Digital Commons Network^™

Killing The Policy To Save The Child: Comparing The Historical Removal Of Indigenous Children In Austrailia To The United States And How The Countries Can Learn From Each Other, Drew Pollom

American Indian Law Journal

No abstract provided.

Investigating The Effects Of Copy Number Variants On Reading And Language Performance, Alessandro Gialluisi, Alessia Visconti, Erik G. Willcutt, Shelley D. Smith, Bruce F. Pennington, Mario Falchi, John C. Defries, Richard K. Olson, Clyde Francks, Simon E. Fisher

Psychology: Faculty Scholarship

Background

Reading and language skills have overlapping genetic bases, most of which are still unknown. Part of the missing heritability may be caused by copy number variants (CNVs).

Methods

In a dataset of children recruited for a history of reading disability (RD, also known as dyslexia) or attention deficit hyperactivity disorder (ADHD) and their siblings, we investigated the effects of CNVs on reading and language performance. First, we called CNVs with PennCNV using signal intensity data from Illumina OmniExpress arrays (~723,000 probes). Then, we computed the correlation between measures of CNV genomic burden and the first principal component (PC) score …

Revelation Of Yin-Yang Balance In Microbial Cell Factories By Data Mining, Flux Modeling, And Metabolic Engineering, Gang Wu

McKelvey School of Engineering Graduate Student Theses & Dissertations

The long-held assumption of never-ending rapid growth in biotechnology and especially in synthetic biology has been recently questioned, due to lack of substantial return of investment. One of the main reasons for failures in synthetic biology and metabolic engineering is the metabolic burdens that result in resource losses. Metabolic burden is defined as the portion of a host cells resources either energy molecules (e.g., NADH, NADPH and ATP) or carbon building blocks (e.g., amino acids) that is used to maintain the engineered components (e.g., pathways). As a result, the effectiveness of synthetic biology tools heavily dependents on cell capability to …

Mechanics Of Early Retina And Lens Development In The Embryo, Alina Oltean

McKelvey School of Engineering Graduate Student Theses & Dissertations

Mechanical forces play an essential role in morphogenesis, the shaping of embryonic structures. This research focuses mainly on eye development, a problem that has been studied for decades using a variety of approaches. However, the mechanics of the early stages of eye formation remain incompletely understood.

The embryonic eyes begin as bilateral protrusions called optic vesicles (OVs) that grow outward from the anterior end of the brain tube. The optic vesicles contact and adhere to the overlying surface ectoderm (SE) via extracellular matrix (ECM). Then, both layers thicken in the region of contact to form the retinal and lens placodes, …

Feed-Forward Inhibitory Circuits In Hippocampus And Their Computational Role In Fragile X Syndrome, Sarah Lauren Wahlstrom Helgren

McKelvey School of Engineering Graduate Student Theses & Dissertations

Feed-forward inhibitory (FFI) circuits are canonical neural microcircuits. They are unique in that they are comprised of excitation rapidly followed by a time-locked inhibition. This sequence provides for a powerful computational tool, but also a challenge in the analysis and study of these circuits. In this work, mechanisms and computations of two hippocampal FFI circuits have been examined. Specifically, the modulation of synaptic strength of the excitation and the inhibition is studied during constant-frequency and naturalistic stimulus patterns to reveal how FFI circuit properties and operations are dynamically modulated during ongoing activity. In the first part, the FFI circuit dysfunction …

Goggle Augmented Imaging And Navigation System For Fluorescence-Guided Surgery, Suman Bikash Mondal

McKelvey School of Engineering Graduate Student Theses & Dissertations

Surgery remains the only curative option for most solid tumors. The standard-of-care usually involves tumor resection and sentinel lymph node biopsy for cancer staging. Surgeons rely on their vision and touch to distinguish healthy from cancer tissue during surgery, often leading to incomplete tumor resection that necessitates repeat surgery. Sentinel lymph node biopsy by conventional radioactive tracking exposes patients and caregivers to ionizing radiation, while blue dye tracking stains the tissue highlighting only superficial lymph nodes. Improper identification of sentinel lymph nodes may misdiagnose the stage of the cancer. Therefore there is a clinical need for accurate intraoperative tumor and …

Cyclotron Production And Biomedical Imaging Applications Of The Pet Isotope Manganese-52, Andrew Lake Wooten

