Digital Commons Network^™

Nktr-214 (Cd122-Biased Agonist) And Nktr-262 (Tlr7/8 Agonist) Combination Treatment Pairs Local Innate Immune Activation With Systemic Cd8+ T Cell Expansion To Enhance Anti-Tumor Immunity, Annah S. Rolig, Daniel Rose, Saul Kivimäe, Deborah Charych, Werner Rubas, Jonathan Zalevsky, William L. Redmond

Society for Immunotherapy of Cancer 2018 Annual Meeting Posters

Background: Radiation therapy (RT) remains the standard of care for many human cancers. Combining NKTR-214, a CD122-biased cytokine agonist conjugated with releasable polyethylene-glycol (PEG) chains, with local RT significantly enhanced therapeutic efficacy in preclinical models. Mechanistically, NKTR-214 provides sustained signaling through the IL-2 receptor pathway (IL-2Rβγ) to preferentially activate and expand effector CD8+ T and NK cells and RT modulates the tumor microenvironment (TME) to induce antigen-release. Together, NKTR-214/RT treatment resulted in improved therapeutic responses compared to either treatment alone. However, abscopal responses in murine tumors were modest, leading us to explore alternative approaches with the potential to elicit more …

Managing Lake Fertility Within The Guidelines Of A Nutrient Management Plan And Based On Algal Nutrient Limitation, Bradley J. Austin, J. Thad Scott, Brian E. Haggard

Arkansas Water Resources Center Technical Reports

The specific objectives were to first, monitor nutrients, algal biomass, and water clarity in lakes Cove, Spring, and Wedington. Second, evaluate whether algal growth in each of the lakes was limited by N, P, or both N and P. This research was conducted to help USFS better manage lake fertilization to maximize algal growth and improve the fisheries within these lakes.

Faculty Senate Meeting Minutes – November 2018, Faculty Senators

Minutes 2018-2019

This is the archived record of the November 14, 2018 faculty senate meeting minutes. Contact the Faculty Senate president or JagWorks Repository Coordinator to request access to this document.

Application Of A Light Switchable System In Redirecting Escherichia Coli Metabolic Fluxes, Xintong Xia

Graduate Theses - Chemical Engineering

Optogenetics has gained increasing attention for enabling reversible and non-invasive control in biochemical engineering. The purpose of this project is to investigate the application of a genetically engineered light-switchable system, Cph8-OmpR, for controlling the accumulation of the useful central metabolite glucose-6-phosphate (G6P) in Escherichia coli. This could be accomplished by linking expression of an adaptor protein, SspB, which increases enzyme phosphofructokinase (Pfk) degradation, with different light intensities. Pfk is the enzyme that catalyzes the conversion of fructose-6-phosphate (F6P), an isomer of G6P from an equilibrium reaction, to fructose 1,6-biphosphate for downstream metabolism. This step is considered the major control point …

Vol.46 N.15 November 1st 2018, Voice Media Ventures

Black Voice News

No abstract provided.

Women's Tennis Media Guide 2018-19, Wofford College. Department Of Athletics

Media Guides

No abstract provided.

Characterization And Effect Of Metal Ions On The Formation Of The Thermus Thermophilus Sco Mixed Disulfide Intermediate, Liezelle C. Lopez, Nikita Mukhitov, Lindsey D. Handley, Cristina S. Hamme, Cristina R. Hofman, Lindsay Euers, Jennifer R. Mckinney, Amani D. Piers, Ellen Wadler, Laura M. Hunsicker-Wang

Chemistry Faculty Research

The Sco protein from Thermus thermophilus has previously been shown to perform a disulfide bond reduction in the Cu_A protein from T. thermophilus, which is a soluble protein engineered from subunit II of cytochrome ba ₃ oxidase that lacks the transmembrane helix. The native cysteines on TtSco and TtCu_A were mutated to serine residues to probe the reactivities of the individual cysteines. Conjugation of TNB to the remaining cysteine in TtCu_A and subsequent release upon incubation with the complementary TtSco protein demonstrated the formation of the mixed disulfide intermediate. The cysteine of …

Trex1 Is Expressed By Microglia In Normal Human Brain And Increases In Regions Affected By Ischemia, Parul H. Kothari, Grant R. Kolar, Joanna C. Jen, Rula Hajj-Ali, Paula Bertram, Robert E. Schmidt, John P. Atkinson

