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Exploring The Capacity Of Bacteria For Natural Product Biosynthesis, Ozkan Fidan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This dissertation is focused on exploring the potential of bacteria for the biosynthesis of natural products with the purposes of generating novel natural product derivatives and of improving the titer of pharmaceutically important natural products.

A wide variety of compounds from various sources have been historically used in the treatment and prevention of diseases. Natural products as a major source of new drugs are extensively explored due to their huge structural diversity and promising biological activities such as antimicrobial, anticancer, antifungal, antiviral and antioxidant properties. For instance, penicillin as an early-discovered antimicrobial agent has saved millions of lives, indicating the …

Biophysical And Structural Characterization Of Shigella Atpase Spa47 Oligomerization Provides Insight Into Type Three Secretion System Activation And Virulence, Jamie L. Burgess

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Several bacterial pathogens including Shigella (shigellosis), Escherichia coli (urinary tract infections), Pseudomonas (lung infections), Salmonella (food poisoning), and Yersinia (plague) critically rely on a complex type three secretion system (T3SS) for infection. With the rise in multi-antibiotic resistant strains of several of these pathogens, we turn to the T3SS as a promising target for the development of novel therapeutics. The Dickenson lab at Utah State University has been the first to identify and characterize the ATPase Spa47, the energy source of the Shigella infection system. We show that Spa47 is necessary for proper T3SS formation and function, being ultimately responsible …

Usf Women's Club Yearbook, 2019–2020, Usf Women's Club

USF Women’s Club Collection

Yearbook of the USF Women's Club. Inside is a list of past presidents, officers, committee chairmen, and members for 2019–2020. The booklet also includes a tribute to Grace Allen, interest groups, calendar of events, and a copy of the constitution and by-laws from the handbook.

Control Of Infectious Diseases In A Metapopulation, Ceyda Best

All Dissertations

With the motivation of the complex infectious disease control problem, we provide two different approaches to model the resource allocation problem to control an epidemic in a metapopulation. All of our models utilize a detailed stochastic simulation model that is validated with the data from the 2014 Ebola epidemic. This simulation model provides a tool for comparing the performance of different policies.

The first model defines a dynamic allocation problem, which is modeled by a Markov Decision Process, and aims to find feasible and effective quarantine policies to control an epidemic with limited resources. We assume that the populations share …

Understanding The Molecular Strategies Of Campylobacter Jejuni For Survival In Amoeba And Chicken., Deepti Pranay Samarth

Graduate Theses and Dissertations

Campylobacter jejuni endure to be major cause of gastroenteritis in humans worldwide. C. jejuni is fastidious in laboratory setup but can cause waterborne infection through contaminated water where none of these fastidious conditions are met. This dissertation presents an assortment of studies focused in reviewing three major factors which could present a helping hand to C. jejuni in its environmental survival viz. i) association with free-living amoebae (FLA) ii) horizontal gene transfer (HGT) contributing towards its genetic diversity iii). Viable but non-culturable (VBNC) state.

Acanthamoeba is a FLA linked to environmental survival of many intracellular pathogens, including C. jejuni. In …

Illinois State, August 2019, Illinois State University, University Marketing And Communications

State: The Magazine of Illinois State University (1999-present)

Illinois State University alumni magazine

Periodontal Pathogens Are A Risk Factor Of Oral Cavity Squamous Cell Carcinoma, Independent Of Tobacco And Alcohol And Human Papillomavirus, Ian Ganly, Liying Yang, Rachel Giese, Yuhan Hao, Carlos W. Nossa, Luc G. T. Morris, Matthew Rosenthal, Jocelyn Migliacci, Dervla Kelly, Wenzhi Tseng

