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Speech-Language-Hearing Clinic Handbook - August 2019, Department Of Audiology And Speech-Language Pathology, East Tennessee State University

Speech-Language Pathology Handbooks

No abstract provided.

Heat Activation Of Bacillus Anthracis Persists Over A Seven Day Period, Nathan T. Ross

Master's Theses

Bacteria of the Bacillus genera are capable of forming dormant and resilient cells called spores in response to starvation. Spores can break dormancy in the presence of nutrients in a process called germination. Historically, spores are exposed to sublethal heat treatments to increase the extent and rate of germination. This process is known as heat activation (HA). Previous studies on Bacillus species indicate the effects of HA are reversible after 72 hours. After this time period, the spores must be reactivated. However, spores of Bacillus anthracis, a member of the Bacillus family, have not been tested to see how long …

Out-Of-Pocket Expenditure For Home And Facility Based Delivery Among Rural Women In Zambia: A Mixed-Methods, Cross-Sectional Study, Jeanette L. Kaiser, Kathleen L. Mcglasson, Peter C. Rockers, Rachel M. Fong, Thandiwe Ngoma, Davidson H. Hamer, Taryn Vian, Godfrey Biemba, Jody R. Lori, Nancy A. Scott

Nursing and Health Professions Faculty Research and Publications

Purpose: Out-of-pocket expenses associated with facility-based delivery are a well-known barrier to health care access. However, there is extremely limited contemporary information on delivery-related household out-of-pocket expenditure in sub-Saharan Africa. We assess the financial burden of delivery for the most remote Zambian women and compare differences between delivery locations (primary health center, hospital, or home).

Methods: We conducted household surveys and in-depth interviews among randomly selected remote Zambian women who delivered a baby within the last 13 months. Women reported expenditures for their most-recent delivery for delivery supplies, transportation, and baby clothes, among others. Expenditures were converted to …

Natural Autoantibodies: Origin, Function And Utility For Diagnosis Of Disease, Abhirup Sarkar

Graduate School of Biomedical Sciences Theses and Dissertations

Autoantibodies (aAbs) by the simplest definitions have been described as antibodies against self-antigens and were exclusively associated with autoimmune diseases. Eventually, studies demonstrated that they are abundant in the blood of all human sera, regardless of age, gender, or the presence or absence of disease, and were thus named as ‘natural autoantibodies’. The underlying reason for their ubiquity has remained elusive, but we have hypothesized that they are responsible for clearing blood-borne cell and tissue debris generated under conditions of health and disease. To test this, we chose to use two widely different disease model systems, namely neurodegenerative diseases and …

Emergence Of Human Immunoprofiling In Health And Disease, Bhagirath Singh

Microbiology & Immunology Publications

The human immune system is critical for maintaining health and providing protection from infectious diseases and cancer. Major advances in our understanding of the immune system have largely emerged from studies using animal models such as mice. However, this mouse-centric research has also limited our ability to comprehend the human immune system and how it changes with age and disease state. The fact that we have yet to define what constitutes a normal human immune system has hampered our ability to diagnose, treat, and prevent many human diseases. Immunoprofiling that measures the frequency of human immune cells based upon their …

An Educational Intervention On Daily Chlorhexidine Gluconate Bathing And Central Venous Catheter Infection Rates In The Pediatric Population: A Quality Improvement Initiative., Jennifer Cothern

Doctor of Nursing Practice Papers

The use of Central Venous Catheters (CVCs) in the healthcare setting is essential to patient survivability. While convenient, the utilization of such devices places patients at risk for Central Line- Associated Bloodstream Infections (CLABSI). The US spends nearly $2 billion dollars annually treating CLABSIs. The utilization of CLABSI bundles has shown to be effective in infection prevention. Daily bathing with Chlorhexidine Gluconate (CHG) is recommended by the Centers for Disease Control and Prevention (CDC) and is often included in CLABSI bundles. In an attempt to reduce the incidence of CLABSIs at a metropolitan pediatric hospital in the Southern US, a …

Leaving Against Medical Advice From In-Patients Departments Rate, Reasons And Predicting Risk Factors For Re-Visiting Hospital Retrospective Cohort From A Tertiary Care Hospital, Obada Hasan, Muhammad Adil Samad, Hamza Khan, Maryam Sarfraz, Shahryar Noordin, Tashfeen Ahmad, Gul Nowshad

