Digital Commons Network^™

Non-Canonical Pi3’Kinase Signaling Regulates Akt3 Activity In Pten-Null Basal Breast Cancer Cell Lines, Ericka Lashonda Smith

MUSC Theses and Dissertations

A subset of basal breast cancer patients has tumors overexpressing EGFR, loss of PTEN expression and mutated p53; these alterations cooperate and result in a poor prognosis for these patients. PTEN is a direct negative regulator of PI3’Kinase enzymatic activity and loss of PTEN expression is correlated with elevated PI3’Kinase pathway activation. In the presented dissertation, we utilized a panel of PTEN-null basal breast cancer cell lines containing constitutive AKT activation and elevated levels of EGFR to model this subset of breast cancer patients. We first investigated the response of AKT phosphorylation to PTEN re-expression in the cell line panel. …

Synthesis And Characterization Of Novel Bioplastics By Innovative 3d Printing Approaches, Kathryn Marie Hall

Theses and Dissertations

This work describes the synthesis and characterization of novel bioplastic composites by using novel additive manufacturing (AM) approaches. As a background, global plastic consumption has become a problem with the accumulation of plastics in landfills and the ocean. Recently, bioplastics have emerged as a viable option to petrochemicals. Currently, there are some issues like biodegradability, property optimization with bioplastics etc. In thesis, novel methods and engineering of composition of bioplastics are being reported. In Chapter II, the addition of lignin to the PLA polymer matrix by using solvent mixing will be reported. During this study, liquid ink mixture was successfully …

Stroop And Mindfulness: An Experimental Multi-Group Comparison, Anna Lauren Schimmelpfennig

Theses and Dissertations

Mindfulness has been associated with various benefits, including enhanced cognitive performance and disengagement from problematic emotions. Previous research has indicated that engaging in a brief mindfulness exercise may enhance performance on the Color Word Stroop, neutral Stroop, and emotional Stroop. Participants in the current study were 201 students (139 female), mean age 19.7 years (SD=1.4) from a Midwestern university, who were randomly assigned to listen to a 9- minute mindfulness exercise, progressive muscle relaxation (PMR) exercise, or interview control condition before completing the Color-Word Stroop, Neutral Stroop, and Emotional Stroop. Preliminary analysis reveal that participants generally had positive attitudes toward …

Fourth Amendment Anxiety, Kiel Brennan-Marquez, Stephen E. Henderson

Faculty Articles

In Birchfield v. North Dakota (2016), the Supreme Court broke new Fourth Amendment ground by establishing that law enforcement’s collection of information can be cause for “anxiety,” meriting constitutional protection, even if subsequent uses of the information are tightly restricted. This change is significant. While the Court has long recognized the reality that police cannot always be trusted to follow constitutional rules, Birchfield changes how that concern is implemented in Fourth Amendment law, and importantly, in a manner that acknowledges the new realities of data-driven policing. Beyond offering a careful reading of Birchfield, this Article has two goals. First, …

Physician-Assisted Death With Dignity: Expanding Palliative Care In Rhode Island, Camille Caron Capraro

Roger Williams University Law Review

No abstract provided.

Elucidation The Toxicity Mechanism Of Zinc Oxide Nanoparticle Using Molecular Docking Approach With Proteins, Krishna Pal Singh, Anupam Dhasmana, Qamar Rahman

School of Medicine Publications

Objective: At present, toxicological tests are resource-intensive, time-consuming and require a large pool of animal models for toxicity assessment. To speed up the toxicity evaluation and to reduce animal suffering during toxicity assessment, the use of alternative methods including computational models is in high demand. The computational toxicity prediction methods are very helpful for the regulatory bodies to quickly assess the health impact of nanomaterial materials. In the present work, we have examined the mechanism of zinc oxide nanoparticle (ZnO-NP)-proteins interaction and their effect of surface chemistries of ZnO-NP on the bioactive conformation of chemokines and other cytological proteins using …

Laboratory Focus On Improving The Culture Of Biosafety: Statewide Risk Assessment Of Clinical Laboratories That Process Specimens For Microbiologic Analysis, Erik Munson, Erin J. Bowles, Richard Dern, Eric Beck, Raymond P. Podzorski, Allen C. Batemman, Timothy K. Block, Joshua L. Kropp, Tyler Radke, Karen Siebers, Brian Simmons, Mary A. Smith, Frances Spray-Larson, David M. Warshauer

