Actuopaleoichnology Of A Modern Bay Of Fundy Macro-Tidal Flat: Analogy With A Mississippian Tidal Flat Deposit (Hartselle Sandstone) From Alabama, Louis G. Zachos, Brian F. Platt

Faculty and Student Publications

Copyright 2019 Zachos and Platt Trace fossil zonation in the Hartselle Sandstone of Mississippian age (Chesterian: Visean-Serpukhovian) exposed on Fielder Ridge, Alabama is compared with modern macro-tidal flat ichnocoenoses on the Bay of Fundy at Lubec, Maine, and demonstrated to be analogous by sedimentologic and ichnotaxonomic criteria. The modern flat has minimal influence from either waves or freshwater influx, and can be divided into five distinct ichnocoenoses, characterized by surface traces (epichnia) and four sedimentologic facies defined by gross grain texture or hydrodynamic characteristics, but lacking significant surface traces. Several characteristics of tidal flat deposits in a fetch-limited, marine (i.e., …

Virginia Commonwealth University Fall Commencement Program [2019], Virginia Commonwealth University

VCU Commencement Programs

No abstract provided.

Vcu Brew, The Grace E. Harris Leadership Institute At Virginia Commonwealth University, Denise Bullock, Lauren Dunn, Brian Fowlkes, Hermine Maes, Elizabeth Marlowe, Fernando Tenjo-Fernandez, Zakia Williams

The Grace E. Harris Leadership Institute Publications

VCU Brew is an innovative proposal to create a VCU branded signature beer. The Campus Connectors will present a rationale showing how this proposal will i) potentially generate revenue to benefit VCU scholarships and academic programs, ii) create opportunities for experiential learning by engaging VCU students in product development, branding, labeling, marketing, business, and awareness of responsible drinking, iii) and guide the process of partnering with local breweries to provide practical expertise and stimulate entrepreneurship initiatives that lead to distribution at VCU athletic, community and Alumni events, while increasing ties with the community and national prominence.

Grief And Color In A. E. Coppard’S “The Princess Of Kingdom Gone”, Sydney Sterrett

Modernist Short Story Project

Only about a year after the horrors of World War I, England was doing its best to reestablish itself as a seat of cultural and artistic value. Many journals and magazines ran new poetry and stories that were meant to relive war time or move on from it, but nearly everything seemed to be colored by the sights that the surviving young men had seen in the trenches. In November of 1919, A. E. Coppard published a short story in the Voices of Poetry and Prose magazine—a magazine that was meant to help readers recover from the war through new, …

Hg Wells’ Anticipations : More “Perishable” Feminism, Kacey Sorenson

Modernist Short Story Project

In researching H.G. Wells’ evolving views on eugenics, race, anti-Semitism, and women, there was a noticeable absence of scholars referring to his last chapter of Anticipations of the Reaction of Mechanical and Scientific Progress upon Human Life and Thought. Regardless of why it has been overlooked, the aim of this study is to use the last chapter of Anticipations specifically to emphasize and confirm what feminist scholars have extracted as Wells’ view of women: what he proudly owned as feminism was dismissed by his contemporaries as “very perishable” (Kirchwey 308).

Analyzing The Hypervolume Of Plant Functional Traits In The Sierra Nevada Mountain Range, Rosina Garcia, David Schimel, Ryan Pavlick

STAR Program Research Presentations

Studying Earth’s biodiversity is important in understanding how ecosystems are changing in response to environmental stressors. Particularly, monitoring Earth via remote sensing can give us high-resolution data of plant functional traits, such as canopy height, leaf mass per area (LMA), and leaf nitrogen content, which provide insight into ecosystem changes. We mapped out remote sensing airborne data from the Sierra Nevada Mountain Range in California and used R to create a hypervolume of the vegetated area. Hypervolumes are useful in creating a three-dimensional visualization of data to understand the relationships among different traits. We computed the three-dimensional space of the …

Survival And Growth In Experimental Plots At A Santa Rosa Island Restoration Site, 2016-2018, Marisol Gonzalez Villarreal, Robyn Shea, Aspen Coty

STAR Program Research Presentations

Survival and Growth in Experimental Plots at a Santa Rosa Island Restoration Site, 2016-2018 Marisol Villarreal1, Stephen Bednar2, Kathryn McEachern3 1Woodland Joint Unified School District, 2Mountains Restoration Trust, 3United States Geological Survey Restoration biology, the practice of healing a damaged landscape to that of a functioning ecosysytem, is as variable a science as they come. Restoration treatments may take into account precipitation, symbiotic relationships, microclimate, degree of plant succession, and soil conditions. The most effective restoration is based on experimental research. Such is the goal for the Cloud Forest Restoration Project on Santa Rosa Island, within Channel Islands National Park …