Section of Orthopaedic Surgery

Background: Approximately 1% to 2% of hospitalized patients get discharged or leave from the hospital against medical advice and up to 26% in some centers. They have higher readmission rate and risk of complications than patients who receive complete care. In this study we aimed to determine the rate of leave against medical advice (LAMA) and reasons for the same across different in-patient departments of a tertiary care hospital.
Methods: Retrospective cohort study on patients admitted in all departments at our institute over a 1-year period. All patients who were admitted to an in-patient ward at the hospital and who …

Knowledge Of Women Of Childbearing Age Regarding Preconception Counseling, Allison Britt, Shelby Jackson, Jessica Mcgee, Jenna Pugh

MSN Research Projects

The purpose of the current research study was to assess the knowledge of women of childbearing age regarding key components of preconception counseling and look at relationships between demographics and knowledge of preconception health care.

Amphibious Entrepreneurs And The Origins Of Invention, Kurt Sandholtz, Walter W. Powell

Faculty Publications

In this chapter, we examine entrepreneurs who carry ideas, technologies, values, and assumptions between previously unrelated spheres of economic or cultural activity, and in the process, change the existing order of things. We label such individuals amphibious entrepreneurs and explore their characteristics via four case studies. Their stories suggest a distinct species within the genus of entrepreneur: more pragmatic than heroic, and as likely to invent by not knowing any better as by calculative creation. We discuss their role in creating interstitial spaces, contrast them to other boundary-spanning actors, and identify directions for future research at the intersection of social …

Evaluation Of Data Collection Operations For Real-Time Influenza Surveillance During An Emergency, Yuwen Gu

Dissertations

It is unclear how data collection operations for surveillance alter the disease portrayal that influenza reported trends attempt to provide during an emergency. This study developed a model that simulates the collection and testing of influenza specimens after an outbreak is declared in Michigan. It performed simulation based optimization to understand which operational factors affect the biases between the growth rates of original and observed influenza incidence trends, and to quantify the predictive power of the influenza incidence trends at different points of data collection. The results show that emergency driven high risk perception increases the reporting, which leads to …

Zika-Related Knowledge, Attitudes, And Practices: Programmatic Implications For Zika Prevention In The Dominican Republic, Breakthrough Research

Poverty, Gender, and Youth

This brief provides key insights for implementers of social and behavior change (SBC) activities for Zika prevention in the Dominican Republic. The insights are based on data from a representative sample of people living in select USAID Zika program implementation communities and support an evidence-based approach for programming to increase preventative behaviors for Zika and other Aedes aegypti-transmitted viruses. The data indicate a need to narrow existing knowledge gaps related to the Zika virus, particularly its health consequences and effective preventive practices, and communicate more specifically about preventative behaviors to enable effective action.

Early Progression To Active Tuberculosis Is A Highly Heritable Trait Driven By 3q23 In Peruvians, Yang Luo, Sara Suliman, Samira Asagari, Tiffany Amariuta, Yuriy Baglaenko, Marta Martinez-Bonet, Kazuyoshi Ishigaki, Maria Gutierrez-Arcelus, Roger Calderon, Jerome Galea

Social Work Faculty Publications

Of the 1.8 billion people worldwide infected with Mycobacterium tuberculosis, 5–15% will develop active tuberculosis (TB). Approximately half will progress to active TB within the first 18 months after infection, presumably because they fail to mount an effective initial immune response. Here, in a genome-wide genetic study of early TB progression, we genotype 4002 active TB cases and their household contacts in Peru. We quantify genetic heritability h_g² of early TB progression to be 21.2% (standard error 0.08). This suggests TB progression has a strong genetic basis, and is comparable to traits with well-established genetic bases. We …

Southeast Asia: A Potential Domain For Chemical Terrorism, Bryner Ramos Las

Graduate Theses/Dissertations

This research examines the prospect of Southeast Asia as a future domain for chemical terrorism over the next five years by assessing the correlation between the intent and capability of potential actors to conduct chemical terrorism. Additionally, the effectiveness of prevention and mitigation measures of the concerned governments is also examined. Southeast Asian countries have experienced the persistent threat of Islamic violent extremism for decades. The exploitation of chemical weapons (CW) reflects new developments in the methods of committing terrorist acts. Al Qaeda and the Islamic State of Iraq and Syria (ISIS) have demonstrated the intent and capability of chemical …

