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Columbus State University Honors College: Senior Theses, Fall 2020/Spring 2021, Abigail Abernathy, Micah Arnholt, Joshua Butler, Ashlyn Dewberry, Vivian Duncan, Shannon Eshman, Macy Frazier, Bailey Gaines, Meenal Joshi, Brenna Kincaid, Taylor Lewis, Molli Mcginnis, Elijah Neundorfer, Erin Perry, Grace Pippas, Elise Rhinebolt, Caitlin Schwartz, Tatseyana Taylor, Lyndi Whetzel

Senior Theses from the Honors College

This is a collection of senior theses written by honors students at Columbus State University during the Fall 2020 and Spring 2021 semesters.

Titles:

- "Comparison of Histological Techniques to Determine Intersex Prevalence and Severity in Testes of Largemouth Bass *Micropterus salmoides) and Spotted Bass (Micropterus punctulatus) From the Chattahoochee River, U.S.A" , by Abigail Abernathy

- "The Applications of Space Syntax Theory and Depthmap Technology to Problems of Race and Income Inequality in Midtown, Columbus ,GA" , by Micah Arnholt

- "Utilizing the Dalcroze Method in the Secondary Choral Classroom" , by Joshua Butler

- "A Tale of Two …

Projecting Regions Of North Atlantic Right Whale, Eubalaena Glacialis, Habitat Suitability In The Gulf Of Maine In 2050, Camille Ross

Honors Theses

North Atlantic right whales (Eubalaena glacialis) are endangered. Understanding the role environmental conditions play in habitat suitability is key to determining the regions in need of protection for conservation of the species, particularly as climate change shifts suitable habitat. This thesis uses three species distribution modeling algorithms, together with historical data on whale abundance(1993 to 2009) and environmental covariates to build monthly ensemble models of past E. glacialis habitat suitability in the Gulf of Maine. Then, the models are projected onto the year 2050 for a range of climate scenarios. Specifically, the distribution of the species was modeled …

The “Humanitarian Mystique:” Tracing The Rhetoric And Politics Of Aid In Southeast Asia From The Age Of The Civilizing Mission To The Present, Elizabeth M. Holland

Honors Theses

In contemporary Western popular culture, humanitarian action often serves as the ultimate expression of altruism, compassion, and moral obligation. This research historicizes humanitarianism to understand the assumptions that underlie its affective appeal. Oversimplified narratives of aid work frequently fail to acknowledge the historical and geopolitical context in which this work occurs. I argue that humanitarianism, as both a discursive tool and code of practice, makes visible some legacies of the ‘civilizing mission’ – the ideology used to justify colonialism in the late nineteenth and early twentieth centuries. An exercise in comparative history, this research consists of three spatially and temporally …

Propaganda Y Progreso: El Rol Paradójico De La Magia En La España Premoderna, Alexander K. Rhodes

Honors Theses

La magia tiene varios roles diferentes en la literatura española premoderna. A causa de las connotaciones pecaminosas de la magia, frecuentemente se usa para discriminar contra un grupo subalterno, como en la obra de teatro El niño inocente de La Guardia de Lope de Vega. Se ve que esta forma de discriminación difunde y apoya la anxiety of sameness definida por Christina Lee. La anxiety of sameness es el temor de los nobles de ser infiltrados por los no nobles, incluyendo a los judíos, los moriscos y los de la clase baja. Pero, como se ve en esta tesis, la …

Development And Validation Of Gene Delivery Methods For ​Crassostrea Virginica, Adrienne N. Tracy

Honors Theses

The Eastern oyster (Crassostrea virginica) is an important part of the East Coastal USA economy because aquaculture creates jobs. Sadly, the oysters are under constant threat due to increasing pollution, red tides, and diseases. Bivalves, and oysters in particular, are also becoming potential model organisms in medical research. With the sequencing of the oyster genome, scientists are focusing on deciphering the function of the predicted genes. However, the limited number of molecular and cellular tools available makes functional annotation of the genome challenging. A consistent, replicable gene delivery system needs to be developed to assess gene function and understand the …

Impacts Of A Movie's Rivals' Releasing Decisions On Its Domestic Box Office Revenue, Yuanyuan Dong

Honors Theses

This paper uses the OLS regression model to investigate and quantify the competition effects in the film industry, aiming to help film distributors in the making releasing decision. To do so, I define the movies that release within the same week as rivals and use the rivals’ characteristics variables to capture the competition effects. The regression results show that a movie’s rivals’ total production budgets and the number of opening weekend’s theater have significantly negative impacts on this movie’s box office revenue. After adding control variables and indicator variables for seasonality, the competition effects become less obvious with less precision. …

