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2020

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Simple Determination Of Histamine In Cheese By Capillary Electrophoresis With Diode Array Detection, E. Numanoǧlu, I.H. Boyaci, A. Topcu Jul 2020

Simple Determination Of Histamine In Cheese By Capillary Electrophoresis With Diode Array Detection, E. Numanoǧlu, I.H. Boyaci, A. Topcu

Journal of Food and Drug Analysis

A capillary electrophoresis method with diode array detection for quantitative analysis of histamine (HIS) in cheese was developed and validated. The electrophoretic conditions studied for method optimization were buffer concentration (25-100 mM) and pH (2.5-6.5), voltage (10-30 kV) and temperature (20-35°C). The effects of these conditions on peak shape, sensitivity and migration time were tested. The optimized method was validated for linearity, sensitivity, accuracy, precision (intraday and interday repeatability) and recovery. The limit of detection (LOD) and limit of quantitation (LOQ) values of the validated method were determined as 20 mg/kg and 61 mg/kg for HIS, respectively. This method was …

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Analysis Of Bioactive Triterpenes In Eriobotrya Japonica Lindl. By High-Performance Liquid Chromatography, H.-Y. Ho, W.-C. Lin, S. Kitanaka, C.-T. Chang, J.-B. Wu Jul 2020

Analysis Of Bioactive Triterpenes In Eriobotrya Japonica Lindl. By High-Performance Liquid Chromatography, H.-Y. Ho, W.-C. Lin, S. Kitanaka, C.-T. Chang, J.-B. Wu

Journal of Food and Drug Analysis

Eriobotrya japonica (Rosaceae) has been used for clearing away heat in the lung to stop cough and lowering the adverse flow of gi to stop vomiting. High-performance liquid chromatographic method was applied to assay the five triterpenes contents in E. japonica from various locations. Samples were analyzed on a HyPURITY C-18 column (5 μm, 4.6 × 250 mm) eluted at a rate of 0.5 mL/min with a solvent of A - B (A, Methanol; B, 0.15% acetic acid aqueous; A: B = 85: 15, v/v). Triterpenes were detected by refractive index detection (RID). Regression equations of the calibration curves revealed …

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The Protective Effect Of Amino Acids On The Copper(Ii)-Catalyzed Autoxidation Of Ascorbic Acid, F. Imer, E. Aldemir, H. Kiliç, I. Sonmezoǧlu, R. Apak Jul 2020

The Protective Effect Of Amino Acids On The Copper(Ii)-Catalyzed Autoxidation Of Ascorbic Acid, F. Imer, E. Aldemir, H. Kiliç, I. Sonmezoǧlu, R. Apak

Journal of Food and Drug Analysis

Protection of ascorbic acid (AA: vitamin C) from Cu(II)-catalyzed autoxidation is an important aspect of antioxidant chemistry. The autoxidation of AA in the absence and presence of Cu(II) ions was investigated in aerated solution at room temperature and I = 0.1 ionic strength (KNO3). Effects of eight biochemically important amino acids (alanine, glycine, aspartic acid, asparagine, glutamic acid, glutamine, phenylalanine, and histidine) on this system were studied. The concentration of unoxidized AA remaining in solution was measured with the CUPRAC (cupric ion reducing antioxidant capacity) spectrophotometric method without interference from the amino acids tested. The autoxidation rate constants of AA …

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Simultaneous Determination Of Eleven Quinolones Antibacterial Residues In Marine Products And Animal Tissues By Liquid Chromatography With Fluorescence Detection, C.-S. Chang, W.-H. Wang, C.-E. Tsai Jul 2020

Simultaneous Determination Of Eleven Quinolones Antibacterial Residues In Marine Products And Animal Tissues By Liquid Chromatography With Fluorescence Detection, C.-S. Chang, W.-H. Wang, C.-E. Tsai

