computers, Communications, Control and Systems Engineering

This study aims to design plasmonic filters as essential components in photonic integrated circuits,based on surface plasmon polaritons (SPPs).Surface plasmon polaritons (SPPs) are electromagnetic waves that are transmitted at metal-insulator boundaries.Optical apparatuses founded on SPPs have been widely planned too deliberate because of their outstanding possessions, like beat the diffraction limit and treatment light at subwavelength range and at high speed in minor constructions. Design and analyzing a compact 1 × 3 optical demultiplexer in a plasmonic nano structure be also designed utilize the finite element method (FEM), and the suggested structure was arithmetically simulated utilize COMSOL Multiphysics 5.6 software. …

Snail Snacks: Introduced Seaweeds Facilitate Performance Of An Introduced Marine Snail, Alysha B. Putnam, Adeline Dwyer, Paulette Peckol

Biological Sciences: Faculty Publications

Facilitative interactions among introduced species can accelerate invasion and community change, yet positive interactions are often overlooked compared to competition and predation. In marine systems, positive facilitation is more commonly demonstrated indirectly through habitat modification, with fewer studies examining direct performance impacts. While introduced herbivores in non-marine systems consume and even prefer introduced food sources, this pattern is less studied in marine environments. In this study, we tested grazing preferences and growth responses of the introduced periwinkle Littorina littorea and the native Littorina obtusata, when fed native and introduced seaweeds from southern New England. L. littorea grazed broadly across …

Morphology, Phylogeny And Distribution Of Scaeurgus (Cephalopoda: Octopoda) In Southeast Pacific Seamounts, Sergio A. Carrasco, Christian M. Ibáñez, Andrea I. Varela, Jan M. Tapia-Guerra, Erin E. Easton, Javier Sellanes

School of Earth, Environmental, & Marine Sciences Faculty Publications

Octopuses of the genus Scaeurgus inhabit upper bathyal benthic habitats and are composed of five recognized species. In this study, we conducted morphological and molecular phylogenetic analyses of two individuals of Scaeurgus and provided additional field observations of the genus in seamounts of the Nazca and Salas y Gómez ridges (southeast Pacific Ocean) over the last six years. Specifically, two specimens of Scaeurgus were collected at 234 m depth in 2019 during the EPIC oceanographic cruise onboard the R/V Mirai (JAMSTEC, Japan), and five other specimens were video recorded at depths between 210 and 340 m during three oceanographic expeditions …

Sex-Specific Differences In Lung Mitochondrial Function And Injury In Rats Exposed To Hyperoxia, Taheri Pardis, Abraham G. Taye, Devanshi D. Dave, Elizabeth R. Jacobs, Guru Prasad Sharma, Anne V. Clough, Ranjan K. Dash, Said H. Audi

Mathematical and Statistical Science Faculty Research and Publications

Hyperoxia is both an essential therapy and a contributor to lung injury in acute respiratory distress syndrome. We hypothesized that adult female rats are relatively protected from hyperoxia-induced acute lung injury (HALI) compared with males and that this protection is associated with sex-dependent differences in lung mitochondrial bioenergetics and H₂O₂ production. Adult rats were exposed to room air (normoxia) or hyperoxia (>95% O₂) for up to 60 h. Lung injury was assessed by pleural effusion, lung wet weight, pulmonary vascular filtration coefficient (K_f), histologic injury scores, and cleaved caspase-3 (CC3) staining. …

A Novel Endolysin Of Phage Persinger Infecting Pseudomonas Aeruginosa: Activity Against Biofilms And Structural Prediction, Layla Abbud

James Madison Undergraduate Research Journal (JMURJ)

Endolysins are proteins that degrade bacterial cell walls. They are encoded on the genome of bacteriophages (viruses that infect and kill bacteria). At the end of the lytic cycle of a bacteriophage, endolysins cut the peptidoglycan layer of the bacterial cell wall, allowing the release of viral progeny. Pseudomonas aeruginosa is a well-known multidrug-resistant human pathogen that causes serious problems, especially in patients diagnosed with cystic fibrosis (CF). One property of P. aeruginosa is the production of biofilms, which is a defense against antibiotics. Previous research has shown that endolysins from bacteriophages can degrade biofilms. The endolysin protein from Persinger, …

Irene Turk Rorimer, Dwight Chasar

Cleveland Bird Calendar

No abstract provided.

In Memorium: Tony Hess, Delores Cole, Paula Lozano

Cleveland Bird Calendar

No abstract provided.