Digital Commons Network^™

Ectodomain Architecture Affects Sequence And Functional Evolution Of Vertebrate Toll-Like Receptors, Jinlan Wang, Zheng Zhang, Jing Liu, Jing Zhao, Deling Yin

ETSU Faculty Works

Toll-like receptors (TLRs) are crucial components of innate immunity that specifically recognize diverse pathogen-associated molecular patterns from pathogens. The continuous hydrogen-bond network (asparagine ladder) formed among the asparagine residues on the concave surfaces of neighboring leucine-rich repeat modules assists in stabilizing the overall shape of TLR ectodomains responsible for ligand recognition. Analysis of 28 types of vertebrate TLRs showed that their ectodomains possessed three types of architectures: a single-domain architecture with an intact asparagine ladder, a three-domain architecture with the ladder interrupted in the middle, and a trans-three-domain architecture with the ladder broken in both termini. Based on a phylogenetic …

Comparative Genomics Explains The Evolutionary Success Of Reef-Forming Corals, Debashish Bhattacharya, Shobhit Agrawal, Manuel Aranda, Sebastian Baumgarten, Mahdi Belcaid, Jeana L. Drake, Douglas Erwin, Sylvian Foret, Ruth D. Gates, David F. Gruber, Bishoy Kamel, Michael P. Lesser, Oren Levy, Yi Jin Liew, Matthew Macmanes, Tali Mass, Monica Medina, Shaadi Mehr, Eli Meyer, Dana C. Price, Hollie M. Putnam, Huan Qiu, Chuya Shinzato, Eiichi Shoguchi, Alexander J. Stokes, Sylvie Tambutté, Dan Tchernov, Christian R. Voolstra, Nicole Wagner, Charles W. Walker, Andreas Pm Weber, Virginia Weis, Ehud Zelzion, Didier Zoccola, Paul G. Falkowski

Publications and Research

Transcriptome and genome data from twenty stony coral species and a selection of reference bilaterians were studied to elucidate coral evolutionary history. We identified genes that encode the proteins responsible for the precipitation and aggregation of the aragonite skeleton on which the organisms live, and revealed a network of environmental sensors that coordinate responses of the host animals to temperature, light, and pH. Furthermore, we describe a variety of stress-related pathways, including apoptotic pathways that allow the host animals to detoxify reactive oxygen and nitrogen species that are generated by their intracellular photosynthetic symbionts, and determine the fate of corals …

Using Soundscape Ecology In Design And Function, Wieteke Holthuijzen

Contemporary Aesthetics (Journal Archive)

No abstract provided.

Stress-Induced Executive Dysfunction In Gdnf-Deficient Mice, A Mouse Model Of Parkinsonism, Mona Buhusi, Kaitlin Olsen, Benjamin Z. Young, Catalin V. Buhusi

Psychology Faculty Publications

Maladaptive reactivity to stress is linked to improper decision making, impulsivity, and discounting of delayed rewards. Chronic unpredictable stress alters dopaminergic function and re-shapes dopaminergic circuits in key areas involved in decision making, and impairs prefrontal-cortex dependent response inhibition and working memory. Glial-derived neurotrophic factor (GDNF) is essential for regulating dopamine release in the basal ganglia and the survival of dopaminergic neurons, and GDNF-deficient mice are considered an animal model for aging-related Parkinsonism. Recently, GDNF expression in the striatum has been linked to resilience to stress. Here we investigated the effects of chronic unpredictable stress on decision making in GDNF-heterozygous …

A Surprisingly Poor Correlation Between In Vitro And In Vivo Testing Of Biomaterials For Bone Regeneration: Results Of A Multicentre Analysis, G. Hulsart-Billström, J. I. Dawson, S. Hofmann, R. Müller, M. J. Stoddart, M. Alini, H. Redl, Haj A El, R. Brown, V. Salih, J. Hilborn, S. Larsson, Richard O.C. Oreffo

Peninsula Dental School

No abstract provided.