Journal of Food and Drug Analysis

A simple and efficient multiresidue method was developed for determining 11 quinolones (QNs; marbofloxacin, norfloxacin, ciprofloxacin, lomefloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid, nalidixic acid and flumequine) in chicken, pork, fish and shrimp. The analytes were extracted with 0.3% metaphosphoric acid and acetonitrile (1:1, v/v), followed by a HLB cartridge clean-up procedure. The HPLC separation was carried out on a symmetry column C-18 (250 mm × 4.5 mm id., 5 μm) with linear gradient elution of 0.1% formic acid and acetonitrile as mobile phase and programmable fluorescence detection. The method was validated by spiking blank animals tissues at three different …

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Butyltin Compounds In Fishes Commonly Sold In Taiwan Markets, C.-H. Chen, K.-M. Huang, C.-H. Ho, C.-F. Chang, S.-M. Liu Jul 2020

Butyltin Compounds In Fishes Commonly Sold In Taiwan Markets, C.-H. Chen, K.-M. Huang, C.-H. Ho, C.-F. Chang, S.-M. Liu

Journal of Food and Drug Analysis

Fishes commonly consumed in Taiwan were purchased from four fish markets at seasonal intervals in 2006 in order to monitor the butyltin residues, including monobutyltin (MBT), dibutyltin (DBT), tributyltin (TBT), and tetrabutyltin (TeBT), in fishes caught along the west coast of Taiwan. Concentration of MBT, DBT, TBT, and TeBT in the muscle or the internal organ of these fishes were in the range of n.d.-949.2 ± 49.1, n.d.-56.2 ± 7.9, n.d.-70.8 ± 10.0, and n.d.-103.8 ± 76.9 ng/g (wet weight), respectively. Owing to the toxicological similarity between DBT and TBT, total concentrations of DBT plus TBT in the muscle or …

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Dna Protection And Antitumor Effect Of Water Extract From Residue Of Jelly Fig (Ficus Awkeotsang Makino) Achenes, Y.-W. Lin, Y.T. Wang, H.-M. Chang, J.S.-B. Wu Jul 2020

Dna Protection And Antitumor Effect Of Water Extract From Residue Of Jelly Fig (Ficus Awkeotsang Makino) Achenes, Y.-W. Lin, Y.T. Wang, H.-M. Chang, J.S.-B. Wu

Journal of Food and Drug Analysis

The present study investigated the effect of water extract from the residue of jelly fig achenes (WERJFA) on the protection of DNA against hydroxyl radical damage and on the growth inhibition of three cancer cell lines, including mouse colon cancer CT-26, human hepatoma HepG2 and breast cancer MCF-7. DNA protection against hydroxyl radical from hydrogen peroxide was assayed by agarose gel electrophoresis. Growth inhibition of the cell lines was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed that WERJFA was able to reduce the hydroxyl radical-induced DNA damage and to inhibit the growth of the three cancer cell lines …

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Development And Validation Of An Hplc Method For The Purity Assay Of Bzm, The Precursor Of Striatal Dopaminergic D2/D3 Receptor Spect Imaging Agent [123i]Ibzm (Lodobenzamide), K.-T. Liu, H.-H. Yang, Y.-C. Hsia, A.-S. Yang, C.-Y. Su, T.-S. Lin, L.-H. Shen Jul 2020

Development And Validation Of An Hplc Method For The Purity Assay Of Bzm, The Precursor Of Striatal Dopaminergic D2/D3 Receptor Spect Imaging Agent [123i]Ibzm (Lodobenzamide), K.-T. Liu, H.-H. Yang, Y.-C. Hsia, A.-S. Yang, C.-Y. Su, T.-S. Lin, L.-H. Shen

Journal of Food and Drug Analysis

We developed and validated a reverse-phase high performance liquid chromatography (RP-HPLC) method for the purity assay of BZM. BZM is a precursor and free ligand of [123I]IBZM (iodobenzamide) that is a SPECT imaging agent for striatal dopaminergic D2/D3 receptor (D2R). The chromatographic separation was achieved on a Zorbax Eclipse XDB-C18 column with a gradient mobile phase consisting of 10 mM ammonium acetate buffer, pH 7.0 and acetonitrile at a flow rate of 0.5 mL/min. The absorbance at 254 nm against the concentration of BZM over the range 0.5-5.5. μg was linear, with a correlation coefficient above 0.9997. The limit of …