Biological Sciences Faculty Research & Creative Works

Background: Mutations in the three-prime repair exonuclease 1 (TREX1) gene have been associated with neurological diseases, including Retinal Vasculopathy with Cerebral Leukoencephalopathy (RVCL). However, the endogenous expression of TREX1 in human brain has not been studied. Methods: We produced a rabbit polyclonal antibody (pAb) to TREX1 to characterize TREX1 by Western blotting (WB) of cell lysates from normal controls and subjects carrying an RVCL frame-shift mutation. Dual staining was performed to determine cell types expressing TREX1 in human brain tissue. TREX1 distribution in human brain was further evaluated by immunohistochemical analyses of formalin-fixed, paraffin-embedded samples from normal controls and patients …

The Setonian, November 1, 2018, Seton Hall University

2018

No abstract provided.

No. 16: Cheap Industrial Food And The Urban Margins, Tony Weis, Marylynn Steckley, Bruce Frayne

Hungry Cities Partnership

From the middle of the 20th century onwards, the productivity gains associated with high-input, high-yield monocultures and livestock operations have become increasingly central to global food security and to dynamics of urbanization across the global south. On one hand, competition has deflated prices and helped undermine the viability of small farm livelihoods in many places. On the other hand, rising flows of cheap food have effectively subsidized urban migration in impoverished urban and peri-urban settings. But this cheapness is highly deceptive, as it hinges on the failure to account for an array of biological and physical costs – which can …

Tumor Infiltrating Lymphocyte Recruitment After Peri-Lymphatic Irx-2 Cytokine Immunotherapy In Resectable Breast Cancer And Head And Neck Carcinoma, Joanna Pucilowska, Venkatesh Rajamanickam, Nikki Moxon, Monil Shah, Maritza Martel, Alison Conlin, James E. Egan, David B. Page

Society for Immunotherapy of Cancer 2018 Annual Meeting Posters

Background: The IRX-2 biologic is a subcutaneous injectable immunotherapy composed of IL-2 and other cytokines derived from stimulated lymphocytes. Preclinically, IRX-2 activates T cells, natural killer cells, macrophages, and dendritic cells, and facilitates maturation of antigen-presenting cells.Tumor-infiltrating lymphocytes (TILs) are associated with improved outcomes in many cancers including early stage breast cancer (ESBC) and head and neck squamous cell carcinoma (HNSCC). We report data on TIL recruitment associated with pre-operative IRX-2 in a phase Ib ESBC trial, as well as phase Ib and IIa HNSCC trials.

Methods: The pre-operative IRX-2 regimen was evaluated in both ESBC and HNSCC trials for …

Reactive Ion Plasma Modification Of Poly(Vinyl‐Alcohol) Increases Primary Endothelial Cell Affinity And Reduces Thrombogenicity, Patrick Jurney, Deirdre Anderson, Grace Pohan, Evelyn Yim, Monica Hinds

Faculty Publications

Bulk material properties and luminal surface interaction with blood determine the clinical viability of vascular grafts, and reducing intimal hyperplasia is necessary to improve their long‐term patency. Here, the authors report that the surface of a biocompatible hydrogel material, poly(vinyl alcohol) (PVA) can be altered by exposing it to reactive ion plasma (RIP) in order to increase primary endothelial cell attachment. The power and the carrier gas of the RIP treatment are varied and the resultant surface nitrogen, water contact angle, as well as the ability of the RIP‐treated surfaces to support primary endothelial colony forming cells is characterized. Additionally, …

Odontogenic Abscesses In Rhesus Macaques (Macaca Mulatta) Of Cayo Santiago, Hong Li, Wenjing Luo, Anna Feng, Michelle L. Tang, Terry B. Kensler, Elizabeth Maldonado, Octavio A. Gonzalez, Matthew J. Kessler, Paul C. Dechow, Jeffrey L. Ebersole, Qian Wang

Center for Oral Health Research Faculty Publications

Objectives
Odontogenic abscesses are one of the most common dental diseases causing maxillofacial skeletal lesions. They affect the individual's ability to maintain the dental structures necessary to obtain adequate nutrition for survival and reproduction. In this study, the prevalence and pattern of odontogenic abscesses in relation to age, sex, matriline, and living periods were investigated in adult rhesus macaque skeletons of the free-ranging colony on Cayo Santiago, Puerto Rico.

