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Environmental Sampling Techniques For Herd-Level Surveillance Of Bovine Viral Diarrhea Virus, Jaden Carlson

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Control of Bovine Viral Diarrhea Virus (BVDV) relies on resource-intensive individual animal sampling to detect and remove persistently infected (PI) cattle. Herd-level surveillance tools would be useful for herds with unknown BVDV status and for monitoring herds with BVDV-free status. The overall objective of this thesis is to explore the viability of BVDV surveillance at a herd-level using samples collected without handling individual animals. The first objective was to determine the feasibility of using stable flies as a sampling tool to detect BVDV. The second objective was to determine the feasibility of using drinking water to detect BVDV. To accomplish …

Conocimiento, Actitudes Y Prácticas Sobre El Zika: Implicancias Programaticas Para La Prevencion Del Zika En Honduras, Breakthrough Research

Poverty, Gender, and Youth

This brief provides key insights for implementers of social and behavior change activities for Zika prevention in Honduras, based on data from a representative sample of people living in selected USAID Zika program implementation communities, supporting an evidence-based approach to increase Zika and other Aedes aegypti-transmitted virus prevention behaviors.

Climate Change And Chagas Disease In The Americas: A Qualitative Systematic Review, Brittany N. Graves

Dissertations and Theses (Open Access)

While many studies have found associations between climate change and factors affecting Chagas disease transmission, the future impact of climate change on the global spread of Chagas disease remains debatable. A qualitative, systematic review was conducted to assess the impact of climate change on Chagas disease transmission in the Americas (Central America, South America, and North America). The literature search was performed in January 2019 using the keywords climate, Chagas, and “trypanosoma cruzi” and the electronic databases PubMed, Scopus, and Ovid. Searches retrieved records from 1982 through 2019. The initial electronic database search identified a total of 191 record documents …

Hepatitis C Infection In Men Who Have Sex With Men, Jing Zhao

Dissertations and Theses (Open Access)

Hepatitis C virus (HCV) infection is the leading cause of cirrhosis, hepatocellular carcinoma, and liver transplantation. Current treatment rates and efficacy are inadequate to manage the disease burden caused by HCV; thus, efforts are needed to control HCV transmission. Besides the well-known transmission routes, which are health-care-associated transmission and transmission through injecting drugs, HCV could also be transmitted by sexual contact, especially among men having sex with men (MSM). MSM also have higher drug use prevalence than the general population, which may contribute to HCV infection by sexual disinhibition and other risky behavior. Since 2000, there have been emerging reports …

Tuberculosis Among Dairy Workers In Bailey County, Texas, Anabel Rodriguez

Dissertations and Theses (Open Access)

On January 2016, Texas State Department of Health Services (DSHS) Public Health Region 1 (PHR 1) conducted T-SPOT.TB tests in response to two requests to screen dairy workers potentially exposed to cattle infected with Mycobacterium bovis or bovine tuberculosis (bTB) in Bailey County, Texas. Out of 140 workers tested, 14 had confirmed latent tuberculosis infection (LTBI)—prevalence of 10.0%. This first study gave rise to questions concerning tuberculosis (TB) knowledge and exposure history among dairy workers in this same county. The second study focused on determining TB knowledge among dairy workers through a series of questions administered by research personnel on …

Optimum Planting Dates For Garlic In Southwest Missouri, Esther A. Nelson

Graduate Theses/Dissertations

Garlic (Allium sativum) is one of the most widely used spices in the world. More research is needed to outline the best growing practices for garlic in Southwest Missouri. The study is designed to look the yield produced by four different fall planting and one spring date. Three varieties of garlic including Inchilium Red, German White and Elephant were planted on each date at two separate locations in Southwest Missouri for one year. The garlic was found to have highly diverse yields at both locations and when planting dates where compared. Elephant garlic produced the highest yield when …

Data-Driven Decision-Support For Process Improvement Through Predictions Of Bed Occupancy Rates, Kar Way Tan, Qi You Ng, Francis Ngoc Hoang Long Nguyen, Sean Shao Wei Lam

Research Collection School Of Computing and Information Systems

Managing bed utilization and ensuring the supply keeps up with the demand is not an easy task in a large public hospital with many medical disciplines. The bed managers who makes decisions on reserving and allocating beds centrally require high-dimensional data from several hospital information systems supporting emergency room, specialized clinics and bed management processes. In this work, we put together an automated process for cleaning, consolidating and integrating data from several hospital information systems to several reports required by the bed managers to analyse the bed occupancy situations across more than thirty medical disciplines. To prevent bed crunch situations …