Method Development For Structural Assessment Of Nanolipoprotein Particles With And Without Cross-Linked Lipids, Emma J. Mullen, Wei He, Sean Gilmore, Matthias Frank, Matthew Coleman, Megan Shelby

STAR Program Research Presentations

Membrane proteins make up approximately 30% of the cellular proteome and account for over 60% of pharmaceutical targets.1 Determining the structures of this class of proteins is critical to our understanding of disease states and will advance rational drug design. But membrane proteins have limited solubility, rarely form large crystals that diffract well, and often misfold outside of a bilayer, hindering crystallographic studies.1 Nanolipoprotein particles (NLPs) have arisen as a platform to readily solubilize membrane proteins while mimicking a native lipid environment. NLPs consist of a discoidal phospholipid bilayer encircled by an apolipoprotein belt. In an effort to optimize and …

Comparing Regional Population Genetic Effects In Leptasterias Spp., Kristen N. Runge, Sarah Cohen

STAR Program Research Presentations

Leptasterias spp. is a species complex of six-rayed intertidal sea stars found along the northeast Pacific coast. A developmental mode of brooding their young, rather than broadcasting for planktonic development, limits dispersal away from the natal area, and may also result in local adaptation of populations. Local adaptation may lead to morphological and behavioral differences among populations. Previous studies on behavior in Leptasterias spp. have shown that activity levels may vary by population and region. Across three regions from California to Washington, differences were only observed between central California and more northern regions while northern California, Oregon, and Washington were …

The Unacceptable Rise: An Investigation Of School Shootings In The United States, Tracy Gulliford

Electronic Theses and Dissertations

This research study examined teachers' understanding of risk factors and characteristics of potential perpetrators of school shootings, as well as the steps to take when they are identified in students. Participants completed a survey that contained Likert-type scale items regarding self efficacy and open-ended questions regarding school violence experiences. A mixed methods analysis revealed three primary conclusions. First, teachers have high levels of self-efficacy regarding the importance of and ability to deal effectively with troubled students. This conclusion suggests that teachers do internalize the relevance of this issue to them. Second, this study revealed a gap in terms of the …

The Feminist Question: A Close Look At Feminism In Dan Brown’S The Da Vinci Code, Marley Howell

First Class: A Journal of First-Year Composition

No abstract provided.

2019 Symposium Brochure

Programs

No abstract provided.

Demineralized Bone Matrix Combined Bone Marrow Mesenchymal Stem Cells, Bone Morphogenetic Protein-2 And Transforming Growth Factor-Β3 Gene Promoted Pig Cartilage Defect Repair, Xin Wang, Yanlin Li, Rui Han, Chuan He, Guoliang Wang, Jianwei Wang, Jiali Zheng, Mei Pei, Lei Wei

Faculty & Staff Scholarship

Objectives

To investigate whether a combination of demineralized bone matrix (DBM) and bone marrow mesenchymal stem cells (BMSCs) infected with adenovirus-mediated- bone morphogenetic protein (Ad-BMP-2) and transforming growth factor-β3 (Ad-TGF-β3) promotes the repair of the full-thickness cartilage lesions in pig model.

Methods

BMSCs isolated from pig were cultured and infected with Ad-BMP-2(B group), Ad-TGF-β3 (T group), Ad-BMP-2 + Ad-TGF-β3(BT group), cells infected with empty Ad served as a negative group(N group), the expression of the BMP-2 and TGF-β3 were confirmed by immunofluorescence, PCR, and ELISA, the expression of SOX-9, type II collagen(COL-2A), aggrecan (ACAN) in each group were evaluated by …

Differences Between Occupational And Non-Occupational-Related Motor Vehicle Collisions In West Virginia: A Cross-Sectional And Spatial Analysis, Toni Marie Rudisill

Faculty & Staff Scholarship

Background

Motor vehicle collisions comprise the majority of occupational-related fatalities in the United States and West Virginia has one of the highest occupational-related fatality rates in the nation. The purpose of this study was to compare work and non-work-related collisions, crash locations, and the characteristics of in-state and out-of-state drivers ≥18 years of age who were fatally injured in work-related collisions in West Virginia.