Materials and Methods
The skulls used for this study were from the skeletons of 752 adult rhesus macaques, aged 8–31 years, and born between 1951 and 2000. They came from 66 …

Anti-Apoptotic Effects Of Human Placental Hydrolysate Against Hepatocyte Toxicity In Vivo And In Vitro, Dong Ho Bak, Jungtae Na, Mi Ji Choi, Byung Chul Lee, Chang Taek Oh, Jeom Yong Kim, Hae Jung Han, Moo Joong Kim, Tae Ho Kim, Beom Joon Kim

Biological Sciences Faculty Publications

Apoptosis and oxidative stress are essential for the pathogenesis of acute liver failure and fulminant hepatic failure. Human placental hydrolysate (hPH) has been reported to possess antioxidant and anti-inflammatory properties. In the present study, the protective effects of hPH against D-galactosamine (D-GalN)- and lipopolysaccharide (LPS)-induced hepatocyte apoptosis were investigated in vivo. In addition, the molecular mechanisms underlying the anti-apoptotic activities of hPH against D-GalN-induced cell death in vitro were examined. Male Sprague-D awley rats were injected with D-GaIN/LPS with or without the administration of hPH. Rats were sacrificed 24 h after D-GaIN/LPS intraperitoneal injection, and the blood and liver samples …

Evaluation Of Three Forest-Based Bioenergy Development Strategies In The Inland Northwest, United States, Jill Moroney

Human-Environment Systems Research Center Faculty Publications and Presentations

In this article, we compare three bioenergy scenarios that use woody biomass from US Inland Northwest forests. The scenarios are based on current bioenergy research, development efforts, and stakeholder input. They include a small-scale system that produces drop-in transportation biofuel and biochar, a large, regional system that produces bio-aviation fuel, and a midsized pellet production system. We modeled woody biomass harvest, processing, and transportation, and then evaluated profitability and potential socioeconomic impacts to determine the overall viability of each strategy. Through interviews, we found widespread stakeholder support for all three scenarios. Wood-pellet production was profitable and feasible with current prices …

November 1, 2018, James Madison University

The Breeze, 2010-2019

The Breeze is the student newspaper of James Madison University in Harrisonburg, Virginia.

Trex1 Is Expressed By Microglia In Normal Human Brain And Increases In Regions Affected By Ischemia, Parul H Kothari, Grant R Kolar, Joanna C Jen, Rula Hajj-Ali, Paula Bertram, Robert E Schmidt, John P Atkinson

Open Access Publications

BACKGROUND: Mutations in the three-prime repair exonuclease 1 (TREX1) gene have been associated with neurological diseases, including Retinal Vasculopathy with Cerebral Leukoencephalopathy (RVCL). However, the endogenous expression of TREX1 in human brain has not been studied.

METHODS: We produced a rabbit polyclonal antibody (pAb) to TREX1 to characterize TREX1 by Western blotting (WB) of cell lysates from normal controls and subjects carrying an RVCL frame-shift mutation. Dual staining was performed to determine cell types expressing TREX1 in human brain tissue. TREX1 distribution in human brain was further evaluated by immunohistochemical analyses of formalin-fixed, paraffin-embedded samples from normal controls and patients …

Urban Re-Greening: A Case Study In Multi-Trophic Biodiversity And Ecosystem Functioning In A Post-Industrial Landscape, Frank Gallagher, Nina M. Goodey, Diane Hagmann, Jay Prakash Singh, Claus Holzapfel, Megan Litwhiler, Jennifer Adams Krumins

Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works

The biodiversity of urban and post-industrial ecosystems is a highly relevant and growing new frontier in ecological research. Even so, the functionality of these ecosystems may not always be successfully predicted based on prior biodiversity and ecosystem functioning theory. Indeed, evidence suggests that the general biological impoverishment within the urban context envisioned thirty years ago was overstated. Many of the world’s urban centers support some degree of biodiversity that is indigenous, as well as a complex array of non-native species, resulting in highly functional, and often, novel communities. For over two decades, a multi-disciplinary team has examined the sub-lethal impact …

Biological Biomedical Seminar | Thermal Adaptation In Lizard Communities, Nov. 5, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Ecolunch | Brain Endocasts As Proxies For Internal Neuroanatomy And Functional Capabilities In Extinct Birds, Nov. 7, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Info Session Tropical Diseases Research And Service Learning In Ecuador, Nov. 7, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Library Series | How Do Biologists Predict How Species Will Respond To Global Climate Change? Nov. 14, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Isu Today- November 30, 2018, Indiana State University, Communications And Marketing

University Announcements - ISU Today

No abstract provided.