Elucidating Immune Signaling Of Influenza A Virus And Aspergillus Fumigatus Co-Infections Through Pioneered Model Development, Meagan Danyelle Rippee-Brooks

Graduate Theses/Dissertations

Bacterial co-infections with influenza A virus (IAV) are extremely serious and life-threatening. However, there exists limited understanding about the importance of fungal infections with IAV. Clinical case reports indicate that fungal co-infections do occur and suggest the IAV pandemic of 2009 had a propensity to predispose patients to secondary fungal infections more than previous IAV strains. IAV-fungal co-infections are marked by high mortality rates of 47 to 61% in previously healthy individuals between the ages of 20 and 60. Yet, the variables involved in this co-infection remain undetermined. I achieved effective recapitulation of this co-infection using a C57Bl/6 murine (mouse) …

Suppressor Of Cytokine Signaling-3 In Pregnant Females With Or Without Hypertension, Huma Shoukat Ali, Mubeen Ali, Sabah Farhat, Syeda Sadia Fatima

Department of Biological & Biomedical Sciences

Suppression of Cytokine Signalling-3 (SOCS-3) modulates the inflammatory pathways responsible for vascular stability. Therefore, we aimed to estimate SOCS-3 levels in 2nd trimester pregnant females and correlate it with blood pressure. A case control study recruiting (n=111) females was conducted at the Aga Khan University. They were classified as pregnancy induced hypertensives ornormotensive as per American College of Obstetricians and Gynecologists Guidelines. Weight, Body mass index, lipid profile and blood glucose were recorded while SOCS-3 was measured by ELISA. Higher SOCS-3 levels were seen in hypertensive group (30 pg/ml) versus normotensive (16 pg/ml). Both Systolic & diastolic blood pressure (r=0.520; …

Salem Belles, Succubi, And The Scarlet Letter: Transatlantic Witchcraft And Gothic Erotic Affect, Sylvia Cutler

Theses and Dissertations

In order to reconcile the absence of sexually deviant witch figures (succubae, demonic women, etc.) within the formation of American national literature in the nineteenth century with the fantastic elements found in European variations on the gothic, my thesis aims to demonstrate transatlantic variants of erotic signifiers attached to witch figures in nineteenth-century gothic fiction and mediums across national traditions. I will begin by tracing the transatlantic and historical impact of Heinrich Kramer and Jacob Sprenger’s Malleus Maleficarum—an early modern handbook of sorts used widely in witchcraft inquisitions—on Early American witch trials, specifically where its influence deviates from a sexualized …

Protection Against Neutrophil Extracellular Trap (Net) Toxicity By Antioxidant Monoher, Puji Astuti, Danielle M A Beurskens, Tanja Vajen, Gerry A F Nicolaes, Ming Zhang, Guido R M M Haenen

Makara Journal of Health Research

Background: Neutrophil extracellular traps (NET) are extracellular fibers produced by activated neutrophils to kill bacteria. NET was recently found to be associated with several diseases, such as autoimmune diseases. NET formation, called NETosis, is reactive oxygen species (ROS) dependent, thereby, prompting us to study its inhibition by potent antioxidant monoHER as well as to study monoHER protection against NET toxicity caused by NET constituent histone 3 on endothelial cells. Methods: Freshly isolated neutrophils from male donors were stimulated with PMA to induce NET formation. The effect of monoHER (50 µM) on oxidative burst (O2●− production) and NET formation was determined …

Longitudinal Quantification Of Adenovirus Neutralizing Responses In Zambian Mother-Infant Pairs: Impact Of Hiv-1 Infection And Its Treatment, Sara R. Privatt, Brianna L. Bullard, Eric A. Weaver, Charles Wood, John T. West

Nebraska Center for Virology: Faculty Publications

Vaccination offers the most cost-effective approach to limiting the adverse impact of infectious and neoplastic diseases that reduce the quality of life in sub-Saharan Africa (SSA). However, it is unclear what vaccine vectors would be most readily implementable in the setting and at what age they should be applied for maximal efficacy. Adenoviruses (Ad) and Ad-based vectors have been demonstrated to induce effective humoral and cellular immune responses in animal models and in humans. However, because immunity associated with Ad infection is lifelong, there exists a debate as to whether pre-existing immunity might decrease the efficacy of Ad vectored vaccines. …