Clinical Lab Sciences Faculty Research and Publications

The Wisconsin State Laboratory of Hygiene challenged Wisconsin laboratories to examine their biosafety practices and improve their culture of biosafety. One hundred three clinical and public health laboratories completed a questionnaire-based, microbiology-focused biosafety risk assessment. Greater than 96% of the respondents performed activities related to specimen processing, direct microscopic examination, and rapid nonmolecular testing, while approximately 60% performed culture interpretation. Although they are important to the assessment of risk, data specific to patient occupation, symptoms, and travel history were often unavailable to the laboratory and, therefore, less contributory to a microbiology-focused biosafety risk assessment than information on the specimen source …

A Preliminary Investigation Of Acculturative Stress And Diurnal Cortisol Among Latina Women, Lucas Torres, Felicia Mata-Greve, April L. Harkins

Clinical Lab Sciences Faculty Research and Publications

The interplay between biological and cultural factors has remained understudied among ethnic minority groups, including Latinos. To the best of our knowledge, minimal to no research has examined the relationship between acculturative stressors and diurnal cortisol among Latina women. This initial exploratory study sought to examine the relationship between cortisol and acculturative stress. Among a small sample of adult Latina women, salivary cortisol was collected at 3 time points (waking, 30 min postwaking, and bedtime) to assess the cortisol awakening response (CAR) and diurnal cortisol response. Information regarding acculturative stress and acculturation was also collected. The major results showed that …

Use Of Hyperspectral Images (Hsi) And Convolutional Neural Network (Cnn) To Identify Normal, Precancerous And Cancerous Tissues, Pallavi Jain

Dissertations

Cancer detection has been a great topic of research for a long time, as early detection of cancer can help in increasing the survival rate of patients by providing on time better treatment. A robust system is required in order to detect early-stage cancer as its difficult to identify early-stage cancer from the normal clinical process. The computer vision techniques provide a new way to understand the challenges related to the medical image analysis. This thesis presents the medical image analysis using a combination of Convolutional Neural Network and Hyperspectral Images of cancer patient's tissues. The idea behind choosing the …

Amphibians And Reptiles Of The State Of San Luis Potosi, Mexico, With Comparisons With Adjoining States, J. A. Lemos-Espinal, Geoffrey R. Smith, G. A. Woolrich-Pina

Faculty Publications

No abstract provided.

Amphibians And Reptiles Of The State Of Durango, Mexico, With Comparisons With Adjoining States, J. A. Lemos-Espinal, Geoffrey R. Smith, H. Gadsden-Esparza, R. Valdez-Lares, G. A. Woolrich-Pina

Faculty Publications

No abstract provided.

Growth, Fitness, And Overwinter Survival Of A Shattercane (Sorghum Bicolor Ssp. Drummondii) × Grain Sorghum (Sorghum Bicolor Ssp. Bicolor) F2 Population, Jared J. Schmidt, Melinda K. Yerka, Jeffrey F. Pedersen, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Although sorghum [Sorghum bicolor (L.) Moench ssp. bicolor] is the fifth most important grain crop in terms of global production, no commercial hybrids carry genetically engineered (GE) traits for resistance to insect pests or herbicides due to regulatory concerns about gene flow to weedy relatives. However, non-GE herbicide resistance currently is being developed in grain sorghum and will likely transfer to related weeds. Monitoring the impact of this new nuclear technology on the evolution and invasiveness of related weeds requires a baseline understanding of the population biology of grain sorghum genes once they transfer to in situ weed …

Phenotypic Data From Inbred Parents Can Improve Genomic Prediction In Pearl Millet Hybrids, Zhikai Liang, Shashi K. Gupta, Cheng-Ting Yeh, Yang Zhang, Daniel W. Ngu, Ramesh Kumar, Hemant T. Patil, Kanulal D. Mungra, Dev Vart Yadav, Abhishek Rathore, Rakesh K. Srivastava, Rajeev Gupta, Jinliang Yang, Rajeev K. Varshney, Patrick S. Schnable, James C. Schnable

Department of Agronomy and Horticulture: Faculty Publications

Pearl millet is a non-model grain and fodder crop adapted to extremely hot and dry environments globally. In India, a great deal of public and private sectors’ investment has focused on developing pearl millet single cross hybrids based on the cytoplasmic-genetic male sterility (CMS) system, while in Africa most pearl millet production relies on open pollinated varieties. Pearl millet lines were phenotyped for both the inbred parents and hybrids stage. Many breeding efforts focus on phenotypic selection of inbred parents to generate improved parental lines and hybrids. This study evaluated two genotyping techniques and four genomic selection schemes in pearl …