Alternative Methods To Traditional Written Exam-Based Assessment, Shane Dillon, Alan Mcdonnell, Diarmaid Murphy, Lynsey White

Practitioner Research Projects

Donnelly and Fitzmaurice (2005, p.14) suggest that “assessment methods should be in accord with the learning outcomes of the module and should foster a deep approach to learning”. While unseen written exams do develop skills such as “examination techniques, writing under pressure, recall” (Smyth, 2004), there are a number of alternative assessment methods that determine what students actually understand and what they can do, in contrast to what they can recall. As one student noted “I hate to say it, but what you have got to do is to have a list of “facts” …you write down the important points …

Riparian Research And Management: Past, Present, Future: Volume 1, Steven W. Carothers, Hisham El Waer, Helen C. Fairley, Deborah M. Finch, Suzanne C. Fouty, Jonathan M. Friedman, Jennifer K. Frey, Stanley V. Gregory, Robert H. Hamre, Annie Henry, Osvel Hinojosa-Huerta, Elaine E. Johnson, R. Roy Johnson, Kenneth J. Kingsley, Mary Anne Mcleod, Eric Mellink, Katie Merewether, Duncan T. Patten, John S. Richardson, Anne Sands, Michael L. Scott, Bo Shelby, Anna A. Sher, D. Max Smith, John T. Stanley, Frederick J. Swanson, Raymond M. Turner, Robert H. Webb, William E. Werner

Watershed Sciences Faculty Publications

Fifty years ago, riparian habitats were not recognized for their extensive and critical contributions to wildlife and the ecosystem function of watersheds. This changed as riparian values were identified and documented, and the science of riparian ecology developed steadily. Papers in this volume range from the more mesic northwestern United States to the arid Southwest and Mexico. More than two dozen authors—most with decades of experience—review the origins of riparian science in the western United States, document what is currently known about riparian ecosystems, and project future needs. Topics are widespread and include: interactions with fire, climate change, and declining …

Und Today: November 2018, University Of North Dakota

UND Today Archive

No abstract provided.

Resource Assessment Report Temperate Demersal Elasmobranch Resource Of Western Australia, Matias Braccini, Nick Blay, S. A. Hesp, Brett Molony

Fisheries Research Reports

Whiskery (Furgaleus macki), gummy (Mustelus antarcticus), dusky (Carcharhinus obscurus) and sandbar (C. plumbeus) sharks are the main (~80% of the shark catch) shark species of the Temperate Demersal Elasmobranch Resource (TDER).

Improving Online Interactions: Lessons From An Online Anatomy Course With A Laboratory For Undergraduate Students, Stephanie M. Attardi, Michele L. Barbeau, Kem A. Rogers

Anatomy and Cell Biology Publications

An online section of a face-to-face (F2F) undergraduate (bachelor's level) anatomy course with a prosection laboratory was offered in 2013-2014. Lectures for F2F students (353) were broadcast to online students (138) using Blackboard Collaborate (BBC) virtual classroom. Online laboratories were offered using BBC and three-dimensional (3D) anatomical computer models. This iteration of the course was modified from the previous year to improve online student-teacher and student-student interactions. Students were divided into laboratory groups that rotated through virtual breakout rooms, giving them the opportunity to interact with three instructors. The objectives were to assess student performance outcomes, perceptions of student-teacher and …

Pannexin 1 Regulates Adipose Stromal Cell Differentiation And Fat Accumulation, Vanessa R. Lee, Kevin J. Barr, John J. Kelly, Danielle Johnston, Cody F.C. Brown, Kevin P. Robb, Samar Sayedyahossein, Kenneth Huang, Robert Gros, Lauren E. Flynn, Silvia Penuela

Anatomy and Cell Biology Publications

Pannexin 1 (Panx1) is a channel-forming glycoprotein important in paracrine signaling and cellular development. In this study, we discovered that mice globally lacking Panx1 (KO) have significantly greater total fat mass and reduced lean mass compared to wild type (WT) mice under a normal diet. Despite having higher fat content, Panx1 KO mice on a high fat diet exhibited no differences in weight gain and blood markers of obesity as compared to WT controls, except for an increase in glucose and insulin levels. However, metabolic cage data revealed that these Panx1 KO mice display significantly increased activity levels, higher ambulatory …

Response To The Growing Dementia Burden Must Be Broader, Werner Hacke, Vladimir Hachinski

Anatomy and Cell Biology Publications

No abstract provided.