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Simultaneous Kinetic-Potentiometric Determination Of Levodopa And Carbidopa Using Multivariate Calibration Methods, Karimi M. Ali, M.H. Mashhadizadeh, Ardakani M. Mazloum, N. Sahraei Jul 2020

Simultaneous Kinetic-Potentiometric Determination Of Levodopa And Carbidopa Using Multivariate Calibration Methods, Karimi M. Ali, M.H. Mashhadizadeh, Ardakani M. Mazloum, N. Sahraei

Journal of Food and Drug Analysis

Partial least squares (PLS) and principle component regression (PCR) multivariate calibration methods were applied to the simultaneous determination of carbidopa and levodopa using kinetic data from novel potentiometry methods. These methods were based on the rate of chloride ion production in the reaction of carbidopa and levodopa with N-chlorosuccinimide (NCS) which was monitored by a chloride ion-selective electrode. The experimental data shows suitability of ion-selective electrodes (ISEs) to be used as detectors not only for the direct determination of chloride ion but also for simultaneous kinetic-potentiometric analysis using chemometric methods. These methods are based on the differences observed in the …

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Content Of Total Phenolics And Anthocyanins And The Antimutagenicity Of Aspergillus Awamori-Fermented Black Soybean After Heat Treatment, Y.-J. Wang, C.-C. Chou Jul 2020

Content Of Total Phenolics And Anthocyanins And The Antimutagenicity Of Aspergillus Awamori-Fermented Black Soybean After Heat Treatment, Y.-J. Wang, C.-C. Chou

Journal of Food and Drug Analysis

The Aspergillus awamoir-fermented black soybean, possessing enhanced functional properties, was previously suggested to be a potentially functional ingredient in the formation of healthy foods. In this study, the effect of heat treatments (40-100°C for 30 min) on the changes of total phenolics and anthocyanin contents, mutagenicity and the antimutagenicity of fermented black soybeans against 4-nitroquinoline-N-oxide, a direct mutagen, and benzo[a]pyrene, an indirect mutagen, on Salmonella Typhimurium TA100 and TA98 were examined. Results revealed that the heated-fermented black soybean showed no mutagenicity. Heating the fermented black soybean at 80 or 100°C for 30 min resulted in a reduced antimutagenicity with the …

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Establishment Of A National Taiwan Standard And Working Reagent For Human Parvovirus B19 Dna Nucleic Acid Amplification Techniques (Nat), Y.-C. Yang, C.-J. Chang, C.-I. Tseng, D.-Y. Wang, H.-F. Cheng, C.-P. Lin Jul 2020

Establishment Of A National Taiwan Standard And Working Reagent For Human Parvovirus B19 Dna Nucleic Acid Amplification Techniques (Nat), Y.-C. Yang, C.-J. Chang, C.-I. Tseng, D.-Y. Wang, H.-F. Cheng, C.-P. Lin

Journal of Food and Drug Analysis

Mandatory requirements for plasma pools containing a maximum concentration of human parvovirus B19 DNA will be established in Taiwan in the near future. To facilitate the implementation of the policy, a National Standard and working reagent were established for human parvovirus B19 DNA nucleic acid amplification techniques (NAT). The standards are intended to be used for the quality control of B19 NAT assays and as a quantitative reference material for B19 NAT testing. A collaborative study, including 10 laboratories from seven countries, was done in order to establish the National Standard and working reagent for B19 DNA NAT assays by …

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Classification Of Fangchi Radix Samples By Multivariate Analysis, C.-C. Chuang, C.-H. Su, W.-Y. Huang, S.-J. Sheu Jul 2020

Classification Of Fangchi Radix Samples By Multivariate Analysis, C.-C. Chuang, C.-H. Su, W.-Y. Huang, S.-J. Sheu