"They Shall Be A Kosmos:" Alexander Von Humboldt And The Ecopoetics Of Walt Whitman, Benjamin Theyerl

Honors Theses

Places the naturalist Alexander Von Humboldt's proto-ecological ideas in conversation with Walt Whitman's poetry to show how the poet developed an ecopoetics in conversation with the natural sciences of his time, with specific attention to Von Humboldt's theory of the "kosmos" - by which Whitman's poetic persona self-identified. These recognitions are combined with how Whitman's idealized version of the American poet as a “kosmos” creates a political ecology in Whitman’s work, placing his ecopoetics into environmental discourses that resonate from their origin in the nineteenth century to our present ecological moment today.

Physical Property Improvement Of Alginate Capsule By Gellan Gum, Bacterial Cellulose And Chitosan, Aungsana Prajaksangsiri

Chulalongkorn University Theses and Dissertations (Chula ETD)

The kaffir lime oil (KLO) loaded alginate macrocapsules were prepared via a coacervation method using a coaxial glass-tube. The aims of this research were to improve the mechanical property of alginate capsule shell and to study KLO release profiles which altered by those additives. The addition of gellan gum and 2,2,6,6-tetramethylpiperidine-1-oxy radical (TEMPO)-oxidized bacterial cellulose (TOBC) in the shell-forming solution can improve the flexibility and compression strength of capsules. The size and shell thickness of capsules were in a range of 3-4 mm and 54-91 µm, respectively. Besides, the increase of TOBC amount achieved the smoother surface but increased the …

Factors Influencing The Acceptance For Adoption Of Innovative Medical Self-Test, Sumontida Sayachak

Chulalongkorn University Theses and Dissertations (Chula ETD)

There are multiple barriers of health self-care. Self-testing and information technology would empower self-care and medical industry. In addition, it may reduce the country’s public health burden cost. However, there is very limited research on IT related to health care and customer belief in Thailand. This study, alternate innovative mixed models in software was developed to understand individual’s characteristics and influencing factors for the adoption of medical self-testing. A cross-sectional survey was conducted using self-administrated questionnaires that constructed based on Health belief model (HBM), Extending the unified theory of acceptance and use of technology (UTAUT2) including related significant psychological determinants. …

Chemosensitizing Effect Of Streptomyces Crude Extract On Cisplatin-Induced Apoptosis In Human Lung Cancer H460 Cells, Theint Myat Noe Paing

Chulalongkorn University Theses and Dissertations (Chula ETD)

Although cisplatin has been considered as a first-line chemotherapeutic drug for the treatment strategy of lung cancer, its curative efficacy is limited by the occurrence of drug resistance and adverse effects. The application of non-toxic natural products for sensitization of cancer cells to conventional drugs is a recent novel strategy for cancer management to tackle these problems. This study aims to investigate effective chemosensitizer produced by Streptomyces sp. on the growth inhibition of human H460 lung cancer cells. Herein, three of eight strains isolated from peat swamp forest, Yala Province, Thailand which showed good purity analyzed by 16S rRNA sequencing …

โครงการวิจัยแนวทางการแปลถ้อยคำที่สะท้อนอุปลักษณ์เชิงมโนทัศน์ในตัวบทโฆษณาผลิตภัณฑ์เครื่องสำอางบนเว็บไซต์จากภาษาอังกฤษเป็นภาษาไทย, อรสุรางค์ แสงสมสุรศักดิ์

Chulalongkorn University Theses and Dissertations (Chula ETD)