Factors Associated With Eosinophilic Esophagitis In Nevada, Julia Lorraine Anderson

UNLV Theses, Dissertations, Professional Papers, and Capstones

Eosinophilic esophagitis (EoE) is a rare immune-mediated illness with symptoms that range from difficulty swallowing to food impaction of the esophagus. Most published studies have been documented among patients residing in cool regions with significant annual rainfall. No published studies to our knowledge have been performed examining the healthcare utilization trends of EoE in Nevada. Utilizing two unique databases, the factors associated with EoE healthcare utilization patterns in Nevada were examined. All analyses were performed in R version 3.5.1. This study included a demographic and regional analysis identifying risk factors associated with having an EoE healthcare visit in Nevada. Several …

Preparation, Functionalization, And/Or Characterization By X-Ray Photoelectron Spectroscopy Of Carbon Surfaces For Biosensors And Other Materials, Varun Jain

Theses and Dissertations

My dissertation is primarily divided into two parts. The first deals with the preparation, functionalization, and characterization of carbon surfaces prepared by direct current magnetron sputtering (DCMS) and high power impulse magnetron sputtering (HiPIMS) as substrates for bioarrays. Part two discusses applications of XPS peak fitting in surface chemical analysis. Chapter 1, the introduction, includes (i) a discussion of the construction of bioarrays and the preparation of sputtered surfaces, e.g., by DCMS and HiPIMS, and also functionalization (bioconjugate) chemistry with special emphasis on the importance of covalent functionalization of surfaces, and (ii) a discussion of the surface characterization techniques and …

Effect Of Mixing On Monochloramine Formation Through In-Line Injection Of Chemicals In Water Disinfection Process, Farhikhteh Samadi

Civil Engineering Dissertations - Archive

Addition of chlorine and ammonia to water for monochloramine formation in the secondary disinfection process in treatment plants has some challenges. Monochloramine formation as the only desirable product of the chloramination process is affected by several factors. These factors are pH, chlorine to nitrogen ratio and chemical mixing. In this study an investigation was conducted to measure the effect of mixing on monochloramine formation through direct injection of chemicals in a pipeline when keeping the other factors constant. In this study two sets of experiments were conducted. In the first study a tracer salt testing was used to find a …

Zika-Related Knowledge, Attitudes, And Practices: Programmatic Implications For Zika Prevention In Honduras, Breakthrough Research

Poverty, Gender, and Youth

This brief provides key insights for implementers of social and behavior change activities for Zika prevention in Honduras, based on data from a representative sample of people living in selected USAID Zika program implementation communities, supporting an evidence-based approach to increase Zika and other Aedes aegypti-transmitted virus prevention behaviors.

Conocimiento, Actitudes Y Prácticas Sobre El Zika: Implicancias Programaticas Para La Prevencion Del Zika En Guatemala, Breakthrough Research

Poverty, Gender, and Youth

This brief provides key insights for implementers of social and behavior change activities for Zika prevention in Guatemala. The insights are based on data from a representative sample of people living in select USAID Zika program implementation communities and support an evidence-based approach for programming to increase preventative behaviors for Zika and other Aedes aegypti-transmitted viruses. The data indicate a need to narrow existing knowledge gaps related to the Zika virus, particularly its health consequences and effective preventive practices, and communicate more specifically about preventative behaviors to enable effective action.

Fungal Cervical Abscess Complicated By Necrotizing Fasciitis Leading To Descending Necrotizing Mediastinitis: A Case Report., Zainab Abbasi, Hina Inam, Sajan Das, Saulat Fatimi, Sejal Neel

Section of Cardiothoracic Surgery

Cervical necrotizing fasciitis (CNF) is a rapidly spreading deep neck infection with a high mortality rate if left untreated. The occurrence of necrotizing infections in the head and neck region is uncommon; therefore, it is a rare cause of chest pain presenting to the emergency department. Here, we present an interesting case of fungal cervical skin abscess complicated by necrotizing fasciitis that progressed to involve the mediastinum, causing necrotizing mediastinitis with pneumomediastinum in an elderly female. The patient presented to the emergency department with chest pain, shortness of breath, and fever. She had a 10-day history of a mass in …