Thingbook: The Society Of All Things (Humans, Animals, Things And Data), G Corino, M Phillips

School of Art, Design and Architecture

In considering the origin of Things, it is quite conceivable that a designer, reflecting on the mutual affinities of organic and inorganic beings, on their morphological relations, their geographical distribution, cultural succession, social function and other such facts, might come to the conclusion that things had not been independently created, but had descended, like varieties, from other Things. Nevertheless, such a conclusion, even if well founded, would be unsatisfactory, until it could be shown how the innumerable Things, inhabiting this world have been modified, so as to acquire that perfection of structure and coadaptation which justly excites our imagination. In …

Measurement Of The Spatial Distribution Of Mechanical Strain In Alveolar Epithelial Cell Monolayers, Joshua Herwig

Electronic Theses and Dissertations Archive

Measuring the inter- and intra- cellular mechanical response of single cell-thick monolayers to mechanical and biochemical stimulation is a complex and challenging problem. As a result, very few investigative methods exist. In this work, a robust, and proven method for determining mechanical strains is employed to address this fundamental unmet need. This work is motivated by the study of the lung alveolar epithelium, a vital and dynamic cell monolayer, which, in the diseased lung, is challenged by both potentially injurious levels of distention and also harmful pro-inflammatory immune-modulating chemicals. Previous work has demonstrated that harmful mediators (hyperoxia and TNF-α) tend …

A Tale Of Two Mommies: (Re)Storying Family Of Origin Narratives, Elizabeth A. Suter, Jody Koenig Kellas, Stephanie K. Webb, Jordan A. Allen

Department of Communication Studies: Faculty Publications

This study examined co-mother family of origin stories. Origin stories, representing the formation of a family, are culturally understood within a master narrative of heterosexual love and biological childbearing. Beginnings of co-mother families rupture this dominant, gendered, boy-meets-girl script. Investigating whether or not co-mother stories reify the normative master narrative or if instead their narrations resist and/or possibly transform conventional understandings, analysis identified three co-mother origin story themes: Becoming a Family (1) as Normal, (2) as Negotiation, and (3) as Normalization. Themes differ in terms of depiction of co-mother family formation as congruent with current norms, as something that needs …

Collybolide Is A Novel Biased Agonist Of Κ-Opioid Receptors With Potent Antipruritic Activity, Achla Gupta, Ivone Gomes, Erin N. Bobeck, Amanda K. Fakira, Nicholas P. Massaro, Indrajeet Sharma, Adrien Cavé, Heidi E. Hamm, Joseph Parello, Lakshmi A. Devi

Biology Faculty Publications

Among the opioid receptors, the κ-opioid receptor (κOR) has been gaining considerable attention as a potential therapeutic target for the treatment of complex CNS disorders including depression, visceral pain, and cocaine addiction. With an interest in discovering novel ligands targeting κOR, we searched natural products for unusual scaffolds and identified collybolide (Colly), a nonnitrogenous sesquiterpene from the mushroom Collybia maculata. This compound has a furyl-δ-lactone core similar to that of Salvinorin A (Sal A), another natural product from the plant Salvia divinorum. Characterization of the molecular pharmacological properties reveals that Colly, like Sal A, is a highly potent and selective …

Split Wounds: Diverging Formations Of Trauma In The Diagnostic And Statistical Manual Of Mental Disorders V, Girl With The Dragon Tattoo, And The Rat Laughed, And Once Were Warriors, Emily R. Johnston

Theses and Dissertations

Split Wounds interrogates naturalized, normalized trauma wisdom—particularly the individualization and pathologization of sexualized trauma. Drawing on Foucault’s concept of discursive formation, explicated in The Archaeology of Knowledge as a set of conditions that enables history, this dissertation elucidates differing discursive formations of trauma in contemporary medical documents, literary texts, and films. The introductory chapter explicates how founding texts in the field of trauma theory construct trauma as a preverbal, psychological experience that can only be represented through fragmented, non-linear, anti-narrative textual strategies. Chapter two exposes such Euro-American modernist ideology in the American Psychiatric Association’s clinical definition of posttraumatic stress disorder …