สารนิพนธ์ฉบับนี้มุ่งศึกษาอุปลักษณ์เชิงมโนทัศน์ในตัวบทโฆษณาผลิตภัณฑ์เครื่องสำอาง รวมถึงศึกษากลวิธีการถ่ายทอดอุปลักษณ์เชิงมโนทัศน์จากต้นฉบับไปยังฉบับแปล การวิจัยนี้รวบรวมข้อมูลผลิตภัณฑ์เครื่องสำอางในกลุ่มผลิตภัณฑ์บำรุงผิวและเครื่องสำอางสำหรับตกแต่งทั้งหมด 240 ผลิตภัณฑ์จากเว็บไซต์ตราสินค้าที่มีทั้งภาษาไทยและภาษาอังกฤษ ผู้วิจัยศึกษาแนวคิดอุปลักษณ์เชิงมโนทัศน์ตามแนวภาษาศาสตร์ปริชานของ Lakoff และ Johnson (2003) แนวทางการระบุรูปภาษาแสดงอุปลักษณ์จากกลุ่มนักวิชาการ Pragglejaz Group (2007) และการจัดประเภทกลุ่มคำศัพท์ตามวงความหมายจากงานวิจัยเกี่ยวกับอุปลักษณ์เชิงมโนทัศน์ในตัวบทประเภทต่าง ๆ รวมถึงการศึกษาลักษณะภาษาโฆษณาและประเภทอุปลักษณ์ที่พบในโฆษณาเครื่องสำอางในภาษาอังกฤษและภาษาไทย และจากการศึกษาแนวทางการแปลอุปลักษณ์ตามแนวคิดแบบดั้งเดิมและตามแนวภาษาศาสตร์ปริชาน ผู้วิจัยได้เลือกใช้กรอบการวิเคราะห์กลวิธีการแปลที่ผสมผสานกันของ Deignan et al. (1997) Schäffner (2004) และ Toury (1995) ผลการวิจัยพบว่า ในตัวบทโฆษณาเครื่องสำอางภาษาอังกฤษที่คัดเลือกมามีอุปลักษณ์ทั้งหมด 29 ประเภท มีจำนวนรูปภาษาที่แสดงอุปลักษณ์ทั้งหมด 1,391 ตัวอย่าง ผู้วิจัยเลือกอุปลักษณ์ที่พบสูงสุดใน 10 อันดับแรก ได้แก่ อุปลักษณ์สงคราม แสง มนุษย์ สุขภาพ พืชและการเพาะปลูก สิ่งทอและเครื่องนุ่งห่ม เครื่องจักร ศิลปะ อาหารและโภชนาการ และภาชนะ ซึ่งจำนวนรูปภาษาที่แสดงอุปลักษณ์ทั้งหมด 1,166 ตัวอย่าง นำมาวิเคราะห์อุปลักษณ์ที่พบในฉบับแปลและกลวิธีการแปล พบว่าภาษาไทยและภาษาอังกฤษมีอุปลักษณ์ทั้งหมดนี้เหมือนกัน ผลการวิเคราะห์กลวิธีการแปลเป็นไปตามสมมติฐานที่ตั้งไว้ กล่าวคือ พบว่ากลวิธีการแปลที่พบมากที่สุดคือ 1) การแปลโดยรักษามโนทัศน์เดิม แต่รูปภาษาแสดงอุปลักษณ์ต่างจากต้นฉบับ ร้อยละ 51.41 ตามด้วย 2) การแปลโดยรักษามโนทัศน์เดิม และรูปภาษาแสดงอุปลักษณ์ตรงตามต้นฉบับ ร้อยละ 26.90 3) การแปลโดยไม่รักษามโนทัศน์ (ไม่มีรูปภาษาแสดงอุปลักษณ์) ร้อยละ 12.93 4) การแปลโดยใช้มโนทัศน์ที่แตกต่างกัน ร้อยละ 4.62 และ 5) การละไม่แปล ร้อยละ 4.11 นอกจากนี้ ยังพบว่ามีการเพิ่มมโนทัศน์ที่ไม่ปรากฏในต้นฉบับเข้ามาในฉบับแปลด้วย โดยสรุป การถ่ายทอดอุปลักษณ์เชิง มโนทัศน์ในตัวบทโฆษณาเครื่องสำอางจากภาษาอังกฤษเป็นภาษาไทแทบไม่พบปัญหาในการแปลที่มาจากปัจจัยทางวัฒนธรรมซึ่งอาจเป็นเพราะวัฒนธรรมปลายทางรับค่านิยมและวัฒนธรรมจากต่างชาติโดยเฉพาะเรื่องความงามมาอย่างยาวนาน จึงสามารถรักษามโนทัศน์เดิมในต้นฉบับได้เป็นส่วนใหญ่ ส่วนกลวิธีการแปลอื่น ๆ แสดงให้เห็นการปรับบทแปลในตัวบทโฆษณาเครื่องสำอาง

Dam Effects On Freshwater Mussel Growth And Recruitment In A Midwestern Stream, Taylor Lyndon Fagin