Isolation And Analysis Of Microbial Communities In Soil, Rhizosphere, And Roots In Perennial Grass Experiments, Morgan R. Mcpherson, Peng Wang, Robert B. Mitchell, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Plant and soil microbiome studies are becoming increasingly important for understanding the roles microorganisms play in agricultural productivity. The purpose of this manuscript is to provide detail on how to rapidly sample soil, rhizosphere, and endosphere of replicated field trials and analyze changes that may occur in the microbial communities due to sample type, treatment, and plant genotype. The experiment used to demonstrate these methods consists of replicated field plots containing two, pure, warm-season grasses (Panicum virgatum and Andropogon gerardii) and a low-diversity grass mixture (A. gerardii, Sorghastrum nutans, and Bouteloua curtipendula). Briefly, plants …

Hunger In The Land Of Plenty: Local Responses To Food Insecurity In Iowa, Gabrielle E. Roesch-Mcnally, Jacqueline Ann Nester, Andrea D. Basche, Eric Christianson, Emily Zimmerman

Department of Agronomy and Horticulture: Faculty Publications

Story County (estimated population 92,406 in 2013) lies in the heart of central Iowa, a state renowned for its remarkable agricultural productivity. Iowa leads all states for production of corn, soybean, and hogs. Revenues from agricultural products in Iowa total more than $30 billion annually according the 2012 Agricultural Census (USDA-NASS 2014). This productivity stems from a favorable natural and political environment. The temperate climate, productive soils, and gentle topography are ideal for our production system of commodity agriculture facilitated by federal policies, which include subsidized crop insurance and commodity payments (Horrigan, Lawrence, and Walker 2002). Despite this productivity and …

Social–Ecological Landscape Patterns Predict Woody Encroachment From Native Tree Plantings In A Temperate Grassland, Victoria M. Donovan, Jessica L. Burnett, Christine H. Bielski, Hannah E. Birge, Rebecca Bevans, Dirac L. Twidwell Jr, Craig R. Allen

Department of Agronomy and Horticulture: Faculty Publications

Afforestation is often viewed as the purposeful planting of trees in historically non-forested grasslands, but an unintended consequence is woody encroachment, which should be considered part of the afforestation process. In North America’s temperate grassland biome, Eastern redcedar (Juniperus virginiana L.) is a native species used in tree plantings that aggressively invades in the absence of controlling processes. Cedar is a well-studied woody encroacher, but little is known about the degree to which cedar windbreaks, which are advocated for in agroforestry programs, are contributing to woody encroachment, what factors are associated with cedar spread from windbreaks, nor where encroachment …

Engineering Linear, Branched-Chain Triterpene Metabolism In Monocots, Chase Kempinski, Zuodong Jiang, Garrett Zinck, Shirley J. Sato, Zhengxiang Ge, Thomas E. Clemente, Joe Chappell

Department of Agronomy and Horticulture: Faculty Publications

Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to yield farnesyl diphosphate (FPP), catalyzed by FPP synthase (FPS). Squalene synthase (SQS) condenses two molecules of FPP to generate the symmetrical product squalene, the first committed precursor to sterols and most other triterpenes. In the green algae Botryococcus braunii, two FPP molecules can also be condensed in an asymmetric manner yielding the more highly branched triterpene, botryococcene. Botryococcene …

Genomic Selection In Preliminary Yield Trials In A Winter Wheat Breeding Program, Vikas Belamkar, Mary J. Guttieri, Waseem Hussain, Diego Jarquin, Ibrahim El-Basyoni, Jesse Poland, Aaron J. Lorenz, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Genomic prediction (GP) is now routinely performed in crop plants to predict unobserved phenotypes. The use of predicted phenotypes to make selections is an active area of research. Here, we evaluate GP for predicting grain yield and compare genomic and phenotypic selection by tracking lines advanced. We examined four independent nurseries of F_3:6 and F_3:7 lines trialed at 6 to 10 locations each year. Yield was analyzed using mixed models that accounted for experimental design and spatial variations. Genotype-by-sequencing provided nearly 27,000 high-quality SNPs. Average genomic predictive ability, estimated for each year by randomly masking lines as missing …