The Dynamism Of Treaties, Yanbai Andrea Wang

Maryland Law Review

How do treaties change over time? This Article joins a growing body of scholarship focusing not on formal change mechanisms but instead on informal change arising from a treaty’s implementation in practice. Informal implementation is often murky, poorly documented, and may be indistinguishable from noncompliance. Yet it is significant both doctrinally under the Vienna Convention on the Law of Treaties—a set of rules for the formation and operation of treaties—and in its own right, when it does not meet the requirements to be doctrinally relevant. Based on a deep dive into the history of one of the oldest areas of …

New Host Records And Range Extensions For Helminth Parasites From Wading Birds In Southeastern Florida, Sarah Gumbleton, David Kerstetter, Amy Hirons, Christopher Blanar

Marine & Environmental Sciences Faculty Articles

Six species of wading birds collected from wildlife centers throughout South Florida were dissected for parasites. Twenty-six species of parasites represent new host records and five parasite species represent new geographic range extensions.

Indole-3-Glycerol Phosphate Synthase Ligand Binding Interactions, Oshane Thomas

Theses, Dissertations and Culminating Projects

Indole-3-glycerol phosphate synthase (IGPS) catalyzes the irreversible ring closure of 1-(ocarboxyphenylamino)- 1-deoxyribulose 5- phosphate (CdRP) into indole-3-glycerol phosphate (IGP) during the fourth step in tryptophan biosynthesis. Analysis of the crystal structure of IGPS from Mycobacterium tuberculosis (MtIGPS) hinted the importance of Lys119 for binding or catalysis. Lys110 from sulfolobus sulfataricus IGPS (ssIGPS) that aligns with Lys119 from MtIGPS, was proposed to be a general acid for the proton transfer that initiates the ring closure and decarboxylation of CdRP. To study the importance of Lys119 in the chemical mechanism for MtIGPS, an amino acid change was made at this position to …

Conocimiento, Actitudes Y Prácticas Sobre El Zika: Implicancias Programaticas Para La Prevencion Del Zika En Republica Dominicana, Breakthrough Research

Poverty, Gender, and Youth

This brief provides key insights for implementers of social and behavior change activities for Zika prevention in the Dominican Republic. The insights are based on data from a representative sample of people living in select USAID Zika program implementation communities and support an evidence-based approach for programming to increase preventative behaviors for Zika and other Aedes aegypti-transmitted viruses. The data indicate a need to narrow existing knowledge gaps related to the Zika virus, particularly its health consequences and effective preventive practices, and communicate more specifically about preventative behaviors to enable effective action.

Zika-Related Knowledge, Attitudes, And Practices: Programmatic Implications For Zika Prevention In Guatemala, Breakthrough Research

Poverty, Gender, and Youth

This brief provides key insights for implementers of social and behavior change (SBC) activities for Zika prevention in Guatemala. The insights are based on data from a representative sample of people living in select USAID Zika program implementation communities and support an evidence-based approach for programming to increase preventative behaviors for Zika and other Aedes aegypti-transmitted viruses. The data indicate a need to narrow existing knowledge gaps related to the Zika virus, particularly its health consequences and effective preventive practices, and communicate more specifically about preventative behaviors to enable effective action.

Zika-Related Knowledge, Attitudes, And Practices: Programmatic Implications For Zika Prevention In El Salvador, Breakthrough Research

Poverty, Gender, and Youth

This brief provides key insights for implementers of social and behavior change (SBC) activities for Zika prevention in El Salvador. The insights are based on data from a representative sample of people living in select USAID Zika program implementation communities and support an evidence-based approach for programming to increase preventative behaviors for Zika and other Aedes aegypti-transmitted viruses. The data indicate a need to narrow existing knowledge gaps related to the Zika virus, particularly its health consequences and effective preventive practices, and communicate more specifically about preventative behaviors to enable effective action.

Conocimiento, Actitudes Y Prácticas Sobre El Zika: Implicancias Programaticas Para La Prevencion Del Zika En El Salvador, Breakthrough Research

Poverty, Gender, and Youth

This brief provides key insights for implementers of social and behavior change activities for Zika prevention in El Salvador. The insights are based on data from a representative sample of people living in select USAID Zika program implementation communities and support an evidence-based approach for programming to increase preventative behaviors for Zika and other Aedes aegypti-transmitted viruses. The data indicate a need to narrow existing knowledge gaps related to the Zika virus, particularly its health consequences and effective preventive practices, and communicate more specifically about preventative behaviors to enable effective action.