School of Medicine Publications

Over the past decade, there has been a change in the epidemiology of oral cavity squamous cell cancer (OC-SCC). Many new cases of OC-SCC lack the recognized risk factors of smoking, alcohol and human papilloma virus. The aim of this study was to determine if the oral microbiome may be associated with OC-SCC in nonsmoking HPV negative patients. We compared the oral microbiome of HPV-negative nonsmoker OC-SCC(n = 18), premalignant lesions(PML) (n = 8) and normal control patients (n = 12). Their oral microbiome was sampled by oral wash and defined by 16S rRNA gene sequencing. We report that the …

Heat Waves And Heat-Related Mortality In East Tennessee, Taiwo Adesoba

Electronic Theses and Dissertations

Heat waves represent a public health challenge that requires multiple responses and warnings to protect vulnerable populations. Although studies have reported an increasing trend of heat wave occurrence in many areas of the world, no clear trend exists in East Tennessee. Using data from Parameter-elevated Relationships on Independent Slope Models (PRISM), CDC WONDER and the United States Census Bureau, the relationship between mortality rates and year was estimated during heat wave events between 1999 and 2010. Five heat wave definitions were tested. Overall, 2007 and 2010 stand out as the years with the highest number of heat wave days in …

Reducing Central Line-Associated Bloodstream Infections In The Intensive Care Unit By Improving Compliance With Dressing Changes And Curos Caps Use, Jennifer Khaziga Kilel

Doctoral Dissertations and Projects

Background: Reducing central line-associated bloodstream Infections (CLABSI) is a topic of global importance due to associated risk of sepsis, increased length of hospital stay, high healthcare cost, and increased morbidity and mortality among critically ill patients in the intensive care unit (ICU). The mortality rate of patients who acquire CLABSI 15-25% The cost of CLABSI treatment per event is greater than US $16,500. Central cine-associated Bloodstream Infections affect the organization’s national ranking in patient quality and safety as well as reimbursement from the Centers for Medicare and Medicaid (CMS). The aim of the quality improvement project was to improve the …

Rosai-Dorfman's Disease, An Uncommon Cause Of Common Clinical Presentation, Om Parkash, Mian Shah Yousaf, Ghulam Fareed

Section of Gastroenterology

Sinus histiocytosis with massive lymphadenopathy also known as Rosai-Dorfman disease is a rare benign disease that typically manifests as lymphadenopathy with or without systemic manifestations whose etiology remains poorly understood. Most common clinical presentation is painless bilateral cervical lymphadenopathy. However, it also can present in various extranodal sites and can easily be missed because of its rarity if not considered in the differential diagnosis. It commonly occurs in children and young adults with a slightly male predominance. Clinically patients may be mistaken for lymphoma and other infectious disorders like tuberculosis especially in developing countries like in Pakistan where tuberculosis is …

Correlates Of Same-Sex Behavior Disclosure To Health Care Providers Among Black Msm In The United States: Implications For Hiv Prevention, Christina J. Sun, Karin Tobin, Pilgrim Spikes, Carl Latkin

OHSU-PSU School of Public Health Faculty Publications and Presentations

Disclosure of same-sex behavior to health care providers (HCPs) by men who have sex with men (MSM) has been argued to be an important aspect of HIV prevention. However, Black MSM are less likely to disclose compared to white MSM. This analysis of data collected in the United States from 2006–2009 identified individual and social network characteristics of Black MSM (n = 226) that are associated with disclosure that may be leveraged to increase disclosure. Over two-thirds (68.1%) of the sample had ever disclosed to HCPs. Part-time employment (AOR = 0.32, 95% CI = 0.11–0.95), bisexual identity (AOR = …

Clinical Practice Variation Among Adult Infectious Disease Physicians In The Management Of Staphylococcus Aureus Bacteremia, Catherine Liu, Luke Strnad, Susan E. Beekmann, Philip M. Polgreen, Henry F. Chambers

OHSU-PSU School of Public Health Faculty Publications and Presentations

Infectious disease management of Staphylococcus aureus bacteremia (SAB) was surveyed through the Emerging Infections Network. While there were areas of consensus, we found substantial practice variation in diagnostic evaluation and management of adult patients with SAB. These findings highlight opportunities for further research and guidance to define best practices.