Methodology

Data were from the 2000–2017 Fatality Analysis Reporting System. Work and non-work-related crashes and characteristics in-state vs. out-of-state drivers were compared using binary and multivariable logistic regression analyses. Crash locations were compared via spatial analyses …

Neutrophil Extracellular Traps In Breast Cancer And Beyond: Current Perspectives On Net Stimuli, Thrombosis And Metastasis, And Clinical Utility For Diagnosis And Treatment, Hunter T. Snoderly, Brian A. Boone, Margaret F. Bennewitz

Faculty & Staff Scholarship

Abstract

The formation of neutrophil extracellular traps (NETs), known as NETosis, was first observed as a novel immune response to bacterial infection, but has since been found to occur abnormally in a variety of other inflammatory disease states including cancer. Breast cancer is the most commonly diagnosed malignancy in women. In breast cancer, NETosis has been linked to increased disease progression, metastasis, and complications such as venous thromboembolism. NET-targeted therapies have shown success in preclinical cancer models and may prove valuable clinical targets in slowing or halting tumor progression in breast cancer patients. We will briefly outline the mechanisms by …

Melatonin Prevents Osteoarthritis-Induced Cartilage Degradation Via Targeting Microrna-140, Yijian Zhang, Jun Lin, Xinfeng Zhou, Xi Chen, Angela Carley Chen, Bin Pi, Guoquing Pan, Ming Pei, Huilin Yang, Tao Liu, Fan He

Faculty & Staff Scholarship

Abstract

Osteoarthritis (OA) is characterized by the progressive destruction of articular cartilage, which is involved in the imbalance between extracellular matrix (ECM) synthesis and degradation. MicroRNA-140-5p (miR-140) is specifically expressed in cartilage and plays an important role in OA-induced matrix degradation. The aim of this study was to investigate (1) whether intra-articular injection of melatonin could ameliorate surgically induced OA in mice and (2) whether melatonin could regulate matrix-degrading enzymes at the posttranscriptional level by targeting miR-140. In an in vitro OA environment induced by interleukin-1 beta (IL-1β), melatonin treatment improved cell proliferation of human chondrocytes, promoted the …

Fourteen Polymorphic Microsatellite Markers For The Widespread Labrador Tea (Rhododendron Groenlandicum), Mathew L. Sheik, Kitty L. Labounty, Erika Mitchell, Emily L. Gillespie

Faculty & Staff Scholarship

PREMISE: Microsatellite markers were developed for Labrador tea (Rhododendron groenlandicum, Ericaceae) to facilitate downstream genetic investigation of this species and the extremely closely related, circumboreal Rhododendron subsect. Ledum. METHODS AND RESULTS: Forty-eight primer pairs were designed using Illumina data and screened for excellent amplification. Sixteen successful pairs were developed as microsatellite markers using fluorescently labeled amplification to generate chromatogram data. These data were evaluated for intrapopulation and interpopulation variability in three populations from Alaska and Maine, USA, and the Northwest Territories, Canada. Fourteen polymorphic markers genotyped reliably, each with one to eight alleles. Cluster analysis indicates that across the range, …

Emerging Insights Of Tumor Heterogeneity And Drug Resistance Mechanisms In Lung Cancer Targeted Therapy, Zuan-Fu Lim, Patrick C. Ma

Faculty & Staff Scholarship

Abstract

The biggest hurdle to targeted cancer therapy is the inevitable emergence of drug resistance. Tumor cells employ different mechanisms to resist the targeting agent. Most commonly in EGFR-mutant non-small cell lung cancer, secondary resistance mutations on the target kinase domain emerge to diminish the binding affinity of first- and second-generation inhibitors. Other alternative resistance mechanisms include activating complementary bypass pathways and phenotypic transformation. Sequential monotherapies promise to temporarily address the problem of acquired drug resistance, but evidently are limited by the tumor cells’ ability to adapt and evolve new resistance mechanisms to persist in the drug environment. Recent …

Dna Methylation And Mirna-1296 Act In Concert To Mediate Spatiotemporal Expression Of Kpna7 During Bovine Oocyte And Early Embryonic Development, Lei Wang, Jacqelyn M. Hand, Liyuan Fu, George W. Smith, Jianbo Yao

Faculty & Staff Scholarship

Abstract

Background: Epigenetic regulation of oocyte-specific maternal factors is essential for oocyte and early embryonic development. KPNA7 is an oocyte-specific maternal factor, which controls transportation of nuclear proteins important for early embryonic development. To elucidate the epigenetic mechanisms involved in the controlled expression of KPNA7, both DNA methylation associated transcriptional silencing and microRNA (miRNA)-mediated mRNA degradation of KPNA7 were examined.