Development Of A Rapid Diagnostic Assay For The Colorimetric Detection Of Legionella Bacteria Using Loop Mediated Isothermal Amplification, Tess Peacock

All NMU Master's Theses

Legionella are aquatic bacteria capable of inhabiting man-made water systems. Inhaling water droplets carrying Legionella can cause Legionnaires’ disease, a rapidly progressing pneumonia. From 2000-2016 the number of Legionnaires’ cases increased 5-fold. Detecting Legionella in water systems can be challenging. Legionella have complex growth requirements, requiring expensive specialized media to grow. It can take 10 days to grow Legionella colonies on agar plates. There are few rapid assays available that can identify multiple Legionella species. This project aims to develop a Loop Mediated Isothermal Amplification (LAMP) assay that can detect multiple species of Legionella in less than one hour. LAMP …

How Extrinsic Mortality Affects Age At Menarche And Fertility In A 1970 British Cohort, Kimberly Marie Neagle

Boise State University Theses and Dissertations

Extrinsic mortality is the likelihood of mortality that is not conditional on reproductive effort. It does not depend on a person’s behavior and cannot be changed by altering behavior. Theoretically, extrinsic mortality plays a major role in the evolution of life history and the variation in reproductive strategies. Using life history theory as a framework, with higher extrinsic mortality cues women should speed up reproduction to maximize fitness in uncertain or risky environments, and in environments with little risk, women can allocate their energy to somatic development and in this time, accrue resources such as education and career opportunities. Thus, …

Microbial Colonization Of Pneumatic Tourniquets In The Orthopedic Operating Room, Syed Hamza Mufarrih, Nada Qaisar Qureshi, Rizwan Haroon Rashid, Bilal Ahmed, Seema Irfan, Akbar Jaleel Zubair, Shahryar Noordin

Section of Orthopaedic Surgery

Background: The rate of surgical site infections following orthopedic procedures is approximately 2% globally. Potential sources of contamination in the operating room include pneumatic tourniquets, blood pressure cuffs, and stethoscopes, among others. Our study aims to investigate microbial colonization on reusable pneumatic tourniquets stored and used in the orthopedic department of our institution and evaluate the efficacy of the cleaning protocols employed. Methods: Over a course of two weeks, 26 samples were obtained. A total of 14 pneumatic tourniquets were sampled preoperatively on Monday morning following the weekly cleaning protocol of soaking the tourniquets in sodium hypochlorite for 30 minutes …

Investigation Of Narrow Spectrum Targets In Antibacterial Drug Discovery, Jesse Jones

Theses and Dissertations (ETD)

Background: Significant concerns are associated with the use of broad-spectrum antibacterial agents, including collateral eradication of beneficial bacteria from the human microbiome, the onset of antibacterial-associated infections, and continued emergence of antibacterial drug resistance. As such, a critical need for novel and selective antibacterial targets exists. The investigation of two such targets, each pertaining to the highly concerning infections caused by streptococcal species and Clostridioides difficile, are presented herein. Bacterial topoisomerase I represents a potentially promising narrow-spectrum target as studies have arisen demonstrating its essentiality in bacterial species lacking the only other type IA topoisomerase (topoisomerase III). Additionally, recent studies …

Estimated Costs Of Injuries In College And High School Female Sports, Ray C. Fair, Christopher Champa

Cowles Foundation Discussion Papers

Injury rates in thirteen U.S. women’s college sports and four U.S. girls’ high school sports are examined in this paper. The sports are categorized as high injury (H) or low injury (L) and differences in injury rates between the two are examined. Estimates are presented of the injury savings that would result if the H sports were changed to have injury rates similar to those in the L sports. The estimated college savings are 13,610 fewer injuries per year and 2,020 fewer healthy years lost-to-injury per year. The estimated high school savings are 143,900 fewer injuries per year and 24,300 …

The Distinct Expressions Of Integrins Αdβ2 And Αmβ2 Differently Regulate Macrophage Migration In 3d Matrix In Vitro And In Tissue During Inflammation, Kui Cui

Electronic Theses and Dissertations

Chronic inflammation is an essential mechanism during the development of cardiovascular and metabolic diseases. The outcome of diseases depends on the balance between the migration and accumulation of macrophages in damaged tissues. Macrophage motility is highly regulated by adhesive receptors, integrins. Namely, intermediate expression of integrin supports macrophage migration, while a high integrin density inhibits it. Our studies are focused on evaluation of the contribution of related integrins α_Dβ₂ and α_Mβ₂ to macrophage migration and development of chronic inflammation.