Ruminant Bloat, Austin Sears, Kerry A. Rood

All Current Publications

This fact sheet describes bloat, a non-infectious disease common among the ruminant species, including types and clinical signs, prevention, and treatment.

Intervention Principles In Pediatric Health Care: The Difference Between Physicians And The State., D. Robert Macdougall

Publications and Research

According to various accounts, intervention in pediatric decisions is justified either by the best interests standard or by the harm principle. While these principles have various nuances that distinguish them from each other, they are similar in the sense that both focus primarily on the features of parental decisions that justify intervention, rather than on the competency or authority of the parties that intervene. Accounts of these principles effectively suggest that intervention in pediatric decision making is warranted for both physicians and the state under precisely the same circumstances. This essay argues that there are substantial differences in the competencies …

How Acceptable Is The Hiv/Aids Self-Testing Among Women Attending Immunization Clinics In Effurun, Southern Nigeria, Wasiu Olalekan Adebimpe, Doraebikeme Ebikeme, Olubukunola Omobuwa, Edward Oladejo

Marshall Journal of Medicine

HIV testing remains the gateway to HIV/AIDS prevention, treatment, care and support interventions. In Nigeria, a significant proportion of the populations do not know their HIV status. HIV self-testing done without the help of a healthcare provider could remove identified barriers to HIV testing, and close gaps in HIV treatment and prevention cascades. This study set out to assess the knowledge and acceptability of hiv self-testing (HIVST) among women of child bearing age attending immunization clinics in Effurun, Nigeria. A descriptive, cross-sectional study was conducted among 357 women of child-bearing age selected using multistage sampling technique. Research instrument used was …

Group A Streptococcus Brain Abscess In A Patient On Adalimumab Therapy, Ryan Carroll, Ahmad Abu-Hashyeh, James Gainer, Kara Willenburg, Charles Meadows

Marshall Journal of Medicine

Brain abscesses represent a group of dangerous infections typically caused by a number of classic organisms. Group A Streptococcus is a rare cause of brain abscesses and those patients that do have this particular condition will typically have a distal source of infection. We present a case of patient with a biopsy confirmed Group A Streptococcal without a distal source of infection. His only identifiable risk factor was his adalimumab therapy for ankylosing spondylitis. While adalimumab has not been linked to Streptococcal infection in the past, it has been associated with multiple atypical infections and some of the consequences of …

2019 Student Research Fellowship Program: Serf Abstract Booklet, Andy Alvarez Bs, Deborah Mcmahan Md, Lisa Hollister Msn, Rn, Thein Zhu Mbbs, Face, Frcp, Julie Hughbanks, Annette Chard Rn, Cen, Jana Sanders M. En, Grayson Bastin, Aaron Roberts Md, Kristopher D. Bosi, Jui-Hung Yen Phd, Sergio Steven Cobos Ba, Nancy Connelly Rn, Bsn, Jeffrey Nickel Md, Cameron Duffner Bs, Uchechukwu Emili Bs, Heather Wolfe Md, Denise Gilham Np, Susan Stace Rn, Cpn, Mitchell Gray Ba, Kyle Davis Bs, Lauren Jagger, Micah Smith Md, Christopher Herrera Bs, Douglas Gray Md, Kim Recht Np, Conner T. Holcomb Bs, Adam D. Hall Md, Brandon Kiley Bs, T. Eric White Md, Emily Keltner Bs, Ma, Dazar Opoku Mph, Luke Landrigan Bs, John Lozo Bs, Randall Mccomb Ms, Kent Redman Phd, Haley Moon Ms, Alexia Parra Ms, Hollace Chastain Md, Brycen Ratcliffe Bs, Candace Rogers Rn, Scott Stienecker Md, Taylor Schaetzle Bs, Fenlei Chang Md, Phd, Samuel Stegelmann Bs, William Young Md, Daniel Torolira Bs, Sara Brown Md, William Voors Bs, Abhishek Biswas Md, Facp, Andy Wang, Takefumi Komiya Md

Medical Student Research

Indiana University School of Medicine - 2019 Abstracts:

Are Trauma Centers in Northeast Indiana Prepared in Triage for Mass Casualty Events?