Viability Of Rickettsia Prowazekii After Rickettsia-Mediated Killing Of Gamma Interferon-Pretreated, Macrophage-Like Raw264.7 Cells, Kathryn H. Vernon, Jenifer Turco

Georgia Journal of Science

Rickettsia prowazekii, the bacterium that causes epidemic typhus, mainly grows within endothelial cells and can also infect macrophages. Previous studies showed that pretreatment of cultured, murine macrophage-like RAW264.7 cells with gamma interferon, followed by R. prowazekii infection, leads to the death of many of the macrophages within several hours after infection. The present study examined the fate of the rickettsiae after macrophage death. Rickettsiae released from gamma interferon-pretreated, infected macrophage cultures, in which 83 ± 4% (mean ± standard deviation) of the macrophages were trypan blue-positive (dead), remained viable, as judged by their ability to infect and grow in …

The Relationship Between Fluidized Bed Electrostatics And Entrainment, John R. Grace, Turki Alsmari, Xiaotao Bi, Farzam Fotovat

Fluidization XV

Existing methods of predicting entrainment from gas-fluidized beds, based exclusively on hydrodynamic variables and mechanisms, show extremely wide discrepancies in their predictions and often deviate by many orders of magnitude from experimental values. Based on simultaneous measurement of electrostatic charges in the freeboard region and entrainment, this paper will show that inter-particle electrostatic forces on relatively fine particles can be at least of the same order of magnitude as the gravity forces that must be overcome for entrainment to take place. Moreover, incorporation of a term for inter-particle electrostatic force leads to substantial improvement in the ability to correlate our …

Solid Flux In Travelling Fluidized Bed Operating In Square-Nosed Slugging Regime, Sina Tebianian, Kristian Dubrawski, Naoko Ellisa, John R. Grace, Thomas W. Leadbeater, David J. Parker, Rouzbeh Jafari, Jamal Chaouki, Pablo Garcia-Trinanes, Jonathan P. K. Seville

Fluidization XV

The performance of gas-fluidized bed reactors depends significantly on their hydrodynamics. Among the important properties that dictate the characteristics of a gas-fluidized bed, local solid flux plays a significant role, influencing vital parameters such as bed-to-surface heat exchange and solid circulation rate. Developing techniques that can provide accurate measurements of solid flux is extremely important for: 1) assessing the accuracy of other measurement techniques applicable to industrial units, and 2) validation of CFD models.

Comparison of different measurement techniques that provide similar hydrodynamic information is helpful in assessing the errors associated with each methodology. Most measurement techniques for obtaining solid …

Nutrient Recycling By Eared Grebes In The Great Salt Lake, Anthony J. Roberts, Michael R. Conover

Wildland Resources Faculty Publications

Nitrogen (N) and phosphorus (P) cycling in lakes can be impacted by numerous abiotic and biotic factors. Large animals, such as birds and mammals, may recycle nutrients within a system or bring them in from surrounding habitats or different landscapes. Eared Grebes (Podiceps nigricollis) congregate in large numbers in the fall on the Great Salt Lake (GSL), Utah, and may have an important role in recycling nutrients from macroinvertebrates back into the water column. We evaluated the role of Eared Grebes in N and P cycling within the GSL by estimating the magnitude of their nutrient recycling capacity. We estimated …

Full Issue - Alpenglow 2016, Undergraduate Center

Binghamton University Undergraduate Journal

Full issue of Alpenglow 2016

Acknowledgments, Editorial Board, Review Team, And About Cover Art

Binghamton University Undergraduate Journal

Acknowledgment of persons who have been instrumental to the publication of the journal, list of editorial board and review team, and a brief discussion about the cover art.