Journal of Food and Drug Analysis

A total of 37 commercial samples of Fangchi Radix, including Sinomenium acutum, Stephania tetrandra, Aristolochia fangchi, and Cocculus orbiculatus, were collected from herb markets in Taiwan and China. The contents of 18 constituents in these samples were analyzed by high-performance liquid chromatography and were used to assess the potential relationships with their plant origins. A multivariate analysis, including a principal component analysis (PCA), cluster analysis (CA), and linear discriminant analysis (LDA) were used as the classification strategies. The successful natural groupings of the samples into 4 sets, namely, 5 S. acutum, 11 S. tetrandra, 15 A. fangchi, and 6 C. …

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A Dose-Dependent Pharmacokinetic Study Of Levodopa By Intramuscular Administration In Rabbits, L.-H. Wang, K.-Y. Hsu, F.-L. Hsu, S.-J. Lin Jul 2020

A Dose-Dependent Pharmacokinetic Study Of Levodopa By Intramuscular Administration In Rabbits, L.-H. Wang, K.-Y. Hsu, F.-L. Hsu, S.-J. Lin

Journal of Food and Drug Analysis

The dose-dependent pharmacokinetics of levodopa (L-dopa) was studied in rabbits by intramuscular administration. Three different doses of L-dopa/ carbidopa (2/0.5, 5/1.25, and 10/2.5 mg/kg) were administered to six male rabbits via an intramuscular (IM) route, and one dose of L-dopa/ carbidopa (2/0.5 mg/kg) was administered via an intravenous (IV) route with a washout period of 1-week between different doses. Plasma samples were collected after each treatment and the concentrations of L-dopa and 3-O-methyldopa (an L-dopa metabolite, 3-OMD) were measured by a sensitive high-performance liquid chromatographic (HPLC) method. Subsequently, these measurements were used to determine the pharmacokinetic behavior of L-dopa and …

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Induction Of Permeability And Apoptosis In Colon Cancer Cell Line With Chitosan, Z. Hossain, K. Takahashi Jul 2020

Induction Of Permeability And Apoptosis In Colon Cancer Cell Line With Chitosan, Z. Hossain, K. Takahashi

Journal of Food and Drug Analysis

The effect of chitosan on tight junctions (TJs) permeability on Caco-2 cell monolayer intestinal model was investigated. We have also investigated the effects of low molecular weight water-soluble chitosan (LMWC) on apoptosis in HT-29 cell. The changes in barrier properties of Caco-2 cell monolayers, including transepithelial electrical resistance (TEER) and permeability to lucifer yellow, were assessed in response to chitosan treatment. High molecular weight chitosan (HMWC) was found to cause up to 50% dose-dependent reduction in TEER of Caco-2 cell monolayer without damage to the cell membrane under lower concentration. The effect of HMWC on TJs was confirmed by increased …

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Effect Of Taurine On Toxicity Of Oxidized Cholesterol And Oxidized Fish Oil In Rats, Y.-H. Yeh, M.-H. Chen, Y.-T. Lee, H.-S. Hsieh, D.-F. Hwang Jul 2020

Effect Of Taurine On Toxicity Of Oxidized Cholesterol And Oxidized Fish Oil In Rats, Y.-H. Yeh, M.-H. Chen, Y.-T. Lee, H.-S. Hsieh, D.-F. Hwang

Journal of Food and Drug Analysis

Taurine (2-aminoethanesulfonic acid) occurs naturally in food, especially in seafood and meat. The aim of our investigation was to evaluate the effect of dietary taurine on oxidized cholesterol and oxidized fish oil induced toxicity in male Wistar rats. Thirty male wistar rats were fed with diets supplemented with 5% taurine, 2% oxidized cholesterol or 3% oxidized fish oil for 6 weeks. After feeding such diet, taurine could increase the high density lipoprotein-cholesterol (HDL-C) level in plasma, and glutathione (GSH) level in plasma, and decrease the activities of alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST) in plasma, and the …

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Molecular Recognition And Identification Of Sulfapyridine And Sulfadimethoxine By High Performance Liquid Chromatography With Molecularly Imprinted Polymer As The Stationary Phase, C.-Y. Hung, Y.-T. Huang, C.-C. Hwang Jul 2020

Molecular Recognition And Identification Of Sulfapyridine And Sulfadimethoxine By High Performance Liquid Chromatography With Molecularly Imprinted Polymer As The Stationary Phase, C.-Y. Hung, Y.-T. Huang, C.-C. Hwang