Severity of Obstructive Sleep Apnea: Patient Compliance and Treatment Response.

Interferon-β treatment to prolong t-PA treatment window in cerebral ischemia.

Quantitative Effect of Emergency Department Case Management on Visits, Diagnostics, and Cost.

Neonatal Follow Up Clinic: A Retrospective Chart Review of Developmental Outcomes.

Comparison of Technical Outcomes in Instrumented Posterolateral Fusion (PLF) with and without Transforaminal Lumbar Interbody Fusion (TLIF) performed using Silicon Nitride cages.

Long Term Outcomes of Iliac Artery Aneurysm (IAA) and Abdominal Aortic Aneurysm (AAA) Repair …

Development Of Novel Zika And Anthrax Viral Nanoparticle Vaccines, Elizabeth Henderson

KGI Theses and Dissertations

Vaccines protect against numerous infectious diseases and prevent millions of deaths annually, but there are still many infectious diseases for which no licensed vaccine exists. Developing a new vaccine requires balancing safety and efficacy, and viral nanoparticle (VNP) vaccines possess both of these characteristics. The work herein demonstrates how tobacco mosaic virus (TMV) nanoparticles can serve as a platform to create candidate vaccines for Zika virus (ZIKV) and anthrax. In the first study, a ZIKV-specific epitope was genetically fused to TMV to create a safe and inexpensive vaccine that proved highly immunogenic in mice and led to the discovery of …

Plasticity In The Human Gut Microbiome Defies Evolutionary Constraints, Andres Gomez, Ashok Kumar Sharma, Elizabeth K. Mallott, Klara J. Petrzelkova, Carolyn A. Jost Robinson, Carl J. Yeoman, Franck Carbonero, Barbora Pafco, Jessica M. Rothman, Alexander Ulanov, Klara Vickova, Katherine R. Amato, Stephanie L. Schnorr, Nathaniel J. Dominy, David Modry, Angelique Todd, Manolito Torralba, Karen E. Nelson, Michael B. Burns, Ran Blekhman, Melissa Remis, Rebecca M. Stumpf, Brenda A. Wilson, H. Rex Gaskins, Paul A. Garber, Bryan A. White, Steven R. Leigh

Anthropology Faculty Research

The gut microbiome of primates, including humans, is reported to closely follow host evolutionary history, with gut microbiome composition being specific to the genetic background of its primate host. However, the comparative models used to date have mainly included a limited set of closely related primates. To further understand the forces that shape the primate gut microbiome, with reference to human populations, we expanded the comparative analysis of variation among gut microbiome compositions and their primate hosts, including 9 different primate species and 4 human groups characterized by a diverse set of subsistence patterns (n = 448 samples). The results …

Migration And Mental Health In The Aftermath Of Disaster: Evidence From Mt. Merapi, Indonesia, Jonathan A. Muir, Michael R. Cope, Leslie R. Angeningsih, Jorden E. Jackson, Ralph B. Brown

Faculty Publications

Migration is a standard survival strategy in the context of disasters. While prior studies have examined factors associated with return migration following disasters, an area that remains relatively underexplored is whether moving home to one’s original community results in improved health and well-being compared to other options such as deciding to move on. In the present study, our objective is to explore whether return migration, compared to other migration options, results in superior improvements to mental health. We draw upon data from a cross-sectional pilot study conducted 16 months after a series of volcanic eruptions in Merapi, Indonesia. Using ordinal …

The Effectiveness Of Geographic Information System Training For Surveillance Officers In Sukabumi District, Martya Makful

