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2018

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Fracture Resistance Of Endodontically Treated Premolars With Deep Cervical Lesion Restored With And Without Post In Different Restorations, Phrohphrim Suebsawadphatthana Jan 2018

Fracture Resistance Of Endodontically Treated Premolars With Deep Cervical Lesion Restored With And Without Post In Different Restorations, Phrohphrim Suebsawadphatthana

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: The purpose of this study was to evaluate the effects of fiber posts and different restorations on the fracture resistance of endodontically treated maxillary premolars with cervical cavities. Methods: In forty extracted human first maxillary premolars, cervical cavities were prepared simulating cervical lesions and randomly divided into four groups (n=10): COM-NP, endodontically treated teeth (ETT) restored with resin composite without fiber posts; ON-NP, ETT restored with CAD/CAM ceramic onlays without fiber posts; COM-P, ETT restored with resin composite and fiber posts; and CR-P, ETT restored with CAD/CAM ceramic crowns and fiber posts. After the fiber posts and restorations had …

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Effect Of Bitter Gourd Extracts On The Mutagenicity Of 1-Aminopyrene Reacted With Nitrite Using Ames Test, Korkiat Panpasuk Jan 2018

Effect Of Bitter Gourd Extracts On The Mutagenicity Of 1-Aminopyrene Reacted With Nitrite Using Ames Test, Korkiat Panpasuk

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study was conducted to investigate the mutagenic activity of raw and cooked bitter gourd (Momordica charantia) including raw fruit extract (RW), blanching water (BW), hot water extract from blanched fruit (HB), stir-fried fruit extract (SF), and stir-fried fruit (pre-squeezed with salt) extract (SS). In addition, the effects of extracts in modifying the mutagenic activity of mutagen freshly prepared by the reaction between 1-aminopyrene and sodium nitrite and the effects when adding each extract at the beginning of the same reaction using Ames test on Salmonella typhimurium strains TA98 and TA100 without enzymatic activation were also determined. The data for …

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Antioxidant And Antimutagenic Activity Of Extracts From Pericarp And Seed Of Zanthoxylum Limonella Alston, Patamawan Wipoosanapan Jan 2018

Antioxidant And Antimutagenic Activity Of Extracts From Pericarp And Seed Of Zanthoxylum Limonella Alston, Patamawan Wipoosanapan

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study was conducted to investigate the antioxidant and antimutagenic activity of Zanthoxylum limonella seed extract and that of Z. limonella pericarp extract. The total phenolic contents and antioxidant activity assay including DPPH assay and FRAP assay were performed. The results showed that the antioxidant activity of pericarp extract was higher than that of the seed extract. The direct mutagenicity assay was determined, with and without nitrite treatment using the Ames test towards Salmonella typhimurium strains TA98 and TA100. The mutagenic modification assay was performed by measuring the interfering effect of the pericarp extract and the seed extract on the …

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Seismic Characteristics Of Paleo-Pockmarks At Great South Basin, New Zealand, Arunee Karaket Jan 2018

Seismic Characteristics Of Paleo-Pockmarks At Great South Basin, New Zealand, Arunee Karaket

Chulalongkorn University Theses and Dissertations (Chula ETD)

Paleo-pockmarks and present-day pockmarks are founded in several varieties both onshore and offshore worldwide. These features can be used as an indicator of fluid flow process which expulses through an unconsolidated sediment within the basin. The Great South Basin, New Zealand is one of the basins that paleo-pockmarks are observed covering the southeast offshore of South Island at around 1.5 s (TWT) depth beneath the surface. This study aims to identify seismic characteristics of paleo-pockmarks in the Great South Basin and predict the possible fluid origins and their mechanism. The numerous paleo-pockmarks are identified and imaged by using 3D seismic …

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Utilization Of Biochar Coupled With Coal Combustion Products For Degraded Soil Amendment, Thidphavanh Sengsingkham Jan 2018

Utilization Of Biochar Coupled With Coal Combustion Products For Degraded Soil Amendment, Thidphavanh Sengsingkham

Chulalongkorn University Theses and Dissertations (Chula ETD)

Low Salinity Waterflooding (LSWF) has been intensively studied worldwide as it is a low-cost technique. By injecting water with very low salinity compared to formation water in the reservoir, the method changes equilibrium between rock surface, oil and surrounding water, causing wettability alteration through Multi-component Ion Exchange (MIE) mechanism. In this study, ammonium ion is added into low salinity water to facilitate the MIE mechanism in shaly-sandstones reservoir. Experiments are performed with shaly-sandstone rock as it contains both calcium and magnesium ions as part of clays and ammonium ion can express its potential by triggering MIE mechanism. Stirring test, spontaneous …