Journal of Food and Drug Analysis

Molecular imprinting technique is becoming an important tool for determining sulfonamides because of its simplicity and high separation efficiency. The creation of molecularly imprinted polymers (MIPs) for recognition of sulfapyridine and sulfadimethoxine is reported. Polymers were prepared using methacrylic acid (MAA) as the functional monomer and sulfapyridine as the template during imprinting. The recognition properties of the polymers were assessed by high performance liquid chromatography (HPLC) for their ability to retain the templates and analogous analytes. Retention time of sulfapyridine and sulfadimethoxine was approximately 8.24 and 3.89 min, respectively. In order to compare the chromatographic data from the stationary phase, …

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Amaranthus Spinosus L. Inhibits Spontaneous And Dexamethasone-Induced Apoptosis In Murine Primary Splenocytes, J.-Y. Lin, C.-Y. Li, B.-F. Lin Jul 2020

Amaranthus Spinosus L. Inhibits Spontaneous And Dexamethasone-Induced Apoptosis In Murine Primary Splenocytes, J.-Y. Lin, C.-Y. Li, B.-F. Lin

Journal of Food and Drug Analysis

Amaranthus spinosus L. (thorny amaranth) is traditionally selected to be an anti-diabetic herb in Taiwan. This study assessed the immuno-modulatory effects of wild A. spinosus water extract (WASWE) on spontaneous and dexamethasone (DEX)-induced apoptosis in murine primary splenocytes. The cultured splenocytes treated with WASWE products were harvested and analyzed to assess their apoptotic status according to DNA fragmentation by flow cytometry and agarose gel electrophoresis. The results showed WASWE inhibited the spontaneous and DEX-induced apoptosis of splenocytes. Furthermore, both WASWE and its activated charcoal-treated sample administered at concentration of 1.250 mg/mL inhibited intracellular protein levels of p53 (an apoptotic inducer …

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Arsenic Speciation In Fish On The Market, H.-T. Lin, S.-W. Chen, C.-J. Shen, C. Chu Jul 2020

Arsenic Speciation In Fish On The Market, H.-T. Lin, S.-W. Chen, C.-J. Shen, C. Chu

Journal of Food and Drug Analysis

Arsenic speciation in fish samples on the market was analyzed using gradient anion exchange high performance liquid chromatography (HPLC) with inductively coupled plasma mass spectrometry (ICPMS) detection. Freeze-dried samples were extracted by methanol and water mixture (8:2 v/ v) with a high-speed Soxhlet system; the concentration of arsenobetaine (AsB), dimethylarsinic acid (DMAA), monomethylarsonic acid (MMAA), arsenate (As(V)) and arsenite (As(III)) in the extract were then determined using HPLC-ICPMS. The method for determining arsenobetaine concentration was confirmed using standard reference material (SRM) of BCR 627 (tuna fish) and DORM-2 (dogfish muscle); recovery rates were 95 and 90%, respectively. The arsenic compounds …

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In Vitro Activation Of Peroxisome Proliferator Activated Receptor Α By Some Extracts From Food Materials, C.-Y. Chao, C.-J. Huang Jul 2020

In Vitro Activation Of Peroxisome Proliferator Activated Receptor Α By Some Extracts From Food Materials, C.-Y. Chao, C.-J. Huang

Journal of Food and Drug Analysis

Peroxisome proliferator activated receptor α (PPARα) is a ligand-dependent transcription factor that regulates the expression of genes involved in lipid metabolism and transport. Activators of PPARα may be potential hypolipidemic agents, like the fibrate-type drugs. This study was therefore aimed at identifying potential hypolipidemic functional foods by screening activators of PPARα from food materials. CHO-K1 cells were stably transfected with vectors (pBKCMV) carrying cDNAs of a (UAS)4-tk-alkaline phosphatase reporter and a chimeric receptor Gal4-PPARα LBD successively. Clones of cells responsive to Wy-14643, a known PPARα activator were selected, characterized and used for testing the PPARα activating potency of fractions extracted …