Masters Theses

There have been many studies that examine the negative impact of dams on freshwater mussel populations. However, recent studies suggest that some dams may create more suitable growing conditions immediately below some dams. This study’s main objectives were to 1) document where faster growth of mussels is found in relation to the Charleston Dam, 2) determine if sites with faster growth have higher recruitment and 3) investigate which water quality variable(s) could be correlated with faster growth. Live Corbicula sp. were set in cages in multiple sites at varying distances from the dam and shells of two native mussel species …

How Leaders In Greek Life View Alcohol, Blake Miller

Masters Theses

The researcher in this study used qualitative methodology to explore perspectives of undergraduate student leaders in fraternity and sorority life at a mid-sized, Midwest institution on alcohol, especially within the Greek life system. Eight informants participated in semi-structured, open-ended interviews and were asked about their views on and experiences with alcohol, both as a new member and after having leadership experience. Informants were also asked about alcohol intervention and prevention measures. A majority of informants maintained or developed an indifferent or aversive view of alcohol, had decreased their personal levels of alcohol use, had made attempts to change the way …

Transcriptome Analysis Of Neuro-2a Cells Treated With Asiatic And Madecassic Acid, Fatimah M. Alqam

Masters Theses

Traditional herbal medicine is ingrained as a source of therapeutic compounds to medicate various diseases. The family Araliaceae (Ginseng family) is rich in traditional medicine species, such as Centella asiatica (CA). For many centuries, CA has been used by the indigenous Indian and Chinese in Ayurvedic and traditional medicine, respectively, to improve intelligence, learning, memory, and cognitive performance. Previous studies on cell culture and animal models supported the beneficial effects of CA on the nervous system. However, the exact composition of CA extract and its molecular mechanism that leads to neuroprotection is still unclear. We examined the effect of asiatic …

The Cast Of A Giant's Shadow, Angela Kay Steineman

Masters Theses

Adapting fairy tales and folklore has been an ongoing endeavor by storytellers and artists since the very first story was repeated. The evidence can be seen in the many versions of fairy tales like those of the sleeping beauty, from Giambattista Basile’s “Sun, Moon, and Talia” to Walt Disney’s Maleficent. However, unlike their European counterparts, adaptations of American tales outside of children’s literature are not as ubiquitous. My writing rectifies this by adding to the resurging interest as seen in recent retellings like Matt Bell’s Appleseed: The Monstrous Birth (2019).

In an effort to reframe the American tall tale …

The Impact Of Shared Reading On 1st Graders’ Writing Using Author’S Craft, Kelsey Bolin

Masters Theses

This study was conducted in a first grade classroom with twenty participants. This study took place over five and a half weeks, where students were introduced to three mentor texts in a shared reading setting. The students asked and answered questions about the texts and participated in extension writing activities. Students were given opportunities to engage with the mentor texts and writing in whole group settings as well as individual settings. Students were expected to write a final assignment using the form of author craft portrayed in the final mentor text, which students were able to complete successfully.

Students practiced …

Is Context Dependency Imperative To Understanding The Impacts Of Invasive Plants?, Brendan B. Haile

Masters Theses

Introduced exotic species have a tendency to become invasive and impact local biological communities. Invasions often impact community attributes such as cover and species richness, but these factors may also regulate patterns of invasion. In such cases, impacts may be dependent on the invasion context. We used data from the Buell-Small Succession Study, a long-term permanent plot study in the piedmont region of New Jersey, to document context dependency in invasion. To do this, we analyzed the factors that affected the colonization and growth of four invasive species, Alliaria petiolata, Lonicera japonica, Microstegium vimineum and Rosa multiflora, as well …

Mass Spectrometry Based Lipidomic Investigation Of Staphylococcus Aureus Lipid Metabolism And Characterization Of Lipase A, Naren Gajenthra Kumar

Theses and Dissertations

Staphylococcus aureus produces an arsenal of virulence factors and is adept at colonizing multiple hosts by employing specific processes to persist at different sites of infection. Infections caused by this pathogen range from self-limiting infections of the skin like folliculitis to life threatening and chronic conditions like infective endocarditis, non-healing diabetic foot ulcers and atopic dermatitis. As the field continues to expand its understanding of the mechanisms by which this pathogen rapidly develops resistance to new antibiotics, the underlying mechanisms that facilitates niche adaptation and host colonization among healthy individuals remain elusive.