ASEAN Journal of Community Engagement

Surveillance is the systematic, continuous collection, processing, analysis and interpretation of data and the dissemination of information to units in need to be able to take action. In the field of health, human resources (HR) in an organization is very important role. Because the quality of health services to the public is determined by the human resources working in it. With the number of reports and cases of outbreaks in Sukabumi district, it is necessary a system of information and recording of data that muktahir. The purpose of this research is to know the effectiveness of training of geographic information …

Validation Of A Death Assay For Angiostrongylus Cantonensis Larvae (L3) Using Propidium Iodide In A Rat Model (Rattus Norvegicus), Susan I. Jarvi, John Jacob, Robert T. Sugihara, Israel L. Leinbach, Ina H. Klasner, Lisa M. Kaluna, Kirsten A. Snook, M. Kathleen Howe, Steven H. Jacquier, Ingo Lange, Abigail L. Atkinson, Ashley R. Deane, Chris N. Niebuhr, Shane R. Siers

United States Department of Agriculture Wildlife Services: Staff Publications

Angiostrongylus cantonensis is a pathogenic nematode and the cause of neuroangiostrongyliasis, an eosinophilic meningitis more commonly known as rat lungworm disease. Transmission is thought to be primarily due to ingestion of infective third stage larvae (L3) in gastropods, on produce, or in contaminated water. The gold standard to determine the effects of physical and chemical treatments on the infectivity of A. cantonensis L3 larvae is to infect rodents with treated L3 larvae and monitor for infection, but animal studies are laborious and expensive and also raise ethical concerns. This study demonstrates propidium iodide (PI) to be a reliable marker of …

Designing Development Programs For Non-Traditional Antibacterial Agents, John H. Rex, Holly Fernandez Lynch, I. Glen Cohen, Jonathan J. Darrow, Kevin Outterson

Faculty Scholarship

In the face of rising rates of antibacterial resistance, many responses are being pursued in parallel, including ‘non-traditional’ antibacterial agents (agents that are not small-molecule drugs and/or do not act by directly targeting bacterial components necessary for bacterial growth). In this Perspective, we argue that the distinction between traditional and nontraditional agents has only limited relevance for regulatory purposes. Rather, most agents in both categories can and should be developed using standard measures of clinical efficacy demonstrated with non-inferiority or superiority trial designs according to existing regulatory frameworks. There may, however, be products with non-traditional goals focused on population-level benefits …

Designing The Arriving Refugee Informatics Surveillance And Epidemiology (Arive) System: A Web-Based Electronic Database For Epidemiological Surveillance, William A. Mattingly, Ruth M. Carrico, Timothy L. Wiemken, Robert R. Kelley, Rebecca A. Ford, Rahel Bosson, Kimberley A. Buckner, Julio A. Ramirez

Journal of Refugee & Global Health

Objectives: We design and implement the Arriving Refugee Informatics surVeillance and Epidemiology (ARIVE) system to improve the health of refugees undergoing resettlement and enhance existing health surveillance networks.

Materials and Methods: Using the REDCap electronic data capture software as a basis we create a refugee health database incorporating data from the Center for Disease Control and Prevention’s Electronic Disease Notification (EDN) system and domestic screening data from refugee health care providers.

Results: Domestic screening and EDN refugee health data have been integrated for 13,824 refugees resettled from 35 different countries into the state of Kentucky from the years 2013-2016.

Discussion: …

Scientific Twitter: The Flow Of Paleontological Communication Across A Topic Network, Richard T. Bex Ii, Lisa Lundgren, Kent J. Crippen

Instructional Technology and Learning Sciences Faculty Publications

The field of paleontology, which is based principally on observations of the natural world, includes an active community that is engaged across multiple social media platforms, consisting of museums, academic researchers, amateur fossil collectors, paleontological artists, and commercial fossil dealers. As such, it represents an ideal environment for examining the people, interactions, and flow of scientific information. Using interactions involving the four most popular Twitter hashtags for paleontology, this embedded mixed methods study defined the members of this social world and investigated how they influenced and controlled the flow of information, as well as how their expression of scientific practice …