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Crude Oil Removal By Sphingobium Sp. Mo2-4 And Bacillus Megaterium Tl01-2 Immobilized On Aquaporous Gel, Adisan Rungsihiranrut Jan 2018

Crude Oil Removal By Sphingobium Sp. Mo2-4 And Bacillus Megaterium Tl01-2 Immobilized On Aquaporous Gel, Adisan Rungsihiranrut

Chulalongkorn University Theses and Dissertations (Chula ETD)

Petroleum products are the major energy sources that drive global economy. The exploration and exploitation of crude petroleum oil increase the potential risk for oil spills in marine ecosystem. In this work, two non-pathogenic sponge-associated bacteria were developed as immobilized bacterial co-culture for crude oil removal from seawater. Two bacteria were Spingobium sp. MO2-4 that could degrade crude oil, diesel oil, and fuel oil, and Bacillus megaterium TL01-2 that could degrade crude oil, possessed high cell surface hydrophobicity, could tolerate heavy metal, and was able to form biofilm.. The modified polyurethane foam, aquaporous gel (AQ), was used as carrier for …

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Diesel Removal Efficiency In Soil Washing Process By Mixed Solution Of Nonionic Surfactant And Biosurfactant, Pajaree Boonyathai Jan 2018

Diesel Removal Efficiency In Soil Washing Process By Mixed Solution Of Nonionic Surfactant And Biosurfactant, Pajaree Boonyathai

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this study, Tween 80 and three biosurfactant (Rhamnolipid, Saponin and Lipopeptide) were mixed to formulate the appropriate surfactant solution for diesel removal from contaminated soil by washing process. The mixed surfactant system was fixed at 5% w/v of active surfactants concentration in total with different mass ratio of Tween 80 to biosurfactant at 9:1, 8:2 and 7:3 of total concentration. The critical micelle concentration (CMC) of various ratio surfactant mixtures and their interfacial tension (IFT) with diesel were determined and compared with their individual surfactant. The results illustrate that mixed Tween and Lipopeptide at 9:1 mass ratio had the …

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Formulation Of Oil Dispersants By Mixing Lipopeptide Biosurfactant And Non-Ionic Surfactants, Parisarin Nawavimarn Jan 2018

Formulation Of Oil Dispersants By Mixing Lipopeptide Biosurfactant And Non-Ionic Surfactants, Parisarin Nawavimarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Marine oil spill can cause various harmful impacts to the environment. To solve this problem, dispersant is usually applied to enhance petroleum remediation in seawater. The commercial dispersants mostly contain surfactant and solvent that can be toxic to marine lives. This study aimed to formulate solvent-free biosurfactant-based dispersants by mixing lipopeptides from Bacillus subtilis GY19 with fatty alcohol ethoxylate (Dehydol LS7TH), a low toxicity nonionic surfactant. Hydrophilic-lipophilic deviation (HLD) concept was applied. The fractions of lipopeptide and dehydol LS7TH were determined based on the equivalent alkane carbon number (EACN) of hydrocarbons under seawater salinity (34ppt). The molar fractions of lipoppeptide …

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Pm10 And Pm2.5 Concentrations In Bangkok Over 10 Years And Implications For Air Quality, Tin Thongthammachart Jan 2018

Pm10 And Pm2.5 Concentrations In Bangkok Over 10 Years And Implications For Air Quality, Tin Thongthammachart

Chulalongkorn University Theses and Dissertations (Chula ETD)

Atmospheric particulate matter particles with a diameter less than 10 micron (PM10) and less than 2.5 micron (PM2.5) have been adverse human health effect. Ambient air pollution data in Central Business District (CBD) of Bangkok monitored by Pollution Control Department and Bangkok Metropolitan Administration were collected over ten years in Bangkok from 2008 to 2017. Trend of the particulate matter concentrations data were investigated over ten year for implications of activities related to air pollution. Daily average air pollution data between year 2015 and year 2017 were used as the dependent variables to develop mathematic models with multiple linear regression …

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System Dynamics Analysis Of Sugar-Sweetened Beverage Taxing Effect On Sugar Consumption, Dental Health Service Utilization And Oral Health For Thai Adults And Elderly, Nipaporn Urwannachotima Jan 2018