Stem School Discourse Patterns, Colby Tofel-Grehl, Carolyn M. Callahan

Teacher Education and Leadership Faculty Publications

In a cross case analysis of six specialized STEM schools across the United States selected on the basis of varied geographic location and school model type, the authors explore the ways in which classroom discursvive strutures established by teachers impact students' perceptions of their own agency and authority as relates to their science learning within specialized STEM schools. As national interest in specialized STEM schools grows, a deep need to understand the instructional practices that impact students motivation and interest has arisen. By better understanding how teachers drive classroom discourse through questioning techniques, student responses and reflections can be understood …

Investigating The Effects Of 17Α-Ethynylestradiol On Mitochondrial Genome Stability, Alicia M. Chivers

Dissertations and Theses

Environmental toxicants are ubiquitous throughout the environment as a result of human activity. Among these toxicants, environmental estrogens are a category of particular concern due to their environmental prevalence and potency in altering reproductive traits. While many studies have addressed the detrimental effects of environmental estrogens on both aquatic and terrestrial organisms, few have analyzed the potential for these compounds to alter mitochondrial function. Mitochondria are the primary energy-generating system for all eukaryotic life, supporting all aspects of development, metabolism, and growth. Each cell within the body contains many mitochondria which in turn contain multiple copies of their own DNA …

Reengineering Of Mesh Thesauri For Term Selection To Optimize Literature Retrieval And Knowledge Reconstruction In Support Of Stem Cell Research, Yan Su, James E. Andrews, Hong Huang, Yue Wang, Liangliang Kong, Peter Cannon, Ping Xu

School of Information Faculty Publications

BACKGROUND: PubMed is a widely used database for scientists to find biomedical-related literature. Due to the complexity of the selected research subject and its interdisciplinary nature, as well as the exponential growth in the number of disparate pieces of biomedical literature, it is an overwhelming challenge for scientists to define the right search strategies and quickly locate all related information. Specialized subsets and groupings of controlled vocabularies, such as Medical Subject Headings (MeSH), can enhance information retrieval in specialized domains, such as stem cell research. There is a need to develop effective search strategies and convenient solutions for knowledge organization …

Unh Welcomes 800+ Fourth Graders To Fairchild Dairy June 7-9, Lori Wright

Media Relations

No abstract provided.

Food Studies Celebrates Graduates Awaits Approval For New Certificate, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Multiple Sequence Alignment With Pro Le Hidden Markov Models, Shubhangi Rakhonde

Master's Projects

The human genome consists of various patterns and sequences that are of biolog- ical signi cance. Capturing these patterns can help us in resolving various mysteries related to the genome, like how genomes evolve, how diseases occur due to genetic mutation, how viruses mutate to cause new disease and what is the cure for these diseases. All these applications are covered in the study of bioinformatics.

One of the very common tasks in bioinformatics involves simultaneous alignment of a number of biological sequences. In bioinformatics, this is widely known as Mul- tiple Sequence Alignment. Multiple sequence alignments help in grouping …

James Robert Rawcliffe, Plaintiff And Appellant, V, Robert Anciaux, Et Al., Defendants And Appellees., Utah Court Of Appeals

Utah Court of Appeals Briefs (2007– 2019)

Appeal from the Third District Court, Salt Lake County, State of Utah The Honorable Heather Brereton, District Court No. 140905252

Symbiosis Now, Priscilla Fusco

Theses and Dissertations

As we narrow the world’s parameters with our activities, we crowd other organisms that support us. Scientists and philosophers including Charles Sanders Peirce, Lynn Margulis, Thomas Khun and Dave Goulson articulate the role of the biomass, an intelligence that offers fertile dialogue for sculptors.

Navel Gazing, Andrew J. Macasil

Theses and Dissertations

The purpose of this project is to present work that disrupts the heteronormative binary that has dominated the representation of the human form in painting. The navel is employed as a bodily metaphor to address the universality of unique bodily experiences, which are tied to one’s identity.

Statement, Elektra Kuznetsova Blanco

Theses and Dissertations

Elektra KB's statement of art practice. Her work uses a utopic-dystopic narrative construct between documentary and fantasy as a platform to investigate and reflect upon power struggles, sense of place, migration, gender, oppression, violence, and the spectaclist technological society. It draws a tension between different ‘realities’ aiming to deconstruct the language of neo-liberalism. The theocratic Republic of Gaia, an alternative world created as a safe space of resistance, and inhabited by its rebel autonomous counterparts The Catharas, is experiencing great social, geological and political upheaval.