Implementation Of Control Policy Of Diphtheria Outbreak And Outbreak Response Immunization (Ori) In Kota Depok, 2017, Dian Novita Sari, Anhari Achadi

Journal of Indonesian Health Policy and Administration

Diphtheria is a contributor to an outbreak (KLB) for some regions in Indonesia, included West Java Province. Diphtheria cases had increased through 2015 - 2016, the cases increased from 59 cases to 153 cases. Depok City became one of the contributors of diphtheria cases that have fluctuating incidents. Disease trends have decreased in 2013-2015 but then increased in 2016 to 8 cases. This study discusses policy implementation, viewed from policy sources, resource arrangements, the characteristics of implementing agencies, bureaucratic structures, communications, the influence of dispositions and socio-economic and political circumstances in control of Diphtheria Outbreak and Outbreak Response Immunization (ORI) …

Engaging Crisis: Immersive, Interdisciplinary Learning In Mathematics And Rhetoric, Meredith L. Greer, Stephanie Kelley-Romano

Journal of Humanistic Mathematics

This paper describes an interdisciplinary activity that crosses over between Mathematics and Rhetoric. The professors who created this activity both sought active-learning opportunities for their students, a sense of realism--even urgency--in what can otherwise be perceived as abstract material, and a meaningful liberal arts experience. Evidence of the power of this experience is seen in the media coverage, both from our college and from the Portland Press Herald newspaper. Both courses described in this paper are at the elective level, taken by majors or minors in their respective disciplines. Students have moderate to extensive backgrounds in their subject areas. However, …

Combating Tuberculosis: Using Time-Dependent Sensitivity Analysis To Develop Strategies For Treatment And Prevention, Kendall B. Clark, Mayleen Cortez, Cristian Hernandez, Beth E. Thomas, Allison L. Lewis

Spora: A Journal of Biomathematics

Although many organizations throughout the world have worked tirelessly to control tuberculosis (TB) epidemics, no country has yet been able to eradicate the disease completely. We present two compartmental models representing the spread of a TB epidemic through a population. The first is a general TB model; the second is an adaptation for regions in which HIV is prevalent, accounting for the effects of TB/HIV co-infection. Using active subspaces, we conduct time-dependent sensitivity analysis on both models to explore the significance of certain parameters with respect to the spread of TB. We use the results of this sensitivity analysis to …

Equine Arteritis Virus Long-Term Persistence Is Orchestrated By Cd8+ T Lymphocyte Transcription Factors, Inhibitory Receptors, And The Cxcl16/Cxcr6 Axis, Mariano Carossino, Nikolaus Osterrieder, Pouya Dini, Theodore S. Kalbfleisch, Alan T. Loynachan, Igor F. Canisso, R. Frank Cook, Peter J. Timoney, Udeni B. R. Balasuriya

Faculty Publications

No abstract provided.

Emergency Care Surveillance And Emergency Care Registries In Low-Income And Middle-Income Countries: Conceptual Challenges And Future Directions For Research, Hani Mowaf, Christine Ngaruiya, Gerard O'Reilly, Olive Kobusingye, Vikas Kapil, Andres Rubiano, Marcus Ong, Juan Carlos Puyana, Akm Fazlur Rahman, Rashid Jooma, Blythe Beecrof, Junaid Razzak

Section of Neurosurgery

Despite the fact that the 15 leading causes of global deaths and disability-adjusted life years are from conditions amenable to emergency care, and that this burden is highest in low-income and middle-income countries (LMICs), there is a paucity of research on LMIC emergency care to guide policy making, resource allocation and service provision. A literature review of the 550 articles on LMIC emergency care published in the 10-year period from 2007 to 2016 yielded 106 articles for LMIC emergency care surveillance and registry research. Few articles were from established longitudinal surveillance or registries and primarily composed of short-term data collection. …