System Dynamics Analysis Of Sugar-Sweetened Beverage Taxing Effect On Sugar Consumption, Dental Health Service Utilization And Oral Health For Thai Adults And Elderly, Nipaporn Urwannachotima

Chulalongkorn University Theses and Dissertations (Chula ETD)

Dental caries is a major oral health problem, particularly among developing countries. Almost half of Thai adults and elderly have at least one untreated dental caries. Dental caries can result in debilitating pain that affects work productivity and even the performance of daily activities. The objectives of this study are to develop system dynamics model addressing the relationship of dental caries, sugar consumption and dental health service utilization of Thai population aged 15 and older; and to estimate the changes of dental caries prevalence when the sugar-sweetened beverage tax is implemented. A multi-sector system dynamics model was developed to consolidate …

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Forecasting International Tourist Arrivals From Major Countries To Thailand, Ontheera Hwandee Jan 2018

Forecasting International Tourist Arrivals From Major Countries To Thailand, Ontheera Hwandee

Chulalongkorn University Theses and Dissertations (Chula ETD)

Tourism industry is one of industries that are very important for Thai economy. In order to achieve effective marketing and resource planning, accurate forecasting of tourist arrivals from major countries to Thailand is necessary for Thai tourism industry. In this paper, various forecasting models are explored to forecast monthly tourist arrivals from China, Malaysia, Korea, Japan, Russia, UK and US. The proposed models include both time series models, i.e., SARIMA, Holt-Winter, and explanatory models, i.e., Multiple Regression and Feed Forward Artificial Neural Networks (FANNs). Economic factors such as income, relative price, exchange rates, and dummy variables of seasonality and news …

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Chin Diaspora And Their Social Networks In Thailand : Implications For Development In Myanmar, Pau Sian Lian Jan 2018

Chin Diaspora And Their Social Networks In Thailand : Implications For Development In Myanmar, Pau Sian Lian

Chulalongkorn University Theses and Dissertations (Chula ETD)

Due to socioeconomic and political reasons, Chin ethnic people from the western part of Myanmar have been migrating to inner part of the country and abroad. Economic growth and requirement of low skill labor in Thailand since the 1980s have been the pull factors for low skill migrants from its neighbors, including Myanmar. Chin people came to Thailand not only for economic opportunities but also for onward migration. The purpose of this research is to explore the social networks of Chin diaspora in Thailand and their implication to the economic and political development of their home community in Myanmar. With …

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Effect Of Temperature On Antioxidant Enzyme Activities And Gene Expression In Sand Worm Perinereis Quatrefagesi (Grube, 1878), Sucharat Suksai Jan 2018

Effect Of Temperature On Antioxidant Enzyme Activities And Gene Expression In Sand Worm Perinereis Quatrefagesi (Grube, 1878), Sucharat Suksai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Polychaetes is one of intertidal animals that face the fluctuations of coastal environment through time. One of important environmental factors to animals is temperature regarding to the fluctuation of temperature in intertidal environment. Human activities could also cause high changes in water temperature by high temperature discharges from urban and industries. Since global warming is still a main issue, an increase of seawater temperature about 3°C could be possible as a prediction. This study aimed to investigate effect of temperature in polychaetes Perinereis quatrefagesi at cellular level, which could be indicated by heat shock response and oxidative stress due to …

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The Measurement Of Transverse Relaxation Time (T2) Using Histogram From Roi Setting In Muscle Activity Study At 1.5 Tesla Mri, Kannikar Kanyakham Jan 2018

The Measurement Of Transverse Relaxation Time (T2) Using Histogram From Roi Setting In Muscle Activity Study At 1.5 Tesla Mri, Kannikar Kanyakham

Chulalongkorn University Theses and Dissertations (Chula ETD)

Muscle functional MRI (mfMRI) is the method to evaluate the muscle activity before and immediately after the muscle exercise by the magnetic resonance images. Exercise of skeletal muscles enhances image contrast in T2-weighted magnetic resonance (MR) images. In previous studies, the exercised physiology used multiple-spin-echo (MSE) sequences for calculating transverse relaxation time (T2) with several small regions of interest (ROI) within the target tissue. Such a method may not represent all properties of the target tissue. The purpose of this study is to measure T2 in rest and exercise muscle at 1.5 Tesla MRI using average mode values in histogram …