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Development Of A Multiplex Pcr Method For The Detection Of Six Common Foodborne Pathogens, I.-F. Lei, P. Roffey, C. Blanchard, K. Gu Jul 2020

Development Of A Multiplex Pcr Method For The Detection Of Six Common Foodborne Pathogens, I.-F. Lei, P. Roffey, C. Blanchard, K. Gu

Journal of Food and Drug Analysis

This study developed a multiplex PCR method for the screening and detection of six common foodborne pathogens in Macao. The m-PCR procedure, which uses six pairs of primers, produced specific amplicons of the expected sizes from mixed populations of reference bacterial strains in food samples and from pure cultures. The verocytotoxin (stx) gene of Escherichia coli 0157: H7, the hemolysin (hly) gene of Listeria monocytogenes, the invasion (invA) gene of Salmonella spp., the cholera toxin (ctx) gene of Vibrio cholerae, the thermolabile hemolysin (tlh) gene of V. parahaemolyticus, and the thermostable nuclease (nuc) gene of Staphylococcus aureus were used as …

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Factors Affecting Pge2 Production In Seaweed Gracilaria Tenuistipitata, B.-Y. Hsu, C.-Y. Tsao, T.-K. Chiou, D.-F. Hwang Jul 2020

Factors Affecting Pge2 Production In Seaweed Gracilaria Tenuistipitata, B.-Y. Hsu, C.-Y. Tsao, T.-K. Chiou, D.-F. Hwang

Journal of Food and Drug Analysis

Gracilaria tenuistipitata is an edible red alga and can be utilized as feed for small abalone and foodstuff (ex. agar extract). It is the major species of Gracilaria that is commonly cultivated and consumed in Taiwan. Effects of environmental factors on prostaglandin E2 (PGE2) production in the seaweed G. tenuistipitata were investigated in this study. PGE2 amount increased nearly 2-fold and 47% when the seaweed was exposed to two ways of fluctuation of seawater salinity from 2% to 1% and 3% for 12 hr, respectively. The stresses of low temperature and irradiance promoted the seaweed to produce PGE2. When the …

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Chemical Constituents And Anti-Platelet Aggregation Activity From The Root Of Peucedanum Formosanum, Y.-C. Chen, P.-Y. Chen, C.-C. Wu, I.-L. Tsai, I.-S. Chen Jul 2020

Chemical Constituents And Anti-Platelet Aggregation Activity From The Root Of Peucedanum Formosanum, Y.-C. Chen, P.-Y. Chen, C.-C. Wu, I.-L. Tsai, I.-S. Chen

Journal of Food and Drug Analysis

Analysis of the root extract of Peucedanum formosanum (Taiwan Qian-Hu) led to the isolation of 32 known compounds. The structures of these isolates were determined by spectral data. Some of them displayed strong anti-platelet aggregation activities. Analysis showed that most of the constituents found in P. formosanum were the same as those found in P. praeruptorum (Bai-Hua Qian-Hu), in that many isolates of both plants' roots belong to seselin-type dihydropyranocoumarins and psoralen-type furanocoumarins.

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Pharmacodynamic Evaluation Of Terminalia Bellerica For Its Antihypertensive Effect, A.-U. Khan, A.H. Gilani Jul 2020

Pharmacodynamic Evaluation Of Terminalia Bellerica For Its Antihypertensive Effect, A.-U. Khan, A.H. Gilani

Journal of Food and Drug Analysis

Terminalia bellerica has been used as a folk medicine in a variety of ailments including hypertension. Our aim was to investigate the possible mechanism of its blood pressure (BP)-lowering effect. The crude extract of Terminalia bellerica fruit (Tb.Cr) which tested positive for flavonoids, sterols and tannins induced a dose-dependent (10-100 mg/kg) fall in the arterial BP of rats under anaesthesia. In isolated guinea-pig atria, Tb.Cr inhibited the force and rate of atrial contractions. In rabbit thoracic aorta, Tb.Cr relaxed the phenylephrine (PE, 1 μM) and K+ (80 mM)-induced contractions as well as suppressed the PE (1 μM) control peaks in …