Quantitative Evidence For The Effects Of Multiple Drivers On Continental-Scale Amphibian Declines, Evan H. Campbell Grant, David A. W. Miller, Benedikt R. Schmidt, Michael J. Adams, Staci M. Amburgey, Thierry Chambert, Sam S. Cruickshank, Robert N. Fisher, David M. Green, Blake R. Hossack, Pieter T. J. Johnson, Maxwell B. Joseph, Tracy A. G. Rittenhouse, Maureen E. Ryan, J. Hardin Waddle, Susan C. Walls, Larissa L. Bailey, Gary M. Fellers, Thomas A. Gorman, Andrew M. Ray, David S. Pilliod, Steven J. Price, Daniel Saenz, Walt Sadinski, Erin Muths

Forestry and Natural Resources Faculty Publications

Since amphibian declines were first proposed as a global phenomenon over a quarter century ago, the conservation community has made little progress in halting or reversing these trends. The early search for a “smoking gun” was replaced with the expectation that declines are caused by multiple drivers. While field observations and experiments have identified factors leading to increased local extinction risk, evidence for effects of these drivers is lacking at large spatial scales. Here, we use observations of 389 time-series of 83 species and complexes from 61 study areas across North America to test the effects of 4 of the …

Multi-Regression Prediction Of Metal Partition Coefficients Under Various Physical/Chemical Conditions Design Of Experiments As, Cr, Cu, Ni And Zn, Eid A. Alkhatib, Danielle Grunzke, Trey Chabot

Chemistry & Physics Faculty Publications

The behavior of metals in surface water is complex and their partition coefficients can be impacted by many factors. Organic matter (OM) content in sediments, pH and salinity, are factors that may influence speciation and partitioning of metals. The difficulty in describing the impacts and relationships are that these processes are interconnected with no dominant associations among all. In this study, the partitioning of five metals (As, Cr, Cu, Ni and Zn) under different levels of salinity, pH, and OM content were investigated. A series of factorial design experiments are evaluated in which three levels of OM are tested each …

Genetic Manipulation Of Iron Biomineralization Enhances Mr Relaxivity In A Ferritin-M6a Chimeric Complex, Marina Radoul, Limor Lewin, Batya Cohen, Roni Oren, Stanislav Popov, Geula Davidov, Moriel H. Vandsburger, Alon Harmelin, Ronit Bitton, Jean-Marc Greneche, Michal Neeman, Raz Zarivach

Physiology Faculty Publications

Ferritin has gained significant attention as a potential reporter gene for in vivo imaging by magnetic resonance imaging (MRI). However, due to the ferritin ferrihydrite core, the relaxivity and sensitivity for detection of native ferritin is relatively low. We report here on a novel chimeric magneto-ferritin reporter gene – ferritin-M6A – in which the magnetite binding peptide from the magnetotactic bacteria magnetosome-associated Mms6 protein was fused to the C-terminal of murine h-ferritin. Biophysical experiments showed that purified ferritin-M6A assembled into a stable protein cage with the M6A protruding into the cage core, enabling magnetite biomineralisation. Ferritin-M6A-expressing C6-glioma cells showed enhanced …

Exploring Alternate Route Science Teacher Development Of Pedagogical Content Knowledge, Kim Feltre

Theses and Dissertations

The purpose of this qualitative case study was to understand how alternate route first year science teachers’ learning experiences gained from their alternate route courses translated into their classroom instruction and facilitated the development of their pedagogical content knowledge. Participants included three first year high school teachers from two alternate route program institutions. Data collection and analyses focused on semi-structured interviews, classroom observations, and teacher-generated artifacts. Findings reveal patterns among participant responses that emphasized the limited translation of learned pedagogical experiences from their alternate route programs into classroom instruction. In addition, findings show that participant alternate route program learned pedagogical …