Bacterial and host lipid metabolism presents and area …

Pharmacological Characterization Of Novel Low Efficacy Mor Modulators: Implications For Opioid Use Disorder Treatment, Abdulmajeed Jali

Theses and Dissertations

Opioid use disorder (OUD) is a major public health concern in the Unites States, resulting in an estimated 128 overdose deaths per day. Although presently approved OUD pharmacotherapies are partially effective, they are accompanied by undesirable side effects or low compliance. m-Opioid receptor (MOR) agonists (methadone and buprenorphine) are associated with physical dependence, abuse liability and respiratory depression, whereas the MOR antagonist naltrexone is associated with withdrawal precipitation, dysphoria and low patient compliance. Therefore, development of novel medications with fewer side effects and higher compliance than current OUD pharmacotherapies is needed. The objective of this dissertation was to pharmacologically characterize …

Development Of An Anatomically And Electrically Conductive Brain Phantom For Transcranial Magnetic Stimulation, Hamzah A. Magsood

Theses and Dissertations

Transcranial Magnetic Stimulation (TMS) is a non-invasive technique for diagnostics, prognostic, and treatments of various neurological diseases. However, the lack of anatomically realistic brain phantoms has made the experimental verification of stimulation strength in the form of induced electric fields/voltages in the brain tissues an impediment to developing new TMS coils, stimulators, and treatment protocols. There are significant technological, safety, and ethical limitations to test the potential TMS treatment procedures or develop enhancements and refine them on humans or animals. This work aims to bridge the gap by introducing and developing an innovative manufacturing and fabrications process to produce a …

Assessing The Role Of Planar Cell Polarity Protein Ptk7 In Axon Pathfinding Of Spinal Commissural Neurons, Joseph Paul Miano

Theses and Dissertations

Axon pathfinding is a fundamental step that establishes the vast networks of the nervous system. Understanding this process is important because disruptions to these neural networks can impair quality of life. During axon pathfinding, an axon’s growth cone comes in contact with a variety of secreted chemotropic signaling cues that control growth cone behavior through activation of signal transduction pathways which cause changes to the cytoskeleton. One such pathway is the Planar Cell Polarity (PCP) signal transduction pathway that responds to gradients of non-canonical Wnt ligands. PCP has a well-known role in axon pathfinding, including axon pathfinding in the spinal …

Ndrg1 And Myelin-Related Disease: Alcoholism And Chemotherapy-Induced Neuropathy, Guy Harris

Theses and Dissertations

Alcohol use disorder (AUD) is a prevalent neuropsychiatric disease with profound health, social, and economic consequences. With an estimated 50% heritability, identifying genes that engender risk and contribute to the underlying neurobiological mechanisms represents an important first step in developing effective treatments. Gene expression studies are an important source of candidate genes for studying AUD, providing windows into the molecular machinery engaged by the brain in response to ethanol. Our laboratory has implicated N-myc down-regulated gene 1 (Ndrg1) as a potential candidate gene that modulates ethanol-induced changes in myelin-related gene expression and acute sensitivity to ethanol. Analysis of …

Transcriptomic Profiling Of Postmortem Prefrontal Cortex And Nucleus Accumbens From Chronic Alcohol Abusers., Eric S. Vornholt

Theses and Dissertations

Alcohol use disorder (AUD) is a debilitating psychiatric illness that develops from a combination of genetic and environmental factors. While it is well documented that AUD is heritable, the shift from recreational alcohol use to abuse/dependence is poorly understood. In this dissertation, using postmortem brain tissue from individuals with alcohol dependence (AD), we profiled the genome-wide expression of circular RNA (circRNA), microRNA (miRNA), and messenger RNA (mRNA) to better understand the impact of gene expression on the development of AUD. To achieve this, we performed two independent studies that explore transcriptome differences between AD cases and controls. The first of …

Impact Of A Culturally-Relevant Support Program And Latinx Adolescents' Help Seeking Services: An Academic-Latinx Community Service Partnership To Enhance Culturally Responsive Services For Latinx Immigrant Youth., Lisa S. Fuentes

Theses and Dissertations

Latinxs, in general, report logistical barriers that contribute to utilizing fewer healthcare services and/or not knowing how to navigate the healthcare system. In addition to underutilizing healthcare services, Latinx immigrant youth also report higher levels of negative attitudes towards mental health. However, little is still known regarding factors that impact these attitudes with Latinx immigrant youth, as well as the community support efforts that are available to this specific group. Using a semi-structured interview on 12 Latinx immigrant youth, this master’s thesis explored: (1) the attitudes towards mental health, (2) attitudes towards mental health services, (3) and the experiences of …

The Effects Of An Inflammasome Inhibitor Olt1177 On The Development Of Ischemic Heart Failure, Joseph S. Aliaga

Theses and Dissertations

Thesis Abstract

THE EFFECTS OF AN INFLAMMASOME INHIBITOR OLT1177 ON THE DEVELOPMENT OF ISCHEMIC HEART FAILURE

By Joseph S. Aliaga, BS

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science at Virginia Commonwealth University.