Results: Comparison of DNA methylation profiles at the proximal promoter of KPNA7 gene between oocyte and 6 different somatic tissues identified 3 oocyte-specific differentially methylated CpG sites. Expression of KPNA7 mRNA was reintroduced in bovine kidney-derived CCL2 cells after treatment …

Drilling Performance Monitoring And Optimization: A Data-Driven Approach, Shan E Zehra Lashari, Ali Takbiri-Borujeni, Ebrahim Fathi, Ting Sun, Reza Rahmani, Mehdi Khazaeli

Faculty & Staff Scholarship

Abstract Drilling performance monitoring and optimization are crucial in increasing the overall NPV of an oil and gas project. Even after rigorous planning, drilling phase of any project can be hindered by unanticipated problems, such as bit balling. The objective of this paper is to implement artifcial intelligence technique to develop a smart model for more accurate and robust real-time drilling performance monitoring and optimization. For this purpose, the back propagation, feed forward neural network model was developed to predict rate of penetration (ROP) using diferent input parameters such as weight on bit, rotations per minute, mud fow (GPM) and …

A Review Of 2d And 3d Plasmonic Nanostructure Array Patterns: Fabrication, Light Management And Sensing Applications, Sujan Kassani, Katherine Curtin, Nianqiang Wu

Faculty & Staff Scholarship

Abstract: This review article discusses progress in surface plasmon resonance (SPR) of two-dimensional (2D) and three-dimensional (3D) chip-based nanostructure array patterns. Recent advancements in fabrication techniques for nano-arrays have endowed researchers with tools to explore a material’s plasmonic optical properties. In this review, fabrication techniques including electron-beam lithography, focused-ion lithography, dip-pen lithography, laser interference lithography, nanosphere lithography, nanoimprint lithography, and anodic aluminum oxide (AAO) template-based lithography are introduced and discussed. Nano-arrays have gained increased attention because of their optical property dependency (lightmatter interactions) on size, shape, and periodicity. In particular, nano-array architectures can be tailored to produce and tune plasmonic …

Impact Of Fibronectin Knockout On Proliferation And Differentiation Of Human Infrapatellar Fat Pad-Derived Stem Cells, Yiming Wang, Yawen Fu, Zuoqin Yan, Xiao-Bing Zhang, Ming Pei

Faculty & Staff Scholarship

Fibronectin plays an essential role in tissue development and regeneration. However, the effects of fibronectin knockout (FN1-KO) on stem cells’ proliferation and differentiation remain unknown. In this study, CRISPR/Cas9 generated FN1-KO in human infrapatellar fat pad-derived stem cells (IPFSCs) was evaluated for proliferation ability including cell cycle and surface markers as well as stemness gene expression and for differentiation capacity including chondrogenic and adipogenic differentiation. High passage IPFSCs were also evaluated for proliferation and differentiation capacity after expansion on decellularized ECM (dECM) deposited by FN1-KO cells. Successful FN1-KO in IPFSCs was confirmed by Sanger sequencing and Inference of CRISPR Edits …

Patellar Tendon Morphology In Trans-Tibial Amputees Utilizing A Prosthesis With A Patellar-Tendon-Bearing Feature, Kai-Yu Ho, Michelle Harty, Jessica Kellogg, Kelly Teter, Szu-Ping Lee, Yu-Jen Chang, Gregory Bashford

Faculty & Staff Scholarship

A patellar-tendon-bearing (PTB) bar is a common design feature used in the socket of trans-tibial prostheses to place load on the pressure-tolerant tissue. As the patellar tendon in the residual limb is subjected to the perpendicular compressive force not commonly experienced in normal tendons, it is possible for tendon degeneration to occur over time. The purpose of this study was to compare patellar tendon morphology and neovascularity between the residual and intact limbs in trans-tibial amputees and healthy controls. Fifteen unilateral trans-tibial amputees who utilized a prosthesis with a PTB feature and 15 age- and sex- matched controls participated. Sonography …

Surgical Correction Of Aortic Regurgitation Using A Haart 300™ Rigid Aortic Ring: A Novel Method To Standardize Aortic Valve Repair, Radoslaw Gocol, Marek Jasiński, Damian Hudziak, Jaroslaw Bis, Aleksandra Żak, Piotr Duraj, Magdalena Mizia, J. Scott Rankin, Marek A. Deja

Faculty & Staff Scholarship

No abstract provided.