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The Pharmacokinetic Model Of 18f-Fdopa In Pet Brain Imaging For Early Parkinson’S Disease, Wirunpatch Buratachwatanasiri Jan 2018

The Pharmacokinetic Model Of 18f-Fdopa In Pet Brain Imaging For Early Parkinson’S Disease, Wirunpatch Buratachwatanasiri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Parkinson’s disease (PD) symptom usually appears when over half of all dopaminergic neurons have died. Early detection and treatment approach are; therefore, very important. The 18F-FDOPA PET scan is extensively examined to differentiate the normal and pathological dopamine metabolism in the human brain. The purpose of this study was to investigate the transfer rate constants of 18F-FDOPA in PET brain imaging based on compartmental model in early Parkinson’s disease. The retrospective data from five early PD patients who underwent 18F-FDOPA PET brain scan at King Chulalongkorn Memorial Hospital (KCMH) were collected. After 18F-FDOPA was administered intravenously, PET images were acquired …

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Characterization Of Leptospiral Extracellular Vesicles In Stress Conditions, Eakalak Phanchamnan Jan 2018

Characterization Of Leptospiral Extracellular Vesicles In Stress Conditions, Eakalak Phanchamnan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Pathogenic Leptospira spp. is a causative agent of leptospirosis, a worldwide zoonosis with public health concern especially in the urban slum of metropolis and rural areas in tropical and subtropical countries. The pathogenesis of leptospirosis remains elusive. Extracellular vesicles (ECVs), which pinch off from the bacterial membranes, simultaneously harbor multiple active molecules that may serve as a secretion system, communication tool, and vaccine candidates. Recently, chemically induced leptospiral ECVs were studied and used as vaccine candidates. However, the naturally produced leptospiral ECVs has not been characterized. This study aimed to identify proteins in leptospiral ECVs produced under stress conditions including …

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The Role Of Histone Methylation Of H3k4 In Polarization Of Macrophages Stimulated By Immune Complex And Lipopolysaccharide And Its Therapeutic Potential In Sepsis, Vichaya Ruenjaiman Jan 2018

The Role Of Histone Methylation Of H3k4 In Polarization Of Macrophages Stimulated By Immune Complex And Lipopolysaccharide And Its Therapeutic Potential In Sepsis, Vichaya Ruenjaiman

Chulalongkorn University Theses and Dissertations (Chula ETD)

Macrophage plasticity is a process that allows macrophages to switch between two opposing phenotypes based on differential stimuli. Functional plasticity of macrophages contributes to disease pathogenesis such as cancer, metabolic diseases, autoimmune diseases and systemic infections. Epigenetics plays an important role in regulating this process. Macrophages stimulated with lipopolysaccharide (LPS) (M(LPS)) produce high level of pro-inflammatory cytokines such as IL-12, TNFα and IL-6 and low level of anti-inflammatory cytokine IL-10, while macrophages stimulated with LPS in the presence of immune complex (IC) (M(IC)) produce high level of IL-10 and low level of IL-12. In this study, we investigated the plasticity …

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Comprehensive Proteomics Identification Of Ifn-Λ3-Regulated Antiviral Proteins In Hbv-Transfected Cells, Jiradej Makjaroen Jan 2018

Comprehensive Proteomics Identification Of Ifn-Λ3-Regulated Antiviral Proteins In Hbv-Transfected Cells, Jiradej Makjaroen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Interferon (IFN)-λ is a relatively unexplored, yet promising anti-viral agent. IFN-λ has recently been tested in clinical trials of chronic hepatitis B, with the advantage that side effects may be limited compared with IFN-α, as IFN-λ receptors are found only in epithelial cells. To date, IFN-λ downstream signaling pathway remains largely unelucidated, particularly via proteomics methods. Here, we report that IFN-λ3 inhibits HBV replication in HepG2.2.15 cells, reducing levels of both HBV transcripts and intracellular HBV DNA. Quantitative proteomic analysis of HBV-transfected cells was performed following 24-hour IFN-λ3 treatment, with parallel IFN-α2a and PBS treatments for comparison using a dimethyl …

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The Severity Of Cryptococcus Neoformans Infection In Fc Gamma Receptor Iib Deficient Mice, Saowapha Surawut Jan 2018

The Severity Of Cryptococcus Neoformans Infection In Fc Gamma Receptor Iib Deficient Mice, Saowapha Surawut