Early Life Child Micronutrient Status, Maternal Reasoning, And A Nurturing Household Environment Have Persistent Influences On Child Cognitive Development At Age 5 Years: Results From Mal-Ed, Benjamin J J. Mccormick, Stephanie A. Richard, Laura E. Caulfield, Laura L. Pendergast, Jessica C. Seidman, Beena Koshy, Reeba Roshan, Rita Shrestha, Erling Svensen, Muneera Rasheed

Department of Paediatrics and Child Health

Background: Child cognitive development is influenced by early-life insults and protective factors. To what extent these factors have a long-term legacy on child development and hence fulfillment of cognitive potential is unknown.
Objective: The aim of this study was to examine the relation between early-life factors (birth to 2 y) and cognitive development at 5 y.
Methods: Observational follow-up visits were made of children at 5 y, previously enrolled in the community-based MAL-ED longitudinal cohort. The burden of enteropathogens, prevalence of illness, complementary diet intake, micronutrient status, and household and maternal factors from birth to 2 y were extensively measured …

Improved Sample Preparation Method For Lc-Ms/Ms Based Cellular Proteomics And Co-Immunoprecipitation Cross-Linking Proteomics Study Of Toll-Like Receptor 4, A.D.A Shahinuzzaman

Chemistry & Biochemistry Dissertations - Archive

Mass spectrometry-based methods are now widely practiced in large-scale proteomics studies. Unfortunately, sample loss in proteomics hinders complex analysis in proteomics, and immune precipitation (IP) mass spectrometry-based proteomics is getting popular in studying comprehensive signaling pathways. To contribute to these demanding research areas, I have completed two experimental projects in this thesis. In our first project, we developed a sample preparation method to identify proteins from detergent-containing cell lysate samples efficiently. Methanol-chloroform purification is an essential step in LC-MS/MS based cellular proteomics experiments since it removes salts and detergents from the sample. The cell lysis buffer contains a high concentration …

Investigation Of Microbiota In Health And Disease Of Poultry, Bishnu Adhikari

Graduate Theses and Dissertations

The microbiotas play vital roles in health and diseases of both humans and animals. 16S rRNA genes sequence analysis is one of the most popular and commonly used methods in the analysis of microbiotas associated with hosts. In this dissertation, the microbiotas of chickens (broilers, breeders, and layers) and turkeys were evaluated by 16S rRNA gene sequencing. Characterization of the culturable subpopulations of Lactobacillus in the chicken gut can serve as a valuable resource for probiotic development. In Chapter 2, Lactobacillus subpopulations recovered on MRS from chicken gut were defined comprehensively for the first time using 16S rRNA gene profiling, …

Selectivity Of Infrared Heat Treatment On Inactivation Of Mycotoxigenic Fungi On Stored Grain, Shantae A. Wilson

Graduate Theses and Dissertations

Selective Infrared (IR) heating holds great potential to decontaminate spores of unsafe fungi in corn. The objectives for this study were to investigate the impact of exposing corn to infrared energy at selected peak wavelengths (λ), infrared intensities and treatment durations, followed by tempering for further inactivation of microbes on the grain and explore a method for decontaminating Aspergillus flavus (A. flavus) spores on corn. Freshly harvested corn with initial moisture contents (IMCs) of 16%, 20%, and 24% wet basis (w.b.) were used. The corn samples were treated at different infrared wavelengths (3.2, 4.5, and 5.8 μm) for 20, 40 …

Barriers Of African American Football Student-Athletes In Seeking Mental Health Services, Todd Andrew Wilkerson

Graduate Theses and Dissertations

Nearly half (48%) of collegiate football student-athletes are African American (NCAA, 2018). African American student-athletes face adversity at their respective institutions in the forms of racism and unfair treatment (Hill, Hall & Appleton, 2010). African American male student-athletes face educational stressors, campus stressors and athletic stressors. These stressors consist of academics, family, athletics and social relationships (Miller & Hoffman, 2009). Many African American student-athletes do not seek mental health treatment due to their status on campus (Watson, 2006). However, few studies have examined mental health and barriers for African American male student-athletes when seeking mental health services. As such, the …