McKelvey School of Engineering Graduate Student Theses & Dissertations

Manganese is an important element for biomedical research because of its roles as an essential micronutrient and as a neurotoxin from chronic elevated exposure, as well as the role of manganese(II) as a paramagnetic core for contrast agents in T1-weighted magnetic resonance imaging (MRI). Using a radiotracer of manganese provides excellent sensitivity for studying these phenomena, but only 52Mn met the criteria for our experiments: (1) a half-life (t1/2=5.6 days) that was long enough to examine timepoints over several days, (2) a half-life that was short enough to emit sufficient counts for a realistic scan time, and (3) emitted radiation …

New Zealand And Climate Change: What Are The Stakes And What Can New Zealand Do?, Ig Bailey, Inderberg Th Jackson

School of Geography, Earth and Environmental Sciences

No abstract provided.

Wilde's Final Act, Justine Ilissa Sha

Seton Hall University Dissertations and Theses (ETDs)

This thesis examines Oscar Wilde’s De Profundis, the prison-written letter to his ex-lover, as a work created with the signature Wildean dramatic conventions of composition, style, the writing process, and formal structure of which make up Wilde’s theatrical plays “Lady Windermere’s Fan” and “The Importance of Being Earnest.” This shows how De Profundis was written not as an autobiographical work, a love letter, or a portrait of a tormented, imprisoned mind, but as a performance: paradoxical play in the same style as his dramatic repertoire, and desperately intended for publication. First, the Wilde’s dramatic writing process is examined. Then, using …

Development Of Steady-State And Dynamic Flux Models For Broad-Scope Microbial Metabolism Analysis, Lian He

McKelvey School of Engineering Graduate Student Theses & Dissertations

Flux analysis techniques, including flux balance analysis (FBA) and 13C-metabolic flux analysis (MFA), can characterize carbon and energy flows through a cells metabolic network. By employing both 13C-labeling experiments and nonlinear programming, 13C-MFA provides a rigorous way of examining cell flux distributions in the central metabolism. FBA, on the other hand, gives a holistic review of optimal fluxomes on the genome scale. In this dissertation, flux analysis techniques were constructed to investigate the microbial metabolisms.

First, an open-source and programming-free platform of 13C-MFA (WUFlux) with a user-friendly interface in MATLAB was developed, which allowed both mass isotopomer distribution (MID) analysis …

Mechanisms Of Diabetes-Induced Liver Damage : The Role Of Oxidative Stress And Inflammation (, Jamaludin Mohamed, Nazratun Nafizah A. H, Zariyantey A. B, Budin S.

Sultan Qaboos University Medical Journal

Diabetes mellitus is a non-communicable disease that occurs in both developed and developing countries. This metabolic disease affects all systems in the body, including the liver. Hyperglycaemia, mainly caused by insulin resistance, affects the metabolism of lipids, carbohydrates and proteins and can lead to nonalcoholic fatty liver disease, which can further progress to non-alcoholic steatohepatitis, cirrhosis and, finally, hepatocellular carcinomas. The underlying mechanism of diabetes that contributes to liver damage is the combination of increased oxidative stress and an aberrant inflammatory response; this activates the transcription of pro-apoptotic genes and damages hepatocytes. Significant involvement of pro-inflammatory cytokines—including interleukin (IL)-1β, IL-6 …

Double Alternating Minimization (Dam) For Phase Retrieval In The Presence Of Poisson Noise And Pixelation, Weimin Zhou

McKelvey School of Engineering Graduate Student Theses & Dissertations

Optical detectors, such as photodiodes and CMOS cameras, can only read intensity information, and thus phase information of wavefronts is lost. Phase retrieval algorithms are used to estimate the lost phase and reconstruct an accurate effective pupil function, where the squared modulus of its Fourier transform is detected by a camera. However, current algorithms such as the Gerchberg-Saxton algorithm and Fienup-style algorithm do not consider the detector sampling rate and shot noise introduced by photon detection. If the sampling rate is low, we must interpolate the detected image in order to accurately reconstruct its pupil function. Here, we develop an …

Environmental Fullerene Chemistry: Elucidating Critical Reaction Pathways And Resulting Products In The Aqueous Phase, Jiewei Wu

McKelvey School of Engineering Graduate Student Theses & Dissertations

As the production of fullerenes and fullerene-based materials approaches industrial scale, there are increasing interests/concerns regarding their (potential) environmental impact(s) upon release. To date, a number of critical, aqueous-based fullerene transformation pathways under environmentally relevant conditions remain poorly understood. Comprehensive, fundamental, and quantitative understanding of the potential (major) reaction pathways and resulting products of fullerene materials, particularly in aquatic systems, is now crucial for their accurate fate, transport, life cycle, risk assessment(s), and thus ultimate material sustainability.