Chulalongkorn University Theses and Dissertations (Chula ETD)

Cryptococcus neoformans, an encapsulated yeast commonly infecting the central nervous system, refers to as cryptococcal meningitis or cryptococcosis is commonly found in Thailand. C. neoformans is able to replicate, survive inside host macrophages, evade from the cells and use macrophages as a vehicle to disseminate to the target organs, a mechanism known as Trojan horse. Therefore, the containment of C. neoformans in phagocytic cell is important for host protective immunity. Cryptococcosis is common in immunocompromised host such as in patients with low CD4+ T helper cells in HIV-infection and patients taking immunosuppressive drugs. However, the incidence of cryptococcosis in healthy …

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Hsv-1 Induces Filopodia Formation In Activated T Lymphocytes, Thanayod Sasivimolrattana Jan 2018

Hsv-1 Induces Filopodia Formation In Activated T Lymphocytes, Thanayod Sasivimolrattana

Chulalongkorn University Theses and Dissertations (Chula ETD)

Herpes simplex virus type 1 (HSV-1) is a widespread pathogen, which has diverse clinical manifestations from mild localized infection to severe systemic infection. The tissue tropism of HSV-1 is mainly epithelial and mucosal cells. However, it can also multiply in T lymphocytes suggesting a cause of viremia. In this study, CD3+CD4-CD8- Jurkat T lymphocytes were used. Under activation, up-regulation of CD69 molecules on cell surface was observed. Increase yield of HSV-1 production in activated T lymphocytes was demonstrated. In addition, most of HSV-1 virion progenies from infected T lymphocytes were released extracellularly, suggesting the cause of dissemination. The mechanism which …

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Phosphoproteomic Analysis Of Fc Gamma Receptor Iib Deficient Macrophages In Enhancing The Severity Of Endotoxin Tolerance, Thunnicha Ondee Jan 2018

Phosphoproteomic Analysis Of Fc Gamma Receptor Iib Deficient Macrophages In Enhancing The Severity Of Endotoxin Tolerance, Thunnicha Ondee

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sepsis is a severe response to systemic infection and one of the leading causes of death in patients world-wide. Bacterial endotoxin (lipopolysaccharide; LPS), is a potent inducer of inflammation that has been associated with the pathophysiology of sepsis. The repeated LPS exposure can induce a suppressive effect as refer to endotoxin tolerance. Fc gamma receptor IIb (CD32b), the only inhibitory receptor among FcGR family, associates with systemic lupus erythematosus (FcGRIIb defunctioning polymorphisms) which are commonly found in Asian populations. Because of an inhibitory nature of the signaling, endotoxin tolerance in lupus with FcGRIIb deficient (FcGRIIb-/-) is interesting and might be …

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Host And Viral Factors Influencing Disease Progression And Treatment Response In Chronic Hbv Infection, Umaporn Limothai Jan 2018

Host And Viral Factors Influencing Disease Progression And Treatment Response In Chronic Hbv Infection, Umaporn Limothai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hepatitis B virus (HBV) infection represents a major risk factor of developing chronic hepatitis, cirrhosis and hepatocellular carcinoma (HCC). The clinical outcome of HBV infection depends on complex of host and virus interactions. In this study, we found that serum HBV RNA levels has emerged as a potential viral marker for active HBV infection and may be used to evaluate response to antiviral therapy and disease progression. Serum HBV RNA levels varied among different phases of chronic HBV infection (CHB). There was a correlation between serum HBV RNA levels and amount of intrahepatic covalently closed circular DNA (cccDNA), suggesting that …

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The Difference Patterns Of Electrophysiological Properties And Effects Of Kynurenic Acid In Trigeminal Ganglion Neurons Isolated From Rats Migraine Models, Naeemah Haji-A-Sa Jan 2018

The Difference Patterns Of Electrophysiological Properties And Effects Of Kynurenic Acid In Trigeminal Ganglion Neurons Isolated From Rats Migraine Models, Naeemah Haji-A-Sa

Chulalongkorn University Theses and Dissertations (Chula ETD)

Migraine headache generates in trigeminal nociceptive system which is activated by cortical spreading depression (CSD) phenomenon. There are also some neurotransmitter changes relating to pathogenesis of migraine, one of representative examples is serotonin (5-HT) depletion that may predispose to migraine development. Trigeminal ganglion (TG) is a structure containing cell body of neurons that undertakes a role as first order neuron in trigeminal nociceptive system. Moreover, the alteration of glutamate may contribute to hyperactivity of its specific receptors, for instance N-methyl-D-aspartate (NMDA) receptor. Endogenous biomolecule, such as kynurenic acid (KYNA) which is derived form of 5-HT can inhibit NMDA receptor. Basically, …