We found that integrin α_Dβ₂ is upregulated on M1-macrophages in vitro and …

The Evolutionary Diversity And Biological Function Of Phenazine Metabolite Biosynthesis In Burkholderia Spp, Samuel Hendry

Master's Theses

Burkholderia encompass a group of ubiquitous Gram-negative bacteria that include numerous saprophytes, as well as several species that cause infections in animals, immunocompromised patients, and plants. Some species of Burkholderia produce colored redox-active secondary metabolites called phenazines (Phz). In the model opportunistic pathogen Pseudomonas aeruginosa, phenazines strongly contribute to the competitiveness, formation of biofilms, and virulence in multiple models of infection. Similar depth of knowledge on the diversity, biosynthesis, and biological functions of phenazines in Burkholderiais missing. This project aimed to bridge this gap in knowledge by focusing on phenazine pathways of B. lata and closely related …

Financing The World Health Organization: What Lessons For Multilateralism?, Kristina Daugirdas, Gian Luca Burci

Articles

When it comes to financing the work of international organizations, voluntary contributions from both state and nonstate actors are growing in size and importance. The World Health Organization (WHO) is an extreme case from this perspective, with voluntary contributions - mostly earmarked for particular purposes - comprising more than 80 percent of its funds. Moreover, nonstate actors are by now supplying almost half of WHO’s funds, with the Bill and Melinda Gates Foundation ranking as the second-highest contributor after the United States. A number of public-health and international relations scholars have expressed alarm over these trends, arguing that heavy reliance …

Prospects For Clinical Nurse Specialist Education (Mental Health Nursing) At National College Of Nursing, Japan, Makiko Mori

International Nursing Research Congress (INRC)

The specialized course education program enabling the acquisition of the latest techniques and theories for improving the advanced and specialized practical skills of clinical nursing and nursing research skills at the National Advanced Specialist Medical Research Centers and the National Hospitals as well as investigation into the roles and challenges.

Exploring Neighborhood Level Variation In Incidence And Secondary Transmission Of Seasonal Influenza, Melissa Freeland

Masters Theses

Influenza epidemics in the United States, are major causes of morbidity and mortality, and cause serious economic disruption on an annual basis. Evidence has begun to accumulate linking neighborhood-level socioeconomic disadvantage to higher rates of hospitalization and mortality related to influenza (Cordoba & Aiello, 2016; Jung, Lin, & Viswanath, 2013). Some studies suggest that this may be due to lower rates of vaccination, higher rates of comorbidities in socioeconomically disadvantaged communities (Cordoba & Aiello, 2016; Jung, Lin, & Viswanath, 2013; Ross & Mirowsky, 2001). However, few studies have evaluated the effects of neighborhood level socioeconomic disadvantage and structural features on …

Bilirubin Nanoparticle As An Anti-Inflammatory Therapy For Graft Versus Host Disease, Sumedha Pareek

Dissertations and Theses (Open Access)

Graft versus host disease (GvHD) caused by alloreactive donor lymphocytes is a fatal complication of hematopoietic stem cell transplant (HSCT). Myeloablative conditioning regimen, consisting of chemotherapy and/or radiation, given prior to HSCT can cause tissue damage. This non-specific tissue damage triggers cross-presentation of alloantigens to the donor immune cells, causing recruitment of leukocytes and production of inflammatory cytokines. Targeting this inflammation without affecting the anti-leukemia effects of HSCT, continues to be one of the biggest challenge in finding a therapy for GvHD. Bilirubin is a tetrapyrrole pigment, found in the blood, with natural anti-oxidative and anti-inflammatory properties. Using mouse models …

Synthesis, Characterizations, And Study Of The Antioxidant Activity Of Novel Ruthenium-Thione And -Selone Complexes, Mohammed Abdulali Abbas

All Dissertations

Coordination chemistry of imidazole thione and selone compounds with 2nd and 3rd row d-block metals are of considerable interest due to their wide-ranging applications of reducing heavy metal toxicity, catalysis, and antimicrobial and antitumor activity. Antioxidant behavior of some imidazole thiones and selones is attributed to their ability to scavenge hydrogen peroxide or other reactive oxygen species as well as their coordination of redox-active iron and copper to prevent hydroxyl-radical-mediated DNA damage. Chapter 1 of this dissertation provides an overview of 2nd and 3rd row d-block metal complexes with imidazole thione and selone ligands and their applications.