Herein, this dissertation is focused on identifying and elucidating aqueous transformation pathways of fullerene materials, focused on C60 as a model fullerene, under …

Social Support And Intimate Partnerships In African American Women With Breast Cancer, Tess Thompson

Arts & Sciences Graduate Student Theses and Dissertations

This mixed methods study investigated social support in African American breast cancer patients in one Midwestern metropolitan area. The study includes a quantitative analysis of social support in 227 African American breast cancer patients participating in a quality of life study, as well as a qualitative component involving a subsample of 15 married participants. In the larger sample, growth curve models were used to assess factors contributing to initial levels of perceived social support and change in social support over time. Interviews with women in the qualitative study allowed an in-depth exploration of how breast cancer affected womens relationships with …

Four Essays In Education And Epidemiology, Brittni Danielle Jones

Arts & Sciences Graduate Student Theses and Dissertations

This dissertation includes four, co-authored essays in which social epidemiological concepts and methods were applied to explore the nexus of race, education, and health in the United States. In the first two essays, colleagues and I employed geospatial mapping and analysis to examine the association between past, racially segregative, housing practices and the geospatial distribution of poor, education, health, and related developmental outcomes across Ferguson, Missouri, and the surrounding St. Louis metropolitan region. We investigated this association during two, respective cross-sections of time. Results revealed the nature and distribution of class and racial disparities across the region. Based on the …

Photoenhanced Oxidation Of C60 In Water: Exploring The Roles Of H2o2 And Hydroxyl Radical Based Reactions, Yining Ou

McKelvey School of Engineering Graduate Student Theses & Dissertations

Transformation of water-stable C₆₀ clusters (nC₆₀) to oxidized, soluble C₆₀ derivatives via photoreactions (i.e., reactions in UV irradiation) and ground-state reactions (i.e., reactions in dark) with various oxidants, such as oxygen, ozone, free chlorine, among others, have been described as critical processes in understanding the ultimate environmental fate of fullerene-based materials. However, few studies have explored the oxidation of aqueous based C₆₀ (as water stable nC₆₀) with hydrogen peroxide (H₂O₂) / hydroxyl radical (^.OH), which are common oxidants in both natural and engineered systems. Herein, the aqueous …

Engineering Cre Recombinase For Genome Engineering, Chi Zhang

Arts & Sciences Graduate Student Theses and Dissertations

Cre recombinase recombines its DNA target, loxP sites, without help of accessory proteins or DNA repair systems. The simplicity of Cre-lox system has been widely utilized for genome editing, especially in mouse genetics. The goal of this dissertation is to constructCrerecombinase variants that will operate uponrecombination target sites (RTs) present within the genome, instead of perturbing the genome by inserting wildtype RTs for subsequent genome engineering. In general, the desired RTs native to the genome are asymmetric. However, the loxP sequence is pseudo-palindromic, requiring a homotetrameric formation of Cre recombinase. As a first step, I broke the symmetry of Cre …

Translational Photoacoustic Tomography, Yong Zhou

McKelvey School of Engineering Graduate Student Theses & Dissertations

Combining optical excitation and ultrasonic detection, photoacoustic tomography (PAT) offers deep imaging with high resolution. With optical excitation, PAT maintains the high contrast of optical imaging. Because of the low scattering of ultrasonic waves in tissue, PAT achieves high spatial resolution at depths. Several advantages make PAT suitable for clinical application, including its scalable penetration and resolution, high optical absorption contrast, fast imaging speed, and ability to perform spectral decomposition. Based on different image reconstruction mechanisms, PAT can be further divided into two embodiments: raster-scanning-based photoacoustic microscopy (PAM) and reconstruction-algorism-based photoacoustic computed tomography (PACT). This dissertation aims to advance the …