Transient Heat Stress During Early Seed Development Primes Germination And Seedling Establishment In Rice, Kevin Begcy, Jaspreet Sandhu, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Rice yield is highly sensitive to increased temperature. Given the trend of increasing global temperatures, this sensitivity to higher temperatures poses a challenge for achieving global food security. Early seed development in rice is highly sensitive to unfavorable environmental conditions. Heat stress (HS) during this stage decreases seed size and fertility, thus reducing yield. Here, we explore the transgenerational phenotypic consequences of HS during early seed development on seed viability, germination, and establishment. To elucidate the impact of HS on the developmental events in postzygotic rice seeds, we imposed moderate (35°C) and severe (39°C) HS treatments initiated 1 day after …

Feelings Of Enlightenment: A Hermeneutic Interpretation Of Latent Enlightenment Assumptions In Greenberg's Emotion-Focused Therapy, Alex A. Gomez

Antioch University Dissertations & Theses

The purpose of this dissertation is to explore how a mainstream theory of psychological practice might inadvertently conceal and ignore contemporary values and ideologies and their pathological consequences. Through a hermeneutic approach, I interpreted Leslie Greenberg’s Emotion-focused therapy: Coaching clients to work through their feelings (2^nd ed), a popular and widely used theory in psychotherapy. As a practitioner with humanistic foundations, this was also an opportunity for the author to understand his own unexamined values as a therapist. Specific EFT constructs and concepts that reflected Enlightenment assumptions and values were examined. EFT was situated within Enlightenment philosophy, particularly …

Modeling Oculomotor Variability During Slow Cabin Decompression Using Infrared Technology, Jennifer E. Thropp, Paul W. Buza

International Journal of Aviation, Aeronautics, and Aerospace

Slow cabin decompression is highly perilous due to its gradual and thus generally inconspicuous nature. In an applied approach to studying its effects upon oculomotor performance, experienced pilots performed a simulated flight task during slow decompression inside a high-altitude chamber while an infrared-based head-mounted eye-tracker measured oculomotor and pupillary changes at approximately 5% decrements in blood oxygen saturation. Saccade angle, saccade duration, saccadic velocity, microsaccade rate, fixation duration, and pupil diameter varied systematically in response to manipulation of blood oxygen saturation level, including recovery upon return to normoxia. Changes in these oculomotor variables can serve as biomarkers for early hypoxia …

Mental Health In Commercial Aviation - Depression & Anxiety Of Pilots, Maurice C. Dehoff, Stephen K. Cusick

International Journal of Aviation, Aeronautics, and Aerospace

Two landmark accidents invigorated the discussion regarding pilot mental health. Other incidents may also have a mental health component that is related to, but not the direct cause of, the event. It is suspected that these “other incidents” are much more commonplace and while not catastrophic, erode safety. Depression and anxiety are two commonly occurring mental health concerns. Research indicates that a considerable portion of the U. S. population suffers from some form of mental health issue. Most go undiagnosed and untreated. A similar rate of occurrence appears to be found within the airline pilot population. The stigma of mental …

Deep Recurrent Learning For Efficient Image Recognition Using Small Data, Mahbubul Alam

Electrical & Computer Engineering Theses & Dissertations

Recognition is fundamental yet open and challenging problem in computer vision. Recognition involves the detection and interpretation of complex shapes of objects or persons from previous encounters or knowledge. Biological systems are considered as the most powerful, robust and generalized recognition models. The recent success of learning based mathematical models known as artificial neural networks, especially deep neural networks, have propelled researchers to utilize such architectures for developing bio-inspired computational recognition models. However, the computational complexity of these models increases proportionally to the challenges posed by the recognition problem, and more importantly, these models require a large amount of data …

Plasma Medicine: A Brief Introduction, Mounir Laroussi

Electrical & Computer Engineering Faculty Publications

This mini review is to introduce the readers of Plasma to the field of plasma medicine. This is a multidisciplinary field of research at the intersection of physics, engineering, biology and medicine. Plasma medicine is only about two decades old, but the research community active in this emerging field has grown tremendously in the last few years. Today, research is being conducted on a number of applications including wound healing and cancer treatment. Although a lot of knowledge has been created and our understanding of the fundamental mechanisms that play important roles in the interaction between low temperature plasma and …

Who Is Caring For The Caregiver? The Role Of Cybercoping For Dementia Caregivers, Jae-Seon Jeong, Young Kim, Myoung-Gi Chon