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Antibacterial Activity And Antioxidant Properties Of Water Extract From The Residue Of Jelly Fig (Ficus Awkeotsang Makino) Achenes, Y.-J. Lai, J.-N. Chen, J.S.-B. Wu Jul 2020

Antibacterial Activity And Antioxidant Properties Of Water Extract From The Residue Of Jelly Fig (Ficus Awkeotsang Makino) Achenes, Y.-J. Lai, J.-N. Chen, J.S.-B. Wu

Journal of Food and Drug Analysis

The water extract from the residue of jelly fig (Ficus awkeotsang Makino) achenes (WERJFA) was found to inhibit the growth of Bacillus cereus and Escherichia coil O157:H7 effectively. The antioxidant activity of WERJFA was evaluated in radical scavenging activity, reducing capacity, ferrous ions chelating activity, total antioxidant activity, and hydrogen peroxide scavenging activity. The reducing capacity of WERJFA at 150 μg/mL and higher was found to be better than those of α-tocopherol and BHA at the same concentration. The ferrous ion chelating activity of WERJFA was higher than that of citric acid. The antioxidant activity, the superoxide anions radical scavenging …

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Application Of Column Solid Phase Extraction Of Chromium For Indirect Determination Of Ascorbic Acid By Flame Atomic Absorption Spectrometry, A.R. Tüurker, O. Yalçinkaya, A. Tunçeli Jul 2020

Application Of Column Solid Phase Extraction Of Chromium For Indirect Determination Of Ascorbic Acid By Flame Atomic Absorption Spectrometry, A.R. Tüurker, O. Yalçinkaya, A. Tunçeli

Journal of Food and Drug Analysis

An indirect method for the determination of ascorbic acid in fruit juice and vitamin C tablets by flame atomic absorption specrometry was described. This method is based on the reduction of chromium (VI) to chromium(III) with the reducing action of ascorbic acid, separation of unreacted Cr(VI) as its 1,5-diphenilcarbazide complex on a column filled with Amberlite XAD-16, elution of the complex by 10 mL of 0.05 mol L-1 H2SO4 in methanol, and determination by flame atomic absorption spectrometry. Amount of the ascorbic acid was calculated from the amount of Cr(VI) reacted with ascorbic acid. The optimum conditions for the determination …

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A Simple And Fast Method For Arginine Determination In Grape Juice, H. Li, X. Liang, L. Feng, Y. Liu, H. Wang Jul 2020

A Simple And Fast Method For Arginine Determination In Grape Juice, H. Li, X. Liang, L. Feng, Y. Liu, H. Wang

Journal of Food and Drug Analysis

Arginine in grape juice can be metabolized by wine yeasts and malolactic bacteria to precursors of ethyl carbamate, a known carcinogen. The aim of this study was to develop a simple, fast and accurate method for determining arginine in grape juice with Sakaguchi reaction following the separation of arginine with Amberlite IR120 strong cation-exchange resin. Measurement was done under optimized conditions as follows: sample (mg) to cation-exchange resin (g) ratio of 0.683, sample pH 2.0, sample flow rate 0.5 mL/min, use of distilled water rinsing to avoid interference of other compounds adsorbed on the resin, and elution with 1 mol/L …

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Effect Of Taurine On Toxicity Of Oxidized Cholesterol In Rats, Y.-H. Yeh, M.-H. Chen, Y.-T. Lee, H.-S. Hsieh, D.-F. Hwang Jul 2020

Effect Of Taurine On Toxicity Of Oxidized Cholesterol In Rats, Y.-H. Yeh, M.-H. Chen, Y.-T. Lee, H.-S. Hsieh, D.-F. Hwang

Journal of Food and Drug Analysis

This research was aimed to study the effect of dietary taurine on the toxicity of oxidized cholesterol in male Wistar rats. The rats were divided into eight groups and fed different diets with or without supplement of 5% taurine and 3% oxidized cholesterol for 8 weeks. To evaluate effects of taurine at the same time, before diets and after diets in the food, after feeding diet with 3% oxidized cholesterol and 5% taurine at the same time, taurine could improve the decrease of body weight and the glutathione (GSH) level in the liver, and the increase of relative ratio of …