An Evolutionary Perspective On The Co-Occurrence Of Social Anxiety Disorder And Alcohol Use Disorder, A Bulley, B Miloyan, B Brilot, Mj Gullo, T Suddendorf

School of Psychology

Social Anxiety Disorder (SAD) commonly co-occurs with, and often precedes, Alcohol Use Disorder (AUD). In this paper, we address the relationship between SAD and AUD by considering how natural selection left socially anxious individuals vulnerable to alcohol use, and by addressing the underlying mechanisms. We review research suggesting that social anxiety has evolved for the regulation of behaviors involved in reducing the likelihood or consequences of threats to social status. The management of potential threats to social standing is important considering that these threats can result in reduced cooperation or ostracism - and therefore to reduced access to coalitional partners, …

A Quantitative Genetic Analysis Of Craniofacial Variation In Baboons, Jessica Lynn Joganic

Arts & Sciences Graduate Student Theses and Dissertations

This dissertation is an explication of baboon craniofacial variation and its genetic basis. Intraspecific variation is the result of input from and complex interactions among genetic information, functional demands, and developmental processes. The relative effect of each of these on craniofacial variation, as well as the degree of inter-trait covariance, determines whether traits can respond to selection and what that response might look like. Using a sample of pedigreed baboons, I quantify craniofacial variation to address specific questions regarding the distribution and magnitude of phenotypic, genetic, and environmental variation patterns. In addition, I identify regions of the genome containing genetic …

Genetic Imputation: Accuracy To Application, Shelina Raynell Ramnarine

Arts & Sciences Graduate Student Theses and Dissertations

Genotype imputation, the process of inferring genotypes for untyped variants, is used to identify and refine genetic association findings. This body of work focuses on assessing imputation accuracy and uses imputed data to identify genetic contributors to mentholated cigarette preference. Inaccuracies in imputed data can distort the observed association between variants and a disease. Many statistics are used to assess accuracy; some compare imputed to genotyped data and others are calculated without reference to true genotypes. Prior work has shown that the Imputation Quality Score (IQS), which is based on Cohens kappa statistic and compares imputed genotype probabilities to true …

Metal Selectivity And Acquisition By The Yersiniabactin Metallophore System In Escherichia Coli, Eun-Ik Koh

Arts & Sciences Graduate Student Theses and Dissertations

Urinary tract infections (UTI) are one of the most common bacterial infections, of which, the majority are caused by uropathogenic Escherichia coli (UPEC). Many bacterial pathogens including UPEC synthesize and secrete chemically diverse metabolites called siderophores, which are classically defined by their ability of bind and deliver iron(III), an essential nutrient, to pathogens during infections. UPEC isolates can express multiple siderophore systems, of which, the virulence-associated siderophore yersiniabactin (Ybt) binds to both iron(III) and copper(II) during urinary tract infections. In this thesis we show that Ybt interacts with multiple physiologic transition metals and acts as a metallophore system to deliver …

The Resolution Phase Of Nk Cell Proliferation And Ifn Production Following Viral Infection Are Highly Regulated Processes., Leslie Abigail Fogel

Arts & Sciences Graduate Student Theses and Dissertations

In response to MCMV infection, NK cells undergo three distinct phases of proliferation: the non-specific phase mediated by pro-proliferative cytokines; the specific phase mediated by recognition of an MCMV-encoded protein by an NK cell activating receptor, Ly49H; and the resolution phase, whose mechanism is unknown. MCMV infection of RAG mice, which lack all adaptive immune cells, results in prolonged proliferation of NK cells despite similar viral titers compared to wildtype mice. Interestingly, there are different kinetics for Ly49H+ and Ly49H- NK cells. We have identified several additional markers that may distinguish NK cells that have been specifically activated through their …

Rostral Middle Frontal Gyrus Thickness Is Associated With Perceived Stress And Depressive Symptomatology, Lindsay J. Michalski

Arts & Sciences Graduate Student Theses and Dissertations

Elevated stress perception and depression commonly co-occur and have shared genetic and environmental influences, suggesting they may rest upon a common underlying neurobiology. The rostral middle frontal gyrus (RMFG), part of the dorsolateral prefrontal cortex, is critical for executive function, including emotion regulation and working memory. Variability in RMFG cortical thickness has been associated with both depression and stress-related phenotypes, although the directionality of these associations has been inconsistent thus far. The current study examined healthy participants (n=879) who completed the ongoing family-based Human Connectome Project were included in analyses. RMFG cortical thickness was computed from structural magnetic resonance imaging …