Virginia Commonwealth University, 2020

Background: Evidence suggests that prolonged and enhanced pro-inflammatory signaling, modulated by the NLRP3 inflammasome, plays a crucial role in the pathophysiology of several different types of cardiovascular diseases such as acute myocardial infarction (AMI), adverse ventricular remodeling, and heart failure (HF). Consequently, we hypothesize that attenuating the enhanced inflammatory response using a pharmacological NLRP3 …

Novel Strategies To Overcome Carboplatin Resistance In Triple Negative Breast Cancer Using Patient Derived Xenografts, Mohammad A. Al Zubi

Theses and Dissertations

Triple-negative breast cancer (TNBC) patients have a poor prognosis and rely on chemotherapeutic treatment as standard of care. Often, they develop chemotherapy resistance, which leaves them without more therapeutic options, like targeted therapy. New models have been developed to test targeted inhibitors in human tumors, and they are known as patient-derived xenografts (PDX). These tumors are obtained from patients, then established and maintained in mice where they areused for tumor studies. In this work, we characterized 14 PDXs for their primary tumor growth rate and investigated metastatic propensity using spontaneous and experiment metastasis models. We utilized RNA-sequencing to characterize contributions …

Analysis Of Stigmatic Content In State Mental Health Legislative Proposals, David L. Conley

Theses and Dissertations

Despite the proven effectiveness of mental health interventions, services remain limited across the country. Social workers have repeatedly advocated for increased funding, but mental healthcare gaps persist. Disparities could be addressed through the policy process, but critical proposals often do not pass. One of the biggest barriers is the concept of stigma, which could extend into legislatures and influence mental health-related policy outcomes as a form of structural stigma. Factors that influence legislator voting behavior are found in the literature, but studies have not explicitly focused on structural stigma or mental health-specific policy outcomes. Thus, the present study aimed to …

The Role Of Interleukin-18 In Myocardial Ischemia/Reperfusion, Yogesh Dhakal

Theses and Dissertations

Acute Myocardial Infarction (AMI) is one of the leading causes of morbidity for cardiovascular diseases (CVDs) in the western world. While mortality rates for AMI has reduced in the last 20 years due to advancements in clinical interventions, patients who have an AMI are significantly more likely to develop heart failure (HF) in the following years. Reperfusion of the ischemic tissue is a necessary procedure to restore blood delivery to cardiomyocytes, minimize myocardial dysfunction, and reduce infarct size. Still, the step of reperfusion itself induces myocardial damage in a phenomenon known as reperfusion injury. Considering that the degree of myocardial …

Focal Augmentation Of Somatostatin Interneuron Function And Subsequent Circuit Effects In Developmentally Malformed, Epileptogenic Cortex, Nicole Ekanem

Theses and Dissertations

Drug-resistant epilepsy (DRE) is a common clinical sequela of developmental cortical malformations such as polymicrogyria. Unfortunately, much remains unknown about the aberrant GABA-mediated circuit alterations that underlie DRE's onset and persistence in this context. To address this knowledge gap, we utilized the transcranial freeze lesion model in optogenetic mice lines (Somatostatin (SST)-Cre or Parvalbumin (PV)-Cre x floxed channelrhodopsin-2) to dissect features of the SST, PV, and pyramidal neuron microcircuit that are potentially associated with DRE. Investigations took place within developmental microgyria’s known pathological substrate, the adjoined and epileptogenic paramicrogyral region (PMR). As well, microcircuit relationships within the previously unexplored range …

Spag17 Is Important For Protein Trafficking In Mammalian Spermiogenesis, Virali Bhagat

Theses and Dissertations

Spermiogenesis is the process through which undifferentiated germ cells develop into mature spermatozoa. While spermiogenesis is a very well-regulated process, the protein-protein interactions regulating it remain poorly understood. A knockout (KO) mouse for the ciliary protein SPAG17 was generated by our lab. Loss of SPAG17 has been shown to disrupt the transport of proteins important for acrosome biogenesis and manchette functions. With this information, we hypothesized that SPAG17 plays an essential role in protein trafficking during mammalian spermiogenesis. To further investigate this, immunofluorescence (IF) studies were performed in germ cells collected from both WT and SPAG17 KO mice to visualize …