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Rapid And Specific Detection Of Enterotoxigenic Escherichia Coli And Salmonella Strains By Multiplex Pcr Systems, S.-J. Wang Jul 2020

Rapid And Specific Detection Of Enterotoxigenic Escherichia Coli And Salmonella Strains By Multiplex Pcr Systems, S.-J. Wang

Journal of Food and Drug Analysis

Escherichia coli and Salmonella are two of the most important food-borne bacterial pathogens. Classical identification for these strains is laborious, time-consuming, and may generate erroneous results. The purpose of this study was to develop a rapid and specific multiplex PCR (m-PCR) method to simultaneously detect heat labile enterotoxin gene of E. coli (LT ETEC) and oriC of Salmonella sp. Multiplex PCR using two pairs of primers produced specific amplicons of expected sizes of 163 bp and 425 bp from mixed populations of Salmonella sp. and LT ETEC bacterial strains, respectively. These primers were then used for the detection of food …

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Spectrophotometric And Atomic Absorption Spectrometric Determination Of Cephalexin And Cephradine In Dosage Forms, S.M. Al-Ghannam Jul 2020

Spectrophotometric And Atomic Absorption Spectrometric Determination Of Cephalexin And Cephradine In Dosage Forms, S.M. Al-Ghannam

Journal of Food and Drug Analysis

This paper describes two simple, sensitive and selective spectrophotometric and atomic absorption spectrometric (AAS) procedures for the determination of two compounds of cephalosporins (Cephalexin monohydrate and Cephradine). These procedures are based on the formation of ion-pair complexes between the drugs and ammonium reineckate. The formed precipitates are quantitatively determined either spectrophotometrically or by AAS procedures. The methods consist of reacting drugs with Reinecke's salt in an acidic medium at a temperature of 25 ± 2°C. The spectrophotometric procedure (procedure I) is based on dissolving the formed precipitate with acetone. The volume was completed quantitatively and absorbance of the solution was …

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Novel Spectrophotometric Method For Rapid Quantifying Acetaminophen Concentration In Emergent Situation, J.-F. Chiou, S.-L. Chen, S.-M. Chen, S.-S. Tsou, C.-Y. Wu, J.-S. Chu, T.-Z. Liu Jul 2020

Novel Spectrophotometric Method For Rapid Quantifying Acetaminophen Concentration In Emergent Situation, J.-F. Chiou, S.-L. Chen, S.-M. Chen, S.-S. Tsou, C.-Y. Wu, J.-S. Chu, T.-Z. Liu

Journal of Food and Drug Analysis

A novel spectrophotometric method for rapid quantification of acetaminophen in serum that is particularly suited for emergency usage is described. Free unconjugated acetaminophen is separated from other endogenous interferents by extracting the drug into ethyl acetate by simply using a vortex mixer. Subsequently, the cupric ions in the reagent are reduced by the phenolic hydroxyl groups present in the drug. The resultant cuprous ions then interacted with bicinchoninic acid (BCA) to form a chromophore which absorbs maximally at 562 nm. The proposed method has a linearity range from 50 to 400 μg/mL. The method was precise with day-to-day coefficients of …

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Natural Products With Skin - Whitening Effects, J.-W. Lin, H.-M. Chiang, Y.-C. Lin, K.-C. Wen Jul 2020

Natural Products With Skin - Whitening Effects, J.-W. Lin, H.-M. Chiang, Y.-C. Lin, K.-C. Wen

Journal of Food and Drug Analysis

UV radiation is widely considered as a major cause of skin pigmentation. Upon exposure to UV radiation, the melanocytes increase the production of intracellular nitric oxide, which triggers signal transduction cascades to initiate melanogenesis by tyrosinase. UV radiation also influences melanogenesis through a paracrine regulation process involving the keratinocytes. Although a number of hypopigmenting products have been developed, those from natural sources are preferred and will predominate in the cosmetics market. More active compounds such as phenols, flavonoids, coumarins and other derivatives have been identified from natural sources. This article summarized natural skin whitening products involving tyrosinase blockers like phenols …

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