Suicide Attempts From Adolescence Into Young Adulthood: A System Dynamics Perspective For Intervention And Prevention, Saras Yu Chung

Arts & Sciences Graduate Student Theses and Dissertations

Though the reduction of suicide-related deaths has been a national priority for over a decade (U.S. Department of Health and Human Services, 2001) and over $22 million per year (National Institutes of Health, 2015) have been invested to prevent suicide, rates of suicide have not declined (CDC, 2012). In fact, for some groups of adolescents, these rates seem to be on the ride (Wasserman, Cheng, & Jiang, 2005). The ineffectiveness in reducing deaths by suicide despite increased funding and coordinated efforts suggests the need for a new perspective on examining why and how adolescents begin to desire and attempt suicide …

Anxious Anticipation Of A Social Threat And Its Effect On Time Estimation, Natasha April Tonge

Arts & Sciences Graduate Student Theses and Dissertations

Time perception is a well-studied phenomenon; however, subjective experience of time and its relationship to affective states has received comparatively less attention in the literature (Grondin, 2010). Recently, it was suggested that anticipatory anxiety may also lead to an overestimation effect regarding angry faces in socially anxious individuals (Jusyte et al., 2014). In the present study, participants completed two temporal bisection tasks (TBT) in which they were asked to categorize a stimulus as being of short or long duration. Between the tasks, participants were to present a speech that served to provoke anxiety. In the present study, I aimed to …

A Social Network Analysis Of Active Transportation Policy Networks, Marissa Zwald

Arts & Sciences Graduate Student Theses and Dissertations

Background: In an effort to increase physical activity, communities are recognizing the importance of policy and environmental changes to facilitate active transportation. However, evidence on the policy partnerships and processes to achieve such policy and environmental changes, particularly in non-health sectors, is lacking. Methods: An online social network survey was administered in Fall 2015 to organizations engaged in active transportation policies in six cities across the United States. In addition to individual and organizational characteristics, relationships between organizations were assessed, including: level of collaboration around active transportation policies, frequency of contact, resource sharing to support active transportation, and perceived decisional …

Can We Walk? Environmental Supports For Active Travel In India, Deepti Adlakha

Arts & Sciences Graduate Student Theses and Dissertations

Background: Rapid rates of increase of obesity, diabetes, and associated chronic and co-morbid non-communicable diseases (e.g., cardiovascular diseases and some cancers) are being documented in India, yet in-country evidence-based research of associated risk factors is lacking. Physical activity has been identified as a preventative factor to counter the risk from obesity-related non-communicable diseases. Built environment supports for physical activity represent promising strategies to curb the rise in non-communicable diseases. Mounting research evidence suggests that the built environment can facilitate or constrain physical activity. However, a majority of this research has been conducted in developed nations. Built environment correlates of physical …

Structure And Dynamics Of A Small Multidrug Resistance Transporter, Emre, Chao Wu

Arts & Sciences Graduate Student Theses and Dissertations

EmrE is a small multidrug resistance transporter in E. coli. It effluxes a wide range of antibiotics, thus contributing to the evolving epidemic of drug resistance. Despite its small size, EmrE is a fully functional transporter making it an ideal model system for a comprehensive study of the multidrug transport mechanism. In the transport cycle, EmrE must alternate between outward- and inward-facing conformations upon substrate binding to translocate substrates across the membrane. High-resolution structures of EmrE in complex with substrates facing different sides of the membrane will shed light on the coupling mechanism between substrate binding and transport. However, the …

Role Of Atg16l1 In Uropathogenic E. Coli Pathogenesis, Jane Symington

Arts & Sciences Graduate Student Theses and Dissertations

Urinary tract infections (UTIs) are among the most common infectious diseases and are primarily caused by uropathogenic E. coli (UPEC). Given the greater incidence of antibiotic resistance among UPEC isolates, it is vital to determine factors and pathways important for an effective host response to UPEC in order to improve therapeutic options for combating UTIs. Autophagy is a cellular degradation pathway that plays important roles in pathogen control and modulation of innate immunity. One essential autophagy protein, ATG16L1, has been further implicated in controlling inflammation due to a common variant of ATG16L1 being associated with increased risk of Crohns disease, …