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Anticancer Activity Of Plumbagin On Stem-Like Characteristics, Tumor Angiogenesis And Metastatic Potential In Endocrine Resistant Breast Cancer, Nithidol Sakunrangsit Jan 2018

Anticancer Activity Of Plumbagin On Stem-Like Characteristics, Tumor Angiogenesis And Metastatic Potential In Endocrine Resistant Breast Cancer, Nithidol Sakunrangsit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Plumbagin (PLB), a naphthoquinone compound and vitamin K3 derivative, was shown its potent cytotoxicity and anti-invasion in anti-hormonal resistant cells through the inhibition of Snail-induced epithelial mesenchymal transition (EMT). Overexpression of Snail leads to decrease E-cadherin and increase of beta-catenin, resulting in the activation of Wnt pathway that increases cancer stem-like characteristics in these resistant cells. This study was aimed to investigate the inhibitory effects of PLB on cancer stem-like cells (CSLCs), angiogenesis and Wnt signaling-mediated cell proliferation and invasion. In addition, our study also focused on the anticancer activity of PLB in anti-hormonal resistant breast cancer in vivo. Both …

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Metabolic Engineering Of Synechococcus Elongatus Pcc 7942 For Mycosporine-2-Glycine Production Under Salt Stress Condition, Panwad Pingkhanont Jan 2018

Metabolic Engineering Of Synechococcus Elongatus Pcc 7942 For Mycosporine-2-Glycine Production Under Salt Stress Condition, Panwad Pingkhanont

Chulalongkorn University Theses and Dissertations (Chula ETD)

ไมโครสปอรีน-ไลก์ อะมิโน แอซิด (MAAs) เป็นกลุ่มของสารเมแทบอไลต์ทุติยภูมิ สังเคราะห์โดยจุลินทรีย์และสาหร่ายหลายชนิด สารกลุ่มนี้มีหน้าที่สำคัญในการคัดกรองรังสียูวี และยังมีหน้าที่อื่นๆ ได้แก่ การต้านอนุมูลอิสระ ปกป้องเซลล์จากภาวะเครียด และควบคุมการออสโมซิส โดยไมโครสปอรีน-2-ไกลซีน (M2G) เป็นหนึ่งในสารกลุ่ม MAAs ที่มีความสามารถในการต้านอนุมูลอิสระมากกว่าสารอื่นๆ ในกลุ่มเดียวกัน ดังนั้นจึงเป็นที่สนใจที่จะเพิ่มการผลิต M2G เพื่อนำไปประยุกต์ใช้ในอุตสาหกรรมความงาม และเวชสำอาง ในการศึกษานี้ประสบความสำเร็จในการแสดงออกของยีนในชีวสังเคราะห์ M2G (Ap3858-3855) จาก Aphanothece halophytica ในไซยาโนแบคทีเรียน้ำจืด Synechococcus elongatus PCC 7942 จากการวิเคราะห์การถอดรหัสของยีน Ap3858-3855 พบว่า ยีนทั้ง 4 ยีนนี้เป็น monocistronic mRNA heterologous expression ของยีน Ap3858-3855 ใน S. elongatus พบว่าทุกยีนมีการแสดงออกมากขึ้นภาวะเครียดจากเกลือ NaCl ยีน Ap3857 แสดงออกเพิ่มมากที่สุด 2.39 ± 0.25 เท่า และภายใต้ภาวะเครียดจากเกลือ KCl ยีน Ap3856 แสดงออกเพิ่มมากที่สุด 3.70 ± 0.35 เท่า ระดับการแสดงออกของยีนที่เกี่ยวข้องกับภาวะเครียดออกซิเดชั่น (sodB, cat และ tpxA) ในเซลล์แสดงออก พบว่าภายใต้ภาวะเครียดจากเกลือ NaCl มีเพียงยีน tpxA แสดงออกมากขึ้น 4.58 ± 0.58 เท่า ขณะที่ภาวะเครียดจากเกลือ KCl กระตุ้นการแสดงออกของ sodB และ tpxA 4.09 ± 0.86 และ 11.81 ± 1.02 เท่า ตามลำดับ ส่วนการแสดงออกของยีนที่เกี่ยวข้องกับสารออสโมไลท์ ได้แก่ Synpcc7942_0808 และ …