Ruthenium complexes with …

Voices Bearing Witness: Culture And The Birth Process In The District Of Labé, Fouta Djallon, Guinea, Rachel Kristine Lang-Balde

All Dissertations

Every woman has the right to safe motherhood. Over half of the global maternal deaths occur in sub-Saharan Africa. In Guinea, where this study took place, a woman has a one-in-26 chance of dying in childbirth, compared to a one-in-1,400 in a developed nation. Thus, the purpose of this study was to identify the characteristics and the role of cultural beliefs and practices on a woman's birth process (conception to post-partum) among the Fulani in the Labé district of the Fouta Djallon region in Guinea using qualitative and participatory methods and collaborating with those most impacted by this issue, the …

Temperature Influence On Insecticide Resistance In Aedes Aegypti And Aedes Albopictus Mosquitoes From South Texas, Wendy Soledad Salinas

Theses and Dissertations

Aedes aegypti and Aedes albopictus are vectors for several emerging arboviruses including Zika, chikungunya and dengue. Both mosquitoes are found along the Rio Grande River in South Texas, along the border between Mexico and the United States of America. These mosquito species typically reside near human settlements. A preventative measure public health official use to help halt the spread of diseases is by controlling vector population with the use of insecticides, but as temperature vary, and with the constant exposure to commonly used insecticides, mosquitoes from South Texas may be developing a resistance to the insecticides. Resistance to insecticides may …

Novel Cell Adhesion/Migration Pathways Are Predictive Markers Of Hdac Inhibitor Resistance In Cutaneous T Cell Lymphoma, Jared M Andrews, Jennifer A Schmidt, Kenneth R Carson, Amy C Musiek, Neha Mehta-Shah, Jacqueline E Payton

Open Access Publications

BACKGROUND: Treatment for Cutaneous T Cell Lymphoma (CTCL) is generally not curative. Therefore, selecting therapy that is effective and tolerable is critical to clinical decision-making. Histone deacetylase inhibitors (HDACi), epigenetic modifier drugs, are commonly used but effective in only ~30% of patients. There are no predictive markers of HDACi response and the CTCL histone acetylation landscape remains unmapped. We sought to identify pre-treatment molecular markers of resistance in CTCL that progressed on HDACi therapy.

METHODS: Purified T cells from 39 pre/post-treatment peripheral blood samples and skin biopsies from 20 patients were subjected to RNA-seq and ChIP-seq for histone acetylation marks …

Host And Viral Mechanisms Of Congenital Zika Syndrome, Brooke Liang, José Paulo Guida, Maria Laura Costa Do Nascimento, Indira U Mysorekar

Open Access Publications

In 2015-2016, in the Americas, and especially in northeast Brazil, a significant number of cases of microcephaly and other congenital brain abnormalities were linked with an outbreak of Zika virus (ZIKV) infection in pregnant women. While maternal symptoms of ZIKV are generally mild and self-limiting, clinical presentation in fetuses and newborns infected is extensive and includes microcephaly, decreased cortical development, atrophy and hypoplasia of the cerebellum and cerebellar vermis, arthrogryposis, and polyhydramnios. The term congenital ZIKV syndrome (CZS) was introduced to describe the range of findings associated with maternal-fetal ZIKV transmission. ZIKV is primarily transmitted by

Association Of Copy Number Variations With Chronic Hepatitis B In Chinese Population, Fang Niu

Capstone Experience: Master of Public Health

With one third of the Hepatitis B virus (HBV) infection population of the world, chronic Hepatitis B (CHB) has become a top burden in China. CHB is a lifelong infection with HBV which can cause serious health problems, like cirrhosis, liver cancer or even death. HBV infection is known to result in various clinical conditions, including asymptomatic HBV carriers to chronic hepatitis and primary hepatocellular carcinoma. Several studies have shown that host genetic susceptibility could be an important factor that determines these various outcomes of HBV infection. Many Single Nucleotide Polymorphisms (SNPs) and Copy Number Variations (CNVs) have been associated …