Comparison Of Long-Term Survival Of Patients With Early-Stage Non–Small Cell Lung Cancer After Surgery Vs Stereotactic Body Radiotherapy, Alexander Chi, Wei Fang, Yeping Sun, Sinjin Wen

Faculty & Staff Scholarship

IMPORTANCE Previous comparisons of surgery and stereotactic body radiotherapy (SBRT) for early-stage (ES) non–small cell lung cancer (NSCLC) did not account for the extent of regional lymph node examination (LNE) during surgery. OBJECTIVE To compare long-term overall survival (OS) of patients with ES NSCLC after surgery vs SBRT when the extent of regional LNE in patients undergoing surgery is thoroughly considered. DESIGN, SETTING, AND PARTICIPANTS Cohort study with survival comparisons using the multivariable Cox proportional hazards model and after propensity score matching. Data from the National Cancer Database were analyzed from October 28, 2018, through April 18, 2019. Patients with …

The Influence Of Location On Water Quality Perceptions Across A Geographic And Socioeconomic Gradient In Appalachia, Ross G. Andrew, Robert C. Burns, Mary E. Allen

Faculty & Staff Scholarship

Abstract: Understanding perceptions of water quality held by residents is critical to address gaps in public awareness and knowledge and may provide insight into what defines communities that are more/less resilient to changing water conditions locally. We sought to identify spatial patterns of water quality perceptions gathered in a survey of Southern West Virginia (WV) residents during spring/summer 2018. Using over 500 survey responses across 15 counties, we calculated spatial autocorrelation metrics and modeled the relationships between overall water quality perceptions and county-level socioeconomic endpoints, such as poverty rate, per capita income, and education level. We identified significant differences across …

Cannabis And Psychosis Through The Lens Of Dsm-5, Nathan T. Pearson, James H. Berry

Faculty & Staff Scholarship

Evidence for an association between cannabis and psychosis has been documented in literature in many forms including experimental studies, epidemiological data, and case series. The association has implications for psychotic outcomes ranging from mild to severe and occurring over minutes to years. Due to the huge variety of exposures and outcome measures reported, creating a coherent account of all the available information is difficult. A useful way to conceptualize these wide-ranging results is to consider the association between cannabis and psychosis as it occurs within the context of widely used DSM-5 diagnoses. In the present review we examine cannabis/psychosis associations …

A Case-Study Application Of The Experimental Watershed Study Design To Advance Adaptive Management Of Contemporary Watersheds, Jason A. Hubbart, Elliott Kellner, Sean J. Zeiger

Faculty & Staff Scholarship

settings Open AccessFeature PaperArticle

A Case-Study Application of the Experimental Watershed Study Design to Advance Adaptive Management of Contemporary Watersheds

by Jason A. Hubbart 1,*,Elliott Kellner 2 andSean J. Zeiger 3 1 West Virginia University, Institute of Water Security and Science, Davis College of Agriculture, Natural Resources and Design, Schools of Agriculture and Food, and Natural Resources, 3109 Agricultural Sciences Building, Morgantown, WV 26506, USA 2 West Virginia University, Institute of Water Security and Science, Davis College of Agriculture Natural Resources and Design, Division of Plant and Soil Sciences, 3011 Agricultural Sciences Building, Morgantown, WV 26506, USA 3 …

Comparison Of Whole-Genome Sequences Of Legionella Pneumophila In Tap Water And In Clinical Strains, Flint, Michigan, Usa, 2016, Emily Garner, Connor L. Brown, David Otto Schwake, William J. Rhoads, Gustavo Aragon-Argoty, Liqing Zhang, Guillaume Jospin, David A. Coil, Jonathan A. Eisen, Marc A. Edwards, Amy Pruden

Faculty & Staff Scholarship

During the water crisis in Flint, Michigan, USA (2014–2015), 2 outbreaks of Legionnaires’ disease occurred in Genesee County, Michigan. We compared whole-genome sequences of 10 clinical Legionella pneumophila isolates submitted to a laboratory in Genesee County during the second outbreak with 103 water isolates collected the following year. We documented a genetically diverse range of L. pneumophila strains across clinical and water isolates. Isolates belonging to 1 clade (3 clinical isolates, 3 water isolates from a Flint hospital, 1 water isolate from a Flint residence, and the reference Paris strain) had a high degree of similarity (2–1,062 single-nucleotide polymorphisms), all …

Increased Dementia Mortality In West Virginia Counties With Mountaintop Removal Mining?, A. K. Salm, Michael J. Benson

Faculty & Staff Scholarship

(MTM), a practice that has been ongoing in some counties of West Virginia (WV) USA since the 1970s. PM inhalation has been linked to central nervous system pathophysiology, including cognitive decline and dementia. Here we compared county dementia mortality statistics in MTM vs. non-MTM WV counties over a period spanning 2001–2015. We found significantly elevated age-adjusted vascular or unspecified dementia mortality/100,000 population in WV MTM counties where, after adjusting for socioeconomic variables, dementia mortality was 15.60 (±3.14 Standard Error of the Mean (S.E.M.)) times higher than that of non-MTM counties. Further analyses with satellite imaging data revealed a highly significant …