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Preparation Of Graphene Nanocomposites For Anticorrosion And Sensors, Nadtinan Promphet Jan 2018

Preparation Of Graphene Nanocomposites For Anticorrosion And Sensors, Nadtinan Promphet

Chulalongkorn University Theses and Dissertations (Chula ETD)

This dissertation focused on the development of graphene (GO) nanocomposite coating to improve various substrate properties for the sensor application, which is divided into 2 main parts. The first part is the development of GO incorporated into electroless nickel phosphorus (NiP) coating to improve the properties of metal for electrochemical sensor application. Nickel phosphorus - titanium dioxide sol - reduced graphene oxide (NiP-TiO2 sol -RGO) coated surface was successfully prepared by one-pot electroless deposition on the steel substrate. The results show that the homogeneously formation of water-repellent RGO film covering on the coated surface leading to improve the corrosion resistance …

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Synthesis Of High Efficiency Amidoxime Polymer Adsorbents For Uranium Extraction From Seawater By Radiation-Induced Polymerization, Wijittra Wongjaikham Jan 2018

Synthesis Of High Efficiency Amidoxime Polymer Adsorbents For Uranium Extraction From Seawater By Radiation-Induced Polymerization, Wijittra Wongjaikham

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study synthesized amidoxime polymer gel for uranium extraction from seawater through a polymerization process with gamma ray or UV-C radiation. The synthesis started with a mixture of acrylonitrile (AN) and methacrylic acid (MAA) monomers together with methylene bisacrylamide as a crosslinker and hydrogen peroxide in the case of UV radiation. The mixture was irradiated with gamma ray at various doses or UV-C for various durations. The resulting polymer gel was ground into fine particles and immersed in a hydroxylamine hydrochloride solution for 90 minutes in order to convert the chemical functional group from the cyano group into the amidoxime …

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Struggling To Live A New Normal Life Among Chinese Women After Losing The Only Child, Haiyan Wang Jan 2018

Struggling To Live A New Normal Life Among Chinese Women After Losing The Only Child, Haiyan Wang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Losing the only child was a destructive disaster that had destroyed Chinese women's life and health in all dimensions. However, there is no suitable theory or knowledge to guide nursing practice to these women. This study, thus, employed Glaserian grounded theory to discover substantive theory on the living process of Chinese women after losing the only child. Purposive sampling, snowball sampling, and theoretical sampling were used to recruited participants. Thirteen Chinese women who have lost the only child aged 50 to 68 years old recruited. Data were collected through in-depth interview, observation, and field notes. Data were analyzed by the …

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Assessment Of Attitudes And Knowledge Of General Dental Practitioners In Providing Implant Maintenance, Aimwalee Rudeejaraswan Jan 2018

Assessment Of Attitudes And Knowledge Of General Dental Practitioners In Providing Implant Maintenance, Aimwalee Rudeejaraswan

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to survey the attitudes and knowledge of Thai general dental practitioners (GDPs) in proving dental implant maintenance care. The online questionnaire was sent to Thai GDPs who graduated between 2000 and 2016. Of the returned 435 questionnaires, 429 completely filled were included for analysis. 76% of the participants were female and 24% were male. Half of the participants were aged 28-33 years old and 78% of the participants have been working as a GDP 10 years or below. There were 64.3% and 59.4% of the participants who agreed that dental implant surgery and restoration should only be …

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Comparison Of The Implant Deviation Between Implants Placed Using Static And Dynamic Computer Assisted Surgery Methods, Dechawat Kaewsiri Jan 2018

Comparison Of The Implant Deviation Between Implants Placed Using Static And Dynamic Computer Assisted Surgery Methods, Dechawat Kaewsiri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: The aim of this study was to compare the accuracy of implant placement in terms of deviations related to the virtual plan between static and dynamic computer assisted implant surgery (CAIS) systems in single tooth space. Materials & Methods: 60 single implants were randomly placed using two difference CAIS systems by one surgeon. Preoperative CBCT transferred to implant planning software were used to plan the virtual implant position. Implants were placed using either stereolithographic guide template for static CAIS group (n = 30) or implant navigation system for dynamic CAIS group (n = 30). Postoperative CBCT were taken to …

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