College of Communication Faculty Research and Publications

The purpose of this study is to investigate the relationship between dementia caregivers’ communication behaviors (information seeking and forwarding) and their outcomes (coping outcomes: e.g., dealing better with negative feelings or improved medical outcomes). A survey data set of dementia patients’ caregivers substantiates the effects of communication behaviors about dementia illness on coping outcomes, as well as the mediating role of emotion-focused and problem-focused coping processes. Using structural equation modeling (SEM), this study found positive effects of communication behaviors on outcomes through coping processes. Further, the results indicate that communication behaviors in cyberspace are crucial for caregivers to cope with …

Study Of Low Dose Nitrite Stress On Human Mononuclear Cells: An In-Vitro Case-Control Study On Osteoarthritis, Junu Shrestha

Dissertations and Theses @ UNI

Nitrate, (NOR 3RP - P) is an oxidized form of nitrogen and it is an integral part of the nitrogen cycle in the natural environment. Nitrates are extensively used in agricultural practices as fertilizers, applied as either animal manures or derived slurries or as chemical fertilizers in the form of ammonium nitrate. Most of the toxicologically relevant nitrate enters the human body through consumption of vegetables, fruits, processed food products, and drinking water. In the ground water system, nitrates are a problematic and widespread contaminant, especially in agricultural areas such as the Midwest, South, and Northeastern U.S. Low-levels of nitrate …

Blood Coagulation Properties In Hibernating And Non-Hibernating American Bullfrogs (Lithobates Catesbeianus), Stephen Cain

Dissertations and Theses @ UNI

Activated partial thromboplastin times (APTT) and factor deficiency assays were performed on the plasma of twenty American bullfrogs (Lithobates catesbeianus) to test for potential changes and regulation of the intrinsic coagulation pathway during and after hibernation. Bullfrogs (pre-hibernating n=4, hibernating n=6, post-hibernating n=10) were housed in 10 gallon aquariums filled with RO water and placed in a temperaturecontrolled room. Hibernation was gradually induced by decreasing the temperature of the room from 24 C to 3 C in increments of 3 C per week. Blood (6-8 ml) was collected from individual non-hibernating frogs, hibernating frogs and post-hibernating frogs in 0.105M sodium …

Synthesis And Characterization Of Glycolipids, Glycoclusters And Glycomacrocycles, Anji Chen

Chemistry & Biochemistry Theses & Dissertations

Low molecular-weight gelators (LMWGs) are a fascinating and interesting class of compounds that can self-assemble and form supramolecular structures in appropriate solvents. The gelators form fibrous networks which entrap the solvent and result in a supramolecular gel. Carbohydrate-derived LMWGs are especially useful since they are more likely to be biocompatible and biodegradable. Dendritic gelators are branch-shaped molecules with precise molecular weight that are between small molecules and polymer gelators. Glycoclusters with sugar moieties at the periphery of the molecules are important classes of bioactive compounds due to the multivalency effect. Lower generation glycoclusters are relatively small branched systems that have …

It Takes A Village-Schooling Out Of Place: School Experiences Of Black African Youth In Waterloo Region, Olufunke Oba 5726040

Theses and Dissertations (Comprehensive)

This dissertation examined experiences of alienation among Black African youth in Waterloo region schools. This study is timely and relevant considering the rapid influx of Black Africans into the region in response to government initiatives to redirect immigrants to smaller communities. The research addressed the dearth of scholarship on experiences of Black Africans outside the major metropolitan areas by employing Afrocentric and critical race theories to explore relationships between race and youth experiences of alienation. The dissertation study utilized elder facilitated youth dialogue forum (adaptation of focus group) and in-depth storytelling (which honours African orality) to access the meanings seventeen …

The Hold Me Tight Program For Couples Becoming Parents: A Mixed Methods Study, Debbie Wang

Theses and Dissertations (Comprehensive)

ABSTRACT

Attachment theory has made substantial contributions to the understanding of close relationships. The purpose of this study was to inquire whether an attachment-informed psychoeducational program is a feasible and effective intervention for couples expecting their first child. The overarching question was: Is an attachment-informed relationship enhancement program, such as Hold Me Tight^® (HMT), helpful to couples in strengthening their relationship and increasing their confidence in becoming first-time parents? The research question was addressed using a mixed-methods approach.

In the first phase, the Hold Me Tight^® program developed by Dr. Sue Johnson was modified for use with couples …